High kestose co-production psicose preparation device and psicose co-production preparation method

Abstract

The invention relates to a high kestose co-production psicose preparation device and a high kestose co-production psicose preparation method, belongs to the technical field of functional sugar preparation, and particularly relates to a kestose product with the purity of 85% or above prepared by utilizing high sugar concentration conversion and controlling the ratio of sugar liquid components in the reaction process. By-products in the preparation process are recycled for co-production of rare sugar psicose, and meanwhile, fructose and psicose coupled immobilized column isomerization production is adopted for improving the yield of psicose. According to the technology adopted by the invention, the product component degradation and energy consumption loss in the kestose production process are reduced, the by-product value and the conversion rate in the psicose co-production process are improved, and the method is a method for efficiently co-producing functional sugar and rare sugar.

Description

Technical Field

[0001] The invention belongs to the technical field of functional sugar preparation, in particular to the technical field of high-cane trisaccharide co-production of psicose. Background Art

[0002] Kestose is a trisaccharide formed by connecting a fructose molecule to sucrose. It is an important component of oligofructose. Studies have found that it has more significant effects in anti-allergy, metabolic syndrome, improving insulin resistance, and regulating the intestine. Allergies are the sixth largest chronic disease in the world, and about one-fifth of the world's population is deeply troubled by them. Therefore, the market application prospects of kestose are very broad. D-psicose is a diastereomer at the C-3 position of fructose, with a molecular formula of C6H 12 O6, molecular weight is 180.156, white crystal, odorless, not easy to absorb moisture, melting point is 96℃, very soluble in water, sugar content is 70% of sucrose, calories are 0.3% of sucrose, with similar taste and volume characteristics to sucrose, and like sucrose, it can react with amino acids or proteins in food to produce Maillard reaction, and is the best substitute for sucrose in food, known as "low-calorie sucrose". In 2011, the U.S. Food and Drug Administration officially approved D-psicose as a GRAS food after confirming its safety, allowing it to be used in food, pharmaceutical preparations and dietary supplements.

[0003] CN112210576B discloses a high-purity sucrose trisaccharide and its preparation method, which mainly involves immobilizing fructosyltransferase, optimizing saccharification parameters, and obtaining a sucrose trisaccharide product with a purity of more than 85% after chromatographic separation of the saccharified liquid. The solid content of the saccharified liquid is 30-60%.

[0004] CN110564626A discloses an Aspergillus oryzae strain and its culture method and a method for preparing kestose. The strain is used to improve the ability of converting sucrose into kestose, and a kestose crude solution with a kestose content of more than 55% is obtained.

[0005] The present invention utilizes high sugar concentration conversion and the proportion of sugar solution components in the reaction process to control the chromatographic raw materials for purifying kestose trisaccharide, so that a kestose trisaccharide product with a high purity of more than 85% can be obtained in one chromatography; at the same time, the by-products of chromatographic separation are utilized to produce rare sugar allulose, thereby improving the utilization value of the by-products. Summary of the invention

[0006] The purpose of the present invention is to prepare a cane trisaccharide product with a purity of more than 85% by utilizing high sugar concentration conversion and controlling the proportion of sugar solution components in the reaction process, and to recycle the by-products of the preparation process to jointly produce the rare sugar allulose.

[0007] To achieve the above-mentioned purpose, the technical scheme adopted by the present invention is a high-fructose triose co-production psicose preparation device, comprising a fructose and psicose coupling isomerization column, the fructose and psicose coupling isomerization column comprising a fructose isomerization column, a psicose isomerization column 1, a psicose isomerization column 2, a feed pipe, a feed and discharge pipe 1, a feed and discharge pipe 2, a final discharge pipe, and a thermal insulation interlayer; the fructose isomerization column, the psicose isomerization column 1, and the psicose isomerization column 2 are all provided with the thermal insulation interlayer; the feed pipe is connected to the fructose isomerization column, the fructose isomerization column is connected to the psicose isomerization column 1 through the feed and discharge pipe 1, the psicose isomerization column 1 is connected to the psicose isomerization column 2 through the feed and discharge pipe 2, and the psicose isomerization column 2 is provided with a final discharge pipe, and the final discharge pipe is used to discharge a psicose crude liquid.

