Micro-fluidic chip based on DNA strand displacement neuron, method and electronic equipment
By designing a microfluidic chip based on DNA strand replacement neurons, the problem of large size and large footprint of traditional laboratory equipment is solved, precise control and automation of DNA strand replacement operations is achieved, the accuracy of detection or recognition of DNA molecules is improved, and the equipment can be carried with you.
Patent Information
- Application Number
- CN202411995514.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-31
- Publication Date
- 2025-06-06
AI Technical Summary
Traditional laboratory equipment is large in size and covers a large area, so it should not be carried into biological bodies, resulting in inaccurate detection or identification of DNA molecules.
Design a microfluidic chip based on DNA strand replacement neurons, integrates data acquisition module, microfluidic module and DNA strand replacement module, and realizes miniaturization and automation of DNA strand replacement operations through microfluidic technology.
It realizes precise control and automation of DNA strand replacement operations, improves the accuracy of detection or recognition of DNA molecules, and allows the equipment to carry it around and detects DNA molecular signals in a timely manner.
Smart Images
Figure CN120108506A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the field of biological computing technology, and in particular to a microfluidic chip, method and electronic equipment based on DNA chain replacement neurons. Background Art
[0002] Deoxyribonucleic Acid (DNA) computing technology has the ability to adapt to biological, chemical and environmental scenarios, and can realize biological evolution functions such as genetic drift and recombination. It provides a possible solution to the defects of intelligent models based on traditional electronic technology in biology, chemistry and scenario adaptation.
[0003] However, traditional laboratory equipment has problems such as being bulky, occupying a large area, being difficult to carry, and not suitable for embedding in living organisms, which means that research on DNA molecules can only be limited to the laboratory, making it impossible to study DNA molecule signals in a timely manner, resulting in inaccurate detection of DNA molecules. Summary of the invention
[0004] The present invention provides a microfluidic chip, method and electronic device based on DNA chain replacement neurons, which are used to solve the problems of traditional laboratory equipment in the prior art, such as bloated volume, large floor space, non-portability, and inappropriate embedding in biological bodies, which lead to inaccurate detection or identification of DNA molecules. The present invention realizes miniaturization and automation of the replacement operation in an integrated chip, timely detection of DNA molecules, and improved accuracy of detection or identification of DNA molecules.
[0005] The present invention provides a microfluidic chip based on DNA strand displacement neurons, comprising: a data acquisition module 110, a microfluidic module 120 and at least one DNA strand displacement module 130, wherein the microfluidic module 120 is respectively connected to the data acquisition module 110 and the DNA strand displacement module 130, and the data acquisition module 110 is connected to the DNA strand displacement module 130; The data acquisition module 110 is used to acquire a DNA single strand to be replaced and at least one replacement neuron; the replacement neuron is used to perform a replacement on the DNA single strand to be replaced; The microfluidic module 120 is used to drive the DNA single strand to be replaced and the replacement neuron from the data acquisition module 110 to the DNA strand replacement module 130; The DNA strand replacement module 130 is used to determine a target DNA strand after replacement of the DNA strand to be replaced based on the DNA strand to be replaced and each replacement neuron; the target DNA strand is used to identify the DNA strand to be replaced.
[0006] A microfluidic chip based on DNA chain replacement neurons provided by the present invention also includes a reporting module 140, which is respectively connected to the microfluidic module 120 and the DNA chain replacement module 130; the microfluidic module 120 is also used to drive the target DNA single chain from the DNA chain replacement module 130 to the reporting module 140; the reporting module 140 is used to convert the chemical signal of the target DNA single chain into an optical signal; the optical signal is used to detect the target DNA single chain.
[0007] According to a microfluidic chip based on DNA strand displacement neurons provided by the present invention, the reporting module 140 is also used to perform fluorescence detection on the chemical signal of the target DNA single strand to obtain an optical signal corresponding to the target DNA single strand.
[0008] According to a microfluidic chip based on DNA chain replacement neurons provided by the present invention, the DNA chain replacement module 130 includes a DNA origami unit, which is connected to the data acquisition module 110; the DNA origami unit is used to provide a connection strategy for each of the replacement neurons; the DNA origami unit is also used to construct a replacement neural network structure based on the connection strategy and each of the replacement neurons; the DNA origami unit is also used to determine the target DNA single chain based on the DNA single chain to be replaced and the replacement neural network structure when the DNA single chain to be replaced is driven to the replacement neural network structure.
