Marine pollutant identification equipment applying laser-induced fluorescence and working method thereof

Abstract

The invention relates to the field of marine pollution detection, in particular to marine pollutant identification equipment applying laser-induced fluorescence and a working method thereof.The marine pollutant identification equipment comprises a laser emitter, a supporting device, a reflecting mirror arranged on the supporting device, a sample pool, an induction device, a spectrograph and a central computer; laser emitted by the laser emitter is refracted by the reflecting mirror and then enters the sample pool, a first angle deflection device for adjusting the deflection angle of the reflecting mirror is arranged on the supporting device, and a second angle deflection device for further deflecting the laser into the sensing device is arranged in the sample pool. And the laser filtered by the sensing device is input into the spectrograph through the optical fiber, and the result is displayed in the central computer, so that the technical problem of experiment failure caused by deflection of a light path in the operation process in the existing marine pollutant laser identification process is solved.

Description

Technical Field

[0001] The invention relates to the field of marine pollution detection, and in particular to a marine pollutant identification device using laser induced fluorescence and a working method thereof. Background Art

[0002] Today, marine pollution mainly includes plastic pollution and chemical pollution. Plastic pollution includes about 8 million tons of plastic entering the ocean every year, which accounts for 80% of the total marine garbage. Plastic garbage is solid garbage that is visible to the naked eye and can be cleaned directly, but chemical pollution is that pollutants are directly integrated with seawater and are difficult to distinguish with the naked eye. Therefore, professional instruments are needed to take seawater samples for testing, such as the latest use of laser induced fluorescence for marine pollutant identification in seawater pollution detection.

[0003] Laser induced fluorescence is a spectral method that excites atoms or molecules to a higher energy level by absorbing laser light and then emits light spontaneously. This technology has the advantages of rapidity, high precision, high sensitivity, and non-destructive testing, and is widely used in medicine, environment, biology, chemical industry, food and other fields.

[0004] The Chinese patent application number 202310751572.2 is an algae-based laser-induced fluorescence detection device, which specifically relates to the technical field of algae concentration detection devices, including a base, a lifting device is fixedly installed at the rear of the upper end of the base, a connecting plate is fixedly installed at the output end of the lifting device, and a two-dimensional regulator is fixedly installed at the lower part of the connecting plate extending forward, a semiconductor laser is fixedly installed at the lower end of the two-dimensional regulator, a quartz cylindrical lens is arranged outside the laser port of the semiconductor laser, and a sliding device is arranged on the base. The algae-based laser-induced fluorescence detection device described in the invention drives the optical glass cuvette to move through the sliding device, and cooperates with the two-dimensional regulator to make the laser beam emitted by the semiconductor laser vertically irradiate the quartz cylindrical lens, and then vertically irradiate the optical glass cuvette container after the quartz cylindrical lens expands the beam in the two-dimensional plane, with accurate positioning, improved measurement accuracy, and reduced experimental errors. The technical solution proposed in the above invention is conducive to the miniaturization of the device through structural improvements, and can also avoid the laser causing background noise to the device. However, in actual use, since the laser transmitter needs to adjust the optical path through a reflector to shoot the laser into the sample pool with high precision, the adjustment of the reflector directly affects whether the optical path of the laser is accurate. The above invention obviously does not make improvements on this key step, which can easily lead to the shaking of the reflector during use, causing the optical path to be deflected and thus causing the experiment to fail. Summary of the invention

[0005] Therefore, in order to solve the above problems, the present invention proposes a marine pollutant identification device using laser induced fluorescence and a working method thereof, which solves the technical problem of experimental failure caused by the deflection of the light path during operation in the existing marine pollutant laser identification process.

[0006] To achieve the above-mentioned purpose, the present invention adopts the following technical scheme: a marine pollutant identification device using laser induced fluorescence, comprising a laser transmitter, a supporting device, a reflector arranged on the supporting device, a sample pool, a sensing device, a spectrometer and a central computer, wherein the laser transmitter emits laser light which enters the sample pool after being refracted by the reflector, the supporting device is provided with a first angle deflection device for adjusting the deflection angle of the reflector, the sample pool is provided with a second angle deflection device for further deflecting the laser light into the sensing device, the laser light filtered by the sensing device is input into the spectrometer through an optical fiber and the result is presented in the central computer.

[0007] Furthermore, the supporting device includes a supporting base, a supporting rod fixedly connected to the supporting base, and a functional plate fixedly arranged on the top of the supporting rod.

[0008] Furthermore, the first angle deflection device includes a first connecting rod, a second connecting rod, and a rotating wheel movably connected to the first connecting rod and the second connecting rod respectively; a functional cavity is provided in the functional disk, and the first angle deflection device is provided in the functional cavity.