[0008] Furthermore, a high-sugar cane triose co-production device and a method for producing psicose co-production include the following preparation steps:

[0009] (1) Preparing a cane trisaccharide product: adding β-fructose transferase to saccharify high-sugar concentrated sucrose syrup to obtain a saccharified liquid, wherein the saccharified liquid contains oligofructose and cane trisaccharide, and after the enzyme is inactivated and purified, a high-purity cane trisaccharide liquid is collected by chromatography, and a cane trisaccharide chromatography separation byproduct is obtained; the high-purity cane trisaccharide liquid is decolorized, deionized, concentrated, and dried to obtain a high-purity cane trisaccharide product;

[0010] (2) Obtaining psicose crystals from the byproduct of chromatographic separation of cane triose: concentrating the byproduct of chromatographic separation of cane triose into solids, hydrolyzing the solids with sucrase to obtain a hydrolyzate, and entering the hydrolyzate into the fructose and psicose coupled immobilized isomerization column to obtain the psicose crude liquid, and the psicose crude liquid is decolorized, deionized, concentrated, and then chromatographically purified, and then refined and crystallized to obtain psicose crystals.

[0011] Furthermore, in the step (1), the saccharification conditions are: sucrose syrup solids 75-78%, pH 6-7, temperature 65-70°C, enzyme addition amount 1.2-2.0L / t dry basis, saccharification time 16-45h;

[0012] Furthermore, in the step (1), the saccharified liquid has an oligofructose content of 31-46%, wherein catechinose accounts for 88-96% of the total oligofructose.

[0013] Furthermore, in step (1), the decolorization is high temperature and high sugar concentration decolorization, the activated carbon is neutral carbon, the decolorization temperature is 75-85°C, and the solid content of the decolorization liquid is 45-55%.

[0014] Furthermore, in step (1), the centrifugation is a high-temperature, high-sugar-concentration cis-continuous centrifugation, the centrifugation temperature is 40-55° C., and the solid content of the centrifugation liquid is 45-55%.

[0015] Furthermore, in step (1), the purity of kestose in the high-purity kestose product is greater than 85%.

[0016] Furthermore, in step (2), the byproduct of the chromatographic separation of cane trisaccharide is concentrated to a solid content of 50-60%, the pH is adjusted to 5.5-6.0, sucrose hydrolase is added and then hydrolyzed at 55-60° C. to obtain a hydrolyzate, and the hydrolyzate is fed into a fructose and psicose coupling isomerization column.

[0017] Furthermore, the hydrolyzate is fed into the fructose isomerization column (1) through the feed pipe (4), the hydrolyzate is fed into the fructose isomerization column (1), the feed liquid is fed from the bottom of the psicose isomerization column (2) through the feed and discharge pipe (5) after exiting the fructose isomerization column (1), the feed liquid enters the psicose isomerization column (2), and the upper layer of the psicose isomerization column (2) is pressurized; after exiting the psicose isomerization column (2), the feed liquid enters the psicose isomerization column (3) through the feed and discharge pipe (6), the output of the psicose isomerization column (3) is a crude liquid containing psicose, and the crude liquid is pumped into the refining process through the final discharge pipe (7).

[0018] Furthermore, the hydrolyzate enters the fructose isomerization column 1 at a flow rate of 0.3-1.2 Bv / h of immobilized enzyme volume, enters the psicose isomerization column 2 at a flow rate of 0.1-1 Bv / h of immobilized enzyme volume, and enters the psicose isomerization column 3 at a flow rate of 0.5-2 Bv / h.

[0019] Furthermore, the upper layer of the Allulose isomerization column-2 is pressurized to 0.2-0.3 MPa;

[0020] Furthermore, in the step (2), the purity of psicose in the crude psicose liquid is greater than 23%, and the purity of psicose crystals is greater than 97%.

[0021] The beneficial effects of the present invention are:

[0022] (1) High sugar concentration conversion can directly utilize sucrose syrup for conversion, reducing the loss of steam and other substances during the dissolution of sucrose crystals. High sugar concentration conditions can also protect the enzyme from reacting at high temperatures. At the same time, the substrate utilization rate is increased, promoting the conversion to trisaccharides.

