Anti-hair loss essence and preparation method thereof

Abstract

The present application belongs to the field of biotechnology. More particularly, it relates to a hair loss prevention serum and a preparation method thereof. The hair loss prevention serum comprises the following components in mass fraction: a hair loss prevention composition 1-3%, a humectant 2-5%, a solubilizer 1-5%, a thickening agent 0.1-1%, a hair conditioner 1-3%, a soothing agent 1-3%, a pH regulator 0.1-1%, and a solvent in the remaining amount; and the hair loss prevention composition is composed of psoralen and 6-pyrrolidinyl-2,4-diaminopyrimidine-3-oxide in a weight ratio of 1:0.1-1. The hair loss prevention serum is specially designed for patients with androgenetic alopecia, and is mainly effective in preventing hair loss and improving hair quality. The core components are psoralen and dianinopyrimidine oxide, supplemented by South American amaranth root extract, glycerol, and cocoyl glutamic acid, and the serum has the characteristics of strong hair loss prevention and no irritation.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of biotechnology. More particularly, it relates to a hair loss prevention serum and a preparation method thereof. BACKGROUND

[0002] Hair loss is a common skin disease and frequently-occurring disease with abnormal excessive hair loss and sparse hair as the main clinical manifestations. Androgenetic alopecia (AGA) accounts for the highest proportion, reaching 70%. [1] .

[0003] 2,4-diaminopyrimidine as the mother nucleus structure of this type of compound shows hair loss prevention effect in the field of daily chemical raw materials. This type of compound includes 2,4-diaminopyrimidine-3-oxide (amennex) and 6-pyrrolidinyl-2,4-diaminopyrimidine-3-oxide (capixyl). The structural formulas of the three are compared in Table 1.1. Among them, amennex can inhibit the expression of lysine hydroxylase mRNA, disrupt the production of mature collagen by fibroblasts, prevent the deposition of mature collagen, and reduce the contraction of hair follicles; capixyl is a new water-soluble daily chemical raw material, which is safer and has better water solubility than minoxidil, has no side effects, and has been approved by Europe, the United States, and Japan for personal washing and protection products. It has a similar hair loss prevention mechanism as minoxidil, can effectively promote the proliferation of hair papilla cells, stimulate hair follicle regeneration, expand blood vessels, increase nutrients and oxygen for hair roots, achieve the effect of nourishing hair follicles, and can speed up new hair growth by optimizing the growth cycle of hair follicles [2] .

[0004] Table 1.1

[0005]

[0006] Patent application WO2007 / 101357A2 discloses that when pyrrolidinyl diaminopyrimidine oxide is combined with diaminopyrimidine oxide, it efficiently promotes hair growth, which is much stronger than the effect of using only diaminopyrimidine oxide or minoxidil; patent CN109528725B discloses a nano composition containing diaminopyrimidine oxide and pyrrolidinyl diaminopyrimidine oxide. The new hair growth promoter diaminopyrimidine oxide, pyrrolidinyl diaminopyrimidine oxide, and other active agents are co-encapsulated in the same nano composition through shear emulsification, high-pressure homogenization, etc. The nano composition targets five different targets of androgenetic alopecia, namely, inhibiting 5α-reductase activity, antagonizing androgen receptor expression, anti-inflammatory and antibacterial, improving scalp microcirculation, and antioxidant effect of hair follicles, which has a compound synergistic effect. Therefore, how to improve the hair loss prevention effect of a single component and develop more synergistic components is still the focus of current research and development.

[0007] [1] WANG T L, ZHOU C, SHEN Y W, et al. Prevalence of androgeneticalopecia in China: a community-based study in six cities[J]. Br J Dermatol, 2010, 162(4): 843-847.