[0009] According to a microfluidic chip based on DNA chain replacement neurons provided by the present invention, the DNA origami unit is further used to pair the fulcrum in the DNA single strand to be replaced with the fulcrum in the first DNA single strand in the DNA double strand to be reacted based on the base pairing principle to obtain a complex containing the DNA single strand to be replaced and the DNA double strand to be reacted; the DNA double strand to be reacted is the DNA double strand in the first replacement neuron in the replacement neural network structure; the DNA origami unit is further used to extend the matching of the DNA single strand to be replaced with the first DNA single strand based on the fulcrum in the complex and the first DNA single strand until the second DNA single strand in the DNA double strand to be reacted is completely separated from the DNA double strand; the second DNA single strand is input into the second replacement neuron in the replacement neural network structure to continue replacement until the last replacement neuron in the replacement neural network structure outputs a replacement result, and the replacement result is determined as the target DNA single strand.
[0010] According to a microfluidic chip based on DNA chain replacement neurons provided by the present invention, the DNA chain replacement module 130 also includes: an environment adjustment unit, which is connected to the DNA origami unit; the environment adjustment unit is used to adjust the reaction environment information of the DNA single chain to be replaced and each of the replacement neurons when the replacement efficiency corresponding to the target DNA single chain does not meet the preset efficiency, so as to change the connection strategy of each of the replacement neurons and obtain an adjusted replacement neural network structure; the adjusted replacement neural network structure is used to adjust the replacement efficiency corresponding to the target DNA single chain.
[0011] A microfluidic chip based on DNA chain replacement neurons provided by the present invention also includes: a fulcrum adjustment unit, which is respectively connected to the data acquisition module 110 and the DNA chain replacement module 130; the fulcrum adjustment unit is used to adjust the concentration of the DNA single chain to be replaced when the replacement efficiency corresponding to the target DNA single chain does not meet the preset efficiency, so as to obtain the adjusted DNA single chain to be replaced, and the adjusted DNA single chain to be replaced is used to adjust the replacement efficiency corresponding to the target DNA single chain.
[0012] According to a microfluidic chip based on DNA chain replacement neurons provided by the present invention, it also includes a DNA single-chain storage module and a replacement neuron storage module, and the data acquisition module 110 is connected to the DNA single-chain storage module and the replacement neuron storage module respectively; the DNA single-chain storage module is used to provide the DNA single chain to be replaced to the data acquisition module 110; the replacement neuron storage module is used to provide the replacement neuron to the data acquisition module 110.
[0013] The present invention also provides a microfluidic method based on DNA strand displacement neurons, which is applied to the above-mentioned microfluidic chip based on DNA strand displacement neurons, comprising: Acquire the DNA single strand to be replaced and at least one replacement neuron through the data acquisition module 110; the replacement neuron is used to perform a replacement on the DNA single strand to be replaced; The microfluidic module 120 drives the DNA single strand to be replaced and the replacement neuron from the data acquisition module 110 to the DNA strand replacement module 130; The DNA strand replacement module 130 determines the target DNA strand after the replacement of the DNA strand to be replaced based on the DNA strand to be replaced and each replacement neuron; the target DNA strand is used to identify the DNA strand to be replaced.
[0014] The present invention also provides an electronic device, comprising a memory, a processor, and a computer program stored in the memory and executable on the processor, wherein when the processor executes the computer program, the microfluidic method based on DNA chain replacement neurons as described above is implemented.
[0015] The present invention also provides a non-transitory computer-readable storage medium having a computer program stored thereon, which, when executed by a processor, implements any of the above-described microfluidic methods based on DNA chain replacement neurons.
[0016] The present invention also provides a computer program product, comprising a computer program, wherein when the computer program is executed by a processor, the microfluidic method based on DNA chain replacement neurons as described above is implemented.
[0017] The microfluidic chip, method and electronic device based on DNA chain replacement neurons provided by the present invention are achieved by concentrating a data acquisition module 110, a microfluidic module 120 and at least one DNA chain replacement module 130 on one chip, and then using the data acquisition module 110 to acquire the DNA single chain to be replaced and at least one replacement neuron; the microfluidic module 120 drives the DNA single chain to be replaced and the replacement neuron from the data acquisition module 110 to the DNA chain replacement module 130, thereby improving the precise control of DNA; the DNA chain replacement module 130 determines the target DNA single chain after the replacement of the DNA single chain to be replaced based on the DNA single chain to be replaced and each replacement neuron, so that the miniaturization and automation of the DNA chain replacement operation can be realized by using the integrated chip, and the microfluidic chip based on the DNA chain replacement neuron can be carried around to facilitate the timely detection of DNA molecule signals, thereby improving the detection accuracy of DNA molecules. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] In order to more clearly illustrate the technical solutions in the present invention or the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.
[0019] Figure 1 This is one of the structural schematic diagrams of the microfluidic chip based on DNA strand displacement neurons provided by the present invention.
[0020] Figure 2 This is the second structural schematic diagram of the microfluidic chip based on DNA strand displacement neurons provided by the present invention.
[0021] Figure 3 It is a schematic structural diagram of the reaction between the single-stranded DNA to be replaced and the replacement neuron provided by the present invention.
