Tropical plant composition with soothing and repairing effects as well as preparation method and application of tropical plant composition

Abstract

The invention discloses a tropical plant composition with soothing and repairing functions as well as a preparation method and application of the tropical plant composition. The tropical plant composition mainly comprises the following components in parts by weight: 1-50 parts of a pandanus asiatica leaf extract, 1-50 parts of a desert rose extract, 1-30 parts of a plumeria rubra extract, 1-30 parts of an impatiens balsamina extract and 1-30 parts of a verbena officinalis extract. The invention further discloses a preparation method and application of the composition, the preparation method of the composition comprises the steps of low-temperature extraction, concentration and enrichment, feed liquid clarification, column chromatography adsorption, low-temperature drying and the like, active ingredients such as specific plant polysaccharides and total phenols can be effectively enriched, and the effects of inhibiting release of inflammatory factors, removing active oxygen and relieving and stabilizing skin are achieved. When being added into a daily chemical product as an active matter, the composition can play a good role in relieving and repairing, stabilizes and balances the skin from multiple dimensions, and has a good effect.

Description

Technical Field

[0001] The invention discloses a tropical plant composition with soothing and repairing effects, a preparation method and application thereof, and belongs to the technical field of cosmetic raw material preparation. Background Art

[0002] With the introduction of new cosmetics regulations and the gradual standardization of efficacy claims in the industry, "efficacy" has gradually become a key point that products pay more and more attention to. Many active ingredients are favored, but although some active ingredients have good efficacy, excessive addition or too many product efficacy ingredients can easily cause irritation in the product formula, resulting in symptoms such as redness, swelling, irritation, and discomfort. Therefore, soothing ingredients specifically for formulas and groups have gradually attracted people's attention. Developing raw materials with good soothing and repairing properties based on safety and stability has become the focus of many companies and even the industry.

[0003] Tropical plants refer to plants that live in tropical and subtropical regions. The tropics have the characteristics of small temperature changes and summer all year round, with an annual average temperature of 22-26°C. Due to the hot climate and abundant rainfall, tropical plants often have a wealth of plant active ingredients, and due to long-term growth under high ultraviolet radiation and high heat conditions, some tropical plants also produce more repair and stabilization ingredients to cope with nature. Based on this, this project attempts to develop soothing and repairing ingredients based on tropical plant active ingredients to add to daily chemical products to exert soothing and repairing effects.

[0004] CN117379354A discloses a repairing and soothing water containing rose extract and a preparation method thereof, which comprises rose hydrosol, moisturizer, antioxidant, skin conditioner and other ingredients. Although it has good soothing, anti-allergic and repairing effects, it is a single-formula product, not a raw material product, and has a relatively complex composition, which has great limitations in practical application.

[0005] CN109394615B discloses a soothing and repairing composition containing a variety of plant extracts. The soothing and repairing agent includes hydrolyzed lupine protein, long bean fruit extract and safflower flower extract. Although it has a soothing and repairing effect, the extraction process of the plant repairing ingredients is not described, which may easily cause differences in actual use.

[0006] Therefore, it is very necessary to develop a plant composition derived from tropical plants with soothing and repairing effects, and add it as an active ingredient to daily chemical products to achieve soothing and repairing effects such as soothing the skin, reducing skin irritation, and protecting the skin barrier. Summary of the invention

[0007] In view of the above-mentioned deficiencies, the purpose of the present invention is to provide a tropical plant composition with soothing and repairing effects. The composition has good soothing and repairing effects and can be applied to cosmetics to effectively remove excess free radicals, inhibit the release of skin inflammatory factors, and repair the skin barrier. The plant composition itself is safe, mild and non-irritating and can be widely used in the field of skin care.

[0008] To this end, the first technical solution provided by the present invention is as follows:

[0009] A tropical plant composition with soothing and repairing effects comprises the following ingredients in parts by weight: 1-50 parts of Pandanus odoratus leaf extract, 1-50 parts of Desert Rose extract, 1-30 parts of Plumeria extract and 1-30 parts of Impatiens balsamina extract.

[0010] Furthermore, the invention also comprises 1-30 parts of verbena extract.

