Breeding device and method for microorganisms in distiller's yeast

Abstract

The invention discloses a device and a method for breeding microorganisms in distiller's yeast, belongs to the technical field of microorganism culture, and aims to solve the problem that when an existing microorganism culture device promotes uniform mixing of microorganisms, a nutrient solution and oxygen through stirring, the nutrient solution on the inner wall of the device is poor in fluidity, and the contact oxygen dissolving efficiency is affected. The reciprocating assembly drives the moving rod to push the shifting disc to move up and down in the cavity of the adjusting barrel and the space above the shifting disc, after the shifting disc moves out of the top port of the adjusting barrel, a nutrient solution carrying microorganisms is poured into the adjusting barrel, the shifting disc moves downwards to press the nutrient solution in the adjusting barrel, and the nutrient solution is squeezed and pushed to be sprayed out of the liquid spraying pipe at the bottom of the adjusting barrel. The sprayed fluid drives the raised microorganisms and nutrient solution to uprush; the nutrient solution in the cultivation cavity is circularly injected into the adjusting cylinder and then is extruded and sprayed out, so that the circulating flow rate of the nutrient solution adjacent to the bottom and the inner wall of the cultivation cavity is accelerated, the nutrient solution is fully contacted and fused with oxygen sprayed out of the breather pipe, and the influence on proliferation and growth of microorganisms due to insufficient oxygen supply of the microorganisms in the nutrient solution is avoided.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial culture, and specifically, to a device and method for breeding microorganisms in koji. Background Art

[0002] Koji, generally written as "jiuqu", is made by transferring the conidia of Aspergillus into strongly steamed white rice and then incubating it. Hyphae will grow lushly on the rice grains, which is koji. The amylase produced by Aspergillus will saccharify the starch in the rice. Therefore, since ancient times, it has been used as a raw material sugar together with malt to make wine, sweet wine, miso, etc. Koji is made by microbial fermentation. The quality of koji affects the brewing quality of products such as wine. Therefore, the cultivation of koji microorganisms is crucial, and it mainly relies on microbial cultivation devices for directional cultivation.

[0003] Chinese Patent CN215712935U discloses a microbial culture device, including a base. A rotating shaft is rotatably connected to the center position of the top of the base through a bearing. A culture chamber is hermetically rotatably connected to the top of the fixed shaft through a sealed bearing. On both sides of the inner cavity of the culture chamber, a stirring shaft and a rotating rod are respectively rotatably connected through sealed bearings. Stirring blades are arranged on the outside of the stirring shaft, and spiral blades are connected to the outer peripheral surface of the rotating rod. One side of the top of the base is connected with an L-shaped plate. The motor drives the microorganisms and the culture solution to rotate counterclockwise, drives a plurality of stirring blades to rotate clockwise, and at the same time drives the spiral blades to rotate. The rotation directions of the plurality of stirring blades are opposite to the rotation directions of the microorganisms and the culture solution, so as to realize full stirring of the microorganisms and the culture solution. The rotation of the spiral blades realizes driving the microorganisms and the culture solution to turn up and down, so that the microorganisms and the culture solution are fully mixed. When the existing microbial culture device passes oxygen into its nutrient solution, the rotation of the stirring rod is used to promote the uniform mixing of microorganisms, nutrient solution and oxygen. However, the fluidity of the nutrient solution at the inner wall of the culture device is slower than that at the center, and the contact effect with the introduced oxygen is poor, so as to affect the respiration and proliferation of the microorganisms inside.

[0004] Therefore, a device and method for breeding microorganisms in koji are introduced. Summary of the Invention

[0005] The purpose of the present invention is to provide a device and method for breeding microorganisms in koji, aiming to solve the problem in the above background art that when the existing microbial culture device promotes the uniform mixing of microorganisms, nutrient solution and oxygen by stirring, the fluidity of the nutrient solution at the inner wall of the device is poor, affecting its contact and oxygen dissolution efficiency.

[0006] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a device for breeding microorganisms in koji, comprising a bottom bin seat and a heat-insulating incubator fixedly connected to the top of the bottom bin seat, a cultivation chamber is arranged in the heat-insulating incubator, an oxygen inlet pipe is arranged on the outer wall of one side of the heat-insulating incubator, the oxygen inlet pipe is connected to a ventilation pipe arranged on the upper end of the bottom plate of the cultivation chamber, an adjusting cylinder is fixedly connected to the bottom plate of the cultivation chamber, a liquid spraying pipe is fixedly connected to the circumferential side wall of the adjusting cylinder near the bottom at uniform intervals, and a moving rod is movably penetrated on the bottom plate of the adjusting cylinder, a toggle disk is fixedly connected to the top of the moving rod, the toggle disk is movably engaged in the adjusting cylinder, the lower end of the moving rod extends through the inside of the bottom bin seat, and the bottom end of the moving rod is connected to a reciprocating assembly arranged on the bottom plate of the bottom bin seat, and the reciprocating assembly pushes the toggle disk to move up and down in the cavity of the adjusting cylinder and the space above through the moving rod to toggle the culture liquid in the cultivation chamber.

