Dendrobium extract, extraction method and application thereof

Abstract

The invention belongs to the field of traditional Chinese medicine extracts, and provides a dendrobium extract as well as a preparation method and application thereof. Comprising the following preparation steps: extracting herba artemisiae scopariae with a solvent, enriching anti-bacteriostatic components in the herba artemisiae scopariae, mixing herba dendrobii with the herba artemisiae scopariae extract in proportion, extracting with a solvent, and purifying, refining, filtering and sterilizing an extracting solution through a chromatographic column, so as to prepare the herba dendrobii extract without the preservative, and the herba dendrobii extract can be applied to daily chemical products, medicines and health-care foods. And the shelf life can reach more than 24 months. The dendrobium extract prepared by the preparation method provided by the invention does not need to be added with traditional preservatives, so that the problems of irritation, allergy and the like caused by the preservatives are avoided, and the soothing and anti-inflammatory effects can also be improved.

Description

Technical Field

[0001] This application belongs to the technical field of traditional Chinese medicine extracts, and specifically relates to a dendrobium extract, an extraction method thereof, and an application thereof. Background Art

[0002] The application of dendrobium extract in cosmetics is mainly based on its natural active ingredients, such as polysaccharides, alkaloids, flavonoids, amino acids, and trace elements. Dendrobium extract has a deep moisturizing and nourishing effect. The principle is that dendrobium polysaccharide has strong hydrophilicity, can form a water-locking film to prevent water loss, and at the same time promotes the synthesis of natural moisturizing factors (NMF) in the stratum corneum. Dendrobium extract promotes barrier repair. Polysaccharides and amino acids can accelerate the proliferation of keratinocytes, strengthen the structure of the sebum membrane, and improve the barrier fragility caused by excessive cleaning or environmental stimulation. With its natural moisturizing, antioxidant, and repair characteristics, dendrobium extract has become a popular ingredient in the concept of "gentle skin care", especially suitable for consumers who pursue plant activity and skin barrier health.

[0003] Currently, most of the extraction methods for dendrobium use water extraction and alcohol precipitation method, acid / alkali extraction method, enzyme-assisted extraction, flash extraction, supercritical CO2 extraction, membrane separation technology, etc. Among these methods, due to the high polysaccharide content in dendrobium, it provides natural nutrients for the growth of microorganisms, and the microorganisms cause changes in the odor, appearance, quality, etc. of dendrobium extract, resulting in the instability of the obtained dendrobium extract. Therefore, a certain amount of preservative needs to be added to ensure the stability of dendrobium extract. However, since dendrobium extract is generally used in skin care products or foods, the preservative may cause skin irritation (skin care products) or food safety problems.

[0004] It can be seen that there is an urgent need for an extraction method that can obtain a stable dendrobium extract without adding preservatives, which reduces the safety risks such as allergy and irritation in skin care products, and while retaining the activity of dendrobium polysaccharide components, it also increases the soothing and anti-inflammatory effects. Summary of the Invention

[0005] To solve or partially solve the problems existing in the related technologies, this application provides an extraction method for dendrobium extract. This extraction method can obtain a dendrobium extract without adding preservatives, which can be used as a raw material for daily chemical products, and the shelf life can reach more than 24 months.

[0006] This application provides an extraction method for dendrobium extract on the one hand. The extraction method includes the following steps: 1) Add Artemisia capillaris Thunb. to the ethanol aqueous solution according to a solid-liquid ratio of 1:4 - 12; after reflux extraction and filtration, obtain a filtrate; The mass concentration of the ethanol aqueous solution is 30% - 90%; the time of the reflux extraction is 1 - 3 h, and the number of times is 1 - 4 times; 2) Recover the ethanol in the filtrate and concentrate it until the mass of the solid content accounts for 20% - 50% of the total mass to obtain the Artemisia capillaris extract; 3) Add the dendrobium and the Artemisia capillaris extract obtained in 2) to water for a second extraction to obtain an extract; The mass ratio of the dendrobium, the Artemisia capillaris extract to water is 1: (0.5 - 5): (3 - 10); 4) Purify the extract in 3) with a calcium phosphate gel chromatography column, elute with purified water, and collect the sample loading effluent and the eluate. Purify the sample loading effluent and the eluate with a silica gel chromatography column, elute with an ethanol aqueous solution, collect the eluate, perform vacuum concentration, and sterilize to obtain the dendrobium extract.