[0011] Preferably, the composition is composed of the following ingredients in parts by weight: 1-50 parts of Pandanus odoratus leaf extract, 1-50 parts of Desert Rose extract, 1-30 parts of Plumeria dasyphylla extract, 1-30 parts of Impatiens balsamina extract and 1-30 parts of Verbena officinalis extract.

[0012] The plants in the present invention are all tropical plants, containing rich plant active ingredients, and the specific introduction is as follows:

[0013] Pandanus odoratus may be native to the Maluku Islands in Indonesia. It is mainly cultivated in Sri Lanka, Malaysia, Thailand and other countries. It is also cultivated in Hainan, my country. It likes to grow in tropical humid acidic soil. It is rich in plant polysaccharides, volatile oils, phenols and other ingredients, and can be used as a food flavoring.

[0014] Desert rose, native to Africa, prefers a hot, dry and sunny environment. It is rich in anthocyanins, polyphenols, volatile oils, etc., has excellent antioxidant effects, and has many values ​​such as medicinal and ornamental.

[0015] Plumeria, a plant of the Apocynaceae family, is native to the tropical regions of Mexico, Venezuela and the West Indies in America and is now widely planted in subtropical regions. It contains flavonoids and polysaccharides, and has the effect of clearing away heat and dampness.

[0016] Impatiens: A herbaceous plant of the genus Impatiens in the family Impatiens, native to India, Malaysia and China, it contains a variety of anthocyanins, flavonoids, etc., and has the effects of promoting blood circulation, relieving swelling, and antibacterial.

[0017] Verbena: A perennial herb of the Verbenaceae family, Verbena is widely distributed and mainly grown in tropical America, growing on roadsides at the foot of mountains or wastelands near villages. The whole plant is used as medicine, which has the effects of clearing away heat and detoxifying, promoting blood circulation and removing blood stasis, and promoting diuresis and reducing swelling.

[0018] In the plant extract composition of the present invention, the Pandanus odoratus leaf extract and the Desert Rose extract are the first active ingredients, which are mainly composed of plant polysaccharides, organic acids, and vitamins, and can form a film on the body surface to moisturize and repair the skin barrier; the plumeria, Impatiens balsamina, and Verbena are the second active ingredients, which are mainly composed of plant polyphenols and flavonoids, and can inhibit the release of inflammatory factors and remove excessive free radicals. The two are compounded, and at the same time, they are extracted and preserved at low temperature and freeze-dried to form, which can synergize and maximize the soothing and repairing effects, and have good application prospects in the cosmetics industry.

[0019] The second technical solution provided by the present invention is:

[0020] A method for preparing a tropical plant composition with soothing and repairing effects comprises the following steps:

[0021] S1, component A preparation: weigh dry Pandanus fragrant leaves, rose, crushed and mixed with a grinder, add extraction solvent, extract twice at low temperature, each time 1-3h, filter with 400 mesh filter cloth after extraction, filter out filter residue, collect and combine the two extracts, concentrate under reduced pressure to the weight equivalent to the weighed mass, and microfilter to obtain the filtrate to obtain component A;

[0022] S2, preparation of component B: weigh the dried frangipani, impatiens and verbena, crush and mix them evenly with a traditional Chinese medicine grinder, add an extraction solvent, extract twice at low temperature, each time for 1-3h, filter with a 400-mesh filter cloth after extraction, filter out the filter residue, collect and combine the two extracts; concentrate the obtained extract under reduced pressure to 3 times the mass of the original plant, microfiltrate to obtain the filtrate, then add the filtrate to a macroporous adsorption resin for column adsorption, the adsorption flow rate is 1BV / h, rinse with water for 3BV after adsorption, then elute with 3BV ethanol aqueous solution, collect the eluate, and then concentrate under reduced pressure to a mass equivalent to the weighed mass to obtain a concentrate, namely component B;

[0023] S3. Low-temperature drying: The above-mentioned component A and component B are mixed in equal amounts at a ratio of 1:1, stirred evenly, added with auxiliary materials, put into a freeze dryer for pre-freezing, and then sublimated and dried, and crushed to obtain the final plant composition.