[0007] Furthermore, the liquid spray pipe includes a liquid outlet pipe fixedly connected to the side wall of the regulating cylinder and an arc-shaped acceleration tube fixedly connected to the end of the liquid outlet pipe, the end port of the arc-shaped acceleration tube is arranged obliquely upward, and a connected auxiliary branch pipe is arranged at the bottom of the liquid outlet pipe, and the end port of the auxiliary branch pipe is arranged obliquely downward.

[0008] Furthermore, a partition plate is fixedly connected between the inner walls in the middle of the thermal incubator, and the partition plate divides the interior of the thermal incubator into two incubation chambers of equal size. Constant temperature heating tubes are arranged on the inner walls at the four corners of each incubation chamber, and an aeration component is fixedly connected to the middle of the lower end of the top plate of the incubation chamber, and the lower end of the aeration component is suspended close to the top port of the regulating cylinder.

[0009] Furthermore, a drain valve tube is fixedly connected to the side wall of the cultivation chamber, a liquid storage tank is fixedly connected to the upper end of the top plate of the thermal insulation culture box corresponding to the cultivation chamber, and the liquid storage tank stores an acid-base regulator for adjusting the pH value of the nutrient solution. An electromagnetic valve tube is fixedly connected to the side wall of the liquid storage tank, and the end of the electromagnetic valve tube extends through the cultivation chamber.

[0010] Furthermore, the ventilation pipe includes a connecting pipe connected to the oxygen inlet pipe, the end of the connecting pipe passes through the side wall of the thermal insulation incubator and extends into the incubation chamber, and the end of the connecting pipe is connected to the side wall of the guide pipe arranged on the bottom plate of the incubation chamber. The guide pipe has a U-shaped structure, which surrounds the outside of the regulating cylinder, and a one-way jet pipe is fixedly connected to the outer wall of the guide pipe close to the regulating cylinder.

[0011] Furthermore, the reciprocating assembly includes a U-shaped bracket fixedly connected to the bottom plate of the bottom bin seat and a rotating shaft movably arranged through the side wall of the U-shaped bracket, the end of the rotating shaft between the upper and lower bottom plates of the U-shaped bracket is fixedly connected to a rotating disk, a driving block is fixedly connected to the upper edge of the outer wall of the rotating disk, a movable clamping sleeve limit frame is provided on the outer wall of the driving block, the bottom of the moving rod is fixedly connected to the middle of the top of the limit frame, the moving rod is movably arranged through the top plate of the U-shaped bracket, and a main gear is fixedly connected to the outer wall of the rotating shaft away from the rotating disk.

[0012] Furthermore, two identical groups of reciprocating components are arranged, which correspond to the incubation chambers respectively, and the main gears of the two groups of reciprocating components are connected by a transmission chain, and the end of the rotating shaft of one group of reciprocating components is connected to the output end of the suspension motor arranged on the bottom plate of the bottom bin seat.

[0013] Furthermore, the aeration assembly includes a positioning cylinder fixedly connected to the lower end of the incubation chamber top plate and a spiral groove opened on the inner wall of the positioning cylinder, a movable disk is movably engaged between the inner walls of the positioning cylinder, a limiting slider on the outer wall of one side of the movable disk is movably engaged in the spiral groove, and an elastic component is fixedly connected to the lower end of the incubation chamber top plate above the movable disk, the lower end of the elastic component is movably resting on the top of the movable disk, an air guide pipe is fixedly connected to the middle part of the lower end of the movable disk, the lower end of the air guide pipe passes through the bottom plate of the positioning cylinder and extends to the outside thereof, a flow equalizing disk is fixedly connected to the bottom thereof, air outlet nozzles are evenly spaced on the side walls of the flow equalizing disk, and dispersion rods are fixedly connected to the outer walls of the upper and lower ends of the tail of the air outlet nozzle.

[0014] Furthermore, the flow equalizing plate includes a hollow portion fixedly connected to the lower end of the air guide pipe and a mounting portion fixedly connected to the lower end of the hollow portion, the hollow portion is communicated with the air guide pipe, and an air outlet nozzle is arranged on its side wall, a limiting groove is opened in the middle of the lower end of the mounting portion, a movable ball is movably engaged in the limiting groove, and the movable ball moves and extends to the outside of the limiting groove port.

