Black propolis tea extract composition with nourishing and anti-aging effects and application thereof

Abstract

The invention relates to the field of cosmetics, in particular to a black propolis tea extract composition with nourishing and anti-aging effects and application of the black propolis tea extract composition. The black propolis tea extract composition is prepared from the following components in parts by weight: 0.001 to 0.2 part of EGCG (epigallocatechin gallate), 1 to 5 parts of royal jelly extract and 0.5 to 5 parts of black propolis extract. After the EGCG, the royal jelly extract and the black propolis extract are compounded, a synergistic interaction effect is achieved, the maximum skin care effect can be achieved, and the skin care composition has the nourishing and anti-aging effects when being applied to cosmetics.

Description

Technical Field

[0001] The invention relates to the field of cosmetics, and in particular to a black propolis tea extract composition with nourishing and anti-aging effects and application thereof. Background Art

[0002] Black propolis is a unique propolis extracted from European wasps. It has high nutritional and medicinal value and is an excellent health product. Black propolis generally contains flavonoids, which can have an antioxidant effect, remove excess free radicals in the body, and accelerate metabolism.

[0003] Royal jelly is a bee product that is secreted by 5-15 day old worker bees. It is composed of water, protein, sugar, lipid, acid, trace elements and other natural components. It is a raw material with strong biological activity and has a wide range of application prospects in food, medical materials, health products and cosmetics. Royal jelly has antioxidant properties and can inhibit the production of ROS and the expression of NADPH oxidase 4.

[0004] EGCG (epigallocatechin gallate) is derived from tea extract and is a natural polyphenol compound. In cosmetics, EGCG exhibits excellent antioxidant properties, which can effectively resist the damage of free radicals to the skin and slow down the aging process of the skin. At the same time, it also has anti-inflammatory and antibacterial properties, which helps maintain the health of the skin and improve the condition and radiance of the skin.

[0005] The application of the above components in cosmetics is mostly limited and their skin care value has not been brought into play. Summary of the invention

[0006] The purpose of the present invention is to overcome the shortcomings of the prior art and provide a black propolis tea extract composition with nourishing and anti-aging effects.

[0007] To achieve the above object, the technical solution adopted by the present invention is:

[0008] In a first aspect, the present invention provides a black propolis tea extract composition, comprising the following components in parts by weight: 0.001-0.2 parts of EGCG, 1-5 parts of royal jelly extract, and 0.5-5 parts of black propolis extract.

[0009] EGCG, royal jelly extract, and black propolis extract all have antioxidant properties, but are more limited in skin care applications. Black propolis and royal jelly are relatively precious because of their difficulty in extraction, and are relatively blank in cosmetic applications, and their skin care value has not yet been brought into play. Although EGCG has a good skin care effect, it is relatively limited in application concentration due to its poor stability, and its maximum skin care effect cannot be brought into play. After the three components of EGCG, royal jelly extract, and black propolis extract are compounded within a certain range, the present invention has a synergistic effect, can bring out the maximum skin care effect, and is applied in cosmetics with strong nourishment and strong anti-aging efficacy, bringing new possibilities for the application of cosmetics.

[0010] Preferably, the black propolis tea extract composition comprises the following components in parts by weight: 0.05-0.2 parts of EGCG, 1-5 parts of royal jelly extract, and 1-5 parts of black propolis extract.

[0011] More preferably, the black propolis tea extract composition comprises the following components in parts by weight: 0.2 parts of EGCG, 5 parts of royal jelly extract, and 5 parts of black propolis extract.

[0012] In a second aspect, the present invention provides the use of the above-mentioned black propolis tea extract composition in cosmetics.

[0013] In a third aspect, the present invention provides a cosmetic comprising the above-mentioned black propolis tea extract composition.

[0014] Preferably, the black propolis tea extract composition is added in an amount of 1.501%-10.2% in cosmetics.

[0015] More preferably, the black propolis tea extract composition is added in an amount of 10.2% in cosmetics.