[0024] Preferably, the extraction solvent in S1 is pure water, and the solid-liquid ratio is 1:5-1:30 (g / ml).

[0025] Preferably, the extraction solvent in S2 is 30-95% ethanol water (v / v), and the material-liquid ratio is 1:10-1:30 (g / ml).

[0026] Preferably, the low temperature in S1 and S2 ranges from 30°C to 60°C.

[0027] Preferably, the extraction method in S1 and S2 is any one of constant temperature stirring extraction, ultrasonic extraction and microwave extraction.

[0028] Preferably, the pore size of the microfiltration membrane in S1 and S2 is 0.20-0.45 μm.

[0029] Preferably, the macroporous adsorption resin in S2 is a non-polar or weakly polar macroporous resin with styrene divinylbenzene as the skeleton.

[0030] Preferably, the volume fraction of ethanol in the ethanol-water eluent in S2 is 40%-70%.

[0031] Preferably, the auxiliary material in S3 is starch or maltodextrin.

[0032] Preferably, the temperature of the freeze-drying pre-freezing stage in S3 is -30 to -70°C, and the freezing time is 4-8 hours; the air pressure of the sublimation stage is controlled to be 0-20Pa, and the time is 10-20 hours.

[0033] The third technical solution provided by the present invention is: application of the soothing and repairing plant composition, wherein the plant composition is applied in soothing and repairing cosmetics.

[0034] Preferably, the soothing and repairing cosmetic is a skin external preparation prepared with the composition having soothing and repairing effects as an active component and conventional pharmaceutical or cosmetic excipients or auxiliary ingredients.

[0035] Specifically, the soothing and repairing cosmetics are one or more of lotions, essences, creams, facial masks, and gels.

[0036] Compared with the prior art, the present invention has the following beneficial effects:

[0037] 1. The soothing and repairing plant composition disclosed in the present invention designs different extraction processes based on the characteristics of plants to extract specific plant polysaccharides, organic acids, specific plant polyphenols, flavonoids, etc., which can effectively remove excess free radicals, inhibit the release of skin inflammatory factors, and repair the skin barrier, and has excellent soothing and repairing effects.

[0038] 2. The plants selected in the present invention are all derived from specific tropical plants, which are rich in plant active ingredients. At the same time, they are scientifically matched to have a synergistic effect and better promote the efficacy.

[0039] 3. The temperature used in the whole process of the extraction and purification process of the present invention does not exceed 60°C, and the drying process is low-temperature freeze-drying, which retains the plant active ingredients to a greater extent and is not damaged by heat.

[0040] 4. The soothing and repairing plant composition disclosed in the present invention has the advantages of being pure natural, non-irritating, safe and non-toxic, can meet the needs of safe skin care, and has good promotion prospects and a broad application market. DETAILED DESCRIPTION

[0041] Many specific details are set forth in the following description to facilitate a full understanding of the present invention. However, the present invention can be implemented in many other ways than those described herein, and those skilled in the art can make similar modifications without violating the connotation of the present invention, so the present invention is not limited to the specific implementation disclosed below.

[0042] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present invention. The terms used in the specification of the present invention herein are only for the purpose of describing specific embodiments and are not intended to limit the present invention.

[0043] It should be understood that the specific embodiments described herein are only used to explain the present invention, and are not used to limit the present invention.

[0044] In the examples, unless otherwise specified, all the means used are conventional means in the art.

[0045] As used herein, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion.

[0046] For example, a composition, process, method, article, or apparatus that comprises a list of elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.

[0047] In addition, the technical features involved in the various embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0048] The reagents and instruments used in the examples and comparative examples of the present invention are all commercially available products.

[0049] The following is a further detailed description of the claims of the present invention in conjunction with specific implementation methods, but does not constitute any limitation to the present invention. Any limited modifications made by anyone within the scope of protection of the claims of the present invention are still within the scope of protection of the claims of the present invention.

[0050] Example 1

[0051] A tropical plant composition with soothing and repairing effects comprises the following components: 20g of pandanus leaves, 20g of roses, 20g of plumeria, 20g of impatiens, and 20g of verbena.

