Microbial agent composition for promoting nitrogen absorption of plants and preparation process of microbial agent composition

Abstract

The invention relates to the technical field of microbial agents, in particular to a microbial agent composition for promoting nitrogen absorption of plants and a preparation process of the microbial agent composition. The preparation process comprises the following steps: preparing cymbidium ensifolium root system soil compound bacteria liquid; preparing a treated fiber membrane; preparing a composite organic silanol solution; and preparing the microbial agent composition for promoting nitrogen absorption of plants. Cymbidium ensifolium root system soil is cultured to prepare cymbidium ensifolium root system soil compound bacteria liquid, the cymbidium ensifolium root system soil compound bacteria liquid, a glucosamine solution and a silanol solution are jointly used for preparing the compound aerogel, the glucosamine and the silanol provide a framework and a grid structure, the cymbidium ensifolium root system soil compound bacteria liquid is attached to the framework and the grid structure, and the composite aerogel is prepared. The composite aerogel structure has a better microorganism immobilizing effect compared with a solution, and the cymbidium ensifolium root system soil composite bacterial liquid secretes plant hormones to stimulate root system cell development, so that the nitrogen absorption capacity of plants is enhanced.

Description

Technical Field

[0001] The invention relates to the technical field of microbial agents, and in particular to a microbial agent composition for promoting plant nitrogen absorption and a preparation process thereof. Background Art

[0002] Nitrogen is one of the key nutrients required for plant growth and is involved in the synthesis of various biological molecules such as proteins, enzymes, and chlorophyll. Adequate nitrogen supply is crucial to crop yield and quality. However, most of the nitrogen in nature exists in the atmosphere in the form of nitrogen gas, which cannot be directly used by plants. Although traditional inorganic nitrogen fertilizers such as urea and ammonium nitrate can quickly provide a large amount of nitrogen, they also have obvious limitations. For example, large-scale application may cause nitrogen loss into water bodies or the atmosphere, causing environmental pollution; long-term use may cause soil compaction, reducing soil fertility and biodiversity.

[0003] Therefore, people often choose to use microbial agents to promote plant nitrogen absorption. The ways to promote nitrogen absorption include converting nitrogen in the atmosphere into ammonia nitrogen for plant absorption; secreting plant hormones, dissolving phosphorus and potassium, etc., indirectly promoting plant growth and nitrogen absorption. Spraying such microbial agents requires that microorganisms and plants can coexist for a long time to achieve the effect. However, the existing microbial agent spraying agents are easily lost due to factors such as rain and evaporation, resulting in the inability of microbial agents to act on plants for a long time. The effect of promoting plant nitrogen absorption is limited, and it is not as good as organic fertilizers. Therefore, the present invention provides a microbial agent composition for promoting plant nitrogen absorption and a preparation process thereof to solve the above problems. Summary of the invention

[0004] In view of the shortcomings of the prior art, the object of the present invention is to provide a microbial agent composition for promoting plant nitrogen absorption and a preparation process thereof.

[0005] S1: Preparation of soil compound bacterial solution for the root system of Cymbidium orchid S1.1: Take a well-growing Jianlan plant, put the Jianlan root and 3mm soil around the root into a centrifuge tube, shake and wash the precipitate at 0-4℃ to obtain a soil suspension; S1.2: Add 5-8g / L trehalose to the basal culture medium, then add the soil suspension to the basal culture medium and culture at 28°C for 8-10 days. Pick out the formed strains and transfer them to LB culture medium plates, culture at 37°C for 2 days to obtain the composite bacterial solution of the root soil of Jianlan.

[0006] S2: Preparation and treatment of fiber membrane S2.1: dissolving 0.5-1 parts by weight of polyester particles in 4.5-5 parts by weight of hexafluoroisopropanol, stirring magnetically for 24-25 hours to obtain a polyester spinning solution, and spinning with an electrospinning device to prepare a polyester nanofiber membrane; S2.2: The obtained polyester nanofiber membrane is treated with a vacuum plasma treatment apparatus at a power of 180 W for 20-25 s to obtain a treated fiber membrane.

