Nano carbon dot calcium-copper nutrient solution with nutrition and sterilization functions and preparation method of nano carbon dot calcium-copper nutrient solution

Abstract

The invention belongs to the field of agricultural fertilizers, and relates to a nano carbon dot calcium-copper nutrient solution with both nutrition and sterilization functions and a preparation method of the nano carbon dot calcium-copper nutrient solution. The method comprises the following steps: (1) adding citric acid and ethylenediamine into a reaction kettle, introducing CO2-removed water into the reaction kettle, and carrying out pressurization and heating reaction to prepare a solution A; (2) compounding calcium gluconate and urea according to a certain ratio, adding monoethanolamine, introducing CO2-removed water into a reaction kettle, and carrying out pressure-maintaining heating to prepare a solution B; (3) preparing a solution C: mixing alkyl glycoside, methyl oleate and fatty alcohol polyvinyl ether according to a certain proportion, introducing CO2-removed water, and continuously stirring to prepare the solution C; and (4) sequentially compounding the solution A, the solution B and copper glycinate, and adding the solution C to prepare a finished product of the nano carbon dot calcium-copper nutrient solution. The nutrient solution has multiple effects: organic nutrients are supplemented to crops; photosynthesis of crops is improved; the calcium absorption is increased; bacterial diseases are prevented; nutrient solution leaf attachment is increased, and the utilization rate is increased.

Description

Technical Field

[0001] The invention belongs to the technical field of agricultural fertilizers, and specifically relates to a nano-carbon dot calcium copper nutrient solution with both nutritional and bactericidal functions and a preparation method thereof. Background Art

[0002] The frequent occurrence of adverse environmental stresses such as low temperature, low light, flooding, drought, and bacterial infection restricts the healthy growth of crops. In severe cases, it leads to a decrease in crop yield and quality, and reduced farmers' income.

[0003] At present, the substances that regulate crop growth are basically divided into three categories: chemical nutrients, organic nutrients, and growth regulators. Chemical nutrients are mainly composed of nitrogen, phosphorus and potassium nutrients, which are used to supplement foliar nutrition; organic nutrients are mainly composed of amino acids, humic acid, and molasses substances, which mainly enhance the growth of crops; growth regulators, the mainstream ones are ampicillin, sodium nitrophenolate, etc., which mainly play a role in accelerating cell division and promoting growth.

[0004] Under adverse conditions, crops first synthesize nutrients through photosynthesis and carry out self-repair; secondly, they absorb trace elements, activate the activity of enzymes in the body, accelerate the distribution of nutrients in the body, and promote the coordinated growth of the aboveground and underground parts; thirdly, they stabilize the balance of electrolytes in the cytoplasm and increase the thickness of the cell wall to prevent infection by pathogens.

[0005] At present, there are few foliar fertilizers that mainly promote photosynthesis, increase cell wall thickness, and stabilize cell fluid concentration; chemical pesticides are still the main method for preventing diseases, but chemical pesticides have caused pesticide residues on fresh fruits and vegetables; at the same time, spraying nutrient solution alone on leaves with thick wax layers has poor adhesion and ductility, resulting in low utilization efficiency of nutrient solution, especially Ca 2+ , Cu 2+ Most nutrient elements enter cells through transport proteins, and their absorption efficiency is low, which leads to the overall unsatisfactory application effect of existing nutrient solution products. Summary of the invention

[0006] In view of the requirement that crops recover their growth and grow healthily under adverse stress, the present invention provides a nano-carbon dot calcium-copper nutrient solution with both nutritional and bactericidal functions and a preparation method thereof.