[0052] Example 2

[0053] A tropical plant composition with soothing and repairing effects comprises the following components: 50g of pandanus leaves, 1g of rose, 30g of plumeria, 10g of impatiens, and 9g of verbena.

[0054] Example 3

[0055] A tropical plant composition with soothing and repairing effects comprises the following components: 1g of pandanus leaves, 50g of roses, 30g of plumeria, 9g of impatiens, and 10g of verbena.

[0056] Example 4

[0057] A tropical plant composition with soothing and repairing effects comprises the following components: 25g of pandanus leaves, 25g of rose flowers, 1g of plumeria, 30g of impatiens, and 19g of verbena.

[0058] Example 5

[0059] A tropical plant composition with soothing and repairing effects comprises the following components: 10g of pandanus leaves, 30g of roses, 29g of plumeria, 1g of impatiens, and 30g of verbena.

[0060] Example 6

[0061] A tropical plant composition with soothing and repairing effects comprises the following components: 30g of pandanus leaves, 20g of roses, 20g of plumeria, 29g of impatiens and 1g of verbena.

[0062] Embodiment Preparation example:

[0063] S1, component A preparation: according to the above mass ratio, the dried Pandanus fragrant leaves and rose flowers were accurately weighed, crushed and mixed evenly with a traditional Chinese medicine grinder, and extracted with pure water at a solid-liquid ratio of 1:20 (g / ml), and the mixture was stirred and extracted at a constant temperature of 50°C for 2 times, each time for 2 hours. After the extraction, the mixture was filtered with a 400-mesh filter cloth to remove the filter residue, and the 2 extracts were collected and combined, and concentrated under reduced pressure to a mass equivalent to that of the plant, and the filtrate was obtained by microfiltration with a pore size of 0.45 μm to obtain component A;

[0064] S2, preparation of component B: accurately weigh dried frangipani, impatiens and verbena according to the above mass ratio, crush and mix evenly with a traditional Chinese medicine grinder, add 60% ethanol water (v / v) at a solid-liquid ratio of 1:30 (g / ml), ultrasonically extract twice at 60°C, each time for 1h, filter with a 400-mesh filter cloth after extraction, filter out the filter residue, collect and combine the two extracts; concentrate the obtained extract under reduced pressure to 3 times the mass of the original plant, microfilter with a pore size of 0.22μm to obtain the filtrate, then add the filtrate to a macroporous adsorption resin for column adsorption, the adsorption flow rate is 1BV / h, rinse with water for 3BV after adsorption, then elute with 3BV of 50% ethanol water (v / v) solution, collect the eluate, and then concentrate under reduced pressure to a mass equivalent to the plant to obtain a concentrate, namely component B;

[0065] S3. Low-temperature drying: The above-mentioned component A and component B are mixed in equal amounts at a ratio of 1:1, stirred evenly, added with malt starch, and put into a freeze dryer for pre-freezing at a temperature of -50°C for 6 hours, and then sublimated and dried in an environment with an air pressure of 2Pa for 15 hours. After drying, the mixture is crushed to obtain the final plant composition.

[0066] In order to demonstrate the effect of the present invention, the following comparative experimental examples are given:

[0067] The preparation method of any group of compositions in Comparative Examples 1-5 is the same as that in the examples.

[0068] Comparative Example 1

[0069] A tropical plant composition with soothing and repairing effects comprises the following components: 40g of pandanus leaves, 20g of rose flowers, 20g of plumeria flowers, and 20g of impatiens flowers.

[0070] Comparative Example 2

[0071] A tropical plant composition with soothing and repairing effects comprises the following components: 30g of rose, 30g of plumeria, 30g of impatiens and 10g of verbena.

[0072] Comparative Example 3

[0073] A tropical plant composition with soothing and repairing effects comprises the following components: 20g of pandanus leaves, 40g of rose flowers, 30g of impatiens and 10g of verbena.

[0074] Comparative Example 4

[0075] A tropical plant composition with soothing and repairing effects comprises the following components: 10g of pandanus leaves, 30g of rose flowers, 30g of plumeria and 30g of verbena.