[0016] Preferably, the dosage form of the cosmetic includes water, lotion, and cream.

[0017] Preferably, the cosmetics also include auxiliary materials acceptable in daily chemical products.

[0018] Preferably, the auxiliary material includes at least one of an emollient, a dispersant, a humectant, a preservative, an emulsion stabilizer, a thickener, and a pH adjuster.

[0019] The beneficial effects of the present invention are:

[0020] EGCG, royal jelly extract, and black propolis extract all have antioxidant properties, but are more limited in skin care applications. Black propolis and royal jelly are relatively precious because of their difficulty in extraction, and are relatively blank in cosmetic applications, and their skin care value has not yet been brought into play. Although EGCG has a good skin care effect, it is relatively limited in application concentration due to its poor stability, and its maximum skin care effect cannot be brought into play. After the three components of EGCG, royal jelly extract, and black propolis extract are compounded within a certain range, the present invention has a synergistic effect, can bring out the maximum skin care effect, and is applied in cosmetics with strong nourishment and strong anti-aging efficacy, bringing new possibilities for the application of cosmetics. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is a sample solution of the black propolis tea extract composition prepared by the present invention; from left to right are samples prepared in Example 2, Example 6, and Example 10 respectively. DETAILED DESCRIPTION

[0022] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0023] The black propolis extract used in the present invention is purchased from Swiss Midi-Bier Biotechnology Company.

[0024] The royal jelly extract used in the present invention is purchased from BASF.

[0025] The EGCG used in the present invention was purchased from Xiyuan Biotechnology Co., Ltd.

[0026] The honey extract used in the present invention is purchased from Swiss Midi-Baiyer Biotechnology Company.

[0027] The cosmetics prepared by the present invention are left to stand after the preparation is completed and discharged, and semi-finished product inspection is carried out; after passing the inspection, they are filled and packaged; and finally, the finished product inspection is carried out to obtain the final product.

[0028] Embodiment 1-12:

[0029] An embodiment of the black propolis tea extract composition of the present invention; the components by weight of the black propolis tea extract composition are shown in Table 1.

[0030] Table 1 Components by weight of the black propolis tea extract composition described in each embodiment

[0031]

[0032] Comparative Examples 1-5:

[0033] Comparative example of the black propolis tea extract composition of the present invention; the components by weight of the black propolis tea extract composition are shown in Table 2.

[0034] Table 2 Components by weight of the black propolis tea extract composition of each comparative example

[0035]

[0036]

[0037] Among them, the only difference between Comparative Examples 1-3 and Example 10 is that: Comparative Example 1 does not add EGCG; Comparative Example 2 does not add royal jelly extract; Comparative Example 3 does not add black propolis extract; and the missing components are replaced by equal weight portions of water.

[0038] The difference between Comparative Example 5 and Example 10 is that the royal jelly extract in Comparative Example 5 is replaced by an equal weight portion of honey extract purchased from MidiBier Biotechnologies of Switzerland.

[0039] Application Examples 1-12:

[0040] Application examples of the cosmetics of the present invention; the cosmetics are specifically an emulsion; the cosmetics include a black propolis tea extract composition. In each application example, the mass percentage of the black propolis tea extract composition in the cosmetics is shown in Table 3.

[0041] Table 3 Content of black propolis tea extract composition added in each application example

[0042]

[0043] The formula of the cosmetic is shown in Table 4.

[0044] Table 4 Cosmetic formula

[0045]

[0046] The preparation method of the cosmetic comprises the following steps:

[0047] (1) Add acrylic acid (esters) / vinyl isodecanoate cross-linked polymer, disodium EDTA, allantoin, glycerin, sodium hyaluronate, xanthan gum and appropriate amount of water to an emulsifier in sequence, homogenize at 2000-2500 rpm for 2-3 min, stir at 800-1200 rpm, heat to 80-85°C, homogenize at 2000-2500 rpm for 1-2 min, stir at 800-1200 rpm, keep warm and stir for 15 min, and stir until dissolved evenly.

