Adhesive patch for oral ulcer based on pig esophageal mucosa acellular extracellular matrix and preparation method of adhesive patch
Through adhesion patches based on the extracellular matrix of the pig esophageal mucosal decellularization, the problem of the short drug retention time of existing oral ulcer treatment preparations in high humidity environments is solved, and the effect of adhering to the ulcer and promoting healing is achieved, which significantly improves the therapeutic effect and biological activity.
Patent Information
- Application Number
- CN202510301704.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-14
- Publication Date
- 2025-06-10
AI Technical Summary
The existing oral ulcer treatment preparations have short drug retention time in high humidity and multi-protein environments and are easily diluted by saliva or eroded by food, resulting in poor treatment effects and lack of sufficient biological activity, resulting in limited therapeutic effects and prone to recurrence.
Adhesive patches based on the extracellular matrix of the esophageal mucosal decellularization of pig esophageal mucosa were used. They were sealed and stirred, dialysis and lyophilized treatment of lyophilized powder and dopamine hydrochloride buffer solution, combined with methacrylate hyaluronic acid and anti-inflammatory drugs, and cured and lyophilized by ultraviolet light and freeze-dried to prepare oral ulcer adhesion patches with good adhesion properties and biological activity.
This adhesion patch can effectively adhere to oral ulcers, promotes oral tissue damage healing and mucosal inflammation regression, significantly improves the therapeutic effect, reduces the area of ulcers, and reduces inflammation of oral mucosal tissue.
Smart Images
Figure CN120114418A_ABST
Abstract
Description
Technical Field
[0001] The present invention belongs to the technical field of biomedical materials, and particularly relates to an adhesive patch for oral ulcers based on porcine esophageal mucosa acellular extracellular matrix and a preparation method thereof. Background Art
[0002] Oral ulcer is a common oral mucosal disease characterized by persistent epithelial defect and integrity destruction, and its incidence rate can reach 20%-30% in the general population. Oral ulcer not only affects the physiological functions of patients such as chewing, swallowing and speech, but repeated attacks may lead to immune function disorder, cause various complications, and even increase the risk of oral canceration. At present, the commonly used local treatment preparations in clinical practice include mouthwashes, creams, gels, powders and patches, etc. Although they can directly act on the ulcer surface and are easy to operate, due to the unique high humidity of the oral cavity, the presence of various proteins and mucins, and frequent physiological activities such as chewing and swallowing, these preparations have a short drug retention time, are easily diluted by saliva or washed away by food, thus seriously reducing the treatment effect. In addition, the existing preparations mainly rely on inhibiting the inflammatory reaction on the mucosal surface and the self-repair ability of the oral mucosa, lacking sufficient biological activity, resulting in limited treatment effect and easy recurrence.
[0003] Acellular extracellular matrix is a natural biomaterial obtained by removing immunogenic components in organs or tissues through decellularization technology. After being treated by the decellularization technology, the acellular extracellular matrix reduces the immunogenic risk to the lowest level, maximally retains the activity of molecules such as collagen and glycosaminoglycan in the natural extracellular matrix, enabling it to provide strong biological activity support for tissue cells under the condition of no exogenous growth factors, and thus is widely used in tissue regeneration and organ repair. However, at present, the strategy of directly using the biological activity of acellular extracellular matrix materials to induce oral mucosal tissue regeneration has not been fully developed.
[0004] In recent years, inspired by the natural wet adhesion phenomenon of mussels, dopamine and its derivatives have achieved effective adhesion with in-vivo tissues through the synergistic physical and chemical actions between their catechol groups and specific functional groups on the tissue surface. The adhesive materials modified by dopamine materials, as effective carriers for wet tissue repair and local drug delivery, exhibit excellent wet environment adaptability and patient compliance. Combining the biological activity of acellular extracellular matrix and the mussel-inspired adhesion characteristics, developing new and efficient oral mucosal repair materials has become a new idea and preparation strategy. Summary of the Invention
[0005] The object of the present invention is to provide an adhesive patch for oral ulcers based on porcine esophageal mucosa acellular extracellular matrix and a preparation method thereof. The adhesive patch based on porcine esophageal mucosa acellular extracellular matrix prepared by the present invention shows, on the one hand, an adhesion force suitable for oral mucosa and can effectively adhere to oral ulcers, and on the other hand, can play a role in promoting proliferation and anti-inflammation, promoting the healing of oral tissue damage and the regression of mucosal inflammation, so as to achieve the effect of effectively treating oral ulcers.
