Traditional Chinese medicine composition for preventing and treating osteoporosis and preparation method thereof

Abstract

The invention belongs to the technical field of traditional Chinese medicine preparations, and particularly relates to a traditional Chinese medicine composition for preventing and treating osteoporosis and a preparation method thereof. The traditional Chinese medicine composition provided by the invention is prepared from the following raw materials in parts by weight: 15 to 30 parts of radix rehmanniae praeparata, 10 to 20 parts of rhizoma drynariae, 9 to 15 parts of herba epimedii, 10 to 15 parts of cortex eucommiae, 10 to 15 parts of rhizoma atractylodis macrocephalae, 9 to 12 parts of poria cocos, 9 to 15 parts of radix angelicae sinensis, 6 to 12 parts of radix achyranthis bidentatae, 5 to 10 parts of tortoise-shell glue, 10 to 20 parts of aizoon stonecrop herb and 0.001 to 0.03 part of vitamin K2. The tortoise-shell glue (for nourishing yin and stopping bleeding), the aizoon stonecrop herb (for dispersing blood stasis, promoting tissue regeneration and increasing calcium element), the bighead atractylodes rhizome and the poria cocos are introduced to form a three-dimensional regulating system of tonifying kidney, strengthening spleen and activating blood; vitamin K2 activates osteocalcin gamma-carboxylation, and forms two-way regulation of promoting bone formation and inhibiting bone resorption with traditional Chinese medicines. The bone mineral density is obviously improved, the fracture risk is reduced, and the composition is also suitable for preventing and treating primary and secondary osteoporosis and the like.

Description

Technical Field

[0001] The present invention belongs to the technical field of traditional Chinese medicine preparations, and particularly relates to a traditional Chinese medicine composition for preventing and treating osteoporosis and a preparation method thereof. The present invention introduces tortoise shell glue (nourishing yin and stopping bleeding), sedum aizoon (promoting blood circulation to remove stasis and increasing calcium element), atractylodes macrocephala, and poria cocos to form a three-dimensional regulation system of "tonifying the kidney - strengthening the spleen - promoting blood circulation". Vitamin K2 activates the γ-carboxylation of osteocalcin and forms a two-way regulation with traditional Chinese medicine of "promoting bone formation + inhibiting bone resorption". It can significantly increase bone density and reduce the risk of fracture, and is also applicable to preventing and treating primary and secondary osteoporosis, etc. Background Art

[0002] Osteoporosis belongs to the category of "bone flaccidity" in traditional Chinese medicine. Traditional treatments mostly use the combination of calcium agents and vitamin D, but there are defects such as low absorption rate and easy induction of vascular calcification. The treatment of osteoporosis with traditional Chinese medicine compound mostly focuses on tonifying the kidney and strengthening bones. Existing traditional Chinese medicine compounds mostly emphasize single kidney tonifying or spleen strengthening, and insufficiently consider the compound pathogenesis of "kidney deficiency and blood stasis". There are the following limitations: single target effect: Although traditional kidney-tonifying traditional Chinese medicines (such as epimedium brevicornu and eucommia ulmoides) can promote bone formation, they have insufficient inhibition of bone resorption; metabolic imbalance: lack of systematic regulation of calcium and phosphorus metabolism and rely on exogenous calcium agents; insufficient symptom relief: weak pertinence to low back pain and fracture healing. Summary of the Invention

[0003] The purpose of the present invention is to provide a traditional Chinese medicine composition for preventing and treating osteoporosis and a preparation method thereof, which condition and prevent and treat osteoporosis based on the synergistic effect of the traditional Chinese medicine method of tonifying the kidney, strengthening the spleen, and promoting blood circulation and vitamin K2.

[0004] The technical solution of the present invention is as follows: Firstly, a traditional Chinese medicine composition for preventing and treating osteoporosis is provided, which is characterized by comprising the following components in parts by weight: 15 - 30 parts of rehmannia glutinosa preparata, 10 - 20 parts of drynaria rhizome, 9 - 15 parts of epimedium brevicornu, 10 - 15 parts of eucommia ulmoides, 10 - 15 parts of atractylodes macrocephala, 9 - 12 parts of poria cocos, 9 - 15 parts of angelica sinensis, 6 - 12 parts of achyranthes bidentata, 5 - 10 parts of tortoise shell glue, 10 - 20 parts of sedum aizoon, and 0.001 - 0.03 parts of vitamin K2.

