Anti-aging composition and application thereof

Abstract

The present invention relates to an anti-aging composition for extending the length of telomeres in a mammalian subject. The anti-aging composition comprises the following components in percentage by weight: 5-20% of a radix salviae miltiorrhizae extract, 25-40% of an alpha amylase inhibitor and 50-60% of probiotics, the probiotics comprise bifidobacterium animalis and lactobacillus rhamnosus, and in each gram of the anti-aging composition, the total bacterial count of the bifidobacterium animalis and the lactobacillus rhamnosus is 1 * 10 < 10 > CFU. The anti-aging composition can prolong the length of telomeres of a mammal individual and effectively resist aging.

Description

Technical Field

[0001] The present invention relates to an anti-aging composition, and particularly to an anti-aging composition and its use. Background Art

[0002] Aging refers to a process of growing old, which is widely used to describe humans, animals, fungi, etc. Generally, the length of telomeres is considered the best biomarker for aging. When the length of telomeres shortens to a certain extent, it will be unable to protect the integrity of chromosomes, resulting in the problem of chromosome instability. Therefore, cells will gradually enter the path of senescence or apoptosis.

[0003] Although sufficient sleep, regular exercise, and appropriate stress relief can be used to extend the length of telomeres, there is no composition with anti-aging effects on the market. In view of this, there is still a need to provide an anti-aging composition and its use. Summary of the Invention

[0004] (I) Technical Problems to be Solved

[0005] In view of the above-mentioned disadvantages and deficiencies of the prior art, the purpose of the present invention is to provide an anti-aging composition, specifically a composition with NRF mitochondrial activation components and the functions of telomere extension, anti-aging, and cell rejuvenation, for extending the length of telomeres in mammalian individuals.

[0006] The secondary purpose of the present invention is to provide a use of an anti-aging composition, which can effectively extend the length of telomeres by means of the active ingredients contained in the anti-aging composition.

[0007] (II) Technical Solutions

[0008] The quantifiers "a" or "an" used for the elements and components described throughout the present invention are only for convenience and provide the general meaning of the scope of the present invention; in the present invention, it should be construed as including one or at least one, and the single concept also includes the plural case, unless it clearly means otherwise.

[0009] The anti-aging composition of the present invention may include: a Salvia miltiorrhiza extract at 5-20% by weight, an α-amylase inhibitor at 25-40%, and a probiotic at 50-60%. The probiotic includes Bifidobacterium animalis and Lactobacillus rhamnosus, and in each gram of the anti-aging composition, the total number of bacteria of Bifidobacterium animalis and Lactobacillus rhamnosus is 1×10 10 CFU. For example, in each gram of the anti-aging composition, the number of bacteria of Bifidobacterium animalis and Lactobacillus rhamnosus may be respectively between 2×10 9~8×10 9 CFU, and the Salvia miltiorrhiza extract can be obtained by water-extracting a root sample of Salvia miltiorrhiza at a temperature of 70-100°C.

[0010] Accordingly, for the anti-aging composition of the present invention, the mitochondrial activity of the mammalian individual is activated by the Salvia miltiorrhiza extract, and the energy generation of cells is regulated to ensure sufficient energy for DNA repair and telomere maintenance. And by the activity of the α-amylase inhibitor, the starch ingested by the mammalian individual is inhibited from being decomposed into glucose, so that the absorption of sugars can be reduced, and the probiotic can adjust the microbial flora of the digestive tract of the mammalian individual. Through the synergistic effect of the foregoing components, the length of telomeres can be jointly extended, and thus the anti-aging effect can be achieved.

[0011] The anti-aging composition of the present invention may further include an extract in an amount of 1-10% by weight, and the extract may be selected from at least one of an alfalfa extract, a bitter gourd extract, a smoked plum extract, a strawberry extract, a rhodiola extract, a chrysanthemum extract, and a mulberry leaf extract. Thus, by the addition of the alfalfa extract, it can act together with the Salvia miltiorrhiza extract to activate mitochondria, and by the addition of the bitter gourd extract, the smoked plum extract, the strawberry extract, the rhodiola extract, the chrysanthemum extract, and the mulberry leaf extract, it helps to inhibit the absorption of sugars.

