Animal fat extraction method and device

Abstract

The invention relates to an animal fat extraction method and device and relates to the technical field of fat extraction.The animal fat extraction method comprises the steps of pretreatment, enzymolysis, water removal and separation.By means of the animal fat extraction method and device, it can be effectively avoided that the activity of animal fat compound enzyme is reduced after enzymolysis is conducted for a period of time, and the animal fat extraction efficiency is improved. The grease yield is improved, the oxidation of the grease can be delayed, and the grease is not easy to deteriorate.

Description

Technical Field

[0001] The invention relates to the technical field of oil extraction, and in particular to a method and device for extracting animal oil. Background Art

[0002] With the continuous progress of human society, the demand for oil resources continues to rise. Animal fats and oils are a natural and renewable resource. After refining, they have a wide range of applications and significant value. In the food processing industry, animal fats and oils can not only be used as high-quality raw materials for edible oils and baking oils, but also add unique flavors and nutritional value to food.

[0003] The Chinese invention patent application with publication number CN107474935A and publication date December 15, 2017, proposes an animal fat and an extraction process thereof, which discloses an animal fat and an extraction process thereof, comprising the following steps: (1) putting pig fat tissue and pig skin into a reactor under stirring conditions, heating the temperature to 55-60°C, and keeping the temperature for 3-5 hours; (2) heating the temperature to 85-90°C, and keeping the temperature for 1-3 hours; (3) adding pork enzymatic hydrolyzate powder, heating the temperature to 97-103°C, and keeping the temperature for 1-3 hours; 3 hours; (4) cool to 85-95°C, add water, and keep warm for 3-5 hours; (5) cool to 55-60°C, separate and obtain oily material; (6) add ingredients, stir evenly, heat to 60-65°C, and stop stirring and heating for 0.5-1 hour; (7) heat to 95-100°C, and keep warm for 1-3 hours; (8) cool to 60-65°C, add fragrance base; (9) cool to 40-45°C, stir evenly, and separate and obtain ointment; (10) grind the ointment with a colloid mill to obtain animal fat.

[0004] The low-temperature extraction process produces less oil smoke and no toxic substances, and the extracted animal fat has the advantages of uniformity, good taste and good color.

[0005] With respect to the above technical solutions, methods for improving the esterification efficiency of oils and fats, reducing the acid value, and improving the quality of oils and fats still need to be discovered. Summary of the invention

[0006] In order to improve the esterification efficiency of oil, reduce the acid value and improve the quality of oil, the present invention provides an animal oil extraction method and device.

[0007] In a first aspect, the present invention provides a method for extracting animal fats, comprising the following components and their weight proportions: 1-6 parts of protease, 1-6 parts of lipase and 0.5-7 parts of sodium citrate.

[0008] By adopting the above technical solution, protease is used to break up fat cells. Protease can effectively enzymatically decompose the protein structure on the cell membrane structure, destroy the cell membrane of fat cells, and allow the oil to precipitate automatically. The fatty acids in crude oil are converted into glycerides by lipase catalysis. This process connects the fatty acids back to the glyceride backbone, which reduces the acid value and increases the oil yield. At this time, the addition of sodium citrate keeps the system in a stable pH range of 7-8, which can effectively prevent the protease activity from being greatly reduced after a period of enzymatic hydrolysis during protein hydrolysis, and the oil will bring in trace amounts of metal ions during the refining and deodorization process. These metal ions can promote the oxidation and rancidity of the oil, which will have an adverse effect on the storage and transportation of the oil and cause the oil to have an odor. Sodium citrate can chelate with metal ions, effectively reducing the inactivation of enzymes caused by metal ions brought by the enzymatic hydrolysis of animal fat tissue during the enzymatic hydrolysis process, and will not make the enzymatic hydrolysis rate too low, effectively avoiding oil emulsification, which is beneficial to the separation of oil. The addition of sodium citrate can also act as an acyl acceptor. The hydroxyl group in its structure can undergo esterification reaction with the carboxyl group. Sodium citrate reacts with free glycerol and free fatty acids to generate citric acid glycerol fatty acid ester. Citric acid glycerol fatty acid ester is easily soluble in oils and fats. Citric acid glycerol fatty acid ester can chelate trace metal ions in oils and fats, and can delay the oxidation of oils and fats. At the same time, it can be used to stabilize the emulsified system containing fat, so that the oil droplets are not easy to aggregate and stratify, and are not easy to deteriorate. The oil obtained by esterification and deacidification has a stronger emulsification stability than conventional refined oils with the same emulsifier added. It can be decomposed into fatty acids and polyols during human digestion, and then absorbed or excreted from the body. It has no adverse effect on human metabolism and does not accumulate in the human body to affect health.

