Slow-release composition for solving ruminant rumen acidosis as well as preparation method and application thereof

Abstract

The invention relates to the technical field of feed additives, in particular to a sustained-release composition for solving ruminant rumen acidosis as well as a preparation method and application of the sustained-release composition. The composition is prepared from the following components in parts by weight: 30 to 60 parts of complex microbial inoculant capsules, 10 to 30 parts of thiamine, 5 to 15 parts of light calcium carbonate, 5 to 15 parts of magnesium oxide, 8 to 18 parts of sodium bicarbonate, 2 to 5 parts of rare earth elements, 3 to 8 parts of rhizoma coptidis, 5 to 15 parts of rhizoma atractylodis macrocephalae, 4 to 9 parts of poria cocos and 2 to 5 parts of rhizoma corydalis. The complex microbial inoculant capsule at least comprises parabacteroides dieldrii, bacillus subtilis, bacillus licheniformis, enterococcus faecalis and lactobacillus rosenbergii; the rare earth element is a mixture of lanthanum nitrate and cerous nitrate, and the mass ratio of lanthanum nitrate to cerous nitrate is 1: 1. The composition can be used for preventing and treating ruminant rumen acidosis, can be slowly released in the stomach due to the slow release effect, and does not need to be used as a feed additive all the time.

Description

Technical Field

[0001] The invention belongs to the technical field of feed additives, and in particular relates to a slow-release composition for solving rumen acidosis in ruminants, and a preparation method and application thereof. Background Art

[0002] Ruminal acidosis in ruminants is a metabolic acidosis caused by ruminants (such as cattle and sheep) who eat a large amount of carbohydrate-rich feed, produce excessive lactic acid in the rumen, and cause a sharp drop in the pH value in the rumen. This is mainly due to the sudden and large-scale consumption of grains (such as corn, wheat, etc.). These grains ferment rapidly in the rumen and produce a large amount of lactic acid. For example, in a dairy farm, if the proportion of concentrated feed suddenly increases, or the herd accidentally enters a warehouse where a large amount of grain is stored, it is easy to cause rumen acidosis. In addition, improper preparation of silage, excessive sugar content or excessive lactic acid fermentation may also cause rumen acidosis. In addition, poor feeding management can also cause this disease. For example, the breeder improperly controls the feed intake of ruminants, causing the animals to overeat; or the feeding method of the feed is unreasonable, and the principle of gradual transition is not followed, so that the rumen microorganisms cannot adapt to the changes in the feed.

[0003] In the prior art, sodium bicarbonate solution is generally used for intravenous injection to neutralize acidic substances in the blood. Of course, there are also some special targeted drugs, such as CN111346182B discloses a drug for treating rumen acidosis in ruminants and its preparation method, including corn sprout flour. This application has a better effect on the treatment of rumen acidosis in ruminants through the synergistic effect of corn sprout flour and sunflower oil, with quick effect and high cure rate. The two jointly promote the activity of the flora in the gastrointestinal tract of animals, balance the microecological environment, and regulate gastrointestinal function. However, it is impossible to prevent it in advance, and it can only be treated after cattle and sheep are sick.

[0004] For example, CN116211961A discloses a composition for preventing and treating ruminant rumen acidosis, an oral preparation containing the composition, a preparation method and a method of use, wherein the composition comprises the following components: red date powder, sapodilla, arborvitae leaves, bletilla striata, magnolia bark, radish seeds, abelia, fructus aurantii, tangerine peel, clove, patchouli, white peony root, atractylodes macrocephala, liquorice, active dry yeast, yeast extract and yeast cell wall. The composition defined in the application interacts with each other among various components, and can regulate the acid-producing bacteria in the rumen of ruminants, promote saliva secretion and rumination, neutralize gastric acid, protect gastrointestinal mucosa, balance the rumen, improve immunity, and prevent and treat ruminant rumen acidosis. However, although the application can be used as a feed additive to prevent the disease, it is easily digested by gastric acid, resulting in loss of effect over a long period of time, and its materials are expensive and cannot be used as a feed additive all the time.

[0005] In view of this, the present invention is proposed. Summary of the invention

[0006] The purpose of the present invention is to provide a sustained-release composition for solving rumen acidosis in ruminants, and a preparation method and application thereof. The composition obtained by the present invention can be used to prevent and treat rumen acidosis in ruminants, and due to its sustained-release effect, it can be slowly released in the stomach and does not need to be used as a feed additive all the time.

