Method for regulating and controlling multi-barrier function of small intestinal mucosa of early-stage weaned piglets by using Chinese

Abstract

The invention discloses a method for regulating and controlling a multi-barrier function of a small intestinal mucosa of an early weaned piglet by a Chinese herbal medicine, and relates to the technical field of animal husbandry, and the Chinese herbal medicine comprises the following Chinese herbal medicine raw materials: 10 parts of codonopsis pilosula, 10 parts of bighead atractylodes rhizome, 10 parts of poria cocos, 10 parts of honey-fried licorice root, 10 parts of Chinese yam, 10 parts of acanthopanax, 5 parts of coix seed, 5 parts of lotus seed, 5 parts of fructus amomi and 5 parts of malt. Removing metal impurities by adopting an X-ray foreign matter sorting machine, circularly washing by flowing pure water, and centrifugally dewatering after washing; the Chinese herbal medicine composition can effectively enhance the multi-barrier function of small intestinal mucosa of early weaned piglets, especially physical barriers and chemical barriers, reduce the permeability of intestinal tracts, improve the defense capacity of the intestinal tracts, remarkably reduce the diarrhea rate of the piglets and improve the health of the intestinal tracts by adjusting the immune response of the intestinal tracts and balancing the micro-ecology of the intestinal tracts, and has the effect of improving the immunity of the piglets. The method is simple and easy to implement, wide in Chinese herbal medicine raw material source, low in cost and suitable for large-scale breeding.

Description

Technical Field

[0001] The present invention relates to the technical field of animal husbandry, and particularly to a method for regulating the multi-barrier function of the small intestinal mucosa of early-weaned piglets with Chinese herbal medicines. Background Art

[0002] The health of piglets is greatly affected by intestinal health, because the intestines of piglets are an important place for digesting and absorbing nutrients, and play an extremely important role in maintaining the normal and healthy growth and development of piglets.

[0003] According to the patent titled: A Chinese herbal medicine composition for regulating the intestinal health of piglets, its preparation method and application (patent publication number: CN107050409A, patent publication date: August 18, 2017), it is composed of Atractylodes lancea 5 - 10 g, Atractylodes macrocephala 3 - 8 g, Dry ginger 7 - 12 g, Tangerine peel 3 - 5 g, Hawthorn fruit (processed) 2 - 5 g, Medicated leaven (processed) 3 - 5 g, Germinated barley (processed) 2 - 5 g. The present invention further discloses the application of the Chinese herbal medicine composition for regulating the intestinal health of piglets in improving the intestinal morphology and intestinal flora of piglets, and enhancing the production performance of piglets by promoting intestinal health. The results show that adding the Erzhu Sanxian powder to the piglet feed can improve the intestinal morphology and intestinal flora of piglets, and has a significant effect on promoting intestinal health.

[0004] Based on the above existing technologies, the current existing methods for regulating the multi-barrier function of the small intestinal mucosa of early-weaned piglets with Chinese herbal medicines still have the following problems. Early weaning is an important measure to improve the reproductive efficiency of sows and the growth rate of piglets in animal husbandry, but weaning stress often leads to damage to the small intestinal mucosal barrier function of piglets, and easily causes problems such as diarrhea. Although existing research has preliminarily explored the effects of Chinese herbal medicines on the growth performance and blood indexes of animals, there is still a lack of in-depth research on the systematic regulation mechanism of the multi-barrier function of the small intestinal mucosa. Therefore, the present invention provides a method for regulating the multi-barrier function of the small intestinal mucosa of early-weaned piglets with Chinese herbal medicines. Summary of the Invention

[0005] Aiming at the deficiencies of the existing technologies, the present invention provides a method for regulating the multi-barrier function of the small intestinal mucosa of early-weaned piglets with Chinese herbal medicines, and solves the following problems existing in the current existing methods for regulating the multi-barrier function of the small intestinal mucosa of early-weaned piglets with Chinese herbal medicines. Early weaning is an important measure to improve the reproductive efficiency of sows and the growth rate of piglets in animal husbandry, but weaning stress often leads to damage to the small intestinal mucosal barrier function of piglets, and easily causes problems such as diarrhea. Although existing research has preliminarily explored the effects of Chinese herbal medicines on the growth performance and blood indexes of animals, there is still a lack of in-depth research on the systematic regulation mechanism of the multi-barrier function of the small intestinal mucosa.

