Application of paris polyphylla aqueous extract in treatment of psoriasis
By using the Seven Leaf Flower Water Extract to treat psoriasis, the problems of adverse reactions and high cost in existing treatment methods are solved, and effective treatment of psoriasis is achieved, and high economic and safety are achieved.
Patent Information
- Application Number
- CN202510384597.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-28
- Publication Date
- 2025-06-13
AI Technical Summary
In the prior art, treatment methods for psoriasis, such as topical drugs, systemic therapy and physical therapy, have problems such as inducing adverse reactions such as potential infections and are expensive.
The water extract of seven leaves and one branch flower was used as the drug for treating psoriasis. The water extract was prepared through specific extraction methods and verified the therapeutic effect of psoriatic mice.
The water extract of seven leaves and one branch flower can significantly reduce the infiltration of subcutaneous inflammatory cells in psoriatic mice, inhibit vascular hyperplasia, improve inflammatory factors, and have high economic and safety. Their efficacy is similar to that of chemical synthetic drugs.
Smart Images
Figure CN120131820A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of biomedicine, and particularly relates to an application of a water extract of Parsley septemlobus for treating psoriasis. Background Art
[0002] Psoriasis is a chronic, recurrent, inflammatory, systemic skin disease mediated by the immune system. The main clinical manifestations are erythema of varying sizes on the body covered with a large amount of white scales. The course of the disease is prolonged, prone to relapse, and difficult to cure. In addition, psoriasis patients are often accompanied by comorbidities such as cardiovascular disease, metabolic syndrome, cancer, and depression. These comorbidities synergistically increase the physical and psychological burden of patients and also bring huge social burdens. According to epidemiological surveys, there are more than 60 million psoriasis patients worldwide. At present, the main clinical treatment methods for psoriasis are topical medications, systemic treatments, and physical therapy. However, it has adverse reactions such as inducing potential infections and is expensive, so the indications must be strictly controlled when used. Therefore, it is particularly important to urgently find economical, safe, and effective drugs to treat psoriasis. Summary of the invention
[0003] In view of this, the present invention provides an application of a water extract of Parsley septemlobus for treating psoriasis, so as to solve the technical problems in the prior art that topical drugs, systemic treatments and physical treatments have adverse reactions such as inducing potential infections and are expensive.
[0004] To achieve the above objectives, this application adopts the following scheme: Application of a water extract of Parsley septififlora to treat psoriasis.
[0005] Preferably, the water extract of Parsley is prepared by the following method: S10. Weigh and slice the medicinal material of Psoralea corylifolia; S20. Add distilled water to the slices and soak them at room temperature for 16 to 20 hours, wherein the mass ratio of the slices to the distilled water is 780 g: (7.8 to 8.2) L; S30. After soaking, the first decoction is performed; S40. After the first decoction is completed, filter, take the residue and add water, and perform a second decoction; S50. After the second decoction is completed, the residue is filtered and discarded, the two filtrates are combined and filtered with gauze, and the filtrate is evaporated and concentrated to obtain the water extract of Polygonum multiflorum.
[0006] Preferably, distilled water is added to the slices and soaked at room temperature for 18 hours, and the mass ratio of the slices to the distilled water is 780g:8L.
[0007] Preferably, the first decoction is the same as the second decoction, both of which are to boil strongly first and then decoct slowly for 1 h.
[0008] Preferably, the dosage of the water extract of Paris polyphylla is 0.3 g / kg to 2.4 g / kg.
[0009] Preferably, the dosage of the water extract of Paris polyphylla is 2.4 g / kg.