[0009] Furthermore, the first connecting rod and the second connecting rod are parallel to each other and perpendicular to the horizontal plane, the first connecting rod is fixedly provided with a first protrusion, the second connecting rod is fixedly provided with a second protrusion, the functional cavity is provided with a first elongated hole and a second elongated hole, the first elongated hole and the second elongated hole both penetrate to the outer surface of the functional disk, the first protrusion extends out of the functional cavity via the first elongated hole, the second protrusion extends out of the functional cavity via the second elongated hole, the two ends of the reflector are respectively hinged to the first protrusion and the second protrusion, and the reflector is an assembled mirror.

[0010] Furthermore, a first meshing tooth is provided on the first connecting rod, a second meshing tooth is provided on the second connecting rod, a third meshing tooth is provided on the outer circumferential side of the rotating wheel, the first connecting rod and the second connecting rod are provided on both sides of the rotating wheel, and the third meshing tooth is respectively meshed with the first meshing tooth and the second meshing tooth.

[0011] Furthermore, the rotating wheel includes a coaxially arranged rotating inner wheel and a rotating outer wheel, the rotating inner wheel is arranged in the functional cavity and connected to the first connecting rod and the second connecting rod, the rotating outer wheel extends outward to the outer surface of the functional disk, and the rotating outer wheel and the rotating inner wheel are connected by an elastic telescopic rod, and the rotating outer wheel is driven to rotate by external force, thereby driving the rotating inner wheel to rotate.

[0012] Furthermore, through the elastic action of the elastic telescopic rod, the rotating outer wheel moves away from the rotating inner wheel under the action of external force or approaches the rotating inner wheel in a power-losing state, and a first positioning tooth is arranged on the outer surface of the functional disk and is surrounded by the rotating outer wheel, and a second positioning tooth is arranged on the outer circumferential surface of the rotating outer wheel, and the first positioning tooth and the second positioning tooth are meshed with each other. When an external force is applied to the rotating outer wheel to move it away from the rotating inner wheel, the first positioning tooth and the second positioning tooth are separated from each other, and when the rotating outer wheel loses power and approaches the rotating inner wheel, the first positioning tooth and the second positioning tooth are engaged with each other.

[0013] Furthermore, the second angle deflection device includes three plane mirrors spliced ​​to each other, each of the plane mirrors has the same specifications and is in the shape of an isosceles right triangle, and the right-angled sides of two adjacent plane mirrors are connected to form a pyramid space.

[0014] Furthermore, the sensing device includes a receiving probe and a filter for receiving the laser beam after passing through the second angle deflection device in the sample cell.

[0015] A working method of a marine pollutant identification device using laser induced fluorescence comprises the following steps: S1. Seawater sampling: sampling seawater from seawater and placing it in a sample pool; S2, emitting laser: using a 405nm laser emitter for laser emission, the emitted laser beam enters the sample pool after being reflected once by the reflector; S3, secondary reflection; the laser light entering the sample cell passes through the second angle deflection device and then is emitted vertically upward to the sensing device; S4, laser filtering; the filtered laser is poured into the spectrometer via optical fiber and the data is then transmitted to the central computer; S5. Data analysis: The central computer analyzes and processes the data and displays it on the display screen.

[0016] By adopting the above technical solution, the beneficial effects of the present invention are: 1. The present invention detects marine pollutants by using a laser-induced fluorescence scheme. The principle is that a 405-nanometer laser transmitter is used to emit laser light, which is reflected by a reflector into a sample pool and then further reflected into a sensing device. A filter is provided in the sensing device to filter out 405-nanometer blue light. Then, the oil in the seawater sample will produce fluorescence under laser irradiation and further be displayed in a central computer through a spectrometer, thereby achieving the detection of the pollution degree of the seawater sample according to the waveform.

