Preparation method of trichoderma bio-organic fertilizer for antagonizing pineapple heart rot

Abstract

The invention discloses a preparation method of a trichoderma bio-organic fertilizer for antagonizing pineapple heart rot, which comprises the following steps: preparing crop straws and an organic fertilizer, inoculating trichoderma strains into a liquid culture medium, carrying out liquid submerged fermentation, inoculating hyphae and spores obtained by liquid fermentation into a solid culture medium, and carrying out solid fermentation to obtain the trichoderma bio-organic fertilizer for antagonizing pineapple heart rot. Straw, sugar refinery filter mud and an organic fertilizer are used as matrixes, trichoderma hypha and spores obtained through solid fermentation are mixed with a common organic fertilizer according to a certain proportion, the mixed material is subjected to forming treatment, the granular trichoderma bio-organic fertilizer is prepared, and the organic fertilizer is livestock and poultry manure and agricultural waste. The trichoderma bio-organic fertilizer prepared by the preparation method of the pineapple heart rot resistant trichoderma bio-organic fertilizer not only has good nutrient durability, but also has a very good inhibition effect on pineapple heart rot resistance of plants, so that the use amount of the organic fertilizer is greatly reduced, and the production cost is reduced. Meanwhile, the fertilizer also has a very good disease-resistant effect on plants.

Description

Technical Field

[0001] The invention relates to the technical field of preparation of Trichoderma bio-organic fertilizer for antagonizing pineapple heart rot, in particular to a preparation method of Trichoderma bio-organic fertilizer for antagonizing pineapple heart rot. Background Art

[0002] Trichoderma belongs to the phylum Ascomycota, class Sclerotium, orders Hypocreales, family Hypocreaceae, and genus Trichoderma. It is widely found in soil under different environmental conditions. Most Trichoderma can produce a variety of biologically active substances that are antagonistic to plant pathogenic fungi, bacteria and insects, such as cell wall degrading enzymes and secondary metabolites, and can improve the stress resistance of crops, promote plant growth and increase agricultural product yields. Therefore, it is widely used in biological control, biological fertilizers and soil conditioners. Due to the serious negative impact of chemical pesticides on the environment, the environmentally friendly biological pesticide Trichoderma has received widespread attention.

[0003] Trichoderma is one of the most widely used biofungicides in agricultural production. It can inhibit up to 29 plant fungal pathogens, including Phytophthora, Pythium, Fusarium, Botrytis, Rhizoctonia, etc., by parasitism, antibacterial (producing antibacterial substances and antibiotics), competition for nutrients and space, and inducing systemic resistance of plants. Some common biocontrol Trichoderma mainly include Trichoderma harzianum, Trichoderma acanthosporum and Trichoderma viride. During the production process of Trichoderma, a large amount of Trichoderma fermentation residue is produced, which is easy to pollute water sources and air if discharged or incinerated directly. Since Trichoderma residue contains rich nutrients, a large number of active Trichoderma biocontrol bacteria and low cost, Trichoderma residue is often mixed with livestock manure as organic fertilizer and applied directly to the field without fermentation. However, Trichoderma residue itself contains a large number of antibiotic resistance genes (ARGs), and nutrients are not fully decomposed and released when directly mixed and applied.

[0004] However, many fertilizers currently do not contain Trichoderma, and some organic fertilizers containing Trichoderma are not durable enough in terms of nutrient endurance, and the inhibitory effect on plants against pineapple heart rot is not good enough. Therefore, an improved technology is urgently needed to solve this problem existing in the prior art. Summary of the invention

[0005] The purpose of the present invention is to provide a method for preparing a Trichoderma bio-organic fertilizer that not only has good nutrient durability of the fertilizer, but also has a good inhibitory effect on the resistance of plants to pineapple heart rot, greatly reducing the usage of organic fertilizers, and at the same time can have a good disease resistance effect on plants and antagonize pineapple heart rot, so as to solve the problems raised in the above-mentioned background technology.