[0008] [2] WANG Z P. Research on the anti-hair loss and hair growth nanoliposome[D]. Wuhan: Huazhong University of Science and Technology, 2020. SUMMARY

[0009] The present application is dedicated to the research of anti-hair loss products, and it is accidentally found that, in the past research, bakuchiol does not show obvious anti-hair loss effect, but when it is used with 2,4-diaminopyrimidine oxide, the anti-hair loss effect is obviously better than that of single use, and this property of bakuchiol is only embodied in the compounds with 2,4-diaminopyrimidine as the mother nucleus structure, so the present application is made.

[0010] The purpose of the present application is to provide an anti-hair loss serum.

[0011] Another purpose of the present application is to provide a preparation method of the anti-hair loss serum.

[0012] The above purposes of the present application are achieved by the following technical scheme: an anti-hair loss serum, comprising the following components in mass fraction: 1-3% of an anti-hair loss composition, 2-5% of a humectant, 1-5% of a solubilizer, 0.1-1% of a thickening agent, 1-3% of a hair conditioner, 1-3% of a soothing agent, 0.1-1% of a pH regulator, and the rest of a solvent; the anti-hair loss composition is composed of bakuchiol and 2,4-diaminopyrimidine oxide and its derivatives in a weight ratio of 1:0.1-1.

[0013] In one embodiment of the present application, the 2,4-diaminopyrimidine oxide and its derivatives are 2,4-diaminopyrimidine-3-oxide or 6-pyrrolidinyl-2,4-diaminopyrimidine-3-oxide.

[0014] In addition, the enhancing effect of bakuchiol is not observed on other substances with anti-hair loss effect listed in the embodiments of the present application, and the currently found substance is finasteride, and other substances are to be checked.

[0015] In one embodiment of the present application, the diaminopyrimidine oxide and its derivatives is 6-pyrrolidinyl-2,4-diaminopyrimidine-3-oxide. Among the three diaminopyrimidine oxide and its derivatives, the combination of bakuchiol and 6-pyrrolidinyl-2,4-diaminopyrimidine-3-oxide is observed to have the best anti-hair loss effect, and the combination of the two in the male hormone hair loss mouse model has the best effect, which is much greater than the sum of the two (1+1>2), and has a synergistic effect; the second is the combination of 6-(1-piperidinyl)-2,4-diaminopyrimidine-3-oxide.

[0016] In one embodiment of the present application, the weight ratio of bakuchiol and 6-pyrrolidinyl-2,4-diaminopyrimidine-3-oxide is any value between 1:0.1~1, such as 1:0.1, 1:0.15, 1:0.2, 1:0.25, 1:0.3, 1:0.35, 1:0.4, 1:0.45, 1:0.5, 1:0.55, 1:0.6, 1:0.65, 1:0.7, 1:0.75, 1:0.8, 1:0.85, 1:0.9, 1:0.95, 1:1. In the combination of bakuchiol and 6-pyrrolidinyl-2,4-diaminopyrimidine-3-oxide, not all ratios can produce satisfactory results. Experiments have confirmed that within the ratio of 1:0.1~1, the combination of the two shows additive or synergistic effect, and when the ratio is 1:0.5, the effect is optimal.

[0017] In one embodiment of the present application, the humectant is one or a mixture of two or more of glycerin, arginine and sodium hyaluronate; the solubilizing agent is one or a mixture of PEG-40 hydrogenated castor oil and PPG-1-PEG-9 lauryl glycol ether.

[0018] In one embodiment of the present application, the thickening agent is acrylate / beheneth-25 methacrylate copolymer; the pH adjuster is one or a mixture of aminomethyl propanol, sodium citrate, disodium hydrogen phosphate and citric acid; the solvent is one or a mixture of water, ethanol and denatured ethanol.

[0019] In one embodiment of the present application, the hair conditioner is one or a mixture of cocoyl glutamic acid, pyridoxine hydrochloride, methionine and hydrolyzed wheat protein; the soothing agent is one or a mixture of Amaranthus caudatus root extract, rhamnose and Salvia officinalis oil.

