Biological fermentation feed as well as preparation method and application thereof

Abstract

The invention discloses biological fermentation feed and a preparation method and application thereof, and belongs to the technical field of feed. In order to solve the technical problems of difficult feeding, easy mildewing and unstable quality of the fermented feed in the prior art, the invention provides a biological fermented feed, which comprises 6% of a biological agent, 35% of corn germ meal, 10% of crushed corn, 40% of starch residue and 9% of glucose mother liquor. The biological fermentation feed provided by the invention has relatively low water content and rich bioactive substances, is stable in quality after being mixed with common feed in the market, and has the obvious advantage of being easy to store; the biological fermentation feed has a remarkable weight increasing effect when being used for feeding cattle and pigs, can reduce the feed conversion ratio and the money conversion ratio, and achieves the purposes of reducing the feed cost and increasing the weight increasing income.

Description

Technical Field

[0001] The present invention belongs to the technical field of feeds, and relates to a biological fermented feed, a preparation method thereof and an application thereof. Background Art

[0002] In recent years, fermented biological feeds have been popular among farmers because they have significant advantages in terms of function and cost; the functionality of fermented feeds lies in that the microorganisms in them produce five types of nutrients essential for animal health and nutrition during the fermentation process, including: organic acids, bacteriocins, digestive enzymes, vitamins, and amino acids; in addition, fermented feeds also contain a large number of probiotics. After animals ingest them and enter the gastrointestinal tract, some of them colonize on the mucosal surface, increasing the density of the protective film, which can prevent the colonization and invasion of a large number of pathogenic bacteria, balance the intestinal microecology, and improve intestinal health. The fermented feed contains a large number of live probiotic bacteria, which can effectively improve the activity of host macrophages, produce a variety of immune factors, and enhance the body's immunity; the probiotics contained in the fermented feed can produce a large number of digestive enzymes in vitro, playing a role in the pretreatment of feed raw materials; and during the fermentation process, the large amount of organic acids produced by the metabolism of probiotics using feed raw materials can play a role in inhibiting the growth of pathogenic bacteria, and the antibacterial substances such as bacteriocins produced can effectively inhibit the reproduction of pathogenic bacteria.

[0003] At present, for the research and development of fermented feeds, in addition to requiring excellent feed quality and rich nutrients, the requirements for the quality and safety during their storage period are also getting higher and higher. There is a risk of mold infection in all links of fermented feed production, processing, and storage; mildew can lead to a decrease in the nutritional value of the feed, a decline in palatability, and animal poisoning. From the estimated data of the Food and Agriculture Organization, it can be seen that about 5% - 7% of feeds or feed raw materials have quality problems due to mold infestation every year, resulting in serious economic losses.

[0004] Therefore, those skilled in the art are eager to develop a fermented feed with anti - mildew characteristics. To prevent the occurrence of mildew in fermented feeds, the quality of feed raw materials should be strengthened, and the water content of the raw materials is required to be within a safe range. The water in the feed can be divided into free water and bound water. When the water content of most feeds exceeds 11.5%, a part of free water will appear. When the water content exceeds 13% - 14%, a large number of molds will grow and reproduce. When the water content exceeds 15%, the growth rate of molds will increase significantly.

[0005] The entry of biological feeds into the feed production end is the future development trend. Since conventional wet fermented feeds have problems such as difficult feeding, easy mildew, and unstable quality as functional raw materials in the feed production end, they are not suitable for use in the feed production end. Developing functional low - moisture products will make up for these drawbacks, be convenient to use, and products with reasonable cost performance and good functionality will become the first choice of feed mills. Summary of the Invention

[0006] In order to solve the technical problems of difficult feeding, easy mildew, and unstable quality existing in the existing fermented feed in the prior art, the present invention provides a biological fermented feed, a preparation method thereof, and an application thereof.