[0022] Figure 4 It is a structural schematic diagram of the cascade reaction between the single-stranded DNA to be replaced and the replacement neural network structure provided by the present invention.
[0023] Figure 5 It is a schematic diagram of the connection structure of DNA molecules corresponding to different illuminations provided by the present invention.
[0024] Figure 6 This is the third structural schematic diagram of the microfluidic chip based on DNA strand displacement neurons provided by the present invention.
[0025] Figure 7 It is a schematic flow chart of the microfluidic method based on DNA strand displacement neurons provided by the present invention.
[0026] Figure 8 It is a structural schematic diagram of the electronic device provided by the present invention.
[0027] Reference numerals: 100: microfluidic chip based on DNA strand displacement neurons; 110: data acquisition module; 120: microfluidic module; 130: DNA strand displacement module; 140: reporting module. DETAILED DESCRIPTION
[0028] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be clearly and completely described below in conjunction with the drawings of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.
[0029] At present, some studies have realized the functions of small-scale neural networks and simulated neurons through DNA chain displacement technology, but these studies still have shortcomings in flexibility, scalability and environmental adaptability. In addition, traditional laboratory equipment has problems such as bloated size, large footprint, non-portability, and unsuitable for embedding in organisms, which makes the research on DNA molecules limited to the laboratory, making it impossible to study DNA molecules in a timely manner, resulting in inaccurate detection or identification of DNA molecules.
[0030] Combine the following Figure 1-Figure 6 The present invention describes a microfluidic chip based on DNA strand displacement neurons, which can realize the study of any biological DNA molecules, including but not limited to humans, animals or plants.
[0031] Figure 1 is one of the structural schematic diagrams of the microfluidic chip based on DNA strand displacement neurons provided by the present invention, such as Figure 1 As shown, the microfluidic chip 100 based on DNA strand displacement neurons includes: a data acquisition module 110, a microfluidic module 120 and at least one DNA strand displacement module 130; the microfluidic module 120 is respectively connected to the data acquisition module 110 and the DNA strand displacement module 130, and the data acquisition module 110 is connected to the DNA strand displacement module 130; The data acquisition module 110 is used to acquire a DNA single strand to be replaced and at least one replacement neuron; the replacement neuron is used to perform a replacement on the DNA single strand to be replaced; The microfluidic module 120 is used to drive the DNA single strand to be replaced and the replacement neuron from the data acquisition module 110 to the DNA strand replacement module 130; The DNA strand replacement module 130 is used to determine a target DNA strand after replacement of the DNA strand to be replaced based on the DNA strand to be replaced and each replacement neuron; the target DNA strand is used to identify the DNA strand to be replaced.
[0032] Here, the DNA single strand to be replaced can be a DNA single strand of any organism, and the replacement neuron contains a double strand of DNA. Among them, the DNA single strand to be replaced is the input of the replacement neuron. The DNA single strand to be replaced can be obtained by single-stranded DNA encoding.
[0033] It should be noted that the DNA single strand to be replaced reacts with the replacement neuron through the exposed pivot point to obtain the replaced DNA single strand. Here, if the replaced DNA single strand has an exposed pivot point, a strand replacement reaction in the opposite direction can occur again.
[0034] It should be noted that the DNA molecules in the present invention are all in the form of droplets. The droplets of DNA molecules to be replaced are input into the data acquisition module 110, and the microfluidic module 120 drives the droplets of DNA molecules to be replaced to the DNA chain replacement module 130 through microfluidic technology. Here, the microcontrol module can also perform quantitative droplet drive on DNA. After reasonable planning of the liquid path, the single-stranded DNA to be replaced and the replacement neurons are driven to the DNA chain replacement module 130 according to the amount, so as to realize precise control of the input quantity.
[0035] Here, the implementation of the microfluidic module 120 includes but is not limited to motor drive, light drive, etc.
[0036] Here, the DNA strand replacement module 130 is also a reaction module. After driving the DNA strand to be replaced and the replacement neuron to the reaction module, the DNA strand to be replaced and the replacement neuron undergo a replacement reaction to obtain a target DNA strand. The target DNA strand may be obtained after passing through one replacement neuron or after passing through multiple replacement neurons.
[0037] In an embodiment of the invention, the data acquisition module 110, the microfluidic module 120 and at least one DNA chain replacement module 130 are concentrated on one chip, and then the data acquisition module 110 is used to acquire the DNA single chain to be replaced and at least one replacement neuron; the microfluidic module 120 drives the DNA single chain to be replaced and the replacement neuron from the data acquisition module 110 to the DNA chain replacement module 130, thereby improving the precise control of DNA; the DNA chain replacement module 130 determines the target DNA single chain after the replacement of the DNA single chain to be replaced based on the DNA single chain to be replaced and each replacement neuron, so that the miniaturization and automation of the DNA chain replacement operation can be realized by using the integrated chip, and the microfluidic chip based on the DNA chain replacement neuron can be carried around to facilitate the timely detection of DNA molecule signals, thereby improving the detection accuracy of DNA molecules.