[0017] 2. The present invention adopts a first angle deflection device and a second angle deflection device to ensure that the laser emitted from the laser emitter can pass through the sample cell and then be introduced into the spectrometer for waveform presentation, wherein the first angle deflection device is arranged on the supporting device, and is used to adjust the spatial position of the reflector on the supporting device. The first angle deflection device includes a first connecting rod, a second connecting rod and a rotating wheel. The principle is that the first connecting rod and the second connecting rod are respectively arranged at both ends of the rotating wheel, and both are meshed and connected with the rotating wheel. In this way, as long as the rotating wheel is rotated, the first connecting rod and the second connecting rod will move in opposite directions, such as the first connecting rod rises, the second connecting rod falls, or the first connecting rod moves down. The first connecting rod descends and the second connecting rod rises. At the same time, a first protrusion is provided on the first connecting rod and a second protrusion is provided on the second connecting hole. The head and tail ends of the reflector are respectively hinged with the first protrusion and the second protrusion. In this way, when the first connecting rod and the second connecting rod move in opposite directions, the angle of the reflector will be deflected. The deflection angle of the reflector can be adjusted by rotating the rotating wheel in a forward or reverse rotation manner. At the same time, the reflector adopts an assembled mirror to ensure that the first connecting rod and the second connecting rod will not interfere with the deflection of the reflector during the up and down lifting process, that is, when the reflector is in a horizontal state, the reflector is completely retracted and is in a state of shortest length, and slowly extends when it is deflected.

[0018] 3. In the present invention, the first meshing teeth, the second meshing teeth and the third meshing teeth are provided, and the three mesh with each other, so that the reflector can be adjusted more accurately with the rotation of the rotating wheel, and the laser deflection angle can be further accurately adjusted.

[0019] 4. The rotating wheel in the present invention comprises a coaxially arranged rotating inner wheel and a rotating outer wheel, the purpose of which is to ensure that after the angle of the reflector is adjusted, it will not be disturbed by the outside world and will not deviate from itself. The principle is that the rotating inner wheel and the rotating outer wheel are movably connected by an elastic telescopic rod, that is, the rotating outer wheel can be pulled outward and then rotated to drive the rotating inner wheel to rotate, and when the rotating outer wheel loses power, it will retract inward, and after retraction, since a first positioning tooth is provided on the outer circumferential surface of the functional disk and a second positioning tooth is provided on the outer circumferential surface of the rotating outer wheel, the rotating outer wheel can be locked by the mutual engagement of the first positioning tooth and the second positioning tooth, thereby ensuring that the rotating inner wheel is locked, and then ensuring that the states of the first connecting rod and the second connecting rod are fixedly locked, thereby ensuring that the state of the reflector is fixed and will not be disturbed by the outside world.

[0020] 5. The second angle deflection device in the present invention is responsible for reflecting the laser light incident into the sample pool and emitting it perpendicularly to the horizontal plane to be received by the sensing device. The second angle deflection device includes three plane mirrors spliced ​​to each other, and the shapes of the three plane mirrors are isosceles right triangles, which are perpendicular to each other, ensuring that the laser light entering the sample pool will be refracted in the pyramid space composed of the plane mirrors and emitted perpendicularly to the horizontal plane. The blue light of 405 nanometers is then filtered out by the filter in the sensing device, leaving the fluorescence reflecting the oil component, which is then displayed on the display screen of the central computer through the spectrometer.

[0021] 6. In the detection method of the present invention, the detection laser is accurately shot into the reaction device through primary reflection and secondary reflection, and then transmitted to the spectrometer for image analysis and processing, and then displayed on the display screen of the central computer. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Other features, objects and advantages of the present invention will become more apparent from the detailed description of non-limiting embodiments made with reference to the following drawings: Figure 1 It is a flow chart of the working method of the present invention; Figure 2 It is a schematic diagram of the overall structure of the present invention; Figure 3 It is a schematic diagram of the structure of the support device of the present invention; Figure 4 for Figure 3 Enlarged view of point A in the middle; Figure 5 This is a schematic diagram of the internal structure of the functional disk in the present invention; Figure 6 It is a schematic diagram of the side structure of the rotating wheel in the present invention; Figure 7 It is a front view of the second angle deflection device in the present invention; Figure 8is a top view of the second angle deflection device in the present invention; Fig. 9 It is a schematic diagram of the structure of the sensing device in the present invention. DETAILED DESCRIPTION

[0023] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further explained below in conjunction with specific implementation methods.

