Mass microorganism culture medium

Abstract

The culture medium comprises an incubator, the top of the incubator is provided with a detection unit for detecting the number of microorganisms in the incubator, and the top of the incubator is further provided with a quantitative feeding mechanism for quantitatively feeding a culture solution; the invention relates to the technical field of microbial culture. According to the mass microorganism culture medium, through the arrangement of the quantitative feeding mechanism and the driving assembly, automatic quantitative feeding of a culture solution is achieved, the culture solution is quantitatively fed and discharged once by pressing a pressing cylinder once, meanwhile, a rotating plate rotates by 90 degrees, and the quantitatively fed culture solution falls into a culture box through a fan-shaped groove; along with culture and reproduction of microorganisms, quantitative feeding is carried out multiple times, the amount of the culture solution is increased, the concentration and the amount of the culture solution are synchronously increased when the microorganisms are gradually increased, quantitative feeding facilitates statistics of feeding times, and therefore the concentration and the amount of the culture solution are calculated, and precise culture of the microorganisms is achieved.

Description

Technical Field

[0001] The invention relates to the technical field of microbial culture, in particular to a large amount of microbial culture medium. Background Art

[0002] The use of microorganisms has greatly improved the quality of human life. Microorganisms are used in a wide range of areas. They are generally used to control pathogens, pests, weeds or induce crops, or are products made from the active ingredients of microorganisms through formulation. They are generally isolated from nature and can also be improved through artificial strains, such as artificial mutagenesis, selection or genetic modification.

[0003] The Chinese patent application with publication number CN220352092U discloses a microbial composite culture medium for microbial detection, comprising: a base cover, the base cover is hinged to the culture medium through a rotating shaft, and a first slot is welded to the surface of the base cover; a culture medium, a base is provided at the bottom of the culture medium; a slide seat, the slide seat is welded to the base, and the slide seat height is greater than the height of the culture medium; the beneficial effect is: the utility model proposes a culture medium with a base, a sealing ring, and a sealing groove, the sealing ring is in an inverted trapezoidal shape, and the sealing groove is trapezoidal, which can ensure that when the base cover is closed, the small mouth of the sealing ring faces the large mouth of the sealing groove, and then the buckle and the first slot are connected to the buckle to isolate the air, the operator only needs to buckle the buckle to open the base cover, and avoid liquid scattering caused by excessive force when twisting, the tough steel sheet can enter the slide groove with the slide seat, and when the slide seat slides to the top, the elastic tough sheet bounces to the inner wall of the second slot, and the tough steel sheet and the inner wall of the second slot are offset to play a fixing role. However, as the number of microorganisms gradually increases during cultivation, the concentration of the required culture solution also needs to be increased. The culture solution cannot be added automatically and quantitatively when it increases, which makes it inconvenient to statistically calculate the concentration of the culture solution. In addition, after the culture solution is added, it cannot evenly cover the surface of the microorganisms, which will make the microbial culture efficiency in different parts of the incubator different. In this regard, we have proposed a large amount of microbial culture medium to solve the above problems. Summary of the invention

[0004] In view of the deficiencies of the prior art, the present invention provides a large amount of microbial culture medium to solve the problems raised in the background technology.

[0005] To achieve the above object, the present invention is implemented by the following technical scheme: a large amount of microbial culture medium, including an incubator, the top of the incubator is provided with a detection unit for detecting the number of microorganisms in the incubator, and the top of the incubator is also provided with a quantitative delivery mechanism for quantitatively delivering culture solution;

[0006] The quantitative dispensing mechanism includes a fixed box fixed to the top of the incubator, a box cover is fixed on the top of the fixed box, a fan-shaped groove is penetrated through the bottom of the fixed box, a liquid hopper is connected to the top of the box cover, a cylinder is fixed on the top wall of the box cover, a pressing cylinder is slidably connected to the top of the box cover, the bottom of the pressing cylinder extends to the inside of the cylinder, a rotating cylinder is rotatably connected to the bottom wall of the cylinder, a connecting column is fixed to the bottom of the rotating cylinder, the bottom of the connecting column extends to the outside of the cylinder, four rotating plates are fixed on the outer surface of the connecting column, a wave groove is opened on the outer surface of the rotating cylinder, convex columns are fixed on both sides of the pressing cylinder, the opposite ends of the two convex columns extend to the inside of the wave groove, and the convex columns slide vertically in the wave groove, a spring is arranged between the top wall of the pressing cylinder and the inside of the rotating cylinder, a folding rod is fixed on one side of the pressing cylinder, a vertical rod is fixed on the bottom of the folding rod, and a blocking cone is fixed on the bottom end of the vertical rod.

