Method for improving carbon sequestration capability of ecological restoration base material containing kitchen garbage by using arbuscular mycorrhiza

Abstract

The invention provides a base material and a method for improving the carbon sequestration capacity of an ecological restoration base material containing kitchen garbage through arbuscular mycorrhiza. The base material comprises a dry spraying ecological restoration base layer and a wet spraying surface layer. The surface layer is prepared from the following components in parts by weight: a kitchen waste ecological restoration base material carbon sequestration mixture A, planting soil, a soil adhesive, a water-retaining agent, biochar and a compound fertilizer; the base layer is prepared from the following components in parts by weight: a kitchen waste ecological restoration base material carbon sequestration mixture B, planting soil, a soil adhesive, 100-mesh corn straw, biochar and a compound fertilizer. According to the method, pollution of the kitchen garbage to the environment is effectively reduced, the resource value of rich nutrients in the kitchen garbage is fully played, the functions of organic acid generated after the kitchen garbage is decomposed and arbuscular mycorrhiza for adjusting osmotic equilibrium and the like are fully played, the soil structure of the saline-alkali area can be improved, the soil fertility is improved, and the soil quality is improved. The salt tolerance of the plant is improved while the alkaline environment is improved.

Description

Technical Field

[0001] The present invention relates to the field of slope ecological restoration, and specifically to a method for improving the carbon fixation capacity of an ecological restoration substrate containing kitchen waste by utilizing arbuscular mycorrhizae. Background Art

[0002] With the improvement of residents' living standards, kitchen waste has become an important part of solid waste. If not handled properly, kitchen waste may cause serious pollution to the environment. At present, the main treatment methods for kitchen waste are landfill or incineration, which fail to fully tap the rich organic matter resources inside. Resource treatment of kitchen waste can not only effectively reduce its negative impact on the environment, but also transform it into valuable resources, achieving a win-win situation for the economy and the environment. At the same time, ecological restoration in saline-alkali areas has always been a focus of environmental restoration. Its high salinity, alkaline environment and poor soil structure have greatly restricted the ecological restoration process.

[0003] Ecological restoration is an effective measure to increase carbon sinks in terrestrial ecosystems and an important means to alleviate global warming. When kitchen waste is added to the ecological restoration substrate, environmental pollution is reduced; the organic acids produced by its decomposition can neutralize part of the salt, improve soil structure, and increase its carbon fixation capacity. As an effective ecological restoration tool, arbuscular mycorrhizal fungi also play an important role in improving carbon fixation capacity by improving plant salt tolerance, secreting organic acids, and improving aggregate stability. The extensive hyphal network of arbuscular mycorrhizal fungi enhances the absorption of water and nutrients by plants, and also secretes exoenzymes and organic acids to provide suitable living conditions for decomposing bacteria; decomposing bacteria decompose kitchen waste, provide nutrients for the growth of plants and arbuscular mycorrhizae, and promote the growth of both; plants produce organic carbon sources through photosynthesis, which can provide nutrients for decomposing bacteria and arbuscular mycorrhizae, and improve soil structure to provide a good environment. Considering the synergistic effect between arbuscular mycorrhizal fungi, plants, and decomposing bacteria, the combined action of arbuscular mycorrhizal fungi and decomposing bacteria promotes the ecological restoration process and significantly improves the carbon fixation capacity of the ecological restoration substrate.

[0004] In summary, it is necessary to find a method to use arbuscular mycorrhizal fungi and decomposing bacteria to improve the carbon fixation capacity of the ecological restoration substrate mixed with kitchen waste in saline-alkali areas. Summary of the invention

[0005] In order to solve the current technical problems, the main purpose of the present invention is to provide a method for improving the carbon fixation capacity of ecological restoration substrates containing kitchen waste by utilizing arbuscular mycorrhizae. This method can not only realize the resource utilization of kitchen waste, but also further enhance the ecological restoration effect and improve the carbon fixation capacity of the ecological restoration substrate.

