Pleurotus citrinopileatus strain for producing ergothioneine, and preparation method and application of ergothioneine
By using specific elm elm mushroom strains and optimized fermentation conditions, the yield of ergothionein is improved, the problem of low ergothionein content in the prior art is solved, efficient production is achieved and its application potential in drug development is demonstrated.
Patent Information
- Application Number
- CN202411904102.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-23
- Publication Date
- 2025-03-28
AI Technical Summary
In the prior art, the ergothione content in the deep fermentation broth of edible fungi is low, and the strains with high yield of ergothione are still to be further developed, which limits its application potential in the fields of food additives, health products, etc.
The specific elm elm mushroom strain (CGMCC No. 41349) was used for liquid fermentation, using lactose and casein peptone as carbon and nitrogen sources, and the precursor methionine was added during the fermentation process, combining power ultrasound or repeated freeze-thaw assisted heat extraction to increase the yield of ergothionine.
The production of ergothionine was significantly improved. The production of ergothionine in strain 1 of yammoniae strain 1 reached 223.638ug/ml, which was used to alleviate the oxidative stress damage of HepG2 in liver cancer cells, demonstrating its potential in drug development.