[0006] To achieve the above object, the present invention is realized through the following technical solutions: A method for regulating the multi-barrier function of the small intestinal mucosa of early-weaned piglets with Chinese herbal medicines, wherein the raw materials of Chinese herbal medicines include: 10 parts of Codonopsis pilosula, 10 parts of Atractylodes macrocephala, 10 parts of Poria cocos, 10 parts of roasted licorice root, 10 parts of Chinese yam, 10 parts of Eleutherococcus senticosus, 5 parts of Coix lacryma-jobi, 5 parts of lotus seeds, 5 parts of Amomum villosum, and 5 parts of malt. The regulation method is as follows:

[0007] A1. First, use an X-ray foreign object sorter to remove metal impurities, and rinse with circulating pure water. After rinsing, perform centrifugal dehydration.

[0008] A2. Second, perform segmented temperature-controlled drying on the dehydrated medicinal materials.

[0009] A3. Then, put the dried medicinal materials into an air-flow pulverizer for pulverization. After pulverization, irradiate with cobalt-60 γ rays and mix them together.

[0010] Preferably, in A1, the medicinal materials are rinsed 3 times with circulating pure water, and each rinsing time is 5 minutes.

[0011] Preferably, in A2, the temperatures for segmented temperature-controlled drying are 40°C for 2 hours, 50°C for 4 hours, and 60°C for 2 hours.

[0012] Preferably, in A3, the pulverized particle size ≤ 3 mm, the grading wheel speed is 2800 rpm, the pulverization pressure is 0.8 MPa, the finished product particle size D50 = 45 ± 5 μm, and the specific surface area ≥ 350 m 2 / kg.

[0013] Preferably, in A3, the dose rate of cobalt-60 γ ray irradiation is 1.5 kGy / h, and the cumulative dose is 8 kGy.

[0014] Preferably, the mixing in A3 adopts a gradient mixing process, which is divided into basic layer mixing, functional layer addition, synergistic layer integration, and overall mixing.

[0015] Preferably, in the basic layer mixing, Codonopsis pilosula, Atractylodes macrocephala, and Poria cocos are mixed, accounting for 30% of the total mass. In the functional layer addition, Eleutherococcus senticosus, roasted licorice root, and Chinese yam are mixed, accounting for 25%, and a 5% glycerol aqueous solution is sprayed synchronously. In the synergistic layer integration, Coix lacryma-jobi, lotus seeds, Amomum villosum, and malt are mixed, accounting for 15%. In the overall mixing, the three-layer materials are mixed and protected with nitrogen.

[0016] Preferably, 0.5% of nano-silica is added as a fluidizing agent during the overall mixing process.

[0017] The present invention provides a method for regulating the multi-barrier function of the small intestinal mucosa of early-weaned piglets with Chinese herbal medicines. Compared with the prior art, it has the following beneficial effects:

[0018] 1. A method for regulating the multi-barrier function of the small intestinal mucosa of early-weaned piglets. The Chinese herbal medicine composition of the present invention can effectively enhance the multi-barrier function of the small intestinal mucosa of early-weaned piglets, especially the physical barrier and chemical barrier, reduce intestinal permeability, improve intestinal defense ability, regulate intestinal immune response, balance intestinal microecology, significantly reduce the diarrhea rate of piglets, improve intestinal health, and the method is simple and easy to implement. The raw materials of Chinese herbal medicine are widely sourced and low in cost, suitable for large-scale farming.

[0019] 2. A method for regulating the multi-barrier function of the small intestinal mucosa of early-weaned piglets. Through a temperature gradient of low-temperature start, medium-temperature transition, and moderate-high temperature ending, it simulates the natural drying process of medicinal materials, avoids epidermal hardening caused by too rapid temperature increase, ensures uniform diffusion of internal moisture, and makes the internal moisture migration rate of medicinal materials dynamically match the surface evaporation rate through segmented temperature control, reducing the risk of cell rupture.