[0007] In order to achieve the above object, the present invention provides the following technical solutions:

[0008] In a first aspect, an embodiment of the present invention provides a sustained-release composition for solving rumen acidosis in ruminants, comprising the following components by weight: 30-60 parts of composite bacterial capsules, 10-30 parts of thiamine, 5-15 parts of light calcium carbonate, 5-15 parts of magnesium oxide, 8-18 parts of sodium bicarbonate, 2-5 parts of rare earth elements, 3-8 parts of coptis root, 5-15 parts of atractylodes, 4-9 parts of tuckahoe, and 2-5 parts of corydalis;

[0009] The composite bacterial capsule at least includes Parabacteroides distiliensis, Bacillus subtilis, Bacillus licheniformis, Enterococcus faecalis and Lactobacillus reuteri;

[0010] The rare earth element is a mixture of lanthanum nitrate and cerium nitrate, and the mass ratio of the lanthanum nitrate to the cerium nitrate is 1:1.

[0011] The composition obtained by compounding the present invention can effectively alleviate rumen acidosis in ruminants, and can inhibit pathogenic bacteria, promote the growth of beneficial bacteria, regulate acid-producing bacteria, inhibit the production of lactic acid and toxins, protect gastrointestinal mucosa, promote saliva secretion, and enhance immunity and digestion ability through the synergistic effect of multiple components.

[0012] When ruminants suffer from rumen acidosis, a large amount of lactic acid is produced in their stomachs. At this time, the compound composition of the present invention first stabilizes the pH, wherein sodium bicarbonate can quickly neutralize the acidic substances in the rumen and quickly increase the rumen pH value. Light calcium carbonate can also effectively neutralize gastric acid, and its effect is relatively mild and lasting. Magnesium oxide also has the ability to neutralize acidic substances, and its effect is relatively slow but lasting. The three work together to play a neutralizing role at different stages and degrees, and more effectively stabilize the rumen environment. The use of sodium bicarbonate alone may cause the rumen pH value to rise rapidly, fluctuate greatly, and cause great gastrointestinal stress. The addition of magnesium oxide and light calcium carbonate can buffer this rapid change, make the increase of rumen pH value more stable, and reduce the adverse effects of large fluctuations in pH value on rumen microorganisms and animal bodies. In addition, magnesium ions in magnesium oxide, sodium ions in sodium bicarbonate, and calcium ions in light calcium carbonate can jointly participate in maintaining the balance of osmotic pressure inside and outside ruminant cells, ensure the normal operation of physiological functions in the animal body, enhance the animal's resistance to rumen acidosis and other stress factors, and help animals maintain a good physiological state under rumen acidosis.

[0013] Then, the thiamine added by the present invention participates in the cellular ketoacid metabolism as a coenzyme, which can promote the growth of rumen lactic acid-utilizing bacteria and inhibit the reproduction of lactic acid-producing bacteria, thereby regulating the balance of lactic acid-producing bacteria and lactic acid-utilizing bacteria in the rumen and reducing the accumulation of lactic acid in the rumen. That is, by promoting the growth of lactic acid-utilizing bacteria and regulating carbohydrate metabolism, the rumen pH value is indirectly stabilized to prevent the pH value from dropping sharply due to the large-scale production of acidic substances such as lactic acid.

[0014] At the same time, the coptis root, atractylodes macrocephala, tuckahoe, and corydalis in the composite composition of the present invention can coordinately solve the problem of excessive rumen lactic acid by regulating the balance of rumen flora, promoting saliva secretion, protecting gastrointestinal mucosa, and regulating the body's acid-base balance. Among them, the bitter components of coptis root can stimulate the taste receptors of ruminants and reflexively increase saliva secretion. Saliva contains buffer substances such as sodium bicarbonate, which can neutralize the acidic substances produced in the rumen, maintain the relative stability of the rumen pH value, and alleviate the symptoms of rumen acidosis. In addition, components such as berberine can regulate the body's metabolic process, affect the production and excretion of acid-base substances, thereby producing a certain regulatory effect on the body's acid-base balance, which helps to alleviate the body's acidosis state caused by rumen acidosis. The volatile oil and other ingredients in Atractylodes macrocephala have a certain regulatory effect on rumen microorganisms, which can promote the growth and reproduction of beneficial microorganisms, help maintain the stability of rumen microecology, keep the microbial community in the rumen relatively balanced, and help prevent the accumulation of lactic acid caused by dysbacteriosis. At the same time, the polysaccharide components of Poria cocos can regulate the immune function of the animal body, thereby indirectly affecting the microbial environment in the rumen, enhancing the body's resistance to harmful bacteria, promoting the colonization and growth of beneficial bacteria, and making the acid-producing and acid-utilizing microorganisms in the rumen reach a relative balance, maintaining the normal physiological function of the rumen. Finally, Corydalis yanhusuo has the effect of promoting blood circulation and qi, which can improve the blood circulation of gastrointestinal mucosa, increase the nutrient supply of mucosa, promote the repair and regeneration of mucosa, and relieve the spasm of gastrointestinal smooth muscle, reduce the symptoms of gastrointestinal discomfort caused by rumen acidosis, and help protect the normal structure and function of gastrointestinal mucosa. In this stage, the synergistic effect of thiamine and various Chinese medicine ingredients allows the excessive lactic acid to be quickly resolved.