[0020] In one embodiment of the present application, the anti-hair loss composition comprises 1-3% of the anti-hair loss composition, 2-5% of glycerin, 1-5% of PEG-40 hydrogenated castor oil, 0.1-1% of acrylates / beheneth-25 methacrylate copolymer, 1-3% of cocoyl glutamate, 1-3% of Acalypha wilkesiana root extract, 0.1-1% of sodium citrate, 3-5% of ethanol, and the balance of water; the anti-hair loss composition is composed of psoralen and 6-pyrrolidinyl-2,4-diaminopyrimidine-3-oxide in a weight ratio of 1:0.1-1.

[0021] The glycerin in the formula is responsible for moisturizing, providing essential moisture to the hair and scalp; the Acalypha wilkesiana root extract has a soothing effect, which can reduce the irritation caused by other ingredients and relieve scalp discomfort; the cocoyl glutamate can condition the hair; the PEG-40 hydrogenated castor oil and ethanol can improve the dispersibility of psoralen in the water-based essence.

[0022] In one embodiment of the present application, the diaminopyrimidine oxide and its derivatives are 6-pyrrolidinyl-2,4-diaminopyrimidine-3-oxide. The reason for the preference is that, in the tests, it was found that, among the listed diaminopyrimidine oxides and their derivatives, the combination of psoralen and 6-pyrrolidinyl-2,4-diaminopyrimidine-3-oxide and 6-(1-piperidinyl)-2,4-pyrimidine diamine-3-oxide has the best anti-hair loss effect, and the combination has the best effect in a male hormone hair loss mouse model, and is much greater than the sum of the single use (1+1>2), producing a synergistic effect; and among the two, the combination with 6-pyrrolidinyl-2,4-diaminopyrimidine-3-oxide has the best effect, and has higher water solubility and lower irritation than 6-(1-piperidinyl)-2,4-pyrimidine diamine-3-oxide.

[0023] And, in these combinations, a significant improvement in hair quality was observed, manifested as a significant increase in hair diameter and an increase in scale length (see Fig. 3~4 ), and this effect was weaker or even not observed in single use.

[0024] The present application also provides a preparation method of the anti-hair loss essence.

[0025] Step S1, mix psoralen, PEG-40 hydrogenated castor oil, and ethanol, and stir until uniform to form a uniform premix;

[0026] Step S2, disperse the acrylic ester / benzyl alcohol polyether-25 methacrylate copolymer, glycerol, 6-pyrrolidinyl-2,4-diamino pyrimidine-3-oxide, cocoyl glutamic acid and amaranthus dubius root extract in deionized water, stir and heat to 80-85 DEG C, and keep warm for standby, to obtain the water phase;

[0027] Step S3, slowly add the premix of step S1 into the water phase under the condition of continuous stirring at 80-85 DEG C, to obtain a mixed solution, homogenize, cool to 40-45 DEG C, then add sodium citrate to adjust the pH to 5.0-6.5, stir, discharge, and obtain the anti-hair loss serum.

[0028] In the preparation process, since the water solubility of psoralen is poor, PEG-40 hydrogenated castor oil is used as a solubilizing agent, supplemented with ethanol, to significantly improve the dispersity and stability of psoralen in the water-based serum through solubilizing agent technology, so as to ensure that psoralen is uniformly dispersed in the water-based serum and plays the best effect.

[0029] The present application has the following beneficial effects:

[0030] The anti-hair loss serum is specially designed for androgenic alopecia patients, and mainly has the effects of strong anti-hair loss and improving hair quality. The core components are psoralen and combitol, supplemented with amaranthus dubius root extract, glycerol and cocoyl glutamic acid, and the like, and the serum has the characteristics of strong anti-hair loss and low irritation. BRIEF DESCRIPTION OF DRAWINGS

[0031] Fig. 1 Score and compare the hair growth of each group of mice;

[0032] Fig. 2 The hair coverage rate of each group of mice on the 28th day;

[0033] Compared with the blank control group, * P<0.01; compared with the model group, # P<0.05, ## P<0.01, ### P<0.001; compared with the Kopexil group, a P<0.05; compared with the minoxidil group, b P<0.05; compared with the combitol group, c P<0.01.