[0038] further, Figure 2 FIG. 2 is a schematic diagram of the structure of a microfluidic chip based on DNA strand displacement neurons provided by the present invention. Figure 2 As shown, the microfluidic chip 100 based on DNA strand displacement neurons further includes a reporting module 140, and the reporting module 140 is respectively connected to the microfluidic module 120 and the DNA strand displacement module 130; The microfluidic module 120 is also used to drive the target DNA single strand from the DNA strand displacement module 130 to the reporting module 140; The reporting module 140 is used to convert the chemical signal of the target DNA single strand into an optical signal; the optical signal is used to detect the target DNA single strand.
[0039] Here, the microfluidic module 120 drives the target DNA single strand obtained after the reaction from the DNA strand displacement module 130 to the reporting module 140 , and displays the optical signal of the target DNA single strand in the reporting module 140 .
[0040] Here, the reporting module 140 may convert the target DNA single strand from a chemical signal to an optical signal by performing fluorescence detection on the chemical signal of the target DNA single strand to obtain an optical signal corresponding to the target DNA single strand.
[0041] It should be noted that optical signals are easier to detect than chemical signals. The optical signal of the target DNA single strand is essentially a fluorescent group bound to the DNA chain. Different DNA components are labeled with different fluorescent markers to identify the genetic components of the target DNA single strand.
[0042] In another embodiment, the target DNA single strand can also be detected and identified from a quantitative perspective based on the fluorescence intensity.
[0043] In the embodiment of the present invention, the target DNA single strand is fluorescently detected by the reporter module 140, and the chemical signal is converted into an optical signal that is easy to detect, thereby realizing the observation and regulation of the target DNA single strand.
[0044] Further, the DNA strand displacement module 130 includes a DNA origami unit, and the origami unit is connected to the data acquisition module 110; The DNA origami unit is used to provide a connection strategy for each of the replacement neurons; The DNA origami unit is also used to construct a replacement neural network structure based on the connection strategy and each replacement neuron; The DNA origami unit is also used to determine the target DNA single strand based on the DNA single strand to be replaced and the replacement neural network structure when the DNA single strand to be replaced is driven to the replacement neural network structure.
[0045] Here, the connection strategy includes the connection structure and arrangement order between each replacement neuron. It should be noted that the connection strategy can be designed in advance, and the replacement neuron can be arranged according to the connection structure and arrangement order in the connection strategy to obtain a replacement neural network structure.
[0046] It should be noted that since a single replacement neuron is in a diffuse state in the solution, multiple replacement neurons cannot react in the set order. Therefore, a DNA origami unit is needed to integrate the replacement neurons together in order, so as to design a neural network structure with a certain order and topological structure.
[0047] In an embodiment of the present invention, a variety of large-scale permutation neural network structures such as multi-input single output, singular single output, single input multiple output, etc. can be constructed through DNA origami units to improve the controllability and scalability of the permutation neural network.
[0048] Furthermore, the DNA origami unit is also used to pair the support point in the to-be-replaced DNA single strand with the support point in the first DNA single strand in the to-be-reacted DNA double strand based on the base pairing principle, so as to obtain a complex comprising the to-be-replaced DNA single strand and the to-be-reacted DNA double strand; the to-be-reacted DNA double strand is the DNA double strand in the first replacement neuron in the replacement neural network structure; The DNA origami unit is also used to extend the matching of the to-be-replaced DNA single strand with the first DNA single strand based on the fulcrum in the complex and the first DNA single strand, until the second DNA single strand in the to-be-reacted DNA double strand is completely separated from the DNA double strand; the second DNA single strand is input into the second replacement neuron in the replacement neural network structure to continue replacement, until the last replacement neuron in the replacement neural network structure outputs a replacement result, and the replacement result is determined as the target DNA single strand.
[0049] It should be noted that a fulcrum refers to a sequence that can complementarily pair with a sequence in the DNA double helix, and its length is usually between 3 and 5 bases.
[0050] It should be noted that there are exposed pivots in the single-stranded DNA to be replaced, which can react with the replacement neurons. In this process, the single-stranded DNA to be replaced forms a hydrogen bond with the double-stranded DNA, resulting in the separation of the two strands of the double-stranded DNA, and obtaining a single-stranded DNA target, which is the output strand.
[0051] It should be noted that the reaction between the DNA single strand to be replaced and the replacement neural network structure is essentially that the DNA single strand to be replaced reacts with the first replacement neuron in the replacement neural network to obtain the first output; the first output reacts with the second replacement neuron to obtain the second output; the second output reacts with the third replacement neuron, until the output of the replacement neuron at the previous level reacts with the last replacement neuron to obtain the target DNA single strand. In other words, the replacement neural network structure is a cascade structure.