[0007] Preferably, a driving assembly for driving the pressing cylinder to automatically press is provided on the top of the box cover, and the driving assembly includes two vertical plates fixed on the top of the box cover, and a rotating rod is rotatably connected between the opposite sides of the two vertical plates. A motor 1 is fixed to one side of the vertical plate, and the motor 1 drives the rotating rod to rotate. A cam is fixed on the rotating rod, and the outer surface of the cam is in contact with and squeezed with the top of the pressing cylinder.

[0008] Preferably, the detection unit includes a cylinder fixed on the top of the incubator, the bottom of the cylinder extends to the interior of the incubator, an air inlet is opened at the bottom of the cylinder, a lifting rod is slidably connected to the top of the cylinder, a piston is fixed to the bottom end of the lifting rod, the outer surface of the piston is slidably connected to the inner surface of the cylinder, and an electrode head is fixedly installed on the inner wall of the cylinder.

[0009] Preferably, the interior of the incubator is provided with a swing covering assembly for driving the culture fluid falling into the incubator to be evenly covered, the swing covering assembly includes a cross bar rotatably connected between the inner walls of the incubator, and a second motor is fixed to one side of the incubator, and the second motor drives the cross bar to rotate.

[0010] Preferably, a rotating wheel is fixed on the cross bar, a curved groove is provided on the outer surface of the rotating wheel, a connecting plate is fixed on one side of the inner wall of the incubator, the top of the connecting plate is rotatably connected to a rotating shaft, a swing rod is fixed to the top of the rotating shaft, a ball is fixed to one end of the swing rod, the ball slides inside the curved groove, and a triangular seat is fixed to the other end of the swing rod.

[0011] Preferably, diverter seats are fixed on both sides of the bottom of the triangular seat, and transverse grooves are provided inside the two diverter seats. A plurality of inclined grooves are also provided on the inclined surface of the diverter seat, and the inclined grooves are connected to the transverse grooves.

[0012] Preferably, a culture box is slidably connected to one side of the incubator, a microorganism tube is connected to one side of the incubator, a flow guide seat is fixed to one side of the inner wall of the incubator, a plurality of flow guide grooves are opened on the flow guide seat, and a connecting bucket is fixed between the inner walls of the incubator.

[0013] The present invention also discloses a method for detecting a large amount of microbial culture medium, which specifically comprises the following steps:

[0014] Step 1: inject the base liquid containing microorganisms into the culture box through the microorganism tube, the base liquid flows into the liquid receiving bucket, and slides along the inclined surface of the liquid receiving bucket to the guide seat, and is divided through the guide groove to allow the base liquid to flow evenly into the culture box;

[0015] Step 2, pour the culture solution into the liquid bucket, start the motor 1, so that the motor 1 drives the rotating rod and the cam to rotate, and then drives the cam to reciprocate and squeeze the pressing cylinder, the convex column on the pressing cylinder slides in the wave groove, and then drives the rotating cylinder to rotate 90 degrees, and squeezes the compression spring at the same time. During the downward movement of the pressing cylinder, the folding rod, the vertical rod, and the blocking cone move downward at the same time. At this time, after the blocking cone moves downward, part of the culture solution flows into the space between the two rotating plates. With the reset of the spring, the blocking cone resets at the same time and seals the liquid outlet of the pressing cylinder, thereby completing the quantitative feeding operation on one side. With the rotation of the rotating plate, the culture solution is brought to the fan-shaped groove and falls into the incubator;