[0034] Fig. 3 Compare the hair diameter of each group of mice on the 28th day;

[0035] Fig. 4 Compare the hair scale length of each group of mice on the 28th day;

[0036] Among them, Fig. 3 andFig. 4 Compared with the model group, # P<0.05, ## P<0.01. DETAILED DESCRIPTION

[0037] The application will be further described below in connection with specific examples, but the examples do not limit the application in any form. Unless otherwise specified, the reagents, methods and equipment used in the application are conventional reagents, methods and equipment in the technical field.

[0038] Unless otherwise specified, the reagents and materials used in the following examples are commercially available.

[0039] Test Example 1, efficacy test

[0040] 1 Experimental section

[0041] 1.1 Experimental materials: 2,4-diaminopyrimidine-3-oxide (Kopexil, purchased from Wuhan Belka Biomedicine Co., Ltd.), 6-(1-piperidinyl)-2,4-pyrimidinediamine-3-oxide (minoxidil, purchased from Wuhan Belka Biomedicine Co., Ltd.), 6-pyrrolidinyl-2,4-diaminopyrimidine-3-oxide (Cobenil, purchased from Hubei Guangao Biotechnology Co., Ltd.); Bps (purchased from SYTHEON).

[0042] 1.2 Experimental method

[0043] 1.2.1 Animal grouping and coating method: 110 male C57BL / 6 mice were selected, and after 1 week of environmental adaptation, they were numbered according to their body weight and then randomly divided into 11 groups according to the body weight stratified block design method: blank control group, alopecia model group, 2wt% Bps group, 1wt% Kopexil group, 1wt% minoxidil group, 1wt% Cobenil group, 1wt% finasteride group, 2wt% Bps+1wt% Kopexil group, 2wt% Bps+1wt% minoxidil group, 2wt% Bps+1wt% Cobenil group and 2wt% Bps+1wt% finasteride group, 10 mice in each group.

[0044] The rosin and paraffin were mixed at a ratio of 1:1 and heated to melt, and then cooled to an appropriate temperature. The mixture was uniformly coated on the back of the mice with an area size of about 4 cm x 4 cm. After cooling and solidification, the mixture was removed. The mouse hair follicle growth entered the growth period, and the hair follicle and the spontaneously formed growth period hair follicle had no difference in morphology, and the skin was pink. The next day after the hair was removed, the mice were anesthetized, and the blank group was coated with 0.1 mL·cm -2 50% ethanol solution was applied once a day for 28 consecutive days; the mice in the other groups were coated with 1 mg·mL -1Testosterone solution, once a day, for 28 consecutive days. After 30 minutes of modeling every day, the blank group and the model group mice were coated with the base solution on the depilation area with a cotton swab, and the rest of the drug groups were coated with the corresponding drug solution, and the volume of the drug was 0.2 mL per mouse.

[0045] 1.2.2 Preparation of drug solution: ① Base solution: weigh 95% ethanol, propylene glycol and pure water according to the mass ratio of 3:6:2, mix thoroughly, and get the base solution; ② Psoralen group, Kopexil group, minoxidil group, combivent group and finasteride group: weigh the corresponding weight of the drug, add it to the base solution and dissolve uniformly to get the drug solution; ③ Combination group: weigh the corresponding weight of the drug and dissolve it in the base solution to get the drug solution of 2wt% psoralen + 1wt% Kopexil, 2wt% psoralen + 1wt% minoxidil, 2wt% psoralen + 1wt% combivent and 2wt% psoralen + 1wt% finasteride.

