Composition containing arbuscular mycorrhiza and application of composition in prevention and treatment of peanut pests

Abstract

The invention discloses a composition containing arbuscular mycorrhiza and application of the composition to prevention and treatment of peanut pests. The effective components of the composition are luteolin and arbuscular mycorrhizal fungi. The invention provides a composition containing arbuscular mycorrhiza, the composition comprises luteolin and arbuscular mycorrhiza fungi, the luteolin is a natural flavonoid compound and has multiple biological activities of insect resistance, feeding inhibition, oxidation resistance and the like, and by directly influencing digestive systems, behaviors and development of pests, the luteolin can effectively reduce harm of the pests; the arbuscular mycorrhizal fungi are beneficial microorganisms symbiotic with plant root systems, the arbuscular mycorrhizal fungi and the arbuscular mycorrhizal fungi are compounded, the arbuscular mycorrhizal fungi can help peanuts to better absorb nutrients, so that the stress resistance of plants is improved, the peanuts have better resistance when being invaded by pests, luteolin directly inhibits the pests, and the yield of the peanuts is increased. The combination of the two can effectively improve the durability of the insecticidal effect and reduce the sensitivity to repeated occurrence of pests.

Description

Technical Field

[0001] The invention relates to the technical field of pest control, and in particular to a composition containing arbuscular mycorrhizae and application of the composition in controlling peanut pests. Background Art

[0002] Peanut, formerly known as groundnut (Arachis hypogaea Linn.), belongs to the Leguminosae family of the Rosaceae order and is an important economic and oil-bearing crop. my country's peanut export trade occupies an important position in the world peanut trade. Peanut production is extremely vulnerable to the threat of many types of pests, resulting in a significant decline in peanut yield and quality. Grubs are a general term for the larvae of the superfamily Scarabaeoidea of ​​the order Coleoptera. They are the most diverse, widely distributed, and most harmful group of underground pests, accounting for more than 80% of the number of underground pests, affecting peanut yield and quality. Aphids are pests with piercing-sucking mouthparts that harm the aboveground parts of plants. Aphids pierce peanut leaves, tender stems, tender shoots, and fruit pods through their mouthparts to suck plant sap. After the plant is sucked, the leaves will curl, turn yellow, or even fall off prematurely, and growth will stagnate.

[0003] At present, chemical pesticides are still the main means of controlling grubs and aphids. Excessive and frequent use of chemical pesticides not only increases the resistance of grubs, but also causes irreversible damage to the ecological environment. Biological control has the advantages of good safety, no pollution to the environment, and long-lasting effect. It has achieved rapid development in recent years. Its research has gradually deepened, and more and more biological control methods have been promoted and applied. Arbuscular mycorrhizal fungi (AMF) belong to the phylum Glomeromycota. They are a type of endophytic fungi that are widely present in soil-borne fungi in agroforestry ecosystems and can coexist with most plants. Luteolin is a naturally occurring plant flavonoid compound that is widely present in many plants, especially some vegetables, herbs and fruits. Compared with conventional chemical pesticides, luteolin is a natural flavonoid compound with lower toxicity to humans and the environment. Therefore, it is considered to be an environmentally friendly insecticide with less impact on non-target organisms (such as beneficial insects such as bees) and humans. Since the mechanism of action of luteolin is different from that of conventional pesticides, its use can effectively reduce the problem of pest resistance caused by the long-term use of conventional pesticides. As an alternative or supplementary natural insecticide, it has good application prospects. At present, there is no report on the combination of luteolin and arbuscular mycorrhizal fungi for the control of peanut pests. Summary of the invention

[0004] In view of the above prior art, the object of the present invention is to provide a composition containing arbuscular mycorrhizae and its application in controlling peanut pests.

[0005] To achieve the above object, the present invention adopts the following technical solution:

[0006] A first aspect of the present invention provides a composition containing arbuscular mycorrhizae, wherein the active ingredients of the composition are luteolin and arbuscular mycorrhizal fungi.

[0007] Preferably, in the composition, the concentration of luteolin is 0.01-0.15 mg / L; and the number of viable bacteria of arbuscular mycorrhizal fungi is ≥1000 spores / mL.

[0008] The second aspect of the present invention provides use of the above composition in controlling peanut pests.

[0009] Preferably, the peanut pests are white grubs or aphids.