[0007] Furthermore, the solid - liquid ratio is 1:10 (kg / L), the mass concentration of the ethanol aqueous solution is 75%, the reflux extraction time is 2 h, and the number of times is 2 times.

[0008] Furthermore, the mass of the solid content accounts for 30% of the total mass.

[0009] Furthermore, the mass ratio of the dendrobium to the Artemisia capillaris extract is 1:1, and the mass ratio of the dendrobium to water is 1:6.

[0010] Furthermore, the method of the second extraction is heat - preservation extraction, the time is 4 h - 8 h, the number of times is 1 - 3 times; the temperature is 40°C - 75°C.

[0011] Furthermore, the temperature of the heat - preservation extraction is 50°C, the time is 6 h, and the number of times is 1 time.

[0012] Furthermore, the model of the calcium phosphate gel chromatography column is one of Bio - Gel P - 100, Bio - Gel P - 200, Bio - Gel P - 300, Bio - Gel P - 600, Bio - Gel P - 2000; The model of the silica gel chromatography column is one of SGB - 01, SGB - 02, SGB - 03, SGB - 04, SGB - 05, SGB - 06, SGB - 07, SGB - 08.

[0013] Furthermore, the concentration of the ethanol aqueous solution in 4) is 20% - 70%.

[0014] Furthermore, the temperature of the vacuum concentration is 40°C - 75°C.

[0015] Furthermore, the sterilization method is one of radiation sterilization, moist heat sterilization, and filtration sterilization.

[0016] Beneficial effects 1. The extraction method of dendrobium extract provided by the present invention enables the obtained dendrobium extract to be stored without adding preservatives and maintain stability in terms of appearance, odor, polysaccharide content, and microorganisms for 24 months.

[0017] 2. The dendrobium extract without adding preservatives provided by the present invention also avoids problems such as skin irritation and allergy caused by traditional preservatives, has a stronger soothing and anti-inflammatory effect, and lower irritation. At the same time, it also has an antioxidant effect.

[0018] 3. When the extraction is carried out in this application, the extraction temperature is optimized to 50 °C, the extraction time is 6 h, and the extraction is carried out once. Under such conditions, the odor is closest to the natural fragrance of dendrobium, and at the same time, the active ingredients - polysaccharides, p-hydroxyacetophenone, and chlorogenic acid are retained. This is because under this extraction condition, polysaccharides, p-hydroxyacetophenone, and chlorogenic acid can be extracted simultaneously without excessive degradation and inactivation of polysaccharides and chlorogenic acid.

[0019] It should be understood that the above general description and the following detailed description are only exemplary and explanatory, and cannot limit this application. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] By describing the exemplary embodiments of this application in more detail in conjunction with the accompanying drawings, the above and other objects, features, and advantages of this application will become more obvious. Among them, in the exemplary embodiments of this application, the same reference numerals generally represent the same components.

[0021] Figure 1 is the CAM diagram of the 0.9% NaCl eye irritation experiment; Figure 2 is the CAM diagram of the 0.1 mol / L NaOH eye irritation experiment; Figure 3 is the CAM diagram of the eye irritation experiment of Example 1; Figure 4 is the CAM diagram of the eye irritation experiment of Example 2; Figure 5 is the comparison diagram of the effects of Example 1 and Example 2 on the TNF-α secretion content of LPS-induced RAW264.7 cells. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0022] The embodiments of this application will be described in more detail below with reference to the accompanying drawings. Although the embodiments of this application are shown in the drawings, it should be understood that this application can be implemented in various forms and should not be limited by the embodiments described herein. On the contrary, these embodiments are provided to make this application more thorough and complete, and to fully convey the scope of this application to those skilled in the art.

[0023] Example 1 1. Experimental materials The experimental materials were all purchased from the market.

[0024] 2. Preparation of Dendrobium extract (1) Take dry Artemisia capillaris Thunb. ( Artemisia capillaris Thunb. ) and add it to an aqueous ethanol solution with a mass concentration of 75% at a mass-to-volume ratio of 1:10 (kg / L) for reflux extraction, 2 hours each time, and extract 2 times.

