Application of GGTI298 in prevention and control of rice blast

Abstract

The invention discloses application of GGTI298 in prevention and control of rice blast, and belongs to the technical field of rice blast prevention and control. According to the technical scheme, the novel medical application of the GGTI298 is disclosed, and the GGTI298 has good antibacterial activity on magnaporthe oryzae and can inhibit conidia germination and appressorium formation of the magnaporthe oryzae so as to influence pathogenicity. The GGTI298 has a prospect of preventing and treating magnaporthe oryzae infection, and a reference basis is provided for development of antibacterial drugs based on a GGTI298 pilot structure.

Description

Technical Field

[0001] The invention belongs to the technical field of rice blast prevention and control, and particularly relates to the application of GGTI298 in preventing and controlling rice blast. Background Art

[0002] Rice blast is a disease caused by Magnaporthe oryzae that seriously threatens rice production. It can cause a large reduction in crop yields and affect global food security. By screening effective control compounds, we can not only find new ways to control rice blast and reduce dependence on traditional chemical pesticides, but also promote the development of environmentally friendly agriculture. The application of efficient and low-toxic control compounds can significantly reduce crop losses, increase yields, and reduce the impact on the ecosystem. This not only helps to ensure food supply, but also helps to increase farmers' income and promote sustainable agricultural practices. Therefore, continuous research and screening of new control compounds is an important part of ensuring the healthy development of agriculture.

[0003] GGTI298 Trifluoroacetate (hereinafter referred to as GGTI298) is an effective inhibitor of geranyl pyrophosphate transferase I (GGTase I), with CAS number: 1217457-86-7, and its chemical structure is:

[0004]

[0005] GGTI298 is used in cell biology research to investigate the process of protein prenylation, a post-translational modification of proteins that is essential for the function of certain G proteins. By inhibiting GGTase I, GGTI298 is able to prevent the prenylation of proteins such as Rap1A. This inhibitor is currently a research compound and is commonly used in laboratory settings to study signaling pathways that rely on prenylation and to explore potential therapeutic strategies for diseases that involve abnormal signaling, such as cancer. There are currently no reports of its use in controlling plant diseases. Summary of the invention

[0006] In view of the problems existing in the prior art, the purpose of the present invention is to design and provide a technical solution for the application of GGTI298 in preventing and controlling rice blast.

[0007] The present invention is specifically implemented by the following technical solutions:

[0008] The first aspect of the present invention provides use of GGTI298 in the preparation of antifungal drugs, wherein the CAS number of GGTI298 is: 1217457-86-7.

[0009] Furthermore, the fungus is rice blast fungus.

[0010] The second aspect of the present invention provides the use of GGTI298 in the preparation of drugs for preventing and controlling rice blast, and the CAS number of GGTI298 is: 1217457-86-7.

[0011] A third aspect of the present invention provides a drug for preventing and controlling rice blast, wherein the active ingredient of the drug contains GGTI298.

[0012] Furthermore, the rice blast disease is caused by Magnaporthe oryzae.

[0013] Furthermore, the medicine is an injection, tablet, pill, capsule, suspension, granule, spray or emulsion.

[0014] The fourth aspect of the present invention provides the use of the above-mentioned drug in preventing and controlling rice blast.

[0015] The technical solution of the present invention discloses a new medical use of GGTI298. GGTI298 has good antibacterial activity against rice blast fungus, can inhibit the germination of conidia and the formation of appressorium of rice blast fungus, and thus affect the pathogenicity. GGTI298 has the prospect of being used to prevent and treat rice blast fungus infection, and provides a reference for the development of antibacterial drugs based on the lead structure of GGTI298. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a statistical result diagram of the effect of GGTI298 on the growth and spore production of rice blast fungus;

[0017] Figure 2 This is a statistical result diagram of GGTI298 inhibiting the pathogenicity of rice blast fungus;

[0018] Figure 3 This is the statistical result of GGTI298 inhibiting conidia germination and appressorium formation of rice blast fungus. DETAILED DESCRIPTION

[0019] The present invention is further described below with reference to the examples.