[0020] 3. A method for regulating the multi-barrier function of the small intestinal mucosa of early-weaned piglets. Through a three-level progressive mechanism of physical property adaptation, chemical environment regulation, and microstructure optimization in the gradient mixing process, it not only solves the technical bottleneck of multi-component mixing of compound Chinese herbal medicines, but also realizes a synergistic effect of one plus one greater than two through precise process control, providing core process support for industrial production of high-quality Chinese herbal medicine additives. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a method block diagram of the present invention;

[0022] Figure 2 It is a block diagram of the percentage of Chinese herbal medicines of the present invention;

[0023] Figure 3 It is a block diagram of segmented temperature control drying of the present invention;

[0024] Figure 4 It is a block diagram of the gradient mixing process of the present invention;

[0025] Figure 5 It is a block diagram of the specific steps of the gradient mixing process of the present invention. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0026] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0027] Please refer to Figures 1 - 5 , the present invention provides a technical solution:

[0028] Method for regulating multi-barrier function of small intestinal mucosa of early-weaned piglets by Chinese herbal medicines, wherein the Chinese herbal medicine raw materials include: 10 parts of Codonopsis pilosula, 10 parts of Atractylodes macrocephala, 10 parts of Poria cocos, 10 parts of roasted licorice root, 10 parts of Dioscorea opposita, 10 parts of Acanthopanax senticosus, 5 parts of Coix lacryma-jobi, 5 parts of Nelumbo nucifera, 5 parts of Amomum villosum, and 5 parts of malt. The regulation method is as follows:

[0029] A1. First, use an X-ray foreign object separator to remove metal impurities, and rinse with circulating pure water. After rinsing, perform centrifugal dehydration;

[0030] A2. Second, perform segmented temperature-controlled drying on the dehydrated medicinal materials;

[0031] A3. Then, put the dried medicinal materials into an air flow pulverizer for pulverization. After pulverization, irradiate with cobalt-60 γ-rays and mix them together.

[0032] Mass percentage of Chinese herbal medicine raw materials: 12-15% of Codonopsis pilosula, 10-12% of Atractylodes macrocephala, 10-12% of Poria cocos, 8-10% of roasted licorice root, 8-10% of Dioscorea opposita, 8-10% of Acanthopanax senticosus, 6-8% of Coix lacryma-jobi, 5-7% of Nelumbo nucifera, 4-6% of Amomum villosum, and 4-6% of malt.

[0033] In this embodiment, in A1, the medicinal materials are rinsed 3 times with circulating pure water, and the cleaning time for each time is 5 minutes.

[0034] Circulating pure water (conductivity ≤ 10 μS / cm) can efficiently remove sediment, eggs, pesticide residues (such as organophosphorus) and heavy metals (lead, arsenic, etc.) attached to the surface of the medicinal materials, reducing the subsequent irradiation sterilization pressure. The circulating water flow (flow rate ≥ 1.5 m / s) can avoid the growth of microorganisms caused by traditional soaking (such as a 60% reduction in the risk of Escherichia coli proliferation). Three rinses (accumulating 15 minutes) can thoroughly remove impurities in the folds and cracks of the medicinal materials through mechanical force flushing (impurity removal rate > 95%).

[0035] A single 5-minute rinse can balance the cleaning efficiency and the loss rate of water-soluble components (such as glycyrrhizic acid and polysaccharides) (the experiment shows < 0.3%); the water temperature is kept constant at 15-20 °C (regulated by a plate heat exchanger) to avoid the volatilization of volatile components (such as bornyl acetate in Amomum villosum).

[0036] Flow rinsing can reduce the total number of bacteria on the surface of the medicinal materials by 1-2 orders of magnitude (from 10 5 CFU / g to 10 3 CFU / g), reducing the subsequent irradiation dose requirement; high-pressure water flow (0.2-0.3 MPa) can destroy the biofilm structure of microorganisms on the surface of the medicinal materials (SEM observation shows that the coverage rate is reduced by 75%).

[0037] In this embodiment, the temperature for segmented temperature-controlled drying in A2 is drying at 40°C for two hours, 50°C for four hours, and 60°C for two hours.

[0038] Through the temperature gradient of starting at low temperature, transitioning at medium temperature, and ending at moderately high temperature, it simulates the natural drying process of medicinal materials, avoids the hardening of the epidermis caused by too rapid temperature increase, ensures the uniform diffusion of internal moisture, and the segmented temperature control enables the dynamic matching of the internal moisture migration rate and the surface evaporation rate of the medicinal materials, reducing the risk of cell rupture.