[0006] In order to achieve the above technical features, the object of the present invention is achieved as follows: a substrate for improving the carbon fixation capacity of an ecological restoration substrate containing kitchen waste using arbuscular mycorrhizae, comprising an ecological restoration substrate surface layer and an ecological restoration substrate base layer; The surface layer of the ecological restoration substrate is prepared by uniformly mixing the kitchen waste ecological restoration substrate carbon fixation mixture A, planting soil, soil adhesive, water retaining agent, biochar, compound fertilizer, humic acid, gypsum, bagasse powder and plant seeds in a certain weight ratio; The kitchen waste ecological restoration substrate carbon fixation mixture A is prepared by mixing arbuscular mycorrhizal fungi, fruit and vegetable kitchen waste, bean kitchen waste, Bacillus subtilis, and Aspergillus niger fungi according to a certain weight ratio; The ecological restoration substrate base is prepared by uniformly mixing kitchen waste ecological restoration substrate carbon fixation mixture B, planting soil, soil adhesive, water retaining agent, 100-mesh corn stalks, biochar, compound fertilizer, gypsum, and bagasse powder according to a certain weight ratio; The kitchen waste ecological restoration substrate carbon fixation mixture B is composed of kitchen waste ecological restoration substrate carbon fixation mixture B1 and B2, B1 and B2 are wrapped by a polyacrylic acid outer film and separated by a curved polyacrylic acid film inside the outer film; the kitchen waste ecological restoration substrate carbon fixation mixture B1 is prepared by mixing arbuscular mycorrhizal fungi, kitchen waste, potato dextrose agar, and corn flour agar in a certain weight ratio; the kitchen waste ecological restoration substrate carbon fixation mixture B2 is prepared by mixing Bacillus subtilis, white rot fungi, actinomycetes, potato dextrose agar, and corn flour agar in a certain weight ratio.

[0007] Preferably, the weight ratio of the kitchen waste ecological restoration substrate carbon fixation mixture A, planting soil, soil adhesive, water retaining agent, biochar, compound fertilizer, humic acid, gypsum, and bagasse powder in the surface layer of the ecological restoration substrate is 2-6:100:1-2:0.1-0.3:1-2:0.8-1.2:0.5-1:1-2:0.5-1.

[0008] Preferably, the weight ratio of arbuscular mycorrhizal fungi, fruit and vegetable kitchen waste, bean kitchen waste, Bacillus subtilis and Aspergillus niger in the kitchen waste ecological restoration substrate carbon fixation mixture A is 2-3:45-50:45-50:1-2:1-2.

[0009] Preferably, the weight ratio of the kitchen waste ecological restoration substrate carbon fixation mixture B, planting soil, soil adhesive, water retaining agent, corn straw with 100 mesh, biochar, compound fertilizer, gypsum, and bagasse powder in the ecological restoration substrate base layer is 4-6:100:2-4:0.1-0.3:6-8:2-3:4-6:1-2:0.5-1.

[0010] Preferably, the weight ratio of arbuscular mycorrhizal fungi agent, kitchen waste, potato glucose agar, and corn flour agar in the kitchen waste ecological restoration substrate carbon fixation mixture B1 is 1-2:10-14:4-5:4-5; Preferably, the weight ratio of Bacillus subtilis, white rot fungi, actinomycetes, potato dextrose agar and corn flour agar in the kitchen waste ecological restoration substrate carbon fixation mixture B2 is 1:1:1:6-8:6-8.

[0011] Preferably, the arbuscular mycorrhizal fungi are a 1:1 mixture of Glomus mosseae and Glomus intraradices.

[0012] Preferably, the planting soil is taken from the topsoil of 0-10 cm in the soil surface layer in saline-alkali areas, and the soil is air-dried and crushed, and passed through a 10 mm sieve; The soil adhesive is a mixture of cement, bentonite and gelatin in a ratio of 1:1:1; The water retaining agent is a mixture of polyvinyl amide and polymer hydrogel in a ratio of 1:1; The biochar is prepared by mixing mineral biochar and wood biochar that have passed a 200-mesh sieve in a ratio of 1:1.

[0013] Preferably, the compound fertilizer is composed of quick-acting fertilizer and slow-release fertilizer, wherein the quick-acting fertilizers N, P, and K are mixed in a ratio of ammonium sulfate: diammonium phosphate: potassium sulfate of 1-2:1-2:1-2, and the slow-release fertilizer is polyamino acid slow-release fertilizer.

[0014] Preferably, the plant seeds have a density of 10-30 grams per square meter, and the plant seeds are mixed with bermudagrass, alfalfa, ryegrass, magnolia, raspberry, and poplar in a ratio of 3:3:3:2:2:1; The kitchen waste is dried in advance and then crushed through a 1 mm sieve. The kitchen waste in the kitchen waste ecological restoration base material carbon fixation mixture B1 is also fermented.

