Root-source anti-acne composition specially researched for teenagers and application of root-source anti-acne composition

Abstract

The invention relates to a root-source anti-acne composition specially researched for teenagers and application thereof, the composition comprises Asiatic pennywort herb and fig fruit extract, and the Asiatic pennywort herb comprises Asiatic pennywort herb extract, asiaticoside and madecassoside. Compared with single centella asiatica, triple centella asiatica has the advantages that the soothing effect is stronger, the effects of controlling oil, removing acnes and the like are more comprehensive, the fig fruit extract can mildly dredge pores and accelerate skin renewal, and the fig fruit extract and triple centella asiatica have a certain synergistic effect on the anti-acne effect.

Description

Technical Field

[0001] The invention belongs to the technical field of skin care products, and relates to a composition specially designed for root-cause anti-acne in adolescents and its application. Background Art

[0002] With the sudden increase in academic and growth pressure, changes in hormone levels during adolescence, unhealthy eating habits high in oil, salt and sugar, and complicated lifestyles, the skin health problems of teenagers such as oil, acne, and sensitivity are becoming increasingly prominent, showing a trend of younger acne and lack of scientific care during adolescence. The main manifestations are increased secretion of sebaceous glands and active proliferation of keratinocytes, which lead to frequent and repeated oily skin, blackheads, and acne. The skin problems faced by teenagers seriously affect their self-confidence. There are many acid-containing acne and oil-control products on the market. The main function of acid-containing products is to accelerate epidermal metabolism and promote keratin exfoliation. Improper use of acid can easily lead to problems such as thinning of the stratum corneum, dryness and desquamation, and damaged skin barrier. For adults with a healthy skin barrier, the proper and correct use of acid can help dredge the cuticle and improve closed acne. However, teenagers have relatively weak skin and little experience in skin care. The use of acid products can easily cause problems such as thinning of the stratum corneum and damaged skin barrier.

[0003] Therefore, there is an urgent need to provide a composition specially developed for teenagers to fight acne from the root, to provide teenagers with a gentle acne removal solution, to stabilize the skin barrier, and to improve the skin condition from the bottom root. Summary of the invention

[0004] In view of the deficiencies of the prior art, the object of the present invention is to provide a composition and application thereof for adolescents to fight acne at the root cause.

[0005] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0006] In a first aspect, the present invention provides a composition for root-cause anti-acne for teenagers, the composition comprising triple Centella asiatica and fig fruit extract, wherein the triple Centella asiatica comprises Centella asiatica extract, asiatica glycoside and hydroxy-madecassoside.

[0007] Compared with single Centella asiatica, triple Centella asiatica has a stronger soothing effect, and its oil control, acne removal and soothing effects are more comprehensive. Among them, Centella asiatica extract can regulate excessive oil secretion, balance water and oil, inhibit lipid peroxidation, reduce sebum blockage and form acne closure. It contains a variety of active ingredients, which can inhibit Staphylococcus aureus and other bacteria related to acne pustules, and play an antibacterial and acne removal effect; hydroxy Centella asiatica glycoside can soothe redness and prevent red acne marks on the skin; Centella asiatica glycoside can promote healing and repair damaged acne skin. The three ingredients can cooperate with each other in oil control, acne removal and soothing effects. Fig fruit extract can gently unclog pores and accelerate skin renewal. It will not cause friction damage like physical exfoliation, nor will it cause irritation due to chemical exfoliation. It is gentle and efficient. In addition, this combination can promote skin healing, inhibit inflammatory factors, improve red acne marks, and repair the skin barrier to prevent repeated cycles of acne. Triple Centella asiatica and fig fruit extract also have a certain synergistic effect in the above effects.

[0008] Preferably, the composition comprises 0.01-20 parts of Centella asiatica and 0.001-5 parts of Ficus carica fruit extract by mass.