[0015] Finally, the present invention uses a self-made composite bacterial capsule to slowly release Parabacteroides distilogenes, Bacillus subtilis, Bacillus licheniformis, Enterococcus faecalis and Lactobacillus reuteri in the stomach, which can slowly repair the poisoning effect and can continue to take effect in the body, and does not need to be used as a feed additive all the time.

[0016] Among them, Parabacteroides distilogenes can produce volatile fatty acids such as acetic acid and propionic acid, as well as a large amount of succinic acid, which increases the ratio of propionic acid to butyric acid in the rumen of ruminants, thereby improving the rumen fermentation pattern, reducing the production of acidic substances such as lactic acid, and maintaining the stability of the rumen environment; Bacillus subtilis can regulate the microbial community structure in the rumen, inhibit the growth of harmful bacteria such as Escherichia coli, Salmonella, and Clostridium perfringens, and promote the proliferation of anaerobic probiotics such as Bifidobacterium and Lactobacillus, so that the rumen microbial flora tends to be stable and reduce the proliferation of harmful bacteria. The possibility of rumen acidosis caused by acidic substances produced by reproduction; Bacillus licheniformis can produce antimicrobial active substances, kill or inhibit pathogenic bacteria such as Staphylococcus aureus and yeast in the rumen, and at the same time promote the growth of beneficial bacteria such as Peptostreptococcus, Bifidobacterium, Lactobacillus, Bacteroides, etc., regulate the balance of flora in the rumen, maintain normal digestion and metabolic functions of the rumen, and reduce the incidence of rumen acidosis; Enterococcus faecalis can produce lactic acid, bacteriocins and other substances, on the one hand, which can inhibit the growth of pathogenic bacteria in the rumen, and on the other hand, regulate the rumen pH value through its own metabolic activities to maintain it within a relatively stable range, preventing rumen acidosis caused by too low pH value; and Lactobacillus reuteri can provide growth factors for lactic acid-utilizing bacteria in the rumen, promote their growth and reproduction, and enable lactic acid to be timely and effectively utilized and converted, thereby reducing the concentration of lactic acid in the rumen and alleviating the symptoms of rumen acidosis.

[0017] In addition, Bacillus subtilis can secrete a variety of digestive enzymes, such as cellulase and amylase, to help ruminants better digest and absorb nutrients in feed. Parabacteroides dieldrinii also participates in the metabolism and conversion of nutrients. The two work synergistically to improve the digestibility and utilization of feed, reduce the production of acidic substances by fermentation of undigested feed in the rumen, and thus prevent rumen acidosis. For example, the small molecules produced by the decomposition of cellulose by Bacillus subtilis can be further utilized and converted by Parabacteroides dieldrinii, providing more nutrients for ruminants while reducing the fermentation pressure in the rumen. At this time, Bacillus licheniformis secretes some enzyme substances to help decompose the nutrients in the feed, and work together with Bacillus subtilis to promote the digestion and absorption of nutrients, improve the production performance of ruminants, and enhance their ability to resist rumen acidosis. Enterococcus faecalis can promote the synthesis of a large number of vitamins, enzymes and other substances in the intestines, further improve the digestion and utilization rate of feed, provide more nutrients for ruminants, enhance their physical fitness and ability to resist rumen acidosis. Similarly, Lactobacillus reuteri can produce some enzyme substances that aid digestion, help decompose the nutrients in the feed, improve the digestibility and utilization rate of the feed, promote the absorption of nutrients, and reduce the amount of acidic substances produced by fermentation of undigested feed in the rumen, thereby preventing rumen acidosis. That is, the compounded strains of the present invention have a synergistic effect in nutrient absorption. In addition, the compounded strains of the present invention can also improve the body's immunity, enhance the disease resistance and stress resistance of ruminants, reduce rumen dysfunction caused by disease and stress, and thus prevent the occurrence of rumen acidosis. Moreover, the sustained-release effect of the composite bacterial agent capsule of the present invention can continuously improve the body's immunity of animals for a long time.