[0007] One of the purposes of the present invention is to provide a biological fermented feed, and the fermented feed includes: 6% biological bacterial agent, 35% corn germ meal, 10% crushed corn, 40% starch residue, and 9% glucose mother liquor.

[0008] In a preferred embodiment of the present invention, the biological bacterial agent is prepared by mixing a bacterial strain fermentation broth and an enzyme preparation, and the preparation method of the biological bacterial agent is: adding 15 - 50 g of cellulase, 20 - 60 g of protease, and 40 - 80 g of glucanase to every 60 kg of the bacterial strain fermentation broth.

[0009] In a preferred embodiment of the present invention, the bacterial strain fermentation broth is prepared by mixing a Lactobacillus plantarum fermentation broth, a Candida utilis fermentation broth, and a Bacillus subtilis + Bacillus licheniformis mixed fermentation broth according to a mixing volume ratio of 1:2:1.

[0010] In a preferred embodiment of the present invention, the preservation number of the Lactobacillus plantarum is CGMCC 1.12934, the preservation number of the Candida utilis is CGMCC 2.2951, the preservation number of the Bacillus subtilis is CGMCC 1.15792, and the preservation number of the Bacillus licheniformis is CGMCC 1.10257.

[0011] In a preferred embodiment of the present invention, the cellulase is 20000 U / g, the protease is 50000 U / , and the glucanase is 10000 U / g.

[0012] Another purpose of the present invention is to provide a preparation method of the above biological fermented feed, and the preparation method includes the following steps:

[0013] S1: Separately conduct liquid fermentation on Lactobacillus plantarum and Candida utilis, conduct mixed liquid fermentation on Bacillus subtilis and Bacillus licheniformis, and mix the obtained fermentation broths according to a mixing volume ratio of 1:2:1 for the first time to obtain a bacterial strain fermentation broth;

[0014] S2: Add 15 - 50 g of cellulase, 20 - 60 g of protease, and 40 - 80 g of glucanase to every 60 kg of the bacterial strain fermentation broth obtained in S1 to obtain a biological bacterial agent;

[0015] S3: Mix the biological bacterial agent obtained in S2 with corn germ meal, broken corn, starch residue, and glucose mother liquor according to the mass percentages of 6%: 35%: 10%: 40%: 9%, place them in a mixer, stir for 5 min, pack, with each pack being 0.5 tons, place them in a fermentation room at ≥25 °C for secondary micro-anaerobic fermentation for 5 - 7 days, dry at 50 - 60 °C until the water content < 10%, crush, package, and store to obtain the biological fermentation feed;

[0016] The storage conditions are: temperature 25 °C, relative humidity < 60%.

[0017] In a preferred embodiment of the present invention, the preparation steps of the Lactobacillus plantarum fermentation broth in S1 are: Under aseptic operation conditions, inoculate the slant Lactobacillus plantarum CGMCC 1.12934 into the sterilized MRS liquid medium, 1 L per bottle, a total of 7 bottles, place them at 37 °C for static culture for 48 h, combine the bottles, and then inoculate them into a sterilized 50 L fermenter at 37 °C filled with the modified MRS medium for anaerobic stirring culture for 48 h;

[0018] The composition of the modified MRS medium is: 10 g peptone, 10 g beef extract, 5 g yeast extract, 2 g dipotassium hydrogen phosphate, 2 g diammonium citrate, 5 g sodium acetate, 30 g glucose mother liquor, 1 mL Tween 80, 0.5 g magnesium sulfate, 0.25 g manganese sulfate, and the balance is water, configured into 1 L of the modified medium solution.

[0019] In a preferred embodiment of the present invention, the preparation steps of the Candida utilis fermentation broth in S1 are: Under aseptic operation conditions, inoculate Candida utilis CGMCC 2.2951 into the sterilized yeast liquid medium, 1 L per bottle, a total of 10 bottles, place them in a 28 °C air bath shaker for culture for 24 h, combine the bottles, and then inoculate them into a sterilized 100 L fermenter for aerobic culture for 24 h.