[0015] On the other hand, the present invention provides a method for improving the carbon fixation capacity of the ecological restoration substrate containing kitchen waste in saline-alkali areas by using the substrate for improving the carbon fixation capacity of the ecological restoration substrate containing kitchen waste using arbuscular mycorrhizae, comprising the following steps: Step 1: preparing a kitchen waste ecological restoration substrate carbon fixation mixture B: mixing the treated kitchen waste, potato dextrose agar, corn flour agar and arbuscular mycorrhizal fungi in a set weight ratio to prepare B1, and mixing Bacillus subtilis, white rot fungi, actinomycetes, potato dextrose agar and corn flour agar in the above weight ratio to prepare B2, B1 and B2 are wrapped by a polyacrylic acid outer membrane and separated by a curved polyacrylic acid membrane inside the outer membrane; Step 2: Constructing an ecological restoration base: Evenly mix the kitchen waste ecological restoration base material carbon fixation mixture B prepared in step 1, planting soil, soil adhesive, 100-mesh corn stalks, biochar, and compound fertilizer according to the above weight parts, and use a dry spraying process to spray on the slope surface to form a base with a thickness of 8-10 cm; Step 3: preparing a kitchen waste ecological restoration substrate carbon fixation mixture A: mixing arbuscular mycorrhizal fungi, fruit and vegetable kitchen waste, bean kitchen waste, Bacillus subtilis, and Aspergillus niger fungi according to a set weight ratio; Step 4: Construct the ecological restoration surface layer: Mix the kitchen waste ecological restoration substrate carbon fixation mixture A prepared in step 3 with planting soil, soil adhesive, water retaining agent, biochar, compound fertilizer and plant seeds in a set weight ratio, and use the wet spraying process to spray it on the base to form a surface layer with a thickness of 2-3 cm.

[0016] The present invention has the following beneficial effects: 1. The present invention adds humic acid to the surface layer to achieve the following beneficial effects: (1) Promote seed germination: It can stimulate the synthesis of plant hormones such as auxin and cytokinin in seeds, thereby achieving the purpose of promoting seed germination and seedling growth; (2) Improve saline-alkali land: Humic acid can reduce the pH of saline-alkali land, and at the same time, the organic acid in humic acid can combine with salts such as sodium ions in the soil to form a soluble complex, thereby improving saline-alkali land; (3) Accelerate the decomposition of kitchen waste: Humic acid can activate the microbial community in the soil, enhance the activity of decomposing bacteria, accelerate the decomposition of kitchen waste, convert it into a more stable organic carbon form, and release more nutrients; (4) Provide a growth environment: Humic acid can improve the soil aggregate structure, optimize the soil permeability and water retention capacity, and provide a suitable environment for plant germination root growth and microbial growth; (5) Weaken.

[0017] 2. The present invention adds gypsum to both the surface layer and the base layer, which has the following beneficial effects: (1) Improve the salt content in the soil: gypsum is rich in calcium sulfate, and calcium ions react with sodium ions in the soil to form soluble sodium sulfate, which can be washed away by precipitation, reducing the sodium ion concentration in the soil; (2) Accelerate the decomposition of kitchen waste: the calcium ions provided can promote microbial activity, promote the growth and metabolism of decomposing bacteria; (3) Improve soil structure: the calcium ions provided can combine with the negatively charged soil surface, increase the adsorption force between particles, improve soil structure, increase air permeability and water retention capacity, and provide a suitable environment for plant growth and microbial metabolism. (4) Reduce nutrient imbalance: kitchen waste contains a large amount of nitrogen and phosphorus nutrients, but lacks nutrients such as magnesium that are necessary for plant growth, which will affect ecological restoration. The increase in calcium ion concentration will cause magnesium ions to be replaced from soil particles for plant absorption.

[0018] 3. The present invention adds biochar to both the surface layer and the base layer, which has the following beneficial effects: (1) Adsorption of harmful gases: Biochar can alleviate harmful gases such as methane that may be produced by the decomposition of kitchen waste; (2) Providing a carbon source: Biochar provides a stable carbon source, promotes the accumulation of organic carbon in the soil, and helps to improve the soil's carbon fixation capacity; (3) Improving water retention capacity: The porous structure, high specific surface area and strong adsorption of biochar can significantly improve the soil's water retention capacity and promote microbial metabolism and plant growth.