[0007] To achieve the above object, the present invention adopts the following technical solution: A method for preparing a nano-carbon dot calcium copper nutrient solution having both nutritional and bactericidal functions, the method comprising the following steps: (1) Preparation of Liquid A: Add citric acid and ethylenediamine into a reaction kettle, introduce de-CO2 water into the reaction kettle, pressurize and heat the reaction kettle to obtain a nanocarbon stock solution, i.e., Liquid A; (2) Preparation of Liquid B: Calcium gluconate and urea are compounded in a certain ratio, monoethanolamine is added, and de-CO2 water is introduced into a reactor with a shear stirring function, and the reaction is heated under pressure to obtain a nano-carbon dot calcium emulsion, i.e., Liquid B; (3) Preparation of liquid C: Alkyl glycoside, methyl oleate, and fatty alcohol polyethylene ether (AEO) are mixed in a certain proportion, de-CO2 water is introduced, and continuous stirring is performed to obtain a leaf attachment agent, namely liquid C; (4) After sequentially mixing the A solution and the B solution with copper glycinate, the C solution is added to prepare a finished product of the nano-carbon dot calcium-copper nutrient solution.

[0008] Furthermore, the raw materials used in the preparation process include, by weight: 40-120 parts of citric acid, 40-120 parts of ethylenediamine, 30-100 parts of calcium gluconate, 30-100 parts of urea, 15-100 parts of monoethanolamine, 30-100 parts of copper glycinate, 2-10 parts of alkyl glycoside, 5-20 parts of methyl oleate, 2-10 parts of fatty alcohol polyethylene ether (AEO), and 200-500 parts of deCO2 water.

[0009] Furthermore, the fatty alcohol polyvinyl ether (AEO) is one or more combinations of AEO-1 to AEO-9.

[0010] Furthermore, step (1) is specifically as follows: according to weight parts, 40-120 parts of citric acid and 40-120 parts of ethylenediamine are added into a reaction kettle, 100-250 parts of de-CO2 water are introduced into the reaction kettle, the pressure is increased to 1.8-5Mpa, the temperature is maintained at 170-180°C, the reaction is stirred for 2-3h, and the rotation speed is 100-500r / min to obtain liquid A.

[0011] Furthermore, step (2) is specifically as follows: according to parts by weight, 30-100 parts of calcium gluconate and 30-100 parts of urea are added to a reaction kettle, 100-250 parts of de-CO2 water are introduced, stirring at room temperature and pressure for 15-30 minutes, the rotation speed is 100-500r / min, 15-100 parts of monoethanolamine are introduced, the pressure is increased to 1.2-1.3MPa, the reaction temperature is controlled not to exceed 180°C, the pressure is maintained, the heating is stirred, and the reaction is reacted for 3-4 hours at a rotation speed of 100-300r / min to obtain liquid B.

[0012] Furthermore, step (3) is specifically as follows: according to parts by weight, 2-10 parts of alkyl glycoside, 5-20 parts of methyl oleate, and 2-10 parts of fatty alcohol polyvinyl ether are mixed in proportion, 10-50 parts of CO2-free water are added, and the mixture is dissolved and stirred for 20-40 minutes at a speed of >50 r / min to obtain liquid C. Specifically, in this step, if the production is carried out in winter, the mixture is heated to 40-60°C before stirring, and the mixture is dissolved and stirred at room temperature in other seasons.

[0013] Furthermore, step (4) is specifically as follows: evenly stir the obtained solution A, solution B and copper glycinate at room temperature and pressure, add solution C, mix thoroughly, cool to room temperature, and bottle to obtain the nano-carbon dot calcium copper nutrient solution.

[0014] A nano-carbon dot calcium-copper nutrient solution with both nutritional and bactericidal functions. The nano-carbon dot calcium-copper nutrient solution is prepared from the following raw materials in parts by weight: 40-120 parts of citric acid, 40-120 parts of ethylenediamine, 30-100 parts of calcium gluconate, 30-100 parts of urea, 15-100 parts of monoethanolamine, 30-100 parts of copper glycinate, 2-10 parts of alkyl glycoside, 5-20 parts of methyl oleate, 2-10 parts of fatty alcohol polyvinyl ether (AEO), and 200-500 parts of deCO2 water.

[0015] An application of a nano-carbon dot calcium-copper nutrient solution, which is used as a foliar nutrient solution.

