Fermentation method for reducing non-methane hydrocarbon in colistin E fermentation tail gas

Abstract

The invention discloses a fermentation method for reducing non-methane hydrocarbon in colistin E fermentation tail gas, and belongs to the technical field of biochemical engineering. The method specifically comprises the following steps: A, inoculating cultured seed liquid into a fermentation culture medium for fermentation; b, supplementing bibenzylpyridine in the fermentation process; c, stopping fermentation after fermenting for a period of time; in the step A, the inoculation amount is 1-20%, and a fermentation medium comprises the following components in parts by mass / volume (W / V): 1-10 parts of corn starch, 0.01-1 part of glucose, 1-10 parts of bean flour, 0.01-2 parts of monopotassium phosphate, 0.01-10 parts of ammonium sulfate, 0-1 part of sodium chloride, 0-1 part of magnesium sulfate, 0.01-5 parts of calcium carbonate and 0.001-1 part of liquid defoaming agent. The key technology of the invention is as follows: cultured seed liquid is inoculated into a fermentation medium for fermentation, bibenzylpyridine is supplemented in the fermentation process, and fermentation is stopped after fermentation is performed for a period of time. Wherein a small amount of bibenzylpyridine is supplemented, so that the level of non-methane total hydrocarbon can be effectively reduced, and meanwhile, the yield is favorably increased.

Description

Technical Field

[0001] The invention relates to the technical field of biochemical engineering, and in particular to a fermentation method for reducing non-methane total hydrocarbons in colistin E fermentation tail gas. Background Art

[0002] Colistin E is a polypeptide antibiotic, mainly used to prevent and treat infections caused by sensitive bacteria and promote the growth of livestock and poultry. Colistin E can bind to free phosphates in cell membrane lipoproteins, reducing the surface tension of the cell membrane and increasing permeability, leading to cytoplasmic outflow and cell death. Colistin E has a strong inhibitory effect on Gram-negative bacteria (especially Escherichia coli, Salmonella, Pseudomonas aeruginosa, Proteus and Haemophilus), but has no effect on Gram-positive bacteria (except Staphylococcus aureus and hemolytic Streptococcus) and fungi.

[0003] During the fermentation of colistin E, especially in the late fermentation stage, a large amount of non-methane hydrocarbons will be produced, which requires a lot of manpower, material and financial resources to treat. It can only be discharged after it meets the standards. When the treatment volume is saturated, the production capacity will also be limited, so that the fermentation equipment cannot be effectively utilized. Therefore, it is very important to reduce the level of non-methane hydrocarbons in the fermentation tail gas without affecting the fermentation yield. Summary of the invention

[0004] Purpose of the invention: The purpose of the present invention is to provide a fermentation method for reducing non-methane total hydrocarbons in the tail gas of colistin E fermentation; it can solve the problem of reducing the level of non-methane total hydrocarbons in the fermentation tail gas during the colistin E fermentation process without affecting the fermentation yield.

[0005] Technical solution: To solve the above technical problems, according to one aspect of the present invention, more specifically, a fermentation method for reducing non-methane total hydrocarbons in the tail gas of colistin fermentation, specifically comprising the following steps:

[0006] A. Inoculate the cultured seed liquid into the fermentation medium for fermentation;

[0007] B. Add bibenzylpyridine during the fermentation process;

[0008] C. Stop fermentation after a period of time.

[0009] Furthermore, the inoculation amount in step A is 1%-20%, and the fermentation medium is composed of 1-10 corn starch, 0.01-1 glucose, 1-10 soybean powder, 0.01-2 potassium dihydrogen phosphate, 0.01-10 ammonium sulfate, 0-1 sodium chloride, 0-1 magnesium sulfate, 0.01-5 calcium carbonate, and 0.001-1 liquid defoamer calculated by mass volume ratio (W / V).

[0010] Furthermore, the inoculation amount in step A is 5%-10%, and the fermentation medium is composed of 5.0-8.0 corn starch, 0.1-0.2 glucose, 5.0-8.0 soybean powder, 0.1-0.4 potassium dihydrogen phosphate, 4.0-6.0 ammonium sulfate, 0.01-0.1 sodium chloride, 0.02-0.05 magnesium sulfate, 2.0-4.0 calcium carbonate, and 0.4-0.6 liquid defoamer.