[0009] Optionally, the protease is alkaline protease.

[0010] By adopting the above technical solution, the system is in a stable pH value range of 7-8. The alkaline protease is selected to act on the protein action sites in animal fats to the maximum extent, hydrolyzing the proteins in the scraps that are difficult to digest by the endogenous animal proteases into peptides and amino acids, destroying the close combination of protein and fat and releasing the fat.

[0011] Optionally, the lipase is at least one of Candida antarctica lipase, Geobacillus lipase, Thermomyces thermophilus, Pseudomonas fluorescens lipase, Pseudomonas aeruginosa lipase, Talaromyces thermophilus lipase and Thermomyces lanuginosus lipase.

[0012] By adopting the above technical solution, the alkali-resistant thermophilic lipase is selected to provide higher lipase activity in the pH range of 7-8.5 and at a higher enzymatic hydrolysis temperature.

[0013] In a second aspect, the present invention provides a method for extracting animal fat, comprising the following technical solutions: Octadecyl Sepabeads, protease and lipase are added to a 10 mM sodium phosphate solution, wherein the mass of the protease accounts for 1-2 wt % of the mass of the octadecyl Sepabeads, and the mass of the lipase accounts for 1-2 wt % of the mass of the octadecyl Sepabeads. After stirring at a speed of 250 rpm for 7 hours, the suspension is filtered and washed with distilled water for multiple times to obtain an immobilized enzymatic hydrolysis composition.

[0014] By adopting the above technical scheme, the immobilization rates of protease and lipase are both higher than 95%. By immobilizing two different forms of enzymes in a one-step method, a step of different enzymes being immobilized in different ways can be saved. At the same time, the immobilized enzymes immobilized in the same way can be removed in the same way without having to be removed separately multiple times. The enzymes interact with the hydrophobic carrier octadecyl Sepabeads. These immobilized lipases and proteases are single protease and lipase molecules in an open form. Due to the interfacial activation of the enzyme after immobilization, the enzyme activity is further increased. The adsorbed enzyme is in an open form, and the active center is available for substrate contact, so that the enzyme's efficacy can be more thoroughly exerted. At the same time, due to the open structure, the enzyme is adsorbed to the hydrophobic interface through the very hydrophobic area formed on the inner surface of the valve and around the active center. This adsorption promotes significant changes in the enzyme structure, resulting in a significantly more ordered enzyme structure, increasing the stability of the immobilized enzyme, making it less likely to be inactivated, and still having high activity after repeated use.

[0015] At the same time, octadecyl Sepabeads are hydrophobic carriers that can keep lipase away from water molecules in the system, which is beneficial for the reaction to proceed in the direction of esterification, so that the esterification products citric acid glycerol fatty acid esters and glycerides are generated more fully.

[0016] In a third aspect, the present invention provides a method for extracting animal fat, comprising the following steps: Pre-treatment: remove impurities from the animal fat tissue, clean it, crush it, and put it into a container; Adding enzyme: adding the animal fat compound enzyme described in the first aspect and water into the above container, wherein the mass of the animal fat compound enzyme accounts for 1-4wt% of the mass of water, and the mass of water accounts for 1-3wt% of the mass of animal fat tissue, and stirring evenly to obtain a mixture of fat and enzyme; Enzymolysis: The mixture of oil and enzyme is enzymolyzed at 50-68℃ for 10-20min to obtain an enzymolysis mixture. Dehydration: add the enzymatic mixture to a super absorbent resin, perform enzymatic hydrolysis at 50-68°C for 60-480 min, then heat to 90-100°C to inactivate the enzyme, and use a filter to intercept and separate the super absorbent resin, wherein the filter has an aperture of 0.1 mm-1 mm, to obtain a grease mixture; Separation: Centrifuge the oil mixture at 5000-8000r / min for 15 minutes to obtain animal oil.