[0046] 1.2.3 Hair growth rate measurement: observe the color change and hair growth of the mouse skin, take photos after anesthesia on the 0th day, 7th day, 14th day, 21st day and 28th day, refer to the scoring standard and combine the actual situation to score the hair growth of each mouse according to the following table 1.

[0047] Table 1 Hair growth condition scoring standard

[0048]

[0049] According to the above scoring standard, three people score the hair growth of the mouse respectively, and the average value is taken as the final score. On the 28th day, the hair coverage area and the depilation area of the mouse are measured by software Image J, and the following formula is used for calculation.

[0050] Hair coverage rate (%) = hair coverage area / depilation area × 100%

[0051] 1.2.3 Hair length measurement: on the 29th day, the mice were sacrificed, and the newly grown hair in the depilation area was pulled out with tweezers (the number of hair > 20), and the longest 20 hairs were selected and measured with a vernier caliper, and the average length of the newly grown hair of the mouse was recorded.

[0052] 1.2.4 Hair quality measurement: randomly select the newly grown hair in the depilation area, take SEM photos, measure the middle end diameter of the hair in the picture with Image J to evaluate the thickness of the hair, measure the scale length of the middle end of the hair to evaluate the size of the scale, and comprehensively evaluate the quality of the newly grown hair of the mouse.

[0053] 1.2.5 HE staining to detect the number of skin hair follicles: After the mice were sacrificed, the depilated skin was cut off and placed in 4% paraformaldehyde tissue fixative for at least 24 h. After dehydration, paraffin embedding, sectioning, dehydration, hematoxylin-eosin staining, and mounting, the results were observed and recorded under an optical microscope (x100). The counting function of Image J was used to count the number of hair follicles in the HE staining pictures of each group, and 6 fields were taken from each group, and then the average value was calculated.

[0054] 2 Results and discussion

[0055] 2.1 Hair growth rate

[0056] The hair growth scores of the depilated areas of the mice in each group are shown in Table Fig. 1 , and the hair coverage is shown in Table Fig. 2 . Analysis shows that compared with the blank group, the hair growth of the model group became significantly slower, and the difference in growth rate became gradually obvious on day 14. Compared with the model group, the hair growth rate of the Kopexil group, the minoxidil group, the clobetasol group, and the finasteride group was significantly faster. In the combination group, except for the combination of bakuchiol + finasteride, the hair growth rate of the mice in the other groups was higher than that when used alone, and the difference was significant. This indicates that bakuchiol can enhance the hair growth effect of Kopexil, minoxidil, and clobetasol.

[0057] 2.2 Hair length and hair follicle number

[0058] As shown in the table below, the hair length and hair follicle number of the mice in each group of the model group were significantly reduced compared with the blank control group (P<0.01); compared with the model group, the hair length and hair follicle number of the bakuchiol group increased, but there was no significant difference, while the hair length and hair follicle number of the Kopexil, minoxidil, clobetasol, and finasteride groups were significantly or extremely significantly increased compared with the model group; in the combination group, except for the finasteride group, the hair length and hair follicle number of the mice in the other groups were extremely significantly higher than those in the single use group, and the difference was significant, especially in the bakuchiol + clobetasol group and the bakuchiol + minoxidil group, the combined effect was much greater than the sum of the single use (1+1>2), producing a synergistic effect.

[0059] Table 2 Hair length and hair follicle number

[0060]

[0061] Compared with the blank group, ** P<0.01; compared with the model group, # P<0.05, ## P<0.01; compared with the Kopexil group, a P<0.05; compared with the minoxidil group,b P<0.01; compared with the comparable group, c P<0.01.

[0062] 2.3 Hair quality

[0063] Comparison of hair diameter in the depilation area of mice in each group Fig. 3 Comparison of hair scale length Fig. 4 The hair diameter and hair scale length of the model group, Kopexil group, minoxidil group, comparable group, and finasteride group had no significant difference compared with the blank group, but the hair diameter of the mice in the psoralen and psoralen combination groups increased significantly, and the hair scale length increased, which indicated that psoralen not only enhanced the effect of preventing the hair growth promoting effect of diaminopyrimidine oxide, but also improved the quality of the new hair.