[0020] In a preferred embodiment of the present invention, the preparation steps of the mixed fermentation broth of Bacillus subtilis and Bacillus licheniformis in S1 are: Under aseptic operation conditions, inoculate Bacillus subtilis CGMCC 1.15792 and Bacillus licheniformis CGMCC 1.10257 into the slant of the sterilized LB medium in eggplant bottles, 5 bottles each, place them at 37 °C for culture for 16 h; after culture, elute with sterilized distilled water, and then inoculate them into a 50 L fermenter for aerobic culture for 24 h.

[0021] The third object of the present invention is to provide the application of the above biological fermentation feed in improving the weight gain effect of feeding cattle and pigs.

[0022] Advantages of the present invention: The present invention provides a biologically fermented feed, which comprises a biological bacterium agent, corn germ meal, crushed corn, starch residue and glucose mother liquor, and their mixing mass percentages are 6%: 35%: 10%: 40%: 9%. The present invention performs mixed fermentation on Bacillus licheniformis and Bacillus subtilis, promotes the large production of 5 kinds of biological enzymes by using the flora effect among the strains, synergistically promotes the accumulation of digestive enzyme products, and realizes the improvement of the digestibility of the fermented feed by means of secondary fermentation. The biologically fermented feed provided by the present invention has a low water content, contains rich bioactive substances, is stable in quality after being mixed with ordinary feeds on the market, has an obvious advantage of being easy to store, can effectively solve the technical problem of difficult feed feeding, and at the same time solve the technical problems of easy mildew and short storage period after feed mixing.

[0023] The biologically fermented feed provided by the present invention has a significant weight gain effect when feeding cattle and pigs, can reduce the feed-to-meat ratio and money-to-meat ratio, and realizes the purpose of reducing feed cost and increasing weight gain income. Detailed implementation manners

[0024] Those skilled in the art can draw on the content of this article and appropriately improve the process parameters to achieve. It should be particularly noted that all similar substitutions and modifications are obvious to those skilled in the art, and they are all regarded as included in the present invention. The methods and applications of the present invention have been described through preferred embodiments, and those related can obviously make changes or appropriate changes and combinations to the methods and applications described herein without departing from the content and scope of the present invention to implement and apply the technology of the present invention.

[0025] In order to make the purpose, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with specific implementation manners. The experimental methods used in the following examples are all conventional methods unless otherwise specified, and the materials, reagents, methods and instruments used, unless otherwise specified, are all conventional materials, reagents, methods and instruments in this field, and those skilled in the art can obtain them through commercial channels.

[0026] In the following examples, the preservation number of Lactobacillus plantarum is CGMCC 1.12934, the preservation number of Candida utilis is CGMCC 2.2951, the preservation number of Bacillus subtilis is CGMCC 1.15792, and the preservation number of Bacillus licheniformis is CGMCC 1.10257; the above-mentioned Lactobacillus plantarum, Candida utilis, Bacillus subtilis and Bacillus licheniformis are all purchased from the General Microbiological Center of the China Committee for Culture Collection of Microorganisms.

[0027] Example 1: Preparation of a biologically fermented feed

[0028] S1: Conduct separate liquid fermentations on Lactobacillus plantarum and Candida utilis respectively, and conduct a mixed liquid fermentation on Bacillus subtilis and Bacillus licheniformis. Mix the obtained fermentation broths according to a mixed volume ratio of 1:2:1 to obtain a bacterial strain fermentation broth.

[0029] S2: Add 25 g of cellulase, 30 g of protease, and 60 g of glucanase to every 60 kg of the bacterial strain fermentation broth obtained in S1 to obtain a biological bacterial agent. The cellulase: 20000 U / g, protease: 50000 U / g, and glucanase: 10000 U / g are all from the screening and preservation of Xiangbai Biotechnology Co., Ltd.