[0017] By adopting the above technical scheme, the animal fat is first pre-treated and purified, which is conducive to obtaining purer animal fat, and adding animal fat compound enzyme to the pre-treated animal fat for enzymatic hydrolysis reaction is conducive to the precipitation of fat. Since the esterification reaction is a reversible reaction, water is generated in the reaction. Timely removal of the water generated in the reaction is conducive to the reaction in the direction of esterification. By vacuum heating in a rotary evaporator, the evaporation temperature of water can be lowered, and it can evaporate at low temperatures, effectively removing water in the reaction process and making the esterification products citric acid glycerol fatty acid ester and glyceride more fully generated.

[0018] In a fourth aspect, the present invention provides a method for extracting animal fat, comprising the following steps: Pre-treatment: remove impurities from the animal fat tissue, clean it, chop it up, and put it into a container; Adding enzyme: adding the animal fat composite enzyme of the first aspect solidified by the solidification method of the second aspect and water into the container, wherein the mass of the immobilized enzymatic hydrolysis composition accounts for 10-40wt% of the mass of water, and the mass of the water accounts for 1-3wt% of the mass of the animal fat tissue, and stirring evenly to obtain a mixture of fat and enzyme; Enzymolysis: The mixture of oil and enzyme is enzymolyzed at 50-68℃ for 10-480min to obtain an enzymolysis mixture. Enzyme removal: The enzymatic mixture is intercepted and separated by a filter screen, wherein the pore size of the filter screen is 0.1 mm-1 mm, to obtain a grease mixture.

[0019] Separation: Centrifuge the oil mixture at 5000-8000r / min for 15 minutes to obtain animal oil.

[0020] By adopting the above technical scheme, the animal fat is first pre-treated and purified, which is conducive to obtaining purer animal fat, and adding animal fat compound enzyme to the pre-treated animal fat for enzymatic hydrolysis reaction is conducive to the precipitation of fat. Enzyme removal can separate and recover the immobilized enzymatic hydrolysis composition for reuse.

[0021] Optionally, there is an electrolysis step after the enzyme removal step.

[0022] Electrolysis: The oil intercepted and separated by the immobilized enzymatic hydrolysis composition is treated by a low-voltage direct current electric field to obtain an oil mixture. The voltage range of the low-voltage direct current electric field can be 5-75V, and the current range is 0-3A.

[0023] By adopting the above technical scheme, the undecomposed animal fat can be further decomposed by an external electric field. Under low pressure, the active protein in the animal raw material will not lose its activity due to the influence of high temperature and high pressure. At the same time, the solution is maintained in the pH range of 7-8.5 to provide an alkaline environment, which provides a more suitable electrolysis environment for electrolysis and improves the electrolysis efficiency.

[0024] Optionally, the electrolysis temperature of the electrolysis is maintained at 50-60°C.

[0025] By adopting the above technical solution, increasing the temperature while applying the electric field helps to improve the oil extraction rate, while the active fats and active proteins will not be inactivated due to excessively high temperatures.

[0026] Optionally, the electrolysis step is stirred using a stirrer.

[0027] By adopting the above technical solution, the processing effect of the low-voltage direct current electric field is enhanced, the reaction is made more uniform and sufficient, and the reaction time is shortened.

[0028] In a fifth aspect, the present invention provides an animal fat extraction device, comprising a tank body, a tank cover, an electric push cylinder, an electric hydraulic push rod, an oil outlet, a heating mechanism, a stirring mechanism and a filtering mechanism. The heating mechanism comprises a heating shell and a heating tube, wherein the heating shell is sleeved on the tank body, a heating cavity is formed between the heating shell and the tank body, a heating medium is filled in the heating cavity, the heating medium is oil or water, and the heating tube is placed in the heating cavity; The stirring mechanism comprises a stirring motor and a stirring paddle, the stirring paddle is rotatably arranged in the tank body, and the stirring motor is transmission-connected to the stirring paddle; The filtering mechanism includes a support frame, a filter screen and a filter screen, wherein the filter screen and the filter screen are arranged on the support frame, the support frame is arranged in the tank body, the filter screen is arranged on the upper part of the filter screen, and the filter screen is arranged on the upper part of the stirring paddle.