[0019] 4. The present invention adds bagasse powder to both the surface layer and the base layer, which has the following beneficial effects: (1) Providing mineral elements such as magnesium and potassium to maintain the balance of nutrient elements in the soil: Gypsum is used to treat saline-alkali land, which may lead to excessive accumulation of calcium ions in the soil, weaken the plant's absorption of other important elements (potassium, magnesium), and cause malnutrition of plants. The above mineral elements in bagasse powder supplement magnesium and potassium fertilizers, which can appropriately alleviate the negative impact of excessive calcium ions on plant growth; (2) Accelerating the effect of gypsum on saline-alkali land: Gypsum dissolves slowly when the moisture content is low, and the treatment effect is poor, while bagasse powder can effectively enhance the water retention of the soil and accelerate the effect of gypsum on saline-alkali land; (3) Providing a carbon source: Bagasse powder, as a source of organic matter, promotes microbial activity to improve soil structure and effectively improves the soil's carbon sequestration capacity.

[0020] 5. The present invention directly stimulates the change of seed hormones and improves soil structure to increase moisture to promote seed germination by adding arbuscular mycorrhizal fungi to the ecological restoration surface layer; at the same time, indirectly enhances the decomposition ability of decomposing bacteria such as Bacillus subtilis and Aspergillus niger by secreting substances such as organic acids and improving the structure of soil aggregates, accelerates the decomposition of phosphorus-rich kitchen waste such as fruits, vegetables and beans, and produces phosphorus nutrients to promote seed germination.

[0021] 6. The present invention adopts an ecological restoration construction method combining dry spraying of the base layer and wet spraying of the surface layer. After the wet spraying of the surface layer, water penetrates into the base layer, so that the polyacrylic acid film wrapped around the carbon fixation mixture B of the kitchen waste ecological restoration substrate is dissolved. (1) Before the film is dissolved, the kitchen waste in the film can be used as a part of the culture medium to provide nutrition for arbuscular mycorrhizal fungi, which is convenient for the survival and preservation of arbuscular mycorrhizal fungi. (2) After the film is dissolved, 1) the kitchen waste in B1 is mixed with Bacillus subtilis, white rot fungi, and actinomycetes in B2, and the kitchen waste begins to decompose; 2) after the film is dissolved, it provides nutrient raw materials for decomposing bacteria and arbuscular bacilli, further accelerating the decomposition of kitchen waste; 3) after the film is dissolved, pores are formed in the ecological restoration substrate, providing space for the growth of decomposing bacteria, arbuscular mycorrhizal fungi and plant roots; 4) the curved surface structure of the partition membrane inside the soluble film makes the kitchen waste have more contact surface with the decomposing bacteria after dissolution, which can accelerate the decomposition process of the decomposing bacteria; 5) after the polyacrylic acid film is dissolved, the carboxyl group dissociates hydrogen ions, improving the soil pH.

[0022] 7. The present invention discloses a method for improving the carbon fixation capacity of a substrate for ecological restoration of kitchen waste in saline-alkali areas by combining arbuscular mycorrhizal fungi with decomposing bacteria. The combined action of arbuscular mycorrhizal fungi and decomposing bacteria in ecological restoration can achieve the following: (1) Improvement of soil structure: arbuscular mycorrhizal fungi increase the formation of soil aggregates through extensive hyphae, improve plant salt tolerance, secrete organic acids, improve the pH value, permeability and water retention capacity of saline-alkali soil, and are beneficial to the activity of microorganisms and the decomposition of organic matter, thereby promoting carbon fixation; (2) Promote the accumulation of organic matter: promote root growth, secrete organic matter, provide a carbon source for microbial growth, accelerate the decomposition of kitchen waste, and the produced organic acids can improve soil salinity and alkalinity, help improve the utilization rate of organic matter in kitchen waste by plants, and further improve the carbon fixation capacity of the soil; (3) Enhance plant growth resistance: symbiosis enhances the plant's ability to absorb water and nutrients, improves salt tolerance, promotes growth and photosynthesis, and increases carbon fixation.

[0023] 8. The present invention provides a method for improving the carbon fixation capacity of ecological restoration substrates mixed with kitchen waste in saline-alkali areas by combining arbuscular mycorrhizal fungi with decomposing bacteria, combining the principle of sustainable utilization of waste resources with ecological restoration technology, and applying it to ecological restoration substrates on the basis of daily kitchen waste treatment. The fertility of the ecological restoration substrate is increased, and the carbon fixation capacity of the ecological restoration substrate is enhanced, which has a good ecological restoration effect and realizes harmless and resourceful utilization. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0025] Figure 1 The figure is a flow chart of the preparation and construction of the substrate of the present invention.