[0016] Step 3: After the culture liquid falls on the triangular seat, it slides down along its inclined surface to both sides and falls into the diverter seat. After being collected in the horizontal groove, it is discharged through each inclined groove, so that the culture liquid is evenly diverted. The second motor is started, and the second motor drives the rotating wheel to rotate, thereby driving the ball to slide in the curved groove, and then driving the swing rod, the triangular seat, and the diverter seat to swing left and right around the rotating shaft. During the swinging process, the culture liquid can be evenly sprinkled at various places near and far in the culture box, so that the culture liquid evenly covers the microorganisms;

[0017] Step 4: After waiting for 2 hours of microbial culture, pull the lifting rod to drive the piston upward, and then the piston extracts a part of the air containing microorganisms in the incubator into the cylinder, and then the air containing microorganisms contacts the electrode head. As the number of microbial cells increases, the charge on the cell membrane and the difference in ion concentration inside and outside the cell will also contribute to the conductivity of the solution. At the same time, the number of ions produced by microbial metabolism also increases, thereby improving the conductivity. The conductivity is detected using the circuit of the electrode head. When the conductivity is high, it means that the number of microorganisms is large;

[0018] Step 5. After detecting an increase in the number of microorganisms, start the motor 1 again to perform another quantitative feeding operation of the culture solution to increase the concentration of the culture solution and ensure the normal culture of the microorganisms. At the same time, record the number of quantitative feedings, calculate the concentration change of the culture solution based on the number of feedings, and finally draw out the culture box to count the number of microorganisms.

[0019] Beneficial Effects

[0020] The present invention provides a large amount of microbial culture medium. Compared with the prior art, it has the following beneficial effects:

[0021] (1) The automatic quantitative feeding of the culture solution is realized by setting the quantitative feeding mechanism and the driving component. The culture solution is quantitatively fed once by pressing the pressing cylinder, and the rotating plate rotates 90 degrees at the same time, so that the quantitatively fed culture solution falls into the incubator through the fan-shaped groove. As the microorganisms are cultured and reproduced, quantitative feeding is performed multiple times to increase the amount of culture solution. When the number of microorganisms gradually increases, the concentration and amount of the culture solution are simultaneously increased. Quantitative feeding facilitates the counting of the number of feedings, thereby calculating the concentration and amount of the culture solution, thereby realizing accurate cultivation of microorganisms.

[0022] (2) By setting up the swinging covering component, the culture liquid falling into the incubator is quickly diverted, and at the same time, the swinging of the culture liquid is realized so that it evenly covers the surface of the microorganisms, making the microbial culture efficiency at various locations in the incubator consistent.

[0023] (3) Through the setting of the detection unit, the air containing microorganisms in the incubator is sucked into the cylinder, and then the air containing microorganisms contacts the electrode head. As the number of microbial cells increases, the charge on the cell membrane and the difference in ion concentration inside and outside the cell will also contribute to the conductivity of the solution. At the same time, the number of ions produced by microbial metabolism also increases, thereby improving the conductivity. The conductivity is detected using the circuit of the electrode head. When the conductivity is high, it means that the number of microorganisms is large. At this time, the concentration of the culture solution needs to be increased, and a quantitative addition operation can be performed to provide conditions for the rapid reproduction of microorganisms. The setting of the detection unit realizes real-time monitoring and detection of the content of microorganisms in the incubator. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a three-dimensional diagram of the external structure of the present invention;

[0025] Figure 2 is a cross-sectional view of an incubator of the present invention;

[0026] Figure 3 It is a cross-sectional view of the box cover and the liquid hopper of the present invention;

[0027] Figure 4 It is a cross-sectional view of the cylinder and the pressing cylinder of the present invention;

[0028] Figure 5 For the present invention Figure 4 A partial enlarged view of the middle A;

[0029] Figure 6 A three-dimensional diagram of a swing cover assembly of the present invention;

[0030] Figure 7 It is a local structural stereogram of the present invention;

[0031] Figure 8 It is a cross-sectional view of the cylinder of the present invention.