[0015] Preferably, the present invention provides another technical solution:

[0016] A method for implementing a microbial breeding device in distiller's yeast comprises the following steps:

[0017] S1: After the equipment is disinfected, the koji microorganisms and nutrient solution are mixed and injected into the culture chamber. At the same time, the oxygen pumped by the oxygen supply machine is disinfected and filtered and then injected into the nutrient solution in the culture chamber through the oxygen inlet pipe and the ventilation pipe;

[0018] S2: The reciprocating assembly starts and intermittently drives the moving rod to move up and down. When the moving rod moves up and down, the push-to-dial plate moves up and down in the adjusting cylinder cavity and the space above;

[0019] S3: After the adjusting cylinder is removed from the top port of the dial, the nutrient solution carrying microorganisms is poured into the adjusting cylinder. By moving the dial downward, the nutrient solution in the adjusting cylinder is compressed and pushed out from the liquid spraying pipe at the bottom of the adjusting cylinder.

[0020] S4: After the nutrient solution in the adjusting cylinder is sprayed out from the liquid spraying pipe, on the one hand, the fluid sprayed obliquely downward by the liquid spraying pipe impacts the microorganisms accumulated on the bottom plate of the cultivation chamber, causing them to rise. On the other hand, the fluid sprayed obliquely upward by the liquid spraying pipe drives the rising microorganisms and nutrient solution to surge upward.

[0021] S5: Repeat the above S2 - S4. The nutrient solution in the cultivation chamber carries the microorganisms inside and circulates into the adjusting cylinder and then is squeezed out, driving the circulation flow rate of the nutrient solution at the bottom and the adjacent inner wall of the cultivation chamber to accelerate, so that it can fully contact and blend with the oxygen sprayed out by the ventilation pipe.

[0022] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0023] A microorganism breeding device and method for koji provided by the present invention. The reciprocating component drives the moving rod to push the dial to intermittently move up and down in the cavity of the adjusting cylinder and the upper space. After the dial is removed from the top port of the adjusting cylinder, the nutrient solution carrying microorganisms is poured into the adjusting cylinder. By moving the dial downward, the nutrient solution in the adjusting cylinder is compressed and pushed out from the liquid spraying pipe at the bottom of the adjusting cylinder. After the nutrient solution in the adjusting cylinder is sprayed out obliquely downward from the liquid spraying pipe, it stirs up the microorganisms accumulated on the bottom plate of the cultivation chamber, and the fluid sprayed obliquely upward drives the rising microorganisms and nutrient solution to surge upward; the nutrient solution in the cultivation chamber carries the microorganisms inside and circulates into the adjusting cylinder and then is squeezed out, driving the circulation flow rate of the nutrient solution at the bottom and the adjacent inner wall of the cultivation chamber to accelerate, so that it can fully contact and blend with the oxygen sprayed out by the ventilation pipe, avoiding the influence on the proliferation and growth of microorganisms in the nutrient solution due to insufficient oxygen supply. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 is the overall structural schematic diagram of the present invention;

[0025] Figure 2 is the sectional structural schematic diagram of the present invention;

[0026] Figure 3 is the installation structural schematic diagram of the reciprocating component and the dial of the present invention;

[0027] Figure 4 is the schematic diagram of the flow direction of the nutrient solution driven by the adjusting cylinder and the dial of the present invention;

[0028] Figure 5 is the front - view perspective structural schematic diagram of the reciprocating component of the present invention;

[0029] Figure 6 is the rear - view perspective structural schematic diagram of the reciprocating component of the present invention;

[0030] Figure 7 Schematic diagram of the vent pipe structure of the present invention;

[0031] Figure 8 Schematic diagram of the gas charging component structure of the present invention;

[0032] Figure 9 Partial sectional view structure schematic diagram of the gas charging component of the present invention;

[0033] Figure 10 Schematic diagram of the flow equalizing plate structure of the present invention;

[0034] Figure 11 For the present invention Figure 9 Enlarged structure schematic diagram at position A.

[0035] In the figure: 1. Incubator; 11. Partition board; 12. Constant temperature heating tube; 13. Gas charging component; 131. Positioning cylinder; 132. Spiral chute; 133. Moving disk; 134. Limit slider; 135. Elastic component; 136. Air guide pipe; 137. Flow equalizing plate; 1371. Hollow part; 1372. Installation part; 1373. Limit groove; 1374. Moving ball; 138. Air outlet spray pipe; 139. Dispersion rod; 14. Drain valve pipe; 15. Liquid storage tank; 16. Solenoid valve pipe; 2. Bottom bin seat; 3. Oxygen inlet pipe; 4. Vent pipe; 41. Connecting pipe; 42. Diversion pipe; 43. One-way spray pipe; 5. Cultivation chamber; 6. Adjusting cylinder; 7. Liquid spray pipe; 71. Liquid outlet pipe; 72. Arc-shaped accelerating pipe; 73. Auxiliary branch pipe; 8. Moving rod; 9. Dialing disk; 10. Reciprocating component; 101. U-shaped bracket; 102. Rotating shaft; 103. Rotating disk; 104. Driving block; 105. Limit frame; 106. Main gear; 107. Transmission chain. Detailed implementation manners