[0006] To achieve the above object, the present invention provides the following technical scheme: a method for preparing a Trichoderma bio-organic fertilizer for antagonizing pineapple heart rot, the method for preparing the Trichoderma bio-organic fertilizer for antagonizing pineapple heart rot comprising:

[0007] Raw material preparation: prepare suitable raw materials, crop straw and organic fertilizer;

[0008] Liquid fermentation: inoculate the Trichoderma strain into liquid culture medium for submerged fermentation;

[0009] Solid fermentation: The mycelium and spores obtained from liquid fermentation are inoculated onto solid culture medium, using straw, sugar mill filter mud, and organic fertilizer as substrates;

[0010] Material mixing: Mix the Trichoderma mycelium and spores obtained by solid fermentation with ordinary organic fertilizer in a certain proportion;

[0011] Molding treatment: The mixed materials are molded to make granular Trichoderma bio-organic fertilizer.

[0012] Preferably, the crop straw is one or more of wheat straw, rice straw and corn straw;

[0013] The organic fertilizer is livestock and poultry manure and agricultural waste. The livestock and poultry manure and agricultural waste are sterilized by ultraviolet rays. During the ultraviolet sterilization process, the livestock and poultry manure and agricultural waste are turned over every 5 minutes.

[0014] Preferably, during the liquid fermentation process, the fermentation temperature is controlled at 25-30°C, the pH value is 3.5-5.8, and the humidity is maintained at 90-95%;

[0015] The liquid fermentation cycle is 110 hours, and the spore production is 10^8 thick-walled spores / ml.

[0016] Preferably, during the solid fermentation process, the crop straws are neatly laid out and then water is sprinkled on the surface, a layer of organic fertilizer is evenly spread on the wet crop straws, and another layer of crop straw is spread on the surface of the organic fertilizers;

[0017] Good ventilation conditions must be maintained during the solid fermentation process to ensure the growth of mycelium and spores.

[0018] Preferably, the substrate needs to be adjusted to a water content of 60%-65% and then fermented at 22°C-28°C for 7 days.

[0019] Preferably, the material mixing ratio is 1:9.

[0020] Preferably, the material is crushed during the molding process, and the crushed material is mixed with Trichoderma hyphae and spores for processing;

[0021] The evenly mixed organic fertilizer is dried and then sealed in bags.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] (1) Cover the outside of the pile with a material film, and poke holes in the plastic film used for covering to ensure that the bacteria inside the fertilizer pile have sufficient oxygen during the fermentation process. This not only enables the pile to ferment better in a warm environment, but also ensures that there is sufficient oxygen. Good ventilation conditions must be maintained during solid fermentation to ensure the growth of mycelium and spores;

[0024] (2) The Trichoderma bio-organic fertilizer prepared by the method for preparing the Trichoderma bio-organic fertilizer resistant to pineapple heart rot not only has good nutrient durability of the fertilizer, but also has a good inhibitory effect on the resistance of plants to pineapple heart rot, greatly reducing the amount of organic fertilizer used, and at the same time can also have a good disease resistance effect on plants. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 The present invention is a flow chart of the preparation method of the Trichoderma bio-organic fertilizer for antagonizing pineapple heart rot. DETAILED DESCRIPTION

[0026] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] See also Figure 1 The present invention provides a technical solution: a method for preparing a Trichoderma bio-organic fertilizer for antagonizing pineapple heart rot, the method for preparing the Trichoderma bio-organic fertilizer for antagonizing pineapple heart rot comprising:

[0028] Raw material preparation: prepare suitable raw materials, crop straw and organic fertilizer;

[0029] Liquid fermentation: inoculate the Trichoderma strain into liquid culture medium for submerged fermentation;

[0030] Solid fermentation: The mycelium and spores obtained from liquid fermentation are inoculated onto solid culture medium, using straw, sugar mill filter mud, and organic fertilizer as substrates;

[0031] Material mixing: Mix the Trichoderma mycelium and spores obtained by solid fermentation with ordinary organic fertilizer in a certain proportion;

[0032] Molding treatment: The mixed materials are molded to make granular Trichoderma bio-organic fertilizer.