[0006] To achieve the above object, the present invention adopts the following technical solutions: An adhesive patch based on porcine esophageal mucosa acellular extracellular matrix, and its preparation method includes the following steps: 1) Freeze-dried powder of porcine esophageal mucosa acellular extracellular matrix and a buffer solution containing dopamine hydrochloride are sealed and stirred at room temperature, and then dialyzed and freeze-dried to obtain an adhesive based on porcine esophageal mucosa acellular extracellular matrix; 2) The adhesive based on porcine esophageal mucosa acellular extracellular matrix obtained in step 1) and methacrylated hyaluronic acid are dissolved in deionized water together, and an anti-inflammatory drug and a photoinitiator are sequentially added under light-shielded conditions, and stirred evenly to obtain a mixed solution; 3) The mixed solution obtained in step 2) is injected into a polytetrafluoroethylene mold, cured by ultraviolet light irradiation, and then freeze-dried to obtain the adhesive patch for oral ulcers based on porcine esophageal mucosa acellular extracellular matrix.
[0007] Further, the preparation of the freeze-dried powder of porcine esophageal mucosa acellular extracellular matrix in step 1) is to strip the porcine esophageal mucosa tissue from the porcine esophagus, and then after degreasing, decellularization, DNA removal and simulated gastric juice digestion treatment, adjust the pH of the digestion solution to 7, and then obtain it by freeze-drying and grinding.
[0008] Further, the appearance of the porcine esophageal mucosa acellular extracellular matrix is white, and its DNA content is less than 50 ng / mg.
[0009] Further, the buffer solution containing dopamine hydrochloride in step 1) is a MES buffer solution containing 0.5 - 3 mM (preferably 2 mM) dopamine hydrochloride, 0.2 - 1 mM (preferably 0.5 mM) 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and 0.2 - 1 mM (preferably 0.5 mM) N-hydroxysuccinimide (NHS); its dosage is calculated according to 20 - 40 mL (preferably 30 mL) per gram of freeze-dried powder of porcine esophageal mucosa acellular extracellular matrix.
[0010] Further, the stirring time in step 1) is 12 - 48 h (preferably 24 h).
[0011] Furthermore, in the mixed solution obtained in step 2), the mass percentage concentration of the porcine esophageal mucosa decellularized extracellular matrix adhesive is 2.5%-10% (preferably 7.5%), the mass percentage concentration of methacrylated hyaluronic acid is 0.5%-4% (preferably 2%), the mass percentage concentration of the photoinitiator is 0.25%-0.95% (preferably 0.75%), and the concentration of the anti-inflammatory drug is 3-9 mg / mL (preferably 6 mg / mL).
[0012] Furthermore, the anti-inflammatory drug is dexamethasone.
[0013] Furthermore, the photoinitiator is lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP).
[0014] Furthermore, the wavelength of the ultraviolet light used in step 3) is 365 nm, the power is 10 W, and the irradiation time is 10-40 min (preferably 30 min).
[0015] The obtained adhesive patch based on porcine esophageal mucosa decellularized extracellular matrix can not only effectively adhere to oral ulcers to promote the healing of oral ulcers, but also effectively reduce inflammation in oral mucosal tissues, and can therefore be used as a repair and therapeutic agent for oral ulcers and oral mucosal injuries.
[0016] Compared with the prior art, the main advantages of the present invention are: The adhesive patch based on the porcine esophageal mucosa decellularized extracellular matrix prepared by the present invention has good adhesive properties. At the same time, the patch can effectively utilize the active ingredients in the low-immunogenic decellularized esophageal mucosa extracellular matrix, such as collagen, glycosaminoglycans and elastin, to reduce the area of oral ulcers by promoting cell proliferation. In addition, the patch synergizes with anti-inflammatory drugs and can significantly promote the rapid healing of oral mucosal tissue damage and the disappearance of mucosal inflammation. BRIEF DESCRIPTION OF THE DRAWINGS
[0017] Figure 1 This is a histological staining comparison of the porcine esophageal mucosa decellularized extracellular matrix prepared in Example 1 and the original porcine esophageal mucosa tissue.
[0018] Figure 2 This is a comparison chart of the DNA, collagen and glycosaminoglycan contents of the porcine esophageal mucosa decellularized extracellular matrix freeze-dried powder prepared in Example 1 and the original porcine esophageal mucosa tissue.