[0048] (2) Add cyclopentasiloxane, caprylic / capric triglyceride, and C20-24 alkyl polydimethylsiloxane to the oil phase pot, stir at 500-800 rpm, and heat to 80-85° C.; pump the raw materials in the oil phase pot into the emulsifier pot, homogenize at 2200-2800 rpm for 3-5 minutes, and stir.

[0049] (3) Add pre-stirred polyacrylamide, C13-14 isoparaffin, laureth-7 and appropriate amount of water, homogenize at 2000-2500 rpm for 3-5 minutes until completely dissolved, and start cooling.

[0050] (4) Cool down to 50-55°C, add pre-dissolved (heat up to 60°C to dissolve) p-hydroxyacetophenone and butanediol, and stir at 800-1200 rpm for 3-5 minutes until the mixture is evenly dissolved.

[0051] (5) Cooling to 45° C., adding the pre-dispersed 1,2-hexanediol, black propolis tea extract composition and the remaining water in sequence, and stirring at 600-1000 rpm.

[0052] (6) cooling to 38° C., passing the central control, filtering the material (100 mesh filter bag) to obtain the cosmetics.

[0053] (7) After discharging, let the material stand and inspect the semi-finished product.

[0054] (8) After passing the inspection, filling and packaging.

[0055] (9) Inspection of finished emulsion products.

[0056] Application Comparative Example 1:

[0057] An application comparison example of the cosmetics of the present invention; the cosmetics are specifically an emulsion; the difference between this application comparison example and application example 10 is only that: the black propolis tea extract composition of example 10 contained in application example 10 is replaced with an equal amount of the black propolis tea extract composition of comparison example 1, and the remaining components are the same as application example 10.

[0058] The preparation method of the cosmetic is the same as that of the application example.

[0059] Application Comparative Example 2:

[0060] An application comparison example of the cosmetics of the present invention; the cosmetics are specifically an emulsion; the difference between this application comparison example and application example 10 is only that: the black propolis tea extract composition of example 10 contained in application example 10 is replaced with an equal amount of the black propolis tea extract composition of comparison example 2, and the remaining components are the same as application example 10.

[0061] The preparation method of the cosmetic is the same as that of the application example.

[0062] Application Comparative Example 3:

[0063] An application comparison example of the cosmetics of the present invention; the cosmetics are specifically an emulsion; the difference between this application comparison example and application example 10 is only that: the black propolis tea extract composition of example 10 contained in application example 10 is replaced with an equal amount of the black propolis tea extract composition of comparison example 3, and the remaining components are the same as application example 10.

[0064] The preparation method of the cosmetic is the same as that of the application example.

[0065] Application Comparative Example 4:

[0066] An application comparison example of the cosmetics of the present invention; the cosmetics are specifically an emulsion; the difference between this application comparison example and application example 10 is only that: 10.2% of the black propolis tea extract composition in application example 10 is replaced with 14.3% of the black propolis tea extract composition of comparison example 4, and the remaining components are the same as application example 10.

[0067] The preparation method of the cosmetic is the same as that of the application example.

[0068] Application Comparative Example 5:

[0069] An application comparison example of the cosmetics of the present invention; the cosmetics are specifically an emulsion; the difference between this application comparison example and application example 10 is that: 5% of the royal jelly extract in application example 10 is replaced by 10.2% of the honey extract in comparison example 5, and the remaining components are the same as application example 10.

[0070] The preparation method of the cosmetic is the same as that of the application example.

[0071] Application Comparative Example 6:

[0072] An application comparison example of the cosmetics described in the present invention; the cosmetics are specifically an emulsion; the difference between this application comparison example and application example 10 is only that: 10.2% of the black propolis tea extract composition in application example 10 is replaced with an equal amount of water, and the remaining components are the same as application example 10.

[0073] The preparation method of the cosmetic is the same as that of the application example.