[0016] Beneficial effects of the present invention: The nano-carbon dot calcium-copper nutrient solution provided by the present invention is a multi-effect foliar nutrient solution with the following five effects: (1) Supplement organic nutrition to crops and reduce the energy consumption of crop recovery; (2) Improve crop photosynthesis and increase the synthesis, transport and distribution of sugars; (3) Increase calcium absorption, stabilize cell fluid concentration, promote cell wall thickening, and promote healthy growth of crops; (4) Prevent bacterial diseases, reduce the use of chemical pesticides, and significantly improve fruit yield and quality (5) Increase the adhesion of nutrient solution to leaves and improve utilization rate. With strong adhesion and good penetration, the nutrient absorption rate of leaves is greatly improved. Through the above effects, the growth of crops can be quickly restored and the healthy growth of crops can be promoted. DETAILED DESCRIPTION

[0017] It should be noted that the following detailed descriptions are illustrative and are intended to provide further explanation of the present application. Unless otherwise specified, all technical and scientific terms used herein have the same meanings as those commonly understood by those skilled in the art to which the present application belongs.

[0018] In response to adverse stresses such as low temperature and low light, flooding and drought, and bacterial infection, it is generally believed that supplementing foliar nutrition can restore growth, but it ignores many problems. First, the supplemented nutrients are mostly inorganic salts rather than organic nutrients. Inorganic salts will damage the leaves, which will aggravate the damage to crops under adverse conditions, which is undoubtedly adding insult to injury; second, the supplemented nutrient solution must improve the photosynthesis of the plant itself and accelerate the migration and distribution of photosynthetic products, especially the transportation to the root system, enhance the vitality of the root system, and restore the root system's ability to absorb nutrients; third, under adverse stress, it is urgent to stabilize the concentration of cell sap, increase the thickness of cell walls, and prevent bacterial infection; fourth, increase the amount of nutrient solution hanging on the leaves and increase the utilization efficiency of the nutrient solution.

[0019] Based on this, the present invention provides a nano-carbon dot calcium-copper nutrient solution with both nutritional and bactericidal functions, which is prepared using the following raw materials: citric acid, ethylenediamine, calcium gluconate, monoethanolamine, urea, copper glycinate, alkyl glycoside, methyl oleate, fatty alcohol polyethylene ether (AEO), and de-CO2 water.

[0020] The invention uses citric acid and ethylenediamine to prepare a nano-carbon reserve solution, which promotes crop photosynthesis through energy transition and carbon dots capturing light energy, especially increasing light utilization efficiency under low-light conditions; simultaneously, calcium gluconate, urea and monoethanolamine are used to prepare a nano-carbon dot calcium emulsion under high-speed shearing and pressure-maintaining heating conditions, which increases the speed and amount of calcium entering cells, stabilizes the concentration of cell fluid, and promotes the synthesis of cell walls; furthermore, copper glycine is introduced to replace inorganic copper and chemical pesticides, thereby improving the bactericidal effect; the combination of the three types of substances, namely, the nano-carbon reserve solution, the nano-carbon dot calcium emulsion and the leaf attachment agent, is combined with an auxiliary agent with leaf surface attachment, extension and penetration effects, thereby greatly improving the utilization efficiency.

[0021] The present invention provides an embodiment of a method for preparing a nano-carbon dot calcium copper nutrient solution having both nutritional and bactericidal functions, the method comprising the following steps: (1) Preparation of Liquid A: Add citric acid and ethylenediamine into a reaction kettle, introduce de-CO2 water into the reaction kettle, pressurize and heat the reaction kettle to obtain a nanocarbon stock solution, i.e., Liquid A; (2) Preparation of Liquid B: Calcium gluconate and urea are compounded in a certain ratio, monoethanolamine is added, and de-CO2 water is introduced into a reactor with shear stirring function, and pressure is maintained and heated to obtain a nano-carbon dot calcium emulsion, i.e., Liquid B; the reactor used in this step is equipped with shear stirring function, and has pressure maintenance and heating function.

[0022] (3) Preparation of liquid C: Alkyl glycoside, methyl oleate, and fatty alcohol polyethylene ether (AEO) are mixed in a certain proportion, de-CO2 water is introduced, and continuous stirring is performed to obtain a leaf attachment agent, namely liquid C; (4) After sequentially mixing the A solution and the B solution with copper glycinate, the C solution is added to prepare a finished product of the nano-carbon dot calcium-copper nutrient solution.