[0019] Figure 3 This is a diagram showing the adhesion of the pig esophageal mucosa-based decellularized extracellular matrix adhesive patch prepared in Example 1 on different substrate surfaces.
[0020] Figure 4This is a diagram showing the adhesion of the pig esophageal mucosa-based decellularized extracellular matrix adhesive patch prepared in Example 1 in the rat oral cavity.
[0021] Figure 5 This is a comparison chart of the adhesion of different oral film products in Example 2 in the oral cavity of rats.
[0022] Figure 6 This is a diagram showing the oral ulcer lesion area of rats in different treatment groups at different treatment times in Example 4.
[0023] Figure 7 This is a quantitative analysis chart of the oral ulcer wound closure rate of rats in different treatment groups at different treatment times in Example 4.
[0024] Figure 8 This is a comparative diagram of CD11b immunofluorescence staining of oral mucosal lesions in rats in different treatment groups in Example 4.
[0025] Figure 9 This is a quantitative analysis of the mean fluorescence intensity of CD11b expression in the oral mucosal lesions of rats in different treatment groups in Example 4. DETAILED DESCRIPTION
[0026] An adhesive patch based on porcine esophageal mucosa decellularized extracellular matrix, the preparation method of which comprises the following steps: 1) The porcine esophageal mucosal tissue was peeled off from the porcine esophagus, placed in a mixed solution of methanol and chloroform (1:1, v / v) and stirred for 24 h for defatting, then treated with a 0.25% pancreatic enzyme solution containing 1 mM EDTA at 37°C for 6 h, and then treated with a 1% Triton solution containing 25 mM EDTA at 37°C for 24 h for decellularization, and then treated with a 30 U / mL DNase solution containing 10 mM magnesium chloride at 37°C for 24 h for DNA removal, washed and freeze-dried, and then digested in simulated gastric juice for 12 h, and then the pH of the digestive fluid was adjusted to 7, and then freeze-dried and ground again to obtain the porcine esophageal mucosal decellularized extracellular matrix freeze-dried powder; 2) The porcine esophageal mucosa decellularized extracellular matrix freeze-dried powder and the buffer solution containing dopamine hydrochloride were sealed and stirred at room temperature for 12-48 hours in an amount of 1g:20-40 mL, and then dialyzed and freeze-dried to obtain an adhesive based on the porcine esophageal mucosa decellularized extracellular matrix; 3) The adhesive based on the decellularized extracellular matrix of porcine esophageal mucosa obtained in step 2) and methacrylated hyaluronic acid are dissolved in deionized water, and the anti-inflammatory drug dexamethasone and the photoinitiator LAP are added in sequence under light-proof conditions, and stirred to obtain a mixed solution; in the obtained mixed solution, the mass percentage concentration of the decellularized extracellular matrix adhesive of porcine esophageal mucosa is 2.5%-10%, the mass percentage concentration of methacrylated hyaluronic acid is 0.5%-4%, the mass percentage concentration of the photoinitiator LAP is 0.25%-0.95%, and the concentration of dexamethasone is 3-9 mg / mL; 4) injecting the mixed solution obtained in step 3) into a polytetrafluoroethylene mold, irradiating with ultraviolet light of wavelength 365 nm and power 10 W for 10-40 min for curing, and then freeze-drying to obtain the oral ulcer adhesive patch based on porcine esophageal mucosa decellularized extracellular matrix.
[0027] Wherein, the buffer solution containing dopamine hydrochloride in step 1) is a MES buffer solution containing 0.5-3 mM dopamine hydrochloride, 0.2-1 mM EDC and 0.2-1 mM NHS.
[0028] In order to make the contents of the present invention easier to understand, the technical solution of the present invention is further described below in conjunction with specific implementation methods, but the present invention is not limited thereto.
[0029] Embodiment 1: 1) Fresh porcine esophageal tissue was washed, the porcine esophageal mucosal tissue was mechanically peeled off and minced, and then placed in a mixed solution of methanol and chloroform (1:1, v / v) and stirred for 24 h to obtain defatted porcine esophageal mucosal tissue.