[0007] Furthermore, the voltage of the electrospinning device is 17 kV, the receiving distance is 15 cm, the injection speed is 0.1 mm / min, and the rotation speed is 80 r / min.

[0008] S3: Preparation of composite organosilanol solution S3.1: adding 0.2-0.4 parts by mass of polyglucosamine to 20-25 parts by volume of 0.2% acetic acid solution, and stirring with a magnetic force of 500-550 r / min at 50-60° C. for 30-35 min to obtain a polyglucosamine solution; S3.2: Add 0.2-0.3 parts by mass of the composite bacterial solution from the root soil of Cymbidium orchid into 300-320 parts by volume of ultrapure water, and stir magnetically at 500-550 r / min for 10-15 min to obtain a diluted bacterial solution; S3.3: Mix tetraethyl orthosilicate, ethanol and water in a volume ratio of 1:2:(4-5), adjust the pH to 2-3 with 1-1.5 mol / L hydrochloric acid, and stir continuously under magnetic stirring at room temperature for 24-25 h to obtain a silanol solution; S3.4: The polyglucosamine solution and the diluted bacterial solution were mixed in a volume ratio of 2:(4-5), heated to 40-45°C, and magnetically stirred for 24 hours to obtain a mixed solution. The mixed solution was centrifuged at a speed of 8000-8500 r / min for 5-10 minutes, and then the supernatant was removed, and the mixture was washed with ultrapure water for 3 times. Then, a silanol solution with an equal volume to the polyglucosamine solution was added to obtain a composite organic silanol solution; S3.5: Immerse the treated fiber membrane in a composite organosilicone solution, adjust the pH to 6-6.5 with 0.5 mol / L ammonia water, perform polycondensation for 6-8 hours to obtain a wet gel, age it at 40-45°C for 48-50 hours, and then place it in the composite treatment solution for modification for 12-14 hours. Then add 20-25% of the total mass of n-hexane in the system, react at 24-26°C for 6-8 hours, and then let it stand for 24-26 hours to obtain a composite aerogel.

[0009] Furthermore, the composite treatment liquid is prepared by mixing trimethylchlorosilane, ethanol and n-hexane in a ratio of 2:1:8.

[0010] S4: Preparation of a microbial agent composition for promoting plant nitrogen absorption The composite bacterial liquid in the soil of the root system of Cymbidium orchid was diluted with 10-15 times of deionized water, and then the composite aerogel was added, with a solid-liquid ratio of 1g: (25-30)mL, and then 25-35% of the total mass of the system of humic acid was added to obtain a microbial agent composition that promotes plant nitrogen absorption.

[0011] A microbial agent composition for promoting plant nitrogen absorption is prepared by the above-mentioned preparation process of the microbial agent composition for promoting plant nitrogen absorption.

[0012] Compared with the prior art, the present invention has at least the following beneficial effects: 1. The present invention takes the root soil of Jianlan for cultivation to prepare the composite bacterial liquid of the root soil of Jianlan, and prepares the composite bacterial liquid of the root soil of Jianlan together with the polyglucosamine solution and the silanol solution to prepare the composite aerogel. The polyglucosamine and silanol provide a skeleton and a grid structure, so that the composite bacterial liquid of the root soil of Jianlan adheres thereto, thereby forming a highly porous composite aerogel structure. The composite aerogel structure has a better effect of fixing microorganisms than the solution, and the root microorganisms can decompose organic matter and release nitrogen, phosphorus, potassium and other essential nutrients for plants. After these elements are fixed in the aerogel, they can be slowly released to continuously provide nutrients for the plants. The aerogel structure has excellent water absorption and water retention capacity, which can effectively reduce water loss. At the same time, it can also fix nutrients to prevent the nutrients and microorganisms contained in the microbial agent composition from being lost with rainwater or irrigation water after spraying. Therefore, by fixing microorganisms and nutrients through aerogels, it can also reduce dependence on chemical fertilizers and reduce the risk of environmental pollution. The composite bacterial liquid of the root soil of Jianlan secretes plant hormones to stimulate the development of root cells and generate a more developed root system, thereby enhancing the ability of plants to absorb nitrogen.