[0026] Figure 2 It is a schematic diagram of the slope profile and the carbon fixation mixture of each layer of kitchen waste ecological restoration substrate of the present invention.

[0027] In the figure, 1 is a kitchen waste ecological restoration base material carbon fixation mixture A, 2 is a kitchen waste ecological restoration base material carbon fixation mixture B, 3 is a polyacrylic acid outer film, 4 is a curved polyacrylic acid inner film, 5 is a kitchen waste ecological restoration base material carbon fixation mixture B1, 6 is a kitchen waste ecological restoration base material carbon fixation mixture B2, 7 is arbuscular mycorrhizal fungi, 8 is kitchen waste, 9 is potato dextrose agar, 10 is corn flour agar, 11 is Bacillus subtilis, 12 is white rot fungi, 13 is actinomycetes, 14 is fruit and vegetable kitchen waste, 15 is bean kitchen waste, and 16 is Aspergillus niger. DETAILED DESCRIPTION

[0028] The embodiments of the present invention are further described below in conjunction with the accompanying drawings.

[0029] Embodiment 1 (minimum value): A method for improving the carbon fixation capacity of a food waste-mixed ecological restoration substrate in saline-alkali areas by combining arbuscular mycorrhizal fungi with decomposing bacteria comprises the following steps: Step 1: preparing a kitchen waste ecological restoration substrate carbon fixation mixture B: the kitchen waste ecological restoration substrate carbon fixation mixture B1 is mixed with arbuscular mycorrhizal fungi, kitchen waste, potato glucose agar, and corn flour agar in the ratio of 1.5:12:4.5:4.5, and the kitchen waste ecological restoration substrate carbon fixation mixture B2 is mixed with Bacillus subtilis, white rot fungi, actinomycetes, potato glucose agar, and corn flour agar in the ratio of 1:1:1:7:7, and B1 and B2 are wrapped by a polyacrylic acid outer film and separated by a curved polyacrylic acid film inside the outer film; Step 2: Constructing an ecological restoration base: Evenly mix the kitchen waste ecological restoration base material carbon fixation mixture B prepared in step 1, planting soil, soil adhesive, water retaining agent, 100-mesh corn stalks, biochar, compound fertilizer, gypsum, and bagasse powder according to 4:100:3:0.2:7:2.5:5:1.5:0.75 parts by weight, and spray onto the slope surface using a dry spraying process to form a 9 cm thick base; Step 3: preparing a kitchen waste ecological restoration substrate carbon fixation mixture A: mixing arbuscular mycorrhizal fungi, fruit and vegetable kitchen waste, bean kitchen waste, Bacillus subtilis, and Aspergillus niger fungi according to 2.5:47.5:47.5:1.5:1.5; Step 4: Construct an ecological restoration surface layer: Evenly mix the kitchen waste ecological restoration substrate carbon fixation mixture A prepared in step 3 with planting soil, soil adhesive, water retaining agent, biochar, compound fertilizer, humic acid, gypsum, bagasse powder and plant seeds in the ratio of 2:100:1.5:0.2:1.5:1:0.75:1.5:0.75 by weight, and then use a wet spraying process to spray it onto the base layer to form a 3 cm thick surface layer.