[0036] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0037] Please refer to Figure 1 and Figure 2A device for breeding microorganisms in wine yeast comprises a bottom bin seat 2 and a heat preservation incubator 1 fixedly connected to the top of the bottom bin seat 2, movable wheels are respectively welded at the four corners of the bottom of the bottom bin seat 2, a controller is arranged on the outer wall of the front end cover of the heat preservation incubator 1, the controller is used to control the operation of the equipment and to display the internal environment monitoring data of the heat preservation incubator 1 in real time, a culture chamber 5 is arranged in the heat preservation incubator 1, an oxygen inlet pipe 3 is arranged on the outer wall of one side of the heat preservation incubator 1, the air inlet end of the oxygen inlet pipe 3 is connected to the oxygen supply machine and the disinfection and filtering device, and the oxygen inlet pipe 3 is connected to the ventilation pipe 4 arranged on the upper end of the bottom plate of the culture chamber 5.

[0038] A partition plate 11 is fixedly connected between the inner walls at the middle of the insulated incubator 1, and the partition plate 11 divides the interior of the insulated incubator 1 into two cultivation chambers 5 of the same size. A plurality of cultivation chambers 5 can simultaneously breed and cultivate different types of koji microorganisms. A constant temperature heating tube 12 is arranged on the inner wall at the four corners of each cultivation chamber 5, and a detection sensor (not shown, mainly composed of a temperature sensor, an oxygen concentration sensor, and a pH value sensor, etc.) is arranged on the inner wall of the cultivation chamber 5. A drain valve pipe 14 and an exhaust valve are fixedly connected to the side wall of the cultivation chamber 5. The exhaust valve is arranged It is placed on the side wall of the cultivation chamber 5 near the top, and the upper end of the top plate of the heat preservation incubator 1 corresponding to the cultivation chamber 5 is fixedly connected with a liquid storage tank 15, and the liquid storage tank 15 stores an acid-base regulator for adjusting the pH value of the nutrient solution. An electromagnetic valve tube 16 is fixedly connected to the side wall of the liquid storage tank 15, and the end of the electromagnetic valve tube 16 extends into the cultivation chamber 5. The electromagnetic valve tube 16 maintains the stability of the pH value of the nutrient solution by quantitatively adding the acid-base regulator in the liquid storage tank 15 to the nutrient solution in the cultivation chamber 5, so as to ensure a suitable pH value proliferation environment for the koji microorganisms in the nutrient solution.

[0039] In order to solve the problem that the nutrient solution on the inner wall of the existing microbial culture device has poor fluidity and affects its contact with dissolved oxygen efficiency when the microorganisms, nutrient solution and oxygen are uniformly mixed by stirring, please refer to Figures 1-7 , provide the following preferred technical solutions:

[0040] An adjusting cylinder 6 is fixedly connected to the bottom plate of the cultivation chamber 5, and liquid spraying pipes 7 are fixedly connected to the circumferential side walls of the adjusting cylinder 6 at even intervals near the bottom. A moving rod 8 is movably provided on the bottom plate of the adjusting cylinder 6, and a toggle disk 9 is fixedly connected to the top of the moving rod 8. The toggle disk 9 is movably engaged in the adjusting cylinder 6. The lower end of the moving rod 8 extends through the inside of the bottom bin seat 2, and the bottom end thereof is connected to a reciprocating assembly 10 arranged on the bottom plate of the bottom bin seat 2. The reciprocating assembly 10 pushes the toggle disk 9 to move up and down in the cavity of the adjusting cylinder 6 and the space above through the moving rod 8, so as to toggle the culture liquid in the cultivation chamber 5.

[0041] The liquid spraying pipe 7 includes a liquid outlet pipe 71 fixedly connected to the side wall of the adjusting cylinder 6 and an arc-shaped accelerating pipe 72 fixedly connected to the end of the liquid outlet pipe 71. The end port of the arc-shaped accelerating pipe 72 is arranged obliquely upward. An auxiliary branch pipe 73 is connected and communicated at the bottom of the liquid outlet pipe 71, and the end port of the auxiliary branch pipe 73 is arranged obliquely downward.