[0023] (2) The coupled isomerization column of fructose and allulose increased the yield of allulose and reduced repeated refining. Meanwhile, the continuous isomerization column of allulose increased the conversion rate of allulose.

[0024] (3) The whole process adopts high sugar concentration operation, which reduces the increase of color value and product decomposition caused by repeated concentration of the feed liquid.

[0025] (4) Recycling by-products increases their value and increases the source of scarce sugar raw materials. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 Schematic diagram of the structure of the fructose and psicose coupling isomerization column used in the present invention;

[0027] Among them: 1. Fructose isomerization column; 2. Allulose isomerization column 1; 3. Allulose isomerization column 2; 4. Feed pipeline; 5. Feed and discharge pipeline 1; 6. Feed and discharge pipeline 2; 7. Final discharge pipeline; 8. Insulation interlayer. DETAILED DESCRIPTION

[0028] In order to enable those skilled in the art to better understand the technical solutions in the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present invention.

[0029] The present invention provides a device for preparing high-fructose triose co-production of psicose and a method for preparing co-production of psicose. The present invention is further described below through specific examples, but the present invention is not limited thereto.

[0030] Embodiment 1:

[0031] (1) Obtaining a high-purity cane trisaccharide product: adding β-fructose transferase to saccharify high-sugar concentrated sucrose syrup; inactivating the enzyme in the saccharified liquid, refining it, and collecting the high-purity cane trisaccharide liquid through chromatographic separation; then decolorizing, demixing, concentrating, and drying the high-purity cane trisaccharide liquid to obtain a high-purity cane trisaccharide product, the specific steps are as follows:

[0032] Sucrose syrup with a solid content of 75%, adjusted to pH 6-7, saccharification temperature 68°C, saccharification with β-fructosyltransferase at an enzyme dosage of 1.5L / t dry basis, saccharification time of 30h, oligofructose content in saccharification liquid 42.14%, saccharide accounted for 92.11% of total oligofructose;

[0033] After the saccharification liquid is inactivated, the solid content is adjusted to 55% and the liquid is decolorized at 80°C for 1 hour. The decolorization is high-temperature and high-sugar concentrated decolorization. The activated carbon is neutral carbon. After filtration, the liquid is subjected to high-temperature and high-sugar cis-continuous ionization. The ionization temperature is 50°C and the solid content of the ionization liquid is 45%. After the ionization output is concentrated, a high-purity sugarcane trisaccharide liquid is collected by chromatography, and a chromatographic separation by-product of the sugarcane trisaccharide chromatography is obtained. The chromatographic separation by-product of the sugarcane trisaccharide includes fructose, glucose and sucrose. The high-purity sugarcane trisaccharide liquid is decolorized at 75°C for 1 hour and the ionization temperature is 51°C. After being concentrated and dried again, a high-purity sugarcane trisaccharide product is obtained. The sugarcane trisaccharide content in the high-purity sugarcane trisaccharide product is 89.02%. The chromatographic separation by-product of the sugarcane trisaccharide is recycled again.

[0034] (2) Chromatographic separation of cane trisaccharide by-products and co-production of allulose

[0035] The byproduct of chromatographic separation of cane triose is hydrolyzed by sucrase, and the hydrolyzed sugar solution is fed into a fructose and psicose coupled immobilized isomerization column to obtain a liquid containing psicose, and the liquid is decolorized, cross-linked, concentrated, and then chromatographically purified, refined, and crystallized to obtain psicose crystals; the specific operation is as follows:

