Plant composition as well as preparation method and application thereof in preparation of product with function of promoting eye health repair

Abstract

The invention relates to the technical field of biological medicine, and particularly discloses a plant composition, a preparation method thereof and application of the plant composition in preparation of a product with an effect of promoting eye health repair. The plant composition is prepared from the following raw material components: flos chrysanthemi, flos buddlejae, sambucus nigra fruits, radix angelicae sinensis, fructus forsythiae, tribulus terrestris and chrysanthellum indicum. Furthermore, the plant composition further comprises ganoderma sinensis and ginseng. Researches show that the plant composition prepared by the brand new method has a good eye protection effect, and can promote eye health repair; therefore, the compound has important application value when being used as an active ingredient for preparing a product with an eye protection effect and a product with an effect of promoting eye health repair.

Description

Technical Field

[0001] The present invention relates to the field of biopharmaceutical technology, and particularly relates to a plant composition, a preparation method thereof, and an application in the preparation of a product having the effect of promoting eye health repair. Background Art

[0002] Prolonged working, studying, driving, staying up late, watching TV or using mobile phones can cause eye health problems such as eye fatigue, dryness, and vision decline. Generally speaking, for the above-mentioned eye health problems, people usually use chemically synthesized products such as eye drops to relieve eye discomfort, but these products often only relieve symptoms temporarily and may bring side effects after long-term use. With the increasing demand of consumers for natural and healthy products, finding safe and effective eye care ingredients from nature has become a research hotspot. At present, some plant extracts have been applied to eye health products, such as green tea extract, blueberry extract, etc.

[0003] However, the effect of a single plant extract is often relatively limited and difficult to meet the diversified needs of modern people for eye health repair. Therefore, using multiple natural plants as raw materials and scientifically proportioning them to form a plant composition with synergistic effects has become the key to improving the efficacy of eye health products. Summary of the Invention

[0004] In order to overcome at least one of the technical problems existing in the prior art, the present invention provides a plant composition, a preparation method thereof, and an application in the preparation of a product having the effect of promoting eye health repair.

[0005] The technical solution of the present invention is as follows: The present invention first provides a plant composition, which comprises the following raw material components: chrysanthemum, buddleja officinalis, elderberry, angelica sinensis, forsythia suspense, tribulus terrestris, and chamomilla recutita.

[0006] The present invention provides a brand-new plant composition. Research shows that a plant composition prepared from raw materials such as chrysanthemum, buddleja officinalis, elderberry, angelica sinensis, forsythia suspense, tribulus terrestris, and chamomilla recutita has a good eye care effect and can promote eye health repair.

[0007] Preferably, the plant composition comprises the following raw material components in parts by weight: 5 - 10 parts of chrysanthemum; 5 - 10 parts of buddleja officinalis; 10 - 20 parts of elderberry; 10 - 20 parts of angelica sinensis; 5 - 10 parts of forsythia suspense; 5 - 10 parts of tribulus terrestris; 5 - 10 parts of chamomilla recutita.

[0008] Most preferably, the plant composition comprises the following raw material components in parts by weight: 8 parts of chrysanthemum; 8 parts of buddleja officinalis; 15 parts of elderberry; 15 parts of angelica sinensis; 8 parts of forsythia suspense; 8 parts of tribulus terrestris; 8 parts of chamomilla recutita.

[0009] Preferably, the plant composition further comprises Ganoderma sinense and ginseng.

[0010] The inventors surprisingly found in further research that when Ganoderma sinense and ginseng are further added simultaneously to raw materials such as chrysanthemum, buddleja flower, elderberry fruit, angelica, forsythia, tribulus terrestris, and chamomile, it can further significantly enhance the eye-protecting effect of the prepared plant composition.

[0011] The inventors also found in the research that in raw materials such as chrysanthemum, buddleja flower, elderberry fruit, angelica, forsythia, tribulus terrestris, and chamomile, Ganoderma sinense and ginseng must be further added simultaneously to further significantly enhance the eye-protecting effect of the prepared plant composition; however, in raw materials such as chrysanthemum, buddleja flower, elderberry fruit, angelica, forsythia, tribulus terrestris, and chamomile, adding only a single Ganoderma sinense or only a single ginseng cannot further significantly enhance the eye-protecting effect of the prepared plant composition.

[0012] More preferably, the plant composition further comprises the following raw material components in parts by weight: 10 - 20 parts of Ganoderma sinense; 10 - 20 parts of ginseng.