[0032] In the figure: 1, incubator; 2, detection unit; 3, quantitative delivery mechanism; 4, drive assembly; 5, swing cover assembly; 6, incubator; 7, microorganism tube; 8, flow guide seat; 9, flow guide groove; 10, receiving bucket; 21, cylinder; 22, air inlet; 23, lifting rod; 24, piston; 25, electrode head; 31, fixed box; 32, box cover; 33, fan-shaped groove; 34, liquid bucket; 35, cylinder; 36, pressing cylinder; 37, rotating cylinder; 38, connection Column; 39, rotating plate; 310, wave groove; 311, convex column; 312, spring; 313, folding rod; 314, vertical rod; 315, stop cone; 41, vertical plate; 42, rotating rod; 43, motor 1; 44, cam; 51, cross bar; 52, motor 2; 53, rotating wheel; 54, curved groove; 55, connecting plate; 56, rotating shaft; 57, rocker rod; 58, sphere; 59, triangular seat; 510, diverter seat; 511, cross groove; 512, inclined groove. DETAILED DESCRIPTION

[0033] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0034] The present invention provides three technical solutions, including the following embodiments:

[0035] Example 1: Please refer to Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 , Figure 7 A large amount of microbial culture medium includes an incubator 1, which is used to culture microorganisms and is made of heat-insulating materials. A temperature sensor, a heating element, and a cooling element are arranged inside the incubator. The temperature sensor monitors the temperature in the incubator 1 in real time, and controls the heating element or the cooling element to work according to a preset temperature value, so that the temperature in the incubator is maintained within a range suitable for the growth of microorganisms, such as 35°C-37°C, and the temperature accuracy can be controlled within ±0.5°C. A detection unit 2 for detecting the number of microorganisms in the incubator 1 is arranged on the top of the incubator 1, and a quantitative delivery mechanism 3 for quantitatively delivering culture solution is also arranged on the top of the incubator 1;

[0036] The quantitative delivery mechanism 3 includes a fixed box 31 fixed on the top of the incubator 1, a box cover 32 is fixed on the top of the fixed box 31, and a fan-shaped groove 33 is provided through the bottom of the fixed box 31. The setting of the fan-shaped groove 33 is convenient for the delivery and feeding of the culture solution. The top of the incubator 1 is also provided with a fan-shaped groove 33 of the same size, which is convenient for the culture solution to fall into the incubator 1. The top of the box cover 32 is connected to a liquid hopper 34, and the liquid hopper 34 contains culture solution. The culture solution is a mixed liquid containing nutrients such as carbon source, nitrogen source, water, inorganic salts and growth factors. The formula of the culture solution will be adjusted according to the type of microorganism to be cultured to meet the needs of microbial growth, reproduction and metabolism. A cylinder 35 is fixed on the top wall of the box cover 32, and a pressing cylinder 36 is slidably connected to the top of the box cover 32. The bottom of the pressing cylinder 36 extends to the inside of the cylinder 35. A rotating cylinder 37 is rotatably connected to the bottom wall of the cylinder 35. A connecting column 38 is fixed to the bottom of the rotating cylinder 37. The bottom of the connecting column 38 extends The outer surface of the connecting column 38 is fixed with four rotating plates 39, and the outer surface of the rotating cylinder 37 is provided with a wave groove 310. The two sides of the pressing cylinder 36 are fixed with protruding columns 311. The opposite ends of the two protruding columns 311 extend to the inside of the wave groove 310, and the protruding columns 311 slide vertically in the wave groove 310. The protruding columns 311 slide downward in the wave groove 310 to drive the rotating cylinder 37 to rotate 90 degrees. The top wall of the pressing cylinder 36 and the inside of the rotating cylinder 37 are connected. A spring 312 is arranged between the cylinder 36 and the spring 312 is used to reset the cylinder 36. A folding rod 313 is fixed on one side of the cylinder 36. A vertical rod 314 is fixed to the bottom of the folding rod 313. A blocking cone 315 is fixed to the bottom of the vertical rod 314. The blocking cone 315 is located at the bottom of the liquid bucket 34. The blocking cone 315 blocks the liquid outlet of the liquid bucket 34, and the culture solution does not flow out. When the culture solution moves downward, it flows out quantitatively. When the spring 312 is reset, it drives the blocking cone 315 to reset and seal the liquid outlet.