[0036] The byproduct of the chromatographic separation of cane trisaccharide is concentrated to a solid content of 50%, the pH is adjusted to 5.5-6.0, sucrose hydrolase is added and then hydrolyzed at 55°C to obtain a hydrolyzate, the hydrolyzate is fed into a fructose and psicose coupling isomerization column, and combined with Figure 1 For explanation, the fructose and allulose coupling isomerization column includes a fructose isomerization column 1, a allulose isomerization column 2, a allulose isomerization column 3, a feed pipe 4, an inlet and outlet pipe 1 5, an inlet and outlet pipe 2 6, a final outlet pipe 7, and a thermal insulation interlayer 8; the fructose isomerization column 1, the allulose isomerization column 2, and the allulose isomerization column 2 3 are all provided with a thermal insulation interlayer 8, and the thermal insulation interlayer 8 is used to maintain the temperature; the feed pipe 4 is connected to the fructose isomerization column 1, the fructose isomerization column 1 is connected to the allulose isomerization column 2 through the inlet and outlet pipe 1 5, the allulose isomerization column 2 is connected to the allulose isomerization column 2 3 through the inlet and outlet pipe 2 6, and the allulose isomerization column 2 3 is provided with a final outlet pipe 7, and the final outlet pipe 7 is used to discharge the allulose crude liquid.

[0037] Combination Figure 1For explanation, the hydrolyzate enters the fructose isomerization column 1 through the feed pipe 4, and the flow rate of the hydrolyzate entering the fructose isomerization column 1 is 0.3 Bv / h immobilized enzyme volume. After the feed liquid exits the fructose isomerization column 1, it is fed from the bottom of the psicose isomerization column 2 through the feed inlet and outlet pipe 5. The feed liquid enters the psicose isomerization column 2 at a flow rate of 0.1 Bv / h immobilized enzyme volume. The upper layer of the psicose isomerization column 2 is pressurized, and the upper layer is pressurized to 0.2 MPa; the feed liquid exits the psicose isomerization column 1. 2 and then enter the psicose isomerization column 23 through the inlet and outlet pipe 26. The flow rate into the psicose isomerization column 23 is 0.5 Bv / h. The liquid discharged from the psicose isomerization column 23 is a crude liquid containing psicose. The psicose content in the crude liquid is 24.2%. The crude liquid is pumped into the refining process through the final outlet pipe 7. The crude liquid is decolorized at 73° C. for 1 h, the centrifugation temperature is 52° C., and the crude liquid is concentrated and purified by chromatography. After refining, psicose crystals are crystallized to obtain psicose crystals. The purity of the psicose is 98.34%.

[0038] The present invention realizes the co-production of allulose by reusing the byproduct of chromatographic separation of cane trisaccharide in a functional sugar production line.

[0039] Embodiment 2:

[0040] (1) Obtaining a high-purity cane trisaccharide product: adding β-fructose transferase to saccharify high-sugar concentrated sucrose syrup; inactivating the enzyme in the saccharified liquid, refining it, and collecting the high-purity cane trisaccharide liquid through chromatographic separation; then decolorizing, demixing, concentrating, and drying the high-purity cane trisaccharide liquid to obtain a high-purity cane trisaccharide product, the specific steps are as follows:

[0041] Sucrose syrup with a solid content of 78%, adjusted to pH 6-7, saccharification temperature 65°C, saccharification with β-fructose transferase at an enzyme dosage of 1.2L / t dry basis, saccharification time of 16h, oligofructose content in saccharification liquid 31%, saccharose accounts for 88% of total oligofructose;

[0042] After the saccharification liquid is inactivated, the solid content is adjusted to 45%, and the liquid is decolorized at 85°C for 1 hour. The decolorization is high-temperature and high-sugar concentrated decolorization, and the activated carbon is neutral carbon. After filtration, the liquid is subjected to high-temperature and high-sugar cis-continuous ionization. The ionization temperature is 40°C, and the solid content of the ionization liquid is 45%. After the ionization output is concentrated, a high-purity sugarcane trisaccharide liquid is collected by chromatography, and a chromatographic separation by-product of the sugarcane trisaccharide chromatography is obtained. The chromatographic separation by-product of the sugarcane trisaccharide includes fructose, glucose and sucrose. The high-purity sugarcane trisaccharide liquid is decolorized at 75°C for 1 hour and the ionization temperature is 51°C. After being concentrated and dried again, a high-purity sugarcane trisaccharide product is obtained. The sugarcane trisaccharide content in the high-purity sugarcane trisaccharide product is 90.02%. The chromatographic separation by-product of the sugarcane trisaccharide is recycled.