[0030] Example 2 (intermediate value): A method for improving the carbon fixation capacity of a food waste-mixed ecological restoration substrate in saline-alkali areas by combining arbuscular mycorrhizal fungi with decomposing bacteria comprises the following steps: Step 1: preparing a kitchen waste ecological restoration substrate carbon fixation mixture B: the kitchen waste ecological restoration substrate carbon fixation mixture B1 is mixed with arbuscular mycorrhizal fungi, kitchen waste, potato glucose agar, and corn flour agar in the ratio of 1.5:12:4.5:4.5, and the kitchen waste ecological restoration substrate carbon fixation mixture B2 is mixed with Bacillus subtilis, white rot fungi, actinomycetes, potato glucose agar, and corn flour agar in the ratio of 1:1:1:7:7, and B1 and B2 are wrapped by a polyacrylic acid outer film and separated by a curved polyacrylic acid film inside the outer film; Step 2: Constructing an ecological restoration base: Evenly mix the kitchen waste ecological restoration base material carbon fixation mixture B prepared in step 1, planting soil, soil adhesive, water retaining agent, 100-mesh corn stalks, biochar, compound fertilizer, gypsum, and bagasse powder in the proportion of 5:100:3:0.2:7:2.5:5:1.5:0.75 parts by weight, and spray onto the slope surface using a dry spraying process to form a 9 cm thick base; Step 3: preparing a kitchen waste ecological restoration substrate carbon fixation mixture A: mixing arbuscular mycorrhizal fungi, fruit and vegetable kitchen waste, bean kitchen waste, Bacillus subtilis, and Aspergillus niger fungi according to 2.5:47.5:47.5:1.5:1.5; Step 4: Construct an ecological restoration surface layer: Evenly mix the kitchen waste ecological restoration substrate carbon fixation mixture A prepared in step 3 with planting soil, soil adhesive, water retaining agent, biochar, compound fertilizer, humic acid, gypsum, bagasse powder and plant seeds in the ratio of 4:100:1.5:0.2:1.5:1:0.75:1.5:0.75 by weight, and then use a wet spraying process to spray it onto the base layer to form a 3 cm thick surface layer.

[0031] Example 3 (maximum value): A method for improving the carbon fixation capacity of a food waste-mixed ecological restoration substrate in saline-alkali areas by combining arbuscular mycorrhizal fungi with decomposing bacteria comprises the following steps: Step 1: preparing a kitchen waste ecological restoration substrate carbon fixation mixture B: the kitchen waste ecological restoration substrate carbon fixation mixture B1 is mixed with arbuscular mycorrhizal fungi, kitchen waste, potato glucose agar, and corn flour agar in the ratio of 1.5:12:4.5:4.5, and the kitchen waste ecological restoration substrate carbon fixation mixture B2 is mixed with Bacillus subtilis, white rot fungi, actinomycetes, potato glucose agar, and corn flour agar in the ratio of 1:1:1:7:7, and B1 and B2 are wrapped by a polyacrylic acid outer film and separated by a curved polyacrylic acid film inside the outer film; Step 2: Constructing an ecological restoration base: Evenly mix the kitchen waste ecological restoration base material carbon fixation mixture B prepared in step 1, planting soil, soil adhesive, water retaining agent, 100-mesh corn stalks, biochar, compound fertilizer, gypsum, and bagasse powder according to the weight ratio of 6:100:3:0.2:7:2.5:5:1.5:0.75, and spray onto the slope surface using a dry spraying process to form a 9 cm thick base; Step 3: preparing a kitchen waste ecological restoration substrate carbon fixation mixture A: mixing arbuscular mycorrhizal fungi, fruit and vegetable kitchen waste, bean kitchen waste, Bacillus subtilis, and Aspergillus niger fungi according to 2.5:47.5:47.5:1.5:1.5; Step 4: Construct an ecological restoration surface layer: Evenly mix the kitchen waste ecological restoration substrate carbon fixation mixture A prepared in step 3 with planting soil, soil adhesive, water retaining agent, biochar, compound fertilizer, humic acid, gypsum, and bagasse powder in the ratio of 6:100:1.5:0.2:1.5:1:0.75:1.5:0.75 parts by weight and plant seeds, and then use a wet spraying process to spray it onto the base layer to form a 3 cm thick surface layer.

[0032] Comparative Example 1: Compared with Example 1, in Comparative Example 1, no arbuscular mycorrhizal fungi were added to the base layer and the surface layer, and other steps and parameters were the same as those in Example 1.

[0033] Comparative Example 2: Compared with Example 1, in Comparative Example 2, no decomposing bacteria are added to the base layer and the surface layer, and other steps and parameters are the same as those in Example 1.

[0034] Comparative Example 3: Compared with Example 1, this comparative case 3 does not add kitchen waste to the base layer and the surface layer, and the other steps and parameters are the same as those of Example 1.

[0035] Control group: The control group was a vegetation ecological restoration substrate without the addition of arbuscular mycorrhizal fungi, kitchen waste and various decomposing bacteria, and the other steps and parameters were the same as those in Example 1.

[0036] For Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 2, Comparative Example 3 and the control group experimental scheme, two years after the project was implemented, the porosity, pH, conductivity, total nitrogen, total phosphorus, total potassium, total organic carbon, soluble organic carbon and microbial biomass carbon of the ecological restoration substrate were tested. See Table 1 for specific experimental data results.