[0024] See also Figure 1-Figure 9 The present invention provides a marine pollutant identification device using laser induced fluorescence, comprising a laser emitter 1, a support device 2, a reflector 3 arranged on the support device 2, a sample pool 9, a sensing device 5, a spectrometer 6 and a central computer 7, wherein the laser emitter 1 emits laser light which enters the sample pool 9 after being refracted by the reflector 3, the support device 2 is provided with a first angle deflection device 4 for adjusting the deflection angle of the reflector 3, the sample pool 9 is provided with a second angle deflection device for further deflecting the laser light into the sensing device 5, the laser light filtered by the sensing device 5 is input into the spectrometer 6 through an optical fiber and the result is presented in the central computer 7, wherein the laser emitter 1 emits a 405 nm laser beam, the support device 2 comprises a support base 21, a support rod 22 fixedly connected to the support base 21, and a functional disk 23 fixedly arranged on the top of the support rod 22; The first angle deflection device 4 includes a first connecting rod 41, a second connecting rod 42, and a rotating wheel 43 movably connected to the first connecting rod 41 and the second connecting rod 42 respectively. A functional cavity 8 is provided in the functional disk 23. The first angle deflection device 4 is provided in the functional cavity 8. The first connecting rod 41 and the second connecting rod 42 are parallel to each other and perpendicular to the horizontal plane. A first protrusion 412 is fixedly provided on the first connecting rod 41, and a second protrusion 422 is fixedly provided on the second connecting rod 42. The functional cavity 8 is provided with a first elongated hole 231 and a second elongated hole 232, both of which penetrate to the outer surface of the functional disk 23, the first protrusion 412 extends out of the functional cavity 8 via the first elongated hole 231, and the second protrusion 422 extends out of the functional cavity 8 via the second elongated hole 232, and the two ends of the reflector 3 are respectively hinged to the first protrusion 412 and the second protrusion 422, and the reflector 3 is an assembled mirror, that is, two parts spliced ​​together.

[0025] The first connecting rod 41 is provided with a first meshing tooth 411, the second connecting rod 42 is provided with a second meshing tooth 421, and the outer circumferential side surface of the rotating wheel is provided with a third meshing tooth 431. The first connecting rod 41 and the second connecting rod 42 are arranged on both sides of the rotating wheel, and the third meshing tooth 431 is respectively meshed with the first meshing tooth 411 and the second meshing tooth 421. The rotating wheel includes a coaxially arranged rotating inner wheel 43 and a rotating outer wheel 44. The rotating inner wheel 43 is arranged in the functional cavity 8 and is connected with the first connecting rod 41 and the second connecting rod 42. The rotating outer wheel 44 extends outward to the outer surface of the functional disk 23. The rotating outer wheel 44 and the rotating inner wheel 43 are connected by an elastic telescopic rod 45. The rotating outer wheel 44 is driven to rotate by an external force, thereby driving the rotating inner wheel 43 to rotate.

[0026] Through the elastic action of the elastic telescopic rod 45, the rotating outer wheel 44 moves away from the rotating inner wheel 43 under the action of external force or approaches the rotating inner wheel 43 in a power-losing state. The outer surface of the functional disk 23 is provided with a first positioning latch tooth 441 around the rotating outer wheel 44, and the outer circumferential surface of the rotating outer wheel 44 is provided with a second positioning latch tooth 233. The first positioning latch tooth 441 and the second positioning latch tooth 233 are meshed with each other. When an external force is applied to the rotating outer wheel 44 to move it away from the rotating inner wheel 43, the first positioning latch tooth 441 and the second positioning latch tooth 233 are separated from each other. When the rotating outer wheel 44 loses power and approaches the rotating inner wheel 43, the first positioning latch tooth 441 and the second positioning latch tooth 233 are engaged with each other.

[0027] The second angle deflection device includes three plane mirrors 91 spliced ​​together, each of the plane mirrors 91 has the same specifications and is in the shape of an isosceles right triangle, and the right-angled sides of two adjacent plane mirrors 91 are connected to form a pyramid space, and the sensing device 5 includes a receiving probe 51 and a filter 52, which are used to receive the laser beam after passing through the second angle deflection device in the sample pool, wherein the filter 52 can filter out the laser with a wavelength of 405 nanometers, leaving only the fluorescence converted by the oil stain.

[0028] This embodiment simultaneously proposes a method of using the above invention, a working method of a marine pollutant identification device using laser induced fluorescence, comprising the following steps: S1, seawater sampling: taking a seawater sample from the seawater and placing it in a sample pool 9; S2, emitting laser; using a 405nm laser emitter for laser emission, the emitted laser beam enters the sample cell 9 after being reflected once by the reflector 3; S3, secondary reflection; the laser light entering the sample pool 9 passes through the second angle deflection device and then is emitted vertically upward to the sensing device 5; S4, laser filtering; the filtered laser is poured into the spectrometer 6 via the optical fiber and the data is then transmitted to the central computer 7; S5, data analysis; the central computer 7 analyzes and processes the data and presents it on the display screen.

[0029] The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention. It is obvious to those skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, and the present invention can be implemented in other specific forms without departing from the spirit or basic features of the present invention. Therefore, no matter from which point of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the present invention is defined by the attached claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims are included in the present invention. Any figure mark in the claims should not be regarded as limiting the claims involved.