[0043] (2) Chromatographic separation of cane trisaccharide by-products and co-production of allulose

[0044] The separated liquid of the byproduct of the chromatographic separation of cane triose is hydrolyzed by sucrase, the hydrolyzed sugar liquid is fed into a fructose and psicose coupled immobilized isomerization column to obtain a liquid containing psicose, the liquid is decolorized, cross-linked, concentrated, and then chromatographically purified, refined, and crystallized to obtain psicose crystals; the specific operation is as follows:

[0045] The byproduct of the chromatographic separation of cane trisaccharide is concentrated to a solid content of 60%, the pH is adjusted to 5.5-6.0, sucrose hydrolase is added and then hydrolyzed at 60°C to obtain a hydrolyzate, and the hydrolyzate is fed into a fructose and psicose coupling isomerization column, the structure of the fructose and psicose coupling isomerization column being the same as that of Example 1;

[0046] Combination Figure 1 For explanation, the hydrolyzate enters the fructose isomerization column 1 through the feed pipe 4, and the flow rate of the hydrolyzate entering the fructose isomerization column 1 is 1.2 Bv / h immobilized enzyme volume. After the feed liquid exits the fructose isomerization column 1, it is fed from the bottom of the psicose isomerization column 2 through the feed inlet and outlet pipe 5. The feed liquid enters the psicose isomerization column 2 at a flow rate of 1 Bv / h immobilized enzyme volume. The upper layer of the psicose isomerization column 2 is pressurized, and the upper layer is pressurized to 0.3 MPa; the feed liquid exits the psicose isomerization column 1. 2 and then enter the psicose isomerization column 23 through the inlet and outlet pipe 26. The flow rate into the psicose isomerization column 23 is 2Bv / h. The liquid discharged from the psicose isomerization column 23 is a crude liquid containing psicose. The psicose content in the crude liquid is 26.2%. The crude liquid is pumped into the refining process through the final outlet pipe 7. The crude liquid is decolorized at 75°C for 1h again, the centrifugation temperature is 50°C, and it is concentrated and purified by chromatography. After refining, it is crystallized to obtain psicose crystals. The purity of the psicose is 99.34%.

[0047] Embodiment 3:

[0048] (1) Obtaining a high-purity cane trisaccharide product: adding β-fructose transferase to saccharify high-sugar concentrated sucrose syrup; inactivating the enzyme in the saccharified liquid, refining it, and collecting the high-purity cane trisaccharide liquid through chromatographic separation; then decolorizing, demixing, concentrating, and drying the high-purity cane trisaccharide liquid to obtain a high-purity cane trisaccharide product, the specific steps are as follows:

[0049] The solid content of sucrose syrup is 77%, the pH is adjusted to 6-7, the saccharification temperature is 70°C, β-fructose transferase is added at an enzyme dosage of 2.0L / t dry basis, the saccharification time is 45h, the oligofructose content in the saccharification liquid is 46%, and saccharide accounts for 96% of the total oligofructose; the optimal solution is: the oligofructose content in the saccharification liquid is 37-46%, and saccharide accounts for 92-96% of the total oligofructose;

[0050] After the saccharification liquid is inactivated, the solid content is adjusted to 55%, and the liquid is decolorized at 75°C for 1 hour. The decolorization is high-temperature and high-sugar concentrated decolorization, and the activated carbon is neutral carbon. After filtration, the liquid is subjected to high-temperature and high-sugar cis-continuous ionization. The ionization temperature is 55°C, and the solid content of the ionization liquid is 55%. After the ionization output is concentrated, a high-purity sugar cane trisaccharide liquid is collected by chromatography, and a chromatographic separation by-product of the sugar cane trisaccharide is obtained at the same time, wherein the chromatographic separation by-product of the sugar cane trisaccharide includes fructose, glucose and sucrose; the high-purity sugar cane trisaccharide liquid is decolorized at 75°C for 1 hour, and the ionization temperature is 51°C, and the high-purity sugar cane trisaccharide product is obtained after concentration and drying again. The sugar cane trisaccharide content in the high-purity sugar cane trisaccharide product is 89.02%; and the chromatographic separation by-product of the sugar cane trisaccharide is recycled.