[0013] 2. The present invention forms a composite aerogel by coating a treated fiber membrane prepared from polyester particles on the outer layer of a wet gel. The polyester fiber membrane has good wear resistance and air permeability, can prolong the storage time of the microbial agent composition, and the polyester fiber membrane provides a stable habitat for root microorganisms, helps to form a healthy rhizosphere microecology, promotes the synergistic effect between plant roots and beneficial microorganisms, and thus improves the plant's ability to absorb nitrogen. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] The accompanying drawings, which are incorporated herein and constitute a part of the specification, illustrate embodiments of the present disclosure and, together with the description, further serve to explain the principles of the present disclosure and to enable those skilled in the relevant art to make and use the present disclosure.

[0015] Figure 1 This is a flow chart of the preparation process of a microbial agent composition for promoting plant nitrogen absorption used in an embodiment of the present invention. DETAILED DESCRIPTION

[0016] The following is a detailed description of a microbial agent composition for promoting plant nitrogen absorption and its preparation process provided by the present invention in conjunction with the accompanying drawings and specific embodiments. At the same time, it is explained here that in order to make the embodiments more detailed, the following embodiments are the best and preferred embodiments, and those skilled in the art may also adopt other alternatives to implement some known technologies; and the accompanying drawings are only for a more specific description of the embodiments, and are not intended to specifically limit the present invention.

[0017] Example 1: A preparation process of a microbial agent composition for promoting nitrogen absorption in plants, such as Figure 1 As shown, the following steps are included: S1: Preparation of soil compound bacterial solution for the root system of Cymbidium orchid S1.1: Take a well-growing Jianlan plant, put the Jianlan root and 3mm soil around the root into a centrifuge tube, shake and wash the precipitate at 0℃ to obtain a soil suspension.

[0018] S1.2: Add 5 g / L trehalose to the basal culture medium, then add the soil suspension to the basal culture medium and culture at 28°C for 8 days. Pick out the formed strains and transfer them to LB culture medium plates, culture at 37°C for 2 days to obtain the composite bacterial solution of the root soil of Jianlan.

[0019] S2: Preparation and treatment of fiber membrane S2.1: 0.5 parts by mass of polyester particles were dissolved in 4.5 parts by mass of hexafluoroisopropanol, and the polyester spinning solution was obtained by magnetic stirring for 24 hours. The polyester nanofiber membrane was prepared by spinning using an electrospinning device. The voltage of the electrospinning device was 17 kV, the receiving distance was 15 cm, the injection speed was 0.1 mm / min, and the rotation speed was 80 r / min. S2.2: The obtained polyester nanofiber membrane is treated with a vacuum plasma treatment apparatus at a power of 180 W for 20 seconds to obtain a treated fiber membrane.