[0074] Test Example 1: Determination of physical and chemical indicators

[0075] Test samples: 3 batches of black propolis tea extract compositions prepared in Example 10.

[0076] The conductivity color was measured by a conductivity tester; the sample was poured into a transparent sample bottle to observe the color of the composition, and then the refractive index was measured by a refractometer. The test results are shown in Table 5.

[0077] Table 5

[0078] sample Conductivity color Batch 1 466 Orange Batch 2 453 Orange Batch 3 470 Orange

[0079] The results show that the physical and chemical indicators and appearance indicators of the black propolis tea extract composition prepared by the present invention in actual production all meet the standards.

[0080] Test Example 2: Safety Patch Test

[0081] Test samples: black propolis tea extract compositions prepared in Examples 1-12 and Comparative Examples 1-5.

[0082] Subjects: 9 males and 26 females, a total of 35 subjects, aged 22 to 45 years old, with an average age of 28.1±5.6 years old, who met the voluntary inclusion criteria.

[0083] The spot test method is as follows:

[0084] Select qualified patch test equipment and use the closed patch test method to place the patch product containing the test sample (cut into the size of the patch tester, about 50mm 2 ) was placed in a patch tester, and a hypoallergenic tape was applied to the back of the subject. After 24 hours, the test sample was removed, and the skin reaction was observed at 0.5, 24, and 48 hours after removal. A negative control (blank, no treatment) was set up at the same time. The results were recorded according to the skin reaction grading standard of the skin occlusion patch test in Chapter 7 of the "Technical Specifications for Safety of Cosmetics" (2015 edition). The safety patch test results are shown in Table 6.

[0085] Table 6

[0086]

[0087] The results of human skin patch tests showed that among 35 subjects, no adverse skin reactions occurred, indicating that the black propolis tea extract composition prepared by the present invention is highly safe.

[0088] Test Example 3: Evaluation of the effect of nourishing the human body

[0089] Test samples: black propolis tea extract compositions prepared in Examples 1-12 and Comparative Examples 1-5.

[0090] Test principle: Refer to T / CAB0152-2022 "Test methods for seven cosmetic efficacy items: anti-wrinkle, firming, moisturizing, oil control, repairing, nourishing, and soothing", adopt the basic method of efficacy testing on human subjects, and use the data results of objective indicators of the test area before and after D0 and D28 of the test product to determine whether the test product has a nourishing effect.

[0091] Test instruments: multifunctional skin tester (Antsci Skin-FX), skin colorimetry tester (ANTSCICI6X), VISIA7 image camera, skin texture tester (VC98).

[0092] Test process:

[0093] 1. Volunteer subjects were recruited as required, with 32 subjects in each group, aged 25-55 years old. After washing their faces with water, the subjects sat quietly in a constant temperature and humidity chamber for 30 minutes. The water content of the stratum corneum, skin gloss, roughness, and smoothness were measured and recorded, and the test data was recorded as the initial value D0.

[0094] 2. After the test is completed, the product and product usage instructions will be distributed. The subjects will use the test product for 28 consecutive days. During this period, they must record the usage and changes in skin condition in a standardized manner.

[0095] 3. On-site return visit, sit in a constant temperature and humidity chamber for 30 minutes, and then conduct the test. The test sequence is the same as step 1, and the test data is recorded as the final value D28.

[0096] 4. Calculate the change rate (%) of each item according to formula (1).

[0097] Change rate (%) = (final value D28-initial value D0) / (initial value D0) × 100% - Formula (1)

[0098] Water content of the stratum corneum of the skin: if the water content of the stratum corneum of the skin increases after using the sample, then the product has the effect of increasing the water content of the stratum corneum of the skin.

[0099] Glossiness: If the skin glossiness improves after using the sample, the product has the effect of increasing the skin glossiness.

[0100] Roughness SEr: If the skin roughness SEr value increases after using the sample, the product has the effect of improving skin roughness.

