Automatic dyeing equipment for biological tissue sample slices

Abstract

The invention provides automatic dyeing equipment for biological tissue sample slices, which comprises a feeding mechanism, a feeding and discharging buffering mechanism, an automatic dyeing mechanism, a reagent adding mechanism, an after-dyeing drying mechanism, a reagent storage mechanism and a main console, the main console is in control connection with the feeding mechanism, the feeding and discharging buffering mechanism, the automatic dyeing mechanism, the reagent adding mechanism, the after-dyeing drying mechanism and the reagent storage mechanism, the feeding mechanism is provided with a plurality of microscope glass slide vertical placing supports, the automatic dyeing mechanism is provided with a dyeing groove, and the dyeing groove can be filled with a dyeing reagent and is matched with the microscope glass slides; the reagent adding mechanism can automatically add and supplement a dyeing reagent to the dyeing tank, the reagent storage mechanism can add and supplement the dyeing reagent to the reagent adding mechanism, and the post-dyeing drying mechanism is matched with the feeding and discharging temporary storage mechanism and the automatic dyeing mechanism. The microscope glass slide has the beneficial effect that the risk of cross contamination between microscope glass slides filled with biological tissue sample slices can be avoided.

Description

Technical Field

[0001] The invention relates to the technical field of biological tissue sample slice staining, and in particular to an automatic staining device for biological tissue sample slices. Background Art

[0002] In the existing biological sample analysis technology, the analysis methods commonly used include microscopic analysis, microarray analysis (e.g., protein and nucleic acid microarray analysis) and mass spectrometry. Preparing samples for these and other types of analysis usually includes: contacting the biological sample with a series of processing liquids. Some of these processing liquids (e.g., staining reagents and counterstaining reagents) can add colors and, in contrast or on the contrary, change the visual characteristics of invisible or difficult-to-see sample components (e.g., at least some types of cells and intracellular structures). Other processing liquids (e.g., deparaffinized liquids) can be used to achieve other processing purposes. If a variety of processing liquids are used to process the sample, the application and subsequent removal of various processing liquids can be very important for producing samples suitable for analysis. In some cases, processing samples with a variety of processing liquids includes: manually applying the processing liquids to microscope slides that carry the samples respectively. However, this method of manually processing samples tends to be labor-intensive and imprecise, so biological tissue sample section staining equipment has emerged.

[0003] The automatic staining and processing technology of biological tissue sample sections is an important step in the entire process of automated staining, drying and sealing of biological tissue samples. It is also an indispensable step. In order to make the processed biological tissue samples have an appearance that is easy to observe and to maintain the consistency and efficiency of the processing process, the staining step is an object that must be strictly controlled during the processing of biological tissue samples. The purpose of staining is to make different structures in cell tissues present different colors for easy observation. The classic hematoxylin and eosin (eosin) staining method is a routine staining for histological specimens and pathological section specimens, referred to as HE staining. After staining, the cell nucleus is stained purple-blue by hematoxylin, and most cytoplasm and non-cellular components are stained pink by eosin.

[0004] Existing biological tissue sample section staining equipment can replace manual staining. These machines automatically process samples by immersing racks carrying batches of microscope slides in an open bath of processing liquid. However, the existing biological tissue sample section staining equipment places batches of microscope slides containing biological tissue sample sections horizontally in a slide carrier box. Although there is a certain upper and lower space interval between each microscope slide, a whole box of microscope slides is processed together during the immersion staining operation. When the intervals are immersed in a common staining reagent, it is inevitable that there is a risk of cross-contamination between microscope slides in the same box. For example, cells of biological tissue sample sections can leave the sample on one microscope slide and move to another microscope slide in the common staining reagent. This form of contamination greatly reduces the accuracy of certain types of sample analysis. Moreover, after a whole box of horizontally placed microscope slides are immersed in a common staining reagent, the osmotic pressure of the staining reagent applied to the upper microscope slide is lower than that applied to the lower microscope slide, inevitably leading to the problem of uneven staining of the upper and lower microscope slides, resulting in poor product quality consistency of the final stained biological tissue sample section microscope slides, and bringing a very bad user experience. Summary of the invention

[0005] In order to solve the problems in the prior art, the present invention provides an automatic staining device for biological tissue sample slices. By arranging a loading mechanism, a loading and unloading buffer mechanism, an automatic staining mechanism, a reagent adding mechanism, a post-staining drying mechanism, a reagent storage mechanism and a main control console that cooperate with each other in the automatic staining device for biological tissue sample slices, the microscope slide vertical support can evenly separate each microscope slide when loading, and the staining slot of the automatic staining reaction tray can separate and isolate each microscope slide for staining operations, which can avoid the risk of cross contamination between microscope slides containing biological tissue sample slices, improve the product quality of biological tissue sample slice microscope slides, improve user experience, and solve the problems of cross contamination risk and poor product consistency when the biological tissue sample slice staining device in the prior art is used for staining.

[0006] The present invention provides an automatic staining device for biological tissue sample slices, comprising a loading mechanism, a loading and unloading buffer mechanism, an automatic staining mechanism, a reagent adding mechanism, a drying mechanism after staining, a reagent storage mechanism and a main control console, wherein the main control console is controlled and connected with the loading mechanism, the loading and unloading buffer mechanism, the automatic staining mechanism, the reagent adding mechanism, the drying mechanism after staining and the reagent storage mechanism, the loading mechanism, the loading and unloading buffer mechanism, the automatic staining mechanism, the reagent adding mechanism, the drying mechanism after staining and the reagent storage mechanism are all fixedly arranged on a workbench, the loading mechanism cooperates with the loading and unloading buffer mechanism and the automatic staining mechanism, the loading mechanism is provided with a plurality of microscope slide vertical placement brackets, wherein the microscope slide vertical placement brackets can vertically and fixedly place a plurality of microscope slides in an array and each of the microscope slides The slides are separated from each other, the automatic staining mechanism is provided with an automatic staining reaction tray, the automatic staining reaction tray is provided with a plurality of staining slots for staining operations of single microscope slides, the staining slots can be filled with staining reagents and are compatible with microscope slides, the reagent adding mechanism can automatically add supplementary staining reagents to the staining slots, the reagent storage mechanism can add supplementary staining reagents to the reagent adding mechanism, the post-staining drying mechanism cooperates with the loading and unloading buffer mechanism and the automatic staining mechanism, the loading mechanism can clamp the microscope slide in the microscope slide vertical placement bracket on the loading and unloading buffer mechanism and move it into the staining slot for staining operations, and after the microscope slide completes the staining operation in the staining slot, is dried in the post-staining drying mechanism, is put back into the microscope slide vertical placement bracket, and then is discharged through the loading and unloading buffer mechanism.

[0007] The present invention is further improved, the loading mechanism includes a translation feeding component, a sample slice feeding lifting component, a sample slice feeding heating cavity, a sample slice feeding identification camera and a microscope slide vertical support, the translation feeding component, the sample slice feeding lifting component and the sample slice feeding heating cavity are all fixedly arranged on the workbench, the translation feeding component is provided with a translation feeding platform, the translation feeding platform can place a plurality of the microscope slide vertical supports, the sample slice feeding lifting component cooperates with the translation feeding platform, the sample slice feeding heating cavity is arranged above the sample slice feeding lifting component, the sample slice feeding lifting component can be moved between the translation feeding platform and the sample slice The sample slice feeding and heating chamber reciprocates, the sample slice feeding identification camera is arranged on the side of the second translation guide rail and cooperates with the second translation guide rail, the microscope slide in the microscope slide vertical placement bracket is provided with label information, the sample slice feeding identification camera can identify the label information of the microscope slide and feed it back to the main control console of the biological tissue sample slice staining device, the translation feeding component can transport the microscope slide vertical placement bracket with multiple biological tissue sample slice microscope slides to the sample slice feeding lifting component, and is transferred to the sample slice feeding heating chamber by the sample slice feeding lifting component for heating and melting wax, and then transported to the next process through the translation feeding component.