[0030] S3: Mix the biological bacterial agent obtained in S2 with corn germ meal, broken corn, starch residue, and glucose mother liquor according to a mass percentage of 6%: 35%: 10%: 40%: 9%, place them in a mixer, stir for 5 min, pack, 0.5 tons per package, place them in a fermentation room not lower than 25 °C for secondary micro-anaerobic fermentation for 5 - 7 days, dry at 50 - 60 °C until the water content is less than 10%, crush, and use double-layer packaging. The outer layer is a composite woven bag, and the inner layer is a plastic bag, with a net content of 25 kg per bag to obtain a biological fermentation feed. The fermentation feed is stored under the conditions of a storage temperature of 25 °C and a relative humidity of less than 60%.

[0031] The preparation steps of the Lactobacillus plantarum fermentation broth described in S1 are as follows: Under aseptic operation conditions, inoculate the slant Lactobacillus plantarum CGMCC 1.12934 into the sterilized MRS liquid medium (the composition of the MRS medium is: 10 g of peptone, 10 g of beef extract, 5 g of yeast extract, 2 g of dipotassium hydrogen phosphate, 2 g of diammonium citrate, 5 g of sodium acetate, 20 g of glucose, 1 mL of Tween 80, 0.5 g of magnesium sulfate, 0.25 g of manganese sulfate, and the balance is water, configured into 1 L of MRS medium solution), a total of 7 bottles, 1 L per bottle, place them at 37 °C for static culture for 48 hours. After combining the bottles, inoculate them into a 50 L fermentation tank at 37 °C filled with the modified medium (the composition of the modified medium is: 10 g of peptone, 10 g of beef extract, 5 g of yeast extract, 2 g of dipotassium hydrogen phosphate, 2 g of diammonium citrate, 5 g of sodium acetate, 30 g of glucose mother liquor, 1 mL of Tween 80, 0.5 g of magnesium sulfate, 0.25 g of manganese sulfate, and the balance is water, configured into 1 L of modified medium solution) for anaerobic stirring culture for 48 hours.

[0032] The preparation steps of the Candida utilis fermentation broth described in S1 are as follows: Under aseptic conditions, inoculate Candida utilis CGMCC 2.2951 into the sterilized yeast liquid medium (the composition of the yeast liquid medium is: 1% yeast extract, 2% peptone, 3% glucose mother liquor), a total of 10 bottles, 1 L per bottle, and culture in a shaking water bath at 28°C for 24 h. After combining the bottles, inoculate them into a sterilized 100-L fermenter and perform aerobic culture for 24 hours;

[0033] The preparation steps of the mixed fermentation broth of Bacillus subtilis and Bacillus licheniformis described in S1 are as follows: Under aseptic conditions, inoculate Bacillus subtilis CGMCC 1.15792 and Bacillus licheniformis CGMCC 1.10257 into the slant of the eggplant bottle of the sterilized LB medium, 5 bottles each, and culture at 37°C for 16 h; After culturing, elute with sterilized distilled water, and then inoculate into a 50-L fermenter and perform aerobic culture for 24 hours.

[0034] Effect experiment:

[0035] (1) Detection of biological enzyme activity

[0036] In this example, the fermentation feed was prepared by the mixed fermentation method of Bacillus licheniformis and Bacillus subtilis. To explore the effects of single fermentation and mixed fermentation, Bacillus subtilis CGMCC 1.15792 and Bacillus licheniformis CGMCC 1.10257 were separately subjected to liquid fermentation under the same conditions as the mixed liquid fermentation in Example 1; and the fermentation broths obtained by the above 3 different fermentation methods were detected for 5 kinds of biological enzyme activities.

[0037] The detection method of protease activity refers to the determination methods of acidic, neutral, and alkaline proteases in GB / T 23527-2009 to detect the total protease activity;

[0038] The detection method of amylase refers to GB / T 24401-2009 to detect the total amylase activity;

[0039] The detection method of pectinase can refer to Enzyme Preparations for Food Industry (GB1886.174-2016);

[0040] The detection method of cellulase refers to GB / T 23881-2009;

[0041] The detection method of lipase refers to GB / T 23535-2009.