[0042] The ventilation pipe 4 includes a connecting pipe 41 connected and communicated with the oxygen inlet pipe 3. The end of the connecting pipe 41 penetrates through the side wall of the insulation incubator 1 and extends into the cultivation chamber 5. Its end is connected to the side wall of a diversion pipe 42 arranged on the bottom plate of the cultivation chamber 5. The diversion pipe 42 has a square structure and surrounds the outside of the adjusting cylinder 6. A one-way air spraying pipe 43 is fixedly connected to the outer wall of the diversion pipe 42 on the side close to the adjusting cylinder 6.

[0043] The reciprocating assembly 10 includes a U-shaped bracket 101 fixedly connected to the bottom plate of the bottom bin seat 2 and a rotating shaft 102 movably penetrating through the side wall of the U-shaped bracket 101. A rotating disk 103 is fixedly connected to the end of the rotating shaft 102 between the upper and lower bottom plates of the U-shaped bracket 101. A driving block 104 is fixedly connected to the upper edge of the outer wall of the rotating disk 103. A limiting frame 105 is movably sleeved on the outer wall of the driving block 104. The middle part of the top end of the limiting frame 105 is fixedly connected to the bottom of the moving rod 8. The moving rod 8 movably penetrates through the top plate of the U-shaped bracket 101. A main gear 106 is fixedly connected to the outer wall of the rotating shaft 102 at the end far from the rotating disk 103.

[0044] There are two identical sets of reciprocating assemblies 10, which are respectively arranged corresponding to the cultivation chamber 5. The main gears 106 of the two sets of reciprocating assemblies 10 are sleeved by a transmission chain 107. The end of the rotating shaft 102 of one set of reciprocating assemblies 10 is connected to the output end of a suspension motor arranged on the bottom plate of the bottom bin seat 2.

[0045] Specifically, after disinfecting the equipment, the koji microorganisms and the nutrient solution are mixed and then injected into the cultivation chamber 5. At the same time, the oxygen pumped by the oxygen supply machine is filtered through disinfection and then injected into the nutrient solution in the cultivation chamber 5 through the oxygen inlet pipe 3 and the ventilation pipe 4. The rotation motor is started to drive the rotating disk 103 to rotate in cooperation with the rotating shaft 102. The rotating shaft 102 connected to the rotation motor drives another group of rotating disks 103 to rotate synchronously through the main gear 106 and the transmission chain 107. When the rotating disk 103 rotates, the driving block 104 is used to drive the limiting frame 105 to move intermittently up and down between the upper and lower bottom plates of the U-shaped bracket 101, so as to drive the moving rod 8 to push the dial 9 to move up and down in the cavity and the upper space of the adjusting cylinder 6. After the dial 9 moves out of the top port of the adjusting cylinder 6, the nutrient solution carrying microorganisms is poured into the adjusting cylinder 6. By means of the downward movement of the dial 9, the nutrient solution in the adjusting cylinder 6 is compressed, and is squeezed out from the liquid spraying pipe 7 at the bottom of the adjusting cylinder 6. After the nutrient solution in the adjusting cylinder 6 is sprayed obliquely downward from the liquid spraying pipe 7, it agitates the microorganisms accumulated on the bottom plate of the cultivation chamber 5. The fluid sprayed obliquely upward drives the lifted microorganisms and nutrient solution to surge upward; the nutrient solution in the cultivation chamber 5 carries the microorganisms inside and circulates into the adjusting cylinder 6 and then is squeezed out, driving the circulation flow rate of the nutrient solution at the bottom and the adjacent inner wall of the cultivation chamber 5 to accelerate, so that it fully contacts and merges with the oxygen sprayed from the ventilation pipe 4, strengthening the fusion effect of the nutrient solution on the oxygen, thereby ensuring the aerobic demand of the microorganisms in all the nutrient solutions in the cultivation chamber 5 and avoiding the problem that the microorganisms in the local nutrient solution are affected by insufficient oxygen supply and affect proliferation.

[0046] Furthermore, to solve the problem that adding ordinary acid-base regulators cannot improve the pH value of the nutrient solution, the following preferred solution is provided in this embodiment:

[0047] As Figure 2 、 Figure 3 and Figures 8-11 shown, a gas adding component 13 is fixedly connected to the middle part at the lower end of the top plate of the cultivation chamber 5, and the lower end of the gas adding component 13 is suspended close to the upper part of the top port of the adjusting cylinder 6.