[0005] Here, rehmannia glutinosa preparata, drynaria rhizome, and epimedium brevicornu are commonly used medicinal materials in traditional Chinese medicine, with rich traditional efficacy and modern pharmacological support. For example, Traditional effects of Rehmannia glutinosa: nourishing blood and yin, replenishing essence and marrow, relieving umbilical and abdominal pain, promoting blood circulation, tonifying spleen yin, resolving phlegm, promoting qi flow, inducing sweating, restoring yang, etc. Modern pharmacology shows that: Immune system: Rehmannia glutinosa has a regulatory effect on the immune system and can enhance the body's immune function. Hematological system: Rehmannia glutinosa has a significant impact on the hematological system and can improve blood routine indicators. Nervous system: Rehmannia glutinosa also has a certain regulatory effect on the nervous system and can improve nerve function. Anti-aging: Rehmannia glutinosa has anti-aging effects and can delay cell aging. In the present invention, the main active components of Rehmannia glutinosa, catalpol and rehmannioside D, promote bone formation by upregulating the activity of alkaline phosphatase (ALP) in osteoblasts. In combination with Epimedium brevicornum, it regulates osteoblast differentiation through estrogen signaling pathway and thyroid hormone pathway, and significantly increases bone mineral density (BMD) and bone volume fraction.

[0006] Traditional effects of Drynaria fortunei: treating injuries and relieving pain, tonifying the kidney and strengthening bones, dispelling wind and removing freckles. Modern pharmacology shows that: Anti-osteoporosis: Drynaria fortunei has significant anti-osteoporosis effects, can promote bone formation and inhibit bone resorption. Fracture healing: Drynaria fortunei can promote fracture healing and accelerate bone injury repair. Kidney protection: Drynaria fortunei has a protective effect on the kidneys and can prevent and treat kidney injury. Anti-inflammatory: Drynaria fortunei has anti-inflammatory effects and can reduce inflammatory responses. Antioxidant: Drynaria fortunei has antioxidant effects, can scavenge free radicals and delay cell aging. Hypolipidemic: Drynaria fortunei can reduce blood lipid levels and prevent cardiovascular diseases. In the present invention, the total flavonoids in Drynaria fortunei promote the proliferation and differentiation of osteoblasts by regulating signaling pathways such as Wnt / β-catenin, MAPK, OPG / RANKL / RANK. Inhibit the Notch signaling pathway, reduce inflammatory factors (TNF-α, IL-6), and improve bone metabolic imbalance.

[0007] Traditional effects of Epimedium brevicornum: tonifying kidney yang, strengthening tendons and bones, dispelling wind and dampness. Modern pharmacology shows that: Cardiovascular and cerebrovascular: Epimedium brevicornum can increase blood flow in the cardiovascular and cerebrovascular systems and improve blood circulation. Reproductive system: Epimedium brevicornum can improve the function of the reproductive system, promote the secretion of sex hormones and improve sexual function. Immune regulation: Epimedium brevicornum has immune regulatory effects and can enhance the body's immunity. Antioxidant: Epimedium brevicornum has antioxidant effects and can delay cell aging. Anti-aging: Epimedium brevicornum has anti-aging effects and can delay skin aging. In the present invention, icariin in Epimedium brevicornum promotes the proliferation of osteoblasts through the BMP-2 / Smad4 pathway and upregulates the expression of osteocalcin (OCN). Regulates estrogen metabolism, increases serum estradiol levels, and delays bone loss.

[0008] In the present invention, the main functions of Eucommia ulmoides are to tonify the liver and kidneys, strengthen tendons and bones, and promote collagen synthesis. The ethanol extract in Eucommia ulmoides promotes the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) through the RhoA / ROCK signaling pathway, and up-regulates the expression of Runx2 and OPN. The leaf extract increases the secretion of osteocalcin by osteoblasts in a concentration-dependent manner and has significant proliferation.

[0009] Atractylodes macrocephala invigorates the spleen and eliminates dampness, and improves calcium absorption rate. Poria cocos promotes diuresis and percolates dampness, and regulates the intestinal flora to promote the endogenous synthesis of vitamin K2. Here, the combination of Atractylodes macrocephala and Poria cocos plays a synergistic mechanism by anti-inflammatory and regulating the intestinal flora metabolism to improve the bone microenvironment.