[0025] (2) Vacuum decompression concentrate the filtrate in step (1) to recover ethanol in the filtrate and concentrate it to a solid content of 30% (the solid component accounts for 30% of the total mass, and the remaining 70% is liquid or other volatile components (such as water, solvents, etc.)) to obtain Artemisia capillaris extract.

[0026] (3) The types of Dendrobium can be one or more of Dendrobium officinale Kimura et Migo ( Dendrobium officinale ), Dendrobium aduncum Wall. ex Lindl. ( Dendrobium aduncum ), Dendrobium hancockii Rolfe ( Dendrobium hancockii Rolfe ), Dendrobium chrysotoxum Lindl. ( Dendrobium chrysotoxum ), Dendrobium nobile Lindl. ( Dendrobium devonianum ), Dendrobium huoshanense C. Z. Tang et S. J. Cheng ( Dendrobium huoshanense ), Dendrobium thyrsiflorum Rchb. f. ( Dendrobium thyrsiflorum ), preferably Dendrobium officinale Kimura et Migo.

[0027] Mix it with Artemisia capillaris extract at a mass ratio of 1:1 and add it to water for extraction. The mass ratio of Dendrobium officinale Kimura et Migo to water is 1:6. The extraction method is extraction at a constant temperature (50 °C) for 6 hours, and extract 1 time to obtain an extract.

[0028] (4) The extract is purified by a Bio-Gel P-600 calcium phosphate gel chromatography column, eluted with purified water, and the sample loading effluent and eluate are collected. The sample loading effluent and eluate are further purified by an SGB-05 silica gel chromatography column, and gradient elution is carried out in sequence with ethanol aqueous solution concentrations of 20%, 30%, 50%, and 70%. The eluate is collected and vacuum decompression concentrated at 50 °C until there is no ethanol.

[0029] (5) Sterilize by filtering through a 0.2 μm sterilizing filter membrane to obtain a Dendrobium extract without added preservatives.

[0030] Example 2 (1) Add water to Dendrobium officinale Kimura et Migo at a mass ratio of 1:6 for extraction. The extraction method is extraction at a constant temperature (50 °C) for 6 hours, and extract 1 time to obtain an extract.

[0031] (2)The extract was purified by passing through a Bio-Gel P-600 calcium phosphate gel chromatography column, eluted with purified water, and the sample loading effluent and eluate were collected. The sample loading effluent and eluate were further purified by an SGB-05 silica gel chromatography column, gradient eluted successively with ethanol concentrations of 20%, 30%, 50%, and 70%, the eluate was collected, and concentrated under vacuum at 50 °C until there was no ethanol left.

[0032] (3)0.5% of phenoxyethanol based on the mass of the Dendrobium extract was added, and sterilization was carried out by filtering through a 0.2 μm sterilizing filter membrane to obtain a Dendrobium extract.

[0033] Comparative Example 1 A Dendrobium extract was prepared in the same way as in Example 1, except that: in step (1), the mass concentration of the ethanol aqueous solution was 30%.

[0034] Under such conditions, the extraction efficiency of p-hydroxyacetophenone and chlorogenic acid was significantly lower than that in Example 1 because the ethanol concentration was too low and the polarity of the solvent did not match the solubility of the active ingredients.

[0035] Comparative Example 2 A Dendrobium extract was prepared in the same way as in Example 1, except that: in step (1), the mass concentration of the ethanol aqueous solution was 90%.

[0036] Under such conditions, the extraction efficiency of p-hydroxyacetophenone and chlorogenic acid was significantly lower than that in Example 1 because the ethanol concentration was too high and the polarity of the solvent did not match the solubility of the active ingredients.

[0037] Comparative Example 3 A Dendrobium extract was prepared in the same way as in Example 1, except that: in step (1), the mass-to-volume ratio of Artemisia capillaris to the ethanol aqueous solution was 1:4 (kg / L).

[0038] Under such conditions, the extraction efficiency of p-hydroxyacetophenone and chlorogenic acid was low because the amount of ethanol added was insufficient, resulting in incomplete extraction of the active substances.

[0039] Comparative Example 4 A Dendrobium extract was prepared in the same way as in Example 1, except that: in step (1), the mass-to-volume ratio of Artemisia capillaris to the ethanol aqueous solution was 1:12 (kg / L).