[0052] For example, Figure 3 Schematic diagram of the reaction between the single-stranded DNA to be replaced and the replacement neuron provided by the present invention, such as Figure 3 As shown, (2,3) is the input chain, {(1,2)(2*,3*)} is a single replacement neuron, 3 is the "fulcrum" that matches 3* to form a complex containing {(2,3)(1,2)(2*,3*)}, the input chain continues to react with the replacement neuron, the input chain continues to extend forward, causing the end of the (1,2) chain to detach, forming the output (1,2) DNA chain, the input chain reacts with the replacement neuron to generate the output chain (1,2) and the waste {(2,3)(2*,3*)}. The waste chain exists in the form of a double-stranded DNA chain, and the "fulcrum" no longer exists.
[0053] For example, Figure 4 Schematic diagram of the cascade reaction between the single-stranded DNA to be replaced and the replacement neural network structure provided by the present invention, such as Figure 4As shown, the cascade reaction between the DNA single strand to be replaced and the replacement neural network structure can be called an integration function. The integration function can adjust the signal. If the input is a negative signal (X-), that is, the above-mentioned DNA single strand to be replaced (there are negative signals XL-, XR- at both ends), the reaction system can eventually generate SP4 representing a negative signal, XR- (only one section of negative signal XL-). If a positive signal X+ (XL+, XR+) is added, a positive signal SP6 (only one section of positive signal XR+) can be generated. In this way, the positive and negative inputs can be adjusted through this system (three-step chain replacement). In the figure, L represents left and R represents right.
[0054] In the embodiment of the present invention, the strand displacement reaction in the displacement neuron network has a cascade property, and the output of the previous reaction can be used as the input of the next reaction, thereby expanding the scale of the DNA strand displacement reaction and realizing complex functions.
[0055] Further, the DNA strand displacement module 130 further includes: an environment adjustment unit, the environment adjustment unit being connected to the DNA origami unit; The environment adjustment unit is used to adjust the reaction environment information of the DNA single strand to be replaced and each of the replacement neurons when the replacement efficiency corresponding to the target DNA single strand does not meet the preset efficiency, so as to change the connection strategy of each of the replacement neurons and obtain an adjusted replacement neural network structure; the adjusted replacement neural network structure is used to adjust the replacement efficiency corresponding to the target DNA single strand.
[0056] Here, the preset efficiency can be set by the staff based on experience or obtained based on multiple experiments.
[0057] Here, environmental information includes but is not limited to light, temperature, etc.
[0058] Figure 5 is a schematic diagram of the connection structure of DNA molecules corresponding to different illuminations provided by the present invention, such as Figure 5 As shown, under ultraviolet light (UV Light), the connection structure of DNA molecules changes from left to right, and under blue light (Blue Light), the connection structure of DNA molecules changes from right to left. It can be seen that the connection structure of DNA molecules is different under different light.
[0059] It should be noted that in the replacement neural network structure, the higher the reaction efficiency, the better the effect of the replacement neural network structure. Therefore, after each operation of the replacement neural network structure, the reaction efficiency should be calculated. When the reaction efficiency does not meet the preset efficiency, the reaction environment should be adjusted in time to replace the connection structure of the neurons and obtain the adjusted replacement neural network structure. The single-stranded DNA to be replaced is further reacted with the adjusted replacement neural network structure to detect the reaction efficiency of the adjusted replacement neural network structure. When the reaction efficiency still does not meet the preset efficiency, continue to adjust the ambient temperature and change the connection structure of the replacement neurons until the reaction efficiency of the adjusted replacement neural network structure meets the preset efficiency to obtain the target replacement neural network structure.
[0060] In the embodiment of the present invention, the replacement neural network structure is adjusted by changing the reaction environment information to improve the replacement reaction efficiency, and the rate and stability of the DNA chain replacement reaction can also be improved.
[0061] Further, it also includes: a fulcrum adjustment unit, the fulcrum adjustment unit is respectively connected to the data acquisition module 110 and the DNA strand displacement module 130; The fulcrum adjustment unit is used to adjust the concentration of the DNA single strand to be replaced when the replacement efficiency corresponding to the target DNA single strand does not meet the preset efficiency, so as to obtain the adjusted DNA single strand to be replaced, and the adjusted DNA single strand to be replaced is used to adjust the replacement efficiency corresponding to the target DNA single strand.
[0062] It should be noted that the efficiency of the DNA strand displacement reaction is not only affected by the environment, but also by the concentration of the DNA single strand to be replaced.
[0063] It should be noted that the length of the fulcrum corresponds to the concentration of the single-stranded DNA to be replaced. The longer the fulcrum, the higher the concentration and the higher the reaction efficiency; the shorter the fulcrum, the lower the concentration and the lower the reaction efficiency.