[0101] Smoothness SEsm: If the skin smoothness SEsm value decreases after using the sample, the product has the effect of smoothing the skin.

[0102] Table 7

[0103]

[0104]

[0105] The test results are shown in Table 7. The black propolis tea extract composition prepared by the present invention has the effect of significantly increasing the water content of the stratum corneum and improving the roughness, and also has the effect of increasing the glossiness and improving the smoothness, that is, it has a good nourishing effect. Among them, the black propolis tea extract composition of Example 10 has the best effect, that is, the best ratio of the black propolis tea extract composition is 0.2 parts of EGCG, 5 parts of royal jelly extract, and 5 parts of black propolis extract.

[0106] Compared with Example 10, the various indicators of the nourishing effect of the black propolis tea extract composition of Comparative Examples 1-5 have significantly decreased, indicating that the black propolis tea extract composition lacks any active component, or the proportion of each component exceeds the scope of the present invention, or the replacement of a certain component with a similar component cannot achieve the good synergistic effect of the present invention. Among them, the replacement of royal jelly extract with ordinary honey extract in Comparative Example 5 will also cause a decrease in effect. The reason is that the sources of honey extract and royal jelly extract are different. Honey is brewed by bees collecting nectar, and royal jelly is a special milky substance secreted by worker bees for feeding queen bees and larvae. The ingredients contained in the two are also different. The main components of honey extract are glucose, fructose, sucrose, amino acids, vitamins, minerals, enzymes, organic acids, etc. The components of royal jelly extract mainly include protein, amino acids (including special royal jelly acid), vitamins, minerals, fatty acids and enzymes. The ingredients in royal jelly extract are more complex, and the various nutrients it contains can be directly absorbed by the human body. Therefore, when combined with other components, it can work synergistically to the greatest extent to exert the nourishing, skin-beautifying, and anti-aging effects of the combination, thereby improving the nutritional value and efficacy of the product.

[0107] Test Example 4: Elastase Test

[0108] Experimental principle: Collagen forms collagen fibers and reticular fibers, and elastin forms elastic fibers. These three fibers together form the connective tissue of the skin, which makes the skin plump and elastic. Elastase has the ability to decompose collagen, elastin and other proteins. The change in elastin structure is closely related to skin aging. The inhibitory effect of the test sample on the catalysis of elastase and its substrate is detected by an ELISA instrument to determine whether the test sample has the ability to inhibit elastase.

[0109] 1. Reagents and materials: 30 U / mg elastase (porcine pancreas), N-succinyl-alanine-alanine-alanine-p-nitroaniline, tris(hydroxymethyl)aminomethane, concentrated hydrochloric acid, EGCG.

[0110] 2. Instruments and equipment: analytical balance (accurate to 0.001g), ultrasonic cleaning machine, pipette, pipette, colorimetric tube, volumetric flask (10mL, 100mL).

[0111] 3. Determination steps:

[0112] (3.1) Solution preparation:

[0113] (3.1.1) Preparation of 0.2 mol / L Tris-HCl buffer at pH 8.0:

[0114] Solution A: Accurately weigh 4.8456 g of Tris particles, add 100 mL of deionized water, stir and dissolve to obtain a 0.4 mol / L Tris solution;

[0115] Solution B: Take 3.34 mL of concentrated hydrochloric acid and add 96.66 mL of deionized water to dilute to obtain a 0.4 mol / L HCl solution;

[0116] Take 100 mL of liquid A and 44.76 mL of liquid B, mix them, adjust the pH to 8.0, and add deionized water to make up to 200 mL.

[0117] (3.1.2) Elastase solution: Prepare with pH 8.0 Tris-HCL buffer. Take 0.033 g of elastase and add 10 mL of pH 8.0 Tris-HCL buffer to prepare a 100 U / mL elastase solution. Prepare it immediately before use.

[0118] (3.1.3) Substrate solution: Weigh 1.8057 mg of N-succinyl-alanine-alanine-alanine-p-nitroaniline and dissolve it in 10 mL of pH 8.0 Tris-HCl buffer.