[0037] Table 1 Comparison of experimental data

[0038] It can be seen from the above table that: in Example 1, Example 2 and Example 3, the method of combining arbuscular mycorrhizal fungi with decomposing bacteria to act on the ecological restoration substrate mixed with kitchen waste, the soil nitrogen, phosphorus and potassium are all higher than the comparative example and the control group, which are increased by 19.70%-28.57%, 42.5%-62.50% and 21.57%-31.66% respectively compared with the control group; the total organic carbon, soluble organic carbon and microbial biomass carbon content in the embodiment are increased by 27.51%-31.68%, 18.45%-28.14% and 22.19%-32.79% respectively compared with the control group. The above data show that the method of combining arbuscular mycorrhizal fungi with decomposing bacteria can improve the carbon fixation capacity of the ecological restoration substrate mixed with kitchen waste in saline-alkali areas and improve the effect of ecological restoration.

[0039] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. A substrate for improving the carbon fixation capacity of an ecological restoration substrate containing kitchen waste by using arbuscular mycorrhizae, characterized in that: It includes an ecological restoration substrate surface layer and an ecological restoration substrate base layer; The surface layer of the ecological restoration substrate is prepared by uniformly mixing the kitchen waste ecological restoration substrate carbon fixation mixture A, planting soil, soil adhesive, water retaining agent, biochar, compound fertilizer, humic acid, gypsum, bagasse powder and plant seeds in a certain weight ratio; The kitchen waste ecological restoration substrate carbon fixation mixture A is prepared by mixing arbuscular mycorrhizal fungi, fruit and vegetable kitchen waste, bean kitchen waste, Bacillus subtilis, and Aspergillus niger fungi according to a certain weight ratio; The ecological restoration substrate base is prepared by uniformly mixing kitchen waste ecological restoration substrate carbon fixation mixture B, planting soil, soil adhesive, water retaining agent, 100-mesh corn stalks, biochar, compound fertilizer, gypsum, and bagasse powder according to a certain weight ratio; The kitchen waste ecological restoration substrate carbon fixation mixture B is composed of kitchen waste ecological restoration substrate carbon fixation mixture B1 and B2, B1 and B2 are wrapped by a polyacrylic acid outer film and separated by a curved polyacrylic acid film inside the outer film; the kitchen waste ecological restoration substrate carbon fixation mixture B1 is prepared by mixing arbuscular mycorrhizal fungi, kitchen waste, potato dextrose agar, and corn flour agar in a certain weight ratio; the kitchen waste ecological restoration substrate carbon fixation mixture B2 is prepared by mixing Bacillus subtilis, white rot fungi, actinomycetes, potato dextrose agar, and corn flour agar in a certain weight ratio.

2. According to claim 1, a substrate for improving the carbon fixation capacity of an ecological restoration substrate containing kitchen waste by using arbuscular mycorrhizae, characterized in that: The weight ratio of the kitchen waste ecological restoration substrate carbon fixation mixture A, planting soil, soil adhesive, water retaining agent, biochar, compound fertilizer, humic acid, gypsum and bagasse powder in the surface layer of the ecological restoration substrate is 2-6:100:1-2:0.1-0.3:1-2:0.8-1.2:0.5-1:1-2:0.5-1.

3. According to claim 1, a substrate for improving the carbon fixation capacity of an ecological restoration substrate containing kitchen waste by using arbuscular mycorrhizae, characterized in that: The weight ratio of arbuscular mycorrhizal fungi, fruit and vegetable kitchen waste, bean kitchen waste, Bacillus subtilis and Aspergillus niger in the kitchen waste ecological restoration substrate carbon fixation mixture A is 2-3:45-50:45-50:1-2:1-2.

4. A substrate for improving carbon fixation capacity of ecological restoration substrate containing kitchen waste by using arbuscular mycorrhizae according to claim 1, characterized in that: The weight ratio of the kitchen waste ecological restoration substrate carbon fixation mixture B, planting soil, soil adhesive, water retaining agent, corn stalks with a mesh size of 100, biochar, compound fertilizer, gypsum, and bagasse powder in the ecological restoration substrate base layer is 4-6:100:2-4:0.1-0.3:6-8:2-3:4-6:1-2:0.5-1.