[0039] Drying at 40°C (2 hours): Remove free moisture

[0040] Protect thermosensitive components:

[0041] A low-temperature environment (<45°C) can maximize the retention of volatile components (such as the retention rate of bornyl acetate in Amomum villosum > 95%) and thermally unstable active substances (such as the degradation rate of flavonoid glycosides in Acanthopanax senticosus < 3%);

[0042] Break the bound water barrier:

[0043] By slowly evaporating the adsorbed water on the surface of the medicinal materials (the proportion of free water is 60 - 70%), it lays a foundation for the subsequent removal of deep-layer moisture.

[0044] Drying at 50°C (4 hours): Remove bound moisture

[0045] Gradient osmotic dehydration:

[0046] A medium-temperature environment promotes the diffusion of capillary water and part of the bound water inside the medicinal materials (the water diffusion coefficient is increased by 2.1 times), and at the same time avoids the gelatinization of starch caused by high temperature (the degree of gelatinization ≤ 5%);

[0047] Microbial inhibition:

[0048] Continuously drying at 50°C for 4 hours can reduce the spore activity of common molds (such as Aspergillus and Penicillium) by 50 - 70%.

[0049] Drying at 60°C (2 hours): Deep sterilization and final moisture control

[0050] Targeted sterilization:

[0051] Short-time high temperature (60°C × 2h) effectively kills heat-resistant pathogenic bacteria (such as the killing rate of Salmonella > 99%), and avoids the Maillard reaction caused by long-time high temperature (the browning index ΔE < 2);

[0052] Lock the final moisture:

[0053] Through the real-time feedback of the infrared moisture meter, accurately control the final water content ≤ 8%.

[0054] In this embodiment, in A3, the particle size of the crushed material is ≤ 3 mm, the rotational speed of the classification wheel is 2800 rpm, the crushing pressure is 0.8 MPa, the D50 of the finished product particle size is 45 ± 5 μm, and the specific surface area is ≥ 350 m 2 / kg.

[0055] In this embodiment, the dose rate of cobalt-60 γ-ray irradiation in A3 is 1.5 kGy / h, and the cumulative dose is 8 kGy.

[0056] In this embodiment, the mixing in A3 adopts a gradient mixing process, which is divided into basic layer mixing, functional layer addition, synergistic layer integration and overall mixing.

[0057] In this embodiment, in the basic layer mixing, Codonopsis pilosula, Atractylodes macrocephala and Poria cocos are mixed, accounting for 30% of the total mass; in the functional layer addition, Eleutherococcus senticosus, Glycyrrhiza uralensis and Dioscorea opposita are mixed, accounting for 25%, and a 5% glycerol aqueous solution is sprayed synchronously; in the synergistic layer integration, Coix lacryma-jobi, Nelumbo nucifera, Amomum villosum and Hordeum vulgare are mixed, accounting for 15%; in the overall mixing, the three-layer materials are mixed and protected by nitrogen filling.

[0058] In the basic layer mixing, all three are high-density rhizome herbs. Prior mixing can reduce the floating and stratification of subsequent light components; Codonopsis pilosula polysaccharide and Poria cocos polysaccharide form a hydrogen bond network at the molecular level, enhancing dissolution stability;

[0059] In the functional layer addition, spraying a 5% glycerol aqueous solution synchronously forms a protective film to reduce the oxidative degradation of Eleutherococcus senticosus flavonoids; glycyrrhizic acid neutralizes Dioscorea opposita mucoprotein to avoid component denaturation caused by violent local pH fluctuations;

[0060] In the synergistic layer, the volatile oil of Amomum villosum combines with Coix lacryma-jobi cellulose through the shearing force of a three-dimensional mixer to reduce the loss during high-temperature granulation; malt amylase and Nelumbo nucifera alkaloids are physically isolated to prevent hydrolysis reactions during premixing;

[0061] In the overall mixing, under nitrogen protection, through centrifugal convection compound motion, different particle size components reach molecular-level dispersion.

[0062] In this embodiment, 0.5% nano-silica is added as a flow aid during the overall mixing process.

[0063] By adding 0.5% nano-silica as a flow aid, caking of the mixed herbal powder is avoided.

[0064] Meanwhile, the content not described in detail in this specification belongs to the prior art well-known to those skilled in the art.

[0065] In summary, first, a metal impurity is removed by an X-ray foreign object sorter, and the medicinal materials are rinsed 3 times with circulating pure water, and the cleaning time for each time is 5 minutes. After rinsing, centrifugal dehydration is carried out;

[0066] Secondly, the dehydrated medicinal materials are subjected to segmented temperature-controlled drying treatment in three stages: drying at 40°C for two hours, drying at 50°C for four hours, and drying at 60°C for two hours.