[0064] In the embodiment of the present invention, the concentration of the DNA single strand to be replaced, that is, the length and sequence of the fulcrum, is controlled to control the reaction efficiency of the replacement neuron, and further, the reaction speed and intensity of the replacement neural network structure to different signals are controlled.
[0065] Furthermore, it also includes a DNA single-strand storage module and a replacement neuron storage module, and the data acquisition module 110 is connected to the DNA single-strand storage module and the replacement neuron storage module respectively; The DNA single-strand storage module is used to provide the DNA single-strand to be replaced to the data acquisition module 110; The replacement neuron storage module is used to provide the replacement neuron to the data acquisition module 110.
[0066] In the embodiment of the present invention, by providing the data acquisition module 110 with the DNA single strand to be replaced and the replacement neuron, a data basis is provided for the microfluidic chip.
[0067] For example, Figure 6 FIG. 3 is a schematic diagram of the structure of a microfluidic chip based on DNA strand displacement neurons provided by the present invention. Figure 6 As shown, the microfluidic chip based on DNA strand displacement neurons includes a DNA single-strand storage module, namely Figure 6 Middle 、b 、c-in module, used to provide a DNA single strand to be replaced; the replacement neuron storage module is Figure 6 The switch in is used to provide replacement neurons; the DNA origami unit is Figure 6 Blocks 1, 2, 3, and 4 are used to determine the target DNA strand based on the DNA strand to be replaced and the replacement neural network structure when the DNA strand to be replaced is driven to the replacement neural network structure; the reporting module 140 is Figure 6 Reporter-1 and Reporter-2 in the above diagram are used to convert the chemical signal of the target DNA single strand into an optical signal that is easy to detect. After the replacement operation of the DNA single strand to be replaced is completed, the waste liquid generated will be stored in the waste liquid unit. Figure 6 Waste liquid in.
[0068] First, the motor drive in the microfluidic chip based on DNA chain replacement neurons (i.e., the above-mentioned microfluidic module 120) drives a certain number of replacement neurons to a specific DNA origami unit position. DNA origami can be understood as a blank PCB board (the PCB board is fixed at this position). When the replacement neurons are driven to the corresponding DNA origami unit positions (1, 2, 3, 4), the replacement neurons are connected to the base of the DNA origami unit to form a replacement neural network structure with a set topological structure; secondly, the motor drive (i.e., the above-mentioned microfluidic module 120) inputs a certain number of DNA single chains to be replaced into the corresponding replacement neural network structure (1, 2, 3 or 4 positions), allowing the DNA single chains to be replaced to be replaced and the replacement neural network structure (on the fixed neural network), and obtain the final target DNA single chain (free state), which is bound to a fluorescent group; finally, the motor drive (i.e., the above-mentioned microfluidic module 120) drives the free target DNA single chain to the reporting module 140 ( Figure 6 In the reporter 1 or reporter 2, there is a fluorescence detection probe at the reporter to detect the output fluorescence signal. The fluorescent groups have different colors, which can distinguish the results. In addition, the fluorescence intensity detects the results from a quantitative perspective.
[0069] In an embodiment of the present invention, a microfluidic chip based on DNA chain replacement neurons controls the movement of droplets representing each neural network unit to a specific area for mixed calculations, and detects optical signals after the calculation to obtain experimental results.
[0070] The microfluidic method based on DNA strand displacement neurons provided by the present invention is described below. The microfluidic method based on DNA strand displacement neurons described below and the microfluidic chip based on DNA strand displacement neurons described above can correspond to each other.
[0071] Figure 7 is a schematic diagram of the process of the microfluidic method based on DNA strand displacement neurons provided by the present invention, such as Figure 7 As shown, the method is applied to the above-mentioned microfluidic chip based on DNA strand displacement neurons, and the method comprises: Step 701 : Acquire a single-stranded DNA to be replaced and at least one replacement neuron through the data acquisition module 110 .
[0072] The replacement neuron is used to perform a replacement on the single-stranded DNA to be replaced.
[0073] Step 702 : Drive the to-be-replaced DNA single strand and the replacement neuron from the data acquisition module 110 to the DNA strand replacement module 130 through the microfluidic module 120 .
[0074] Step 703: Determine, by means of the DNA strand replacement module 130, a target DNA strand after replacement of the DNA strand to be replaced based on the DNA strand to be replaced and each replacement neuron.
[0075] The target DNA single strand is used to identify the DNA single strand to be replaced.