[0051] (2) Chromatographic separation of cane trisaccharide by-products and co-production of allulose

[0052] The separated liquid of the byproduct of the chromatographic separation of cane trisaccharide is hydrolyzed by sucrase, and the hydrolyzed sugar liquid is fed into a fructose and psicose coupled immobilized isomerization column to obtain a liquid containing psicose, and the liquid is decolorized, cross-linked, concentrated, and then chromatographically purified, refined, and crystallized to obtain psicose crystals; the specific operation is as follows:

[0053] The byproduct of the chromatographic separation of cane trisaccharide is concentrated to a solid content of 50%, the pH is adjusted to 5.5-6.0, sucrose hydrolase is added and then hydrolyzed at 60° C. to obtain a hydrolyzate, and the hydrolyzate is fed into a fructose and psicose coupling isomerization column, the structure of the fructose and psicose coupling isomerization column being the same as that of Example 1;

[0054] Combination Figure 1 For explanation, the hydrolyzate enters the fructose isomerization column 1 through the feed pipe 4, and the flow rate of the hydrolyzate entering the fructose isomerization column 1 is 1.2 Bv / h immobilized enzyme volume. After the feed liquid exits the fructose isomerization column 1, it is fed from the bottom of the psicose isomerization column 2 through the feed inlet and outlet pipe 5. The feed liquid enters the psicose isomerization column 2 at a flow rate of 1 Bv / h immobilized enzyme volume. The upper layer of the psicose isomerization column 2 is pressurized, and the upper layer is pressurized to 0.3 MPa; after the feed liquid exits the psicose isomerization column 2 The liquid enters the psicose isomerization column 23 through the inlet and outlet pipe 26, and the flow rate into the psicose isomerization column 23 is 0.5 Bv / h. The liquid discharged from the psicose isomerization column 23 is a crude liquid containing psicose, and the psicose content in the crude liquid is 25.2%. The crude liquid is pumped into the refining process through the final outlet pipe 7. The crude liquid is decolorized at 73° C. for 1 h, the centrifugation temperature is 52° C., and the crude liquid is concentrated and purified by chromatography. After refining, psicose crystals are crystallized to obtain psicose crystals. The purity of the psicose is 98.34%.

[0055] Although the present invention has been described in detail by referring to the accompanying drawings and in combination with the preferred embodiments, the present invention is not limited thereto. Without departing from the spirit and essence of the present invention, a person of ordinary skill in the art may make various equivalent modifications or substitutions to the embodiments of the present invention, and these modifications or substitutions shall be within the scope of the present invention or any person skilled in the art who is familiar with the present invention may easily think of changes or substitutions within the technical scope disclosed by the present invention, and shall be within the scope of protection of the present invention. Therefore, the scope of protection of the present invention shall be subject to the scope of protection of the claims.

Claims

1. A device for preparing high-fructose triose co-production of psicose, comprising a fructose and psicose coupling isomerization column, characterized in that: The fructose and psicose coupling isomerization column comprises a fructose isomerization column (1), a psicose isomerization column 1 (2), a psicose isomerization column 2 (3), a feed pipeline (4), a feed and discharge pipeline 1 (5), a feed and discharge pipeline 2 (6), a final discharge pipeline (7), and a heat-insulating interlayer (8); the fructose isomerization column (1), the psicose isomerization column 1 (2), and the psicose isomerization column 2 (3) are all provided with the heat-insulating interlayer (8); The feed pipe (4) is connected to the fructose isomerization column (1), the fructose isomerization column (1) is connected to the psicose isomerization column (2) through the feed and discharge pipe (5), the psicose isomerization column (2) is connected to the psicose isomerization column (3) through the feed and discharge pipe (6), and the psicose isomerization column (3) is provided with a final discharge pipe (7), and the final discharge pipe (7) is used to discharge the psicose crude liquid.