[0020] S3: Preparation of composite organosilanol solution S3.1: adding 0.2 parts by mass of polyglucosamine to 20 parts by volume of 0.2% acetic acid solution, and stirring with magnetic force at 500 rpm for 30 min at 50°C to obtain a polyglucosamine solution; S3.2: Add 0.2 parts by mass of the composite bacterial solution from the root soil of Cymbidium orchid to 300 parts by volume of ultrapure water, and stir magnetically at 500 rpm for 10 minutes to obtain a diluted bacterial solution; S3.3: Tetraethyl orthosilicate, ethanol and water were mixed in a volume ratio of 1:2:4, and the pH value was adjusted to 2 with 1 mol / L hydrochloric acid. The mixture was continuously stirred with magnetic stirring at room temperature for 24 h to obtain a silanol solution; S3.4: The polyglucosamine solution and the diluted bacterial solution were mixed in a volume ratio of 2:4, heated to 40°C, and magnetically stirred for 24 hours to obtain a mixed solution. The mixed solution was centrifuged at a speed of 8000 r / min for 5 minutes, and then the supernatant was removed, and the mixture was washed with ultrapure water for 3 times. Then, a silanol solution with a volume equal to that of the polyglucosamine solution was added to obtain a composite organic silanol solution; S3.5: The treated fiber membrane is immersed in a composite organosilanol solution, the pH is adjusted to 6 with 0.5 mol / L ammonia water, condensed for 6 hours to obtain a wet gel, aged at 40°C for 48 hours, and then placed in a composite treatment solution for modification for 12 hours. The composite treatment solution is prepared by mixing trimethylchlorosilane, ethanol and n-hexane in a ratio of 2:1:8, and then 20% of the total mass of n-hexane is added to the system. The reaction is carried out at 24°C for 6 hours, and then allowed to stand for 24 hours to obtain a composite aerogel.

[0021] S4: Preparation of a microbial agent composition for promoting plant nitrogen absorption The composite bacterial liquid of the soil of the root system of Cymbidium orchid was diluted with 10 times deionized water, and then the composite aerogel was added, with a solid-liquid ratio of 1g:25mL, and then 25% of the total mass of the system of humic acid was added to obtain a microbial agent composition that promotes plant nitrogen absorption.

[0022] Example 2: A preparation process of a microbial agent composition for promoting plant nitrogen absorption, such as Figure 1 As shown, the following steps are included: S1: Preparation of soil compound bacterial solution for the root system of Cymbidium orchid S1.1: Take a well-growing Jianlan plant, put the Jianlan root and 3mm soil around the root into a centrifuge tube, shake and wash the precipitate at 0℃ to obtain a soil suspension.

[0023] S1.2: Add 5 g / L trehalose to the basal culture medium, then add the soil suspension to the basal culture medium and culture at 28°C for 10 days. Pick out the formed strains and transfer them to LB culture medium plates, culture at 37°C for 2 days to obtain the composite bacterial solution of the root soil of Jianlan.

[0024] S2: Preparation and treatment of fiber membrane S2.1: 0.5 parts by mass of polyester particles were dissolved in 4.5 parts by mass of hexafluoroisopropanol, and the polyester spinning solution was obtained by magnetic stirring for 25 hours. The polyester nanofiber membrane was prepared by spinning using an electrospinning device. The voltage of the electrospinning device was 17 kV, the receiving distance was 15 cm, the injection speed was 0.1 mm / min, and the rotation speed was 80 r / min. S2.2: The obtained polyester nanofiber membrane is treated with a vacuum plasma treatment apparatus at a power of 180 W for 25 seconds to obtain a treated fiber membrane.

[0025] S3: Preparation of composite organosilanol solution S3.1: adding 0.2 parts by mass of polyglucosamine to 20 parts by volume of 0.2% acetic acid solution, and stirring with magnetic force at 550 r / min at 60° C. for 35 min to obtain a polyglucosamine solution; S3.2: Add 0.2 parts by mass of the composite bacterial solution from the root soil of Cymbidium orchid to 300 parts by volume of ultrapure water, and stir magnetically at 550 rpm for 15 minutes to obtain a diluted bacterial solution; S3.3: Tetraethyl orthosilicate, ethanol and water were mixed in a volume ratio of 1:2:4, and the pH value was adjusted to 3 with 1 mol / L hydrochloric acid. The mixture was continuously stirred with magnetic stirring at room temperature for 25 h to obtain a silanol solution; S3.4: The polyglucosamine solution and the diluted bacterial solution were mixed in a volume ratio of 2:4, heated to 45°C, and magnetically stirred for 24 hours to obtain a mixed solution. The mixed solution was centrifuged at a speed of 8500 r / min for 10 minutes, and then the supernatant was removed, and the mixture was washed with ultrapure water for 3 times. Then, a silanol solution with a volume equal to that of the polyglucosamine solution was added to obtain a composite organic silanol solution; S3.5: The treated fiber membrane is immersed in a composite organosilanol solution, the pH is adjusted to 6.5 with 0.5 mol / L ammonia water, condensed for 8 hours to obtain a wet gel, aged at 45°C for 50 hours, and then placed in a composite treatment solution for modification for 14 hours. The composite treatment solution is prepared by mixing trimethylchlorosilane, ethanol and n-hexane in a ratio of 2:1:8, and then 20% of the total mass of n-hexane is added to the system. The reaction is carried out at 25°C for 8 hours, and then allowed to stand for 26 hours to obtain a composite aerogel.