[0076] Comparative Example 5

[0077] A tropical plant composition with soothing and repairing effects comprises the following components: 30g of pandanus odoratus leaves, 20g of plumeria, 30g of impatiens, and 20g of verbena.

[0078] The above-mentioned soothing and repairing plant composition can be used to prepare soothing and repairing cosmetics. The soothing and repairing cosmetics are skin external preparations prepared by using the soothing and repairing composition as an active component and adding conventional pharmaceutical or cosmetic excipients or auxiliary ingredients. When used specifically, the composition prepared by the present invention is directly added to the cosmetic matrix, and the addition amount can be adjusted according to actual needs, preferably 0.1wt% to 5wt%.

[0079] In order to better illustrate the advantages of the present invention, the efficacy experiment of the composition with soothing and repairing efficacy provided by the present invention is given below:

[0080] Test 1: Antioxidant test - ABTS free radical scavenging test

[0081] The soothing and repairing compositions prepared in Experimental Examples 1-6 and Comparative Examples 1-5 were tested. The tests are as follows:

[0082] 1. Preparation of ABTS+ standard stock solution:

[0083] Accurately weigh 0.0384 g ABTS+, dissolve it in distilled water and dilute it to a 10 mL volumetric flask to make the ABTS+ concentration 7 mmol·L -1 ; Accurately weigh 0.0066g K2S2O8 (potassium sulfate), dissolve it in distilled water and dilute it to a 10mL volumetric flask to make the concentration of K2S2O8 2.45mmol·L -1 Then, the two solutions were mixed in a brown bottle (1:1 mixture) and placed in a dark place at room temperature overnight (12-16 h) to obtain an ABTS+ solution.

[0084] The ABTS+ solution was diluted with phosphate buffer (10 mmol·L -1 , pH 7.4) (dilution factor of about 25 times) so that its absorbance at 30°C and 734nm wavelength is 0.700±0.020, thereby obtaining ABTS+ working solution.

[0085] 2. Sample clearance test

[0086] Take 1 mL of sample solution diluted to the same multiple into a test tube, add 3 mL of ABTS+ working solution, mix well, store at room temperature and away from light for 10 min, measure the absorbance at 734 nm, and use anhydrous ethanol as a blank control. Set vitamin C as a control, and calculate the scavenging rate of each extract on ABTS free radicals according to the following formula. The scavenging rate of ABTS+ is calculated as follows:

[0087] ABTS free radical scavenging rate (%) = [1-(Ai-Aj) / Ac] × 100%

[0088] Where: Ai is 1mL sample solution + 3mL ABTS + The absorbance value of the mixed solution of the solution; Aj is the absorbance value of the mixed solution of 1mL sample solution + 3mL anhydrous ethanol; Ac is the absorbance value of 1mL anhydrous ethanol + 3mL ABTS + The absorbance value of the solution mixture.

[0089] Table 1. Experimental results of the ability of the composition to scavenge ABTS free radicals

[0090] Sample name ABTS free radical scavenging rate Vitamin C 93.21％ Example 1 87.63％ Example 2 82.26％ Example 3 91.05％ Example 4 80.17％ Example 5 88.63％ Example 6 81.63％ Comparative Example 1 62.82％ Comparative Example 2 72.54％ Comparative Example 3 76.33％ Comparative Example 4 68.29％ Comparative Example 5 57.10％

[0091] As shown in Table 1, the scavenging rate of the reference vitamin C on ABTS free radicals is 93.21%, while the inhibition rate of Example 3 is 91.05%. The inhibition rates of the two are relatively close, and the scavenging effects of other examples are also above 80%, indicating that the soothing and repairing composition has a good scavenging effect on ABTS free radicals. At the same time, as shown in the above table, the scavenging rates of Examples 1-6 are all higher than those of Comparative Examples 1-5, indicating that the antioxidant capacity of the composition containing five plants is better than that of the composition lacking any plant extract, and the five plants have a synergistic effect, and the formula is reasonable, indicating that the composition has good antioxidant capacity, can protect the skin in a certain way, and is conducive to the soothing effect.