[0008] The present invention is further improved, the translation feeding assembly also includes a first translation bracket and a first translation guide rail, the bottom of the first translation bracket is fixedly arranged on the workbench, the bottom of the first translation guide rail is fixedly arranged on the first translation bracket, and the bottom of the translation feeding platform is slidably connected to the first translation guide rail; the translation feeding assembly also includes a second translation guide rail, one end of the second translation guide rail is located below the sample slice feeding heating chamber and cooperates with the sample slice feeding lifting assembly, and the other end of the second translation guide rail extends to the biological tissue sample slice The next process of the staining equipment; the sample slice feeding lifting assembly includes a sample slice feeding lifting bracket, a sample slice feeding lifting guide rail and a sample slice feeding lifting platform, the bottom of the sample slice feeding lifting bracket is fixedly set on the workbench, the sample slice feeding lifting guide rail is fixedly vertically set on the side of the first translation bracket, and the side of the sample slice feeding lifting platform is slidably connected to the sample slice feeding lifting guide rail; a molten wax feeding opening is provided at the bottom of the sample slice feeding heating cavity, and the molten wax feeding opening is adapted to the sample slice feeding lifting platform.

[0009] The present invention is further improved, the loading and unloading cache mechanism includes a cache feeding component, a microscope slide detection camera and a cache discharging component, the cache feeding component and the cache discharging component are fixedly arranged on the workbench in sequence along the loading direction of the loading mechanism, the cache feeding component is provided with a cache feeding platform, the cache feeding platform can place a plurality of microscope slide vertical brackets in an array, the microscope slide vertical bracket can vertically and fixedly place a plurality of microscope slides in an array and each of the microscope slides is spaced apart, the cache feeding platform cooperates with the loading mechanism, the cache feeding platform can temporarily store a plurality of microscope slide vertical brackets transmitted by the loading mechanism; the cache feeding component also includes a cache feeding bracket, the bottom of the cache feeding bracket is fixedly arranged on the workbench, the bottom of the cache feeding platform is fixedly arranged on the cache feeding bracket, the bottom of the cache feeding platform is also provided with a cache feeding drive motor, and the cache feeding The material driving motor can drive the microscope slide vertical bracket located on the cache feeding platform to move; the cache discharging assembly is provided with a cache discharging platform, the cache discharging platform can place multiple microscope slide vertical brackets in an array, and the cache discharging platform cooperates with the loading mechanism; the cache discharging assembly also includes a cache discharging bracket, the bottom of the cache discharging bracket is fixedly arranged on the workbench, the bottom of the cache discharging platform is fixedly arranged on the cache discharging bracket, and the bottom of the cache discharging platform is also provided with a cache discharging driving motor, the cache discharging driving motor can drive the microscope slide vertical bracket located on the cache discharging platform to move to complete the discharging, the microscope slide detection camera is arranged on one side of the loading mechanism and cooperates with the cache feeding platform, the microscope slide detection camera can identify the microscope slide in the microscope slide vertical bracket and feed back to the main control console of the biological tissue sample section staining device.

[0010] The present invention is further improved, the automatic staining mechanism also includes an automatic staining slide feeding clamping component, a staining tank cleaning component, a slide discharging cleaning component and an automatic staining slide discharging clamping component, the automatic staining slide feeding clamping component, the automatic staining reaction tray, and the automatic staining slide discharging clamping component are all fixedly arranged on a workbench, the main control console of the biological tissue sample section staining device is controlled and connected with the automatic staining slide feeding clamping component, the automatic staining reaction tray, and the automatic staining slide discharging clamping component, the automatic staining slide feeding clamping component and the automatic staining slide discharging clamping component are both coordinated with the loading and unloading buffer mechanism, and the loading and unloading buffer mechanism can place multiple microscope slides vertically in an array. A bracket is placed, a reaction disc rotating shaft is provided in the middle of the automatic staining reaction disc, the automatic staining reaction disc is fixedly connected to the top of the reaction disc rotating shaft, a reaction disc rotating drive motor is provided at the bottom of the reaction disc rotating shaft, the reaction disc rotating drive motor is fixedly arranged on a workbench, the reaction disc rotating drive motor is rotationally driven connected to the reaction disc rotating shaft, the staining tank cleaning component cooperates with the automatic staining reaction disc, the main control console of the biological tissue sample section staining equipment is control-connected to the staining tank cleaning component, the staining tank cleaning component can extract the staining reagent in the staining tank, the slide discharging cleaning component cooperates with the automatic staining slide discharging manipulator, and the slide discharging cleaning component can clean the microscope slide that has completed the staining operation.

[0011] The present invention is further improved, the automatic staining slide feeding and clamping assembly includes an automatic staining slide feeding motor, an automatic staining slide feeding shaft and an automatic staining slide feeding manipulator, the automatic staining slide feeding motor is fixedly arranged on the workbench, the automatic staining slide feeding motor is rotationally driven connected to the bottom of the automatic staining slide feeding shaft, the top of the automatic staining slide feeding shaft is fixedly connected to the top of the automatic staining slide feeding manipulator, the automatic staining slide feeding manipulator can move between the loading and unloading buffer mechanism and the automatic staining reaction tray Reciprocating motion; the automatic staining glass slide discharging and clamping assembly includes an automatic staining glass slide discharging motor, an automatic staining glass slide discharging shaft and an automatic staining glass slide discharging manipulator, the automatic staining glass slide discharging motor is fixedly arranged on the workbench, the automatic staining glass slide discharging motor is rotationally driven connected to the bottom of the automatic staining glass slide discharging shaft, the top of the automatic staining glass slide discharging shaft is fixedly connected to the top of the automatic staining glass slide discharging manipulator, and the automatic staining glass slide discharging manipulator can reciprocate between the loading and unloading buffer mechanism and the automatic staining reaction tray.

[0012] The present invention is further improved, the reagent adding mechanism includes an automatic staining reagent adding component, a staining reagent adding base and a staining reagent temporary storage component, the bottom of the staining reagent adding base and the bottom of the staining reagent temporary storage component are fixedly arranged on the workbench, the staining reagent temporary storage component is located next to the staining reagent adding base and cooperates with the automatic staining reagent adding component, the main control console of the biological tissue sample section staining device is controlled and connected with the automatic staining reagent adding component, the automatic staining reagent adding component includes a staining reagent adding driving device, a staining reagent extraction and release mechanical arm, a staining reagent adding shaft and a staining reagent adding turntable, the staining reagent adding turntable is movably arranged on the staining reagent adding base, the bottom of the staining reagent adding shaft is fixedly connected to the staining reagent adding turntable, the top of the staining reagent adding shaft is fixedly connected to one end of the staining reagent extraction and release mechanical arm, the staining reagent adding driving device is rotationally driven and connected to the staining reagent adding turntable, the staining reagent extraction and release mechanical arm can extract the staining reagent in the staining reagent temporary storage component and move to the staining tank under the control instruction of the main control console and the drive of the staining reagent adding driving device to add supplementary staining reagent to the staining tank.

[0013] The present invention is further improved, the dyeing reagent adding drive device includes a dyeing reagent adding drive motor and a dyeing reagent adding transmission belt, the dyeing reagent adding drive motor is connected to the dyeing reagent adding turntable for rotational driving through the dyeing reagent adding transmission belt; the dyeing reagent temporary storage component includes a plurality of dyeing reagent temporary storage tanks arranged in an array, the top of the dyeing reagent temporary storage tank is open and cooperates with the dyeing reagent extraction and release robotic arm, the capacity of the dyeing reagent temporary storage tank is greater than 50ml, the number of the dyeing reagent temporary storage tanks is 8, and the number of the dyeing reagent automatic adding components is 2 groups.