[0026] S4: Preparation of a microbial agent composition for promoting plant nitrogen absorption The composite bacterial liquid of the soil of the root system of Cymbidium orchid was diluted with 10 times deionized water, and then the composite aerogel was added, with a solid-liquid ratio of 1g:25mL, and then 25% of the total mass of the system of humic acid was added to obtain a microbial agent composition that promotes plant nitrogen absorption.

[0027] Example 3: A preparation process of a microbial agent composition for promoting nitrogen absorption in plants, such as Figure 1 As shown, the following steps are included: S1: Preparation of soil compound bacterial solution for the root system of Cymbidium orchid S1.1: Take a well-growing Jianlan plant, put the Jianlan root and 3mm soil around the root into a centrifuge tube, shake and wash the precipitate at 0℃ to obtain a soil suspension.

[0028] S1.2: Add 8 g / L of trehalose to the basal culture medium, then add the soil suspension to the basal culture medium and culture at 28°C for 8 days. Pick out the formed strains and transfer them to LB culture medium plates, culture at 37°C for 2 days to obtain the composite bacterial solution of the root soil of Jianlan.

[0029] S2: Preparation and treatment of fiber membrane S2.1: 1 part by mass of polyester particles was dissolved in 5 parts by mass of hexafluoroisopropanol, and the polyester spinning solution was obtained by magnetic stirring for 24 hours. The polyester nanofiber membrane was prepared by spinning using an electrospinning device. The voltage of the electrospinning device was 17 kV, the receiving distance was 15 cm, the injection speed was 0.1 mm / min, and the rotation speed was 80 r / min. S2.2: The obtained polyester nanofiber membrane is treated with a vacuum plasma treatment apparatus at a power of 180 W for 20 seconds to obtain a treated fiber membrane.

[0030] S3: Preparation of composite organosilanol solution S3.1: 0.4 parts by mass of polyglucosamine was added to 25 parts by volume of 0.2% acetic acid solution, and magnetically stirred at 500 rpm for 30 min at 50°C to obtain a polyglucosamine solution; S3.2: Add 0.3 parts by mass of the composite bacterial solution from the root soil of Cymbidium orchid into 320 parts by volume of ultrapure water, and stir magnetically at 500 rpm for 10 minutes to obtain a diluted bacterial solution; S3.3: Tetraethyl orthosilicate, ethanol and water were mixed in a volume ratio of 1:2:5, and the pH value was adjusted to 2 with 1 mol / L hydrochloric acid. The mixture was continuously stirred with magnetic stirring at room temperature for 24 h to obtain a silanol solution; S3.4: The polyglucosamine solution and the diluted bacterial solution were mixed in a volume ratio of 2:5, heated to 40°C, and magnetically stirred for 24 hours to obtain a mixed solution. The mixed solution was centrifuged at a speed of 8000 r / min for 5 minutes, and then the supernatant was removed, and the mixture was washed with ultrapure water for 3 times. Then, a silanol solution with a volume equal to that of the polyglucosamine solution was added to obtain a composite organic silanol solution; S3.5: The treated fiber membrane is immersed in a composite organosilanol solution, the pH is adjusted to 6 with 0.5 mol / L ammonia water, condensed for 6 hours to obtain a wet gel, aged at 40°C for 48 hours, and then placed in a composite treatment solution for modification for 12 hours. The composite treatment solution is prepared by mixing trimethylchlorosilane, ethanol and n-hexane in a ratio of 2:1:8, and then 20% of the total mass of n-hexane is added to the system. The reaction is carried out at 24°C for 6 hours, and then allowed to stand for 24 hours to obtain a composite aerogel.

