Composition with functions of improving mitochondrial function and protecting microsphere structure as well as preparation method and application of composition

Abstract

The invention discloses a composition with functions of improving a mitochondrial function and protecting a microsphere structure as well as a preparation method and application of the composition. The composition comprises the following components in parts by weight: 1-10 parts of microspheroidized PDRN, 8-15 parts of other anti-aging active components, 1-5 parts of a preservative, 5-10 parts of a humectant, 0.1-1 part of a thickening agent and the balance of water, and the sum of the parts by weight of the components is 100 parts. According to the compound composition disclosed by the invention, the stability of the microspheroidized PDRN subjected to microspheroidizing treatment in formula application is improved. The anti-aging effect of the microspheroidized PDRN and other compositions on mitochondria is exerted to the greatest extent. On one hand, the spatial stability of the microsphere structure in the composition is protected; on the other hand, the release rate of the microspheres is adjusted, so that active ingredients in cosmetics can be slowly and continuously released, the action effect of the active ingredients is improved, in addition, multiple-effect raw materials are compounded, and the aim of precise mitochondrial targeting anti-aging is achieved through cooperation of direct and indirect effects from multiple dimensions.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and particularly relates to a composition having the functions of improving mitochondrial function and protecting microsphere structure, and a preparation method and application thereof. Background Art

[0002] With the acceleration of the modern life rhythm and the increase of environmental pressure, the problem of skin aging has attracted increasing attention. Mitochondria, as the "energy factory" within cells, its function is closely related to skin health. Therefore, developing a composition with stable active ingredient efficacy and capable of effectively improving mitochondrial function and delaying skin aging has important market value and social significance.

[0003] PDRN is a polynucleotide with a molecular weight range of 50 to 1500 kDa. It exists in various organisms and is currently mainly extracted and purified from the sperm cells of salmon or chum salmon. Research shows that the DNA base composition of salmon has a similarity of up to 98% with the human DNA base composition, with extremely low rejection and the ability to repair damaged mitochondria and restore their normal functions, thereby ensuring that cells have sufficient energy for various life activities and stimulating cell youth and vitality. Based on these unique effects of PDRN, we apply it to the development of other anti-aging active ingredients.

[0004] However, during application, due to its high molecular weight and hydrophilicity, PDRN is difficult to be directly absorbed by the skin; and currently, during the delivery process of PDRN, the efficiency is low; it is easily degraded by nucleases and inactivated; it is difficult to be formulated in a formula. Aiming at the pain points of PDRN, CN202310633541.7 discloses a preparation method of microsphere PDRN to solve the problems of transdermal penetration and degradation of PDRN. This composition uses this raw material to repair mitochondrial dysfunction caused by DNA damage. However, the stability of the microsphere structure formed by the self-assembly of this PDRN during formula application needs to be studied. (1) The properties and concentrations of other additives in the formula may affect the structural stability of microsphere PDRN and cause its microspheres to be damaged; (2) The formula production process conditions will also have a certain impact on the stability of microsphere PDRN; (3) After the structure of microsphere PDRN is damaged, the active ingredients encapsulated therein may be affected in terms of release, stability, and activity, reducing the anti-aging ability of the active ingredients themselves against mitochondria. Summary of the Invention

[0005] The purpose of the present invention is to solve the problems that the efficacy of products for improving mitochondrial function in the prior art is not significant and the microsphere PDRN is easily damaged during actual application, and to provide a composition having the functions of improving mitochondrial function and protecting microsphere structure, and a preparation method and application thereof.

[0006] In a first aspect, the present invention provides a composition having the functions of improving mitochondrial function and protecting microsphere structure, and the composition comprises the following components in parts by weight: microsphereized PDRN 1 to 10 parts, other anti-aging active ingredients 8 to 15 parts, preservatives 1 to 5 parts, humectants 5 to 10 parts, thickeners 0.1 to 1 part, and the balance is water, and the sum of the parts by weight of the above components is 100 parts.

[0007] Further, the preservative is at least one of polyol preservatives, alcohol preservatives, and parabens;

[0008] Among them, the polyol preservatives are at least one of 1,3-butanediol, 1,2-pentanediol, and 1,2-hexanediol;

[0009] Among them, the alcohol preservative is phenoxyethanol;

[0010] Among them, the parabens are at least one of methyl paraben and ethyl paraben.

