Antigen detection target of enterocytozoon hepatopenaei, monoclonal antibody for detecting enterocytozoon hepatopenaei and application of monoclonal antibody
By developing the antigen detection target EHP00_19 and its monoclonal antibodies against EHP, the problem of long and high cost of detection of EHP in the prior art is solved, and rapid and accurate detection without the need for extraction of nucleic acids is achieved, with strong specificity and high sensitivity, which is suitable for the prevention and control of enteroplasmosis in shrimp hepatoli.
CN119780434APending Publication Date: 2025-04-08SOUTHWEST UNIV
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Patent Information
- Application Number
- CN202510045228.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-13
- Publication Date
- 2025-04-08
AI Technical Summary
Technical Problem
The prior art requires the extraction and amplification of nucleic acids when detecting shrimp hepatic enteroplasm (EHP). It takes a long time, is costly and is prone to aerosol contamination, making it difficult to achieve fast and accurate detection.
Method used
The antigen detection target EHP00_19 and its corresponding monoclonal antibodies were developed, and monoclonal antibodies with strong specificity and high sensitivity were screened through reverse screening technology to achieve rapid and accurate detection of EHP without the need for nucleic acid extraction.
Benefits of technology
It realizes rapid, accurate and specific detection of EHP, high sensitivity, and is suitable for the prevention and control of enteroplasmosis in shrimp hepatobili.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention belongs to the technical field of biological detection and antibody preparation, and particularly relates to an antigen detection target of enterocytozoon hepatopenaei, a monoclonal antibody for detecting enterocytozoon hepatopenaei and application of the monoclonal antibody. A reverse screening technical route is adopted, enriched EHP is adopted to directly immunize mice, then myeloma cells are fused with splenocytes of the immunized mice to prepare hybridoma cells, and monoclonal antibodies are screened; and carrying out immunoblotting detection on EHP total protein by using the screened monoclonal antibody, carrying out mass spectrometry on specific protein bands, and screening to obtain an antigen detection target EHP0019 of EHP. The EHP0019 is a dominant antigen of the EHP, the EHP can be rapidly, accurately and specifically detected by utilizing the monoclonal antibody for specifically detecting the EHP0019 without nucleic acid extraction and amplification, the specificity is high, the sensitivity is high, and a foundation is laid for prevention and control of the hepatopancreatic microsporidiosis.
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