[0040] Under such conditions, the energy consumption was high because the amount of ethanol added was high, increasing the energy consumption in the process of recovering ethanol. Moreover, the contents of polysaccharides, p-hydroxyacetophenone, and chlorogenic acid in the Dendrobium extract did not increase significantly compared with Example 1.

[0041] Comparative Example 5 A Dendrobium extract was prepared in the same way as in Example 1, except that: in step (1), the extraction time was 1 h.

[0042] Under such conditions, the extraction efficiency of p-hydroxyacetophenone and chlorogenic acid is low because the extraction time is short and the active ingredients are not fully extracted.

[0043] Comparative Example 6 A Dendrobium extract was prepared in the same manner as in Example 1, except that: the extraction time in step (1) was 3 h.

[0044] Under such conditions, the content of chlorogenic acid decreased significantly compared with that in Example 1, and the energy consumption was high.

[0045] Comparative Example 7 A Dendrobium extract was prepared in the same manner as in Example 1, except that: the extraction was carried out 1 time in step (1).

[0046] Under such conditions, the extraction efficiency of p-hydroxyacetophenone and chlorogenic acid is low because the number of extractions is small and the active substances are not fully extracted.

[0047] Comparative Example 8 A Dendrobium extract was prepared in the same manner as in Example 1, except that: the extraction was carried out 4 times in step (1).

[0048] Under such conditions, the extraction efficiency of p-hydroxyacetophenone and chlorogenic acid is low and the energy consumption is high.

[0049] Comparative Example 9 A Dendrobium extract was prepared in the same manner as in Example 1, except that: the mass of the solid content in step (2) accounted for 50% of the total mass.

[0050] Under such conditions, the energy consumption is high because the time and energy consumed in the concentration and drying process are relatively long. Moreover, the contents of polysaccharides, p-hydroxyacetophenone, and chlorogenic acid in the Dendrobium extract have not been significantly improved compared with those in Example 1.

[0051] Comparative Example 10 A Dendrobium extract was prepared in the same manner as in Example 1, except that: the mass ratio of Dendrobium officinale to Artemisia capillaris extract in step (3) was 1:0.5.

[0052] Under such conditions, the extraction efficiency of p-hydroxyacetophenone and chlorogenic acid decreased significantly compared with that in Example 1.

[0053] Comparative Example 11 A Dendrobium extract was prepared in the same manner as in Example 1, except that: the mass ratio of Dendrobium officinale to Artemisia capillaris extract in step (3) was 1:5.

[0054] Under such conditions, the polysaccharide content decreased significantly.

[0055] Comparative Example 12 A Dendrobium extract, the preparation method is the same as that of Example 1, except that: in step (3), the mass ratio of Dendrobium officinale to water is 1:3.

[0056] Under such conditions, the contents of polysaccharide, p-hydroxyacetophenone and chlorogenic acid decreased significantly compared with Example 1.

[0057] Comparative Example 13 A Dendrobium extract, the preparation method is the same as that of Example 1, except that: in step (3), the mass ratio of Dendrobium officinale to water is 1:10.

[0058] Under such conditions, the contents of polysaccharide, p-hydroxyacetophenone and chlorogenic acid decreased significantly compared with Example 1.

[0059] Comparative Example 14 A Dendrobium extract, the preparation method is the same as that of Example 1, except that: in step (3), the extraction time is 4 h.

[0060] Under such conditions, the content of chlorogenic acid decreased significantly compared with Example 1.

[0061] Comparative Example 15 A Dendrobium extract, the preparation method is the same as that of Example 1, except that: in step (3), the extraction time is 8 h.

[0062] Under such conditions, the content of chlorogenic acid decreased significantly compared with Example 1.

[0063] Comparative Example 16 A Dendrobium extract, the preparation method is the same as that of Example 1, except that: in step (3), the extraction is carried out 3 times.

[0064] Under such conditions, the contents of polysaccharide, p-hydroxyacetophenone and chlorogenic acid decreased significantly compared with Example 1.

[0065] Comparative Example 17 A Dendrobium extract, the preparation method is the same as that of Example 1, except that: in step (3), the extraction temperature is 40 °C.

[0066] Under such conditions, the contents of polysaccharide, p-hydroxyacetophenone and chlorogenic acid decreased significantly compared with Example 1.