[0042] The detection results are shown in Table 1. In the mixed fermentation broth obtained by the mixed fermentation of Bacillus licheniformis and Bacillus subtilis, the activities of 5 kinds of biological enzymes are significantly higher than those in the fermentation broth obtained by the independent fermentation of Bacillus licheniformis or Bacillus subtilis. Among them, the protease content, amylase content, pectinase activity, and cellulase activity in the mixed fermentation broth are significantly higher than those in the fermentation broth of Bacillus licheniformis alone, while the lipase activity has no significant difference. The protease content, amylase content, cellulase, and lipase activities in the mixed fermentation broth are significantly higher than those in the fermentation broth of Bacillus subtilis alone, while the pectinase activity has no significant difference.

[0043] It can be seen that the flora effect occurring during the mixed fermentation of Bacillus licheniformis and Bacillus subtilis promotes the large production of 5 kinds of biological enzymes. The biological enzymes can degrade the macromolecular substances in the fermentation raw materials and degrade them into small molecular substances that are easily absorbed by animals. It can be seen that the higher the biological enzyme activity, the more important it plays in the subsequent secondary fermentation.

[0044] Table 1

[0045]

[0046]

[0047] (2) Detection of nutritional indicators

[0048] The present invention detects the nutritional indicators of the fermented feed prepared in Example 1. The detection results are shown in Table 2. All the indicators of the biological fermented feed provided by the present invention meet the feed standards. Among them, the total amino acid accounts for 94.34% of the crude protein content, and the absorbable utilization rate is relatively high. The moisture content is 10%, which is much lower than the safety critical value (11.5%), and it is not easy to mildew during the storage period. It can be seen that the biological fermented feed provided by the present invention has a low water content, is rich in bioactive substances, and has a stable quality after being mixed with ordinary feeds on the market, and has obvious advantages in easy storage.

[0049] Table 2

[0050] Test Items Content Total Amino Acids (%) 15.99 Crude Protein (%) 16.95 Moisture (%) 10 Crude Fiber (%) 3.4 Crude Ash (%) 5 Total Phosphorus (%) 0.79 Calcium (%) ＜0.05 Neutral Detergent Fiber (%) 19.82 Acid Detergent Fiber (%) 5.99 Zearalenone (μg / kg) 77.4 Aflatoxin B1 (μg / kg) ＜2 Vomitoxin (g / kg) 2.7

[0051] (3) Detection of anti-mildew effect

[0052] The present invention sets up 3 experimental groups, which are respectively:

[0053] Experimental group 1: 10% of the biological fermented feed prepared in Example 1 + 90% of ordinary feed;

[0054] Experimental group 2: 5% of wet fermented material + 95% of ordinary feed;

[0055] Experimental group 3: Ordinary feed.

[0056] The initial moisture content of the feed in the above experimental groups 1-3 was detected in this invention. Under the condition that the temperature in the warehouse was stable at room temperature in summer and the relative humidity was 60%, the moisture content and total mold count of the feed in Examples 1-3 were detected under the same storage time; for the detection method of the total mold count, refer to the National Standard of the People's Republic of China GB / T13092-2006 "Determination of Total Mold Count in Feed".

[0057] The detection results are shown in Table 3. As the storage period extended, the moisture content and total mold count of the feed in Experimental Group 1 were lower than those in Experimental Groups 2-3. And when stored for 3 months, the total mold count was relatively stable. While for the feed in Experimental Group 2, mildew spots appeared after 1 month of storage. Although the detected total mold count was not high, there was already a mildew smell. From this, it can be shown that the bio-fermented feed provided by this invention has a significant anti-mildew effect compared with the wet fermented feed.