[0010] Angelica sinensis nourishes blood and promotes blood circulation, and repairs bones. The main function of Achyranthes bidentata is to direct the drug downward and target the lower limb bones. Here, achyranthes saponins enhance the mineralization ability of osteoblasts through the PI3K / Akt pathway and have a significant effect on preventing and treating femoral head defects.

[0011] Tortoise shell glue is rich in collagen and calcium ions. Its fat-soluble components can promote bone matrix mineralization and the migration of osteoblasts. Sedum aizoon disperses stasis and supplements calcium elements, and reduces the release of inflammatory factors after fracture. Its main functions are calcium supplementation, antioxidant and bone metabolism regulation. Sitosterol and salidroside in Sedum aizoon enhance bone density through antioxidant and calcium metabolism regulation.

[0012] Here, it should be added that Sedum aizoon, also known as Sedum aizoon L. and Sedum kamtschaticum Fisch., is rich in active ingredients such as calcium, flavonoids, polysaccharides, and organic acids. The calcium content can reach 300-600 mg / 100g fresh weight, and it is a typical "high-calcium plant". It has been traditionally used for promoting blood circulation to remove blood stasis and strengthening tendons and bones. Modern research shows that it has the effects of calcium supplementation, antioxidant and bone metabolism regulation. In the present invention, Sedum aizoon is combined with vitamin K2: Sedum aizoon provides a calcium source, and vitamin K2 carboxylates osteocalcin (OC), which can improve the efficiency of bone calcium deposition. Sedum aizoon is compatible with Drynaria fortunei and Eucommia ulmoides: they synergistically activate the Wnt pathway and promote the osteogenic differentiation of BMSCs.

[0013] The mechanism of action of vitamin K2 in bone metabolism is mainly achieved through the following aspects: Activating the SXR receptor: Vitamin K2 promotes the transcription of genes related to the extracellular matrix of bone cells and the accumulation of collagen by activating the SXR receptor. It helps to enhance bone formation and reduce bone resorption. Promoting the expression of osteocalcin and matrix Gla protein: Vitamin K2 can promote the expression of vitamin K-dependent proteins such as osteocalcin and matrix Gla protein. These proteins play a key role in bone formation and maintenance. Inhibiting osteoclast activity: Vitamin K2 can inhibit the differentiation and function of osteoclasts, thereby reducing bone resorption. It helps to maintain bone density and prevent osteoporosis. Promoting osteoblast generation: Vitamin K2 promotes the generation and differentiation of osteoblasts through multiple pathways, including autophagy induction and γ-carboxylation-dependent mechanisms. It helps to enhance bone formation. Regulating calcium metabolism: Vitamin K2 promotes the deposition of calcium in bones by regulating calcium metabolism, thereby enhancing bone density and strength.

[0014] Here, vitamin K2 enhances calcium deposition by carboxylating osteocalcin (OC), and synergistically promotes autophagy with traditional Chinese medicine components (such as calcium elements and icariin contained in Sedum aizoon), improving the differentiation efficiency of osteoblasts. In particular, the synergistic effect of vitamin K2 and the high calcium elements in Sedum aizoon further enhances its benefits for bone health. Vitamin K2 can promote the synthesis of osteocalcin induced by 1,25-dihydroxyvitamin D3 and increase the accumulation of osteocalcin in the extracellular matrix of bone cells.

[0015] The present invention also provides a preparation method of a traditional Chinese medicine composition for preventing and treating osteoporosis, which is characterized in that: the prepared medicinal material raw materials in the above ratio are first put into a heating container containing 700-3000 parts by weight of hydrogen-rich water and soaked for 0.5-2 hours, and then decocted for 15-30 minutes, and the medicinal liquid is filtered out, which is the traditional Chinese medicine preparation of the traditional Chinese medicine composition for preventing and treating osteoporosis.