[0067] Comparative Example 18 A Dendrobium extract, the preparation method is the same as that of Example 1, except that: in step (3), the extraction temperature is 75 °C.

[0068] Under such conditions, the content of chlorogenic acid decreased significantly compared with Example 1.

[0069] Comparative Example 19 A Dendrobium extract, the preparation method is the same as that of Example 1, except that: in step (4), the calcium phosphate gel chromatography column is Bio-Gel P-100.

[0070] Under such conditions, the contents of p-hydroxyacetophenone and chlorogenic acid are significantly decreased compared with Example 1.

[0071] Comparative Example 20 A Dendrobium extract, the preparation method is the same as that of Example 1, except that: in step (4), the calcium phosphate gel chromatography column is Bio-Gel P-2000.

[0072] Under such conditions, the contents of p-hydroxyacetophenone and chlorogenic acid are significantly decreased compared with Example 1.

[0073] Comparative Example 21 A Dendrobium extract, the preparation method is the same as that of Example 1, except that: in step (4), the silica gel chromatography column is SGB-01.

[0074] Under such conditions, the contents of p-hydroxyacetophenone and chlorogenic acid are significantly decreased compared with Example 1.

[0075] Comparative Example 22 A Dendrobium extract, the preparation method is the same as that of Example 1, except that: in step (4), the silica gel chromatography column is SGB-08.

[0076] Under such conditions, the content of chlorogenic acid is significantly decreased compared with Example 1.

[0077] Comparative Example 23 A Dendrobium extract, the preparation method is the same as that of Example 1, except that: in step (4), elution is carried out with 50% ethanol concentration.

[0078] Under such conditions, the contents of polysaccharide, p-hydroxyacetophenone and chlorogenic acid are significantly decreased compared with Example 1.

[0079] Comparative Example 24 A Dendrobium extract, the preparation method is the same as that of Example 1, except that: in step (4), the vacuum decompression concentration temperature is 75 °C.

[0080] Under such conditions, the content of chlorogenic acid is significantly decreased compared with Example 1.

[0081] Comparative Example 25 A Dendrobium extract, the preparation method is the same as that of Example 1, except that: in step (5), it is moist heat sterilization.

[0082] Under such conditions, the content of chlorogenic acid is significantly decreased compared with Example 1.

[0083] Comparative Example 26 A Dendrobium extract, the preparation method is the same as that of Example 1, except that: step (1) is to dry Artemisia scoparia ( Artemisia scoparia Waldst.et Kit. ).

[0084] Under such conditions, the contents of polysaccharide, p - hydroxyacetophenone and chlorogenic acid have no obvious difference compared with those in Example 1.

[0085] Example 3 1. Determine the Dendrobium extract (1) Determine the polysaccharide content by the phenol - sulfuric acid method Refer to the polysaccharide content detection method described in the specification of Patent - ZL202410312477.7, Example 6 for implementation.

[0086] (2) Determine the contents of p - hydroxyacetophenone and chlorogenic acid by high - performance liquid chromatography (HPLC) Refer to the high - performance liquid chromatography method described in the "Pharmacopoeia of the People's Republic of China" 2020 Edition, Part I, Vegetable Oils and Extracts - Artemisia capillaris Thunb. Extract for implementation.

[0087] 2. Detection results of polysaccharide, p - hydroxyacetophenone and chlorogenic acid in the Dendrobium extract Table 1 Contents of polysaccharide, p - hydroxyacetophenone and chlorogenic acid in Dendrobium extracts under different preparation conditions Example 4 1. Antiseptic performance test The antiseptic challenge test adopts the test method of single - strain inoculation, that is, the test samples are artificially contaminated with the bacterial suspension of each test strain, and the viable bacteria count in them is detected at regular intervals, and the effectiveness of the cosmetic antiseptic system is judged according to the change of the viable bacteria count.

[0088] The test method refers to the "Technical Guide for the Evaluation of Cosmetic Antiseptic Challenge Tests".

[0089] (1) Result determination: Table 2 Principles for the determination of cosmetic antiseptic challenge results - Judgment criterion A is applicable to products whose product formulations can effectively prevent the proliferation of microorganisms that may pose potential safety risks to consumers without considering other control factors.

