Reaction system of abrus precatorius toxin, kit and method for detecting abrus precatorius toxin by utilizing LC-MS / MS (Liquid Chromatography-Mass Spectrometry / Mass Spectrometry)
By using single nucleotides as the detection substrate in the detection of acacia cotoxin, the reaction system and LC-MS/MS detection method are solved, and the problem of rapid and reliable detection of acacia cotoxin in the prior art is solved, and a high sensitivity and specific detection effect is achieved.
CN119738486AActive Publication Date: 2025-04-01ACADEMY OF MILITARY MEDICAL SCIENCES
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Patent Information
- Application Number
- CN202411595178.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-08
- Publication Date
- 2025-04-01
- Estimated Expiration
- 2044-11-08
AI Technical Summary
Technical Problem
The prior art is difficult to detect Acacia subtoxin quickly and reliably, especially when its extremely toxicity and ease of access make it a biological threat.
Method used
A Acacia toxin reaction system including buffer, single nucleotide substrate, BSA solution and ammonia water is adopted. Through the LC-MS/MS detection method, single nucleotides are used as detection substrate to detect the content of the adenine in the reaction to determine the presence of toxins.
Benefits of technology
High sensitivity and specific detection of acacia subtoxins is achieved, reducing detection costs and does not cross-react with other toxin detections.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention provides a reaction system and a kit of abrus precatorius toxin and a method for detecting the abrus precatorius toxin by utilizing LC-MS / MS (Liquid Chromatography-Mass Spectrometry / Mass Spectrometry), and belongs to the technical field of toxin detection. The method comprises the following steps: mixing and incubating a mononucleotide substrate, a buffer solution, a BSA solution and a to-be-detected sample, and adding ammonia water to terminate the reaction to obtain a reaction solution; and carrying out LC-MS / MS detection, and substituting the adenine peak area into the standard curve to calculate the concentration of adenine. When the deadenine quantity of the to-be-detected sample is greater than the sum of the average value of the deadenine quantity of the negative control and three times of the standard deviation, the to-be-detected sample can be judged as a positive sample. According to the invention, the antibody-coated magnetic beads are used for detection after AT enrichment, and the sensitivity can reach 0.391 ng / mL. The detection method provided by the invention is strong in specificity and high in sensitivity, can only act on mononucleotide containing adenine, and does not generate cross reaction with other toxin detection.
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