[0010] In the above application of the water extract of Paris polyphylla in treating psoriasis, after treating psoriasis mice with the water extract of Paris polyphylla for a period of time, it can significantly reduce the infiltration of inflammatory cells under the skin of psoriasis mice, indicating that the water extract of Paris polyphylla can reduce the inflammatory reaction of psoriasis patients; it can inhibit the angiogenesis of psoriasis mice, thus helping to improve the vascular dilation performance of psoriasis patients; it can improve the inflammatory factors in psoriasis mice, indicating that the water extract of Paris polyphylla can treat psoriasis by regulating the immune inflammatory reaction; it can also make the body weight of psoriasis mice recover, indicating that the water extract of Paris polyphylla can improve the interference of the whole body metabolism of psoriasis patients, improve chronic inflammation, and also has the effect of inhibiting epidermal thickening. Therefore, the water extract of Paris polyphylla helps to improve psoriasis, and the curative effect is the same as that of methotrexate. It can be seen that it has a rapid onset and good curative effect.
[0011] Compared with the chemically synthesized drugs for treating psoriasis, the raw material source of the water extract of Paris polyphylla provided by the present invention is more extensive and easier to obtain. At the same time, as a renewable resource, Paris polyphylla has relatively low planting and collection costs, which helps to reduce the production cost of drugs. And the water extraction method, as a traditional extraction method, has mature technology and low equipment requirements, further reducing the production cost. In addition, compared with the chemically synthesized drugs for treating psoriasis, the water extract of Paris polyphylla has less toxicity and fewer side effects on the human body. While exerting the drug effect, the water extract of Paris polyphylla has less irritation and damage to the human body. And long-term use of chemically synthesized drugs is likely to cause microorganisms to develop drug resistance, thus reducing the curative effect of drugs. However, the natural components in the water extract of Paris polyphylla have diversified antibacterial mechanisms, which can not only directly kill or inhibit the growth of bacteria, but also improve the antibacterial ability by regulating the human immune system and other ways, thus reducing the generation of drug resistance. Therefore, the water extract of Paris polyphylla provided by the present invention also has high economy and safety in treating psoriasis. Description of the Drawings
[0012] Figure 1 It is a representative photo of the back skin of mice in each group on the 7th day in the present invention.
[0013] Figure 2 It is a graph of the total PASI score results of mice in the present invention.
[0014] Figure 3 These are the histopathological observation diagrams of the skin tissues of mice in each group in the present invention.
[0015] Figure 4 This is the curve graph of the body weight changes of mice in each group in the present invention. Detailed implementation manners
[0016] To make the objectives and technical solutions of the present invention clearer and more understandable, the content of the present invention will be further described in detail through experiments. However, it should not be understood that the scope of the above-mentioned subject matter of the present invention is limited to the following experiments only. Any corresponding replacement or modification made according to the common general knowledge and customary means in the art without departing from the above-mentioned technical premise of the present invention is included in the present invention.
[0017] In a specific embodiment, the present invention provides an application of the water extract of Paris polyphylla in the treatment of psoriasis. It is proved by the following experimental examples that after treating psoriasis mice with the water extract of Paris polyphylla for a period of time, it can significantly reduce the infiltration of inflammatory cells under the skin of psoriasis mice, indicating that the water extract of Paris polyphylla can alleviate the inflammatory reaction of psoriasis patients; it can inhibit the angiogenesis of psoriasis mice, thus helping to improve the vascular dilation performance of psoriasis patients; it can improve the inflammatory factors in the body of psoriasis mice, indicating that the water extract of Paris polyphylla can treat psoriasis by regulating the immune inflammatory reaction; it can also make the body weight of psoriasis mice recover, indicating that the water extract of Paris polyphylla can improve the interference of the whole body metabolism of psoriasis patients, improve chronic inflammation, and also has the effect of inhibiting epidermal thickening. Therefore, the water extract of Paris polyphylla helps to improve psoriasis, and its curative effect is the same as that of methotrexate. It can be seen that it has a fast onset and good curative effect.
