Experimental platform for inhibiting marine biofouling based on parameter-adjustable ultrasonic vibration and use method thereof

Abstract

The present application relates to the technical field of marine antifouling, and particularly relates to a marine biofouling inhibition experimental platform based on parameter-adjustable ultrasonic vibration and a use method thereof. The device comprises a signal generator, a power amplifier, an oscilloscope, an ultrasonic transducer, a computer, an industrial camera, a sound intensity meter and the like. Through the cooperation of these components, the precise control of the sound field parameters of the fouling interface is realized. The industrial camera and the sound intensity meter are carried on the movable platform and connected with the computer, so that the synchronous acquisition of the sound field distribution and the biological behavior and the real-time monitoring of the motion trajectory of the fouling organisms are realized. The influence mechanism of different sound fields on the attachment behavior of marine organisms on the antifouling surface can be systematically studied. The problems that the ultrasonic parameters are difficult to be regulated and monitored in real time in the real sea experiment are effectively solved. The experimental container array composed of experimental pools is adopted, and multiple samples can be synchronously tested in a single experiment, so that the problem of insufficient laboratory breeding density is effectively solved.

Description

Technical Field

[0001] This invention relates to the field of marine antifouling technology, and in particular to an experimental platform for inhibiting marine biofouling based on parameter-adjustable ultrasonic vibration and its usage method. Background Technology

[0002] In the marine environment, marine fouling organisms pose numerous challenges. On one hand, their attachment can clog seawater pipelines and valves, reducing the effective inner diameter of the pipelines, decreasing seawater flow, and lowering heat exchange efficiency. On the other hand, the attached microorganisms secrete acidic substances, accelerating metal corrosion. Therefore, exploring effective antifouling technologies is crucial.

[0003] Ultrasonic antifouling, as an emerging green antifouling technology, has a wide range of application prospects. Its working principle involves transmitting energy into the water through high-frequency mechanical vibrations, causing changes in sound pressure or cavitation, thereby repelling or killing the eggs and larvae of fouling organisms and achieving long-term antifouling. This method boasts significant advantages such as environmental friendliness, high efficiency and broad spectrum, adjustability, continuous protection, simple maintenance, strong adaptability, and high safety. It has applications in various scenarios, including ship hull structures, seawater pipelines, offshore platforms, and marine ranches, effectively preventing marine organisms from attaching and reducing biofouling through vibration.

[0004] However, ultrasonic antifouling technology still faces some challenges in practical applications. Marine experiments suffer from difficulties in controlling ultrasonic parameters and real-time monitoring, and are also costly, hindering high-throughput experiments. Therefore, laboratory ultrasonic antifouling experiments have become an important approach to studying the effects of ultrasonic parameters on marine organisms. However, existing ultrasonic antifouling technologies have a series of shortcomings: traditional ultrasonic antifouling devices are large, making it difficult to achieve high-density fabrication of fouling organisms in the laboratory, resulting in insufficient experimental stockpiling density and an inability to obtain accurate experimental data, thus relying solely on marine experiments; traditional observation methods cannot simultaneously quantify and analyze sound field parameters and biological behavior; and there is a lack of dynamic control over the sound field distribution characteristics of the fouling interface.

[0005] In addition, ultrasonic antifouling technology is not yet perfect in preventing marine biological pollution. The research on the response of fouling organisms to different acoustic parameters is not in-depth enough. Furthermore, the limitations of traditional observation and testing methods make it difficult to observe the movement trajectory and attachment behavior of microscopic marine organisms.

[0006] Therefore, how to provide a modular acoustic-mechanical-electrical coupling testing device that integrates sound field control, behavior observation and data detection functions and is suitable for laboratory research is a technical problem that urgently needs to be solved by those skilled in the art. Summary of the Invention

[0007] To address the problems mentioned in the background section, the present invention provides an experimental platform for inhibiting marine biofouling based on parameter-adjustable ultrasonic vibration, comprising:

[0008] The ultrasonic parameter adjustment module consists of a signal generator, a power amplifier, and an oscilloscope. The signal generator generates an adjustable frequency electrical signal, which is amplified by the power amplifier to drive the ultrasonic transducer to vibrate. The oscilloscope monitors the waveform, voltage, and current peak of the electrical signal in real time to match the resonant frequency of the ultrasonic transducer.