5. The substrate for improving carbon fixation capacity of ecological restoration substrate containing kitchen waste by using arbuscular mycorrhizae according to claim 1, characterized in that: The weight ratio of arbuscular mycorrhizal fungi agent, kitchen waste, potato glucose agar, and corn flour agar in the kitchen waste ecological restoration substrate carbon fixation mixture B1 is 1-2:10-14:4-5:4-5; The weight ratio of Bacillus subtilis, white rot fungi, actinomycetes, potato glucose agar and corn flour agar in the kitchen waste ecological restoration substrate carbon fixation mixture B2 is 1:1:1:6-8:6-8.

6. The substrate for improving carbon fixation capacity of ecological restoration substrate containing kitchen waste by using arbuscular mycorrhizae according to claim 1, characterized in that: The arbuscular mycorrhizal fungi are a mixture of Glomus mosseae and Glomus intraradices in a ratio of 1:

1.

7. The substrate for improving carbon fixation capacity of ecological restoration substrate containing kitchen waste by using arbuscular mycorrhizae according to claim 1, characterized in that: The planting soil is taken from the topsoil of 0-10 cm in the saline-alkali area, the soil is air-dried and crushed, and passed through a 10 mm sieve; The soil adhesive is a mixture of cement, bentonite and gelatin in a ratio of 1:1:1; The water retaining agent is a mixture of polyvinyl amide and polymer hydrogel in a ratio of 1:1; The biochar is prepared by mixing mineral biochar and wood biochar that have passed a 200-mesh sieve in a ratio of 1:

1.

8. The substrate for improving carbon fixation capacity of ecological restoration substrate containing kitchen waste by using arbuscular mycorrhizae according to claim 1, characterized in that: The compound fertilizer is composed of quick-acting fertilizer and slow-release fertilizer, wherein the quick-acting fertilizer N, P, and K are mixed in a ratio of ammonium sulfate: diammonium phosphate: potassium sulfate of 1-2:1-2:1-2, and the slow-release fertilizer is polyamino acid slow-release fertilizer.

9. The substrate for improving carbon fixation capacity of ecological restoration substrate containing kitchen waste by using arbuscular mycorrhizae according to claim 1, characterized in that: The plant seed density is 10-30 grams per square meter, and the plant seeds are mixed with bermudagrass, alfalfa, ryegrass, magnolia, raspberry, and poplar in a ratio of 3:3:3:2:2:1; The kitchen waste is dried in advance and then crushed through a 1 mm sieve. The kitchen waste in the kitchen waste ecological restoration base material carbon fixation mixture B1 is also fermented.

10. A method for improving the carbon fixation capacity of a substrate for ecological restoration containing kitchen waste in saline-alkali areas by using the substrate for improving the carbon fixation capacity of a substrate for ecological restoration containing kitchen waste using arbuscular mycorrhizae as described in any one of claims 1 to 9, characterized in that: The following steps are involved: Step 1: preparing a kitchen waste ecological restoration substrate carbon fixation mixture B: mixing the treated kitchen waste, potato dextrose agar, corn flour agar and arbuscular mycorrhizal fungi in a set weight ratio to prepare B1, and mixing Bacillus subtilis, white rot fungi, actinomycetes, potato dextrose agar and corn flour agar in the above weight ratio to prepare B2, B1 and B2 are wrapped by a polyacrylic acid outer membrane and separated by a curved polyacrylic acid membrane inside the outer membrane; Step 2: Constructing an ecological restoration base: Evenly mix the kitchen waste ecological restoration base material carbon fixation mixture B prepared in step 1, planting soil, soil adhesive, 100-mesh corn stalks, biochar, and compound fertilizer according to the above weight parts, and use a dry spraying process to spray on the slope surface to form a base with a thickness of 8-10 cm; Step 3: preparing a kitchen waste ecological restoration substrate carbon fixation mixture A: mixing arbuscular mycorrhizal fungi, fruit and vegetable kitchen waste, bean kitchen waste, Bacillus subtilis, and Aspergillus niger fungi according to a set weight ratio; Step 4: Construct the ecological restoration surface layer: Mix the kitchen waste ecological restoration substrate carbon fixation mixture A prepared in step 3 with planting soil, soil adhesive, water retaining agent, biochar, compound fertilizer and plant seeds in a set weight ratio, and use the wet spraying process to spray it on the base to form a surface layer with a thickness of 2-3 cm.

Images