[0037] A driving assembly 4 for driving the pressing cylinder 36 to automatically press is provided on the top of the box cover 32. The driving assembly 4 includes two vertical plates 41 fixed on the top of the box cover 32. A rotating rod 42 is rotatably connected between the opposite sides of the two vertical plates 41. A motor 43 is fixed on one side of the vertical plate 41. The motor 43 is a stepping motor that can control the number of rotations of the output shaft. The motor 43 is controlled by an external switch and is electrically connected to an external power supply. The motor 43 drives the rotating rod 42 to rotate. The output end of the motor 43 is fixed to one end of the rotating rod 42 through a coupling. A cam 44 is fixed on the rotating rod 42. The outer surface of the cam 44 contacts and squeezes the top of the pressing cylinder 36. The output shaft of the motor 43 rotates one circle, driving the cam 44 to rotate one circle, driving the pressing cylinder 36 to press once, thereby realizing the quantitative delivery of the culture solution.

[0038] By setting up the quantitative delivery mechanism 3 and the driving component 4, the automatic quantitative delivery of the culture solution is realized. By pressing the pressing cylinder 36 once, the culture solution is quantitatively delivered once, and at the same time, the rotating plate 39 rotates 90 degrees, so that the quantitatively delivered culture solution falls into the incubator 1 through the fan-shaped groove 33. With the cultivation and reproduction of microorganisms, quantitative delivery is performed multiple times to increase the amount of culture solution. When the number of microorganisms gradually increases, the concentration and amount of the culture solution are simultaneously increased. Quantitative delivery is convenient for counting the number of delivery times, thereby calculating the concentration and amount of the culture solution, thereby realizing accurate cultivation of microorganisms.

[0039] A culture box 6 is slidably connected to one side of the incubator 1. The culture box 6 is in the shape of a drawer and can be pulled out for observation when microorganisms need to be observed. A microorganism tube 7 is connected to one side of the incubator 1. A base liquid containing microorganisms is injected into the incubator 1 from the microorganism tube 7. A guide seat 8 is fixed to one side of the inner wall of the incubator 1. A plurality of guide grooves 9 are provided on the guide seat 8. A receiving bucket 10 is fixed between the inner walls of the incubator 1. The base liquid containing microorganisms falls into the receiving bucket 10, slides down the inclined surface of the receiving bucket 10, and falls on the guide groove 9 for diversion, thereby introducing the base liquid into the incubator 6.

[0040] Example 2: Based on Example 1, see Figure 6 and Figure 8 As shown, the detection unit 2 includes a cylinder 21 fixed on the top of the incubator 1, the bottom of the cylinder 21 extends to the interior of the incubator 1, an air inlet 22 is provided at the bottom of the cylinder 21, and the air containing microorganisms in the incubator 1 enters the cylinder 21 from the air inlet 22, a lifting rod 23 is slidably connected to the top of the cylinder 21, a piston 24 is fixed to the bottom end of the lifting rod 23, the outer surface of the piston 24 is slidably connected to the inner surface of the cylinder 21, an electrode head 25 is fixedly installed on the inner wall of the cylinder 21, and the electrode head 25 is electrically connected to a sensitive conductivity meter outside.

[0041] Through the setting of the detection unit 2, the air containing microorganisms in the incubator 1 is sucked into the cylinder 21, and then the air containing microorganisms contacts the electrode head 25. As the number of microbial cells increases, the charge on the cell membrane and the difference in ion concentration inside and outside the cell will also contribute more to the conductivity of the solution. At the same time, the number of ions produced by microbial metabolism also increases, thereby improving the conductivity. The conductivity is detected using the circuit of the electrode head. When the conductivity is high, it means that the number of microorganisms is large. At this time, the concentration of the culture solution needs to be increased, and a quantitative addition operation can be performed to provide conditions for the rapid reproduction of microorganisms. The setting of the detection unit 2 realizes real-time monitoring and detection of the content of microorganisms in the incubator 1.

[0042] The interior of the incubator 1 is provided with a swing covering assembly 5 for driving the culture solution falling into the incubator 1 to be evenly covered. The swing covering assembly 5 includes a cross bar 51 rotatably connected between the inner walls of the incubator 1. A motor 2 52 is fixed to one side of the incubator 1. The motor 2 52 is controlled by an external switch and is electrically connected to an external power supply. The motor 2 52 drives the cross bar 51 to rotate, and the output end of the motor 2 52 is fixed to one end of the cross bar 51 through a coupling.