[0029] By adopting the above technical scheme, an electric push cylinder and an electric hydraulic push rod are used to open the tank cover, animal fat tissue is placed in the tank body and placed on the filter screen, the animal fat complex enzyme or the immobilized enzymatic hydrolysis composition is added to dissolve the fat, and the super absorbent resin or the immobilized enzymatic hydrolysis composition can be filtered out by the filtering mechanism for recovery, the heating mechanism makes the device reach a preset temperature, and the stirring mechanism makes the fat and enzyme mix evenly, which can better improve the enzymatic hydrolysis efficiency, and finally the oil flows out from the oil outlet for collection.

[0030] Optionally, a plurality of filter screens are provided, and the plurality of filter screens are evenly distributed along their normal directions, and the mesh apertures of the filter screens gradually decrease from the direction away from the stirring paddle to the direction close to the stirring paddle.

[0031] By adopting the above technical solution, the immobilized enzymatic hydrolysis composition can be filtered more completely.

[0032] Optionally, an electrolysis device is further provided in the tank body, and the electrolysis device comprises a positive electrode and a negative electrode, and the positive electrode and the negative electrode are respectively provided on both sides of the axis of the tank body.

[0033] By adopting the above technical solution, electrolysis can be carried out directly in the tank body.

[0034] In summary, the present invention includes at least one of the following beneficial technical effects: The present invention utilizes protease to break fat cells. At this time, the addition of sodium citrate makes the system in a stable pH value range of 7-8, which can effectively avoid that the activity of the protease is greatly reduced after a period of enzymatic hydrolysis during the protein hydrolysis process. It can chelate with metal ions, effectively reduce the inactivation effect of the enzyme caused by metal ions after the enzymatic hydrolysis of animal fat tissue during the enzymatic hydrolysis process, and will not make the enzymatic hydrolysis rate too low, effectively avoid oil emulsification, and thus be beneficial to the separation of oil.

[0035] The present invention utilizes lipase catalytic reaction to convert fatty acids in crude oil into glycerides, thereby improving the yield of oil. The addition of sodium citrate can also serve as an acyl acceptor. The hydroxyl group contained in the structure can undergo esterification reaction with the carboxyl group. The generated citric acid glycerol fatty acid ester can chelate trace metal ions in the oil and grease, and can delay the oxidation of the oil and grease. At the same time, it can be used to stabilize the emulsified system containing fat, so that the oil droplets are not easy to aggregate and stratify, and are not easy to deteriorate.

[0036] The immobilization rates of the immobilized protease and lipase of the present invention are both higher than 95%. By using a one-step method, two enzymes in different forms are immobilized, which can save a step of using different enzymes in different immobilization methods. At the same time, the immobilized enzymes immobilized in the same way can be removed using the same method without the need for multiple separate removals. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] Figure 1 It is a schematic diagram of the animal fat extraction device of the present invention; Figure 2 It is a partial cross-sectional view of the animal fat extraction device of the present invention.

[0038] Explanation of the accompanying drawings: 1. tank body; 2. tank cover; 3. stirring motor; 4. electric push cylinder; 5. positive electrode; 6. negative electrode; 7. electric hydraulic push rod; 8. filter placement; 9. filter; 10. stirring paddle; 11. heating shell; 12. heating tube; 13. oil outlet. DETAILED DESCRIPTION

[0039] The present invention is further described in detail below with reference to the embodiments.

[0040] Example 1: This example discloses an animal fat complex enzyme, comprising the following components and their weight proportions: 2 parts of alkaline protease, 2 parts of thermophilic bacillus lipase and 4 parts of sodium citrate.

[0041] This embodiment discloses a method for extracting animal fat, comprising the following steps: Pre-treatment: remove impurities from the animal fat tissue, clean it, crush it, and put it into the animal fat extraction device; Adding enzyme: adding the above-mentioned animal fat compound enzyme and water to the above-mentioned animal fat extraction device, wherein the mass of the animal fat compound enzyme accounts for 2wt% of the mass of water, and the mass of water accounts for 2.5wt% of the mass of animal fat tissue, and stirring evenly to obtain a mixture of fat and enzyme; Enzymolysis: The mixture of oil and enzyme was enzymolyzed at 58℃ for 20min to obtain an enzymolysis mixture. Dehydration: add the enzymatic mixture to the super absorbent resin, enzymatically hydrolyze at 58°C for 140 minutes, then heat to 90-100°C to inactivate the enzyme, and use a filter to intercept and separate the super absorbent resin, the filter pore size of which is 0.1mm-1mm to obtain a grease mixture; Separation: Centrifuge the oil mixture at 6000r / min for 15min to obtain animal oil.