[0058] Table 3

[0059]

[0060] Comparative Example 1:

[0061] The difference between this comparative example and Example 1 is that in S1, Lactobacillus plantarum, Candida utilis, Bacillus subtilis and Bacillus licheniformis were separately subjected to liquid fermentation, and the obtained fermentation broths were mixed. The mixing volume ratio of the Bacillus licheniformis fermentation broth: Bacillus subtilis fermentation broth: Lactobacillus plantarum fermentation broth: Candida utilis fermentation broth was 0.5:0.5:1:2, and other steps were the same as those in Example 1.

[0062] Comparative Example 2:

[0063] The difference between this comparative example and Example 1 is that in S1, Lactobacillus plantarum, Candida utilis, Bacillus subtilis and Bacillus licheniformis were separately subjected to liquid fermentation, and the obtained fermentation broths were mixed. The mixing volume ratio of the Bacillus licheniformis fermentation broth: Bacillus subtilis fermentation broth: Lactobacillus plantarum fermentation broth: Candida utilis fermentation broth was 0.4:0.6:1:2, and other steps were the same as those in Example 1.

[0064] Comparative Example 3:

[0065] The difference between this comparative example and Example 1 is that in S1, Lactobacillus plantarum, Candida utilis, Bacillus subtilis and Bacillus licheniformis were separately subjected to liquid fermentation, and the obtained fermentation broths were mixed. The mixing volume ratio of the Bacillus licheniformis fermentation broth: Bacillus subtilis fermentation broth: Lactobacillus plantarum fermentation broth: Candida utilis fermentation broth was 0.6:0.4:1:2, and other steps were the same as those in Example 1.

[0066] Effect experiment:

[0067] The present invention conducts a bionic digestion experiment on the fermented feeds prepared in Example 1 and Comparative Examples 1-3 to evaluate the digestibility of the fermented feeds. 1 kg of each of the fermented feeds prepared in Example 1 and Comparative Examples 1-3 is placed in an incubator at a constant temperature of 30°C for 3 days. The fermented feeds are taken out, dried at 55°C respectively, and the drying is stopped after the moisture content is measured to be less than 10%. The pulverization fineness is 60 mesh. 2.0000 g is accurately weighed respectively, and 5 bottles are weighed for each kind of feed. Each time, 2 feeds are used, and a total of 2 in vitro bionic digestion tests are carried out according to the operation process of a monogastric animal bionic digester. After the test, the remaining residues are dried and accurately weighed to 4 decimal places, and the average value is obtained through comparative analysis.

[0068] As shown in Table 4, the digestibility of the fermented feed prepared in the example of the present invention is significantly higher than that of the fermented feeds prepared in Comparative Examples 1-3. It can be seen that Bacillus subtilis CGMCC 1.15792 and Bacillus licheniformis CGMCC 1.10257 synergistically promote the accumulation of digestive enzyme products during the mixed liquid fermentation process, thereby achieving an improvement in the digestibility of the fermented feed.

[0069] Table 4

[0070] Feed Name Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Digestibility 78.31％ 71.35％ 74.69％ 72.45％

[0071] Example 2: Application of biological fermented feed in improving the weight gain effect of cattle and pigs

[0072] (1) In this example, 190 pigs of the same batch are randomly divided into Experimental Group 1 and Control Group 1, with 95 pigs in each group. The feed formulations of the fattening pigs in Experimental Group 1 and Control Group 1 are shown in Table 5. The feeding period is 28 days, and the body weight, feed-to-meat ratio, money-to-meat ratio, number of diarrhea pigs, diarrhea rate, and mortality rate of the fattening pigs before and after feeding are statistically analyzed.

[0073] As shown in Table 6, the weight gain effect of the fattening pigs fed with 10% of the biological fermented feed provided by the present invention (Experimental Group 1) is obvious, and the feed-to-meat ratio and money-to-meat ratio are significantly reduced; moreover, in terms of feed cost, Experimental Group 1 reduces the feeding cost compared with Control Group 1.