[0092] Experiment 2: Macrophage Inflammation Model Experiment

[0093] The soothing and repairing compositions prepared in Examples 1-6 and Comparative Examples 1-5 were tested as follows:

[0094] 1. Cytotoxicity test: Adjust the concentration of RAW264.7 cell suspension and add it to a 96-well cell plate. Culture it overnight at 37°C and 5% CO2 for detection. Dilute the test samples with cell culture medium to different concentrations and add them to the cell plate. Culture them at 37°C and 5% CO2 for 20 to 48 hours. Discard the culture medium, wash with PBS 1-2 times, add MTT working solution and continue to culture. After the culture is completed, discard the culture medium, add DMSO, shake and mix, and measure the absorbance at 570nm (with 630nm as the background). Calculate the cytotoxicity based on the results, and screen the sample concentration without obvious cytotoxicity for the next experiment based on the cell morphology.

[0095] 2. Inflammatory factor IL-6 test: Adjust the concentration of RAW264.7 cell suspension and add it to a 96-well cell plate. Culture it overnight at 37°C and 5% CO2 for detection. The test sample was diluted to a non-cytotoxic concentration with a cell culture medium containing LPS and then added. The culture medium containing LPS was used as a negative control (NC), and the cell culture medium without LPS was used as a blank control (BC). The positive control diluted with the cell culture medium containing LPS was used as a positive control (PC, dexamethasone sodium phosphate). After adding the sample, the cells continued to be cultured for 20 to 24 hours. After the action ended, the supernatant was taken and tested according to the operating procedures of the IL-6 Elisa kit.

[0096] Result calculation and judgment:

[0097] The calculation formula of the down-regulation rate of inflammatory factor IL-6 is:

[0098] Downward adjustment rate = (1-T / NC) × 100%

[0099] in:

[0100] T——average value of inflammatory factor IL-6 in the experimental group

[0101] NC——average value of inflammatory factor IL-6 in negative control group

[0102] The data were subjected to statistical and significance analysis, and the P value was calculated. P < 0.05 indicated a significant difference, otherwise there was no statistical difference.

[0103] When the IL-6 content of the negative control group is much higher than that of the blank control group, and there is a significant difference between the two (P < 0.05), it means that the experiment is established.

[0104] When the content of inflammatory factor IL-6 in the sample group is lower than that in the negative control group, and there is a significant difference between the two (P < 0.05), it can be determined that the sample has a soothing effect.

[0105] Table 2. Results of experimental study on the inhibition of inflammatory factors by the combination

[0106]

[0107]

[0108] Note: If P>0.05, it means there is no significant difference, recorded as "ns"; if P<0.05, it means there is a significant difference, and 0.01<P≤0.05, recorded as "*"; 0.001<P≤0.01, recorded as "**"; 0.0001<P≤0.001, recorded as "***"; P≤0.0001, recorded as "****"

[0109] The experimental results of Examples 1-6 and Comparative Examples 1-5 on the inhibition of the inflammatory factor IL-6 content are shown in Table 2 above, BC is the experimental blank control, NC is the experimental negative control, and the PC group is the positive control. Among them, the IL-6 content in the NC group is much higher than that in the blank control group, and there is a significant difference between the two (P < 0.05), indicating that the experiment is established. It can be seen from the table that when the same safe concentration of the embodiment and the comparative sample is added, Example 2 has the best effect, and the down-regulation rate of the IL-6 inflammatory factor caused by exogenous stimulation is 52.30%, which is significantly different from the negative group (P < 0.05), close to the positive control PC down-regulation rate. And the down-regulation rate of the cellular inflammatory factors in the embodiments is more than 30%, which is better than any comparative example, which once again shows the rationality of this combination and can synergize. At the same time, it shows that the soothing and repairing composition can effectively inhibit the release of the inflammatory factor IL-6, and has a good soothing effect in this pathway.

[0110] Experiment 3: Zebrafish embryo tail fin repair promotion test

[0111] 1. Experimental Principle

[0112] After the skin barrier is damaged, the body needs to speed up the growth of epidermal cells to repair the damaged skin barrier. The process and principle of zebrafish and human skin barrier damage repair are very consistent, and can be used as a detection and screening model for human skin repair efficacy. The experiment cut off the zebrafish tail fin to create a tail fin damage model, and evaluated the results of the sample's repair of the tail fin after administration, providing data support for the sample's function in promoting skin repair.