[0010] Preferably, the arbuscular mycorrhizal fungus is the arbuscular mycorrhizal fungus Rhizophagus irregularis.

[0011] Preferably, the deposit number of the arbuscular mycorrhizal fungus Rhizophagus irregularis is CCTCC NO: M2014532.

[0012] The third aspect of the present invention provides a method for controlling peanut pests, wherein the composition is applied to the peanuts to be treated by root absorption, spraying or smearing.

[0013] Beneficial effects of the present invention:

[0014] 1. The present invention provides a composition containing arbuscular mycorrhizae, comprising luteolin and arbuscular mycorrhizal fungi. Luteolin is a natural flavonoid compound with multiple biological activities such as insect resistance, feeding inhibition and antioxidant. Luteolin can effectively reduce the harm of pests by directly affecting the digestive system, behavior and development of pests. Arbuscular mycorrhizal fungi are a type of beneficial microorganisms that coexist with plant roots, which can promote the root growth of peanuts and improve the ability of peanuts to absorb water and nutrients, thereby enhancing the resistance and overall health of plants. The two are compounded. The presence of arbuscular mycorrhizal fungi can help peanuts absorb nutrients better, thereby improving the plant's own stress resistance and making peanuts more resistant when attacked by pests. Luteolin directly inhibits pests. The combination of the two can effectively improve the durability of the insecticidal effect and reduce sensitivity to repeated occurrence of pests.

[0015] 2. The main components of the composition containing arbuscular mycorrhizae proposed by the present invention are natural compounds (luteolin) and beneficial microorganisms (arbuscular mycorrhizal fungi), which are relatively low in toxicity and relatively friendly to the environment. Compared with chemical pesticides, this composition can reduce the pollution of chemical agents to the environment and reduce the damage to non-target organisms. DETAILED DESCRIPTION

[0016] It should be noted that the following detailed descriptions are illustrative and are intended to provide further explanation of the present application. Unless otherwise specified, all technical and scientific terms used herein have the same meanings as those commonly understood by those skilled in the art to which the present application belongs.

[0017] In order to enable those skilled in the art to more clearly understand the technical solution of the present application, the technical solution of the present application will be described in detail below in conjunction with specific embodiments.

[0018] The test materials used in the examples of the present invention are all conventional test materials in the art and can be purchased through commercial channels.

[0019] The arbuscular mycorrhizal fungus (AMF) Rhizophagus irregularis CD-1 used in the present invention has a deposit number of CCTCC NO: M2014532, which is recorded in patent CN104450536B, and the AMF agent is prepared by referring to the method of Example 2 of patent CN104450536B.

[0020] Example 1: Preparation of a composition containing arbuscular mycorrhizae:

[0021] The AMF agent, luteolin and deionized water were evenly mixed so that the concentration of luteolin was 0.05 mg / L; the number of viable bacteria of arbuscular mycorrhizal fungi was 1000 spores / mL, and a composition was obtained.

[0022] Comparative Example 1:

[0023] A single AMF inoculant was selected with a viable count of 1000 spores / mL.

[0024] Comparative Example 2:

[0025] A single luteolin solution was selected, wherein the concentration of luteolin was 0.05 mg / L.

[0026] Test Example 1: Anti-insect effect of a composition containing arbuscular mycorrhiza on white grubs

[0027] The peanut variety is Huayu No. 22. After disinfection, the peanut seeds are germinated and whitened. The seeds with the same germination condition are selected and planted in sterilized potting soil. The experiment is divided into two groups:

[0028] Test insect source:

[0029] The grubs were collected from the peanut experimental field of the Plant Protection Experimental Station of Shandong Agricultural University. They were a mixture of dark beetle beetles, large black beetle beetles, and copper green beetle beetles in a ratio of 1:1:1.

[0030] There are 4 treatment groups in the experiment:

[0031] Treatment 1: 10 mL of the composition containing arbuscular mycorrhiza prepared in Example 1 was applied to each peanut plant;

[0032] Treatment 2: 10 mL of the AMF agent of Comparative Example 1 was applied to each peanut plant;

[0033] Treatment 3: 10 mL of the luteolin solution of Comparative Example 2 was applied to each peanut plant;

[0034] In the control group, 10 mL of water was applied to each peanut plant. Then, the soil was irrigated thoroughly with water, and two peanuts were sown in each pot. The peanuts were cultivated in a solar greenhouse, and each group of experiments was repeated three times.