[0018] In addition, the lanthanum ions and cerium ions added in the present invention can promote the growth and reproduction of the bacteria in the composite bacterial capsule and the lactic acid-utilizing bacteria in the rumen. Specifically, the lanthanum ions and cerium ions can stabilize the active conformation of the enzymes inside the bacteria, thereby improving the activity of the enzymes, accelerating the metabolic reaction rate in the bacteria, and providing more energy and material basis for the growth of the bacteria. More importantly, lanthanum ions and cerium ions can regulate the permeability of bacterial cell membranes, making the material exchange inside and outside the cells smoother. On the one hand, appropriately increasing the permeability of the cell membrane to nutrients makes it easier for bacteria to absorb more nutrients, such as amino acids, sugars, etc., to provide sufficient material support for bacterial growth and reproduction; on the other hand, it also helps to discharge metabolic waste, maintain the stability of the intracellular environment, and promote the normal growth of bacteria. In a preferred embodiment, the composite bacterial capsule includes the following preparation steps:

[0019] Sodium alginate, glycerol, trehalose and gelatin are dissolved in water, and then Parabacteroides distiliensis, Bacillus subtilis, Bacillus licheniformis, Enterococcus faecalis and Lactobacillus roxburghii are added, mixed evenly, and calcium lactate is added. After reaction, the composite bacterial capsule is obtained by freeze-drying.

[0020] In a preferred embodiment, the mass ratio of sodium alginate, glycerol, trehalose, gelatin and calcium lactate is (0.5-5g): (4-9g): (4-9g): (2-4g): (5-10g).

[0021] In a preferred embodiment, the amount of the Parabacteroides distiliensis is 1×10 10 -2×10 11 CFU / g, the amount of Bacillus subtilis is 1×10 10 -2×10 11 CFU / g, the dosage of Bacillus licheniformis is 1×10 10 -2×10 11 CFU / g, the amount of Enterococcus faecalis is 1×10 10 -2×10 11 CFU / g, the dosage of Lactobacillus reuteri is 1×10 10 -2×10 11 CFU / g.

[0022] The present invention forms a protective function for the composite bacteria through a self-made capsule. Due to the complex and variable environment in the rumen, when rumen acidosis occurs, the rumen pH value will drop significantly, and some bacteria may find it difficult to adapt to this overly acidic environment, resulting in inhibition of their growth and reproduction, or even mass death, thereby failing to effectively exert their normal physiological functions to regulate the rumen environment and digestive function. At this time, the self-made capsule of the present invention protects the bacteria from the influence of the extreme environment, so that after controlling the pH of the composite composition of the present invention, the composite bacteria can exert their complete performance. In addition, in essence, when solving rumen acidosis, some of the introduced bacteria may break the original balance of the microbial flora in the rumen. The rumen microbial community is a relatively stable and complex ecosystem, and there is a relationship of mutual restriction and interdependence between various microorganisms. If the newly introduced bacteria over-breed, it may inhibit the growth of other beneficial bacteria, or compete with the original bacteria for nutrients and living space, but lead to an imbalance of the rumen microbial community, further affecting the normal function and health of the rumen. Therefore, the present invention encapsulates the bacteria through self-made capsules, so that the bacteria cannot directly compete with the microbial community in the rumen, but are slowly released and gradually increase so that the microbial community gradually reaches a balance.

[0023] In a second aspect, an embodiment of the present invention provides a method for preparing the above-mentioned sustained-release composition for solving rumen acidosis in ruminants, comprising the following steps:

[0024] Thiamine, light calcium carbonate, magnesium oxide and sodium bicarbonate are mixed evenly, and then the composite bacterial agent capsule is added and mixed evenly to obtain a composition.

[0025] In a third aspect, an embodiment of the present invention provides a feed additive, wherein the feed additive includes the above-mentioned sustained-release composition for solving rumen acidosis in ruminants.

[0026] In a fourth aspect, an embodiment of the present invention provides a feed, which includes the above-mentioned feed additive or the above-mentioned sustained-release composition for solving rumen acidosis in ruminants.

[0027] Compared with the prior art, the advantages and beneficial effects of the present invention are:

[0028] 1. The composition obtained by compounding of the present invention can effectively alleviate rumen acidosis in ruminants. Through the synergistic effect of multiple components, it can inhibit pathogenic bacteria, promote the growth of beneficial bacteria, regulate acid-producing bacteria, inhibit the production of lactic acid and toxins, protect gastrointestinal mucosa, promote saliva secretion, and enhance immunity and digestion ability.