[0009] The vibration transmission module includes an ultrasonic transducer and an experimental platform connected thereto, the experimental platform being used to convert ultrasonic vibration energy into a dynamic sound field; the signal generator is connected to a power amplifier, one end of the power amplifier being connected to an oscilloscope and the other end being connected to the ultrasonic transducer;

[0010] The experimental container consists of an experimental platform and multiple experimental pools, used to hold seawater and fouled biological samples, with the experimental pools placed on the experimental platform;

[0011] The data acquisition device includes a mobile platform on which an industrial camera or a sound intensity meter is mounted. The industrial camera is connected to a computer via a transmission interface to capture the movement trajectory of contaminated organisms in real time, and the sound intensity meter is used to measure the sound field distribution on the surface of the experimental platform.

[0012] The experimental platform is fixed to the base of the movable platform by a bracket.

[0013] Based on the above scheme, the sound intensity meter further includes a sound intensity probe and a data acquisition instrument, and the data acquisition instrument is provided with a reading panel.

[0014] Based on the above scheme, the experimental pool is further defined as a PDMS ring, the bottom of which is bonded to the experimental platform.

[0015] Based on the above scheme, the experimental platform is further made of one of the following materials: titanium alloy, pure titanium, and copper alloy.

[0016] Furthermore, based on the above scheme, the experimental platform is disc-shaped.

[0017] Based on the above scheme, the movable platform is further described as an XYZ movable platform, which is equipped with clamps and can be manually adjusted or connected to a computer for computer control.

[0018] Based on the above scheme, the ultrasonic transducer is further installed at the center below the experimental platform and connected to the experimental platform by a thread.

[0019] The present invention also provides a method for using the experimental platform for inhibiting marine biofouling based on parameter-adjustable ultrasonic vibration as described above, comprising the following steps:

[0020] S1. Arrange the experimental pools evenly on the experimental platform and add an equal amount of seawater;

[0021] S2. Start the signal generator and oscilloscope, monitor the peak values ​​of voltage and current using the oscilloscope, and adjust the frequency of the signal generator to match the natural frequency of the ultrasonic transducer.

[0022] S3. Start the power amplifier to drive the ultrasonic transducer to vibrate and generate a dynamic sound field;

[0023] S4. Clamp and move the sound intensity meter using a movable platform fixture to measure the sound intensity value in each experimental cell and record the sound field distribution data.

[0024] S5. After measurement, remove the sound intensity meter, clamp the industrial camera on the movable platform, and connect the industrial camera to the computer; add fouling organisms to the experimental tank, and observe the behavior trajectory and status of the fouling organisms through the industrial camera or computer to obtain images of the organisms' movement trajectory.

[0025] S6. Statistically count the number of surviving (N_surviving) and the number of metamorphosed (N_metamorphosed) organisms after ultrasonic treatment, and calculate the mortality rate (D) and metamorphosis rate (M) according to the following formulas:

[0026] ; ;

[0027] Wherein, N_total represents the initial number of fouling organisms;

[0028] S7. Based on experimental data, establish a quantitative relationship between sound intensity value and the mortality and metamorphosis rates of fouled organisms:

[0029] ; ;

[0030] Where α, β, γ, and δ are experimental fitting parameters, all of which are greater than 0; I This represents the sound intensity value.

[0031] S8. Based on the quantitative relationship between sound intensity value and the mortality and metamorphosis rates of fouling organisms, the comprehensive antifouling effectiveness (E) is defined as:

[0032] And w1+w2=1;

[0033] Among them, w1 and w2 are weighting coefficients used to balance the contributions of mortality rate and abnormality rate to the antifouling effectiveness.

[0034] Based on the above scheme, in step S2, the frequency of the signal generator is adjusted so that the peak values ​​of the current and voltage reach their maximum values ​​and match the natural frequency of the ultrasonic transducer; the natural frequency of the ultrasonic transducer is 20-40kHz.

[0035] Based on the above scheme, the fitting parameters are further determined by nonlinear least squares method, and the fitting error is ≤5%.