[0016] Here, the prepared traditional Chinese medicine raw material (it should be noted that vitamin K2 is not included here) is first pulverized, and then put into a heating container containing 700-2000 parts by weight of hydrogen-rich water and soaked for 0.5-2 hours. It is preferably a heating container with good airtightness to reduce the escape of molecular hydrogen, and then decocted for 15-30 minutes, and the medicinal liquid is filtered out. Then, the prepared vitamin K2 is put into the filtered medicinal liquid and stirred evenly, which is the traditional Chinese medicine preparation of the traditional Chinese medicine composition for preventing and treating osteoporosis. It should be added that vitamin K2 can also be first included by β-cyclodextrin and then added to the above-filtered medicinal liquid, mainly for improving the stability of vitamin K2. The hydrogen content of the hydrogen-rich water here is at least 1000 ppb.

[0017] It should be noted that the benefits of hydrogen-rich water in the process of soaking and decocting Chinese herbal medicines or taking medicinal liquids are mainly based on the biomedical properties of hydrogen and the improvement of traditional decocting techniques: First, the physical and chemical properties of molecular hydrogen and the enhancement of decoction efficiency, including: 1. Protecting heat-sensitive components. High temperatures during the decocting process may damage the heat-sensitive active components in Chinese herbal medicines. As a strong reducing agent, hydrogen may reduce the damage to effective components caused by oxidative stress by neutralizing free radicals (such as hydroxyl radicals) generated during decoction. 2. Promoting the dissolution of fat-soluble components. Hydrogen is fat-soluble and can penetrate into the cell membranes of medicinal materials, destroying the lipid structure and increasing the cell membrane permeability, thereby promoting the release of fat-soluble components (such as flavonoids and alkaloids). 3. Regulating the redox state of the decoction liquid. Metal ions (such as iron and copper) may catalyze oxidation reactions during the decocting process, while hydrogen can maintain a reducing microenvironment in the decoction liquid and reduce component transformation.

[0018] Second, the potential for synergistic efficacy, such as: 1. Enhancing antioxidant and anti-inflammatory activities. Hydrogen and antioxidant components in Chinese herbal medicines (such as polyphenols and polysaccharides) may synergistically scavenge free radicals to enhance the overall antioxidant capacity. 2. Improving drug absorption and bioavailability. Hydrogen can regulate the intestinal flora and intestinal mucosal permeability. If the decocted medicinal liquid is used orally, it may promote the absorption of effective components.

[0019] Third, the safety of the process: 1. Reducing the risk of dissolution of toxic components. Some toxic Chinese herbal medicines need to strictly control the decocting time during decoction to reduce the dissolution of toxic components. Hydrogen may accelerate the decomposition of toxic components through reduction. 2. Inhibiting microbial contamination. Hydrogen has antibacterial effects and can reduce the growth of microorganisms during the storage of the decocted medicinal liquid, which is especially valuable for tonic herbs that require long decoction.

[0020] The positive effects of the present invention are: By conditioning based on the synergistic effect of the traditional Chinese medicine method of tonifying the kidney, strengthening the spleen, and promoting blood circulation to prevent and treat osteoporosis and other problems: In terms of compatibility, tortoise shell glue (nourishing yin and stopping bleeding), sedum aizoon (promoting blood circulation to remove stasis and calcium supplementation), and atractylodes macrocephala and poria cocos (strengthening the spleen and promoting diuresis) are introduced to form a three-dimensional regulation system of "tonifying the kidney - strengthening the spleen - promoting blood circulation"; In terms of component synergy: Adding vitamin K2 activates the γ-carboxylation of osteocalcin and forms a two-way regulation of "promoting bone formation + inhibiting bone resorption" with traditional Chinese medicine.

[0021] In summary, the prepared rehmannia root and drynaria rhizome in this formula are used to tonify the kidney and replenish essence; epimedium and eucommia ulmoides are used to strengthen tendons and bones; atractylodes macrocephala and poria cocos strengthen the spleen and promote digestion to assist absorption; angelica sinensis and sedum aizoon promote blood circulation to improve bone microcirculation; vitamin K2 synergistically promotes the carboxylation of osteocalcin with tortoise shell glue and sedum aizoon, forming a multi-dimensional anti-osteoporosis effect. The role of vitamin K2 in bone metabolism is mainly achieved by activating the SXR receptor, promoting the expression of osteocalcin and matrix Gla protein, inhibiting osteoclast activity, promoting osteoblast generation, and regulating calcium metabolism. It helps to maintain bone health and prevent osteoporosis and other bone diseases. Detailed implementation manners