[0030] 2) 80 g of the defatted porcine esophageal mucosal tissue was treated with 400 mL of 0.25% pancreatic enzyme solution (containing 1 mM EDTA) at 37°C for 6 h, 400 mL of 1% Triton solution (containing 25 mM EDTA) at 37°C for 24 h, and 400 mL of 30 U / mL DNA enzyme solution (containing 10 mM magnesium chloride) at 37°C for 24 h. Subsequently, the tissue was rinsed with PBS, washed with distilled water, sterilized with 75% ethanol, freeze-dried, and cryogenically ground to obtain porcine esophageal mucosal decellularized extracellular matrix.
[0031] 3) Take 10 g of the obtained porcine esophageal mucosa decellularized extracellular matrix and digest it with 1000 mL of simulated gastric juice containing 1 mg / mL pepsin and 0.5 M acetic acid at 37°C for 12 h, then adjust the pH of the digestive fluid to 7.0, and then freeze-dry and cryogenically grind to obtain the porcine esophageal mucosa decellularized extracellular matrix freeze-dried powder.
[0032] 4) 1 g of porcine esophageal mucosa decellularized extracellular matrix freeze-dried powder was mixed with 20 mL of MES buffer containing 2 mM dopamine hydrochloride, 0.5 mM EDC and 0.5 mM NHS and sealed and stirred at room temperature for 24 h. The adhesive based on porcine esophageal mucosa decellularized extracellular matrix was then obtained by dialyzing and freeze-drying.
[0033] 5) The adhesive based on the decellularized extracellular matrix of porcine esophageal mucosa and methacrylated hyaluronic acid were dissolved in ultrapure water, and then lithium phenyl-2,4,6-trimethylbenzoylphosphite (LAP) powder was added thereto under light-proof conditions, and the mixture was evenly mixed by magnetic stirring to obtain a mixed solution containing 7.5wt% of the adhesive based on the decellularized extracellular matrix of porcine esophageal mucosa, 2% of methacrylated hyaluronic acid, and 0.75wt% of LAP.
[0034] 6) The mixed solution was injected into a polytetrafluoroethylene mold and solidified by irradiation with ultraviolet light at a wavelength of 365 nm and a power of 10 W for 20 min. Finally, it was freeze-dried to obtain an adhesive patch based on the porcine esophageal mucosa decellularized extracellular matrix.
[0035] Performance Testing (1) Using the original porcine esophageal mucosal tissue as a control, the porcine esophageal mucosal decellularized extracellular matrix prepared in Example 1 was subjected to histological staining analysis. The results are as follows: Figure 1 shown.
[0036] Figure 1 H&E staining showed that the original porcine esophageal mucosal tissue had a clear nuclear structure and intact cell edges, while almost no nuclei were found in the porcine esophageal mucosal decellularized extracellular matrix prepared in Example 1, indicating that most cells and cell fragments had been effectively removed. Masson staining results showed that the porcine esophageal mucosal decellularized extracellular matrix prepared in Example 1 still retained a collagen fiber structure similar to that of natural esophageal mucosal tissue.
[0037] (2) Using the original porcine esophageal mucosal tissue as a control, the porcine esophageal mucosal decellularized extracellular matrix freeze-dried powder prepared in Example 1 was tested for DNA, collagen and glycosaminoglycan content. The results are as follows: Figure 2 shown.
[0038] Depend on Figure 2 It can be seen that compared with natural porcine esophageal mucosal tissue, the DNA content in the porcine esophageal mucosa decellularized extracellular matrix freeze-dried powder prepared in Example 1 is significantly reduced, and the dry weight ratio of collagen and glycosaminoglycan is increased.
[0039] (3) The adhesion performance of the adhesive patch based on the porcine esophageal mucosa decellularized extracellular matrix prepared in Example 1 was investigated. The results are as follows: Figure 3 shown.
[0040] likeFigure 3 As shown, the adhesive patch based on porcine esophageal mucosa decellularized extracellular matrix prepared in Example 1 can adhere to the surfaces of various materials and exhibits good adhesion properties.
[0041] like Figure 4 As shown, the adhesive patch based on the porcine esophageal mucosa decellularized extracellular matrix prepared in Example 1 can effectively adhere to various mucosal surfaces in the rat oral cavity.
[0042] Example 2 The adhesion performance of the adhesive patch based on the porcine esophageal mucosa decellularized extracellular matrix prepared in Example 1 in the rat oral cavity was evaluated by in situ adhesion photography. Three groups were set up in the experiment: control group 1 was a commercially available purple bamboo propolis oral film, control group 2 was a commercially available dexamethasone acetate oral patch, and the experimental group was the sample of Example 1. Under general anesthesia of the rats, the adhesion of each group of samples in the oral cavity was observed and recorded at 0, 0.5, 1, 1.5, 2, 3, and 4 hours, and photographed and recorded to evaluate the duration of adhesion. The results are shown in Figure 5 .
