Plasmonic-enhanced fluorescent miRNA nanoprobe with double-layered silica structure, and preparation method and application thereof

By designing a plasma-enhanced fluorescent miRNA nanoprobe with a bilayer silica structure, and utilizing the local surface plasmon resonance effect and graphene oxide quencher, a highly sensitive and low-cost intracellular miRNA detection was achieved, overcoming the limitations of traditional methods and providing a new biomarker for T cell differentiation.

CN120041188BActive Publication Date: 2025-11-28OCEAN UNIV OF CHINA
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Patent Information

Application Number
CN202510273259.1
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-03-10
Publication Date
2025-11-28
Estimated Expiration
2045-03-10

AI Technical Summary

Technical Problem

Existing technologies struggle to achieve high-sensitivity and low-cost detection of intracellular miRNAs, especially real-time monitoring of miRNA-21 and miRNA-150 during T cell differentiation. Traditional methods are time-consuming, complex to operate, and difficult to apply directly to intracellular detection.

Method used

A plasma-enhanced fluorescent miRNA nanoprobe with a bilayer silica structure was designed. By optimizing the separation distance between the fluorescent group and the gold nanoparticles, the fluorescence signal was enhanced by the local surface plasmon resonance effect. Graphene oxide was then used as a quencher to achieve quantitative detection of miRNA.

Benefits of technology

This study achieved highly sensitive and low-cost miRNA detection. The nanoprobe maintained 90% stability within 7 days, with a detection limit below 50 pM. It successfully monitored the expression changes of miRNA-21 and miRNA-150 during T cell differentiation, providing a new indicator for T cell phenotype identification.

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Abstract

The application discloses a kind of double-layer silicon dioxide structure's plasma enhanced fluorescence miRNA nanoprobe and its preparation method and application.The application uses the distance between the separation fluorescent group and nanometer gold particle by surface modification amino-modified silica coating layer, so as to achieve plasma enhancement effect;Then using the extinction peak generated when metal nanoparticle local surface plasmon resonance and the mutual overlap of fluorescence group (FITC or RITC) excitation peak or absorption peak, so as to further optimize plasma enhancement effect, and then prepare the plasma enhanced fluorescence miRNA nanoprobe with good stability, specificity and sensitivity, which can be used for quantitative detection miRNA-21 and miRNA-150 in T cell, for monitoring its differentiation state, for monitoring T cell phenotype and providing promising tool for promoting immunotherapy.
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Citation Information

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