[0036] Compared with existing technologies, the experimental platform for inhibiting marine biofouling based on parameter-adjustable ultrasonic vibration provided by this invention is a modular acoustic-mechanical-electrical coupling testing system that integrates acoustic field control, behavioral observation, and data detection. It can conduct in-depth research on the attachment behavior of marine organisms on titanium alloy antifouling surfaces under the action of acoustic fields. By using a mobile platform equipped with an industrial camera and sound intensity meter, it can achieve synchronous acquisition of acoustic field distribution and biological behavior, and achieve precise control of acoustic field parameters (such as frequency, amplitude, and sound intensity) at the fouling interface. Combined with real-time monitoring of the movement trajectory of fouling organisms, it can systematically study the influence mechanism of different acoustic fields on the attachment behavior of marine organisms on antifouling surfaces. It effectively solves the problem of difficulty in controlling ultrasonic parameters and real-time monitoring in actual sea experiments. The experimental container array composed of experimental pools can test multiple samples simultaneously in a single experiment, effectively solving the problem of insufficient breeding density in the laboratory. Attached Figure Description

[0037] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0038] Figure 1 A schematic diagram of the overall structure of the experimental platform for inhibiting marine biofouling based on parameter-adjustable ultrasonic vibration provided by the present invention;

[0039] Figure 2 for Figure 1 Enlarged view of a portion of point A in the middle;

[0040] Figure 3 This is a schematic diagram of the sound intensity meter structure provided by the present invention;

[0041] Figure 4 This is a top view of the experimental platform and experimental pool provided by the present invention.

[0042] Figure label:

[0043] 1-Computer; 2-Mobile platform; 3-Industrial camera; 4-Oscilloscope; 5-Signal generator;

[0044] 6-Power amplifier; 7-Sound intensity meter; 8-Ultrasonic transducer; 9-Experimental platform; 10-Support; 11-Experimental cell. Detailed Implementation

[0045] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0046] In the description of this invention, it should be noted that the terms "center," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing the invention and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0047] To address the challenges of traditional ultrasonic antifouling devices being too bulky to conduct high-density studies of fouling organisms in the laboratory, and the inability to obtain accurate experimental data due to insufficient experimental stocking density, thus relying solely on real-sea experiments, and the difficulty of simultaneously quantifying and analyzing acoustic field parameters and biological behavior using traditional observation methods, which lack the ability to dynamically control the acoustic field distribution characteristics of the fouling interface.

[0048] Based on the above, the present invention provides an experimental device that integrates sound field control, behavior observation and data detection functions, and is suitable for laboratory research.

[0049] refer to Figure 1-4 One embodiment of the present invention provides an experimental platform for inhibiting marine biofouling based on parameter-adjustable ultrasonic vibration, comprising:

[0050] The ultrasonic parameter adjustment module consists of a signal generator 5, a power amplifier 6, and an oscilloscope 4. The signal generator 5 generates an adjustable frequency electrical signal, which is amplified by the power amplifier 6 and drives the ultrasonic transducer 8 to vibrate. The oscilloscope 4 monitors the waveform, voltage, and current peak of the electrical signal in real time to match the resonant frequency of the ultrasonic transducer 8.

[0051] The vibration transmission module includes an ultrasonic transducer 8 and an experimental platform 9 connected thereto. The experimental platform 9 is used to convert ultrasonic vibration energy into a dynamic sound field. The signal generator 5 is connected to a power amplifier 6. One end of the power amplifier 6 is connected to an oscilloscope 4, and the other end is connected to the ultrasonic transducer 8.

[0052] The experimental container consists of an experimental platform 9 and multiple experimental pools 11, which are used to hold seawater and fouled biological samples. The experimental pools 11 are placed on the experimental platform 9.

[0053] The data acquisition device includes a mobile platform 2 on which an industrial camera 3 or a sound intensity meter 7 is mounted. The industrial camera 3 is connected to a computer 1 via a transmission interface to capture the movement trajectory of contaminated organisms in real time. The sound intensity meter 7 is used to measure the sound field distribution on the surface of the experimental platform 9.

[0054] The experimental platform 9 is fixed to the base of the movable platform 2 by a bracket 10.