[0042] This embodiment discloses an animal fat extraction device, comprising a tank body 1, a tank cover 2, an electric push cylinder 4, an electric hydraulic push rod 7, an oil outlet 13, a heating mechanism, a stirring mechanism and a filtering mechanism. The heating mechanism comprises a heating shell 11 and a heating tube 12. The heating shell 11 is sleeved on the tank body 1. A heating cavity is formed between the heating shell 11 and the tank body 1. A heating medium is filled in the heating cavity. The heating medium is oil or water. The heating tube 12 is placed in the heating cavity. The stirring mechanism comprises a stirring motor 3 and a stirring paddle 10, the stirring paddle 10 is rotatably arranged in the tank body 1, and the stirring motor 3 is transmission-connected to the stirring paddle 10; The filtering mechanism includes a support frame, a filter screen 8 and a filter screen 9, wherein the filter screen 8 and the filter screen 9 are arranged on the support frame, the support frame is arranged in the tank body 1, the filter screen 8 is arranged on the upper part of the filter screen 9, and the filter screen 9 is arranged on the upper part of the stirring paddle 10.

[0043] The filter screens 9 are provided in plurality and are evenly distributed along their normal directions. From the direction away from the stirring paddle 10 to the direction close to the stirring paddle 10 , the mesh apertures of the filter screens 9 gradually decrease.

[0044] When extracting animal fat, the impurity-removed, washed and chewed animal fat tissue is placed on the filter 8, and then the animal fat compound enzyme and water are added to the tank body 1. The heating medium in the heating chamber is heated by the heating tube 12 to maintain the temperature in the tank body 1 at 58°C. At the same time, the stirring mechanism stirs the material in the tank body 1 for enzymolysis, and then a highly absorbent resin is added into the tank body 1 to absorb excess water and continue the enzymolysis. After the enzymolysis is completed, the heating tube 12 is heated to inactivate the animal fat compound enzyme, and then the highly absorbent resin is filtered and recovered to obtain a fat mixture.

[0045] Example 2: This example discloses an animal fat complex enzyme, comprising the following components and their weight proportions are the same as those in Example 1, The animal fat compound enzyme is a modified animal fat compound enzyme, Modification method: octadecyl Sepabeads, alkaline protease and thermophilic Bacillus lipase are added to a 10 mM sodium phosphate solution, the mass of the alkaline protease accounts for 1wt% of the mass of the octadecyl Sepabeads, and the mass of the thermophilic Bacillus lipase accounts for 1wt% of the mass of the octadecyl Sepabeads. After stirring at a speed of 250 rpm for 7 hours, the suspension is filtered and washed with distilled water for multiple times to obtain an immobilized enzymatic hydrolysis composition.

[0046] This embodiment discloses a method for extracting animal fat, comprising the following steps: Adding enzyme: adding the above-mentioned immobilized enzymatic hydrolysis composition and water to the above-mentioned animal fat extraction device, wherein the mass of the immobilized enzymatic hydrolysis composition accounts for 20wt% of the mass of water, and the mass of water accounts for 2.5wt% of the mass of animal fat tissue, and stirring evenly to obtain a mixture of fat and enzyme; Enzymolysis: The mixture of oil and enzyme was enzymolyzed at 58°C for 160 minutes to obtain an enzymolysis mixture. A mildew removal step is used to replace the water removal step, and enzyme removal: the enzymatic hydrolysis mixture is intercepted and separated by a filter screen with an aperture of 0.1 mm to obtain a grease mixture.

[0047] The rest is the same as in Example 1. This embodiment discloses an animal fat extraction device, which has the same structure as that of embodiment 1, except that it does not require inactivation and water removal treatment during use, and the immobilized enzymatic hydrolysis composition can be directly filtered and recovered, and the immobilized enzymatic hydrolysis composition can be reused.

[0048] Example 3: This example discloses a method for extracting animal fat, comprising the following steps.

[0049] This embodiment discloses a method for extracting animal fat, comprising the following steps: Adding an electrolysis step after the enzyme removal step, Electrolysis: The oil intercepted and separated by the immobilized enzymatic hydrolysis composition is treated by a low-voltage direct current electric field. The electrolysis time is 50 minutes and the electrolysis temperature is 58°C. During the electrolysis, the oil is stirred by a stirrer to obtain an oil mixture. The voltage of the low-voltage direct current electric field is 15V. The electrolysis time is adjusted according to the electric field strength of the low-voltage direct current electric field. The smaller the voltage, the longer the electrolysis time.