[0011] Further, the thickener is at least one of cellulose thickeners and polysaccharide thickeners;

[0012] Among them, the cellulose thickeners are at least one of cellulose, cellulose gum, methyl cellulose, hydroxyethyl cellulose, and ethyl hydroxyethyl cellulose;

[0013] Among them, the polysaccharide thickeners are at least one of alginate, agarose, pectin, and extracellular polysaccharide.

[0014] Further, the humectant is at least two of polysaccharide humectants, polyether polyol humectants, and fatty acid humectants;

[0015] Among them, the polysaccharide humectants are at least one of hydrolyzed scleroglucan, β-glucan, hyaluronic acid, trehalose, biosaccharide gum, fucoidan, alginate, mannose, and erythritol;

[0016] Among them, the polyether polyol humectants are at least one of glycerol polyether-7 and glycerol polyether-26;

[0017] Among them, the fatty acid humectant is sodium alginate.

[0018] Further, the other anti-aging active ingredients include: anti-inflammatory agents, whitening agents, antioxidants, and mitochondrial energy supplements.

[0019] Among them, the anti-inflammatory agents are at least one of centella asiatica extract, portulaca oleracea extract, palmitoyl tetrapeptide-7, zinc hyaluronate, and aloe vera extract.

[0020] Among them, the whitening agent is at least one of glabridin, milk extract, daisy extract, azelaic acid, niacinamide, tranexamic acid, and ascorbyl glucoside.

[0021] Among them, the mitochondrial energy supplement is at least one of decapeptide-1, coenzyme Q10, α-lipoic acid, and nicotinamide adenine dinucleotide.

[0022] In a second aspect, the present invention provides a method for preparing the above-mentioned composition having the functions of improving mitochondrial function and protecting microsphere structure, comprising the following steps:

[0023] Mix a thickening agent with a polyether polyol humectant to obtain a mixture;

[0024] After swelling the mixture, perform stirring treatment and temperature recovery treatment in sequence to obtain a gel solution after temperature recovery;

[0025] Add an aqueous solution of a polysaccharide humectant to the gel solution after temperature recovery to obtain a first mixed gel solution;

[0026] When the temperature of the first mixed gel solution naturally drops to 60 °C, add a preservative to obtain a second mixed gel solution; when the temperature of the second mixed gel solution naturally drops to 40 °C, add an aqueous solution of microsphere PDRN and an aqueous solution of other anti-aging active ingredients to obtain a third mixed gel solution;

[0027] When the temperature of the third mixed gel solution naturally drops to 30 °C and the pH value is detected to be 5.5 - 6.5, the composition having the functions of improving mitochondrial function and protecting microsphere structure is obtained.

[0028] In a third aspect, the present invention provides an application of the above-mentioned composition having the functions of improving mitochondrial function and protecting microsphere structure in the preparation of anti-aging skin care products.

[0029] The beneficial effects of the present invention are as follows:

[0030] 1) The stability of microsphere PDRN after microsphere treatment in formula application is improved. The anti-aging effects of microsphere PDRN and other compositions on mitochondria are maximally exerted. On the one hand, the spatial stability of the microsphere structure in the combined components is protected; on the other hand, the release rate of the microspheres is regulated, enabling the active ingredients in the cosmetics to be slowly and continuously released, thereby improving their efficacy.

[0031] 2) Multiple efficacy raw materials are compounded, and the purpose of precise mitochondrial targeting anti-aging is achieved through the synergy of direct and indirect effects from multiple dimensions.

[0032] 3) The polyol adheres to the surface of the microspheres through physical adsorption, and a protective layer can be formed on the surface of the microspheres. This adsorption effect can effectively isolate harmful substances such as excess moisture, oxygen, and microorganisms in the external environment to a certain extent, thereby slowing down the erosion rate of the microsphere surface, preventing adverse reactions such as deformation and cracking due to changes in the external environment, and preventing the microspheres from being damaged.

[0033] 4) The modified side chains of the cellulose thickener form a three-dimensional network structure through the entanglement of molecular chains and the interweaving of molecules. The medium and solute are surrounded in the mesh and cannot flow freely. This thickening effect protects the microsphere structure to a certain extent and prevents the excessive aggregation or sedimentation of the microspheres in the fluid. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] The present invention will be further described below in conjunction with the drawings.