[0014] The present invention is further improved, the post-staining drying mechanism comprises a slide drying tank, a drying base and a heating component, the bottom of the drying base is fixedly arranged on the workbench, the slide drying tank is fixedly arranged on the drying base, the heating component is arranged closely around the surface of the slide drying tank and cooperates with the slide drying tank, the main control console of the biological tissue sample section staining device is controlled and connected with the heating component, the internal space of the slide drying tank is adapted to the microscope slide, the loading and unloading buffer mechanism can place a plurality of microscope slide vertical brackets in an array, the microscope slide vertical bracket can vertically and fixedly place a plurality of microscope slides in an array and each of the microscope slides The slides are separated from each other, the automatic dyeing mechanism can clamp the microscope slide in the microscope slide vertical support on the loading and unloading buffer mechanism for dyeing, and after the microscope slide completes the dyeing operation, it is moved into the slide drying tank for drying and then put back into the microscope slide vertical support and then discharged through the loading and unloading buffer mechanism. The slide drying tank is a vertical rectangular block as a whole, the top of the slide drying tank is open and the internal space is a rectangular storage space, the rectangular storage space of the slide drying tank can accommodate at least one microscope slide, and the heating component includes a heating resistor, and the heating resistor is tightly arranged around the surface of the slide drying tank.

[0015] The present invention is further improved, the reagent storage mechanism includes a clean water storage component, a clean water pumping component, a staining reagent storage component and a staining reagent pumping component, the clean water storage component, the clean water pumping component, the staining reagent storage component and the staining reagent pumping component are all fixedly arranged on a workbench, the main control console of the biological tissue sample section staining device is controlled and connected with the clean water pumping component and the staining reagent pumping component, the clean water storage component is connected with the clean water pumping component, the clean water pumping component cooperates with the staining tank cleaning component and the glass slide discharge cleaning component, the staining reagent storage component is connected with the staining reagent pumping component, the staining reagent pumping component cooperates with the staining reagent temporary storage component, the staining reagent storage component includes a plurality of staining reagent storage containers arranged in an array, the staining reagent storage containers are connected with the staining reagent pumping component, the clean water pumping component can extract the clean water in the clean water storage component to the staining tank cleaning component and the glass slide discharge cleaning component, and the staining reagent pumping component can extract the staining The staining reagent in the reagent storage container is supplied to the staining reagent temporary storage component for replenishing the staining reagent for the staining tank. The clean water storage component is a water storage tank, and the capacity of the water storage tank is not less than 2L. The clean water pumping component includes a diaphragm pump and a two-way valve. The main control console of the biological tissue sample section staining device is controlled and connected with the diaphragm pump. One end of the diaphragm pump is communicated with the bottom of the side of the water storage tank, and the other end of the diaphragm pump is communicated with the staining tank cleaning component and the slide discharge cleaning component. The two-way valve is located between the diaphragm pump and the staining tank cleaning component and the slide discharge cleaning component. The number of the staining reagent storage containers is 8, and the capacity of the staining reagent storage containers is greater than 1L. The staining reagent pumping component includes a quantitative pump and a three-way valve. The main control console of the biological tissue sample section staining device is controlled and connected with the quantitative pump. One end of the quantitative pump is respectively communicated with the bottom of the side of the 8 staining reagent storage containers, and the other end of the quantitative pump is respectively communicated with two groups of the staining reagent temporary storage components. The three-way valve is located between the quantitative pump and the staining reagent temporary storage component.

[0016] Compared with the prior art, the beneficial effects of the present invention are as follows: the present invention provides an automatic staining device for biological tissue sample slices, by arranging a loading mechanism, a loading and unloading buffer mechanism, an automatic staining mechanism, a reagent adding mechanism, a post-staining drying mechanism, a reagent storage mechanism and a main control console that cooperate with each other in the automatic staining device for biological tissue sample slices, the loading mechanism can clamp the microscope slide in the microscope slide vertical placement bracket on the loading and unloading buffer mechanism and move it into the staining tank for staining operation, and after the microscope slide completes the staining operation in the staining tank and is dried in the post-staining drying mechanism, it is put back into the microscope slide vertical placement bracket and then discharged through the loading and unloading buffer mechanism, and the microscope slide vertical placement bracket can place each microscope slide when loading. The uniform separation avoids the risk of cross-contamination between microscope slides containing biological tissue sample slices. The staining slot of the automatic staining reaction tray can separate and isolate each microscope slide for staining operations, and can also avoid the risk of cross-contamination between microscope slides containing biological tissue sample slices, thereby improving the product quality of biological tissue sample slice microscope slides. The reagent adding mechanism can automatically add supplementary staining reagents to the staining slot without manual operation, which saves time and effort and is not prone to errors. The production efficiency of biological tissue sample slice microscope slides is greatly improved, the user experience is improved, and the problems of cross-contamination risks and poor product consistency when staining biological tissue sample slice staining equipment in the prior art are solved. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a structural diagram of the automatic staining device for biological tissue sample slices of the present invention;

[0018] Figure 2 It is a structural diagram of the feeding mechanism of the present invention;

[0019] Figure 3 It is a structural diagram of the loading and unloading buffer mechanism of the present invention;

[0020] Figure 4 It is a structural diagram of the loading and unloading buffer mechanism of the present invention;

[0021] Figure 5 It is a structural diagram of the automatic dyeing mechanism of the present invention;

[0022] Figure 6 It is a structural diagram of the reagent adding mechanism of the present invention;

[0023] Figure 7 It is a structural diagram of the drying mechanism after dyeing of the present invention;

[0024] Figure 8 It is a structural diagram of the reagent storage mechanism of the present invention.

[0025] In the figure, 1-feeding mechanism, 11-translational feeding component, 111-translational feeding platform, 112-first translation bracket, 12-sample slice feeding lifting component, 121-sample slice feeding lifting bracket, 13-sample slice feeding heating chamber, 14-microscope slide vertical bracket, 15-sample slice feeding recognition camera, 2-up and down material buffer mechanism, 21-buffer feeding component, 211-buffer feeding platform, 212-buffer feeding bracket, 213-buffer feeding drive motor, 22-buffer discharging component, 221-buffer discharging platform, 222-buffer discharging bracket, 223-buffer discharging drive motor, 23-microscope slide detection camera, 3-automatic staining mechanism, 31-automatic staining slide feeding clamping component, 32-automatic Dynamic staining reaction plate, 321-staining trough, 33-automatic staining slide discharging and clamping assembly, 35-staining trough cleaning assembly, 36-slide discharging and cleaning assembly, 4-reagent adding mechanism, 41-staining reagent automatic adding assembly, 411-staining reagent adding driving device, 412-staining reagent extraction and release mechanical arm, 413-staining reagent adding rotating shaft, 414-staining reagent adding turntable, 42-staining reagent adding base, 43-staining reagent temporary storage assembly, 431-staining reagent temporary storage tank, 5-post-staining drying mechanism, 51-slide drying trough, 52-drying base, 53-heating assembly, 6-reagent storage mechanism, 61-clean water storage assembly, 62-clean water pumping assembly, 63-staining reagent storage assembly, 64-staining reagent pumping assembly. DETAILED DESCRIPTION

[0026] The present invention is further described in detail below with reference to the accompanying drawings and embodiments.