[0043] A rotating wheel 53 is fixed on the cross bar 51, and a curved groove 54 is formed on the outer surface of the rotating wheel 53. A connecting plate 55 is fixed to one side of the inner wall of the incubator 1. A rotating shaft 56 is rotatably connected to the top of the connecting plate 55. A swing rod 57 is fixed to the top of the rotating shaft 56. A ball 58 is fixed to one end of the swing rod 57. The ball 58 slides inside the curved groove 54. A triangular seat 59 is fixed to the other end of the swing rod 57. The setting of the triangular seat 59 can make the culture solution flow to both sides along its inclined surface.

[0044] A diverter seat 510 is fixed on both sides of the bottom of the triangular seat 59. A transverse groove 511 is provided inside the two diverter seats 510. A plurality of inclined grooves 512 are provided on the inclined surface of the diverter seat 510. The inclined grooves 512 are connected to the transverse grooves 511. The transverse grooves 511 can store a small amount of culture fluid. When the culture fluid in the transverse grooves 511 is full, the culture fluid overflows and is evenly diverted and discharged through the inclined grooves 512.

[0045] By setting the swing covering component 5, the culture liquid falling into the incubator 1 is quickly diverted, and the swinging of the culture liquid is realized so that it evenly covers the surface of the microorganisms, making the microorganism culture efficiency in various parts of the incubator consistent.

[0046] Example 3: Based on Example 2, see Figure 1-Figure 8 As shown, the present invention also discloses a method for detecting a large amount of microbial culture medium, which specifically comprises the following steps:

[0047] Step 1: inject the base liquid containing microorganisms into the culture box 1 through the microorganism tube 7, the base liquid flows into the liquid receiving bucket 34, and slides along the inclined surface of the liquid receiving bucket 34 to the guide seat 8, and is divided by the guide groove 9, so that the base liquid flows evenly into the culture box 6;

[0048] Step 2, pour the culture solution into the liquid bucket 34, start the motor 1 43, so that the motor 1 43 drives the rotating rod 42 and the cam 44 to rotate, and then drives the cam 44 to reciprocate and squeeze the pressing cylinder 36, and the convex column 311 on the pressing cylinder 36 slides in the wave groove 310, and then drives the rotating cylinder 37 to rotate 90 degrees, and squeezes the compression spring 312 at the same time. When the pressing cylinder 36 moves downward, it drives the folding rod 313, the vertical rod 314, and the blocking cone 315 to move downward at the same time. At this time, after the blocking cone 315 moves downward, part of the culture solution flows into the space between the two rotating plates 39. With the reset of the spring 312, the blocking cone 315 is reset at the same time and seals the liquid outlet of the pressing cylinder 36, thereby completing the quantitative feeding operation on one side. With the rotation of the rotating plate 39, the culture solution is brought to the fan-shaped groove 33 and falls into the incubator 1;

[0049] Step 3: After the culture liquid falls onto the triangular seat 59, it slides down along its inclined surface to both sides and falls into the diverter seat 510. After being filled in the transverse groove 511, it is discharged through each inclined groove, so that the culture liquid is evenly diverted. The motor 2 52 is started, and the motor 2 52 drives the rotating wheel 53 to rotate, thereby driving the ball 58 to slide in the curved groove 54, and then driving the swing rod 57, the triangular seat 59, and the diverter seat 510 to swing left and right around the rotating shaft 56. During the swinging process, the culture liquid can be evenly sprinkled at various places near and far of the culture box 6, so that the culture liquid evenly covers the microorganisms;

[0050] Step 4: After waiting for 2 hours of microbial culture, pull the lifting rod 23 to make the lifting rod 23 drive the piston 24 to move upward, and then the piston 24 extracts a part of the air containing microorganisms in the incubator 1 into the cylinder 21, and then the air containing microorganisms contacts the electrode head 25. As the number of microbial cells increases, the charge on the cell membrane and the difference in ion concentration inside and outside the cell will also contribute to the conductivity of the solution. At the same time, the number of ions produced by microbial metabolism also increases, thereby improving the conductivity. The conductivity is detected using the circuit of the electrode head 25. When the conductivity is high, it means that the number of microorganisms is large.