[0013] Most preferably, the plant composition further comprises the following raw material components in parts by weight: 15 parts of Ganoderma sinense; 15 parts of ginseng.

[0014] The present invention also provides a preparation method of the above plant composition, which comprises the following steps: Take dried chrysanthemum, buddleja flower, elderberry fruit, angelica, forsythia, tribulus terrestris, and chamomile, or take dried chrysanthemum, buddleja flower, elderberry fruit, angelica, forsythia, tribulus terrestris, chamomile, Ganoderma sinense, and ginseng, mix and pulverize to obtain a plant powder; then add a solvent for extraction, and after the extraction is completed, take the extract, concentrate and dry it to obtain a plant solvent extract, and take the plant solvent extract to obtain the plant composition.

[0015] Preferably, the solvent is an ethanol aqueous solution with a volume fraction of 50 - 95%.

[0016] Most preferably, the solvent is an ethanol aqueous solution with a volume fraction of 70%.

[0017] Preferably, the dosage ratio of the solvent to the plant powder is 10 - 20 L:1 Kg.

[0018] Most preferably, the dosage ratio of the solvent to the plant powder is 15 L:1 Kg.

[0019] Preferably, the preparation method further comprises the following steps: taking the plant solvent extract and further subjecting it to an HP-20 macroporous resin column. First, elute with an aqueous ethanol solution with a volume fraction of 33-37% and a volume of 3-8 times the column volume to remove impurities; then elute with an aqueous ethanol solution with a volume fraction of 51-54% and a volume of 3-8 times the column volume, collect the eluate eluted by the aqueous ethanol solution with a volume fraction of 51-54%, concentrate and dry it to obtain the plant macroporous resin eluate; taking the plant macroporous resin eluate to obtain the plant composition.

[0020] Most preferably, the preparation method further comprises the following steps: taking the plant solvent extract and further subjecting it to an HP-20 macroporous resin column. First, elute with an aqueous ethanol solution with a volume fraction of 36% and a volume of 6 times the column volume to remove impurities; then elute with an aqueous ethanol solution with a volume fraction of 52% and a volume of 6 times the column volume, collect the eluate eluted by the aqueous ethanol solution with a volume fraction of 52%, concentrate and dry it to obtain the plant macroporous resin eluate; taking the plant macroporous resin eluate to obtain the plant composition.

[0021] The inventors further found in the research that during the preparation of the plant composition, the plant composition prepared by further eluting the plant solvent extract through the above macroporous resin column has a significantly higher eye protection effect than the plant solvent extract.

[0022] In addition, the inventors need to explain that the elution conditions of the above macroporous resin column are very crucial. Only when the plant solvent extract is further eluted under the elution conditions of the macroporous resin column of the present invention can the eye protection effect of the obtained plant composition be significantly higher than that of the plant solvent extract; however, the eye protection effect of the plant composition eluted under the elution conditions of other macroporous resin columns is not significantly higher than that of the plant solvent extract.

[0023] The present invention also provides an application of the above plant composition in the preparation of a product with an eye protection effect.

[0024] The present invention also provides an application of the above plant composition in the preparation of a product with a promoting effect on eye health repair.

[0025] Preferably, the product is an eye care spray.

[0026] Beneficial effects: The present invention provides a novel plant composition and its preparation method; research shows that the plant composition prepared by the novel method of the present invention has a good eye protection effect and can promote eye health repair; therefore, using it as an active ingredient in the preparation of products with an eye protection effect and products with a promoting effect on eye health repair has important application value. Detailed implementation mode

[0027] The present invention will be further described in detail below in conjunction with specific embodiments, but the embodiments do not impose any form of limitation on the present invention.

[0028] Example 1 Preparation of Plant Composition Raw material composition by weight of plants: 8 parts of chrysanthemum; 8 parts of buddleja officinalis; 15 parts of elderberry fruit; 15 parts of angelica sinensis; 8 parts of forsythia suspense; 8 parts of tribulus terrestris; 8 parts of chamomilla recutita.

[0029] Preparation method: Take dry chrysanthemum, buddleja officinalis, elderberry fruit, angelica sinensis, forsythia suspense, tribulus terrestris and chamomilla recutita, mix and crush them to obtain plant powder; then add a solvent and carry out heating reflux extraction at 75 °C for 1.5 h. After the extraction is completed, take the extract, concentrate and dry it to obtain a plant solvent extract, and take the plant solvent extract to obtain the plant composition; Among them, the solvent is an ethanol aqueous solution with a volume fraction of 70%; the dosage ratio of the solvent to the plant powder is 15 L:1 kg.