[0012] The use of the anti-aging composition of the present invention as described above is to prepare an anti-aging drug, wherein the anti-aging composition is for administration to a mammalian individual, for example, it can be administered to the mammalian individual in an oral manner. For example, when the mammalian individual is a human individual, for a person weighing 75 kg, it can be administered to the human individual at a dose of 4-6 grams per day and continuously administered for 2 months; when the mammalian is a dog individual or a cat individual, for an individual weighing 25 kg, it can be administered to the dog individual (or the cat individual) at a dose of 1-2 grams per day and continuously administered for 1 month.

[0013] Accordingly, the use of the anti-aging composition is to effectively extend the length of telomeres in mammalian individuals through the synergistic use of the Salvia miltiorrhiza extract, the α-amylase inhibitor, and the probiotic, thereby delaying the aging of mammalian individuals, which is the efficacy of the present invention.

[0014] (III) Beneficial effects

[0015] The beneficial effect of the present invention is that the use of the anti-aging composition can effectively extend the length of telomeres through the synergistic use of the Salvia miltiorrhiza extract, the α-amylase inhibitor, and the probiotic, thereby delaying the aging of mammalian individuals. Detailed implementation manners

[0016] To make the above and other objects, features, and advantages of the present invention more obvious and understandable, the following provides a detailed description of the preferred embodiments of the present invention.

[0017] An anti-aging composition according to an embodiment of the present invention may include: a Salvia miltiorrhiza extract, an α-amylase inhibitor, and a probiotic powder. By the combined action of the Salvia miltiorrhiza extract, the α-amylase inhibitor, and the probiotic powder, the length of telomeres of mammalian individuals can be extended, thereby achieving an anti-aging effect.

[0018] Specifically, the anti-aging composition may contain 5-20% by weight of the Salvia miltiorrhiza extract. The Salvia miltiorrhiza extract may preferably be prepared by a method comprising the following steps: providing the Salvia miltiorrhiza sample; extracting the Salvia miltiorrhiza sample with water as an extraction solvent; and concentrating a crude Salvia miltiorrhiza extract to obtain the Salvia miltiorrhiza extract.

[0019] Preferably, the roots of Salvia miltiorrhiza can be taken as the Salvia miltiorrhiza sample, and before extraction with water, the Salvia miltiorrhiza sample is pre-dried to obtain a dried Salvia miltiorrhiza product with a moisture content equal to or less than 5%.

[0020] For example, every 50 grams of the Salvia miltiorrhiza sample can be mixed with 1,000 milliliters of the extraction solvent to obtain a mixture, and then extracted at a temperature of about 100°C for a total extraction time of up to 60 minutes, so that the active ingredients rich in the Salvia miltiorrhiza sample can be completely dissolved in the extraction solvent. This is widely used by those with ordinary knowledge in the technical field to which the present invention belongs and will not be elaborated here.

[0021] The aforementioned crude Salvia miltiorrhiza extract can be cooled and filtered, and finally freeze-dried to obtain a Salvia miltiorrhiza extract with a water content of less than 5%. Through this procedure, the active ingredients of the Salvia miltiorrhiza extract can be more concentrated, so that only a small amount of the Salvia miltiorrhiza extract is needed to exert the best therapeutic effect.

[0022] The anti-aging composition may further include 25-40% by weight of an α-amylase inhibitor, which can be derived from plants such as wheat, barley, millet, sorghum, peanut, and soybean. In this embodiment, the α-amylase inhibitor from wheat is used, and its half-maximal inhibitory concentration (IC 50 ) is at least 0.6 μg / g.

[0023] The anti-aging composition may further include 50-60% by weight of probiotics, which may include Bifidobacterium animalis subsp. lactis and Lacticaseibacillus rhamnosus. For example, the Bifidobacterium animalis can be purchased from the Bioresource Collection and Research Center, and its numbers can be BCRC 17394, BCRC 80706, BCRC 80707, BCRC 80708, BCRC 80709, BCRC 80710, BCRC 81187, etc. The Lacticaseibacillus rhamnosus can also be optionally purchased from the Bioresource Collection and Research Center, and its numbers can be BCRC 10940, BCRC 11069, BCRC 11197, BCRC 11673, BCRC 12249, BCRC 14027, BCRC 14029, BCRC 81178, BCRC 80667, BCRC 80666, BCRC 80665, BCRC 80664, BCRC 80663, BCRC 80065, BCRC 14068, BCRC 16000, BCRC 16095, BCRC 17004, BCRC 17006, BCRC 17007, BCRC 17490, etc. It should be noted that the microorganism can be a viable microorganism or a heat-inactivated microorganism (i.e., the secondary metabolite of the microorganism), which can be understood by those with ordinary knowledge in the technical field to which the present invention pertains and is not limited herein.