[0023] In this embodiment, the raw materials used in the preparation process include, by weight: 40-120 parts of citric acid, 40-120 parts of ethylenediamine, 30-100 parts of calcium gluconate, 30-100 parts of urea, 15-100 parts of monoethanolamine, 30-100 parts of copper glycinate, 2-10 parts of alkyl glycoside, 5-20 parts of methyl oleate, 2-10 parts of fatty alcohol polyethylene ether (AEO), and 200-500 parts of deCO2 water.

[0024] In this embodiment, the fatty alcohol polyvinyl ether (AEO) is one or more combinations of AEO-1 to AEO-9.

[0025] In this embodiment, step (1) is specifically as follows: according to weight parts, 40-120 parts of citric acid and 40-120 parts of ethylenediamine are added to a reaction kettle, 100-250 parts of de-CO2 water are introduced into the reaction kettle, the pressure is increased to 1.8-5Mpa, the temperature is maintained at 170-180°C, the reaction is stirred for 2-3h, and the rotation speed is 100-500r / min to obtain liquid A.

[0026] In this embodiment, step (2) is specifically as follows: according to parts by weight, 30-100 parts of calcium gluconate and 30-100 parts of urea are added to a reaction kettle, 100-250 parts of de-CO2 water are introduced, stirring at room temperature and pressure for 15-30 minutes, the rotation speed is 100-500 r / min, 15-100 parts of monoethanolamine are introduced, the pressure is increased to 1.2-1.3 MPa, the reaction temperature is controlled not to exceed 180°C, the pressure is maintained, the heating is stirred, and the reaction is reacted for 3-4 hours at a rotation speed of 100-300 r / min to obtain liquid B.

[0027] In this embodiment, step (3) is specifically as follows: according to parts by weight, 2-10 parts of alkyl glycoside, 5-20 parts of methyl oleate, and 2-10 parts of fatty alcohol polyvinyl ether (AEO) are mixed in proportion, 10-50 parts of CO2-free water are added, and the mixture is dissolved and stirred for 20-40 minutes at a speed of >50 r / min to obtain liquid C. Specifically, in this step, if the production is carried out in winter, the mixture is heated to 40-60°C before stirring, and the mixture is dissolved and stirred at room temperature in other seasons.

[0028] In this embodiment, step (4) is specifically as follows: stirring the obtained solution A, solution B and copper glycinate evenly at room temperature and pressure, adding solution C, mixing thoroughly, cooling to room temperature, bottling, and obtaining a nano-carbon dot calcium copper nutrient solution.

[0029] The test materials used in the examples of the present invention are all conventional test materials in the art and can be purchased through commercial channels.

[0030] In order to enable those skilled in the art to more clearly understand the technical solution of the present application, the technical solution of the present application will be described in detail below in conjunction with specific embodiments.

[0031] Example 1: A method for preparing a nano-carbon dot calcium copper nutrient solution having both nutritional and bactericidal functions, the method comprising: Place 100 parts of citric acid (by weight, the same below) and 80 parts of ethylenediamine in a reaction kettle, introduce 220 parts of de-CO2 water, pressurize to 1.8Mpa, maintain the temperature at 170-180℃, stir and react for 2-3h at a speed of 100r / min to obtain liquid A.

[0032] Place 50 parts of calcium gluconate and 100 parts of urea in a reactor, introduce 150 parts of de-CO2 water, stir for 15-30 min at room temperature and pressure, and rotate at 100 r / min; after mixing, add 15 parts of monoethanolamine, pressurize to 1.2-1.3 MPa, maintain pressure, heat, stir and react for 3-4 h, control the reaction temperature not exceeding 180°C, and rotate at 300 r / min to obtain liquid B.

[0033] Add 25 parts of deCO2 water to 5 parts of alkyl glycoside, 10 parts of methyl oleate and 10 parts of fatty alcohol polyvinyl ether (AEO), dissolve and stir at room temperature for 20-40 minutes at a speed of >50r / min to obtain liquid C.