[0027] like Figure 1-Figure 8As shown, an automatic staining device for biological tissue sample sections provided by the present invention comprises a feeding mechanism 1, a loading and unloading buffer mechanism 2, an automatic staining mechanism 3, a reagent adding mechanism 4, a post-staining drying mechanism 5, a reagent storage mechanism 6 and a main control console, the main control console is controlled and connected with the feeding mechanism 1, the loading and unloading buffer mechanism 2, the automatic staining mechanism 3, the reagent adding mechanism 4, the post-staining drying mechanism 5, and the reagent storage mechanism 6, the feeding mechanism 1, the loading and unloading buffer mechanism 2, the automatic staining mechanism 3, the reagent adding mechanism 4, the post-staining drying mechanism 5, and the reagent storage mechanism 6 are all fixedly arranged on a workbench, the feeding mechanism 1 cooperates with the loading and unloading buffer mechanism 2 and the automatic staining mechanism 3, and the feeding mechanism 1 is provided with a plurality of microscope slide vertical supports 14, in which a plurality of microscope slides can be vertically fixedly placed in an array and each microscope slide is separated from each other, an automatic staining mechanism 3 is provided with an automatic staining reaction tray 32, and the automatic staining reaction tray 32 is provided with a plurality of staining slots 321 for staining operations of a single microscope slide, and the staining slots 321 can be filled with staining reagents and are adapted to the microscope slides, a reagent adding mechanism 4 can automatically add supplementary staining reagents to the staining slots 321, a reagent storage mechanism 6 can add supplementary staining reagents to the reagent adding mechanism 4, and a drying mechanism 5 after staining cooperates with the loading and unloading buffer mechanism 2 and the automatic staining mechanism 3. In the present embodiment, the loading mechanism can clamp the microscope slide in the microscope slide vertical holder on the loading and unloading buffer mechanism and move it into the staining tank for staining operation. After the microscope slide completes the staining operation in the staining tank and is dried in the post-staining drying mechanism, it is put back into the microscope slide vertical holder and then unloaded through the loading and unloading buffer mechanism. The microscope slide vertical holder can evenly separate each microscope slide during loading, thus avoiding the risk of cross contamination between microscope slides with biological tissue sample slices. The staining tank of the automatic staining reaction tray can separate each microscope slide for staining operation, thus avoiding the risk of cross contamination between microscope slides with biological tissue sample slices, thus improving the product quality of biological tissue sample slice microscope slides. The reagent adding mechanism can automatically add supplementary staining reagent to the staining tank without manual operation, thus saving time and effort and being less prone to errors. This greatly improves the production efficiency of biological tissue sample slice microscope slide products and improves the user experience.

[0028] like Figure 2As shown, the loading mechanism 1 includes a translation feeding component 11, a sample slice feeding lifting component 12, a sample slice feeding heating cavity 13 and a microscope slide vertical support 14. The translation feeding component 11, the sample slice feeding lifting component 12 and the sample slice feeding heating cavity 13 are all fixedly arranged on the workbench. The translation feeding component 11 is provided with a translation feeding platform 111. The translation feeding platform 111 can place a plurality of microscope slide vertical supports 14. The microscope slide vertical support 14 can vertically and fixedly place a plurality of microscope slides in an array and each microscope slide is separated from each other. The sample slice feeding lifting component 12 cooperates with the translation feeding platform 111. The sample slice feeding heating cavity 13 is arranged above the sample slice feeding lifting component 12. The sample slice feeding lifting component 12 can reciprocate between the translation feeding platform 111 and the sample slice feeding heating cavity 13. In this embodiment, the translational feeding assembly can transport the microscope slide vertical support on which a plurality of biological tissue sample slice microscope slides are placed to the sample slice feeding lifting assembly, and after being transferred by the sample slice feeding lifting assembly to the sample slice feeding heating chamber for heating and melting wax, the microscope slide vertical support can be evenly separated from each microscope slide during loading, thereby avoiding the risk of cross contamination between microscope slides containing biological tissue sample slices, and each vertically placed microscope slide is immersed in a common staining reagent and is subjected to the same osmotic pressure of the staining reagent, so that the quality consistency of the biological tissue sample slice microscope slide products is better, thereby improving the product quality of the biological tissue sample slice microscope slide products and improving the user experience.

[0029] like Figure 2As shown, the translation feeding assembly 11 also includes a first translation bracket 112 and a first translation guide rail. The bottom of the first translation bracket 112 is fixedly arranged on the workbench, the bottom of the first translation guide rail is fixedly arranged on the first translation bracket 112, and the bottom of the translation feeding platform 111 is slidably connected in the first translation guide rail. The translation feeding assembly 11 also includes a second translation guide rail, one end of which is located below the sample slice feeding heating cavity 13 and cooperates with the sample slice feeding lifting assembly, and the other end of the second translation guide rail extends to the next process of the biological tissue sample slice staining device. In this embodiment, the first translation guide rail is used to transport the microscope slide vertical bracket to the sample slice feeding lifting platform for heating and wax melting. After the wax melting operation is completed, the microscope slide vertical bracket is transported to the next process of the biological tissue sample slice staining device through the second translation guide rail. The sample slice feeding lifting assembly 12 includes a sample slice feeding lifting bracket 121, a sample slice feeding lifting guide rail and a sample slice feeding lifting platform. The bottom of the sample slice feeding lifting bracket 121 is fixedly set on the workbench, the sample slice feeding lifting guide rail is fixedly and vertically set on the side of the first translation bracket, the side of the sample slice feeding lifting platform is slidably connected to the sample slice feeding lifting guide rail, and a molten wax feeding opening is provided at the bottom of the sample slice feeding heating cavity 13, and the molten wax feeding opening is adapted to the sample slice feeding lifting platform; it also includes a sample slice feeding identification camera 15, which is set on the side of the second translation guide rail and cooperates with the second translation guide rail. The microscope slide in the microscope slide vertical placement bracket is provided with label information. The sample slice feeding identification camera can identify the label information of the microscope slide and feed it back to the main control console of the biological tissue sample slice staining device. In this embodiment, the sample slice feeding lifting assembly is used to transport the microscope slide vertical bracket to the sample slice feeding heating chamber for heating and wax melting operation. After the wax melting operation is completed, the microscope slide vertical bracket is transported to the translation feeding assembly through the sample slice feeding lifting guide rail and the sample slice feeding lifting platform, and then transported to the next process of the biological tissue sample slice staining equipment by the translation feeding assembly. During this process, the sample slice feeding identification camera will identify the label information of the microscope slide and feed it back to the biological tissue sample slice staining equipment.

[0030] like Figure 3-Figure 4As shown, the loading and unloading cache mechanism includes a cache feeding component 21 and a cache discharging component 22. The cache feeding component 21 and the cache discharging component 22 are fixedly arranged on the workbench in sequence along the loading direction of the loading mechanism 1. The cache feeding component 21 is provided with a cache feeding platform 211. The cache feeding platform 211 can place multiple microscope slide vertical brackets in an array. The microscope slide vertical bracket can place multiple microscope slides vertically in an array and each microscope slide is separated from each other. The cache feeding platform 211 cooperates with the loading mechanism 1. The cache feeding component 21 also includes a cache feeding bracket 212. The bottom of the cache feeding bracket 212 is fixedly arranged on the workbench. The bottom of the cache feeding platform 211 is fixedly arranged on the cache feeding bracket 212. The bottom of the cache feeding platform 211 is also provided with a cache feeding drive motor 213. The cache feeding drive motor 213 can drive the microscope slide vertical bracket located on the cache feeding platform 211 to move. In this embodiment, the cache feeding platform 211 can temporarily store multiple microscope slide vertical racks transmitted by the loading mechanism, and after the microscope slides in the microscope slide vertical racks are stained, they are put back into the microscope slide vertical racks and moved to the loading mechanism 1, and then transported to the cache discharging component 22 through the loading mechanism 1 to complete the discharging. The cache platform greatly improves the processing efficiency of the entire biological tissue sample section staining equipment and improves the user experience.