[0090] - Judgment criterion B is applicable to products that have taken other effective control methods for microbial contamination through risk assessment in addition to product formulation factors, such as using specific packaging and other means to control microbial contamination.

[0091] (2) Antiseptic challenge results: The Dendrobium extracts prepared in Example 1 and Example 2 can both meet the anti-corrosion requirements.

[0092] Table 3 Anti-corrosion Challenge Test Items and Results 2. Eye Irritation Test - Chicken Embryo Chorioallantoic Membrane Test The chicken embryo chorioallantoic membrane test is an early adopted in vitro evaluation method for eye irritation. The chorioallantoic membrane (CAM) is a respiratory membrane that surrounds the chicken embryo. This test utilizes the characteristics of the intact, clear, and transparent vascular system of the chorioallantoic membrane in the mid-stage of the hatched chicken embryo. A certain amount of the test substance is directly contacted with the allantoic membrane of the chicken embryo. After acting for a period of time, the changes in the toxicity effect indicators of the chorioallantoic membrane (such as bleeding, coagulation, and vascular lysis) are observed. These indicators reflect the changes in the morphological structure, color, and permeability of blood vessels and vascular networks, as well as the phenomena of protein denaturation in the chorioallantoic membrane and its degree of damage. Then, a score is combined to evaluate the eye irritation of the test substance.

[0093] The test method refers to SN / T 2329-2009 "Chicken Embryo Chorioallantoic Membrane Test for Eye Irritation / Corrosion of Cosmetics".

[0094] (1) Result Calculation and Evaluation: Calculate the average IS score according to the following formula and judge the irritation classification according to Table 4: Note: secH (bleeding time) - The average time when bleeding starts to occur observed on the CAM membrane, in seconds (S).

[0095] secL (vascular lysis time) - The average time when vascular lysis starts to occur observed on the CAM membrane, in seconds (S).

[0096] secC (coagulation time) - The average time when coagulation starts to appear observed on the CAM membrane, in seconds (S).

[0097] Table 4. Result Evaluation of the Irritation Scoring Method (2) Eye Irritation Test Results: The calculation results of the irritation scoring method show that the IS score of the CAM test result in Example 1 is 0.07, belonging to non-irritating; the IS score of the CAM test result in Example 2 is 4.76, belonging to mild irritation.

[0098] Table 5 CAM Test Results 3. Evaluation Experiment on Soothing Efficacy LPS-induced RAW264.7 is a classic cell model for studying inflammatory factors. LPS binds to the antigen recognition receptor on the surface of macrophages, which can induce macrophages to secrete various inflammatory factors such as TNF-α, IL-1β, and IL-6. TNF-α can activate three signaling pathways of Caspase protease, JNK, and transcription factor NF-κB to achieve its cytotoxicity, antiviral, immunomodulatory, and apoptosis and other biological functions. By comparing the differences in the content of TNF-α secreted by RAW264.7 cells after administration of the model group and the test sample, the effect of the test sample on inhibiting the secretion of TNF-α is evaluated.

[0099] The test method refers to T / SHRH 034-2021 "Test Method for the Soothing Efficacy of Cosmetics - Determination of the Content of TNF-α Inflammatory Factor in Vitro - Test Method for Lipopolysaccharide-Induced Macrophage RAW264.7".

[0100] (1)Calculation of TNF-α inhibition rate Inhibition rate (%) = (1 - T / C) × 100%, where T is the average value of the TNF-α content in each sample group and C is the average value of the TNF-α content in the model group.

[0101] (2)Evaluation results of soothing efficacy The evaluation results of soothing efficacy show that the inhibition rate of Example 1 on TNF-α is significantly higher than that of the model group (P < 0.001), the positive group (P < 0.001), and Example 2 (P < 0.001), proving that the soothing efficacy of Example 1 is better than that of Example 2.

[0102] Table 6 Inhibition of test samples on the secretion of inflammatory factor TNF-α by LPS-induced RAW264.7 cells Example 5 The stability investigation tests of Example 1 and Example 2 were carried out according to the "Technical Guide for the Evaluation of Cosmetics Stability Test".