[0034] The above-mentioned liquid A and liquid B are completely mixed, 100 parts of copper glycinate are added, liquid C is added, and the mixture is cooled to room temperature to obtain a nano-carbon dot calcium copper nutrient solution having both nutritional and bactericidal functions.

[0035] In order to better verify the effect of the nano-carbon dot calcium-copper nutrient solution for low temperature and low light conditions described in this example, experiments were conducted on greenhouse cucumbers in autumn and winter with continuous rainy days.

[0036] (1) Test time and location Test location: Huangzhen, Linzi, the soil is moderately salinized tidal soil, basic soil nutrients, soil alkaline nitrogen 105g / kg, available phosphorus 45.3mg / kg, available potassium 279.35mg / kg, organic matter 18g / kg, pH 5.8. Continuous low temperature (-2℃-10℃). It was cloudy and rainy for 5 days, and the incidence of bacterial diseases was more than 85%.

[0037] Trial time: December 2023.

[0038] (2) Experimental design The experiment set up 3 treatments, treatment 1 was the nutrient solution prepared in Example, treatment 2 was commercial foliar fertilizer, and treatment 3 was the control group, sprayed with the same amount of clean water. (3) Results analysis The chlorophyll content and total iron content of cucumber leaves were tested, and the core quality of the fruit was statistically analyzed. The results are shown in Table 1, and the incidence statistics are shown in Table 2.

[0039] Table 1 Comparison of detection indicators

[0040] The results in Table 1 show that the application of the nutrient solution of the present invention increases the chlorophyll content of leaves by 44.8%, the calcium content of leaves by 45.2%, and the sugar content by 25.3% compared with the application of foliar fertilizer, and the quality is significantly improved. The chlorophyll content of leaves increases by 15.7%, the calcium content of leaves increases by 18.6%, and the sugar content increases by 6.3% compared with the commercially available foliar fertilizer.

[0041] Table 2 Disease index

[0042] The results in Table 2 show that: the application of the nutrient solution of the present invention reduces the incidence rate by 50% and the disease index by 3 times compared with the application of no foliar fertilizer; the application of the nutrient solution of the present invention reduces the incidence rate by 7.8% and the disease index by 69.6% compared with the application of commercially available foliar fertilizer, and the disease prevention effect is improved by 48.0%.

[0043] Example 2: A method for preparing a nano-carbon dot calcium copper nutrient solution having both nutritional and bactericidal functions, the method comprising: Place 100 parts of citric acid (by weight, the same below) and 60 parts of ethylenediamine in a reaction kettle, introduce 180 parts of de-CO2 water, pressurize to 1.8Mpa, maintain the temperature at 170-180℃, stir and react for 2-3h at a speed of 100r / min to obtain liquid A.

[0044] 50 parts of calcium gluconate and 100 parts of urea were placed in a reactor, 240 parts of de-CO2 water were introduced, and the mixture was stirred at room temperature and pressure for 15-30 minutes at a speed of 100 r / min. After the mixture was mixed, 80 parts of monoethanolamine were added, and the mixture was pressurized to 1.2-1.3 MPa, and the mixture was stirred and reacted under pressure for 3-4 hours. The reaction temperature was controlled not to exceed 180°C, and the rotation speed was 300 r / min to obtain liquid B. It should be noted that the reactor has a shear stirring function and is heated under pressure.

[0045] Add 50 parts of deCO2 water to 5 parts of alkyl glycoside, 15 parts of methyl oleate and 2 parts of fatty alcohol polyvinyl ether (AEO), dissolve and stir at room temperature for 20-40 minutes at a speed of >50r / min to obtain liquid C.

[0046] The above-mentioned liquid A and liquid B are completely mixed, 100 parts of copper glycinate are added, all of liquid C is added, and the mixture is cooled to room temperature to obtain a nano-carbon dot calcium copper nutrient solution having both nutritional and bactericidal functions.

[0047] In order to better verify the effect of the nano-carbon dot calcium-copper nutrient solution for disease conditions described in this example, a potted plant test method was used to conduct an experiment on tomatoes.