[0043] like Figure 5 As shown, the adhesive patch based on porcine esophageal mucosa decellularized extracellular matrix can continue to adhere in the rat oral cavity for 4 hours, and its adhesion time is significantly longer than that of control group 1 and control group 2, indicating that the adhesive patch based on porcine esophageal mucosa decellularized extracellular matrix has good adhesion properties in the rat oral environment.
[0044] Example 3 The steps for preparing the adhesive patch based on the decellularized extracellular matrix of porcine esophageal mucosa in this embodiment are basically the same as those in Example 1, except that in step 5) of this embodiment, the adhesive based on the decellularized extracellular matrix of porcine esophageal mucosa and methacrylated hyaluronic acid are dissolved in ultrapure water, and dexamethasone is added. Then, lithium phenyl-2,4,6-trimethylbenzoylphosphite (LAP) powder is added thereto under light-proof conditions, and the mixture is evenly mixed by magnetic stirring to obtain a mixed solution containing 7.5wt% of the adhesive based on the decellularized extracellular matrix of porcine esophageal mucosa, 2% of methacrylated hyaluronic acid, 0.75wt% of LAP, and 6mg / mL of dexamethasone.
[0045] Example 4 The acetic acid oral ulcer model experiment was used to evaluate the repair effect of the adhesive patch based on the decellularized extracellular matrix of porcine esophageal mucosa on the oral mucosa.
[0046] 1. Experimental Materials Commercially available dexamethasone acetate oral ulcer patch, the adhesive patch based on porcine esophageal mucosa decellularized extracellular matrix prepared in Example 1, the adhesive patch based on porcine esophageal mucosa decellularized extracellular matrix prepared in Example 3, 70% acetic acid, 4% paraformaldehyde.
[0047] 2. Experimental methods and results (1) Experimental groups There were 3 SD rats in each of the blank control group, model group, positive control group, Example 1 group, and Example 3 group.
[0048] (2) Group processing Blank control group (no treatment); model group (acetic acid injury); positive control group (acetic acid injury + dexamethasone acetate oral ulcer patch); Example 1 group (acetic acid injury + adhesion patch based on porcine esophageal mucosa decellularized extracellular matrix prepared in Example 1); Example 3 group (acetic acid injury + adhesion patch based on porcine esophageal mucosa decellularized extracellular matrix prepared in Example 3); the test samples used were administered once every two days.
[0049] (3) Experimental process After the rats were anesthetized, their mouths were cleaned with normal saline. Except for the blank control group, the other groups used 70% acetic acid solution to induce the oral ulcer model. The specific operation was as follows: the glass rod was soaked in 70% acetic acid solution for 3 minutes in advance, and then placed on the rat's mandibular gums for 60 seconds. After the induction was completed, the ulcer site was immediately rinsed with normal saline to completely remove the residual acetic acid. After 2 consecutive days of observation, when obvious white ulcers appeared in the rat's mouth, it was recorded as the 0th day of treatment. The rats were grouped and treated from day 0 for 8 days. After the treatment, the rats were euthanized and the damaged oral tissues were collected for various index tests.
[0050] (4) Evaluation of oral mucosal repair in rats The oral ulcer sites of rats in each group were photographed and recorded. Figure 6 At the same time, Image J software was used to analyze the ulcer area, and the ulcer area on day 0 was used as the benchmark to calculate the oral mucosal ulcer wound closure rate (oral mucosal ulcer wound closure rate = 1-ulcer area on day n / ulcer area on day 0). The results are shown in Figure 7 shown.
[0051] Depend on Figure 6 , 7 The experimental results showed that the positive control group, Example 1 group and Example 3 group all showed significant oral mucosal repair effects. After 8 days of treatment, the morphological characteristics of the oral mucosa of Example 3 group were closest to normal tissues, and its ulcer wound closure rate reached 96.10%, which was significantly higher than 69.23% of the model group (p<0.001), and was also better than Example 1 group. These results show that the adhesive patch based on the decellularized extracellular matrix of porcine esophageal mucosa has a significant repair effect on acetic acid-induced oral mucosal damage, among which Example 3 showed the best therapeutic effect.