[0030] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A marine pollutant identification device using laser induced fluorescence, characterized in that: The invention comprises a laser transmitter, a supporting device, a reflecting mirror arranged on the supporting device, a sample pool, a sensing device, a spectrometer and a central computer. The laser transmitter transmits laser light which enters the sample pool after being refracted by the reflecting mirror. The supporting device is provided with a first angle deflection device for adjusting the deflection angle of the reflecting mirror. The sample pool is provided with a second angle deflection device for further deflecting the laser light into the sensing device. The laser light filtered by the sensing device is input into the spectrometer through an optical fiber and the result is presented in the central computer.

2. The marine pollutant identification device using laser induced fluorescence according to claim 1 is characterized in that: The support device comprises a support base, a support rod fixedly connected to the support base, and a functional plate fixedly arranged on the top of the support rod.

3. The marine pollutant identification device using laser induced fluorescence according to claim 2 is characterized in that: The first angle deflection device comprises a first connecting rod, a second connecting rod, and a rotating wheel movably connected to the first connecting rod and the second connecting rod respectively. A functional cavity is arranged in the functional disk, and the first angle deflection device is arranged in the functional cavity.

4. The marine pollutant identification device using laser induced fluorescence according to claim 3 is characterized by: The first connecting rod and the second connecting rod are parallel to each other and perpendicular to the horizontal plane. The first connecting rod is fixedly provided with a first protrusion, and the second connecting rod is fixedly provided with a second protrusion. The functional cavity is provided with a first elongated hole and a second elongated hole. The first elongated hole and the second elongated hole both penetrate to the outer surface of the functional disk. The first protrusion extends out of the functional cavity via the first elongated hole, and the second protrusion extends out of the functional cavity via the second elongated hole. Both ends of the reflector are hinged to the first protrusion and the second protrusion respectively.

5. The marine pollutant identification device using laser induced fluorescence according to claim 4 is characterized in that: The first connecting rod is provided with a first meshing tooth, the second connecting rod is provided with a second meshing tooth, and the outer circumferential side surface of the rotating wheel is provided with a third meshing tooth. The first connecting rod and the second connecting rod are provided on both sides of the rotating wheel, and the third meshing tooth is respectively meshed with the first meshing tooth and the second meshing tooth.

6. The marine pollutant identification device using laser induced fluorescence according to claim 5, characterized in that: The rotating wheel includes a coaxially arranged rotating inner wheel and a rotating outer wheel, the rotating inner wheel is arranged in the functional cavity and connected to a first connecting rod and a second connecting rod, the rotating outer wheel extends outward to the outer surface of the functional disk, the rotating outer wheel and the rotating inner wheel are connected by an elastic telescopic rod, and the rotating outer wheel is driven to rotate by an external force, thereby driving the rotating inner wheel to rotate.

7. The marine pollutant identification device using laser induced fluorescence according to claim 6, characterized in that: Through the elastic action of the elastic telescopic rod, the rotating outer wheel moves away from the rotating inner wheel under the action of external force or approaches the rotating inner wheel in a power-losing state. The outer surface of the functional disk is provided with a first positioning tooth around the rotating outer wheel, and the outer circumferential surface of the rotating outer wheel is provided with a second positioning tooth. The first positioning tooth and the second positioning tooth are meshed with each other. When an external force is applied to the rotating outer wheel to move it away from the rotating inner wheel, the first positioning tooth and the second positioning tooth are separated from each other. When the rotating outer wheel loses power and approaches the rotating inner wheel, the first positioning tooth and the second positioning tooth are engaged with each other.

8. The marine pollutant identification device using laser induced fluorescence according to claim 7 is characterized in that: The second angle deflection device includes three plane mirrors spliced ​​together, each of the plane mirrors has the same specifications and is in the shape of an isosceles right triangle, and the right-angled sides of two adjacent plane mirrors are connected to form a pyramid space.

9. The marine pollutant identification device using laser induced fluorescence according to claim 8, characterized in that: The sensing device comprises a receiving probe and a filter, which are used to receive the laser beam after passing through the second angle deflection device in the sample cell.

10. A method for operating the marine pollutant identification device using laser induced fluorescence as claimed in claim 9, characterized in that: The following steps are involved: S1. Seawater sampling: sampling seawater from seawater and placing it in a sample pool; S2, emitting laser: using a 405nm laser emitter for laser emission, the emitted laser beam enters the sample pool after being reflected once by the reflector; S3, secondary reflection; the laser light entering the sample cell passes through the second angle deflection device and then is emitted vertically upward to the sensing device; S4, laser filtering; the filtered laser is poured into the spectrometer via optical fiber and the data is then transmitted to the central computer; S5. Data analysis: The central computer analyzes and processes the data and displays it on the display screen.

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