[0009] The mass fraction of triple Centella asiatica can be selected as 0.01 parts, 0.02 parts, 0.05 parts, 0.08 parts, 0.1 parts, 0.2 parts, 0.5 parts, 0.8 parts, 1 parts, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, 16 parts, 17 parts, 18 parts, 19 parts, 20 parts and the like; the mass fraction of fig fruit extract can be selected as 0.001 parts, 0.005 parts, 0.01 parts, 0.05 parts, 0.08 parts, 0.1 parts, 0.5 parts, 1 parts, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts and the like; other specific point values ​​within the above numerical range can be selected, which will not be repeated here one by one.

[0010] Preferably, the triple Centella asiatica comprises, by mass, 0.1-10 parts of Centella asiatica extract, 1-20 parts of asiatica glycoside, and 0.1-5 parts of madecassoside.

[0011] The mass fraction of Centella asiatica extract can be selected from 0.1 parts, 0.2 parts, 0.5 parts, 0.8 parts, 1 parts, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, etc., the mass fraction of asiatica glycoside can be selected from 1 parts, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, 16 parts, 17 parts, 18 parts, 19 parts, 20 parts, etc., the mass fraction of hydroxymadecassoside can be selected from 0.1 parts, 0.2 parts, 0.5 parts, 0.8 parts, 1 parts, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, etc., and other specific point values ​​within the above numerical range can be selected, which will not be repeated here one by one.

[0012] In a second aspect, the present invention provides a use of the composition for root-cause anti-acne for teenagers according to the first aspect in skin care products.

[0013] Preferably, the amount of the composition for root-cause anti-acne for teenagers added to skin care products is 0.01-25%, for example 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.1%, 0.12%, 0.15%, 0.18%, 0.2%, 0.5%, 1%, 1.2%, 1.5%, 1.8%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, 6%, 8%, 10%, 12%, 15%, 18%, 20%, 22%, 25%, etc. Other specific point values ​​within the above numerical range can be selected, which will not be repeated here.

[0014] In a third aspect, the present invention provides a skin care essence water specially developed for root-cause anti-acne treatment for teenagers, the skin care essence water comprising the composition specially developed for root-cause anti-acne treatment for teenagers as described in the first aspect, a moisturizer, a thickener, an antioxidant, a chelating agent and water.

[0015] Preferably, the skin care essence water comprises, by mass percentage, 0.01-25% of the root-cause anti-acne composition for teenagers described in the first aspect, 0.5-15% of a moisturizer, 0.01-5% of a thickener, 0.1-1% of an antioxidant, 0.01-0.1% of a chelating agent and 70-95% of water.

[0016] The mass percentage of the composition can be selected from 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.1%, 0.12%, 0.15%, 0.18%, 0.2%, 0.5%, 1%, 1.2%, 1.5%, 1.8%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, 6%, 8%, 10%, 12%, 15%, 18%, 20%, 22%, 25% and the like; the mass percentage of the humectant can be selected from 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, 2%, 3%, 5%, 8%, 10%, 12%, 15% and the like; the mass percentage of the thickener can be selected from 0.01%, 0.02%, 0.05%, 0.08%, 0.1%, 0.2%, 0.5%, 0 .8%, 1%, 1.2%, 1.5%, 1.8%, 2%, 2.2%, 2.5%, 2.8%, 3%, 3.2%, 3.5%, 3.8%, 4%, 4.2%, 4.5%, 4.8%, 5% and the like; the mass percentage of antioxidant can be selected as 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1% and the like; the mass percentage of chelating agent can be selected as 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.1% and the like; the mass percentage of water can be selected as 70%, 72%, 75%, 78%, 80%, 82%, 85%, 88%, 90%, 92%, 95% and the like; other specific point values ​​within the above numerical range can be selected, which will not be elaborated here one by one.

[0017] Preferably, the humectant includes any one of glycerol, butylene glycol or 1,2-hexanediol, or a combination of at least two thereof.

[0018] Preferably, the antioxidant comprises p-hydroxyacetophenone.

[0019] Preferably, the thickener comprises xanthan gum and / or sclerotium gum.

[0020] Preferably, the chelating agent comprises EDTA-disodium.