[0031] S4: Preparation of a microbial agent composition for promoting plant nitrogen absorption The composite bacterial liquid in the soil of the root system of Cymbidium orchid was diluted with 15 times deionized water, and then the composite aerogel was added, with a solid-liquid ratio of 1g:30mL, and then 35% of the total mass of the system was added with humic acid to obtain a microbial agent composition that promotes plant nitrogen absorption.

[0032] Comparative Example 1: Compared with Example 1, Comparative Example 1 is a commercially available humic acid fertilizer.

[0033] Comparative Example 2: Compared with Example 1, the difference of Comparative Example 2 is that no polyglucosamine solution is added in step S3.4, specifically "S3.4: heat the diluted bacterial solution to 40°C, stir magnetically for 24 hours, and then centrifuge at a speed of 8000 r / min for 5 minutes, then remove the supernatant, wash with ultrapure water 3 times, and then add silanol solution, the volume ratio of silanol solution to diluted bacterial solution is 2:4, and obtain a composite organic silanol solution", and the other steps remain unchanged, and the prepared microbial agent composition for promoting plant nitrogen absorption is recorded as Comparative Example 2.

[0034] Comparative Example 3: Compared with Example 1, the difference of Comparative Example 3 is that no silanol solution is added in step S3.4, specifically "the polyglucosamine solution and the diluted bacterial solution are mixed in a volume ratio of 2:4, heated to 40°C, magnetically stirred for 24 hours to obtain a mixed solution, and the mixed solution is centrifuged at a speed of 8000 r / min for 5 minutes, and then the supernatant is removed, and washed with ultrapure water for 3 times to obtain a composite organic solution". The composite organic solution is used instead of the composite organic silanol solution in step S3.5, and the other steps remain unchanged. The prepared microbial agent composition for promoting plant nitrogen absorption is recorded as Comparative Example 3.

[0035] Comparative Example 4: Compared with Example 1, the difference of Comparative Example 4 is that no silanol solution is added in step S3.5, and aerogel is directly prepared, specifically "S3.5: adjust the pH of the composite organic silanol solution to 6 with 0.5 mol / L ammonia water, condense for 6 hours to obtain a wet gel, age at 40°C for 48 hours, and then stand for 24 hours to obtain a composite aerogel", and the other steps remain unchanged, and the prepared microbial agent composition for promoting plant nitrogen absorption is recorded as Comparative Example 4.

[0036] Determination method of total nitrogen content in soil: refer to NY / T 53-1987 "Determination of total nitrogen in soil (semi-micro Kelvin method)", under the action of sodium thiosulfate, concentrated sulfuric acid, perchloric acid and catalyst, all nitrogen is converted into ammonium nitrogen through oxidation-reduction reaction. The ammonia distilled from the alkalized solution after digestion is absorbed by boric acid and titrated with standard hydrochloric acid solution. The total nitrogen content in the soil is calculated according to the amount of standard hydrochloric acid solution used.

[0037] 21 healthy Dendrobium officinale plants were divided into 7 groups, 3 plants in each group, and Examples 1-3 and Comparative Examples 1-4 were sprayed around the root system of each group of Dendrobium officinale, respectively, once every 2 days, for a total of 7 times, with an equal amount of spraying each time. The plants were cultured for 30 days in total, and the total nitrogen content in the soil before and after the culture was tested, as shown in Table 1.