[0048] Experimental materials and methods (1) Test time and location Test location: Daiyue District, Tai'an City, Shandong Province.

[0049] Test method: pot test.

[0050] Trial time: December 2023.

[0051] (2) Experimental design Tomato seedlings with consistent growth and 2 leaves and 1 heart were selected and transplanted into pots with a diameter of 15 cm. The water and fertilizer management was kept at the same level for each treatment. Foliar spraying was performed after 3 weeks. Three treatments were designed. Treatment 1 was the nutrient solution prepared in the embodiment, diluted 500 times, treatment 2 was a commercially available conventional foliar fertilizer, and treatment 3 was an equal amount of clean water. After 24 hours of treatment, bacterial wilt was inoculated. The inoculation was carried out using a 1 mL syringe (without the needle) and inoculated into the tomato leaves and the aboveground leaves at 1-2 cm above the stem. The corresponding detection was started 15 days after the treatment.

[0052] The experiment was designed with three replications. Except for the different foliar fertilizers used, the growth and treatment conditions of the treatments were the same.

[0053] (3) Results analysis The chlorophyll content of tomato leaves was tested, and the results are shown in Table 3. The disease index statistics are shown in Table 4.

[0054] Table 3 Comparison of detection indicators

[0055] The results in Table 3 show that: the application of the nutrient solution of the present invention increases the chlorophyll a content of leaves by 61.59%, the chlorophyll b content by 61.36%, and the total chlorophyll content by 72.00% compared with the application of no foliar fertilizer, and the growth of plants is significantly better than that of the control group. Compared with the commercially available foliar fertilizer, the chlorophyll a content of leaves increases by 30.41%, the chlorophyll b content increases by 20.34%, and the total chlorophyll content increases by 33.78%.

[0056] Table 4 Disease index

[0057] The results in Table 4 show that: the disease index of the application of the nutrient solution of the present invention is reduced by 45.7 percentage points compared with the application of no foliar fertilizer, and is reduced by 24.8 percentage points compared with the commercially available foliar fertilizer, and the disease prevention effect is significant.

[0058] Example 3: A method for preparing a nano-carbon dot calcium copper nutrient solution having both nutritional and bactericidal functions, the method comprising: Place 100 parts of citric acid (by weight, the same below) and 80 parts of ethylenediamine in a reaction kettle, introduce 200 parts of de-CO2 water, pressurize to 1.8Mpa, maintain the temperature at 170-180℃, stir and react for 2-3h at a speed of 100r / min to obtain liquid A.

[0059] 50 parts of calcium gluconate and 100 parts of urea were placed in a reactor, 200 parts of de-CO2 water were introduced, and the mixture was stirred at room temperature and pressure for 15-30 minutes at a speed of 100 r / min. After the mixture was mixed, 60 parts of monoethanolamine were added, and the mixture was pressurized to 1.2-1.3 MPa, and the mixture was stirred and reacted under pressure for 3-4 hours. The reaction temperature was controlled not to exceed 180°C, and the rotation speed was 300 r / min to obtain liquid B. It should be noted that the reactor has a shear stirring function and is heated under pressure.

[0060] Add 45 parts of deCO2 water to 5 parts of alkyl glycoside, 13 parts of methyl oleate and 2 parts of fatty alcohol polyvinyl ether (AEO), dissolve and stir at room temperature for 20-40 minutes at a speed of >50r / min to obtain liquid C.

[0061] The above-mentioned liquid A and liquid B are completely mixed, 80 parts of copper glycinate are added, all of liquid C is added, and the mixture is cooled to room temperature to obtain a nano-carbon dot calcium copper nutrient solution having both nutritional and bactericidal functions.

[0062] In order to better verify the effect of the nano-carbon dot calcium-copper nutrient solution for drought conditions described in this example, a potted plant test method was used to conduct an experiment on tomatoes.

[0063] Experimental materials and methods (1) Test time and location Test location: Daiyue District, Tai'an City, Shandong Province.

[0064] Trial time: July 2023.