[0035] Peanut rhizomes from different treatment groups were taken to feed grubs, and fresh rhizomes were replaced every day. The growth and development of grubs were observed and recorded. Thirty grubs were selected from each group, and the mortality rate was calculated up to 10 days.

[0036] Experimental results:

[0037] Table 1 Effects of different treatments on grub mortality

[0038] deal with mortality rate(%) Process 1 86.67±5.77a Process 2 13.33±5.09c Process 3 43.33±8.39b Control group 2.22±1.11d

[0039] It can be concluded from Table 1 that the growth and development of peanut rhizomes inoculated with the composition containing arbuscular mycorrhiza prepared in Example 1 were inhibited by grubs, and the insecticidal effect was very significant, reaching more than 85%. Treatments 2 and 3 fed on peanut rhizomes inoculated with a single AMF agent and luteolin solution, respectively, with mortality rates of 12.54% and 43.21%, respectively, and the sum of the grub mortality rates of the two was 55.75%, indicating that the compounding of arbuscular mycorrhizae and luteolin in the present invention has a synergistic effect on improving the insecticidal effect on grubs.

[0040] Example 2: Study on the resistance of a composition containing arbuscular mycorrhiza to peanut grubs in the field:

[0041] The peanut variety was Huayu No. 22, and the field experiment location was the Plant Protection Experiment Station of Shandong Agricultural University, where aphids and white grubs had been causing serious damage over the years.

[0042] Three peanut seeds were sown in each hole, and the experiment was divided into four treatments:

[0043] Treatment 1: 50 mL of the composition containing arbuscular mycorrhiza prepared in Example 1 was added to each hole;

[0044] Treatment 2: 50 mL of the AMF agent of Comparative Example 1 was added to each hole;

[0045] Treatment 3: 50 mL of the luteolin solution of Comparative Example 2 was added to each well;

[0046] In the control group, 50 mL of clean water was added to each hole, covered with soil, and then the entire land was irrigated with water for conventional field management.

[0047] Determination indicators:

[0048] 1. Survey on grub damage:

[0049] The “Z” sampling method was used to survey 5 points in each plot, and 15 holes (1m 2 The entire root system of the peanut plant and the surrounding rhizosphere soil were moved outside the hole, and the number of surviving grubs, the number of peanut pods and the number of damaged pods in each hole and its rhizosphere soil were investigated and recorded, and the pod damage rate was calculated. The results are shown in Table 2.

[0050] 2. Aphid population density:

[0051] During the aphid infestation period, a systematic field survey was conducted every 3 days. A random sampling method was used to select 5 points in each plot, with each point surveying 5m 2 Peanut, the number of aphids at each point was recorded, and the average aphid population density was calculated. The results are shown in Table 3.

[0052] Table 2 Effects of different treatments on grub damage

[0053]

[0054] Table 3 Aphid population density in different treatments

[0055]

[0056]

[0057] The results are shown in Table 2 and Table 3. Compared with the control group, the number of grubs and aphids in Treatment 1 was significantly reduced, indicating that the composition containing arbuscular mycorrhizae of the present invention has a good control effect on grubs and aphids.

[0058] The above description is only the preferred embodiment of the present application and is not intended to limit the present application. For those skilled in the art, the present application may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A composition containing arbuscular mycorrhizae, characterized in that: The effective ingredients of the composition are luteolin and arbuscular mycorrhizal fungi.

2. The composition according to claim 1, characterized in that In the composition, the concentration of luteolin is 0.01-0.15 mg / L; and the number of viable bacteria of arbuscular mycorrhizal fungi is ≥1000 spores / mL.

3. Use of the composition according to claim 1 or 2 in controlling peanut pests.

4. The use according to claim 3, characterized in that: The peanut pests are white grubs or aphids.

5. The use according to claim 3, characterized in that: The arbuscular mycorrhizal fungus is the arbuscular mycorrhizal fungus Rhizophagus irregularis.

6. The use according to claim 5, characterized in that: The deposit number of the arbuscular mycorrhizal fungus Rhizophagus irregularis is CCTCC NO: M2014532.

7. A method for controlling peanut pests, characterized in that: The composition according to claim 1 or 2 is applied to the peanuts to be treated by root absorption, spraying or smearing.

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