[0052] (5) Determination of inflammatory cell expression in rat oral mucosal tissue The damaged oral mucosal tissues of rats in each group were sliced and CD11b was labeled with CD11b antibody. + Cells (including monocytes, neutrophils, natural killer cells, granulocytes, macrophages and other white blood cell subsets) to assess the degree of inflammatory response. The results of fluorescent staining are as follows Figure 8 The mean fluorescence intensity of each group was analyzed using ImageJ software, and the quantitative analysis results were shown in Figure 9 shown.
[0053] Depend on Figure 8 , 9 The results showed that compared with the blank control group, the model group CD11b + Cell infiltration increased significantly (p<0.001). After 8 days of treatment, the positive control group, Example 1 group and Example 3 group were able to significantly reduce CD11b + The number of cells was significantly reduced (p<0.001), and the inflammatory cells in the Example 3 group were the most significantly reduced. These results show that the adhesive patch based on the porcine esophageal mucosa decellularized extracellular matrix can effectively inhibit the recruitment of inflammatory cells, promote the disappearance of inflammatory reactions around the damaged oral mucosa, and make the tissue state tend to normal, thereby facilitating the repair process of oral mucosal damage.
[0054] The above description is only a preferred embodiment of the present invention. All equivalent changes and modifications made according to the scope of the patent application of the present invention should fall within the scope of the present invention.
Claims
1. A method for preparing an adhesive patch based on porcine esophageal mucosa decellularized extracellular matrix, characterized in that: The following steps are involved: 1) The porcine esophageal mucosa decellularized extracellular matrix freeze-dried powder and the buffer solution containing dopamine hydrochloride were sealed and stirred at room temperature, and then dialyzed and freeze-dried to obtain an adhesive based on the porcine esophageal mucosa decellularized extracellular matrix; 2) dissolving the adhesive based on porcine esophageal mucosa decellularized extracellular matrix obtained in step 1) and methacrylated hyaluronic acid in deionized water, adding anti-inflammatory drugs and photoinitiators in sequence under light-proof conditions, and stirring to obtain a mixed solution; 3) injecting the mixed solution obtained in step 2) into a mold, curing it by ultraviolet light, and then freeze-drying it to obtain the oral ulcer adhesive patch based on porcine esophageal mucosa decellularized extracellular matrix.
2. The preparation method according to claim 1, characterized in that: Step 1) The preparation of the pig esophageal mucosa decellularized extracellular matrix freeze-dried powder is to peel the pig esophageal mucosa tissue from the pig esophagus, and then perform defatting, decellularization, DNA removal and simulated gastric juice digestion treatment, adjust the pH of the digestive juice to 7, and freeze-dry and grind it again.
3. The preparation method according to claim 1 or 2, characterized in that The DNA content in the porcine esophageal mucosa decellularized extracellular matrix freeze-dried powder is less than 50 ng / mg.
4. The preparation method according to claim 1, characterized in that The buffer solution containing dopamine hydrochloride in step 1) is a MES buffer solution containing 0.5-3 mM dopamine hydrochloride, 0.2-1 mM EDC and 0.2-1 mM NHS; the amount thereof is converted to 20-40 mL per gram of porcine esophageal mucosal decellularized extracellular matrix lyophilized powder.
5. The preparation method according to claim 1, characterized in that: The stirring time in step 1) is 12-48 hours.
6. The preparation method according to claim 1, characterized in that Step 2) In the obtained mixed solution, the mass percentage concentration of the porcine esophageal mucosa decellularized extracellular matrix adhesive is 2.5%-10%, the mass percentage concentration of methacrylated hyaluronic acid is 0.5%-4%, the mass percentage concentration of the photoinitiator is 0.25%-0.95%, and the concentration of the anti-inflammatory drug is 3-9 mg / mL.
7. The preparation method according to claim 1 or 6, characterized in that: The anti-inflammatory drug is dexamethasone, and the photoinitiator is lithium phenyl-2,4,6-trimethylbenzoylphosphinate.
8. The preparation method according to claim 1, characterized in that Step 3) The wavelength of the ultraviolet light used is 365 nm, the power is 10 W, and the irradiation time is 10-40 min.
9. An adhesive patch based on porcine esophageal mucosa decellularized extracellular matrix prepared by the method as claimed in claim 1.
10. Use of the adhesive patch based on porcine esophageal mucosa decellularized extracellular matrix as claimed in claim 9 as a therapeutic agent for oral ulcers.