[0021] In a fourth aspect, the present invention provides a method for preparing the youth-specific root anti-acne skin care essence water according to the third aspect, the preparation method comprising:

[0022] (1) mixing a moisturizer and an antioxidant, and then mixing with a thickener, water, and a chelating agent, and homogenizing to obtain a first mixture;

[0023] (2) Mix the first mixture with the root anti-acne composition to obtain the product.

[0024] Preferably, the mixing temperature in step (1) is independently 80-85° C., and the mixing time is independently 8-12 min.

[0025] The temperature can be selected as 80°C, 81°C, 82°C, 83°C, 84°C, 85°C, etc., and the time can be selected as 8 min, 8.5 min, 9 min, 9.5 min, 10 min, 10.5 min, 11 min, 11.5 min, 12 min, etc. Other specific point values ​​within the above numerical range can be selected, and they will not be described here one by one.

[0026] Preferably, the homogenization speed is 3000-4000 rpm and the time is 3-5 min.

[0027] The rotation speed can be selected as 3000 rpm, 3100 rpm, 3200 rpm, 3300 rpm, 3400 rpm, 3500 rpm, 3600 rpm, 3700 rpm, 3800 rpm, 3900 rpm, 4000 rpm, etc., and the time can be selected as 3 min, 3.2 min, 3.5 min, 3.8 min, 4 min, 4.2 min, 4.5 min, 4.8 min, 5 min, etc. Other specific point values ​​within the above numerical range can be selected, and they will not be described here one by one.

[0028] Preferably, the first mixture is mixed with the root anti-acne composition at a temperature of 30-45° C. and a time of 5-8 min.

[0029] The temperature can be selected at 30°C, 32°C, 35°C, 38°C, 40°C, 42°C, 45°C, etc., and the time can be selected at 5 min, 5.5 min, 6 min, 6.5 min, 7 min, 7.5 min, 8 min, etc. Other specific point values ​​within the above numerical range can be selected, and they will not be described here one by one.

[0030] Compared with the prior art, the present invention has the following beneficial effects:

[0031] Compared with single Centella asiatica, triple Centella asiatica has a stronger soothing effect, and its oil control, acne removal and soothing effects are more comprehensive. Among them, Centella asiatica extract can regulate excessive oil secretion, balance water and oil, inhibit lipid peroxidation, reduce sebum blockage and form acne closure. It contains a variety of active ingredients, which can inhibit Staphylococcus aureus and other bacteria related to acne pustules, and play an antibacterial and acne removal effect; hydroxy Centella asiatica glycoside can soothe redness and prevent red acne marks on the skin; Centella asiatica glycoside can promote healing and repair damaged acne skin. The three ingredients can cooperate with each other in oil control, acne removal and soothing effects. Fig fruit extract can gently unclog pores and accelerate skin renewal. It will not cause friction damage like physical exfoliation, nor will it cause irritation due to chemical exfoliation. It is gentle and efficient. In addition, this combination can promote skin healing, inhibit inflammatory factors, improve red acne marks, and repair the skin barrier to prevent repeated cycles of acne. Triple Centella asiatica and fig fruit extract also have a certain synergistic effect in the above effects. DETAILED DESCRIPTION

[0032] In order to further explain the technical means and effects adopted by the present invention, the technical solution of the present invention is further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0033] The sources of the functional ingredients contained in the products involved in the following embodiments and comparative examples are as follows (only the functional ingredients are reflected, and the necessary auxiliary ingredients contained in other commercially available raw materials are not repeated):

[0034] Centella asiatica was purchased from Guangxi Changzhou Company under the trade name Centella asiatica;

[0035] Madecassoside was purchased from Guangxi Changzhou Company under the trade name of Madecassoside;

[0036] Centella asiatica extract was purchased from Guangxi Changzhou Company under the trade name Centella asiatica extract;

[0037] Fig fruit extract was purchased from Weiming Shiguang Company under the trade name of Ficus carica proteinase extract;

[0038] Xanthan gum was purchased from CP Kelco under the trade name KELTROL® CG LAX-T XANTHAN GUM;

[0039] Sclerotium gum was purchased from Guangzhou Xinrou Biotechnology Co., Ltd. under the trade name AimGum SG.