[0065] Test method: Pot test (2) Experimental design Select pepper seedlings with consistent growth and plant height and transplant them into pots with a diameter of 26 cm. Plant one pepper seedling in each pot. Five pots are planted in each treatment for sampling and yield determination. Maintain normal water supply before budding. Start water control after budding. The watering amount during the water control period is 50% of the normal watering amount.

[0066] Foliar fertilizer spraying was carried out once before budding and at the peak fruiting stage. Treatment 1 used the nutrient solution in this embodiment, treatment 2 used conventional commercially available foliar fertilizer, and treatment 3 used clear water as a control.

[0067] Except for the different foliar fertilizers used among the treatments, the other growth and treatment conditions were the same among the treatments.

[0068] (3) Results analysis The effects of different treatments on pepper physiological parameters, growth, yield and related indicators are shown in Tables 5, 6 and 7.

[0069] Table 5 Effects of different treatments on pepper physiological parameters

[0070] The results in Table 5 show that: the application of the nutrient solution of the present invention increases the chlorophyll, SOD, POD and CAT contents of leaves by 38.78%, 36.70%, 12.85% and 29.36% respectively, and reduces the malondialdehyde content by 17.89% compared with the leaves without foliar fertilizer; the chlorophyll, SOD, POD and CAT contents of leaves by 25.93%, 21.78%, 4.29% and 16.50% respectively, and reduces the malondialdehyde content by 13.43% compared with the leaves with commercially available foliar fertilizer, indicating that the nutrient solution prepared in the embodiment can significantly reduce the damage of pepper leaves to drought.

[0071] Table 6 Effects of different treatments on pepper growth

[0072] The results in Table 6 show that: the application of the nutrient solution of the present invention increases the plant height, stem diameter and leaf area by 13.87%, 25.63% and 17.48% respectively compared with the application of no foliar fertilizer; the plant height, stem diameter and leaf area increase by 5.27%, 6.44% and 10.08% respectively compared with the commercially available foliar fertilizer, which significantly alleviates the inhibition of pepper growth under drought adversity.

[0073] Table 7 Effects of different treatments on pepper yield-related indicators

[0074] The results in Table 7 show that: the application of the nutrient solution of the present invention increases the fruit longitudinal diameter, fruit transverse diameter, single fruit weight and single plant yield by 16.04%, 21.98%, 32.87% and 62.59% respectively compared with the application of no foliar fertilizer; the fruit longitudinal diameter, fruit transverse diameter, single fruit weight and single plant yield increase by 9.58%, 7.20%, 9.72% and 11.83% respectively compared with the commercially available foliar fertilizer, which significantly improves the yield of pepper under drought stress.

[0075] The nutrient solution provided by the present invention utilizes nano-carbon to improve the photosynthesis efficiency of leaves, the leaf adhesive increases the nutrient retention of leaves, and the nano-carbon dots calcium and copper change the conformation of the leaf phospholipid bilayer to increase the absorption and utilization of calcium and copper to promote the healthy growth of crops. The product is green, safe, low-cost, and suitable for promotion.

[0076] The above description is only the preferred embodiment of the present application and is not intended to limit the present application. For those skilled in the art, the present application may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A method for preparing a nano-carbon dot calcium copper nutrient solution having both nutritional and bactericidal functions, characterized in that: The method comprises the following steps: (1) Preparation of Solution A: Add citric acid and ethylenediamine into a reaction kettle, introduce de-CO2 water into the reaction kettle, pressurize and heat the reaction kettle to obtain a nanocarbon stock solution, i.e. Solution A; (2) Preparation of Liquid B: Calcium gluconate and urea are compounded in a certain ratio, monoethanolamine is added, and de-CO2 water is introduced into a reactor with a shear stirring function, and the reaction is heated under pressure to obtain a nano-carbon dot calcium emulsion, i.e., Liquid B; (3) Preparation of Liquid C: Alkyl glycoside, methyl oleate, and fatty alcohol polyethylene ether are mixed in a certain proportion, de-CO2 water is introduced, and the mixture is continuously stirred to obtain a leaf attachment agent, i.e., Liquid C; (4) After sequentially mixing the A solution and the B solution with copper glycinate, the C solution is added to prepare a finished product of the nano-carbon dot calcium-copper nutrient solution.