[0074] Table 5

[0075]

[0076]

[0077] Table 6

[0078]

[0079] (2) In this example, 184 pigs from 20 litters in 2 farrowing houses of the same batch were selected and randomly divided into experimental group 2 and control group 2, with 92 pigs in each group. The feed formulations for the fattening pigs in experimental group 2 and control group 2 are shown in Table 7. The feeding period was 28 days, and the body weight, feed-to-meat ratio, money-to-meat ratio, number of diarrhea cases, diarrhea rate, and mortality rate of the fattening pigs before and after feeding were statistically analyzed.

[0080] The statistical results are shown in Table 8. The fattening pigs fed with the bio-fermented feed (experimental group 2) provided by the present invention at 20% had obvious weight gain effects, and the feed-to-meat ratio and money-to-meat ratio were significantly reduced. Moreover, in terms of feed cost, experimental group 2 reduced the feeding cost compared with control group 2.

[0081] Table 7

[0082]

[0083] Table 8

[0084]

[0085]

[0086] (3) In this example, 80 cows with a body weight of 400 kg each were selected and randomly divided into experimental group 3 and control group 3, with 40 cows in each group. The feed formulations for the fattening cows in experimental group 3 and control group 3 are shown in Table 9. The feeding period was 30 days, and the body weight, feed-to-meat ratio, and money-to-meat ratio of the fattening cows before and after feeding were statistically analyzed.

[0087] The statistical results are shown in Table 10. The fattening cows fed with the bio-fermented feed (experimental group 3) provided by the present invention at 25% had obvious weight gain effects, and the feed-to-meat ratio and money-to-meat ratio were significantly reduced. Moreover, in terms of feed cost, experimental group 3 reduced the feeding cost compared with control group 3.

[0088] Table 9

[0089]

[0090] Table 10

[0091]

[0092] (4) In this example, 84 cows with a body weight of 200 kg each were selected and randomly divided into experimental group 4 and control group 4, with 42 cows in each group. The feed formulations for the fattening cows in experimental group 4 and control group 4 are shown in Table 11. The feeding period was 30 days, and the body weight, feed-to-meat ratio, and money-to-meat ratio of the fattening cows before and after feeding were statistically analyzed.

[0093] The statistical results are shown in Table 12. The weight gain effect of the fattening cattle fed with the biological fermented feed (Experimental Group 4) provided by the present invention containing 50% is obvious, and the feed-to-meat ratio and cost-to-meat ratio are significantly reduced. Moreover, in terms of feed cost, Experimental Group 4 reduces the feeding cost compared with Control Group 4.

[0094] Table 11

[0095]

[0096] Table 12

[0097]

[0098] In summary, the biological fermented feed provided by the present invention has a significant weight gain effect when feeding cattle and pigs, and can reduce the feed-to-meat ratio and cost-to-meat ratio, so as to achieve the purpose of reducing feed cost and increasing weight gain benefits.

[0099] The content not described in detail in the specification of the present invention is well-known technology to those skilled in the art. Although the present invention has been disclosed above with preferred embodiments, it is not intended to limit the present invention. Anyone familiar with this technology can make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, the protection scope of the present invention should be defined by the claims.

Claims

1. A biological fermentation feed, characterized in that: The fermented feed comprises: 6% biological bacteria agent, 35% corn germ meal, 10% crushed corn, 40% starch residue and 9% glucose mother solution.

2. The fermented feed according to claim 1, characterized in that: The biological agent is prepared by mixing bacterial fermentation liquid and enzyme preparation. The preparation method of the biological agent is as follows: 15-50g of cellulase, 20-60g of protease and 40-80g of glucanase are added to every 60kg of bacterial fermentation liquid.

3. The fermented feed according to claim 2, characterized in that: The bacterial fermentation liquid is prepared by mixing Lactobacillus plantarum fermentation liquid, Candida utilis fermentation liquid, and Bacillus subtilis + Bacillus licheniformis mixed fermentation liquid in a mixing volume ratio of 1:2:

1.