[0040] Example 1

[0041] The present embodiment provides a composition for root-cause anti-acne for teenagers, wherein the composition comprises 10 parts of triple Centella asiatica and 3 parts of fig fruit extract in parts by mass, wherein the triple Centella asiatica comprises Centella asiatica extract, asiatica glycoside and hydroxy-madecassoside in a mass ratio of 3:8:2.

[0042] The preparation method thereof is as follows: physically mixing the preparation raw materials to obtain the product.

[0043] Example 2

[0044] The present embodiment provides a composition for root-cause anti-acne for teenagers, wherein the composition comprises, by mass, 1 part of triple Centella asiatica and 5 parts of fig fruit extract, wherein the triple Centella asiatica comprises Centella asiatica extract, asiatica glycoside and hydroxy-madecassoside in a mass ratio of 1:20:1.

[0045] The preparation method is similar to that of Example 1.

[0046] Example 3

[0047] The present embodiment provides a composition for root-cause anti-acne for teenagers, wherein the composition comprises 20 parts of triple Centella asiatica and 0.1 parts of fig fruit extract by mass, wherein the triple Centella asiatica comprises Centella asiatica extract, asiatica glycoside and hydroxy-madecassoside in a mass ratio of 10:1:5.

[0048] The preparation method is similar to that of Example 1.

[0049] Comparative Example 1

[0050] This comparative example provides a composition for root-cause anti-acne in adolescents, which differs from Example 1 only in that it does not contain asiatica glycoside, and its reduced mass is proportionally distributed to the mass of hydroxymadecassoside and Centella asiatica extract, while other ingredients and contents remain unchanged.

[0051] The preparation method is similar to that of Example 1.

[0052] Comparative Example 2

[0053] This comparative example provides a composition for root-cause anti-acne for teenagers, which differs from Example 1 only in that it does not contain hydroxymadecassoside, and its reduced mass is proportionally distributed to the mass of asiatica glycoside and Centella asiatica extract, while other ingredients and contents remain unchanged.

[0054] The preparation method is similar to that of Example 1.

[0055] Comparative Example 3

[0056] This comparative example provides a composition for root-cause anti-acne treatment for teenagers, which differs from Example 1 only in that it does not contain Centella asiatica extract, and its reduced mass is proportionally distributed to the mass of Centella asiatica and madecassoside, while other ingredients and contents remain unchanged.

[0057] The preparation method is similar to that of Example 1.

[0058] Comparative Example 4

[0059] This comparative example provides a composition for root-cause anti-acne for teenagers, which differs from Example 1 only in that it does not contain triple Centella asiatica, and its reduced mass is distributed to the mass of fig fruit extract, while other ingredients and contents remain unchanged.

[0060] The preparation method is similar to that of Example 1.

[0061] Comparative Example 5

[0062] This comparative example provides a composition for root-cause anti-acne for teenagers, which differs from Example 1 only in that it does not contain fig fruit extract, and its reduced mass is distributed to the mass of triple Centella asiatica, while other ingredients and contents remain unchanged.

[0063] The preparation method is similar to that of Example 1.

[0064] Application Example 1

[0065] This application example provides a skin care essence water specially developed for youth to fight acne from the root cause, and its composition is:

[0066]

[0067] Its preparation method is:

[0068] (1) mixing the moisturizer and antioxidant at 83° C. for 10 min, and then mixing with the thickener, water, and chelating agent at 83° C. for 10 min, and homogenizing at 3500 rpm for 4 min to obtain a first mixture;

[0069] (2) Mix the first mixture and the composition at 40° C. for 6 minutes to obtain the product.