[0033] Prepare suitable raw materials, crop straw and organic fertilizer, the crop straw is one or more of wheat straw, rice straw, corn straw, the organic fertilizer is livestock and poultry manure, agricultural waste, the livestock and poultry manure, and agricultural waste are sterilized by ultraviolet light, and during the ultraviolet light sterilization process, the livestock and poultry manure and agricultural waste are turned over every 5 minutes, and turned over continuously for 4-5 times.

[0034] The Trichoderma strain is inoculated into a liquid culture medium for liquid deep fermentation. During the liquid fermentation process, the fermentation temperature is controlled at 25-30°C, the pH value is 3.5-5.8, the humidity is maintained at 90-95%, the liquid fermentation cycle is 110 hours, and the spore production is 10^8 thick-walled spores / ml.

[0035] During the solid fermentation process, the crop straw is neatly laid out and then water is sprinkled on the surface to break up the organic fertilizer. The organic fertilizer is evenly spread on the wet crop straw, and then another layer of crop straw is spread on the surface of the organic fertilizer. The above process is repeated to form a stack of crop straw and organic fertilizer. After the straw and organic fertilizer are laid, the outside of the pile is covered with a material film, and holes are poked in the plastic film used for covering to ensure that the bacteria inside the fertilizer pile have enough oxygen during the fermentation process. This not only allows the pile to ferment better in a heat-insulating environment, but also ensures that there is enough oxygen. Good ventilation conditions must be maintained during the solid fermentation process to ensure the growth of mycelium and spores.

[0036] The matrix is ​​mixed with straw, sugar factory filter mud and organic fertilizer in a ratio of 1:1:3. The mixed matrix is ​​adjusted to a moisture content of 60%-65%, and then fermented at 22°C-28°C for 7 days. During the material mixing process, the fermented mixed matrix is ​​mixed with Trichoderma hyphae and spores in a ratio of 1:9.

[0037] The mixed materials are subjected to molding treatment. During the molding process, the materials are first crushed and then mixed with Trichoderma hyphae and spores. The crushed mixed organic materials are compressed and supported by a molding pelletizer to form small cylindrical pellets. The molded Trichoderma bio-organic fertilizer is dried in the sun, and the molded organic fertilizer is sealed in bags after drying.

[0038] Embodiment 1,

[0039] Prepare suitable raw materials, crop straw and organic fertilizer, the crop straw is one or more of wheat straw, rice straw, corn straw, the organic fertilizer is livestock and poultry manure, agricultural waste, the livestock and poultry manure, and agricultural waste are sterilized by ultraviolet light, and during the ultraviolet light sterilization process, the livestock and poultry manure and agricultural waste are turned over every 5 minutes, and turned over continuously for 4-5 times.

[0040] The Trichoderma strain was inoculated into a liquid culture medium for liquid deep fermentation. During the liquid fermentation, the fermentation temperature was controlled at 25°C, the pH value was 3.6, the humidity was maintained at 93%, the liquid fermentation cycle was 110 hours, and the spore production was 10^8 thick-walled spores / ml.

[0041] The matrix is ​​mixed with straw, sugar factory filter mud and organic fertilizer in a ratio of 1:1:3. The mixed matrix is ​​adjusted to a moisture content of 65% and then fermented at 23°C for 7 days. During the material mixing process, the fermented mixed matrix is ​​mixed with Trichoderma hyphae and spores in a ratio of 1:9.

[0042] Embodiment 2,

[0043] Prepare suitable raw materials, crop straw and organic fertilizer, the crop straw is one or more of wheat straw, rice straw, corn straw, the organic fertilizer is livestock and poultry manure, agricultural waste, the livestock and poultry manure, and agricultural waste are sterilized by ultraviolet light, during the ultraviolet light sterilization process, the livestock and poultry manure and agricultural waste are turned over every 5 minutes, and turned over continuously for 4-5 times, so that the bacteria in the organic fertilizer are completely eliminated.