[0055] The experimental platform for inhibiting marine biofouling based on parameter-adjustable ultrasonic vibration provided by this invention achieves precise control of the acoustic field parameters (such as frequency, amplitude, and intensity) of the interface affected by fouling through the coordinated use of a signal generator 5, a power amplifier 6, an oscilloscope 4, an ultrasonic transducer 8, and a sound intensity meter 7. An industrial camera 3 and a sound intensity meter 7 are mounted on a movable platform 2 and connected to a computer 1 to achieve synchronous acquisition of acoustic field distribution and biological behavior, as well as real-time monitoring of the movement trajectory of fouling organisms. This allows for systematic research on the influence mechanism of different acoustic fields on the attachment behavior of marine organisms on antifouling surfaces, effectively solving the problem of difficulty in controlling ultrasonic parameters and real-time monitoring in actual sea experiments. The experimental container array composed of experimental pools 11 allows for simultaneous testing of multiple samples in a single experiment, effectively addressing the problem of insufficient aquaculture density in laboratories.

[0056] Specifically, in use, the number of experimental pools 11 that can be placed on the experimental platform 9 at one time is 3-6.

[0057] Those skilled in the art can adjust the number of experimental pools 11 according to experimental needs, including but not limited to the number provided in this embodiment.

[0058] In one embodiment, such as Figure 2 As shown, the sound intensity meter 7 includes a sound intensity probe and a data acquisition instrument, and the data acquisition instrument is equipped with a reading panel.

[0059] Specifically, a sound intensity meter 7 is used to measure the sound intensity in each experimental cell 11, and the sound intensity value is displayed on the reading panel to visualize the acoustic parameters.

[0060] In one embodiment, such as Figure 3-4 As shown, the experimental pool 11 is a PDMS ring, the bottom of which is bonded to the experimental platform 9.

[0061] Preferably, the experimental pool 11 has an inner diameter of 40 mm, an outer diameter of 60 mm, and a thickness of 20 mm.

[0062] Specifically, the PDMS ring is a circular ring, with its bottom bonded to the experimental platform 9, forming a container with the experimental platform 9 that can hold seawater and fouling organisms.

[0063] PDMS rings were used as experimental containers because PDMS rings have good biocompatibility and are easy for fouling organisms to adhere to.

[0064] Those skilled in the art can also, based on the technical concept of this embodiment, replace the PDMS ring with materials for different antifouling scenarios such as ships, pipelines or offshore platforms, according to their experimental needs, in order to experiment or monitor the fouling bioattachment under different sound pressures on different materials.

[0065] It should be noted that the PDMS is polydimethylsiloxane; the size and shape of the PDMS ring can be adjusted according to the required stocking density of each experimental pond 11 during the experiment, including but not limited to the size and shape provided in this embodiment, for example, the PDMS ring can be set as a square ring structure, a rectangular ring structure, an elliptical ring structure, etc.

[0066] In one embodiment, the experimental platform 9 is made of one of titanium alloy, pure titanium, and copper alloy.

[0067] Preferably, the experimental platform 9 is made of titanium alloy.

[0068] Titanium alloy was used as the experimental platform 9 because titanium alloy has good biocompatibility and fouling organisms can easily adhere to it.

[0069] Those skilled in the art can also, based on the technical concept of this embodiment, replace the material of the experimental platform 9 with materials from different antifouling scenarios such as ships, pipelines, or offshore platforms, according to their experimental needs, in order to experiment or monitor the fouling biofouling situation under different sound pressures on different materials.

[0070] In one embodiment, such as Figure 4 As shown, the experimental platform 9 is disk-shaped.

[0071] Preferably, the disc has a diameter of 300 mm, a thickness of 3 mm, and an inner hole diameter of 10.5 mm.

[0072] Specifically, in use, the electrical signal is converted into mechanical vibration of the experimental platform 9 by the ultrasonic transducer 8.

[0073] It should be noted that, based on the concept of this embodiment, those skilled in the art can modify the shape and size of the experimental platform 9 according to actual needs, including but not limited to the shape and size provided in this embodiment. For example, if a higher throughput experiment is required, the diameter of the experimental platform 9 can be enlarged to accommodate more PDMS rings.

[0074] In one embodiment, such as Figure 2 As shown, the movable platform 2 is an XYZ movable platform 2, which is equipped with clamps. It can be manually adjusted or connected to a computer 1 for control. The movable platform can move in the three-axis directions of X, Y and Z by computer control.