[0038] Table 1 Total soil nitrogen content 30 days ago (mg / kg) Total soil nitrogen content after 30 days (mg / kg) Example 1 15.2±1.3 9.7±0.7 Example 2 15.6±1.3 9.2±0.7 Example 3 14.9±1.3 9.4±0.7 Comparative Example 1 15.3±1.3 11.2±0.7 Comparative Example 2 15.4±1.3 13.7±0.7 Comparative Example 3 15.2±1.3 13.4±0.7 Comparative Example 4 14.7±1.3 12.1±0.7 The total nitrogen content in the soil of Example 1 decreased from 15.2±1.3 mg / kg to 9.7±0.7 mg / kg before and after 30 days, and Example 2 was similar to Example 3. The total nitrogen content in the soil of Comparative Example 1 decreased from 15.3±0.3 mg / kg to 11.2±0.7 mg / kg before and after 30 days, and the degree of decrease in the total nitrogen content in the soil was not as good as that of Examples 1-3. It can be seen that the absorption degree of nitrogen in the soil by the plants in Comparative Example 1 is not as good as that in the examples, and Comparative Example 1 is a humic acid fertilizer, which has a certain function of promoting plant nitrogen absorption. It can be seen that the microbial agent composition of the present invention has a better function of promoting plant nitrogen absorption than commercially available humic acid fertilizers.

[0039] The decrease in total nitrogen content in the soil of Comparative Examples 2-4 before and after 30 days was not as good as that of Examples 1-3 and Comparative Example 1, indicating that the composite aerogel prepared by the composite bacterial liquid of the root soil of Cymbidium orchid with the polyglucosamine solution and the silanol solution has a better ability to enhance the nitrogen absorption of plants.

[0040] The above embodiments are merely illustrative of the principles and effects of the present invention, and are not intended to limit the present invention. Anyone familiar with the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by a person of ordinary skill in the art without departing from the spirit and technical concept disclosed by the present invention shall still be covered by the claims of the present invention.

Claims

1. A preparation process of a microbial agent composition for promoting plant nitrogen absorption, characterized in that: The steps include: S1: Preparation of composite bacterial solution in soil of Jianlan root system Take a well-growing Jianlan plant, put the Jianlan root and the soil around the root into a centrifuge tube, shake and wash the precipitate to obtain a soil suspension, add trehalose to the basic culture medium, and then add the soil suspension to the basic culture medium for cultivation, pick out the formed strain and transfer it to an LB culture medium plate, and cultivate it to obtain a Jianlan root soil composite bacterial liquid; S2: Preparation and treatment of fiber membrane The polyester particles are dissolved in hexafluoroisopropanol, and a polyester spinning solution is obtained by magnetic stirring. An electrostatic spinning device is used to spin and prepare a polyester nanofiber membrane. The obtained polyester nanofiber membrane is treated with a vacuum plasma treatment instrument to obtain a treated fiber membrane. S3: Preparation of composite organosilanol solution Add polyglucosamine to acetic acid solution, stir magnetically to obtain polyglucosamine solution, then add composite bacterial solution of root soil of Cymbidium orchid to ultrapure water to obtain diluted bacterial solution, mix tetraethyl orthosilicate, ethanol and water to obtain silanol solution; mix the polyglucosamine solution and diluted bacterial solution, heat them, stir magnetically to obtain a mixed solution, centrifuge the mixed solution and then remove the supernatant, then add silanol solution to obtain composite organic silanol solution, immerse the treated fiber membrane in the composite organic silanol solution, condense to obtain wet gel, then place in composite treatment solution for modification, and then add n-hexane with the total mass of the system to obtain composite aerogel; S4: Preparation of a microbial agent composition for promoting plant nitrogen absorption The composite bacterial liquid in the soil of the root system of Cymbidium orchid was diluted with 10-15 times of deionized water, and then the composite aerogel was added, with a solid-liquid ratio of 1g: (25-30)mL, and then 25-35% of the total mass of the system of humic acid was added to obtain a microbial agent composition that promotes plant nitrogen absorption.