[0113] 2. Experimental Methods

[0114] Step 1: Select healthy zebrafish embryos that are 3 days old after fertilization;

[0115] Step 2: Resection of the caudal fin of zebrafish embryos to establish an injury model;

[0116] Step 3: Set different groups to culture for 48 hours after adding samples;

[0117] Step 4: Take photos under a fluorescence microscope or stereoscope;

[0118] Step 5: Data processing and analysis.

[0119] 3. Results and judgment

[0120] Tail fin repair promotion rate: Promotion rate = (T–C) / C×100%

[0121] Where: T is the average tail fin length of fish embryos in the test substance treatment group; C is the average tail fin length of fish embryos in the model control group.

[0122] A two-tailed T test was performed on the tail fin repair level of the fish embryos in the test substance treatment group and the model control group to obtain the p value. P < 0.05 indicates a significant difference, otherwise there is no statistical difference.

[0123] The test is valid if at least 90% of the fish embryos in each test group survive after exposure, and the promotion rate of tail fin repair of fish embryos in the positive control group is positive and P<0.05.

[0124] When the promotion rate of the test substance treatment group is positive and has a statistically significant difference compared with the model control group (P < 0.05), it can be considered that the sample has the effect of promoting tail fin repair.

[0125] After diluting the Examples 1-6 and Comparative Examples 1-5 to the same safe concentration, a zebrafish tail fin repair test was conducted. The results are shown in Table 3.

[0126] Table 3. Zebrafish embryo tail fin repair promotion test results

[0127] Group Mean caudal fin length Repair promotion rate P-value Significance Model control group 75.28±15.34 / / / Positive control group 102.54±14.66 36.21％ ＜0.0001 **** Example 1 97.80±20.32 29.91％ ＜0.0001 **** Example 2 92.64±14.08 23.06％ 0.0003 *** Example 3 95.33±12.34 26.63％ ＜0.0001 **** Example 4 88.26±20.58 17.24％ ＜0.0001 **** Example 5 85.37±17.20 18.40％ ＜0.0001 **** Example 6 90.54±21.06 20.27％ 0.0003 *** Comparative Example 1 82.24±11.03 9.25％ 0.0027 ** Comparative Example 2 79.45±17.65 5.54％ ＜0.0001 **** Comparative Example 3 74.32±24.65 -1.28％ 0.0526 ns Comparative Example 4 79.56±10.23 5.69％ 0.008 ** Comparative Example 5 75.65±12.34 0.49％ ＜0.0001 ****

[0128] Note: If P>0.05, it means there is no significant difference, which is recorded as “ns”; if P<0.05, it means there is a significant difference, and 0.01<P≤0.05, it is recorded as “*”; 0.001<P≤0.01, it is recorded as “**”; 0.0001<P≤0.001, it is recorded as “***”; P≤0.0001, it is recorded as “****”.

[0130] In this zebrafish embryo tail fin repair test, more than 90% of the fish embryos survived after the exposure of the test group, and the positive control group fish embryo tail fin repair promotion rate was a positive number of 36.21%, and P <0.05, indicating that the test was effective. As shown in Table 3, among the samples of Examples 1-6 and Comparative Examples 1-5, Example 1 had the best repair effect on the zebrafish embryo tail fin, with a repair promotion rate of 29.91%, which was slightly lower than the repair promotion rate of 36.21% in the positive control group, indicating that its repair effect was good. At the same time. From the analysis of the data, it can be seen that the samples of Examples 1-6 have good effects on the promotion rate of zebrafish embryo tail fin repair, all of which are above 17%, and better than any comparative sample, which once again shows the rationality of this formula compatibility, and the five plant compatibility can synergize. At the same time, it is shown that this soothing repair composition can effectively promote the repair of the fish embryo tail fin, and has a good potential repair effect when applied to cosmetics.