[0044] The Trichoderma strain was inoculated into a liquid culture medium for deep liquid fermentation. During the liquid fermentation, the fermentation temperature was controlled at 26°C, the pH value was 3.9, the humidity was maintained at 93%, the liquid fermentation cycle was 110 hours, and the spore production was 10^8 thick-walled spores / ml.

[0045] The matrix is ​​mixed with straw, sugar factory filter mud and organic fertilizer in a ratio of 1:1:3. The mixed matrix is ​​adjusted to a moisture content of 65% and then fermented at 23°C for 7 days. During the material mixing process, the fermented mixed matrix is ​​mixed with Trichoderma hyphae and spores in a ratio of 1:9.

[0046] Embodiment 3,

[0047] Prepare suitable raw materials, crop straw and organic fertilizer, the crop straw is one or more of wheat straw, rice straw, corn straw, the organic fertilizer is livestock and poultry manure, agricultural waste, the livestock and poultry manure, and agricultural waste are sterilized by ultraviolet light, and during the ultraviolet light sterilization process, the livestock and poultry manure and agricultural waste are turned over every 5 minutes, and turned over continuously for 4-5 times.

[0048] The Trichoderma strain was inoculated into a liquid culture medium for deep liquid fermentation. During the liquid fermentation, the fermentation temperature was controlled at 27°C, the pH value was 4.5, the humidity was maintained at 93%, the liquid fermentation cycle was 110 hours, and the spore production was 10^8 thick-walled spores / ml.

[0049] The matrix is ​​mixed with straw, sugar factory filter mud and organic fertilizer in a ratio of 1:1:3. The mixed matrix is ​​adjusted to a moisture content of 65% and then fermented at 23°C for 7 days. During the material mixing process, the fermented mixed matrix is ​​mixed with Trichoderma hyphae and spores in a ratio of 1:9.

[0050] Embodiment 4,

[0051] Prepare suitable raw materials, crop straw and organic fertilizer, the crop straw is one or more of wheat straw, rice straw, corn straw, the organic fertilizer is livestock and poultry manure, agricultural waste, the livestock and poultry manure, and agricultural waste are sterilized by ultraviolet light, and during the ultraviolet light sterilization process, the livestock and poultry manure and agricultural waste are turned over every 5 minutes, and turned over continuously for 4-5 times.

[0052] The Trichoderma strain was inoculated into a liquid culture medium for liquid deep fermentation. During the liquid fermentation, the fermentation temperature was controlled at 28°C, the pH value was 5.0, the humidity was maintained at 93%, the liquid fermentation cycle was 110 hours, and the spore production was 10^8 thick-walled spores / ml.

[0053] The matrix is ​​mixed with straw, sugar factory filter mud and organic fertilizer in a ratio of 1:1:3. The mixed matrix is ​​adjusted to a moisture content of 65% and then fermented at 23°C for 7 days. During the material mixing process, the fermented mixed matrix is ​​mixed with Trichoderma hyphae and spores in a ratio of 1:9.

[0054] Embodiment 5,

[0055] Prepare suitable raw materials, crop straw and organic fertilizer, the crop straw is one or more of wheat straw, rice straw, corn straw, the organic fertilizer is livestock and poultry manure, agricultural waste, the livestock and poultry manure, and agricultural waste are sterilized by ultraviolet light, and during the ultraviolet light sterilization process, the livestock and poultry manure and agricultural waste are turned over every 5 minutes, and turned over continuously for 4-5 times.

[0056] The Trichoderma strain was inoculated into a liquid culture medium for liquid deep fermentation. During the liquid fermentation, the fermentation temperature was controlled at 28°C, the pH value was 5.5, the humidity was maintained at 95%, the liquid fermentation cycle was 110 hours, and the spore production was 10^8 thick-walled spores / ml.

[0057] The matrix is ​​mixed with straw, sugar factory filter mud and organic fertilizer in a ratio of 1:1:3. The mixed matrix is ​​adjusted to a moisture content of 65% and then fermented at 23°C for 7 days. During the material mixing process, the fermented mixed matrix is ​​mixed with Trichoderma hyphae and spores in a ratio of 1:9.