[0075] Specifically, the fixture holds an industrial camera 3 or a sound intensity meter 7. In use, the X-axis, Y-axis and Z-axis of the movable platform 2 are moved manually or controlled by a computer 1 to observe each experimental pool 11 and measure the sound intensity.

[0076] In one embodiment, such as Figure 4 As shown, the ultrasonic transducer 8 is installed at the center below the experimental platform 9 and is connected to the experimental platform 9 by a thread.

[0077] Specifically, the experimental pool 11 is placed on the experimental platform 9. Since the sound intensity of the experimental platform 9, which is excited by the ultrasonic transducer 8, weakens from the center to the surrounding area during vibration, the experimental pool 11 can be placed at different positions on the experimental platform 9 to adjust the position, distance and other parameters of the ultrasonic action, and to facilitate high-throughput experiments and improve experimental efficiency.

[0078] An embodiment of the present invention also provides a method for using the experimental platform for inhibiting marine biofouling based on parameter-adjustable ultrasonic vibration as described above, comprising the following steps:

[0079] S1. Arrange the experimental pools 11 evenly on the experimental platform 9 and add an equal amount of seawater;

[0080] S2. Start the signal generator 5 and oscilloscope 4, monitor the peak values ​​of voltage and current through the oscilloscope, and adjust the frequency of the signal generator 5 to match the natural frequency of the ultrasonic transducer 8.

[0081] S3. Start the power amplifier 6 to drive the ultrasonic transducer 8 to vibrate and generate a dynamic sound field;

[0082] S4. The sound intensity meter 7 is clamped and moved by the movable platform 2 to measure the sound intensity value in each experimental cell 11 and record the sound field distribution data.

[0083] S5. After measurement, remove the sound intensity meter 7, clamp the industrial camera 3 on the movable platform 2, turn on the computer 1 and connect to the industrial camera 3; add fouling organisms to the experimental pool 11, observe the behavior trajectory and status of the fouling organisms through the industrial camera 3 or the computer 1, and obtain images of the organisms' movement trajectory.

[0084] S6. Statistically count the number of surviving (N_surviving) and the number of metamorphosed (N_metamorphosed) organisms after ultrasonic treatment, and calculate the mortality rate (D) and metamorphosis rate (M) according to the following formulas:

[0085] ; ;

[0086] Wherein, N_total represents the initial number of fouling organisms;

[0087] S7. Based on experimental data, establish a quantitative relationship between sound intensity value and the mortality and metamorphosis rates of fouled organisms:

[0088] ; ;

[0089] Where α, β, γ, and δ are experimental fitting parameters, all of which are greater than 0; I This represents the sound intensity value.

[0090] S8. Based on the quantitative relationship between sound intensity value and the mortality and metamorphosis rates of fouling organisms, the comprehensive antifouling effectiveness (E) is defined as:

[0091] And w1+w2=1;

[0092] Among them, w1 and w2 are weighting coefficients used to balance the contributions of mortality rate and abnormality rate to the antifouling effectiveness.

[0093] Using the method provided by this invention, the ultrasonic frequency, power and other parameters can be adjusted by signal generator 5, oscilloscope 4 and power amplifier 6 to achieve precise control of the acoustic field parameters of the fouling interface. By using a mobile platform 2 equipped with an industrial camera 3 and sound intensity meter 7, and in conjunction with a computer 1, the synchronous acquisition of acoustic field distribution and biological behavior and real-time monitoring of the movement trajectory of fouling organisms can be achieved, effectively solving the problem of difficulty in adjusting ultrasonic parameters and real-time monitoring in actual sea experiments.

[0094] Furthermore, the mortality rate formula directly quantifies the killing effect of ultrasonic treatment on fouling organisms, and the metamorphosis rate formula measures the proportion of surviving larvae that have undergone metamorphosis, reflecting the long-term inhibition of the biological life cycle by ultrasound. This allows for precise assessment of antifouling efficiency at different sound intensities and frequencies. The relationship between metamorphosis rate, mortality rate, and sound intensity is described by functions, integrating mortality rate and metamorphosis rate. Weighting coefficients are used to adapt to different scenario requirements and meet the needs of complex marine environments.