[0018] Compared with the chemically synthesized drugs for treating psoriasis provided by the present invention, the raw material source of the water extract is more extensive and easier to obtain. At the same time, as a renewable resource, Paris polyphylla has relatively low planting and collection costs, which helps to reduce the production cost of drugs. Moreover, the water extraction method, as a traditional extraction method, has mature technology and low equipment requirements, further reducing the production cost. In addition, compared with the chemically synthesized drugs for treating psoriasis, the water extract of Paris polyphylla has less toxicity and fewer side effects on the human body. When the water extract of Paris polyphylla exerts its medicinal effect, it has less irritation and damage to the human body. And long-term use of chemically synthesized drugs is likely to cause microorganisms to develop drug resistance, thereby reducing the curative effect of drugs. However, the natural components in the water extract of Paris polyphylla have diversified antibacterial mechanisms. They can not only directly kill or inhibit the growth of bacteria, but also improve the antibacterial ability by regulating the human immune system and other ways, thus reducing the generation of drug resistance. Therefore, the water extract of Paris polyphylla provided by the present invention also has high economy and safety when treating psoriasis.
[0019] The preparation method of the Paris polyphylla aqueous extract in this embodiment is as follows: Step 1: Select an appropriate amount of Paris polyphylla medicinal materials and cut them into slices for subsequent soaking and decocting.
[0020] Step 2: Put the cut Paris polyphylla medicinal material slices into a container, add distilled water according to the ratio of 780 g of medicinal material slices corresponding to (7.8 to 8.2) L of distilled water, soak at room temperature for 16 h to 20 h to improve the efficiency of subsequent decocting. Preferably, add distilled water according to the ratio of 780 g of medicinal material slices corresponding to 8 L of distilled water and soak at room temperature for 18 h; Step 3: Conduct the first decoction on the soaked medicinal material slices together with the soaking solution. During the first decoction process, the heat and time should be controlled to fully extract the active ingredients in the medicinal materials; Step 4: After the first decoction is completed, use a filtering device to separate the decoction liquid from the medicinal material residues. Then, add an appropriate amount of water to the filter residues for the second decoction to further extract the remaining active ingredients in the medicinal materials. Preferably, the first decoction is the same as the second decoction, both starting with high heat to boil and then slow heat decocting for 1 h; Step 5: After the second decoction is completed, filter again, discard the filter residues, combine the filtrates obtained from the two decoctions, and conduct fine filtration using a gauze to remove impurities. Finally, conduct water-bath evaporation and concentration on the filtrate until the required Paris polyphylla aqueous extract is obtained.
[0021] The prepared Paris polyphylla aqueous extract can relieve the skin symptoms of psoriasis patients, such as erythema, scales, itching, etc. Through anti-inflammatory and immunomodulatory effects, the Paris polyphylla aqueous extract can promote the recovery process of psoriasis patients and reduce the risk of recurrence.
[0022] Furthermore, the dosage of the Paris polyphylla aqueous extract is 0.3 g / kg to 2.4 g / kg, preferably 2.4 g / kg.
[0023] The following through specific experimental examples further illustrate the technical solutions and technical effects of the present invention. It should be noted that the inventors have carried out relevant pharmacodynamic experimental studies to prove the efficacy of the Paris polyphylla aqueous extract in the treatment of psoriasis in the present invention. It should be noted that the following experimental studies are all carried out on the basis of proving the drug safety, and the administered doses in the experimental studies are all within the safe dose range. The drugs selected in the following pharmacodynamic tests are the drugs obtained from the representative formulations of the present invention and their preparation methods; the inventors have also carried out pharmacodynamic experiments on other drugs, and the experimental results show the same or similar effects, but due to space limitations, they are not listed one by one here.
[0024] 1. Drug materials Methotrexate (from the market); 5% IMQ ointment (commercially available); Water extract of Paris polyphylla Smith (prepared by the laboratory of Ningxia Medical University).
[0025] 2. Related technical solutions 2.1 Collection of experimental animals SPF-grade male BALB / c mice with a body weight of 18 g to 22 g were selected, provided by the Experimental Animal Center of Ningxia Medical University, and freely drank water and were fed with a fixed amount of mouse feed in an animal room with ventilation, dryness, a room temperature of 22°C to 25°C, and a humidity of 50% to 70%.
[0026] 2.2 Grouping of experimental animals Twenty-eight SPF-grade BALB / c mice that passed the quarantine were randomly divided into 4 groups, namely the normal group, the model group, the methotrexate group, and the water extract of Paris polyphylla Smith group.