[0070] Application Example 2

[0071] This application example provides a skin care essence water specially developed for youth to fight acne from the root cause, and its composition is:

[0072]

[0073] Its preparation method is:

[0074] (1) mixing the moisturizer and antioxidant at 80° C. for 12 min, and then mixing with the thickener, water, and chelating agent at 80° C. for 12 min, and homogenizing at 3000 rpm for 5 min to obtain a first mixture;

[0075] (2) Mix the first mixture and the composition at 30° C. for 8 minutes to obtain the product.

[0076] Application Example 3

[0077] This application example provides a skin care essence water specially developed for youth to fight acne from the root cause, and its composition is:

[0078]

[0079] Its preparation method is:

[0080] (1) mixing the moisturizer and antioxidant at 85° C. for 8 min, and then mixing with the thickener, water, and chelating agent at 85° C. for 8 min, and homogenizing at 4000 rpm for 3 min to obtain a first mixture;

[0081] (2) Mix the first mixture and the composition at 45° C. for 3 minutes to obtain the product.

[0082] Comparative Application Examples 1-5

[0083] This comparative application example provides 5 kinds of skin care essence water for root anti-acne in adolescents. The difference between it and application example 1 is that the composition of example 1 is replaced by the compositions of comparative examples 1-5 in equal amounts, and the contents of other ingredients remain unchanged.

[0084] The preparation method refers to Application Example 1.

[0085] Test Example 1

[0086] Anti-inflammatory and soothing effect test

[0087] Test method: Use 1 μg / mL of LPS to stimulate macrophages, and evaluate the soothing effect of the sample by detecting the changes in the content of pro-inflammatory factors (IL-1β, TNF-α) and inflammatory mediators (PGE2) after the sample is treated.

[0088] Positive control group: 0.01% dexamethasone

[0089] Samples to be tested: Examples 1-3, Comparative Examples 1-5

[0090] Specific steps:

[0091] (1) Cell seeding: 2.2×10 5The cells were inoculated into a 6-well plate at a seeding density of cells / well, 2 mL of cell suspension was added to each well, and the inoculated cell culture plate was placed in an incubator for further culture for 24 h (5% CO2, 37°C).

[0092] (2) Aspirate and discard the old cell culture medium in the wells. Add 1.8 mL of 1% sample aqueous solution to each well in the sample group, 1.8 mL of solvent control culture medium to each well in the negative control group, 1.8 mL of positive control culture medium to each well in the positive control group, and 1.8 mL of cell culture medium to each well in the blank control group.

[0093] (3) After administration, place the 6-well plate in a cell culture incubator (5% CO2, 37°C) for 2 h.

[0094] (4) LPS stimulation: 2 h after drug administration, 200 μL of the prepared LPS-containing working solution was added to each well of each group and the cells were placed in a cell culture incubator (5% CO2, 37°C) and cultured for 22 h.

[0095] (5) ELISA test: Collect the cell culture supernatant and perform ELISA test according to the instructions of the ELISA kit.

[0096]

[0097] Table 1

[0098]

[0099] It can be seen from the results in Table 1 that the composition prepared in the present application has good anti-inflammatory and soothing effects, and Centella asiatica extract, asiatica glycoside and hydroxy-madecassoside have a certain synergistic effect in the anti-inflammatory and soothing effects. Triple Centella asiatica has the best effect. Triple Centella asiatica and fig fruit extract can also cooperate with each other, and the effect is better when added at the same time.

[0100] Test Example 2

[0101] Acne-removing efficacy test

[0102] Samples to be tested: Application examples 1-3, comparative application examples 1-5

[0103] Eight groups of subjects with acne-prone skin were recruited, with 10 participants in each group, aged between 20 and 30 years old, with varying degrees of acne. The test areas were determined, and each test sample was used once a day for 7 days. The dosage among the subjects remained consistent, and the Antera 3D device was used to collect the changes in acne volume, surface area, and skin redness before (D0) and 7 days after (D7) using the product. The changes before and after use were compared, and the average test data was recorded in the table.