[0076] Figure 8 is a schematic diagram of the structure of the electronic device provided by the present invention, such as Figure 8As shown, the electronic device may include: a processor 810, a communication interface 820, a memory 830 and a communication bus 840, wherein the processor 810, the communication interface 820 and the memory 830 communicate with each other through the communication bus 840. The processor 810 may call the logic instructions in the memory 830 to execute a microfluidic method based on DNA chain replacement neurons, the method comprising: obtaining a DNA single strand to be replaced and at least one replacement neuron through a data acquisition module 110; the replacement neuron is used to perform a replacement on the DNA single strand to be replaced; driving the DNA single strand to be replaced and the replacement neuron from the data acquisition module 110 to the DNA chain replacement module 130 through a microfluidic module 120; determining a target DNA single strand after the replacement of the DNA single strand to be replaced based on the DNA single strand to be replaced and each replacement neuron through the DNA chain replacement module 130; the target DNA single strand is used to identify the DNA single strand to be replaced.
[0077] In addition, the logic instructions in the above-mentioned memory 830 can be implemented in the form of a software functional unit and can be stored in a computer-readable storage medium when it is sold or used as an independent product. Based on this understanding, the technical solution of the present invention is essentially or the part that contributes to the prior art or the part of the technical solution can be embodied in the form of a software product, and the computer software product is stored in a storage medium, including a number of instructions to enable a computer device (which can be a personal computer, a server, or a network device, etc.) to perform all or part of the steps of the method described in each embodiment of the present invention. The aforementioned storage medium includes: U disk, mobile hard disk, read-only memory (ROM, Read-Only Memory), random access memory (RAM, Random Access Memory), disk or optical disk and other media that can store program codes.
[0078] On the other hand, the present invention also provides a computer program product, which includes a computer program, and the computer program can be stored on a non-transitory computer-readable storage medium. When the computer program is executed by a processor, the computer can execute the microfluidic method based on DNA chain replacement neurons provided by the above methods, and the method includes: acquiring a DNA single chain to be replaced and at least one replacement neuron through a data acquisition module 110; the replacement neuron is used to perform a replacement on the DNA single chain to be replaced; driving the DNA single chain to be replaced and the replacement neuron from the data acquisition module 110 to the DNA chain replacement module 130 through a microfluidic module 120; determining a target DNA single chain after replacement of the DNA single chain to be replaced based on the DNA single chain to be replaced and each of the replacement neurons through the DNA chain replacement module 130; the target DNA single chain is used to identify the DNA single chain to be replaced.
[0079] On the other hand, the present invention also provides a non-transitory computer-readable storage medium having a computer program stored thereon, which, when executed by a processor, is implemented to execute the microfluidic method based on DNA chain replacement neurons provided by the above-mentioned methods, the method comprising: acquiring a DNA single chain to be replaced and at least one replacement neuron through a data acquisition module 110; the replacement neuron is used to perform a replacement on the DNA single chain to be replaced; driving the DNA single chain to be replaced and the replacement neuron from the data acquisition module 110 to the DNA chain replacement module 130 through a microfluidic module 120; determining a target DNA single chain after replacement of the DNA single chain to be replaced based on the DNA single chain to be replaced and each of the replacement neurons through the DNA chain replacement module 130; the target DNA single chain is used to identify the DNA single chain to be replaced.
[0080] The device embodiments described above are merely illustrative, wherein the units described as separate components may or may not be physically separated, and the components displayed as units may or may not be physical units, that is, they may be located in one place, or they may be distributed on multiple network units. Some or all of the modules may be selected according to actual needs to achieve the purpose of the scheme of this embodiment. Ordinary technicians in this field can understand and implement it without paying creative labor.
[0081] Through the description of the above implementation methods, those skilled in the art can clearly understand that each implementation method can be implemented by means of software plus a necessary general hardware platform, and of course, can also be implemented by hardware. Based on this understanding, the above technical solution is essentially or the part that contributes to the prior art can be embodied in the form of a software product, and the computer software product can be stored in a computer-readable storage medium, such as ROM / RAM, a disk, an optical disk, etc., including a number of instructions for a computer device (which can be a personal computer, a server, or a network device, etc.) to execute the methods described in each embodiment or some parts of the embodiments.
[0082] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.
Claims
1. A microfluidic chip based on DNA strand replacement neurons, characterized in that: include: A data acquisition module 110, a microfluidic module 120 and at least one DNA strand displacement module 130, wherein the microfluidic module 120 is connected to the data acquisition module 110 and the DNA strand displacement module 130 respectively; the data acquisition module 110 is connected to the DNA strand displacement module 130; The data acquisition module 110 is used to acquire a DNA single strand to be replaced and at least one replacement neuron; the replacement neuron is used to perform a replacement on the DNA single strand to be replaced; The microfluidic module 120 is used to drive the DNA single strand to be replaced and the replacement neuron from the data acquisition module 110 to the DNA strand replacement module 130; The DNA strand replacement module 130 is used to determine a target DNA strand after replacement of the DNA strand to be replaced based on the DNA strand to be replaced and each replacement neuron; the target DNA strand is used to identify the DNA strand to be replaced.