2. The method for producing psicose by using a high-fructose cane triose co-production device as claimed in claim 1, characterized in that: The method comprises the following preparation steps: (1) Preparing a cane trisaccharide product: adding β-fructose transferase to saccharify high-sugar concentrated sucrose syrup to obtain a saccharified liquid, wherein the saccharified liquid contains oligofructose and cane trisaccharide, and after the enzyme is inactivated and purified, a high-purity cane trisaccharide liquid is collected by chromatography, and a cane trisaccharide chromatography separation byproduct is obtained; the high-purity cane trisaccharide liquid is decolorized, deionized, concentrated, and dried to obtain a high-purity cane trisaccharide product; (2) Obtaining psicose crystals from the byproduct of chromatographic separation of cane triose: concentrating the byproduct of chromatographic separation of cane triose into solids, hydrolyzing the solids with sucrase to obtain a hydrolyzate, and entering the hydrolyzate into the fructose and psicose coupled immobilized isomerization column to obtain the psicose crude liquid, and the psicose crude liquid is decolorized, deionized, concentrated, and then chromatographically purified, and then refined and crystallized to obtain psicose crystals.

3. The method for producing psicose by using a high-fructose cane triose co-production device as claimed in claim 2, characterized in that: In the step (1), the saccharification conditions are: sucrose syrup solids 75-78%, pH 6-7, temperature 65-70°C, enzyme addition amount 1.2-2.0 L / t dry basis, and saccharification time 16-45 h.

4. The method for producing psicose by using a high-fructose cane triose co-production device as claimed in claim 2, characterized in that: In the step (1), the saccharification liquid contains fructo-oligosaccharides of 31-46%, of which catechins account for 88-96% of the total fructo-oligosaccharides.

5. The method for producing psicose by using a high-fructose cane triose co-production device as claimed in claim 2, characterized in that: In step (1), the decolorization is high-temperature, high-sugar, concentrated decolorization, the activated carbon is neutral carbon, the decolorization temperature is 75-85°C, and the solid content of the decolorized liquid is 45-55%.

6. The method for producing psicose by using a high-fructose cane triose co-production device as claimed in claim 2, characterized in that: In step (1), the centrifugation is a high-temperature, high-sugar-concentration cis-continuous centrifugation, the centrifugation temperature is 40-55° C., and the solid content of the centrifugation liquid is 45-55%.

7. The method for producing psicose by using a high-fructose cane triose co-production device as claimed in claim 2, characterized in that: In step (1), the purity of kestose in the high-purity kestose product is greater than 85%.

8. The method for producing psicose by using a high-fructose cane triose co-production device as claimed in claim 2, characterized in that: In step (2), the byproduct of the chromatographic separation of cane trisaccharide is concentrated to a solid content of 50-60%, the pH is adjusted to 5.5-6.0, sucrose hydrolase is added, and the hydrolyzate is hydrolyzed at 55-60° C. to obtain a hydrolyzate, and the hydrolyzate is fed into a fructose and psicose coupling isomerization column.

9. The method for producing psicose by using a high-fructose cane triose co-production device as claimed in claim 8, characterized in that: The hydrolyzed liquid enters the fructose isomerization column (1) through a feed pipe (4), and the hydrolyzed liquid enters the fructose isomerization column (1). After the feed liquid exits the fructose isomerization column (1), it is fed from the bottom of the psicose isomerization column (2) through a first feed and discharge pipe (5), and the feed liquid enters the first psicose isomerization column (2). The upper layer of the first psicose isomerization column (2) is pressurized; after the feed liquid exits the first psicose isomerization column (2), it enters a second psicose isomerization column (3) through a second feed and discharge pipe (6), and the liquid discharged from the second psicose isomerization column (3) is a crude liquid containing psicose, and the crude liquid is pumped into a refining process through a final discharge pipe (7).

10. The method for producing psicose by using a high-fructose cane triose co-production device as claimed in claim 9, characterized in that: The flow rate of the hydrolyzate into the fructose isomerization column 1 is 0.3-1.2 Bv / h of immobilized enzyme volume, the flow rate into the psicose isomerization column 2 is 0.1-1 Bv / h of immobilized enzyme volume, and the flow rate into the psicose isomerization column 3 is 0.5-2 Bv / h.

11. The method for producing psicose by using a high-fructose cane triose co-production device as claimed in claim 9, characterized in that: The upper layer of the Allulose isomerization column-2 is pressurized to 0.2-0.3 MPa.

12. The method for producing psicose by using a high-fructose cane triose co-production device as claimed in claim 2, characterized in that: The purity of psicose in the crude psicose liquid in step (2) is greater than 23%, and the purity of psicose crystals is greater than 97%.

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