[0103] (1)Investigation conditions and time points Table 7 Setting table of stability investigation conditions (2)Results of stability investigation tests The accelerated test simulates the long-term storage effect in a short time (such as several weeks to 6 months) through extreme conditions, helping R & D personnel quickly discover potential problems. The results of the accelerated test show that the dendrobium extracts of Example 1 and Example 2 can remain stable in terms of appearance, odor, and microorganisms, but the polysaccharide content of Example 2 shows a downward trend.

[0104] The results of the long-term stability study showed that: the Dendrobium extract provided in Example 1 could maintain stability in terms of appearance, odor, polysaccharide content, and microorganisms within 24 months. However, for the Dendrobium extract provided in Example 2, there were changes in appearance and odor at 24 months, the polysaccharide content decreased, and the total number of microbial colonies increased.

[0105] Table 8 Stability Study - Accelerated Test Results Table 9 Stability Study - Long-Term Test Results Using the preparation method provided by the present invention, there is no need to add traditional cosmetics preservatives, which avoids problems such as skin irritation and allergies caused by traditional preservatives, and can also enhance the soothing effect of cosmetics.

[0106] The embodiments of the present application have been described above. The above description is exemplary and not exhaustive, and is not limited to the disclosed embodiments. Many modifications and variations are obvious to those of ordinary skill in the art without departing from the scope and spirit of the described embodiments. The selection of the terms used herein is intended to best explain the principles of the embodiments, practical applications, or improvements to the technology in the market, or to enable other ordinary skill in the art to understand the disclosed embodiments.

Claims

1. A method for extracting Dendrobium extract, characterized in that, The extraction method includes the following steps: 1) Add Artemisia capillaris into an ethanol aqueous solution at a solid-liquid ratio of 1:4 - 12 (kg / L); after reflux extraction, filter to obtain a filtrate; The mass concentration of the ethanol aqueous solution is 30% - 90%; the time for reflux extraction is 1 - 3 h, and the number of times is 1 - 4 times; 2) Recover the ethanol in the filtrate and concentrate it to a solid content accounting for 20% - 50% of the total mass to obtain an Artemisia capillaris extract; 3) Add Dendrobium officinale and the Artemisia capillaris extract obtained in 2) to water for a second extraction to obtain an extract; The mass ratio of Dendrobium officinale, Artemisia capillaris extract to water is 1:(0.5 - 5):(3 - 10); 4) Purify the extract in 3) by a calcium phosphate gel chromatography column, elute with purified water, and collect the sample loading effluent and eluate; purify the sample loading effluent and eluate by a silica gel chromatography column, elute with an ethanol aqueous solution, collect the eluate, perform vacuum concentration under reduced pressure, and sterilize to obtain a Dendrobium officinale extract.

2. The extraction method according to claim 1, wherein The solid-liquid ratio in 1) is 1:10 (kg / L), the mass concentration of the ethanol aqueous solution is 75%, the time for reflux extraction is 2 h, and the number of times is 2 times.

3. The extraction method according to claim 1, wherein The solid content in 2) accounts for 30% of the total mass.

4. The extraction method according to claim 1, characterized in that, The mass ratio of Dendrobium officinale to Artemisia capillaris extract is 1:1, and the mass ratio of Dendrobium officinale to water is 1:

6.

5. The extraction method according to claim 1, wherein The second extraction is carried out by heat preservation extraction, the time is 4 h - 8 h, the number of times is 1 - 3 times, and the temperature is 40°C - 75°C.

6. The extraction method according to claim 5, wherein, The temperature for heat preservation extraction is 50°C, extract for 6 h, and extract 1 time.

7. The extraction method according to claim 1, wherein The model of the calcium phosphate gel chromatography column is one of Bio-Gel P-100, Bio-Gel P-200, Bio-Gel P-300, Bio-Gel P-600, Bio-Gel P-2000; The model of the silica gel chromatography column is one of SGB-01, SGB-02, SGB-03, SGB-04, SGB-05, SGB-06, SGB-07, SGB-08.

8. The extraction method according to claim 1, wherein The concentration of the ethanol aqueous solution in 4) is 20% - 70%.

9. The extraction method according to claim 1, wherein The temperature for vacuum concentration under reduced pressure is 40°C - 75°C.

10. The extraction method according to claim 1, characterized in that, The sterilization method is one of radiation sterilization, moist heat sterilization, and filtration sterilization.

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