[0051] Step 5: After detecting an increase in the number of microorganisms, start the motor 43 again to perform another quantitative feeding operation of the culture solution to increase the concentration of the culture solution and ensure the normal culture of the microorganisms. At the same time, record the number of quantitative feedings, calculate the concentration change of the culture solution based on the number of feedings, and finally draw out the culture box 6 to count the number of microorganisms.

[0052] Meanwhile, the contents not described in detail in this specification belong to the prior art known to those skilled in the art.

[0053] The above embodiments of the invention are described in detail, but the contents are only preferred embodiments of the invention and cannot be considered to limit the scope of the invention. All equivalent changes and improvements made within the scope of the invention should still fall within the scope of the invention.

Claims

1. A large amount of microbial culture medium, comprising an incubator (1), characterized in that: The top of the incubator (1) is provided with a detection unit (2) for detecting the number of microorganisms in the incubator (1), and the top of the incubator (1) is also provided with a quantitative delivery mechanism (3) for quantitatively delivering culture solution; The quantitative dispensing mechanism (3) comprises a fixed box (31) fixed on the top of the incubator (1); a box cover (32) is fixed on the top of the fixed box (31); a fan-shaped groove (33) is formed through the bottom of the fixed box (31); the top of the box cover (32) is connected to a liquid hopper (34); a cylinder (35) is fixed on the top wall of the box cover (32); a pressing cylinder (36) is slidably connected to the top of the box cover (32); the bottom of the pressing cylinder (36) extends to the inside of the cylinder (35); a rotating cylinder (37) is rotatably connected to the bottom wall of the cylinder (35); a connecting column (38) is fixed on the bottom of the rotating cylinder (37); the bottom of the connecting column (38) extends to the inside of the cylinder (35); 5), four rotating plates (39) are fixed on the outer surface of the connecting column (38), a wave groove (310) is opened on the outer surface of the rotating cylinder (37), convex columns (311) are fixed on both sides of the pressing cylinder (36), the opposite ends of the two convex columns (311) extend to the inside of the wave groove (310), and the convex columns (311) slide vertically in the wave groove (310), a spring (312) is arranged between the top wall of the pressing cylinder (36) and the inside of the rotating cylinder (37), a folding rod (313) is fixed on one side of the pressing cylinder (36), a vertical rod (314) is fixed at the bottom of the folding rod (313), and a blocking cone (315) is fixed at the bottom end of the vertical rod (314).

2. A large amount of microbial culture medium according to claim 1, characterized in that: A driving assembly (4) for driving the pressing cylinder (36) to automatically press is arranged at the top of the box cover (32), and the driving assembly (4) comprises two vertical plates (41) fixed on the top of the box cover (32), and a rotating rod (42) is rotatably connected between the opposite sides of the two vertical plates (41), and a motor (43) is fixed on one side of the vertical plate (41), and the motor (43) drives the rotating rod (42) to rotate, and a cam (44) is fixed on the rotating rod (42), and the outer surface of the cam (44) contacts and presses the top of the pressing cylinder (36).

3. A large amount of microbial culture medium according to claim 1, characterized in that: The detection unit (2) comprises a cylinder (21) fixed on the top of the incubator (1), the bottom of the cylinder (21) extends to the interior of the incubator (1), an air inlet (22) is provided at the bottom of the cylinder (21), a lifting rod (23) is slidably connected to the top of the cylinder (21), a piston (24) is fixed to the bottom end of the lifting rod (23), the outer surface of the piston (24) is slidably connected to the inner surface of the cylinder (21), and an electrode head (25) is fixedly mounted on the inner wall of the cylinder (21).

4. A large amount of microbial culture medium according to claim 1, characterized in that: The incubator (1) is provided with a swing covering assembly (5) for driving the culture fluid falling into the incubator (1) to be evenly covered, the swing covering assembly (5) comprising a cross bar (51) rotatably connected between the inner walls of the incubator (1), a second motor (52) is fixed to one side of the incubator (1), and the second motor (52) drives the cross bar (51) to rotate.