[0031] like Figure 3-Figure 4As shown, the cache discharging component 22 is provided with a cache discharging platform 221, which can place multiple microscope slide vertical brackets in an array, and the cache discharging platform 221 cooperates with the feeding mechanism 1. The cache discharging component 22 also includes a cache discharging bracket 222, the bottom of which is fixedly arranged on the workbench, and the bottom of the cache discharging platform 221 is fixedly arranged on the cache discharging bracket 222. The bottom of the cache discharging platform 221 is also provided with a cache discharging drive motor 223, and the cache discharging drive motor 223 can drive the microscope slide vertical bracket located on the cache discharging platform 221 to move to complete the discharging. It also includes a microscope slide detection camera 23, which is arranged on one side of the feeding mechanism 1 and cooperates with the cache feeding platform 211. The microscope slide detection camera 23 can identify the microscope slide in the microscope slide vertical bracket and feed it back to the main control console of the biological tissue sample section staining device. In this embodiment, when the loading mechanism transports the microscope slide vertical rack containing microscope slides, the microscope slide detection camera can identify the microscope slides in the microscope slide vertical rack and feed back to the main control console of the biological tissue sample section staining device, and the microscope slides are clamped out of the microscope slide vertical rack for staining. Then the buffer feeding drive motor pushes the microscope slide vertical rack to move on the buffer feeding platform to facilitate the storage of more microscope slide vertical racks. After the staining operation is completed, the microscope slides are inserted back into the microscope slide vertical rack and then transported to the buffer discharging platform via the loading mechanism. The buffer discharging drive motor pushes the microscope slide vertical rack to complete the discharging operation.

[0032] like Figure 5As shown, the automatic staining mechanism includes an automatic staining slide feeding clamping component 31, an automatic staining reaction tray 32 and an automatic staining slide discharging clamping component 33. The automatic staining slide feeding clamping component 31, the automatic staining reaction tray 32 and the automatic staining slide discharging clamping component 33 are all fixedly arranged on a workbench. The main control console of the biological tissue sample section staining device is controlled and connected with the automatic staining slide feeding clamping component 31, the automatic staining reaction tray 32 and the automatic staining slide discharging clamping component 33. The automatic staining reaction tray 32 is provided with a plurality of staining slots 321 for staining operations of a single microscope slide. The staining slots 321 can be filled with staining reagents and are connected to the microscope. The automatic staining glass slide feeding clamping component 31 and the automatic staining glass slide discharging clamping component 32 are both matched with the loading and unloading buffer mechanism 2. The loading and unloading buffer mechanism 2 can place multiple microscope slide vertical brackets in an array. The microscope slide vertical bracket can vertically and fixedly place multiple microscope slides in an array and each microscope slide is separated from each other. A reaction disc rotating shaft is provided in the middle of the automatic staining reaction disc 32. The automatic staining reaction disc 32 is fixedly connected to the top of the reaction disc rotating shaft. A reaction disc rotating drive motor is provided at the bottom of the reaction disc rotating shaft. The reaction disc rotating drive motor is fixedly set on the workbench, and the reaction disc rotating drive motor is connected to the reaction disc rotating shaft for rotation driving. In this embodiment, the automatic staining slide feeding and clamping assembly can clamp the microscope slide in the microscope slide vertical holder on the loading and unloading buffer mechanism and move it into the staining tank for staining operation, and after the microscope slide is stained, it is put back into the microscope slide vertical holder and then discharged through the loading and unloading buffer mechanism. The staining tank of the automatic staining reaction tray can separate each microscope slide for staining operation, avoiding the risk of cross contamination between microscope slides containing biological tissue sample slices, and each microscope slide immersed in the common staining reagent is subjected to the same osmotic pressure of the staining reagent. Finally, the quality consistency of the biological tissue sample slice microscope slide product is better, which improves the product quality of the biological tissue sample slice microscope slide and improves the user experience.

[0033] like Figure 5As shown, the automatic staining slide feeding and clamping assembly 31 includes an automatic staining slide feeding motor, an automatic staining slide feeding shaft and an automatic staining slide feeding manipulator. The automatic staining slide feeding motor is fixedly arranged on the workbench, the automatic staining slide feeding motor is rotationally driven connected to the bottom of the automatic staining slide feeding shaft, the top of the automatic staining slide feeding shaft is fixedly connected to the top of the automatic staining slide feeding manipulator, and the automatic staining slide feeding manipulator can reciprocate between the loading and unloading buffer mechanism 2 and the automatic staining reaction tray 32. In this embodiment, the automatic staining slide feeding robot can, driven by the automatic staining slide feeding motor, clamp the microscope slide in the vertical bracket on the loading and unloading buffer mechanism and move it into the staining tank for staining operation; the automatic staining slide discharging clamping assembly 33 includes an automatic staining slide discharging motor, an automatic staining slide discharging shaft and an automatic staining slide discharging robot. The automatic staining slide discharging motor is fixedly arranged on the workbench, the automatic staining slide discharging motor is connected to the bottom of the automatic staining slide discharging shaft for rotation driving, the top of the automatic staining slide discharging shaft is fixedly connected to the top of the automatic staining slide discharging robot, and the automatic staining slide discharging robot can reciprocate between the loading and unloading buffer mechanism 2 and the automatic staining reaction tray 32. In this embodiment, the automatic staining slide discharging manipulator can clamp the microscope slide that has completed the staining operation in the staining tank and move it into the microscope slide vertical support on the loading and unloading buffer mechanism under the drive of the automatic staining slide discharging motor; it also includes a staining tank cleaning component 35, which cooperates with the automatic staining reaction disk 32, and the main control console of the biological tissue sample section staining device is controlled and connected with the staining tank cleaning component 35, and the staining tank cleaning component 35 can extract the staining reagent in the staining tank 321. In this embodiment, the staining tank cleaning component can extract the staining reagent in the staining tank according to the control instruction of the main control console of the biological tissue sample section staining device; it also includes a slide discharging cleaning component 36, which cooperates with the automatic staining slide discharging manipulator, and the slide discharging cleaning component 36 can clean the microscope slide that has completed the staining operation. In this embodiment, the slide discharging and cleaning component is filled with cleaning reagents, and the microscope slides that have completed the staining operation can be cleaned by the slide discharging and cleaning component before being discharged. During the staining operation, the microscope slides can be placed in the slide discharging and cleaning component for cleaning once after each staining operation in the staining tank, and then placed in the next staining tank for staining according to the staining process.