[0027] 2.3 Model establishment The SPF-grade male BALB / c mice in the above-mentioned model group, methotrexate group, and water extract of Paris polyphylla Smith group were given an adaptive feeding for 2 d, and then a classical IMQ-induced psoriasis-like skin lesion model was established. One day before the experiment, the backs of the mice were subjected to relevant hair removal treatments. First, a pet hair clipper was used to basically remove the hair on the backs of the mice, and then Veet hair removal cream was used to thoroughly remove the remaining back fluff, obtaining a smooth back skin of the mice with an area of 2 * 3 cm. The treated mice were normally fed for 2 d to eliminate the possible damage to the back skin of the mice caused by the hair clipper and the hair removal cream; The mice in the normal group were smeared with 62.5 mg of white petrolatum on the back skin every day, and 0.2 mL of normal saline was given by gavage 1 h later, for 7 consecutive days; The mice in the model group were smeared with 62.5 mg of 5% (mass fraction) IMQ ointment (imiquimod) every day, and 0.2 mL of normal saline was given by gavage 1 h later, for 7 consecutive days; The mice in the methotrexate group were smeared with 62.5 mg of 5% (mass fraction) IMQ ointment every day, and 1 mg / kg of methotrexate was given by gavage 1 h later, for 7 consecutive days; The mice in the water extract of Paris polyphylla Smith group were smeared with 62.5 mg of 5% (mass fraction) IMQ ointment every day, and 2.4 g / kg of the water extract of Paris polyphylla Smith was given by gavage 1 h later, for 7 consecutive days; Before each administration to the mice in the above groups, photographs were taken for recording. After the last administration, as Figure 1 shown, the mice were decapitated and sacrificed.
[0028] 2.4 PASI scoring The characteristic changes of psoriasis such as erythema, skin lesion thickening, and scales on the backs of mice in each group were observed by direct observation, and the evaluation was carried out with reference to the internationally accepted method for evaluating the area and severity of psoriasis skin lesions (PASI). The evaluation results are as Figure 2 shown, and the specific judgment criteria are shown in Table 1: Table 1 PASI scoring table
[0029] It is Figure 1 and Figure 2 reflected that compared with the normal group, the mice in the model group showed obvious erythema, scales, and thickness, indicating that the modeling was successful; compared with the model group, the symptoms of skin erythema, scales, and thickness in the mice treated with the aqueous extract of Paris polyphylla Smith were significantly improved (see Figure 1 ), and the total PASI score was significantly reduced (see Figure 2 ), which was equal to the total score of the methotrexate group, indicating that it had a rapid onset and good efficacy. The results showed that the aqueous extract of Paris polyphylla Smith could effectively relieve the symptoms of psoriasis skin lesions in mice.
[0030] 2.5 H&E staining The pathological changes of the back skin of mice in each group were analyzed by H&E staining, and the results are as Figure 3 shown.
[0031] It is Figure 3 shown by the experimental results in
[0032] Figure 3 that after imiquimod was applied, compared with the normal group mice, the skin of the model group mice showed erythema, scales, and infiltration, producing psoriasis-like skin lesions. With the continuous action of the drug, the skin lesions of the model group became increasingly severe from the 2nd day to the 3rd day, the scales increased and thickened, and the skin infiltration was obvious. The comprehensive PASI score results of the model group were statistically significant on the 7th day (P<0.0001), indicating that the psoriasis mouse model was successfully established. After HE staining, the degree of lesions in the back skin tissue of mice was observed and compared under the microscope. Compared with the normal group, under the optical microscope, the epidermis of the skin lesions of the model group mice was significantly thickened, and obvious hyperkeratosis, parakeratosis, spongiosis, blood vessel dilation, and obvious congestion and other characteristic pathological changes of psoriasis were observed. The aqueous extract of Paris polyphylla Smith group could reduce the degree of epidermal thickening, reduce hyperkeratosis, parakeratosis, and acanthosis, indicating that the aqueous extract of Paris polyphylla Smith had a good therapeutic effect on psoriasis. The skin lesions of the methotrexate group mice were slightly reduced compared with those of the model group at the same stage. The skin of the methotrexate group mice was slightly reddened, with fewer scales and less infiltration and thickening, indicating that the aqueous extract of Paris polyphylla Smith had a protective effect on the psoriasis skin lesion changes caused by imiquimod.