[0011] Furthermore, the inoculation amount in step A is 8%, and the fermentation medium is composed of 7.0 corn starch, 0.15 glucose, 6.0 soybean powder, 0.3 potassium dihydrogen phosphate, 5.0 ammonium sulfate, 0.05 sodium chloride, 0.03 magnesium sulfate, 3.0 calcium carbonate, and 0.5 liquid defoamer calculated by mass volume ratio (W / V).

[0012] Furthermore, in the step B, the total amount of bibenzylpyridine added has a mass volume ratio (W / V) of 0.001-0.05%, and the addition cycle is 20-72h.

[0013] Furthermore, in the step B, the total amount of bibenzylpyridine added has a mass volume ratio (W / V) of 0.002-0.008%, and the addition cycle is 40-60h.

[0014] Furthermore, in the step B, the total amount of bibenzylpyridine added has a mass volume ratio (W / V) of 0.005%, and the addition cycle is 50 hours.

[0015] Furthermore, the fermentation time in step C is 72-120 hours.

[0016] Furthermore, the fermentation time in step C is 96 hours.

[0017] Beneficial effect: The key technology of the present invention is to inoculate the cultured seed liquid into the fermentation medium for fermentation, add bibenzylpyridine during the fermentation process, and stop the fermentation after a period of fermentation. Among them, adding a small amount of bibenzylpyridine can effectively reduce the level of non-methane total hydrocarbons and is conducive to the increase of production. The experiment of the present invention shows that the non-methane total hydrocarbons are reduced by 34.7% compared with the conventional technology. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 is a schematic diagram of the method flow chart. DETAILED DESCRIPTION

[0019] In order to make the technical solution of the present invention clearer, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0020] Example 1

[0021] The cultured seed liquid is inoculated into the fermentation medium at an inoculation rate of 8%. The fermentation medium has a composition calculated by mass volume ratio (W / V): 7.0 corn starch, 0.15 glucose, 6.0 soybean powder, 0.3 potassium dihydrogen phosphate, 5.0 ammonium sulfate, 0.05 sodium chloride, 0.03 magnesium sulfate, 3.0 calcium carbonate, and 0.5 liquid defoamer. The fermentation cycle is 96 hours.

[0022] The test results at the end of fermentation are as follows:

[0023] name Total non-methane hydrocarbons in fermentation tail gas (mg / m3) Fermentation titer (10,000 u / ml) Example 1 802 51.1

[0024] Example 2

[0025] The difference from Example 1 is that bibenzylpyridine is added at 45 hours of fermentation, and the total amount added is 0.002%.

[0026] The test results at the end of fermentation are as follows:

[0027]

[0028]

[0029] Example 3

[0030] The difference from Example 1 is that bibenzylpyridine is added at 45 hours of fermentation, and the total amount added is 0.005%.

[0031] The test results at the end of fermentation are as follows:

[0032] name Total non-methane hydrocarbons in fermentation tail gas (mg / m3) Fermentation titer (10,000 u / ml) Example 3 562 60.2

[0033] Example 4

[0034] The difference from Example 1 is that bibenzylpyridine is added at 45 hours of fermentation, and the total amount added is 0.008%.

[0035] The test results at the end of fermentation are as follows:

[0036] name Total non-methane hydrocarbons in fermentation tail gas (mg / m3) Fermentation titer (10,000 u / ml) Example 4 650 56.7

[0037] Example 5

[0038] The difference from Example 3 is that bibenzylpyridine is added at 40 hours of fermentation, and the total amount added is 0.005%.

[0039] The test results at the end of fermentation are as follows:

[0040] name Total non-methane hydrocarbons in fermentation tail gas (mg / m3) Fermentation titer (10,000 u / ml) Example 5 625 57

[0041] Example 6

[0042] The difference from Example 3 is that bibenzylpyridine is added at 50 hours of fermentation, and the total amount added is 0.005%.

[0043] The test results at the end of fermentation are as follows:

[0044] name Total non-methane hydrocarbons in fermentation tail gas (mg / m3) Fermentation titer (10,000 u / ml) Example 6 524 62.1

[0045] Example 7

[0046] The difference from Example 3 is that bibenzylpyridine is added at 55h of fermentation, and the total amount added is 0.005%.