4. The fermented feed according to claim 3, characterized in that The accession number of the Lactobacillus plantarum is CGMCC1.12934, the accession number of the Candida utilis is CGMCC 2.2951, the accession number of the Bacillus subtilis is CGMCC 1.15792, and the accession number of the Bacillus licheniformis is CGMCC 1.10257.

5. The fermented feed according to claim 2, characterized in that: The cellulase is 20000U / g, the protease is 50000U / g, and the glucanase is 10000U / g.

6. The method for preparing the bio-fermented feed according to any one of claims 1 to 5, characterized in that: The preparation method comprises the following steps: S1: performing liquid fermentation on Lactobacillus plantarum and Candida utilis separately, performing mixed liquid fermentation on Bacillus subtilis and Bacillus licheniformis, and mixing the fermentation broths obtained above once at a mixing volume ratio of 1:2:1 to obtain a bacterial fermentation broth; S2: adding 15-50 g of cellulase, 20-60 g of protease and 40-80 g of glucanase to every 60 kg of the fermentation liquid of the strain obtained in S1 to obtain a biological agent; S3: the biological agent obtained in S2 is mixed with corn germ meal, crushed corn, starch residue and glucose mother solution in a mass percentage of 6%:35%:10%:40%:9%, and then placed in a mixer, stirred for 5 minutes, packaged, each package is 0.5 tons, placed in a fermentation room at ≥25°C for secondary micro-anaerobic fermentation for 5-7 days, dried at 50-60°C to a moisture content of <10%, crushed, packaged, stored, and biological fermented feed is obtained; The storage conditions are: temperature 25° C., relative humidity < 60%.

7. The preparation method according to claim 6, characterized in that: The preparation steps of the Lactobacillus plantarum fermentation broth in S1 are as follows: under aseptic operation conditions, inoculate the slant Lactobacillus plantarum CGMCC 1.12934 into a sterilized MRS liquid culture medium, 1 L per bottle, a total of 7 bottles, place at 37°C for static culture for 48 hours, close the bottles, and then inoculate into a sterilized 50L fermentation tank filled with a modified MRS culture medium at 37°C, and culture for 48 hours with anaerobism and stirring; The modified MRS culture medium is composed of: 10 g peptone, 10 g beef extract, 5 g yeast extract, 2 g dipotassium hydrogen phosphate, 2 g diammonium citrate, 5 g sodium acetate, 30 g glucose mother solution, 1 mL Tween 80, 0.5 g magnesium sulfate, 0.25 g manganese sulfate, and the balance is water, which is configured into 1 L of modified culture medium solution.

8. The preparation method according to claim 6, characterized in that: The preparation steps of the fermentation broth of Candida utilis described in S1 are as follows: under aseptic operation conditions, Candida utilis CGMCC 2.2951 is inoculated into the sterilized yeast liquid culture medium, 1 L per bottle, a total of 10 bottles, placed in a 28°C air bath shaker for 24 hours, the bottles are closed, and then inoculated into a sterilized 100L fermenter, and aerobically cultured for 24 hours.

9. The preparation method according to claim 6, characterized in that: The preparation steps of the mixed fermentation liquid of Bacillus subtilis and Bacillus licheniformis described in S1 are as follows: under aseptic operation conditions, Bacillus subtilis CGMCC 1.15792 and Bacillus licheniformis CGMCC 1.10257 are inoculated into the slant of the sterilized LB medium eggplant bottle, 5 bottles each, and cultured at 37°C for 16 hours; after culture, the mixture is eluted with sterile distilled water, and then inoculated into a 50L fermenter, and cultured aerobically for 24 hours.

10. Use of the bio-fermented feed according to any one of claims 1 to 5 in feeding cattle and pigs to improve the weight gain of cattle and pigs.

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