[0064] Test Example Two, Effect Verification of Psoralen and Comparable Promoting Hair Growth in Different Quality Ratios

[0065] It was verified by Test One that the combination of psoralen and comparable had the best anti-hair loss effect. Further, Test One was used to investigate the effect of different ratios of psoralen and comparable on the hair growth rate and hair follicle number of the male hormone alopecia mouse model, and the results are shown in Table 3 below.

[0066] Table 3 Test Results

[0067]

[0068] Compared with the blank group, ** P<0.01; compared with the model group, # P<0.05, ## P<0.01; compared with the comparable group, ^ P<0.05, ^^ P<0.01.

[0069] Example One, Anti-hair loss double-layer essence

[0070] Ingredients Amount (mass fraction) Glycerin 3% Bakuchiol 2% Cobaea 1% PEG-40 Hydrogenated Castor Oil 5% Acrylates / Beheneth-25 Methacrylate Copolymer 0.5% Cocoyl Glutamate 1.5% Acmella Oleracea Root Extract 2% Sodium Citrate Adjust pH to 5.5 Ethanol 3% Water Balance

[0071] Preparation process:

[0072] Step S1, mix psoralen, PEG-40 hydrogenated castor oil, and ethanol, stir evenly, and form a uniform premix;

[0073] Step S2, disperse acrylate / behenyl alcohol polyether-25 methacrylate copolymer, glycerol, comparable, cocoyl glutamic acid, and amaranthus dubius root extract in deionized water, stir and heat to 85°C, and keep warm for standby, to obtain an aqueous phase;

[0074] Step S3, the premix of step S1 is slowly added into the water phase under the condition of continuous stirring at 85℃ to obtain a mixture, homogenized, cooled to 45℃, then sodium citrate is added to adjust the pH to 5.5, stirred, discharged, to obtain the anti-hair loss serum.

[0075] Example two, anti-hair loss double-layer serum

[0076] Ingredients Amount (mass fraction) Glycerin 3% Bakuchiol 2% Kopexil 1% PEG-40 Hydrogenated Castor Oil 5% Acrylates / Beheneth-25 Methacrylate Copolymer 0.5% Cocoyl Glutamate 1.5% Acmella Oleracea Root Extract 2% Sodium Citrate Adjust pH to 5.5 Ethanol 3% Water Balance

[0077] Preparation process reference example one.

[0078] House rabbit skin irritation test

[0079] The skin irritation method in the 2015 edition of the Cosmetic Safety Technology Standard is referred to, 12 rabbits are selected for the test, half male and half female, weighing 2.0±0.5 kg, randomly divided into three groups, each group of 4, named as example one~three groups. 24h before the test, the back of each group of rabbits is shaved with depilatory cream, and the shaved area is 6cm×6cm. Using the self-control method, the right side of each rabbit is applied with the corresponding anti-hair loss serum, once a day, continuously for 7 days, and the rabbit's depilation area is observed before each administration for edema and erythema. Observe the rabbit's skin irritation reaction at 1, 6, 12, 24, 48h after stopping administration, and score according to the scoring standard. According to the formula: average value of irritation reaction=(redness score+edema score) / number of test animals, calculate the average value of irritation score of each rabbit within the observation period, judge the skin irritation intensity, and the results are shown in Table 4.

[0080] Table 4 Results of rabbit skin irritation test (total score)

[0081] Group 1 6 12 24 48 Example 1 0 0 0 0 0 Example 2 0 0 0 0 0

[0082] From the above table, the skin of each group of rabbits applied with the anti-hair loss serum of the present application example one and two has no irritation such as itching, bleeding points, etc., and there is no erythema and edema after application for 1, 6, 12, 24, 48h, and the average value of each time point score is 0, indicating no skin irritation.