[0050] The rest is consistent with Example 2.

[0051] This embodiment discloses an animal fat extraction device. An electrolysis device is further arranged in the tank body 1. The electrolysis device comprises a positive electrode 5 and a negative electrode 6. The positive electrode 5 and the negative electrode 6 are arranged on both sides of the axis of the tank body 1 respectively.

[0052] The rest is consistent with Example 2.

[0053] Example 4: This example discloses a method for extracting animal fat, comprising the following steps.

[0054] Adding enzyme: The added enzyme is the immobilized enzymatic hydrolysis composition filtered out in the enzyme removal step.

[0055] The rest is consistent with Example 2.

[0056] Comparative Example 1: This comparative example discloses an animal fat complex enzyme, comprising the following components and their weight proportions: 2 parts alkaline protease and 2 parts sodium citrate.

[0057] This comparative example discloses a method for extracting animal fat, comprising the following steps: Pre-treatment: remove impurities from the animal fat tissue, clean it, chop it up, and put it into a container; Adding enzyme: adding the above-mentioned animal fat compound enzyme and water into the above-mentioned container, wherein the mass of the animal fat compound enzyme accounts for 2wt% of the mass of water, and the mass of water accounts for 2.5wt% of the mass of animal fat tissue, and stirring evenly to obtain a mixture A of fat and enzyme; Enzymolysis: The mixture of oil and enzyme is enzymolyzed at 58°C for 140 min, then heated to 95°C to inactivate the enzyme, to obtain an oil mixture; Separation: Centrifuge the oil mixture at 6000r / min for 15min to obtain animal oil.

[0058] Comparative Example 2: This comparative example discloses an animal fat complex enzyme, comprising the following components and their weight proportions: 2 parts of thermophilic bacillus lipase and 2 parts of sodium citrate.

[0059] This comparative example discloses a method for extracting animal fat, comprising the following steps: Pre-treatment: remove impurities from the animal fat tissue, clean it, crush it, and put it into a container; Adding enzyme: adding the above-mentioned animal fat compound enzyme into the above-mentioned container, the mass of the animal fat compound enzyme accounts for 0.5wt‰ of the mass of the animal fat tissue, and stirring evenly to obtain a mixture of fat and enzyme; Enzymolysis: The mixture of oil and enzyme is enzymolyzed at 58°C for 140 min, then heated to 95°C to inactivate the enzyme, to obtain an oil mixture; Separation: Centrifuge the oil mixture at 6000r / min for 15min to obtain animal oil.

[0060]

[0061] By comparing Example 1, Example 2 and Example 4, it can be seen that by immobilizing the protease and the lipase, the activity of the protease and the lipase can be enhanced, the oil yield is increased, the acid value and the peroxide value are reduced, which is conducive to obtaining high-quality oils and higher oxidative stability. At the same time, the immobilized enzymatic hydrolysis composition is reused without obvious reduction in its performance, indicating that this immobilization method can be applied to the two enzymes and can be reused, effectively reducing the consumption of the enzyme.

[0062] From the comparison between Example 2 and Example 3, it can be seen that the efficiency of oil extraction can be increased by electrolysis, while the active fats and active proteins will not be inactivated due to excessively high temperatures, thereby reducing the quality of animal fats.

[0063] By comparing Example 1, Comparative Example 1 and Comparative Example 2, it can be seen that the co-addition of two enzymes is more conducive to improving the oil extraction efficiency and quality of animals than adding a single enzyme.

[0064] The above are all preferred embodiments of the present invention, and are not intended to limit the protection scope of the present invention. Therefore, any equivalent changes made based on the structure, shape, and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for extracting animal fat, characterized in that: The following steps are involved: Pre-treatment: remove impurities from the animal fat tissue, clean it, crush it, and put it into a container; Adding enzyme: Add animal fat compound enzyme and water to the above container, wherein the mass of the animal fat compound enzyme accounts for 1-4wt% of the mass of water, and the mass of water accounts for 1-3wt% of the mass of animal fat tissue, and stir evenly to obtain a mixture of fat and enzyme; the animal fat compound enzyme includes the following components and their weight proportions: 1-6 parts of protease, 1-6 parts of lipase and 0.5-7 parts of sodium citrate; Enzymolysis: The mixture of oil and enzyme is enzymolyzed at 50-68℃ for 10-480min to obtain an enzymolysis mixture. Dehydration: add the enzymatic mixture to a super absorbent resin, perform enzymatic hydrolysis at 50-68°C for 60-480 min, then heat to 90-100°C to inactivate the enzyme, and use a filter to intercept and separate the super absorbent resin, wherein the filter has an aperture of 0.1 mm-1 mm, to obtain a grease mixture; Separation: Centrifuge the oil mixture at 5000-8000r / min for 15 minutes to obtain animal oil.