[0131] In summary, the present invention uses tropical plants as sources, selects Pandanus odoratus, Desert Rose, Plumeria, Impatiens and Verbena for scientific combination, and uses low-temperature extraction, concentration and enrichment, liquid clarification, column chromatography adsorption, low-temperature drying and other extraction and purification processes to directionally enrich the plant active ingredients to prepare a soothing and repairing composition, which can play a good role in inhibiting the release of inflammatory factors, scavenging active oxygen, soothing and stabilizing the skin. When added to daily chemical products, it has good soothing and repairing effects.

[0132] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. A tropical plant composition with soothing and repairing effects, characterized in that: The invention comprises the following ingredients in parts by weight: 1-50 parts of Pandanus odoratus leaf extract, 1-50 parts of Desert Rose extract, 1-30 parts of Plumeria dasyphylla extract and 1-30 parts of Impatiens balsamina extract.

2. The composition according to claim 1, characterized in that The invention also comprises 1-30 parts of verbena extract.

3. The composition according to claim 1, characterized in that The invention is composed of the following ingredients in parts by weight: 1-50 parts of Pandanus odoratus leaf extract, 1-50 parts of Desert Rose extract, 1-30 parts of Plumeria dasyphylla extract, 1-30 parts of Impatiens balsamina extract and 1-30 parts of Verbena officinalis extract.

4. A method for preparing the composition according to any one of claims 1 to 3, characterized in that: The following steps are involved: S1, component A preparation: weigh dry Pandanus fragrant leaves, rose, crushed and mixed with a grinder, add extraction solvent, extract twice at low temperature, each time 1-3h, filter with 400 mesh filter cloth after extraction, filter out filter residue, collect and combine the two extracts, concentrate under reduced pressure to the weight equivalent to the weighed mass, and microfilter to obtain the filtrate to obtain component A; S2, preparation of component B: weigh the dried frangipani, impatiens and verbena, crush and mix them evenly with a traditional Chinese medicine grinder, add an extraction solvent, extract twice at low temperature, each time for 1-3h, filter with a 400-mesh filter cloth after extraction, filter out the filter residue, collect and combine the two extracts; concentrate the obtained extract under reduced pressure to 3 times the mass of the original plant, microfiltrate to obtain the filtrate, then add the filtrate to a macroporous adsorption resin for column adsorption, the adsorption flow rate is 1BV / h, rinse with water for 3BV after adsorption, then elute with 3BV ethanol aqueous solution, collect the eluate, and then concentrate under reduced pressure to a mass equivalent to the weighed mass to obtain a concentrate, namely component B; S3. Low-temperature drying: The above-mentioned component A and component B are mixed in equal amounts at a ratio of 1:1, stirred evenly, added with auxiliary materials, put into a freeze dryer for pre-freezing, and then sublimated and dried, and crushed to obtain the final plant composition.

5. The preparation method according to claim 4, characterized in that: The extraction solvent in S1 is pure water, and the solid-liquid ratio is 1:5-1:30 (g / ml).

6. The preparation method according to claim 4, characterized in that: The extraction solvent in S2 is 30-95% ethanol water (v / v), and the material-liquid ratio is 1:10-1:30 (g / ml).

7. The preparation method according to claim 4, characterized in that: The low temperature range in S1 and S2 is 30° C.-60° C., the extraction method in S1 and S2 is any one of constant temperature stirring extraction, ultrasonic extraction and microwave extraction, and the pore size of the microfiltration membrane in S1 and S2 is 0.20-0.45 μm.

8. The preparation method according to claim 4, characterized in that: The macroporous adsorption resin in S2 is a non-polar or weakly polar macroporous resin with styrene divinylbenzene as a skeleton, and the volume fraction of ethanol in the eluent ethanol aqueous solution in S2 is 40%-70%.

9. The extraction method according to claim 4, characterized in that The auxiliary material in S3 is starch or maltodextrin. The temperature in the freeze-drying pre-freezing stage in S3 is -30 to -70°C, the freezing time is 4-8h, and the air pressure in the sublimation stage is controlled to be 0-20Pa for 10-20h.

10. Use of the composition according to any one of claims 1 to 3, characterized in that: The composition is used in the preparation of cosmetics, which are external skin preparations prepared by using the effective ingredients as active components and adding conventional pharmaceutical or cosmetic auxiliary materials or auxiliary ingredients.

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