[0083] Mouse skin allergy test

[0084] Select Kunming mice 50, half male and female, body weight in 20~25 g, divided into positive control group (1% of 2,4-dinitrochlorobenzene, each dosage 0.2 ml), negative control group (pure water), sample group (example one~three groups). Each group of mice using depilatory cream in the back of 4cm×4cm size area, observed for 24h, to ensure that the mouse skin is not damaged, sensitization dosing: 1st day dosing 1 time, then again on the seventh day and the fourteenth day dosing sensitization, drug smearing in the right side, need to use plastic wrap to keep the skin moist, then use gauze in the protection of covering, finally fixed with adhesive tape and bandage, fixed 6h, then wash with warm water. Challenge dosing: on the fourteenth day of the last sensitization in mice left side sensitization, then respectively in 24h, 48h, 72h observation of erythema, edema and other symptoms scoring. According to table 5 standard to skin reaction score, and calculate the incidence of allergic reaction, according to table 6 to judge the intensity of allergic reaction. Sensitization rate = redness or edema animal number ÷ total number of test animals, the results are shown in table 7.

[0085] Table 5 skin irritation reaction score

[0086]

[0087] Table 6 sensitization intensity

[0088] Sensitization rate (%) Classification Sensitization intensity 0~8 Ⅰ Weak sensitivity 9~28 Ⅱ Mild sensitization 29~64 Ⅲ Moderate sensitization 65~80 Ⅳ Strong sensitization 81~100 Ⅴ Extreme sensitization

[0089] Table 7 mouse skin allergy test results

[0090]

[0091] From the above table, the positive control group mice in the last 24, 48 and 72h after sensitization, the sensitization rate was 70%, 40% and 20%, respectively, there is an allergic phenomenon to the skin, the blank group and the present application example one, two groups did not cause allergic phenomenon, prompting its no sensitization to the skin.

[0092] The above examples are the preferred embodiments of the present application, but the embodiments of the present application are not limited by the above examples, any change, modification, substitution, combination, simplification made without departing from the spirit and principles of the present application, should be equivalent to the replacement mode, all included in the protection scope of the present application.

Claims

1. A hair loss prevention serum, characterized by, A composition comprising the following components by mass fraction: 1-3% of a hair loss prevention composition, 2-5% of glycerin, 1-5% of PEG-40 hydrogenated castor oil, 0.1-1% of acrylate / beheneth-25 methacrylate copolymer, 1-3% of cocoyl glutamate, 1-3% of amaranthus dubius root extract, 0.1-1% of sodium citrate, 3-5% of ethanol, and the balance of water; wherein the hair loss prevention composition is composed of psoralen and 6-pyrrolidinyl-2,4-diaminopyrimidine-3-oxide in a weight ratio of 1:0.1-1.

2. The hair loss prevention serum according to claim 1, characterized in that, The hair loss prevention composition is composed of psoralen and 6-pyrrolidinyl-2,4-diaminopyrimidine-3-oxide in a weight ratio of 1:0.

5.

3. The method of claim 1 or 2, wherein the anti-hair loss serum is prepared by adding the following components to water and then stirring the mixture: Prepared by the following method: ​ Step S1, mix psoralen, PEG-40 hydrogenated castor oil and ethanol, stir until uniform, to form a uniform premix; Step S2, disperse acrylate / beheneth-25 methacrylate copolymer, glycerin, 6-pyrrolidinyl-2,4-diaminopyrimidine-3-oxide, cocoyl glutamate and amaranthus dubius root extract in deionized water, stir and heat to 80-85°C, keep warm for standby, to obtain an aqueous phase; Step S3, slowly add the premix of step S1 to the aqueous phase at 80-85°C under continuous stirring, to obtain a mixture, homogenize, cool to 40-45°C, then add sodium citrate to adjust the pH to 5.0-6.5, stir, discharge, to obtain a hair loss prevention serum.

Images