[0029] 2. The compounded strains of the present invention have a synergistic effect in nutrient absorption. In addition, the compounded strains of the present invention can also improve the body's immunity, enhance the disease resistance and stress resistance of ruminants, reduce rumen dysfunction caused by disease and stress, and thus prevent the occurrence of rumen acidosis. Moreover, the sustained-release effect of the composite bacterial agent capsule of the present invention can continuously improve the body's immunity of animals for a long time.

[0030] 3. The present invention encapsulates the bacteria through self-made capsules, so that the bacteria cannot directly compete with the microbial community in the rumen, but are slowly released and gradually increase so that the microbial community gradually reaches a balance. DETAILED DESCRIPTION

[0031] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] Unless otherwise specified, the reagents mentioned in this example are commercially available.

[0033] Parabacteroides distilogenes, numbered CGMCC 1.5025, was purchased from Wuhan Huizao Biotechnology Co., Ltd.

[0034] Bacillus subtilis, CCTCC NO:M2022226, was purchased from Jiangmen Aobao Biotechnology Co., Ltd.;

[0035] Bacillus licheniformis, numbered CGMCC NO: 25162, was purchased from Shenyang Hongjiuzhou Chemical Co., Ltd.;

[0036] Enterococcus faecalis, numbered CGMCC NO.16954, was purchased from Green Farmer Biotechnology Co., Ltd.;

[0037] Lactobacillus reuteri, numbered CGMCC NO.25389, was purchased from Wuhan Huayuan Chentai Health Biotechnology Co., Ltd.;

[0038] Thiamine was purchased from Wuhan Belleye Biopharmaceutical Technology Co., Ltd.

[0039] Example 1

[0040] The present embodiment provides a sustained-release composition for solving rumen acidosis in ruminants, comprising the following components by weight: 50 parts of composite bacterial capsules, 15 parts of thiamine, 10 parts of light calcium carbonate with an average particle size of 100 μm, 10 parts of magnesium oxide, 15 parts of sodium bicarbonate, 2 parts of lanthanum nitrate, 2 parts of cerium nitrate, 4 parts of Coptis chinensis, 10 parts of Atractylodes macrocephala, 6 parts of Poria cocos, and 3 parts of Corydalis yanhusuo.

[0041] The preparation method of the composition is as follows: coptis root, atractylodes, tuckahoe and yanhusuo are mixed evenly according to a proportion, then lanthanum nitrate and cerium nitrate are added, ball milled at 200 r / min for 10 minutes, thiamine, light calcium carbonate, magnesium oxide and sodium bicarbonate are added, mixed and ball milled at 100 r / min for 5 minutes, and the obtained solid phase is taken out and mixed evenly with the composite bacterial agent capsule to obtain the composition.

[0042] The composite bacterial capsule comprises the following preparation steps:

[0043] 4 g of sodium alginate, 8 g of glycerol, 7 g of trehalose, and 3 g of gelatin were dissolved in water, and then Parabacteroides distiliensis, Bacillus subtilis, Bacillus licheniformis, Enterococcus faecalis, and Lactobacillus rosenbergii were added. The amount of Parabacteroides distiliensis was 1.6 × 10 10 CFU / g, the dosage of Bacillus subtilis is 1.6×10 10 CFU / g, the dosage of Bacillus licheniformis is 1.6×10 10 CFU / g, the dosage for Enterococcus faecalis is 1.6×10 10 CFU / g, the dosage of Lactobacillus reuteri is 1.6×10 10 CFU / g, mix evenly and add 8g calcium lactate, and freeze-dry after reaction to obtain the composite bacterial capsule.

[0044] Example 2

[0045] The present embodiment provides a sustained-release composition for solving rumen acidosis in ruminants, comprising the following components by weight: 60 parts of composite bacterial capsules, 12 parts of thiamine, 10 parts of light calcium carbonate with an average particle size of 100 μm, 5 parts of magnesium oxide, 13 parts of sodium bicarbonate, 2 parts of lanthanum nitrate, 2 parts of cerium nitrate, 6 parts of Coptis chinensis, 13 parts of Atractylodes macrocephala, 8 parts of Poria cocos, and 3 parts of Corydalis yanhusuo.

[0046] The preparation method of the composition is as follows: coptis root, atractylodes, tuckahoe and yanhusuo are mixed evenly according to a proportion, then lanthanum nitrate and cerium nitrate are added, ball milled at 200 r / min for 10 minutes, thiamine, light calcium carbonate, magnesium oxide and sodium bicarbonate are added, mixed and ball milled at 100 r / min for 5 minutes, and the obtained solid phase is taken out and mixed evenly with the composite bacterial agent capsule to obtain the composition.