[0104]

[0105] Table 2

[0106]

[0107] It can be seen from the results in Table 2 that the skin care essence water containing the composition prepared by the present application has a good acne-removing effect, and Centella asiatica extract, asiatica glycoside and hydroxy-madecassoside have a certain synergistic effect in the acne-removing effect. Triple Centella asiatica has the best effect. Triple Centella asiatica and fig fruit extract can also cooperate with each other, and the effect is better when added at the same time.

[0108] Test Example 3

[0109] Oil control efficacy test

[0110] The German CK skin oil tester Sebumeter SM815 was used to evaluate the efficacy of oil absorption value. 80 testers with healthy facial skin aged 18-30 were selected and divided into 8 groups, with 10 people in each group.

[0111] Samples to be tested: Application examples 1-3, comparative application examples 1-5

[0112] The volunteers first cleaned their faces with the same cleanser, wiped them dry, and used the forehead as the test area. The test area was divided into a blank group (no sample was wiped) and a sample group. Before using the product, the oil content of the forehead was tested; then, the sample to be tested was applied evenly to the skin in the sample area. The amount used by each tester was kept consistent. The skin oil content of the blank group and the sample group on the forehead was tested at 2 hours and 8 hours. Then the average oil reduction rate of the sample group compared with the blank group was calculated, and the test results were recorded in the table:

[0113] The test results of the sample group at 2 h and 8 h were recorded as Ax, and the initial test result of the sample group was recorded as A0. The test results of the blank group at 2 h and 8 h were recorded as Bx, and the initial test result of the blank group was recorded as B0.

[0114]

[0115] Table 3

[0116]

[0117] It can be seen from the results in Table 3 that the skin care essence water containing the composition prepared by the present application has a good oil control effect, and Centella asiatica extract, asiatica glycoside and hydroxy-madecassoside have a certain synergistic effect in the oil control effect. Triple Centella asiatica has the best effect. Triple Centella asiatica and fig fruit extract can also cooperate with each other, and the effect is better when added at the same time.

[0118] Test Example 4

[0119] Safety experiments:

[0120] The samples of Application Examples 1-3 were taken for skin irritation test. 30 subjects were selected, and the patch test tape containing 0.03 mL of the sample was applied to the normal skin of the left and right forearms of the subjects. After the patch was removed, the test was carried out according to the CTFA Guide (1981) within half an hour, and a total of 6 times. On the 6th day after the skin was applied, an additional test was carried out to observe the delayed reaction. The identification criteria are shown in Table 4, and the results are shown in Table 5.

[0121] Table 4

[0122]

[0123] Table 5

[0124]

[0125] It can be seen from the results in Table 5 that the skin care essence water containing the composition prepared in the present application has high safety and will not cause allergic reactions.

[0126] Test Example 5

[0127] In vitro exfoliation test

[0128] 1. Experimental materials: Use pig skin. Make sure the skin sample is fresh and undamaged, and remove impurities such as subcutaneous fat.

[0129] 2. Samples to be tested: Examples 1-3, Comparative Examples 1-5.

[0130] 3. Experimental methods:

[0131] (1) Sample pretreatment: Rinse the ex vivo skin sample with PBS buffer to remove surface impurities and residual blood, and then use scissors to cut the skin into small pieces of appropriate size for subsequent operations.

[0132] (2) Exfoliation: Place the treated skin samples in different culture dishes. Keep the weight of the skin samples in each culture dish the same. Add an equal amount of 1% aqueous solution of the sample to be tested to the culture dish to fully immerse the skin sample in the reagent. At the same time, set up a control group and add an equal amount of PBS buffer. Incubate the culture dish at room temperature for 24 hours.

[0133] (3) Collection of stratum corneum shed proteins:

[0134] ①After incubation, use tape to gently stick to the skin surface, and then press it 3 times with your hands to ensure that the tape is in full contact with the skin to pick up the shed stratum corneum cells. Remove the tape, cut it into small pieces, place it in a centrifuge tube, and add an equal amount of PBS solution.