[0035] FIG. 1 is a microsphere structure diagram observed under a transmission electron microscope of a composition prepared in Example 1 of the present invention, which has the functions of improving mitochondrial function and protecting the microsphere structure. Figure 1a It is an enlarged x50.0k image; Figure 1b It is an enlarged x120.0k image; A large number of microsphere particles with regular shapes, uniform sizes, and clear boundaries and a particle size of about 120 nm can be clearly observed.

[0036] Figure 2 FIG. is a structure diagram observed under a transmission electron microscope of the composition prepared in Comparative Example 1. It is an enlarged x10.0k image. A large number of circular particles with a particle size of about 200 - 500 nm can be observed, which are not PDRN microsphere particles.

[0037] Figure 3 FIG. is a structure diagram observed under a transmission electron microscope of the composition prepared in Comparative Example 2. It is an enlarged x60.0k image. Microsphere particles with a particle size of about 120 nm can also be observed, but the edge clarity has decreased, and the integrity and structural fullness of the microspheres have also decreased.

[0038] Figure 4 FIG. is a structure diagram observed under a transmission electron microscope of the composition prepared in Comparative Example 3. It is an enlarged x3.0k image. A large number of sheet-like structures and circular particles with a particle size of about 400 - 600 nm can be observed, which are not microsphere particles.

[0039] Figure 5 FIG. is a structure diagram observed under a transmission electron microscope of the composition prepared in Comparative Example 4. It is an enlarged x50.0k image. Microsphere particles with a particle size of about 120 nm can also be observed, but the edge clarity has decreased, and the integrity and structural fullness of the microspheres have also decreased.

[0040] Figure 6 To verify the mitochondrial permeability transition pore (mPTP) fluorescence images of each group in the experiment.

[0041] Figure 7 Bar chart of the average fluorescence intensity of each group in the mitochondrial permeability transition pore (mPTP) test in the verification experiment. Note: * represents the significance analysis between the "model group" and the "control group", * indicates P-value < 0.05, ** indicates P-value < 0.01, *** indicates P-value < 0.001. # represents the significance analysis between the "experimental group" and the "model group", # indicates P-value < 0.05, ## indicates P-value < 0.01, indicates P-value < 0.001. Specific implementation manner

[0042] To better understand the present invention, the following describes the preferred specific embodiments of the present invention in detail to explain and illustrate the present invention, but does not limit the present invention in any form.

[0043] The raw materials or chemical reagents used in the embodiments of the present invention are obtained through conventional commercial channels unless otherwise specified.

[0044] Raw materials: Microsphere PDRN is purchased from Polyglycoside Tai® Microsphere PDRN of Shaanxi Baihong Synthetic Biology Technology Co., Ltd.

[0045] Example 1

[0046] This example provides a composition having the functions of improving mitochondrial function and protecting the microsphere structure. By weight, it includes the following raw material components:

[0047] 1 g of microsphere PDRN; 8.6 g of other anti-aging active ingredients: 0.1 g of Centella asiatica extract (anti-inflammatory agent), 2 g of milk extract (whitening agent), 4 g of tetrahydrocurcumin (antioxidant), 2.5 g of decapeptide-1 (mitochondrial energy supplement); 3.2 g of polyol preservatives: 1.2 g of 1,2-hexanediol and 2 g of 1,2-pentanediol; polysaccharide humectant: 0.5 g of hydrolyzed sclerotium gum; polyether polyol humectant: 5 g of glycerol polyether-26; cellulose thickener: 0.3 g of hydroxyethyl cellulose; 81.4 mL of water.

[0048] This example also provides a preparation method of a composition having the functions of improving mitochondrial function and protecting the microsphere structure, including the following steps:

[0049] Step 1: Pre-dissolution of active substances: Weigh 1 g of microsphere PDRN and add it to 3 mL of water, dissolve it by manual stirring for standby; weigh 0.1 g of Centella asiatica extract and add it to 1 mL of water, dissolve it by manual stirring for standby; weigh 2 g of milk extract and add it to 3 mL of water, dissolve it by manual stirring for standby; weigh 4 g of tetrahydrocurcumin and add it to 5 mL of water, dissolve it by manual stirring for standby; weigh 2.5 g of decapeptide-1 and add it to 3 mL of water, dissolve it by manual stirring for standby;