[0020] Example 1: GGTI298 does not affect the growth and spore production of rice blast fungus

[0021] The rice blast fungus B157 cake with a diameter of 1 mm was inoculated onto complete medium plates containing 1, 5, 10, 20, and 50 μM GGT1298. The plates without the addition of the agent were used as controls. The plates were placed in an incubator at 25°C. After 6 days of culture under alternating light and dark conditions, the colony diameter and the number of conidia were counted. Each treatment was repeated three times. The diameter measurement results and the plate conidia statistics showed that ( Figure 1As shown), the addition of GGTI298 to the complete culture did not affect the growth of the rice blast fungus. In addition, statistics on the spore production on the plates showed that there was no significant difference in the spore production of the rice blast fungus on the plates containing different concentrations of the agent.

[0022] Example 2: GGTI298 inhibits the pathogenicity of rice blast fungus

[0023] The rice blast fungus strain B157 was cultured in a 25°C incubator under alternating dark / light conditions for 6 days. The colonies were washed with sterile water, and the conidia on the plate were collected. The spore solution concentration was adjusted to 1×10 6 After adding 10μM GGTI298 to the conidia of rice blast fungus, the pathogenicity of 20 microliters of conidia to barley leaves was detected by in vitro inoculation. After inoculation, the conidia were first treated at 22℃ in the dark for 2 days, and then cultured at 25℃ in alternating light / dark conditions for 5 days. The results showed that the area of ​​lesions after adding 10μM GGTI298 was significantly smaller than that of the control group, indicating that the pathogenicity of rice blast fungus was significantly reduced (such as Figure 2 Then we inoculated rice seedlings of rice blast susceptible variety CO39 four weeks old by live spraying, and used DMSO-added rice blast fungus spore solution as control. After inoculation, we first treated them in dark conditions at 22℃ for 2 days, and then cultured them in alternating light / dark conditions at 25℃ for 5 days. After 7 days, we took pictures and counted the leaf spot area using Photoshop software. The results showed that (as shown in Figure 2 As shown in the figure, yellow and brown lesions appeared on the leaves of the control group, but the lesion area of ​​the experimental group after adding 10 μM GGTI298 was significantly smaller. These results show that GGTI298 has a significant inhibitory effect on the pathogenicity of rice blast fungus.

[0024] Example 3: GGTI298 inhibits conidia germination and appressorium formation of Magnaporthe oryzae

[0025] Appressorium is a specific structure of rice infected by rice blast fungus. Its formation rate is closely related to the pathogenicity of rice blast fungus. Therefore, we observed the effect of GGTI298 on the formation of appressorium of rice blast fungus by microscopy. Conidia of rice blast fungus were collected from the plates where rice blast fungus was cultured for 7 days. The concentration of spore solution was measured by hemocytometer and the concentration of spore solution was adjusted to 1×10 5 10 μM GGTI298 was added to the conidia of rice blast fungus, and the same volume of DMSO was added as a control. The conidia were dropped on the surface of a hydrophobic glass slide and incubated in a dark and moisturizing environment at 25°C. After 4 hours, the germination of conidia was counted under a microscope, and the formation rate of conidial appressorium was counted after 24 hours. The results show (eg Figure 3After 4 hours of incubation, the conidia germination rate of the 10 μM GGTI298-treated conidia was 80.3% ± 5%, slightly lower than the control group 98.6 ± 2%, and after 24 hours, the appressorium formation rate of the 10 μM GGTI298-treated conidia was 0%, significantly lower than the control group 96.5% ± 3%. These results indicate that 10 μM GGTI298 specifically inhibits the formation of appressorium and thus inhibits the pathogenicity of the rice blast fungus.

Claims

1. Application of GGTI298 in the preparation of antifungal drugs, wherein the CAS number of GGTI298 is: 1217457-86-7.

2. The use according to claim 1, characterized in that The fungus is rice blast fungus.

3. Application of GGTI298 in the preparation of drugs for preventing and controlling rice blast, wherein the CAS number of GGTI298 is: 1217457-86-7.

4. A drug for preventing and controlling rice blast, characterized in that: The active ingredient of this medicine contains GGTI298.

5. A drug for preventing and controlling rice blast as claimed in claim 4, characterized in that: The rice blast disease is caused by Magnaporthe oryzae.

6. A drug for preventing and controlling rice blast as claimed in claim 4, characterized in that: The medicine is in the form of injection, tablet, pill, capsule, suspension, granule, spray or emulsion.

7. Use of the drug according to claim 4, 5 or 6 in preventing and controlling rice blast.

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