[0119] (3.1.4) Positive control EGCG solution: Weigh 0.001 g of EGCG powder and dissolve it in 1 mL of pH 8.0 Tris-HCl buffer to prepare a 1.0 mg / mL solution, which was then diluted with the buffer to prepare solutions of 0.5, 0.1, 0.01, 0.005, 0.003, 0.002, and 0.001 mg / mL.

[0120] (3.1.5) Sample solution: the black propolis tea extract composition solution of Examples 1-12 and Comparative Examples 1-5.

[0121] 4. Prepare the test solution:

[0122] Table 8

[0123]

[0124] According to Table 8, each solution was added to a 96-well plate in sequence, incubated at room temperature for 60 min, and the absorbance was detected at 410 nm using an ELISA reader. The data was recorded and saved, and the elastase inhibition rate (%) was calculated according to formula (2).

[0125] Elastase inhibition rate (%) = [1-(ODA -OD B ) / (OD C -OD D )]×100%——Formula (2)

[0126] In the above formula, OD A is the absorbance value of the sample group (containing enzyme and substrate);

[0127] OD B is the absorbance value of the sample control group (containing enzyme but not substrate);

[0128] OD C is the absorbance value of the blank group (containing enzyme, substrate and no sample);

[0129] OD D It is the absorbance value of the blank control group (containing enzyme but no substrate or sample).

[0130] 5. Precision: The absolute difference between two independent measurement results obtained under repeatability conditions shall not exceed 10% of the arithmetic mean.

[0131] Table 9

[0132]

[0133]

[0134] The test results are shown in Table 9. The black propolis tea extract composition prepared by the present invention has significant inhibition of elastase, that is, has good anti-wrinkle effect. Among them, the black propolis tea extract composition of Example 10 has the best effect, that is, the best ratio of the black propolis tea extract composition is 0.2 parts of EGCG, 5 parts of royal jelly extract, and 5 parts of black propolis extract.

[0135] Compared with Example 10, the elastase inhibition rates of the black propolis tea extract compositions of Comparative Examples 1-5 were significantly reduced, that is, the anti-aging effects were significantly reduced, indicating that the black propolis tea extract compositions lacked any active ingredient, or the proportions of the components exceeded the scope of the present invention, or replacing a component with a similar component could not achieve the good synergistic effect of the present invention.

[0136] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A black propolis tea extract composition, characterized in that: The invention comprises the following components in parts by weight: 0.001-0.2 parts of EGCG, 1-5 parts of royal jelly extract and 0.5-5 parts of black propolis extract.

2. The black propolis tea extract composition according to claim 1, characterized in that The invention comprises the following components in parts by weight: 0.05-0.2 parts of EGCG, 1-5 parts of royal jelly extract and 1-5 parts of black propolis extract.

3. The black propolis tea extract composition according to claim 2, characterized in that: The invention comprises the following components in parts by weight: 0.2 parts of EGCG, 5 parts of royal jelly extract and 5 parts of black propolis extract.

4. Use of the black propolis tea extract composition according to any one of claims 1 to 3 in cosmetics.

5. A cosmetic, characterized in that: It comprises the black propolis tea extract composition as described in any one of claims 1 to 3.

6. The cosmetic according to claim 5, characterized in that The black propolis tea extract composition is added in an amount of 1.501%-10.2% in cosmetics.

7. The cosmetic according to claim 6, characterized in that The addition amount of the black propolis tea extract composition in cosmetics is 10.2%.

8. The cosmetic according to claim 5, characterized in that The dosage forms of the cosmetics include water, milk and cream.

9. The cosmetic according to claim 5, characterized in that: The cosmetics also include auxiliary materials acceptable in daily chemical products.

10. The cosmetic according to claim 9, characterized in that The auxiliary materials include at least one of an emollient, a dispersant, a humectant, a preservative, an emulsion stabilizer, a thickener, and a pH regulator.

Images