[0047] The composite bacterial capsule comprises the following preparation steps:

[0048] 4 g sodium alginate, 8 g glycerol, 7 g trehalose, and 3 g gelatin were dissolved in water, and then Parabacteroides distiliensis, Bacillus subtilis, Bacillus licheniformis, Enterococcus faecalis, and Lactobacillus rosenbergii were added. The amount of Parabacteroides distiliensis was 1.6 × 10 10 CFU / g, the dosage of Bacillus subtilis is 1.6×10 10 CFU / g, the dosage of Bacillus licheniformis is 1.6×10 10 CFU / g, the dosage for Enterococcus faecalis is 1.6×10 10 CFU / g, the dosage of Lactobacillus reuteri is 1.6×10 10 CFU / g, mix evenly and add 8g calcium lactate, and freeze-dry after reaction to obtain the composite bacterial capsule.

[0049] Comparative Example 1

[0050] Compared with Example 1, the only difference is that: Parabacteroides distiliensis is not added, and the amount of Bacillus subtilis is 2×10 10 CFU / g, the dosage of Bacillus licheniformis is 2×10 10 CFU / g, the dosage for Enterococcus faecalis is 2×10 10 CFU / g, the dosage of Lactobacillus reuteri is 2×10 10 CFU / g.

[0051] Comparative Example 2

[0052] Compared with Example 1, the only difference is that Bacillus subtilis is not added, and the amount of Parabacteroides distiliensis is 2×10 10 CFU / g, the dosage of Bacillus licheniformis is 2×10 10 CFU / g, the dosage for Enterococcus faecalis is 2×10 10CFU / g, the dosage of Lactobacillus reuteri is 2×10 10 CFU / g.

[0053] Comparative Example 3

[0054] Compared with Example 1, the only difference is that Bacillus licheniformis is not added, and the amount of Parabacteroides distiliensis is 2×10 10 CFU / g, the dosage of Bacillus subtilis is 2×10 10 CFU / g, the dosage for Enterococcus faecalis is 2×10 10 CFU / g, the dosage of Lactobacillus reuteri is 2×10 10 CFU / g.

[0055] Comparative Example 4

[0056] Compared with Example 1, the only difference is that no Enterococcus faecalis was added, and the amount of Parabacteroides distiliensis was 2×10 10 CFU / g, the dosage of Bacillus subtilis is 2×10 10 CFU / g, the dosage of Bacillus licheniformis is 2×10 10 CFU / g, the dosage of Lactobacillus reuteri is 2×10 10 CFU / g.

[0057] Comparative Example 5

[0058] Compared with Example 1, the only difference is that Lactobacillus reuteri is not added, and the amount of Parabacteroides distiliensis is 2×10 10 CFU / g, the dosage of Bacillus subtilis is 2×10 10 CFU / g, the dosage of Bacillus licheniformis is 2×10 10 CFU / g, the dosage for Enterococcus faecalis is 2×10 10 CFU / g.

[0059] Comparative Example 6

[0060] Compared with Example 1, the only difference is that: no composite bacterial agent capsule is added, and the composite bacteria are directly added. Specifically, the dosage of Parabacteroides distilogenes is 1.6×10 10 CFU / g, the dosage of Bacillus subtilis is 1.6×10 10 CFU / g, the dosage of Bacillus licheniformis is 1.6×10 10 CFU / g, the dosage for Enterococcus faecalis is 1.6×10 10 CFU / g, the dosage of Lactobacillus reuteri is 1.6×10 10 CFU / g.

[0061] Comparative Example 7

[0062] Compared with Example 1, the only difference is that no light calcium carbonate is added, 15 parts of magnesium oxide and 20 parts of sodium bicarbonate are added.

[0063] Comparative Example 8

[0064] Compared with Example 1, the only difference is: no magnesium oxide is added, 15 parts of light calcium carbonate and 20 parts of sodium bicarbonate are added.

[0065] Comparative Example 9

[0066] Compared with Example 1, the only difference is that no sodium bicarbonate is added, 17.5 parts of light calcium carbonate and 17.5 parts of magnesium oxide are added.

[0067] Comparative Example 10

[0068] Compared with Example 1, the only difference is that thiamine is not added.

[0069] Comparative Example 11

[0070] Compared with Example 1, the only difference is that no lanthanum nitrate is added and 4 parts of cerium nitrate are added.

[0071] Comparative Example 12

[0072] Compared with Example 1, the only difference is that no cerium nitrate is added and 4 parts of lanthanum nitrate are added.