2. The method for extracting animal fat according to claim 1, characterized in that: The protease is alkaline protease.

3. The method for extracting animal fat according to claim 1, characterized in that: The lipase is at least one of antarctic Candida lipase, Geobacillus lipase, thermophilic Bacillus, Pseudomonas fluorescens lipase, Pseudomonas aeruginosa lipase, thermophilic Talaromyces lipase and Thermomyces lanuginosus lipase.

4. The method for extracting animal fat according to claim 1, characterized in that: The animal fat complex enzyme is solidified through a solidification step, Solidification: octadecyl Sepabeads, protease and lipase are added to a 10 mM sodium phosphate solution, wherein the mass of the protease accounts for 1-2 wt % of the mass of the octadecyl Sepabeads, and the mass of the lipase accounts for 1-2 wt % of the mass of the octadecyl Sepabeads. After stirring at a speed of 250 rpm for 7 hours, the suspension is filtered and washed with distilled water several times to obtain an immobilized enzymatic hydrolysis composition. The dehydration step is replaced by an enzyme removal step. For enzyme removal, the enzymatic mixture is intercepted and separated by a filter screen to separate the immobilized enzymatic composition, wherein the filter screen has an aperture of 0.1 mm to 1 mm, to obtain a grease mixture.

5. The method for extracting animal fat according to claim 4, characterized in that: The enzyme removal step is followed by an electrolysis step. Electrolysis: The oil intercepted and separated by the immobilized enzymatic hydrolysis composition is treated by a low-voltage direct current electric field to obtain an oil mixture. The voltage range of the low-voltage direct current electric field can be 5-75V, and the current range is 0-3A.

6. The method for extracting animal fat according to claim 4, characterized in that: The electrolysis temperature of the electrolyte is maintained at 50-60°C.

7. The method for extracting animal fat according to claim 4, characterized in that: The electrolysis step is stirred using a stirrer.

8. An animal fat extraction device, used to implement the animal fat extraction method according to any one of claims 1 to 7, comprising a tank body (1), a tank cover (2), an electric push cylinder (4), an electric hydraulic push rod (7), an oil outlet (13), a heating mechanism, a stirring mechanism and a filtering mechanism, The heating mechanism comprises a heating shell (11) and a heating tube (12); the heating shell (11) is sleeved on the tank body (1); a heating chamber is formed between the heating shell (11) and the tank body (1); a heating medium is filled in the heating chamber; the heating medium is oil or water; and the heating tube (12) is placed in the heating chamber; The stirring mechanism comprises a stirring motor (3) and a stirring paddle (10); the stirring paddle (10) is rotatably arranged in the tank body (1); and the stirring motor (3) is transmission-connected to the stirring paddle (10); The filtering mechanism comprises a support frame, a placement filter screen (8) and a filter screen (9), wherein the placement filter screen (8) and the filter screen (9) are arranged on the support frame, the support frame is arranged in the tank body (1), the placement filter screen (8) is arranged on the upper part of the filter screen (9), and the filter screen (9) is arranged on the upper part of the stirring paddle (10).

9. The animal fat extraction device according to claim 8, characterized in that: A plurality of the filter screens (9) are provided, and the plurality of the filter screens (9) are evenly distributed along their normal direction, and the mesh apertures of the filter screens (9) gradually decrease from the direction away from the stirring paddle (10) to the direction close to the stirring paddle (10).

10. The animal fat extraction device according to claim 8, characterized in that: An electrolysis device is also arranged in the tank body (1), and the electrolysis device comprises a positive electrode (5) and a negative electrode (6), wherein the positive electrode (5) and the negative electrode (6) are respectively arranged on both sides of the axis of the tank body (1).

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