[0050] Step 2: Weigh 0.3 g of hydroxyethyl cellulose and add it to the reaction container, then add 5 g of glycerol polyether-26 for pre-dispersion, stir for 3 min until fully mixed to obtain a mixture;

[0051] Step 3: Add 60 mL of water to the mixture in Step 2, then place it in a water bath at 85 °C and swell it fully for 30 min, then transfer it to a stirrer and stir at a speed of 1000 rpm for 10 min until the hydroxyethyl cellulose is completely dissolved to obtain a transparent gel solution;

[0052] Step 4: Place the transparent gel solution in Step 3 in a water bath at 85 °C for 20 min for temperature recovery treatment to obtain a temperature-recovered gel solution;

[0053] Step 5: Weigh 0.5 g of hydrolyzed sclerotium gum and add it to 3 mL of water, dissolve it by manual stirring, and after complete dissolution, add it to the temperature-recovered gel solution in Step 4 to obtain a first mixed gel solution;

[0054] Step 6: Use a stirrer to stir the first mixed gel solution in Step 5 at a speed of 1000 rpm. When the temperature of the first mixed gel solution naturally drops to 60 °C, add 1.2 g of 1,2-hexanediol and 2 g of 1,2-pentanediol respectively to obtain a second mixed gel solution, and continue to stir at a speed of 1000 rpm; when the temperature of the second mixed gel solution naturally drops to 40 °C, add the pre-dissolved active substance components in Step 1 (the microsphere PDRN solution, Centella asiatica extract solution, milk extract solution, tetrahydrocurcumin solution and decapeptide-1 solution in Step 1), stir at a speed of 1500 rpm for 30 min, then add 3.4 mL of water, and then stir at a speed of 1000 rpm for 5 min to obtain a third mixed gel solution;

[0055] Step 7: Finally, when the temperature of the third mixed gel solution in Step 6 naturally drops to 30 °C, stop stirring, and the measured pH value is 5.8 to obtain a composition with the functions of improving mitochondrial function and protecting the microsphere structure.

[0056] Example 2

[0057] This example provides a composition that can improve mitochondrial function and protect the microsphere structure. By weight, it includes the following raw material components:

[0058] Microsphere PDRN 5 g; Other anti-aging active ingredients 11.3 g: respectively, Portulaca oleracea extract (anti-inflammatory agent) 1.1 g, glabridin (whitening agent) 2 g, tetrahydrocurcumin (antioxidant) 5 g, decapeptide-1 (mitochondrial energy supplement) 3.2 g; Polyol preservatives: 1,2-hexanediol 1.3 g, 1,2-pentanediol 2.2 g; Polysaccharide humectants: β-glucan 0.8 g; Polyether polyol humectants: 6.2 g of glycerol polyether-26, Cellulose thickeners: hydroxypropyl cellulose 0.6 g; The balance is water.

[0059] This example also provides a preparation method for a composition that can improve mitochondrial function and protect the microsphere structure, which is the same as that in Example 1.

[0060] Example 3

[0061] This example provides a composition that can improve mitochondrial function and protect the microsphere structure. By weight, it includes the following raw material components:

[0062] Microsphere PDRN 10 g; Other anti-aging active ingredients 15 g: respectively, palmitoyl tetrapeptide-7 (anti-inflammatory agent) 0.5 g, milk extract (whitening agent) 3.5 g, tetrahydrocurcumin (antioxidant) 6 g, decapeptide-1 (mitochondrial energy supplement) 5 g; Polyol preservatives: 1,2-hexanediol 2.2 g, 1,2-pentanediol 2 g; Polysaccharide humectants: trehalose 5 g; Polyether polyol humectants: glycerol polyether-26 5 g, Cellulose thickeners: hydroxyethyl cellulose 1 g; The balance is water.

[0063] This example also provides a preparation method for a composition that can improve mitochondrial function and protect the microsphere structure, which is the same as that in Example 1.

[0064] Comparative Example 1

[0065] This comparative example provides a composition. By weight, it includes the following raw material components:

[0066] Microsphere PDRN 1 g; Other anti-aging active ingredients 8.6 g: respectively, Centella asiatica extract (anti-inflammatory agent) 0.1 g, milk extract (whitening agent) 2 g, tetrahydrocurcumin (antioxidant) 4 g, decapeptide-1 (mitochondrial energy supplement) 2.5 g; Preservative: p-hydroxyacetophenone 3.2 g; Polysaccharide humectants: hydrolyzed sclerotium gum 0.5 g; Polyether polyol humectants: 5 g of glycerol polyether-26, Cellulose thickeners: hydroxyethyl cellulose 0.3 g; The balance is water.