2. The method for preparing the nano-carbon dot calcium copper nutrient solution having both nutritional and bactericidal functions according to claim 1, characterized in that: Calculated by weight, the raw materials used in the preparation process include: 40-120 parts of citric acid, 40-120 parts of ethylenediamine, 30-100 parts of calcium gluconate, 30-100 parts of urea, 15-100 parts of monoethanolamine, 2-10 parts of alkyl glycoside, 5-20 parts of methyl oleate, 2-10 parts of fatty alcohol polyvinyl ether, 30-100 parts of copper glycinate, and 200-500 parts of deCO2 water.

3. The method for preparing the nano-carbon dot calcium copper nutrient solution having both nutritional and bactericidal functions according to claim 1, characterized in that: The fatty alcohol polyvinyl ether is one or more combinations of AEO-1 to AEO-9.

4. The method for preparing the nano-carbon dot calcium copper nutrient solution having both nutritional and bactericidal functions according to claim 1, characterized in that: Step (1) is specifically as follows: according to weight parts, 40-120 parts of citric acid and 40-120 parts of ethylenediamine are added into a reaction kettle, 100-250 parts of de-CO2 water are introduced into the reaction kettle, the pressure is increased to 1.8-5Mpa, the temperature is maintained at 170-180°C, the reaction is stirred for 2-3h, and the rotation speed is 100-500r / min to obtain liquid A.

5. The method for preparing the nano-carbon dot calcium copper nutrient solution having both nutritional and bactericidal functions according to claim 1, characterized in that: Step (2) is specifically as follows: according to parts by weight, 30-100 parts of calcium gluconate and 30-100 parts of urea are added into a reaction kettle, 100-250 parts of de-CO2 water are introduced, stirring for 15-30 minutes at room temperature and pressure, with a rotation speed of 100-500 r / min, 15-100 parts of monoethanolamine are introduced, the pressure is increased to 1.2-1.3 MPa, the reaction temperature is controlled not to exceed 180° C., the pressure is maintained, the mixture is heated and stirred for reaction for 3-4 hours, with a rotation speed of 100-300 r / min, to obtain liquid B.

6. The method for preparing the nano-carbon dot calcium copper nutrient solution having both nutritional and bactericidal functions according to claim 1, characterized in that: Step (3) is specifically as follows: according to weight parts, 2-10 parts of alkyl glycoside, 5-20 parts of methyl oleate, and 2-10 parts of fatty alcohol polyvinyl ether are mixed in proportion, 10-50 parts of deCO2 water are added, and the mixture is dissolved and stirred for 20-40 minutes at a speed of >50 r / min to obtain liquid C.

7. The method for preparing the nano-carbon dot calcium copper nutrient solution having both nutritional and bactericidal functions according to claim 1, characterized in that: Step (4) is specifically as follows: evenly stir the obtained solution A, solution B and copper glycinate at room temperature and pressure, add solution C, mix thoroughly, cool to room temperature, and bottle to obtain the nano-carbon dot calcium copper nutrient solution.

8. A nano-carbon dot calcium copper nutrient solution with both nutritional and bactericidal functions, characterized in that: The nano-carbon dot calcium-copper nutrient solution is prepared by the method described in any one of claims 1 to 7, and the nano-carbon dot calcium-copper nutrient solution is prepared using the following raw materials in parts by weight: 40-120 parts of citric acid, 40-120 parts of ethylenediamine, 30-100 parts of calcium gluconate, 30-100 parts of urea, 15-100 parts of monoethanolamine, 30-100 parts of copper glycinate, 2-10 parts of alkyl glycoside, 5-20 parts of methyl oleate, 2-10 parts of fatty alcohol polyvinyl ether (AEO), and 200-500 parts of deCO2 water.

9. An application of the nano-carbon dot calcium copper nutrient solution according to claim 8, characterized in that: The nano-carbon dot calcium-copper nutrient solution is used as a foliar nutrient solution.

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