[0022] In order to make the objectives, technical solutions and advantages of the present invention clearer and more understandable, the present invention is described by the following specific embodiments, but is by no means limited thereto. The following are the preferred embodiments of the present invention, which are only used to describe the present invention and should not be construed as a limitation of the present invention. It should be noted that any modifications, equivalent replacements and improvements made within the spirit and principle of the present invention shall be included within the protection scope of the present invention. Embodiment

[0023] 25 g of Rehmannia glutinosa, 15 g of Drynaria fortunei, 12 g of Epimedium brevicornu, 12 g of Eucommia ulmoides, 12 g of Atractylodes macrocephala, 10 g of Poria cocos, 12 g of Angelica sinensis, 9 g of Achyranthes bidentata, 8 g of tortoise shell glue, 15 g of Sedum aizoon. First, the above-prepared Chinese medicinal material raw materials are pulverized, then placed in a casserole containing 1800 g of hydrogen-rich water and soaked for 2 hours, then decocted for 30 minutes, the medicinal liquid is filtered out, and then 0.02 g of prepared vitamin K2 is put into the filtered medicinal liquid and stirred evenly to obtain a traditional Chinese medicine preparation for adjuvant treatment of amyotrophic lateral sclerosis.

[0024] Embodiment 2: 15 g of Rehmannia glutinosa, 10 g of Drynaria fortunei, 9 g of Epimedium brevicornu, 10 g of Eucommia ulmoides, 10 g of Atractylodes macrocephala, 9 g of Poria cocos, 9 g of Angelica sinensis, 6 g of Achyranthes bidentata, 5 g of tortoise shell glue, 10 g of Sedum aizoon. First, the above-prepared Chinese medicinal material raw materials are pulverized, then placed in a casserole containing 700 g of hydrogen-rich water and soaked for 0.5 hour, then decocted for 15 minutes, the medicinal liquid is filtered out, and then 0.001 g of prepared vitamin K2 is put into the filtered medicinal liquid and stirred evenly to obtain a traditional Chinese medicine preparation for adjuvant treatment of amyotrophic lateral sclerosis.

[0025] Embodiment 3: 20 g of Rehmannia glutinosa, 18 g of Drynaria fortunei, 13 g of Epimedium brevicornu, 13 g of Eucommia ulmoides, 14 g of Atractylodes macrocephala, 11 g of Poria cocos, 13 g of Angelica sinensis, 11 g of Achyranthes bidentata, 8 g of tortoise shell glue, 18 g of Sedum aizoon. First, the above-prepared Chinese medicinal material raw materials are pulverized, then placed in a casserole containing 1200 g of hydrogen-rich water and soaked for 1 hour, then decocted for 20 minutes, the medicinal liquid is filtered out, and then 0.01 g of prepared vitamin K2 is put into the filtered medicinal liquid and stirred evenly to obtain a traditional Chinese medicine preparation for adjuvant treatment of amyotrophic lateral sclerosis.

[0026] Embodiment 4: 30 g of Rehmannia glutinosa, 20 g of Drynaria fortunei, 15 g of Epimedium brevicornu, 15 g of Eucommia ulmoides, 15 g of Atractylodes macrocephala, 12 g of Poria cocos, 15 g of Angelica sinensis, 12 g of Achyranthes bidentata, 10 g of tortoise shell glue, 20 g of Sedum aizoon. First, the above-prepared Chinese medicinal material raw materials are pulverized, then placed in a casserole containing 2000 g of hydrogen-rich water and soaked for 2 hours, then decocted for 30 minutes, the medicinal liquid is filtered out, and then 0.03 g of prepared vitamin K2 is put into the filtered medicinal liquid and stirred evenly to obtain a traditional Chinese medicine preparation for adjuvant treatment of amyotrophic lateral sclerosis.

[0027] Example 5: 23 grams of Rehmannia glutinosa Libosch. preparata, 16 grams of Drynaria fortunei (Kunze) J. Sm., 12 grams of Epimedium brevicornu Maxim., 12 grams of Eucommia ulmoides Oliv., 12 grams of Atractylodes macrocephala Koidz., 10 grams of Poria cocos (Schw.) Wolf, 10 grams of Angelica sinensis (Oliv.) Diels, 9 grams of Achyranthes bidentata Blume, 9 grams of Colla carapacis et plastri Testudinis, 13 grams of Sedum aizoon L. First, the above-prepared traditional Chinese medicine raw materials are crushed, then soaked in a casserole containing 1500 grams of hydrogen-rich water for 1 hour, and then decocted for 20 minutes. The medicinal liquid is filtered out, and then 0.006 grams of vitamin K2 prepared in proportion is put into the filtered medicinal liquid and stirred evenly to obtain a traditional Chinese medicine preparation for adjuvant treatment of amyotrophic lateral sclerosis.