[0067] Preparation method of the composition in this comparative example: Compared with Example 1, the preservative in this comparative example is p - hydroxyacetophenone, and the rest is the same as in Example 1.

[0068] Advantages of Example 1: The preservatives 1,2 - pentanediol and 1,2 - hexanediol have an obvious protective effect on the microsphere structure compared with p - hydroxyacetophenone. See Figure 2 The comparison with Figure 1. The polyhydric alcohol attaches to the surface of the microsphere by physical adsorption and can form a protective layer on the surface of the microsphere. This adsorption effect can effectively isolate harmful substances such as excess water, oxygen, and microorganisms in the external environment to a certain extent, thereby slowing down the erosion rate on the surface of the microsphere, preventing adverse reactions such as deformation and cracking due to changes in the external environment, and preventing the microsphere from being damaged.

[0069] Comparative Example 2

[0070] This comparative example provides a composition, which includes the following raw material components by weight:

[0071] Micro - spherized PDRN 1 g; other anti - aging active ingredients 8.6 g: Centella asiatica extract (anti - inflammatory agent) 0.1 g, milk extract (whitening agent) 2 g, tetrahydrocurcumin (antioxidant) 4 g, decapeptide - 1 (mitochondrial energy supplement) 2.5 g; polyhydric alcohol preservatives: 1,2 - hexanediol 1.2 g, 1,2 - pentanediol 2 g; fatty acid humectant: hydrolyzed sclerotium gum 0.5 g; polyether polyol humectant: 5 g of glycerol polyether - 26, xanthan gum - type thickener: xanthan gum 0.3 g; the balance is water.

[0072] Preparation method of the composition in this comparative example: Compared with Example 1, the thickener in this comparative example is xanthan gum, and the rest is the same as in Example 1.

[0073] Xanthan gum is an anionic thickener. The microspheres themselves are not resistant to ions. Their layer - by - layer assembly relies on van der Waals forces. Anionic thickening generates electrostatic interference on it, changing the charge distribution of surrounding molecules, thereby affecting the van der Waals forces between molecules and having a certain destructive effect on the stability of the microspheres.

[0074] Advantages of Example 1: The thickener being hydroxyethyl cellulose has an obvious protective effect on the microsphere structure compared with xanthan gum - type thickening. See Figure 3 The comparison with Figure 1. The modified side chains of the cellulose - type thickener are intertwined through molecular chains, and the molecules are interwoven with each other to form a three - dimensional network structure. The medium and solute are surrounded in the mesh holes and cannot flow freely. This thickening effect protects the microsphere structure to a certain extent and prevents excessive aggregation or sedimentation of the microspheres in the fluid.

[0075] Comparative Example 3

[0076] This comparative example provides a composition, which comprises the following raw material components by weight:

[0077] 1g of microspheroidized PDRN; 8.6g of other anti-aging active ingredients: 0.1g of Centella asiatica extract (anti-inflammatory agent), 2g of milk extract (whitening agent), 4g of tetrahydrocurcumin (antioxidant), 2.5g of decapeptide-1 (mitochondrial energy supplement); polyol preservatives: 1,2-hexanediol 1.2g, 1,2-pentanediol 2g; fatty acid moisturizer: 0.5g of hydrolyzed sclerotium gum; polyether polyol moisturizer: 5g of glyceryl polyether-26, carbomer thickener: 0.3g of carbomer 341; the balance is water.

[0078] Preparation method of the composition in this comparative example: Compared with Example 1, the thickener in this comparative example is Carbopol 341, and the rest is the same as Example 1.

[0079] Carbohydrate thickening is through neutralization thickening and hydrogen bonding thickening, which depends on alkaline neutralization and intermolecular interactions. The technical principle of microsphere raw materials is also to adjust pH and other methods to form non-covalent bonds (including hydrogen bonds). The two together will destroy each other, so carbohydrate thickening will destroy the microsphere structure to a certain extent.