[0048] The gas charging assembly 13 includes a positioning cylinder 131 fixedly connected to the lower end of the top plate of the cultivation chamber 5 and a spiral chute 132 formed on the inner wall of the positioning cylinder 131. The top end of the positioning cylinder 131 is communicated with an air delivery pipe arranged on the top of the incubator 1. A moving disk 133 is movably engaged between the inner walls of the positioning cylinder 131. A limiting slider 134 on the outer wall of one side of the moving disk 133 is movably engaged in the spiral chute 132. A resilient member 135 is fixedly connected to the lower end of the top plate of the cultivation chamber 5 above the moving disk 133. The lower end of the resilient member 135 is movably leaned against the top of the moving disk 133. When the resilient member 135 maintains a normal diastolic state, the lower end of the moving disk 133 is in contact with the bottom plate of the positioning cylinder 131. A gas guide pipe 136 is fixedly connected to the middle of the lower end of the moving disk 133. The top port of the gas guide pipe 136 extends to the top surface of the moving disk 133. The lower end of the gas guide pipe 136 penetrates through the bottom plate of the positioning cylinder 131 and extends to the outside thereof, and a flow equalizing disk 137 is fixedly connected to its bottom. Air outlet nozzles 138 are evenly spaced on the side wall of the flow equalizing disk 137. Dispersion rods 139 are fixedly connected to the upper and lower outer walls at the tail of the air outlet nozzles 138 respectively.

[0049] The flow equalizing disk 137 includes a hollow part 1371 fixedly connected to the lower end of the gas guide pipe 136 and a mounting part 1372 fixedly connected to the lower end of the hollow part 1371. The hollow part 1371 is communicated with the gas guide pipe 136, and the air outlet nozzles 138 are arranged on its side wall. A limiting groove 1373 is formed in the middle of the lower end of the mounting part 1372. A moving ball 1374 is movably engaged in the limiting groove 1373, and the moving ball 1374 moves and extends outside the port of the limiting groove 1373.

[0050] Specifically, when adding ordinary acid-base regulators to the nutrient solution cannot improve its pH value, after removing the top port of the regulating cylinder 6 on the dial 9, the dial 9 pushes the flow equalizing plate 137 upward. The flow equalizing plate 137 uses the air guide pipe 136 to push the moving plate 133 to move upward along the inner wall of the positioning cylinder 131. At the same time, the limiting slider 134 on the side wall of the moving plate 133 slides along the spiral chute 132 on the inner wall of the positioning cylinder 131, so that the moving plate 133 drives the flow equalizing plate 137 to rotate while moving upward under the push of the dial 9 based on the moving ball 1374. The flow equalizing plate 137 drives the air outlet nozzle 138 and the dispersion rod 139; conversely, when the dial 9 moves downward, the elastic member 135 pushes the moving plate 133 downward to drive the flow equalizing plate 137 to rotate in the reverse direction. At this time, carbon dioxide gas and other gases for adjusting the pH value are introduced into the positioning cylinder 131 through an external air inlet pipe. After the carbon dioxide and other gases enter the hollow part 1371 along the air guide pipe 136, they are sprayed into the nutrient solution in the cultivation chamber 5 through the air outlet nozzle 138. By moving the dial 9 up and down, the flow equalizing plate 137 is synchronously driven to rotate up and down in the nutrient solution, so as to introduce carbon dioxide and other gases into the nutrient solution in all directions. When the gas is sprayed, it is rotated and stirred by the dispersion rod 139 at the port of the air outlet nozzle 138, and can be quickly dissolved in the nutrient solution, improving the dispersion and fusion efficiency after the gas is concentratedly introduced, and realizing the rapid adjustment of the pH of the nutrient solution.

[0051] To better display the microbial breeding device in the koji, this embodiment provides an implementation method of the microbial breeding device in the koji:

[0052] An implementation method of the microbial breeding device in the koji includes the following implementation steps:

[0053] Step 1: After disinfecting the equipment, mix the koji microorganisms and the nutrient solution and inject them into the cultivation chamber 5. At the same time, the oxygen pumped by the oxygen supply machine is filtered through disinfection and then injected into the nutrient solution in the cultivation chamber 5 through the oxygen inlet pipe 3 and the air guide pipe 4.

[0054] Step 2: The reciprocating assembly 10 starts to intermittently drive the moving rod 8 to move up and down. When the moving rod 8 moves up and down, it pushes the dial 9 to move up and down in the cavity of the regulating cylinder 6 and the upper space.

[0055] Step 3: After the dial 9 moves out of the top port of the regulating cylinder 6, the nutrient solution carrying microorganisms is poured into the regulating cylinder 6. By pressing the dial 9 downward, the nutrient solution in the regulating cylinder 6 is compressed and pushed out from the liquid spraying pipe 7 at the bottom of the regulating cylinder 6.

[0056] Step 4: After the nutrient solution in the regulating cylinder 6 is sprayed out from the liquid spraying pipe 7, on the one hand, the fluid sprayed obliquely downward by the liquid spraying pipe 7 impacts the microorganisms accumulated on the bottom plate of the cultivation chamber 5 to make them rise, and on the other hand, the fluid sprayed obliquely upward by the liquid spraying pipe 7 drives the rising microorganisms and the nutrient solution to surge upward.