[0073] Comparative Example 13

[0074] Compared with Example 1, the only difference is that no rare earth element is added.

[0075] Performance Testing

[0076] 1. 240 cashmere goats with rumen acidosis were selected, and the pH value of rumen acid solution extracted was between 5.0 and 5.3. The goats were randomly divided into 12 groups, and the compositions prepared in the embodiments and comparative examples were respectively administered orally at a dosage of 0.1 g / kg, once. Other feeding conditions were the same, and the rumen acid was tested to see whether it was normal. The normal value was between 6.2 and 6.8. The number of cashmere goats cured was calculated. The results are shown in Table 1.

[0077] 240 normal cashmere goats were selected and fed with conventional diets. They were randomly divided into 12 groups and were gavaged with the compositions prepared in the embodiments and comparative examples once a week for 2 consecutive months. The basic diet (free access to hay, concentrate formula: corn, Nomin 6530 concentrate, bran mixed at a ratio of 65:30:5) and feeding management were consistent. The effect of the rumen acidosis prevention test was observed, and the number of rumen acidosis cases within two months of feeding was calculated. The results are shown in Table 1.

[0078] 240 normal cashmere goats were selected and fed with conventional diets. They were randomly divided into 12 groups and were respectively gavaged with the compositions prepared in the embodiments and comparative examples once a week for 2 consecutive months. The basal diet (free access to hay, concentrate formula: corn, Nomin 6530 concentrate, and bran mixed at a ratio of 65:30:5) and feeding management were consistent. The effect of the rumen acidosis prevention test was observed, and the average daily weight gain and diarrhea rate of the cashmere goats were comprehensively calculated. The diarrhea rate = the number of diarrhea times of the cashmere goats in two months / the total number of cashmere goats * 60 * 100%. The results are shown in Table 1 below.

[0079] Table 1 Performance test results

[0080] Number of cured / Number of rumen acidosis / cases Average daily weight gain / g Diarrhea rate / % Example 1 19 0 321 2.15 Example 2 20 0 332 1.89 Comparative Example 1 15 3 258 3.68 Comparative Example 2 13 5 252 3.54 Comparative Example 3 16 2 241 3.85 Comparative Example 4 14 5 238 3.71 Comparative Example 5 15 4 156 6.21 Comparative Example 6 8 9 284 2.98 Comparative Example 7 16 3 292 2.95 Comparative Example 8 15 4 289 2.91 Comparative Example 9 13 6 299 2.84 Comparative Example 10 16 2 283 2.79 Comparative Example 11 13 7 269 3.41 Comparative Example 12 12 5 272 3.49 Comparative Example 13 9 10 253 3.69

[0081] 2. 240 cattle with rumen acidosis were selected, and the pH value of rumen acid liquid extracted was between 5.0 and 5.3. The cattle were randomly divided into 12 groups, and the compositions prepared in the embodiments and comparative examples were respectively administered orally at a dosage of 0.1 g / kg, once. Other feeding conditions were the same. The rumen acid was tested to see whether it was normal. The normal value was between 6.2 and 6.8. The number of cured cattle was calculated. The results are shown in Table 2.

[0082] 240 normal cashmere goats were selected and fed with conventional diets. They were randomly divided into 12 groups and were gavaged with the compositions prepared in the embodiments and comparative examples once a week for 2 consecutive months. The basic diet (free access to hay, concentrate formula: corn, Nomin 6530 concentrate, bran mixed at a ratio of 65:30:5) and feeding management were consistent. The effect of the rumen acidosis prevention test was observed, and the number of rumen acidosis cases within two months of feeding was calculated. The results are shown in Table 2.

[0083] 240 normal cattle were selected and fed with conventional diets. They were randomly divided into 12 groups and were gavaged with the compositions prepared in the embodiments and comparative examples once a week for 2 consecutive months. The basal diet (free access to hay, concentrate formula: corn, Nomin 6530 concentrate, and bran mixed at a ratio of 65:30:5) and feeding management were consistent. The effect of the rumen acidosis prevention test was observed, and the average daily weight gain and diarrhea rate of the cattle were comprehensively calculated. The diarrhea rate was calculated as the percentage of the ratio of the number of diarrhea times of the cattle in two months to the total number of cattle multiplied by 60. The results are shown in Table 2 below.