[0080] Advantages of Example 1: The thickener is hydroxyethyl cellulose, which has a significant protective effect on the microsphere structure compared to carbomer thickeners. Figure 4 Compare with Figure 1. The reason is the same as stated in Comparative Example 2.

[0081] Comparative Example 4

[0082] This comparative example provides a composition, which comprises the following raw material components by weight:

[0083] Microspheroidized PDRN 1g; other anti-aging active ingredients 8.6g: Centella asiatica extract (anti-inflammatory agent) 0.1g, milk extract (whitening agent) 2g, tetrahydrocurcumin (antioxidant) 4g, decapeptide-1 (mitochondrial energy supplement) 2.5g; polyol preservatives: 1,2-hexanediol 1.2g, 1,2-pentanediol 2g; amino acid moisturizer: betaine 0.5g; fatty alcohol moisturizer: glycerin 5g, cellulose thickener: hydroxyethyl cellulose 0.3g; the balance is water.

[0084] Preparation method of the composition in this comparative example: Compared with Example 1, the moisturizing agent in this comparative example is an amino acid moisturizing agent: betaine; the fatty alcohol moisturizing agent: glycerin, and the rest is the same as Example 1.

[0085] In Example 1, the moisturizer is hydrolyzed sclerotium gum and glycerol polyether-26, which has a significant protective effect on the microsphere structure. Figure 5 Compare with Figure 1.

[0086] Comparative Example 5

[0087] This comparative example provides a composition, which includes the following raw material components by weight:

[0088] Microsphere PDRN 1 g; polyol preservatives: 1,2 - hexanediol 1.2 g, 1,2 - pentanediol 2 g; polysaccharide humectants: hydrolyzed sclerotium gum 0.5 g; polyether polyol humectants: 5 g of glycerol polyether - 26, cellulose thickeners: hydroxyethyl cellulose 0.3 g; the balance is water.

[0089] The preparation method of the composition in this comparative example: Compared with Example 1, no other anti - aging active ingredients are added in this comparative example, and the rest is the same as Example 1.

[0090] Mitochondrial anti - aging efficacy detection:

[0091] Mitochondrial permeability transition pore (mPTP) test:

[0092] Compare the changes in mitochondrial permeability transition pore (mPTP) after the actions of Example 1 and Comparative Example 5. The test method is as follows:

[0093] (a) Cell resuscitation and sub - culture: Take out the HFB cells at passage number P6 from the liquid nitrogen tank, resuscitate and inoculate them into a culture flask, culture them in an incubator at 37 °C and 5% CO2. When the cells grow to 70% - 80%, digest and sub - culture them. The digested cells are resuspended with the culture medium and counted for standby.

[0094] (b) Cell seeding: Seed the cells at an inoculation amount of 6×104 cells / well into a 12 - well plate, incubate overnight in an incubator (37 °C, 5% CO2), and the inoculation amount per well is 1 mL.

[0095] (c) Experimental grouping: When the cell confluence rate reaches 70% - 80%, take out the culture plate and set up a control group, a model group, and a sample group respectively.

[0096] (d) Solution preparation: Prepare the sample working solution according to the experimental plan table (Table 1).

[0097] (e) mPTP staining: After culturing the cells in the incubator for 24 h, discard the supernatant, add 1 mL of PBS to each well for washing, and then perform staining treatment according to the mPTP kit instructions.

[0098] (f) Photography: After staining, discard the supernatant, add 1 mL of PBS to each well, take pictures under a 10 - fold objective of a fluorescence inverted microscope, and record the fluorescence pictures of mPTP in each group, as shown in Figure 6 .

[0099] (g) Calculation of mPTP fluorescence intensity: For each group of samples, the average fluorescence intensity of each photo was statistically analyzed, and the SD value was calculated. The t-test method was used for statistical analysis to calculate the P value, as shown in Table 2.

[0100] Table 1 Experimental schedule

[0101]

[0102] Table 2 Summary table of mPTP results

[0103]

[0104] The results showed that: The average fluorescence intensity of mPTP in the cells of the Example 1 group (compound of multiple anti-aging active substances) with 2.5 μg / mL (m / v) of mitochondrial permeability transition pore was significantly increased compared with that of the Comparative Example 5 group (only containing microspherized PDRN); the increase rate was 17.50%, and the anti-aging effect of mitochondria was significantly more obvious, as shown in Figure 7 .