[0067] Then, the dried medicinal materials are put into an air-flow pulverizer for pulverization. After pulverization, they are irradiated with cobalt-60 γ-rays, and are mixed according to the basic layer, with Codonopsis pilosula, Atractylodes macrocephala, and Poria cocos being mixed to account for 30% of the total mass; the functional layer is added with Eleutherococcus senticosus, Radix Glycyrrhizae Preparata, and Dioscorea opposita being mixed to account for 25%, and a 5% glycerol aqueous solution is sprayed synchronously; the synergistic layer is integrated with Coix lacryma-jobi, Nelumbo nucifera, Amomum villosum, and Hordeum vulgare being mixed to account for 15%. The three-layer materials are mixed together and protected by filling with nitrogen.

[0068] It should be noted that in this article, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the term "comprising", "including" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device comprising a series of elements not only includes those elements, but also includes other elements not expressly listed, or elements inherent to such process, method, article or device.

[0069] Although the embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for regulating the multi-barrier function of the small intestinal mucosa of early weaned piglets by using Chinese herbal medicine, characterized in that: The Chinese herbal medicine raw materials include: 10 parts of Codonopsis pilosula, 10 parts of Atractylodes macrocephala, 10 parts of Poria cocos, 10 parts of Radix Glycyrrhizae Radix, 10 parts of Dioscorea oppositae, 10 parts of Acanthopanax senticosus, 5 parts of Coix seeds, 5 parts of Lotus seeds, 5 parts of Amomum villosum, and 5 parts of Malt. The control method is: A1, firstly, use X-ray foreign body sorter to remove metal impurities, and then rinse with flowing pure water, and then centrifuge to dehydrate; A2, secondly, the dehydrated medicinal materials are subjected to staged temperature-controlled drying; A3, then, the dried medicinal materials are put into a jet mill for pulverization, irradiated with cobalt 60 gamma rays after pulverization, and mixed together.

2. The method for regulating the multi-barrier function of the small intestinal mucosa of early weaned piglets by using Chinese herbal medicine according to claim 1, characterized in that: The flowing pure water in A1 is circulated to rinse the medicinal materials 3 times, and each cleaning time is 5 minutes.

3. The method for regulating the multi-barrier function of the small intestinal mucosa of early weaned piglets by using Chinese herbal medicine according to claim 1, characterized in that: The temperatures for the staged temperature-controlled drying in A2 are 40° C. for two hours, 50° C. for four hours and 60° C. for two hours.

4. The method for regulating the multi-barrier function of the small intestinal mucosa of early weaned piglets by using Chinese herbal medicine according to claim 1, characterized in that: In the A3, the crushed particle size is ≤3mm, the classifying wheel speed is 2800rpm, the crushing pressure is 0.8MPa, the finished product particle size D50 = 45±5μm, and the specific surface area is ≥350m 2 / kg.

5. The method for regulating the multi-barrier function of the small intestinal mucosa of early weaned piglets by using Chinese herbal medicine according to claim 1, characterized in that: The dose rate of the A3 by cobalt 60 gamma ray irradiation was 1.5 kGy / h, and the cumulative dose was 8 kGy.

6. The method for regulating the multi-barrier function of the small intestinal mucosa of early weaned piglets by using Chinese herbal medicine according to claim 1, characterized in that: The mixing in A3 adopts a gradient mixing process, which is divided into base layer mixing, functional layer addition, synergistic layer integration and total mixing.

7. The method for regulating the multi-barrier function of the small intestinal mucosa of early weaned piglets by using Chinese herbal medicine according to claim 6, characterized in that: The base layer is mixed so that Codonopsis pilosula, Atractylodes macrocephala and Poria cocos account for 30% of the total mass, the functional layer is added so that Acanthopanax senticosus, Radix Glycyrrhizae Radix and Dioscorea opposita account for 25%, and a 5% glycerol aqueous solution is sprayed simultaneously, the synergistic layer is integrated so that Coix seeds, lotus seeds, Amomum villosum and malt account for 15%, and the total mixing mixes the three layers of materials and is protected by nitrogen.

8. The method for regulating the multi-barrier function of the small intestinal mucosa of early weaned piglets by using Chinese herbal medicine according to claim 6, characterized in that: During the total mixing process, 0.5% nano silicon dioxide is added as a flow agent.

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