5. A large amount of microbial culture medium according to claim 4, characterized in that: A rotating wheel (53) is fixed on the cross bar (51), and a curved groove (54) is provided on the outer surface of the rotating wheel (53). A connecting plate (55) is fixed on one side of the inner wall of the incubator (1), and a rotating shaft (56) is rotatably connected to the top of the connecting plate (55). A swing rod (57) is fixed to the top of the rotating shaft (56), and a round ball (58) is fixed to one end of the swing rod (57), and the round ball (58) slides inside the curved groove (54). A triangular seat (59) is fixed to the other end of the swing rod (57).

6. A large amount of microbial culture medium according to claim 5, characterized in that: A diverter seat (510) is fixed on both sides of the bottom of the triangular seat (59), and a transverse groove (511) is provided inside the two diverter seats (510). A plurality of inclined grooves (512) are also provided on the inclined surface of the diverter seat (510), and the inclined grooves (512) are connected to the transverse grooves (511).

7. A large amount of microbial culture medium according to claim 1, characterized in that: A culture box (6) is slidably connected to one side of the culture box (1), a microorganism tube (7) is connected to one side of the culture box (1), a flow guide seat (8) is fixed to one side of the inner wall of the culture box (1), a plurality of flow guide grooves (9) are provided on the flow guide seat (8), and a connecting bucket (10) is fixed between the inner walls of the culture box (1).

8. A method for detecting a large amount of microbial culture medium, characterized in that: The specific steps include: Step 1: injecting a base liquid containing microorganisms into the culture box (1) through the microorganism tube (7), the base liquid flows into the liquid receiving bucket (34), and slides along the inclined surface of the liquid receiving bucket (34) to the guide seat (8), and is diverted through the guide groove (9) to allow the base liquid to flow evenly into the culture box (6); Step 2: Pour the culture solution into the liquid bucket (34), start the motor 1 (43), and make the motor 1 (43) drive the rotating rod (42) and the cam (44) to rotate, thereby driving the cam (44) to reciprocate and squeeze the pressing cylinder (36). The convex column (311) on the pressing cylinder (36) slides in the wave groove (310), thereby driving the rotating cylinder (37) to rotate 90 degrees, and at the same time squeezes the compression spring (312). When the pressing cylinder (36) moves downward, it drives The folding rod (313), the vertical rod (314) and the blocking cone (315) move downward at the same time. At this time, after the blocking cone (315) moves downward, part of the culture fluid flows into the space between the two rotating plates (39). As the spring (312) is reset, the blocking cone (315) is reset at the same time and seals the liquid outlet of the pressing cylinder (36), thereby completing the quantitative feeding operation on one side. As the rotating plate (39) rotates, the culture fluid is brought to the fan-shaped groove (33) and falls into the incubator (1); Step 3: After the culture liquid falls onto the triangular seat (59), it slides down along its inclined surface to both sides and falls into the diverter seat (510). After being filled in the transverse groove (511), it is discharged through each inclined groove, so that the culture liquid is evenly diverted. The second motor (52) is started, and the second motor (52) drives the rotating wheel (53) to rotate, thereby driving the ball (58) to slide in the curved groove (54), thereby driving the swing rod (57), the triangular seat (59), and the diverter seat (510) to swing left and right around the rotating shaft (56). During the swinging process, the culture liquid can be evenly sprinkled at various places near and far of the culture box (6), so that the culture liquid evenly covers the microorganisms; Step 4: After waiting for 2 hours for microorganisms to be cultured, the lifting rod (23) is pulled to make the lifting rod (23) drive the piston (24) to move upward, and then the piston (24) extracts a part of the air containing microorganisms in the culture box (1) into the cylinder (21), and then the air containing microorganisms contacts the electrode head (25). As the number of microbial cells increases, the charge on the cell membrane and the difference in ion concentration inside and outside the cell will also increase the contribution to the conductivity of the solution. At the same time, the number of ions generated by microbial metabolism also increases, thereby improving the conductivity. The conductivity is detected using the circuit of the electrode head (25). When the conductivity is high, it means that the number of microorganisms is large. Step 5: After detecting an increase in the number of microorganisms, start the motor 1 (43) again to implement a quantitative feeding operation of the culture solution to increase the concentration of the culture solution and ensure the normal culture of the microorganisms. At the same time, record the number of quantitative feedings, calculate the concentration change of the culture solution based on the number of feedings, and finally draw out the culture box (6) to count the number of microorganisms.

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