2. The preparation process of a microbial agent composition for promoting plant nitrogen absorption according to claim 1, characterized in that: Step S1 is to prepare a composite bacterial solution in the soil of the root system of Cymbidium orchid, comprising the following steps: S1.1: Take a well-growing Jianlan plant, put the Jianlan root and 3mm soil around the root into a centrifuge tube, shake and wash the precipitate at 0-4℃ to obtain a soil suspension; S1.2: Add 5-8g / L trehalose to the basal culture medium, then add the soil suspension to the basal culture medium and culture at 28°C for 8-10 days. Pick out the formed strains and transfer them to LB culture medium plates, culture at 37°C for 2 days to obtain the composite bacterial solution of the root soil of Jianlan.

3. The preparation process of a microbial agent composition for promoting plant nitrogen absorption according to claim 2, characterized in that: Step S2 is to prepare and treat the fiber membrane, comprising the following steps: S2.1: dissolving 0.5-1 parts by weight of polyester particles in 4.5-5 parts by weight of hexafluoroisopropanol, stirring magnetically for 24-25 hours to obtain a polyester spinning solution, and spinning with an electrospinning device to prepare a polyester nanofiber membrane; S2.2: The obtained polyester nanofiber membrane is treated with a vacuum plasma treatment apparatus at a power of 180 W for 20-25 s to obtain a treated fiber membrane.

4. The preparation process of a microbial agent composition for promoting plant nitrogen absorption according to claim 3, characterized in that: Step S3 prepares a composite organosilanol solution, comprising the following steps: S3.1: adding 0.2-0.4 parts by mass of polyglucosamine to 20-25 parts by volume of 0.2% acetic acid solution, and stirring with a magnetic force of 500-550 r / min at 50-60° C. for 30-35 min to obtain a polyglucosamine solution; S3.2: Add 0.2-0.3 parts by mass of the composite bacterial solution from the root soil of Cymbidium orchid into 300-320 parts by volume of ultrapure water, and stir magnetically at 500-550 r / min for 10-15 min to obtain a diluted bacterial solution; S3.3: Mix tetraethyl orthosilicate, ethanol and water in a volume ratio of 1:2:(4-5), adjust the pH to 2-3 with 1-1.5 mol / L hydrochloric acid, and stir continuously under magnetic stirring at room temperature for 24-25 h to obtain a silanol solution; S3.4: The polyglucosamine solution and the diluted bacterial solution were mixed in a volume ratio of 2:(4-5), heated to 40-45°C, and magnetically stirred for 24 hours to obtain a mixed solution. The mixed solution was centrifuged at a speed of 8000-8500 r / min for 5-10 minutes, and then the supernatant was removed, and the mixture was washed with ultrapure water for 3 times. Then, a silanol solution with an equal volume to the polyglucosamine solution was added to obtain a composite organic silanol solution; S3.5: Immerse the treated fiber membrane in a composite organosilicone solution, adjust the pH to 6-6.5 with 0.5 mol / L ammonia water, perform polycondensation for 6-8 hours to obtain a wet gel, age it at 40-45°C for 48-50 hours, and then place it in the composite treatment solution for modification for 12-14 hours. Then add 20-25% of the total mass of n-hexane in the system, react at 24-26°C for 6-8 hours, and then let it stand for 24-26 hours to obtain a composite aerogel.

5. The preparation process of a microbial agent composition for promoting plant nitrogen absorption according to claim 3, characterized in that: The voltage of the electrospinning equipment was 17 kV, the receiving distance was 15 cm, the injection speed was 0.1 mm / min, and the rotation speed was 80 r / min.

6. The preparation process of a microbial agent composition for promoting plant nitrogen absorption according to claim 4, characterized in that: The composite treatment liquid is prepared by mixing trimethylchlorosilane, ethanol and n-hexane in a ratio of 2:1:

8.

7. A microbial agent composition for promoting plant nitrogen absorption, which is prepared by the preparation process of the microbial agent composition for promoting plant nitrogen absorption according to any one of claims 1 to 6.

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