[0058] The Trichoderma bio-organic fertilizers prepared under different preparation conditions were compared. During the liquid fermentation process, the fermentation temperature was controlled at 27°C, the pH value was 4.5, the humidity was maintained at 93%, the liquid fermentation cycle was 110 hours, the spore production was 10^8 thick-walled spores / ml, the matrix was mixed with straw, sugar factory filter mud, and organic fertilizer in a ratio of 1:1:3, the mixed matrix was adjusted to a water content of 65%, and then fermented at 23°C for 7 days. During the material mixing process, the fermented mixed matrix was mixed with Trichoderma hyphae and spores in a ratio of 1:9. The Trichoderma bio-organic fertilizer had the best comprehensive performance in fertilizer nutrients and antagonism to pineapple heart rot. Therefore, the preparation conditions were used to prepare the Trichoderma bio-organic fertilizer.

[0059] The Trichoderma bio-organic fertilizer prepared by the method for preparing the pineapple heart rot resistant Trichoderma bio-organic fertilizer not only has good nutrient durability of the fertilizer, but also has a good inhibitory effect on the resistance of plants to pineapple heart rot, greatly reduces the use amount of organic fertilizer, and can also have a good disease resistance effect on plants.

[0060] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for preparing a Trichoderma bio-organic fertilizer for antagonizing pineapple heart rot, characterized in that: The preparation method of the Trichoderma bio-organic fertilizer for antagonizing pineapple heart rot comprises: Raw material preparation: prepare suitable raw materials, crop straw and organic fertilizer; Liquid fermentation: inoculate the Trichoderma strain into liquid culture medium for submerged fermentation; Solid fermentation: The mycelium and spores obtained from liquid fermentation are inoculated onto solid culture medium, using straw, sugar mill filter mud, and organic fertilizer as substrates; Material mixing: Mix the Trichoderma mycelium and spores obtained by solid fermentation with ordinary organic fertilizer in a certain proportion; Molding treatment: The mixed materials are molded to make granular Trichoderma bio-organic fertilizer.

2. the preparation method of a kind of Trichoderma bio-organic fertilizer of antagonism pineapple heart rot according to claim 1, is characterized in that: The crop straw is one or more of wheat straw, rice straw and corn straw; The organic fertilizer is livestock and poultry manure and agricultural waste. The livestock and poultry manure and agricultural waste are sterilized by ultraviolet rays. During the ultraviolet sterilization process, the livestock and poultry manure and agricultural waste are turned over every 5 minutes.

3. the preparation method of the Trichoderma bio-organic fertilizer of a kind of antagonism pineapple heart rot according to claim 1, is characterized in that: During the liquid fermentation process, the fermentation temperature is controlled at 25-30°C, the pH value is 3.5-5.8, and the humidity is maintained at 90-95%; The liquid fermentation cycle is 110 hours, and the spore production is 10^8 thick-walled spores / ml.

4. the preparation method of a kind of Trichoderma bio-organic fertilizer of antagonism pineapple heart rot according to claim 1, is characterized in that: During the solid fermentation process, the crop straws are neatly laid out and then water is sprinkled on the surface, a layer of organic fertilizer is evenly spread on the wet crop straws, and another layer of crop straw is spread on the surface of the organic fertilizers; Good ventilation conditions must be maintained during the solid fermentation process to ensure the growth of mycelium and spores.

5. the preparation method of a kind of Trichoderma bio-organic fertilizer of antagonism pineapple heart rot according to claim 1, is characterized in that: The substrate needs to be adjusted to a moisture content of 60%-65%, and then fermented at 22°C-28°C for 7 days.

6. the preparation method of a kind of Trichoderma bio-organic fertilizer of antagonism pineapple heart rot according to claim 1, is characterized in that: The material mixing ratio is 1:

9.

7. The preparation method of a Trichoderma bio-organic fertilizer for antagonizing pineapple heart rot according to claim 1, characterized in that: In the molding process, the material is crushed, and the crushed material is mixed with Trichoderma hyphae and spores for processing; The evenly mixed organic fertilizer is dried and then sealed in bags.

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