2. The microfluidic chip based on DNA strand displacement neurons according to claim 1, characterized in that: It also includes a reporting module 140, and the reporting module 140 is connected to the microfluidic module 120 and the DNA strand displacement module 130 respectively; The microfluidic module 120 is also used to drive the target DNA single strand from the DNA strand displacement module 130 to the reporting module 140; The reporting module 140 is used to convert the chemical signal of the target DNA single strand into an optical signal; the optical signal is used to detect the target DNA single strand.
3. The microfluidic chip based on DNA strand displacement neurons according to claim 2, characterized in that: The reporting module 140 is also used to perform fluorescence detection on the chemical signal of the target DNA single strand to obtain an optical signal corresponding to the target DNA single strand.
4. The microfluidic chip based on DNA strand displacement neurons according to any one of claims 1 to 3, characterized in that: The DNA strand displacement module 130 includes a DNA origami unit, and the origami unit is connected to the data acquisition module 110; The DNA origami unit is used to provide a connection strategy for each of the replacement neurons; The DNA origami unit is also used to construct a replacement neural network structure based on the connection strategy and each replacement neuron; The DNA origami unit is also used to determine the target DNA single strand based on the DNA single strand to be replaced and the replacement neural network structure when the DNA single strand to be replaced is driven to the replacement neural network structure.
5. The microfluidic chip based on DNA strand displacement neurons according to claim 4, characterized in that: The DNA origami unit is further used to pair the support point in the to-be-replaced DNA single strand with the support point in the first DNA single strand in the to-be-reacted DNA double strand based on the base pairing principle, so as to obtain a complex comprising the to-be-replaced DNA single strand and the to-be-reacted DNA double strand; the to-be-reacted DNA double strand is the DNA double strand in the first replacement neuron in the replacement neural network structure; The DNA origami unit is also used to extend the matching of the to-be-replaced DNA single strand with the first DNA single strand based on the fulcrum in the complex and the first DNA single strand, until the second DNA single strand in the to-be-reacted DNA double strand is completely separated from the DNA double strand; the second DNA single strand is input into the second replacement neuron in the replacement neural network structure to continue replacement, until the last replacement neuron in the replacement neural network structure outputs a replacement result, and the replacement result is determined as the target DNA single strand.
6. The microfluidic chip based on DNA strand displacement neurons according to claim 4, characterized in that: The DNA strand displacement module 130 further includes: an environment adjustment unit, the environment adjustment unit being connected to the DNA origami unit; The environment adjustment unit is used to adjust the reaction environment information of the DNA single strand to be replaced and each of the replacement neurons when the replacement efficiency corresponding to the target DNA single strand does not meet the preset efficiency, so as to change the connection strategy of each of the replacement neurons and obtain an adjusted replacement neural network structure; the adjusted replacement neural network structure is used to adjust the replacement efficiency corresponding to the target DNA single strand.
7. The microfluidic chip based on DNA strand displacement neurons according to any one of claims 1 to 3, characterized in that: Also includes: A pivot adjustment unit, the pivot adjustment unit is connected to the data acquisition module 110 and the DNA strand displacement module 130 respectively; The fulcrum adjustment unit is used to adjust the concentration of the DNA single strand to be replaced when the replacement efficiency corresponding to the target DNA single strand does not meet the preset efficiency, so as to obtain the adjusted DNA single strand to be replaced, and the adjusted DNA single strand to be replaced is used to adjust the replacement efficiency corresponding to the target DNA single strand.
8. The microfluidic chip based on DNA strand displacement neurons according to any one of claims 1 to 3, characterized in that: It also includes a DNA single-strand storage module and a replacement neuron storage module, and the data acquisition module 110 is connected to the DNA single-strand storage module and the replacement neuron storage module respectively; The DNA single-strand storage module is used to provide the DNA single-strand to be replaced to the data acquisition module 110; The replacement neuron storage module is used to provide the replacement neuron to the data acquisition module 110.
9. A microfluidic method based on DNA strand displacement neurons, applied to the microfluidic chip based on deoxyribonucleic acid DNA strand displacement neurons according to claim 1, characterized in that: include: Acquire the DNA single strand to be replaced and at least one replacement neuron through the data acquisition module 110; the replacement neuron is used to perform a replacement on the DNA single strand to be replaced; The microfluidic module 120 drives the DNA single strand to be replaced and the replacement neuron from the data acquisition module 110 to the DNA strand replacement module 130; The DNA strand replacement module 130 determines the target DNA strand after the replacement of the DNA strand to be replaced based on the DNA strand to be replaced and each replacement neuron; the target DNA strand is used to identify the DNA strand to be replaced.
10. An electronic device comprising a memory, a processor, and a computer program stored in the memory and running on the processor, characterized in that: When the processor executes the computer program, the microfluidic method based on DNA strand replacement neurons as claimed in claim 9 is implemented.