[0084] Table 2 Performance test results

[0085] Number of cured / Number of rumen acidosis / cases Average daily weight gain / g Diarrhea rate / % Example 1 20 0 534 1.85 Example 2 20 0 564 1.64 Comparative Example 1 16 3 438 3.45 Comparative Example 2 14 4 428 3.28 Comparative Example 3 15 3 406 3.75 Comparative Example 4 13 6 401 3.68 Comparative Example 5 14 5 265 6.96 Comparative Example 6 7 10 486 2.84 Comparative Example 7 15 4 495 2.89 Comparative Example 8 16 3 492 2.96 Comparative Example 9 12 7 508 2.74 Comparative Example 10 15 4 481 2.68 Comparative Example 11 14 8 452 3.63 Comparative Example 12 11 6 461 3.41 Comparative Example 13 8 10 430 3.72

[0086] It can be seen from the above performance test results that the compositions obtained in Examples 1-2 have excellent prevention and treatment of ruminant rumen acidosis, and due to its sustained release effect, it can be slowly released in the stomach, does not need to be used as a feed additive all the time, and also has a strong preventive effect and animal growth promotion effect, especially the comprehensive performance of Example 2 is the most outstanding, which is mainly due to the three light calcium carbonate, magnesium oxide and sodium bicarbonate for adjusting pH, thiamine for indirect pH stabilization, and composite bacteria for preventing and curing gastric acidosis. The comparative example is significantly worse than the example in the corresponding performance test because it does not adopt the necessary technical solution, which better proves the irreplaceable nature of the specific technical solution of the present application for achieving technical effects and solving technical problems.

[0087] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A sustained-release composition for treating rumen acidosis in ruminants, characterized in that: The invention comprises the following components by weight: 30-60 parts of compound bacterial capsules, 10-30 parts of thiamine, 5-15 parts of light calcium carbonate, 5-15 parts of magnesium oxide, 8-18 parts of sodium bicarbonate, 2-5 parts of rare earth elements, 3-8 parts of coptis root, 5-15 parts of atractylodes macrocephala, 4-9 parts of tuckahoe, and 2-5 parts of corydalis; The composite bacterial capsule at least includes Parabacteroides distiliensis, Bacillus subtilis, Bacillus licheniformis, Enterococcus faecalis and Lactobacillus reuteri; The rare earth element is a mixture of lanthanum nitrate and cerium nitrate, and the mass ratio of the lanthanum nitrate to the cerium nitrate is 1:

1.

2. The sustained-release composition for treating rumen acidosis in ruminants according to claim 1, characterized in that: The composite bacterial agent capsule comprises the following preparation steps: Sodium alginate, glycerol, trehalose and gelatin are dissolved in water, and then Parabacteroides distiliensis, Bacillus subtilis, Bacillus licheniformis, Enterococcus faecalis and Lactobacillus roxburghii are added, mixed evenly, and calcium lactate is added. After reaction, the composite bacterial capsule is obtained by freeze-drying.

3. The sustained-release composition for treating rumen acidosis in ruminants according to claim 2, characterized in that: The mass ratio of the sodium alginate, glycerol, trehalose, gelatin and calcium lactate is (0.5-5g): (4-9g): (4-9g): (2-4g): (5-10g).

4. The sustained-release composition for treating rumen acidosis in ruminants according to claim 2, characterized in that: Relative to the mass of the sodium alginate, the amount of the Parabacteroides distiliensis is 1×10 10 -2×10 11 CFU / g.

5. The sustained-release composition for treating rumen acidosis in ruminants according to claim 2, characterized in that: Relative to the mass of the sodium alginate, the amount of Bacillus subtilis is 1×10 10 -2×10 11 CFU / g.

6. The sustained-release composition for treating rumen acidosis in ruminants according to claim 2, characterized in that: Relative to the mass of the sodium alginate, the amount of the Bacillus licheniformis is 1×10 10 -2×10 11 CFU / g.

7. The sustained-release composition for treating rumen acidosis in ruminants according to claim 2, characterized in that: Relative to the mass of the sodium alginate, the amount of Enterococcus faecalis is 1×10 10 -2×10 11 CFU / g, the dosage of Lactobacillus reuteri is 1×10 10 -2×10 11 CFU / g.

8. A method for preparing a sustained-release composition for treating rumen acidosis in ruminants as claimed in any one of claims 1 to 7, characterized in that: The following steps are involved: Thiamine, light calcium carbonate, magnesium oxide and sodium bicarbonate are mixed evenly, and then the composite bacterial agent capsule is added and mixed evenly to obtain a composition.

9. A feed additive, characterized in that: The feed additive comprises the sustained-release composition for solving rumen acidosis in ruminants as described in any one of claims 1 to 4.

10. A feed, characterized in that: The feed comprises the sustained-release composition for solving rumen acidosis in ruminants as described in any one of claims 1 to 4.