[0032] The epidermal thickness of the mice in the Paris polyphylla Smith water extract group was significantly reduced, indicating that the Paris polyphylla Smith water extract may have the effect of inhibiting epidermal thickening, thus contributing to the improvement of the skin pathological manifestations of psoriasis; the infiltration of subcutaneous inflammatory cells in the mice of the Paris polyphylla Smith water extract group was significantly reduced, indicating that the Paris polyphylla Smith water extract may have an anti-inflammatory effect and can reduce the inflammatory response of psoriasis; the number of newly formed capillaries in the mice of the Paris polyphylla Smith water extract group was reduced, indicating that the Paris polyphylla Smith water extract may have the effect of inhibiting angiogenesis, thus contributing to the improvement of the vascular abnormalities in psoriasis.
[0033] 2.6 Body weight changes During the administration period, the mice in each group were weighed daily, and the results are as Figure 4 shown.
[0034] It can be seen through Figure 4 that the body weight of the mice in the normal group showed a gradually increasing trend, while the body weight of the mice in the model group showed a gradually decreasing trend. This may be due to the interference of psoriasis on the whole body metabolism of the mice and the consumption of energy by chronic inflammation. However, the body weights of the mice in the methotrexate group and the Paris polyphylla Smith water extract group recovered compared with the model group, indicating that the Paris polyphylla Smith water extract can improve the interference of psoriasis on the whole body metabolism of the mice and improve chronic inflammation.
[0035] 3. Experimental results The Paris polyphylla Smith water extract can significantly reduce the infiltration of subcutaneous inflammatory cells, indicating that it can reduce the inflammatory response of psoriasis; it can also inhibit angiogenesis, thus contributing to the improvement of the vascular dilation in psoriasis; and it can also improve inflammatory factors, treat psoriasis by regulating the immune inflammatory response, and also has the effect of inhibiting epidermal thickening and improving the interference of psoriasis on the whole body metabolism.
[0036] In summary, the Paris polyphylla Smith water extract helps to improve psoriasis.
[0037] As described above, it is only the specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art in the technical field disclosed by the present invention can easily think of various equivalent modifications or substitutions, and these modifications or substitutions should be covered within the protection scope of the present invention.
Claims
1. An application of a water extract of Parsley septemlobus for treating psoriasis.
2. The use of the water extract of Parsley chinensis for treating psoriasis according to claim 1, characterized in that: The water extract of Parsley septififolia is prepared by the following method: S10. Weigh and slice the medicinal material of Psoralea corylifolia; S20. Add distilled water to the slices and soak them at room temperature for 16 to 20 hours, wherein the mass ratio of the slices to the distilled water is 780 g: (7.8 to 8.2) L; S30. After soaking, the first decoction is performed; S40. After the first decoction is completed, filter, take the residue and add water, and perform a second decoction; S50. After the second decoction is completed, the residue is filtered and discarded, the two filtrates are combined and filtered with gauze, and the filtrate is evaporated and concentrated to obtain the water extract of Polygonum multiflorum.
3. The use of the water extract of Parsley chinensis for treating psoriasis according to claim 2, characterized in that: Distilled water was added to the slices and soaked for 18 h at room temperature, and the mass ratio of the slices to the distilled water was 780 g:8 L.
4. The use of the water extract of Parsley chinensis for treating psoriasis according to claim 2, characterized in that: The first decoction is the same as the second decoction, both of which are first boiled over high heat and then simmered over low heat for 1 hour.
5. The use of the water extract of Parsley chinensis for treating psoriasis according to claim 1, wherein the dosage of the water extract of Parsley chinensis is 0.3 g / kg to 2.4 g / kg.
6. The use of the water extract of Parsley septififlora for treating psoriasis according to claim 1, wherein the dosage of the water extract of Parsley septififlora is 2.4 g / kg.