[0034] like Figure 6As shown, the reagent adding mechanism includes a staining reagent automatic adding component 41, a staining reagent adding base 42 and a staining reagent temporary storage component 43. The bottom of the staining reagent adding base 42 and the bottom of the staining reagent temporary storage component 43 are fixedly arranged on the workbench. The staining reagent temporary storage component 43 is located next to the staining reagent adding base 42 and cooperates with the staining reagent automatic adding component 41. The main control console of the biological tissue sample section staining device is controlled and connected with the staining reagent automatic adding component 41. The staining reagent automatic adding component 41 includes a staining reagent adding driving device 411, a staining reagent extraction and placement mechanical arm 412, a staining reagent adding rotating shaft 413 and a staining reagent The dyeing reagent adding turntable 414 is movably arranged on the dyeing reagent adding base 42, the bottom of the dyeing reagent adding shaft 413 is fixedly connected to the dyeing reagent adding turntable 414, the top of the dyeing reagent adding shaft 413 is fixedly connected to one end of the dyeing reagent extracting and releasing mechanical arm 412, the dyeing reagent adding driving device 411 is connected to the dyeing reagent adding turntable 414 for rotational driving, the dyeing reagent adding driving device 411 includes a dyeing reagent adding driving motor and a dyeing reagent adding transmission belt, and the dyeing reagent adding driving motor is connected to the dyeing reagent adding turntable 414 for rotational driving through the dyeing reagent adding transmission belt. In this embodiment, the dyeing reagent extracting and releasing mechanical arm can extract the dyeing reagent in the dyeing reagent temporary storage component and move to the dyeing tank under the control instruction of the main console and the drive of the dyeing reagent adding driving device to add a supplementary dyeing reagent to the dyeing tank, which can realize the automatic addition of supplementary dyeing reagent to the dyeing tank without manual operation, saving time and effort and not prone to error, greatly improving the production efficiency and product quality of the biological tissue sample slice microscope slide product, and improving the user experience. The staining reagent temporary storage component 43 includes a plurality of staining reagent temporary storage tanks 431 arranged in an array, the top of the staining reagent temporary storage tank 431 is open and cooperates with the staining reagent extraction and discharge mechanical arm 412, the capacity of the staining reagent temporary storage tank 431 is greater than 50ml, the number of the staining reagent temporary storage tank 431 is 8, and the number of the staining reagent automatic addition component 41 is 2 groups. In the present embodiment, each staining reagent temporary storage tank can temporarily store 50ml staining reagent for the staining reagent extraction and discharge mechanical arm to extract and use, and can temporarily store up to 8 kinds of staining reagents, which is convenient for automatic addition and replenishment to the staining tank. The reagent adding mechanism 4 cooperates with the automatic staining reaction disk 32, and the main control console of the biological tissue sample slice staining device is controlled and connected with the reagent adding mechanism 4, and the reagent adding mechanism 4 can add staining reagents into the staining tank 321. In the present embodiment, the reagent adding mechanism can add staining reagents into the staining tank according to the control instructions of the main control console of the biological tissue sample slice staining device.

[0035] like Figure 7As shown, the post-staining drying mechanism includes a slide drying tank 51, a drying base 52 and a heating component 53. The bottom of the drying base 53 is fixedly set on the workbench, the slide drying tank 51 is fixedly set on the drying base 52, the heating component 53 is tightly arranged around the outer surface of the slide drying tank 51 and cooperates with the slide drying tank 51, the main control console of the biological tissue sample section staining equipment is controlled and connected with the heating component 53, the internal space of the slide drying tank 51 is adapted to the microscope slide, the loading and unloading buffer mechanism 2 can place a plurality of microscope slide vertical brackets in an array, and the microscope slide vertical bracket can vertically and fixedly place a plurality of microscope slides in an array and each microscope slide is separated from each other. In this embodiment, the automatic dyeing mechanism can clamp the microscope slide in the microscope slide vertical holder on the loading and unloading buffer mechanism for dyeing, and after the microscope slide is stained, it is moved into the slide drying tank for drying and then put back into the microscope slide vertical holder and then discharged through the loading and unloading buffer mechanism. The slide drying tank can accelerate the drying of the microscope slide after dyeing, saving the time of static drying, greatly improving the production efficiency of the biological tissue sample slice microscope slide product, and improving the user experience. The slide drying tank 51 is a vertically placed rectangular parallelepiped as a whole. The top of the slide drying tank 51 is open and the internal space is a rectangular parallelepiped storage space. The rectangular parallelepiped storage space of the slide drying tank 51 can accommodate at least one microscope slide. The heating component 53 includes a heating resistor, and the heating resistor is arranged closely around the surface of the slide drying tank. In this embodiment, the heating component can control the heating resistor to generate heat according to the control instructions of the main control console, thereby heating the slide drying tank. After drying and dyeing, the microscope slide is placed in the slide drying tank and finally put back into the microscope slide vertical bracket and then discharged through the loading and unloading buffer mechanism.

[0036] like Figure 8As shown, the reagent storage mechanism includes a clean water storage component 61, a clean water pumping component 62, a staining reagent storage component 63 and a staining reagent pumping component 64. The clean water storage component 61, the clean water pumping component 62, the staining reagent storage component 63 and the staining reagent pumping component 64 are all fixedly arranged on the workbench. The main control console of the biological tissue sample section staining device is controlled and connected with the clean water pumping component 62 and the staining reagent pumping component 64. The clean water storage component 61 is connected with the clean water pumping component 62. The clean water pumping component 62 cooperates with the staining tank cleaning component 35 and the slide discharge cleaning component 36. The staining reagent storage component 63 is connected with the staining reagent pumping component 64. The staining reagent pumping component 64 cooperates with the staining reagent temporary storage component 43. The staining reagent storage component 63 includes a plurality of staining reagent storage containers arranged in an array, and the staining reagent storage containers are connected with the staining reagent pumping component 64. In this embodiment, the clean water pumping component can extract the clean water in the clean water storage component to the staining tank cleaning component and the slide discharge cleaning component, and the staining reagent pumping component can extract the staining reagent in the staining reagent storage container to the staining reagent temporary storage component to replenish the staining reagent for the staining tank, and can automatically add the supplementary staining reagent to the staining reagent temporary storage component. The staining reagent in the staining reagent temporary storage component can automatically replenish the staining tank without manual operation, saving time and effort and not prone to error, greatly improving the production efficiency and product quality of the biological tissue sample slice microscope slide product, and improving the user experience. The clean water storage component is a water storage tank, and the capacity of the water storage tank is not less than 2L. The clean water pumping component includes a diaphragm pump and a two-way valve. The main control console of the biological tissue sample slice staining device is connected to the diaphragm pump control, one end of the diaphragm pump is connected to the bottom of the side of the water storage tank, and the other end of the diaphragm pump is connected to the staining tank cleaning component 35 and the slide discharge cleaning component 336. The two-way valve is located between the diaphragm pump and the staining tank cleaning component 35 and the slide discharge cleaning component 36. In this embodiment, the diaphragm pump can extract clean water from the water storage tank according to the control instructions of the main control console and pump clean water to the staining tank cleaning component and the slide discharge cleaning component for cleaning the staining tank and the microscope slide, and the two-way valve serves as a switch for pumping clean water. The number of staining reagent storage containers is 8, and the capacity of the staining reagent storage container is greater than 1L. The staining reagent pumping component 64 includes a quantitative pump and a three-way valve. The main control console of the biological tissue sample section staining device is connected to the quantitative pump control. One end of the quantitative pump is connected to the bottom of the side of the 8 staining reagent storage containers, and the other end of the quantitative pump is connected to two groups of staining reagent temporary storage components 43, respectively. The three-way valve is located between the quantitative pump and the staining reagent temporary storage component 43.In this embodiment, the metering pump can quantitatively extract the dyeing reagent in the dyeing reagent storage container and quantitatively pump the dyeing reagent to the dyeing reagent temporary storage component according to the control instructions of the main control console. The dyeing reagent temporary storage component is provided with 8 dyeing reagent temporary storage tanks with a capacity greater than 50 ml. Each dyeing reagent storage container corresponds to a dyeing reagent temporary storage tank in a group of dyeing reagent temporary storage components, which is used for the dyeing reagent temporary storage tank to automatically add supplementary dyeing reagent to the dyeing tank. The three-way valve serves as a switch for pumping the dyeing reagent channel.

[0037] As can be seen from the above, the present invention provides an automatic staining device for biological tissue sample sections. By arranging a loading mechanism, a loading and unloading buffer mechanism, an automatic staining mechanism, a reagent adding mechanism, a post-staining drying mechanism, a reagent storage mechanism and a main control console that cooperate with each other in the automatic staining device for biological tissue sample sections, the loading mechanism can clamp the microscope slide in the microscope slide vertical placement bracket on the loading and unloading buffer mechanism and move it into the staining tank for staining operation. After the microscope slide completes the staining operation in the staining tank and is dried in the post-staining drying mechanism, it is put back into the microscope slide vertical placement bracket and then discharged through the loading and unloading buffer mechanism. The microscope slide vertical placement bracket can evenly separate each microscope slide when loading, avoiding The cross-contamination risk between microscope slides containing biological tissue sample slices is eliminated. The staining slot of the automatic staining reaction tray can separate and isolate each microscope slide for staining operations, and can also avoid the cross-contamination risk between microscope slides containing biological tissue sample slices, thereby improving the product quality of biological tissue sample slice microscope slides. The reagent adding mechanism can automatically add supplementary staining reagents to the staining slot without manual operation, saving time and effort and being less prone to errors. The production efficiency of biological tissue sample slice microscope slides is greatly improved, the user experience is improved, and the problems of cross-contamination risks and poor product consistency when staining biological tissue sample slice staining equipment in the prior art are solved.