[0095] When in use, the contribution of mortality rate and metamorphosis rate to antifouling efficacy can be balanced by weighting coefficients. For example, if the killing effect is emphasized, w1=0.7 and w2=0.3 can be set; if the development inhibition is emphasized, w1=0.4 and w2=0.6 can be set. The weighting coefficients can be adjusted according to actual needs, including but not limited to the scheme provided in this embodiment.

[0096] In one embodiment, in step S2, the oscilloscope 4 can display the waveform of the electrical signal, the peak values ​​of voltage and current on the screen, and adjust the frequency of the signal generator 5 so that the peak values ​​of current and voltage reach their maximum values ​​and match the natural frequency of the ultrasonic transducer 8; the natural frequency of the ultrasonic transducer 8 is 20-40kHz.

[0097] Preferably, the natural frequency of the ultrasonic transducer 8 is 40 kHz.

[0098] Specifically, the oscilloscope 4 displays the electrical signal as a waveform on the screen, and can also display the peak value and phase angle of the current and voltage of the electrical signal. By changing the frequency of the output signal, the peak value of the current and voltage can be changed. When the frequency is adjusted so that the peak value of the voltage and current reaches the maximum, the frequency of the electrical signal is matched with the natural frequency of the ultrasonic transducer 8.

[0099] In one embodiment, the fitting parameters are determined by nonlinear least squares method, and the fitting error is ≤5%.

[0100] Although this document frequently uses terms such as computer, mobile platform, industrial camera, oscilloscope, signal generator, power amplifier, sound intensity meter, ultrasonic transducer, experimental platform, support, and experimental cell, the possibility of using other terms is not excluded. These terms are used merely for the convenience of describing and explaining the essence of the invention; interpreting them as any additional limitation would contradict the spirit of the invention.

[0101] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for using an experimental platform for inhibiting marine biofouling based on parameter-adjustable ultrasonic vibration, characterized in that, The experiment is conducted using a parameter-adjustable ultrasonic vibration-based experimental platform, which includes a signal generator, power amplifier, oscilloscope, ultrasonic transducer, experimental platform and experimental cell, movable platform, and industrial camera or sound intensity meter; the method includes the following steps: S1. Arrange the experimental pools evenly on the experimental platform and add an equal amount of seawater; S2. Start the signal generator and oscilloscope, monitor the peak values ​​of voltage and current using the oscilloscope, and adjust the frequency of the signal generator to match the natural frequency of the ultrasonic transducer. S3. Start the power amplifier to drive the ultrasonic transducer to vibrate and generate a dynamic sound field; S4. Clamp and move the sound intensity meter using a movable platform fixture to measure the sound intensity value in each experimental cell and record the sound field distribution data. S5. After measurement, remove the sound intensity meter, clamp the industrial camera on the movable platform, and connect the industrial camera to the computer; add fouling organisms to the experimental tank, and observe the behavior trajectory and status of the fouling organisms through the industrial camera or computer to obtain images of the organisms' movement trajectory. S6. Statistically count the number of surviving organisms (N_survival) and the number of metamorphosed organisms (N_metamorphosis) after treatment with the ultrasonic device. Calculate the mortality rate (D) and metamorphosis rate (M) using the following formulas: ; ; Wherein, N_total represents the initial number of fouling organisms; S7. Based on experimental data, establish a quantitative relationship between sound intensity value and the mortality and metamorphosis rates of fouled organisms: ； ； Where α, β, γ, and δ are experimental fitting parameters, all of which are greater than 0; I This represents the sound intensity value. S8. Based on the quantitative relationship between sound intensity value and the mortality and metamorphosis rates of fouling organisms, the comprehensive antifouling effectiveness E is defined as: And w1+w2=1; Among them, w1 and w2 are weighting coefficients used to balance the contributions of mortality rate and abnormality rate to the antifouling effectiveness.

2. The method of using the experimental platform for inhibiting marine biofouling based on parameter-adjustable ultrasonic vibration according to claim 1, characterized in that: In step S2, the frequency of the signal generator is adjusted so that the peak values ​​of the current and voltage reach their maximum values ​​and match the natural frequency of the ultrasonic transducer; the natural frequency of the ultrasonic transducer is 20-40kHz.

3. The method of using the experimental platform for inhibiting marine biofouling based on parameter-adjustable ultrasonic vibration according to claim 1, characterized in that: The fitting parameters are determined by nonlinear least squares method, and the fitting error is ≤5%.

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