[0028] The following are experimental cases, and the verification is carried out on mice based on the traditional Chinese medicine preparation of the formula in Example 1.

[0029] I. Experimental design 1. Selection of animal model An ovariectomized female mouse model is used to simulate postmenopausal osteoporosis, because it can significantly reduce bone mineral density and enhance bone resorption activity. A low-calcium diet model can also be supplemented to observe the regulatory effect of the formula on calcium metabolism. 2. Grouping and dosing regimen Experimental group: The water decoction of the traditional Chinese medicine formula is administered by gavage (the dose is converted according to body weight, and the equivalent dose of mice is about 6-10 times that of the adult clinical dosage).

[0030] Control group: Normal control group (sham operation group) Model group (OVX or low-calcium diet, without intervention) Positive control group (such as bisphosphonate or vitamin D + calcium agent) Course of treatment: Continuous administration for 8-12 weeks to simulate the long-term conditioning effect of traditional Chinese medicine.

[0031] 3. Detection indexes Bone mineral density and bone biomechanics: The bone mineral density of the femur and lumbar vertebra is measured by dual-energy X-ray absorptiometry (DEXA) or solid density meter, and the bone strength is evaluated by combining the three-point bending test.

[0032] Bone metabolism markers: Serum bone gla protein (BALP) and amino-terminal propeptide of type I procollagen (PINP) reflect bone formation; tartrate-resistant acid phosphatase (TRACP-5b) reflects bone resorption.

[0033] Inflammatory factors: Detect the levels of IL-6 and TNF-α in bone tissue to evaluate the anti-inflammatory effect.

[0034] Histopathology: The number, thickness and separation degree of bone trabeculae are analyzed by HE staining or Micro-CT.

[0035] II. Experimental results 1. Improvement of bone mineral density and bone strength The bone mineral density of the femur and lumbar spine in the Chinese medicine group mice was significantly higher than that in the model group, the number of trabeculae increased, and the separation decreased.

[0036] 2. Bidirectional regulation of bone metabolism The levels of serum BALP and PINP increased (enhanced bone formation), and the level of TRACP-5b decreased (inhibited bone resorption), reflecting the dual effects of "promoting formation - inhibiting resorption".

[0037] 3. Synergy of anti - inflammation and anti - oxidation The expressions of IL - 6 and TNF - α in bone tissue decreased, and inflammatory bone destruction was reduced, which was related to the anti - inflammatory mechanisms of Epimedium brevicornu and Sedum aizoon.

[0038] In summary, although the embodiments of the present invention have been disclosed above, it is not limited to the applications listed in the specification and embodiments. It can be fully applied to various fields suitable for the present invention. For those familiar with the field, additional modifications can be easily achieved. Therefore, without departing from the general concept defined by the claims and the equivalent scope, the present invention is not limited to specific details.

Claims

1. A Chinese medicine composition for preventing and treating osteoporosis, characterized in that The invention comprises the following components in parts by weight: 15-30 parts of Rehmannia root, 10-20 parts of Drynaria fortunei, 9-15 parts of Epimedium, 10-15 parts of Eucommia ulmoides, 10-15 parts of Atractylodes macrocephala, 9-12 parts of Poria, 9-15 parts of Angelica sinensis, 6-12 parts of Achyranthes bidentata, 5-10 parts of Tortoise shell glue, 10-20 parts of Sedum multiflorum and 0.001-0.03 parts of Vitamin K2.

2. A method for preparing a Chinese medicine composition for preventing and treating osteoporosis, characterized in that: The above-proportioned raw materials are first placed in a heating container with 700-3000 parts by weight of hydrogen-rich water and soaked for 0.5-2 hours, then decocted for 15-30 minutes, and the medicinal liquid is filtered out to obtain the Chinese medicine preparation of the Chinese medicine composition for preventing and treating osteoporosis.