[0024] For the effect of the anti-aging composition, it is preferably ensured that in each gram of the anti-aging composition, the total number of bacteria of the Bifidobacterium animalis and the Lacticaseibacillus rhamnosus reaches 1×10 10 CFU. For example, the number of bacteria of the Bifidobacterium animalis and the Lacticaseibacillus rhamnosus can be respectively between 2×10 9 ~8×10 9 CFU, so that the ratio of the number of bacteria of the Bifidobacterium animalis and the Lacticaseibacillus rhamnosus can be between 1:4 and 4:1.

[0025] In addition, the anti-aging composition may further include an extract in an amount of 1 to 10% by weight. The extract may be selected from at least one of an extract of Medicago sativa, an extract of Momordica charantia, an extract of smoked Prunus mume fruits, an extract of Fragaria×amamassa, an extract of Rhodiola rosea, an extract of Chrysanthemum×morifolium, and an extract of Morus leaves. The Medicago sativa extract may act together with the Salvia miltiorrhiza extract to activate mitochondria, while the Momordica charantia extract, the smoked Prunus mume fruits extract, the Fragaria×amamassa extract, the Rhodiola rosea extract, the Chrysanthemum×morifolium extract, and the Morus leaves extract have the activity of reducing sugar absorption.

[0026] The anti-aging composition can be administered to a mammalian individual (e.g., a human of the order Primates or a cat or dog of the order Carnivora, etc.) to activate the mitochondrial activity of the mammalian individual through the Salvia miltiorrhiza extract, regulate the energy generation of cells, ensure sufficient energy for DNA repair and telomere maintenance, and inhibit the decomposition of starch ingested by the mammalian individual into glucose through the activity of the α-amylase inhibitor, thereby reducing sugar absorption. The probiotic can adjust the microbiota of the digestive tract of the mammalian individual, thereby extending the length of telomeres. For example, the anti-aging composition can be combined with at least one pharmaceutically acceptable carrier to form a pharmaceutical composition. Among them, the anti-aging composition can be prepared in any convenient dosage form, such as tablets, capsules, powders, granules, or liquids.

[0027] The anti-aging composition can be administered to the required individual through various suitable administration routes. For example, the anti-aging composition can be administered to the required individual orally or parenterally. For example, the anti-aging composition can be administered to the required individual by intravenous injection (abbreviated as IV injection), intramuscular injection (abbreviated as IM injection), intraperitoneal injection (abbreviated as IP injection), transdermal absorption, sublingual absorption, or inhalation.

[0028] In addition, the anti-aging composition can be administered to the mammalian individual at a dosage of approximately 40 to 80 mg / kg / day. For example, for a mammalian individual weighing 75 kg (such as an adult male), 4 to 6 grams of the anti-aging composition can be administered per day, and it can be continuously administered for 2 months; while for a mammalian individual weighing 25 kg (such as a medium-sized dog), 1 to 2 grams of the anti-aging composition can be administered per day, and it can be continuously administered for 1 month. However, the aforementioned dosage should vary according to the differences in the mammalian individual and the administration route, which will not be restricted herein.

[0029] To prove that the anti-aging composition is indeed helpful in extending the telomere length of mammalian individuals, the following experiments were conducted:

[0030] (A) Results of human trials

[0031] In this experiment, 20 subjects aged between 28 and 67 years were recruited. Before the experiment, oral mucosal cells were taken from them, and the telomere length of the oral mucosal cells was analyzed. After orally administering the anti-aging composition for 2 months, the telomere length of the oral mucosal cells of each subject was analyzed again. The comparison results are shown in Table 1.

[0032] Table 1. Changes in telomere length and corresponding age changes of each subject in this experiment

[0033]

[0034]

[0035] Please refer to Table 1. The average telomere length of all subjects was extended by 1.074 Kb, indicating that the anti-aging composition is indeed helpful in extending the telomere length, thereby achieving the anti-aging effect.

