One-step enriched cell surface targeting cross-linking agent as well as preparation and application thereof
By introducing trimethylpiperidinyl enrichment units and succinimide ester-sulfonate reactive groups into the crosslinking agent, the problems of low enrichment efficiency and insufficient biocompatibility of existing crosslinking agents are solved, and efficient enrichment and high coverage identification of cell surface proteins are achieved.
Patent Information
- Application Number
- CN202311223550.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2023-09-21
- Publication Date
- 2025-03-21
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
The lack of enrichment groups or the enrichment steps of existing crosslinkers is complicated, resulting in low-abundance cell surface protein identification efficiency and insufficient water solubility and biocompatibility, making it difficult to achieve high coverage identification of live cell surface protein complexes.
A crosslinking agent with trimethylpiperidinyl enrichment unit and succinimide ester-sulfonate reactive group was developed to achieve one-step efficient enrichment of crosslinked peptides by specific binding with anti-TMT agarose spheres and improve water solubility and biocompatibility.
High coverage identification of in-situ crosslinking information of cell surface proteins is achieved, the identification efficiency of low-abundance proteins is improved, and crosslinking capture with high biocompatible and membrane-impermeable ability is achieved on the surface of living cells.