[0057] Step Five: Repeat the above Step One to Step Four, so that the nutrient solution in the cultivation chamber 5 carrying the microorganisms inside circulates into the adjustment cylinder 6 and then is extruded and sprayed out, driving the circulation flow rate of the nutrient solution at the bottom and the adjacent inner wall of the cultivation chamber 5 to increase, so that it can fully contact and blend with the oxygen ejected from the air pipe 4.

[0058] It should be noted that in this text, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the term "comprising", "including" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device comprising a series of elements not only includes those elements, but also includes other elements not expressly listed, or further includes elements inherent to such process, method, article or device.

[0059] Although the embodiments of the present invention have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A device for breeding microorganisms in distiller's yeast, comprising a bottom bin seat (2) and a heat preservation incubator (1) fixedly connected to the top of the bottom bin seat (2), a cultivation chamber (5) being arranged in the heat preservation incubator (1), an oxygen inlet pipe (3) being arranged on an outer wall of one side of the heat preservation incubator (1), the oxygen inlet pipe (3) being connected to a ventilation pipe (4) arranged on the upper end of the bottom plate of the cultivation chamber (5), characterized in that: The bottom plate of the cultivation chamber (5) is fixedly connected with an adjusting cylinder (6), and liquid spraying pipes (7) are evenly spaced and fixedly connected to the circumferential side wall of the adjusting cylinder (6) near the bottom. A moving rod (8) is movably provided on the bottom plate of the adjusting cylinder (6), and a toggle plate (9) is fixedly connected to the top of the moving rod (8). The toggle plate (9) is movably engaged in the adjusting cylinder (6). The lower end of the moving rod (8) extends through the inside of the bottom bin seat (2), and the bottom end of the moving rod (8) is connected to a reciprocating assembly (10) provided on the bottom plate of the bottom bin seat (2). The reciprocating assembly (10) pushes the toggle plate (9) to move up and down in the cavity of the adjusting cylinder (6) and the space above through the moving rod (8) to toggle the culture liquid in the cultivation chamber (5).

2. The device for breeding microorganisms in distiller's yeast according to claim 1, characterized in that: The liquid spray pipe (7) comprises a liquid outlet pipe (71) fixedly connected to the side wall of the regulating cylinder (6) and an arc-shaped accelerating pipe (72) fixedly connected to the end of the liquid outlet pipe (71), the end port of the arc-shaped accelerating pipe (72) is arranged obliquely upward, and a connected auxiliary branch pipe (73) is arranged at the bottom of the liquid outlet pipe (71), and the end port of the auxiliary branch pipe (73) is arranged obliquely downward.

3. The device for breeding microorganisms in distiller's yeast according to claim 1, characterized in that: A partition plate (11) is fixedly connected between the inner walls at the middle of the heat preservation incubator (1), and the partition plate (11) divides the interior of the heat preservation incubator (1) into two cultivation chambers (5) of the same size. A constant temperature heating pipe (12) is arranged on the inner wall at the four corners of each cultivation chamber (5). An aeration component (13) is fixedly connected to the middle of the lower end of the top plate of the cultivation chamber (5), and the lower end of the aeration component (13) is suspended close to the top port of the regulating cylinder (6).

4. The device for breeding microorganisms in distiller's yeast according to claim 3, characterized in that: A liquid discharge valve tube (14) is fixedly connected to the side wall of the cultivation chamber (5); a liquid storage tank (15) is fixedly connected to the upper end of the top plate of the heat preservation incubator (1) corresponding to the cultivation chamber (5); an acid-base regulator for adjusting the pH value of the nutrient solution is stored in the liquid storage tank (15); an electromagnetic valve tube (16) is fixedly connected to the side wall of the liquid storage tank (15); and a terminal end of the electromagnetic valve tube (16) extends through and into the cultivation chamber (5).

5. The device for breeding microorganisms in distiller's yeast according to claim 3, characterized in that: The ventilation pipe (4) comprises a connecting pipe (41) connected to the oxygen inlet pipe (3); the end of the connecting pipe (41) penetrates the side wall of the heat preservation incubator (1) and extends into the incubation chamber (5); the end of the connecting pipe (41) is connected to the side wall of a flow guide pipe (42) arranged on the bottom plate of the incubation chamber (5); the flow guide pipe (42) is in a square-shaped structure, surrounds the outside of the regulating cylinder (6), and a one-way air injection pipe (43) is fixedly connected to the outer wall of the flow guide pipe (42) on the side close to the regulating cylinder (6).