[0038] The specific implementation modes described above are preferred implementation modes of the present invention, and are not intended to limit the specific implementation scope of the present invention. The scope of the present invention includes but is not limited to the specific implementation modes, and all equivalent changes made according to the present invention are within the protection scope of the present invention.

Claims

1. An automatic staining device for biological tissue sample sections, characterized in that: The present invention comprises a feeding mechanism, a loading and unloading buffer mechanism, an automatic dyeing mechanism, a reagent adding mechanism, a drying mechanism after dyeing, a reagent storage mechanism and a main control console, wherein the main control console is controlled and connected with the feeding mechanism, the loading and unloading buffer mechanism, the automatic dyeing mechanism, the reagent adding mechanism, the drying mechanism after dyeing and the reagent storage mechanism, the feeding mechanism, the loading and unloading buffer mechanism, the automatic dyeing mechanism, the reagent adding mechanism, the drying mechanism after dyeing and the reagent storage mechanism are all fixedly arranged on a workbench, the feeding mechanism cooperates with the loading and unloading buffer mechanism and the automatic dyeing mechanism, the feeding mechanism is provided with a plurality of microscope slide vertical mounting brackets, in which a plurality of microscope slides can be vertically fixedly arranged in an array and each of the microscope slides is spaced apart, the automatic dyeing The color mechanism is provided with an automatic staining reaction tray, which is provided with a plurality of staining slots for staining a single microscope slide. The staining slot can be filled with staining reagents and is compatible with the microscope slides. The reagent adding mechanism can automatically add supplementary staining reagents to the staining slot, and the reagent storage mechanism can add supplementary staining reagents to the reagent adding mechanism. The post-staining drying mechanism cooperates with the loading and unloading buffer mechanism and the automatic staining mechanism. The loading mechanism can clamp the microscope slide in the microscope slide vertical placement bracket on the loading and unloading buffer mechanism and move it into the staining slot for staining. After the microscope slide completes the staining operation in the staining slot and is dried in the post-staining drying mechanism, it is put back into the microscope slide vertical placement bracket and then discharged through the loading and unloading buffer mechanism.

2. The automatic staining device for biological tissue sample sections according to claim 1, characterized in that: The feeding mechanism includes a translation feeding component, a sample slice feeding lifting component, a sample slice feeding heating cavity, a sample slice feeding identification camera and a microscope slide vertical support. The translation feeding component, the sample slice feeding lifting component and the sample slice feeding heating cavity are all fixedly arranged on a workbench. The translation feeding component is provided with a translation feeding platform, and the translation feeding platform can place a plurality of microscope slide vertical supports. The sample slice feeding lifting component cooperates with the translation feeding platform. The sample slice feeding heating cavity is arranged above the sample slice feeding lifting component. The sample slice feeding lifting component can be moved between the translation feeding platform and the sample slice feeding heating cavity. The sample slice feeding identification camera is arranged on the side of the second translation guide rail and cooperates with the second translation guide rail. The microscope slide in the microscope slide vertical placement bracket is provided with label information. The sample slice feeding identification camera can identify the label information of the microscope slide and feed it back to the main control console of the biological tissue sample slice staining device. The translation feeding component can transport the microscope slide vertical placement bracket with multiple biological tissue sample slice microscope slides to the sample slice feeding lifting component, and is transferred to the sample slice feeding heating cavity by the sample slice feeding lifting component for heating and melting wax, and then transported to the next process through the translation feeding component.

3. The automatic staining device for biological tissue sample sections according to claim 2, characterized in that: The translation feeding assembly also includes a first translation bracket and a first translation guide rail, the bottom of the first translation bracket is fixedly arranged on the workbench, the bottom of the first translation guide rail is fixedly arranged on the first translation bracket, and the bottom of the translation feeding platform is slidably connected to the first translation guide rail; the translation feeding assembly also includes a second translation guide rail, one end of the second translation guide rail is located below the sample slice feeding heating chamber and cooperates with the sample slice feeding lifting assembly, and the other end of the second translation guide rail extends to the next process of the biological tissue sample slice staining device; the sample slice feeding lifting assembly includes a sample slice feeding lifting bracket, a sample slice feeding lifting guide rail and a sample slice feeding lifting platform, the bottom of the sample slice feeding lifting bracket is fixedly arranged on the workbench, the sample slice feeding lifting guide rail is fixedly and vertically arranged on the side of the first translation bracket, and the side of the sample slice feeding lifting platform is slidably connected to the sample slice feeding lifting guide rail; a melting wax feeding opening is provided at the bottom of the sample slice feeding heating chamber, and the melting wax feeding opening is adapted to the sample slice feeding lifting platform.

4. The automatic staining device for biological tissue sample sections according to claim 3, characterized in that: The loading and unloading cache mechanism includes a cache feeding component, a microscope slide detection camera and a cache discharging component. The cache feeding component and the cache discharging component are fixedly arranged on the workbench in sequence along the loading direction of the loading mechanism. The cache feeding component is provided with a cache feeding platform. The cache feeding platform can place a plurality of microscope slide vertical brackets in an array. The microscope slide vertical bracket can place a plurality of microscope slides in an array vertically and fixedly and each of the microscope slides is spaced apart. The cache feeding platform cooperates with the loading mechanism. The cache feeding platform can temporarily store a plurality of microscope slide vertical brackets transmitted by the loading mechanism. The cache feeding component also includes a cache feeding bracket. The bottom of the cache feeding bracket is fixedly arranged on the workbench. The bottom of the cache feeding platform is fixedly arranged on the cache feeding bracket. The bottom of the cache feeding platform is also provided with a cache feeding drive motor. It is capable of driving the microscope slide vertical support located on the cache feeding platform to move; the cache discharging assembly is provided with a cache discharging platform, the cache discharging platform is capable of placing a plurality of microscope slide vertical supports in an array, and the cache discharging platform cooperates with the loading mechanism; the cache discharging assembly also includes a cache discharging support, the bottom of the cache discharging support is fixedly arranged on the workbench, the bottom of the cache discharging platform is fixedly arranged on the cache discharging support, and the bottom of the cache discharging platform is also provided with a cache discharging driving motor, the cache discharging driving motor is capable of driving the microscope slide vertical support located on the cache discharging platform to move to complete the discharging, the microscope slide detection camera is arranged on one side of the loading mechanism and cooperates with the cache feeding platform, the microscope slide detection camera is capable of identifying the microscope slide in the microscope slide vertical support and feeding back to the main control console of the biological tissue sample section staining device.