[0105] Although the preferred embodiments of the present invention have been described, those skilled in the art can make additional changes and modifications to these embodiments once they know the basic creative concept.

[0106] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and its equivalent technologies, the present invention is also intended to include these modifications and variations.

Claims

1. A composition having the effects of improving mitochondrial function and protecting microsphere structure, characterized in that: The composition comprises the following components in parts by weight: 1 to 10 parts of microspheroidized PDRN, 8 to 15 parts of other anti-aging active ingredients, 1 to 5 parts of preservatives, 5 to 10 parts of moisturizers, 0.1 to 1 part of thickeners, and the balance is water, and the sum of the parts by weight of the above components is 100 parts.

2. The composition having the effects of improving mitochondrial function and protecting microsphere structure according to claim 1, characterized in that: The preservative is at least one of a polyol preservative, an alcohol preservative, and a paraben preservative; The polyol preservative is at least one of 1,3-butanediol, 1,2-pentanediol, and 1,2-hexanediol; The alcohol preservative is phenoxyethanol; The paraben preservative is at least one of methyl paraben and ethyl paraben.

3. The composition having the effects of improving mitochondrial function and protecting microsphere structure according to claim 1, characterized in that: The thickener is at least one of a cellulose thickener and a polysaccharide thickener; The cellulosic thickener is at least one of cellulose, cellulose gum, methyl cellulose, hydroxyethyl cellulose, and ethyl hydroxyethyl cellulose; The polysaccharide thickener is at least one of alginate, agarose, pectin and exopolysaccharide.

4. The composition having the effects of improving mitochondrial function and protecting microsphere structure according to claim 1, characterized in that: The moisturizing agent is at least two of a polysaccharide moisturizing agent, a polyether polyol moisturizing agent, and a fatty acid moisturizing agent; The polysaccharide moisturizer is at least one of hydrolyzed sclerotium gum, β-glucan, hyaluronic acid, trehalose, biosaccharide gum, alginate, fucoidan, mannose, and erythritol; The polyether polyol moisturizer is at least one of glycerol polyether-7 and glycerol polyether-26; The fatty acid moisturizing agent is sodium alginate.

5. The composition having the effects of improving mitochondrial function and protecting microsphere structure according to claim 1, characterized in that: The other anti-aging active ingredients include: anti-inflammatory agents, whitening agents, antioxidants, and mitochondrial energy supplements.

6. The composition having the effects of improving mitochondrial function and protecting microsphere structure according to claim 5, characterized in that: The anti-inflammatory agent is at least one of Centella asiatica extract, Portulaca oleracea extract, Palmitoyl tetrapeptide-7, zinc hyaluronate, and aloe vera extract.

7. The composition having the effects of improving mitochondrial function and protecting microsphere structure according to claim 5, characterized in that: The whitening agent is at least one of glabridin, milk extract, daisy flower extract, azelaic acid, niacinamide, tranexamic acid, and ascorbyl glucoside.

8. The composition for improving mitochondrial function and protecting microsphere structure according to claim 5, characterized in that: The mitochondrial energy supplement is at least one of decapeptide-1, coenzyme Q10, α-lipoic acid, and nicotinamide adenine dinucleotide.

9. A method for preparing the composition having the effects of improving mitochondrial function and protecting microsphere structure according to any one of claims 1 to 8, characterized in that: The following steps are involved: Mixing the thickener with the polyether polyol moisturizer to obtain a mixture; After swelling the mixture, stirring and returning the temperature are performed in sequence to obtain a returned-temperature gel solution; adding an aqueous solution of a polysaccharide moisturizer to the warmed gel solution to obtain a first mixed gel solution; When the temperature of the first mixed gel solution naturally drops to 60° C., adding a preservative to obtain a second mixed gel solution; When the temperature of the second mixed gel solution naturally drops to 40° C., an aqueous solution of microspheroidized PDRN and an aqueous solution of other anti-aging active ingredients are added to obtain a third mixed gel solution; When the temperature of the third mixed gel solution naturally drops to 30° C., the pH value is detected to be 5.5-6.5, thereby obtaining the composition having the functions of improving mitochondrial function and protecting microsphere structure.

10. Use of the composition having the effects of improving mitochondrial function and protecting microsphere structure according to any one of claims 1 to 8 in the preparation of anti-aging skin care products.

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