6. The device for breeding microorganisms in distiller's yeast according to claim 3, characterized in that: The reciprocating assembly (10) comprises a U-shaped bracket (101) fixedly connected to the bottom plate of the bottom bin seat (2) and a rotating shaft (102) movably penetrating the side wall of the U-shaped bracket (101); a rotating disk (103) is fixedly connected to the end of the rotating shaft (102) between the upper and lower bottom plates of the U-shaped bracket (101); a driving block (104) is fixedly connected to the upper edge of the outer wall of the rotating disk (103); a movable clamping sleeve limit frame (105) is provided on the outer wall of the driving block (104); a bottom of a moving rod (8) is fixedly connected to the middle of the top of the limit frame (105); the moving rod (8) is movably penetrating the top plate of the U-shaped bracket (101); and a main gear (106) is fixedly connected to the outer wall of the rotating shaft (102) at one end away from the rotating disk (103).

7. The device for breeding microorganisms in distiller's yeast according to claim 6, characterized in that: The reciprocating components (10) are provided in two identical groups, which are respectively provided in correspondence with the culture chambers (5), and the main gears (106) of the two groups of the reciprocating components (10) are connected with each other through a transmission chain (107), and the end of the rotating shaft (102) of one group of the reciprocating components (10) is connected to the output end of the suspension motor provided on the bottom plate of the bottom bin seat (2).

8. The device for breeding microorganisms in distiller's yeast according to claim 7, characterized in that: The gas filling assembly (13) comprises a positioning cylinder (131) fixedly connected to the lower end of the top plate of the cultivation chamber (5) and a spiral slide groove (132) provided on the inner wall of the positioning cylinder (131); a movable disk (133) is movably engaged between the inner walls of the positioning cylinder (131); a limiting slider (134) on the outer wall of one side of the movable disk (133) is movably engaged in the spiral slide groove (132); and an elastic component (135) is fixedly connected to the lower end of the top plate of the cultivation chamber (5) above the movable disk (133). The lower end of the elastic component (135) is movably placed on the top of the movable plate (133); an air guide tube (136) is fixedly connected to the middle of the lower end of the movable plate (133); the lower end of the air guide tube (136) passes through the bottom plate of the positioning tube (131) and extends to the outside thereof; a flow equalizing plate (137) is fixedly connected to the bottom thereof; air outlet nozzles (138) are evenly spaced on the side wall of the flow equalizing plate (137); and dispersion rods (139) are fixedly connected to the outer walls of the upper and lower ends of the tail of the air outlet nozzle (138), respectively.

9. The device for breeding microorganisms in distiller's yeast according to claim 8, characterized in that: The flow balancing plate (137) comprises a hollow portion (1371) fixedly connected to the lower end of the air guide pipe (136) and a mounting portion (1372) fixedly connected to the lower end of the hollow portion (1371); the hollow portion (1371) is connected to the air guide pipe (136), and an air outlet nozzle (138) is arranged on its side wall; a limiting groove (1373) is provided at the middle of the lower end of the mounting portion (1372); a movable ball (1374) is movably engaged in the limiting groove (1373); and the movable ball (1374) moves and extends to the outside of the port of the limiting groove (1373).

10. The implementation method of the microorganism breeding device in distiller's yeast according to claim 9, characterized in that: The implementation steps include: S1: After the equipment is disinfected, the koji microorganisms and the nutrient solution are mixed and injected into the culture chamber (5). At the same time, the oxygen pumped by the oxygen supply machine is disinfected and filtered and then injected into the nutrient solution in the culture chamber (5) through the oxygen inlet pipe (3) and the ventilation pipe (4); S2: The reciprocating assembly (10) starts to intermittently drive the moving rod (8) to move up and down. When the moving rod (8) moves up and down, it pushes the dial (9) to move up and down in the cavity of the adjustment tube (6) and the space above; S3: After the toggle plate (9) is moved upward out of the top port of the regulating cylinder (6), the nutrient solution carrying the microorganisms is poured into the regulating cylinder (6), and the nutrient solution in the regulating cylinder (6) is pressed downward by the toggle plate (9), so that the nutrient solution is ejected from the spray pipe (7) at the bottom of the regulating cylinder (6); S4: After the nutrient solution in the regulating cylinder (6) is sprayed out from the spray pipe (7), on the one hand, the fluid sprayed obliquely downward from the spray pipe (7) impacts the microorganisms accumulated on the bottom plate of the cultivation chamber (5) to lift them up, and on the other hand, the fluid sprayed obliquely upward from the spray pipe (7) drives the lifted microorganisms and nutrient solution to surge upward; S5: Repeat the above steps S2-S4, the nutrient solution in the culture chamber (5) carries the microorganisms therein and circulates into the regulating cylinder (6) and is then squeezed and sprayed out, thereby accelerating the circulation flow rate of the nutrient solution at the bottom and adjacent to the inner wall of the culture chamber (5), so that it can fully contact and fuse with the oxygen sprayed from the ventilation pipe (4).

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