5. The automatic staining device for biological tissue sample sections according to claim 4, characterized in that: The automatic staining mechanism also includes an automatic staining slide feeding and clamping component, a staining tank cleaning component, a slide discharging and cleaning component, and an automatic staining slide discharging and clamping component. The automatic staining slide feeding and clamping component, the automatic staining reaction tray, and the automatic staining slide discharging and clamping component are all fixedly arranged on a workbench. The main control console of the biological tissue sample section staining device is controlled and connected with the automatic staining slide feeding and clamping component, the automatic staining reaction tray, and the automatic staining slide discharging and clamping component. The automatic staining slide feeding and clamping component and the automatic staining slide discharging and clamping component are both coordinated with the loading and unloading caching mechanism. The loading and unloading caching mechanism can place multiple microscope slide vertical brackets in an array. A reaction disc rotating shaft is provided in the middle of the automatic staining reaction disc, the automatic staining reaction disc is fixedly connected to the top of the reaction disc rotating shaft, a reaction disc rotating drive motor is provided at the bottom of the reaction disc rotating shaft, the reaction disc rotating drive motor is fixedly arranged on a workbench, the reaction disc rotating drive motor is rotationally driven connected to the reaction disc rotating shaft, the staining tank cleaning component cooperates with the automatic staining reaction disc, the main control console of the biological tissue sample section staining equipment is control-connected to the staining tank cleaning component, the staining tank cleaning component can extract the staining reagent in the staining tank, the slide discharging cleaning component cooperates with the automatic staining slide discharging manipulator, and the slide discharging cleaning component can clean the microscope slide that has completed the staining operation.

6. The automatic staining device for biological tissue sample sections according to claim 5, characterized in that: The automatic staining slide feeding and clamping assembly comprises an automatic staining slide feeding motor, an automatic staining slide feeding shaft and an automatic staining slide feeding manipulator, the automatic staining slide feeding motor is fixedly arranged on a workbench, the automatic staining slide feeding motor is rotationally driven connected to the bottom of the automatic staining slide feeding shaft, the top of the automatic staining slide feeding shaft is fixedly connected to the top of the automatic staining slide feeding manipulator, and the automatic staining slide feeding manipulator can reciprocate between the loading and unloading buffer mechanism and the automatic staining reaction tray; The automatic staining glass slide discharging and clamping assembly includes an automatic staining glass slide discharging motor, an automatic staining glass slide discharging shaft and an automatic staining glass slide discharging manipulator. The automatic staining glass slide discharging motor is fixedly arranged on a workbench, the automatic staining glass slide discharging motor is rotationally driven connected to the bottom of the automatic staining glass slide discharging shaft, the top of the automatic staining glass slide discharging shaft is fixedly connected to the top of the automatic staining glass slide discharging manipulator, and the automatic staining glass slide discharging manipulator can reciprocate between the loading and unloading buffer mechanism and the automatic staining reaction tray.

7. The automatic staining device for biological tissue sample sections according to claim 6, characterized in that: The reagent adding mechanism comprises an automatic staining reagent adding component, a staining reagent adding base and a staining reagent temporary storage component. The bottom of the staining reagent adding base and the bottom of the staining reagent temporary storage component are fixedly arranged on the workbench. The staining reagent temporary storage component is located next to the staining reagent adding base and cooperates with the automatic staining reagent adding component. The main control console of the biological tissue sample section staining device is controlled and connected with the automatic staining reagent adding component. The automatic staining reagent adding component comprises a staining reagent adding driving device, a staining reagent extraction and release mechanical arm, a staining reagent adding shaft and a staining reagent adding turntable. The staining reagent adding turntable is movably arranged on the staining reagent adding base. The bottom of the staining reagent adding shaft is fixedly connected to the staining reagent adding turntable. The top of the staining reagent adding shaft is fixedly connected to one end of the staining reagent extraction and release mechanical arm. The staining reagent adding driving device is rotationally driven and connected to the staining reagent adding turntable. The staining reagent extraction and release mechanical arm can extract the staining reagent in the staining reagent temporary storage component and move to the staining tank under the control instruction of the main control console and the drive of the staining reagent adding driving device to add supplementary staining reagent to the staining tank.

8. The automatic staining device for biological tissue sample sections according to claim 7, characterized in that: The dyeing reagent adding driving device comprises a dyeing reagent adding driving motor and a dyeing reagent adding transmission belt, and the dyeing reagent adding driving motor is connected to the dyeing reagent adding turntable for rotational driving via the dyeing reagent adding transmission belt; the dyeing reagent temporary storage component comprises a plurality of dyeing reagent temporary storage tanks arranged in an array, the top of the dyeing reagent temporary storage tank is open and cooperates with the dyeing reagent extraction and release robotic arm, the capacity of the dyeing reagent temporary storage tank is greater than 50ml, the number of the dyeing reagent temporary storage tanks is 8, and the number of the dyeing reagent automatic adding components is 2 groups.

9. The automatic staining device for biological tissue sample sections according to claim 8, characterized in that: The post-staining drying mechanism includes a slide drying tank, a drying base and a heating component. The bottom of the drying base is fixedly arranged on a workbench, the slide drying tank is fixedly arranged on the drying base, the heating component is arranged closely around the surface of the slide drying tank and cooperates with the slide drying tank, the main control console of the biological tissue sample section staining device is controlled and connected with the heating component, the internal space of the slide drying tank is adapted to the microscope slide, the loading and unloading buffer mechanism can place a plurality of microscope slide vertical brackets in an array, and the microscope slide vertical bracket can vertically and fixedly place a plurality of microscope slides in an array and each of the microscope slides is spaced The automatic dyeing mechanism can clamp the microscope slide in the microscope slide vertical support on the loading and unloading buffer mechanism for dyeing, and after the microscope slide is dyed, it is moved into the slide drying tank for drying and then put back into the microscope slide vertical support and then discharged through the loading and unloading buffer mechanism. The slide drying tank is a vertical rectangular block as a whole. The top of the slide drying tank is open and the internal space is a rectangular storage space. The rectangular storage space of the slide drying tank can accommodate at least one microscope slide. The heating component includes a heating resistor, and the heating resistor is tightly arranged around the surface of the slide drying tank.

10. The automatic staining device for biological tissue sample sections according to claim 9, characterized in that: The reagent storage mechanism includes a clean water storage component, a clean water pumping component, a staining reagent storage component and a staining reagent pumping component. The clean water storage component, the clean water pumping component, the staining reagent storage component and the staining reagent pumping component are all fixedly arranged on a workbench. The main control console of the biological tissue sample section staining device is controlled and connected with the clean water pumping component and the staining reagent pumping component. The clean water storage component is connected with the clean water pumping component. The clean water pumping component cooperates with the staining tank cleaning component and the glass slide discharge cleaning component. The staining reagent storage component is connected with the staining reagent pumping component. The staining reagent pumping component cooperates with the staining reagent temporary storage component. The staining reagent storage component includes a plurality of staining reagent storage containers arranged in an array. The staining reagent storage container is connected with the staining reagent pumping component. The clean water pumping component can extract clean water in the clean water storage component to the staining tank cleaning component and the glass slide discharge cleaning component. The staining reagent pumping component can extract clean water from the staining reagent storage container. The staining reagent in the device is supplied to the staining reagent temporary storage component to replenish the staining reagent for the staining tank, the clean water storage component is a water storage tank, the capacity of the water storage tank is not less than 2L, the clean water pumping component includes a diaphragm pump and a two-way valve, the main control console of the biological tissue sample section staining device is controlled and connected with the diaphragm pump, one end of the diaphragm pump is communicated with the bottom of the side of the water storage tank, the other end of the diaphragm pump is communicated with the staining tank cleaning component and the slide discharge cleaning component, the two-way valve is located between the diaphragm pump and the staining tank cleaning component and the slide discharge cleaning component, the number of the staining reagent storage containers is 8, the capacity of the staining reagent storage containers is greater than 1L, the staining reagent pumping component includes a quantitative pump and a three-way valve, the main control console of the biological tissue sample section staining device is controlled and connected with the quantitative pump, one end of the quantitative pump is respectively communicated with the bottom of the side of the 8 staining reagent storage containers, the other end of the quantitative pump is respectively communicated with two groups of the staining reagent temporary storage components, and the three-way valve is located between the quantitative pump and the staining reagent temporary storage component.

Images