[0135] ②Cell disruption and protein extraction: Freeze the cells in the centrifuge tube containing the tape, and then put it into an ultrasonic disruptor for 20 minutes to fully disrupt the keratinocytes and release the proteins in them. After the ultrasonication, centrifuge to obtain the supernatant and transfer it to a new centrifuge tube. This supernatant is the sample containing the stratum corneum shed protein.

[0136] (4) Protein quantification: Use the BCA protein quantification kit to perform quantitative analysis on the collected protein samples. The specific operation is as follows:

[0137] (1) Drawing of standard curve: According to the instructions of the kit, prepare standard solutions of different concentrations with protein standards of known concentrations, add BCA working solution, measure the absorbance at a specific wavelength on an ELISA reader, and draw a standard curve with protein concentration as the horizontal axis and absorbance as the vertical axis.

[0138] (2) Sample determination: Take a sample containing stratum corneum exfoliated protein, add BCA working solution, and measure the absorbance under the same conditions. The protein concentration (mg / mL) in the sample can be found on the standard curve or calculated by the linear regression equation based on the measured absorbance value.

[0139] Protein content (mg) = protein concentration × protein sample volume

[0140] The protein shedding amount of the control group was 100%.

[0141]

[0142] Table 6

[0143]

[0144] It can be seen from the results in Table 6 that the composition prepared in the present application has a good exfoliating effect, and Centella asiatica extract, asiatica glycoside and hydroxy-madecassoside have a certain synergistic effect in the exfoliating effect. Triple Centella asiatica has the best effect. Triple Centella asiatica and fig fruit extract can also cooperate with each other, and the effect is better when added at the same time.

[0145] The applicant declares that the present invention uses the above-mentioned embodiments to illustrate a composition for root-cause anti-acne in adolescents and its application, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of various raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0146] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0147] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A composition for treating acne at the root of the disease for adolescents, characterized in that: The composition comprises triple centella asiatica and fig fruit extract, wherein the triple centella asiatica comprises centella asiatica extract, asiatica glycoside and madecassoside.

2. The composition for treating acne at the root of the disease in adolescents according to claim 1, characterized in that: The composition comprises 0.01-20 parts of Centella asiatica and 0.001-5 parts of fig fruit extract by weight.

3. The composition for treating acne at the root of the disease in adolescents according to claim 1, characterized in that: The triple Centella asiatica comprises, by weight, 0.1-10 parts of Centella asiatica extract, 1-20 parts of asiatica glycoside, and 0.1-5 parts of madecassoside.

4. Use of the composition for root-cause anti-acne for teenagers according to any one of claims 1 to 3 in skin care products.

5. The use according to claim 4, characterized in that: The composition specially formulated for root-cause anti-acne treatment for teenagers is added in skin care products in an amount of 0.01-25%.

6. A skin care essence water specially developed for youth to fight acne root, characterized in that: The skin care essence water comprises the composition for root-cause anti-acne for teenagers according to any one of claims 1 to 3, a moisturizer, a thickener, an antioxidant, a chelating agent and water.

7. The youth-specific root anti-acne skin care essence water according to claim 6, characterized in that: The skin care essence water comprises, by mass percentage, 0.01-25% of the composition for root-cause anti-acne for teenagers according to any one of claims 1 to 3, 0.5-15% of a moisturizer, 0.01-5% of a thickener, 0.1-1% of an antioxidant, 0.01-0.1% of a chelating agent, and 70-95% of water.

8. The youth-specific root anti-acne skin care essence water according to claim 6, characterized in that: The moisturizing agent includes any one of glycerin, butylene glycol or 1,2-hexanediol or a combination of at least two thereof.

9. The method for preparing the youth-specific root anti-acne skin care essence water according to any one of claims 6 to 8, characterized in that: The preparation method comprises: (1) mixing a moisturizer and an antioxidant, and then mixing with a thickener, water, and a chelating agent, and homogenizing to obtain a first mixture; (2) Mix the first mixture with the root anti-acne composition to obtain the product.

10. The preparation method according to claim 9, characterized in that: The first mixture is mixed with the root anti-acne composition at a temperature of 30-45° C. and a time of 5-8 minutes.

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