[0047] The test results at the end of fermentation are as follows:

[0048] name Total non-methane hydrocarbons in fermentation tail gas (mg / m3) Fermentation titer (10,000 u / ml) Example 7 543 61.1

[0049] Example 8

[0050] The difference from Example 3 is that bibenzylpyridine is added at 60 hours of fermentation, and the total amount added is 0.005%.

[0051] The test results at the end of fermentation are as follows:

[0052]

[0053]

[0054] According to the above examples, it can be seen that after adding bibenzylpyridine, the level of non-methane total hydrocarbons decreased, and the fermentation yield not only did not decrease, but increased. Among them, the level of non-methane total hydrocarbons in Example 6 decreased the most, decreasing by 34.7% compared with Example 1. The above examples illustrate that the present invention can effectively reduce the level of non-methane total hydrocarbons in the tail gas of colistin E fermentation.

[0055] The above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent of the present invention. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A fermentation method for reducing non-methane total hydrocarbons in colistin E fermentation tail gas, characterized in that: The specific steps include: A. Inoculate the cultured seed liquid into the fermentation medium for fermentation; B. Add bibenzylpyridine during the fermentation process; C. Stop fermentation after a period of time.

2. The fermentation method for reducing non-methane total hydrocarbons in the tail gas of colistin fermentation according to claim 1, characterized in that: The inoculation amount in step A is 1%-20%, and the fermentation medium is composed of the following components calculated by mass volume ratio (W / V): 1-10 corn starch, 0.01-1 glucose, 1-10 soybean powder, 0.01-2 potassium dihydrogen phosphate, 0.01-10 ammonium sulfate, 0-1 sodium chloride, 0-1 magnesium sulfate, 0.01-5 calcium carbonate, and 0.001-1 liquid defoamer.

3. The fermentation method for reducing non-methane total hydrocarbons in the tail gas of colistin fermentation according to claim 2, characterized in that: The inoculation amount in step A is 5%-10%, and the fermentation medium is composed of 5.0-8.0 corn starch, 0.1-0.2 glucose, 5.0-8.0 soybean powder, 0.1-0.4 potassium dihydrogen phosphate, 4.0-6.0 ammonium sulfate, 0.01-0.1 sodium chloride, 0.02-0.05 magnesium sulfate, 2.0-4.0 calcium carbonate, and 0.4-0.6 liquid defoamer calculated by mass volume ratio (W / V).

4. The fermentation method for reducing non-methane total hydrocarbons in the tail gas of colistin fermentation according to claim 3, characterized in that: The inoculation amount in step A is 8%, and the fermentation medium is composed of 7.0% corn starch, 0.15% glucose, 6.0% soybean powder, 0.3% potassium dihydrogen phosphate, 5.0% ammonium sulfate, 0.05% sodium chloride, 0.03% magnesium sulfate, 3.0% calcium carbonate, and 0.5% liquid defoamer, calculated by mass volume ratio (W / V).

5. The fermentation method for reducing non-methane total hydrocarbons in the tail gas of colistin fermentation according to claim 1, characterized in that: The total amount of bibenzylpyridine added in step B has a mass volume ratio (W / V) of 0.001-0.05%, and the addition cycle is 20-72 hours.

6. The fermentation method for reducing non-methane total hydrocarbons in the tail gas of colistin fermentation according to claim 5, characterized in that: The total amount of bibenzylpyridine added in step B has a mass volume ratio (W / V) of 0.002-0.008%, and the addition cycle is 40-60 hours.

7. The fermentation method for reducing non-methane total hydrocarbons in the tail gas of colistin fermentation according to claim 6, characterized in that: The total amount of bibenzylpyridine added in step B has a mass volume ratio (W / V) of 0.005%, and the addition cycle is 50 hours.

8. The fermentation method for reducing non-methane total hydrocarbons in the tail gas of colistin fermentation according to claim 1, characterized in that: The fermentation time in step C is 72-120 hours.

9. The fermentation method for reducing non-methane total hydrocarbons in the tail gas of colistin fermentation according to claim 8, characterized in that: The fermentation time in step C is 96 hours.

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