[0036] (B) Results of animal trials

[0037] In this experiment, 9 medium-sized dogs of breeds including Labrador, German Shepherd, and Giant Schnauzer were recruited, and their ages were distributed between 1 and 7 years. Before the experiment, oral mucosal cells were taken from them, and the telomere length of the oral mucosal cells was analyzed. After orally administering the anti-aging composition for 1 month, the telomere length of the oral mucosal cells of each test dog was analyzed again. The comparison results are shown in Table 2.

[0038] Table 2. Changes in telomere length of each subject in this experiment

[0039]

[0040]

[0041] Refer to Table 2. The average telomere length of all test dogs extended from 14.25 Kb to 18.34 Kb, with an average extension of 4.09 Kb (paired sample t-test showed that P < 0.05, indicating significant statistical significance), showing that not only in human individuals, but also in other mammalian individuals, the administration of this anti-aging composition can extend the telomere length.

[0042] In summary, for the anti-aging composition of the present invention, the mitochondrial activity of the mammalian individual is activated by the salvia extract to regulate the energy generation of cells, so as to ensure sufficient energy for DNA repair and telomere maintenance. And due to the activity of the α-amylase inhibitor, the starch ingested by the mammalian individual is inhibited from being decomposed into glucose, thus reducing the absorption of sugars. The probiotic can adjust the microbial flora of the digestive tract of the mammalian individual. Through the synergistic effect of the foregoing components, the telomere length can be extended together, and thus the anti-aging effect can be achieved.

[0043] Furthermore, for the use of this anti-aging composition, through the synergistic use of the salvia extract, the α-amylase inhibitor and the probiotic, whether through oral mucosa sampling or blood sample analysis, it can be observed that it can effectively extend the telomere length, thereby delaying the aging of mammalian individuals, which is the efficacy of the present invention.

[0044] Although the present invention has been disclosed by the above preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make various changes and modifications to the above embodiments without departing from the spirit and scope of the present invention, and still fall within the technical scope protected by the present invention. Therefore, the protection scope of the present invention should include the meaning recorded in the appended patent application scope and all changes within the equivalent scope.

Claims

1. An anti-aging composition, characterized in that include: The composition comprises 5-20% of a salvia miltiorrhiza extract, 25-40% of an α-amylase inhibitor and 50-60% of a probiotic, wherein the probiotic comprises an animal bifidobacterium and a rhamnosus lactobacillus, and the total number of the animal bifidobacterium and the rhamnosus lactobacillus in each gram of the anti-aging composition is 1×10 10 CFU.

2. The anti-aging composition according to claim 1, characterized in that In each gram of the anti-aging composition, the bacterial counts of the animal bifidobacterium and the rhamnosus lactobacillus are respectively between 2×10 9 ~8×10 9 Between CFU.

3. The anti-aging composition according to claim 1 or 2, characterized in that: The salvia miltiorrhiza extract is obtained by extracting a root sample of salvia miltiorrhiza with water at a temperature of 70-100°C.

4. The anti-aging composition according to claim 1 or 2, wherein include: The extract is 1-10% by weight, and the extract is selected from at least one of an alfalfa extract, a bitter melon extract, a black plum extract, a strawberry extract, a rhodiola rosea extract, a chrysanthemum extract and a mulberry leaf extract.

5. The use of the anti-aging composition according to any one of claims 1 to 4, characterized in that: The invention is used for preparing anti-aging medicine, wherein the anti-aging composition is administered to a mammalian individual.

6. The use of the anti-aging composition according to claim 5, characterized in that: The anti-aging composition is administered orally to the mammalian subject.

7. The use of the anti-aging composition according to claim 5 or 6, characterized in that: The mammalian individual is a human individual.

8. The use of the anti-aging composition according to claim 7, characterized in that: For a mammalian individual with a body weight of 75 kg, the anti-aging composition is administered to the mammalian individual at a dosage of 4 to 6 grams per day for two consecutive months.

9. The use of the anti-aging composition according to claim 5 or 6, characterized in that: The mammalian individual is a dog individual or a cat individual.

10. The use of the anti-aging composition according to claim 9, characterized in that: For a mammalian individual with a body weight of 25 kg, the anti-aging composition is administered to the mammalian individual at a dosage of 1 to 2 grams per day for a continuous period of 1 month.

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