[0030] Example 2 Preparation of Plant Composition Raw material composition by weight of plants: 8 parts of chrysanthemum; 8 parts of buddleja officinalis; 15 parts of elderberry fruit; 15 parts of angelica sinensis; 8 parts of forsythia suspense; 8 parts of tribulus terrestris; 8 parts of chamomilla recutita; 15 parts of ganoderma lucidum; 15 parts of ginseng.

[0031] Preparation method: Take dry chrysanthemum, buddleja officinalis, elderberry fruit, angelica sinensis, forsythia suspense, tribulus terrestris, chamomilla recutita, ganoderma lucidum and ginseng, mix and crush them to obtain plant powder; then add a solvent and carry out heating reflux extraction at 75 °C for 1.5 h. After the extraction is completed, take the extract, concentrate and dry it to obtain a plant solvent extract, and take the plant solvent extract to obtain the plant composition; Among them, the solvent is an ethanol aqueous solution with a volume fraction of 70%; the dosage ratio of the solvent to the plant powder is 15 L:1 kg.

[0032] Example 3 Preparation of Plant Composition Raw material composition by weight of plants: 8 parts of chrysanthemum; 8 parts of buddleja officinalis; 15 parts of elderberry fruit; 15 parts of angelica sinensis; 8 parts of forsythia suspense; 8 parts of tribulus terrestris; 8 parts of chamomilla recutita; 15 parts of ganoderma lucidum; 15 parts of ginseng.

[0033] Preparation method: (1) Take dry chrysanthemum, buddleja flower, elderberry fruit, angelica, forsythia, tribulus terrestris, chamomile, ganoderma lucidum and ginseng, mix and pulverize them to obtain plant powder; then add a solvent and carry out heating reflux extraction at 75 °C for 1.5 h. After the extraction is completed, take the extract, concentrate and dry it to obtain a plant solvent extract; wherein, the solvent is an ethanol aqueous solution with a volume fraction of 70%; the dosage ratio of the solvent to the plant powder is 15 L:1 kg; (2) Take the plant solvent extract and further load it onto an HP-20 macroporous resin column. First, elute with 6 column volumes of an ethanol aqueous solution with a volume fraction of 36% to remove impurities; then elute with 6 column volumes of an ethanol aqueous solution with a volume fraction of 52%. Collect the eluate eluted by the ethanol aqueous solution with a volume fraction of 52%, concentrate and dry it to obtain a plant macroporous resin eluate; take the plant macroporous resin eluate to obtain the plant composition.

[0034] Comparative Example 1 Preparation of Plant Composition Raw material composition in parts by weight of plants: chrysanthemum 8 parts; buddleja flower 8 parts; elderberry fruit 15 parts; angelica 15 parts; forsythia 8 parts; tribulus terrestris 8 parts; chamomile 8 parts; ganoderma lucidum 30 parts.

[0035] Preparation method: Take dry chrysanthemum, buddleja flower, elderberry fruit, angelica, forsythia, tribulus terrestris, chamomile and ganoderma lucidum, mix and pulverize them to obtain plant powder; then add a solvent and carry out heating reflux extraction at 75 °C for 1.5 h. After the extraction is completed, take the extract, concentrate and dry it to obtain a plant solvent extract, and take the plant solvent extract to obtain the plant composition; wherein, the solvent is an ethanol aqueous solution with a volume fraction of 70%; the dosage ratio of the solvent to the plant powder is 15 L:1 kg.

[0036] Comparative Example 2 Preparation of Plant Composition Raw material composition in parts by weight of plants: chrysanthemum 8 parts; buddleja flower 8 parts; elderberry fruit 15 parts; angelica 15 parts; forsythia 8 parts; tribulus terrestris 8 parts; chamomile 8 parts; ginseng 30 parts.

[0037] Preparation method: Take dry chrysanthemum, buddleja flower, elderberry fruit, angelica, forsythia, tribulus terrestris, chamomile and ginseng, mix and pulverize them to obtain plant powder; then add a solvent and carry out heating reflux extraction at 75 °C for 1.5 h. After the extraction is completed, take the extract, concentrate and dry it to obtain a plant solvent extract, and take the plant solvent extract to obtain the plant composition; wherein, the solvent is an ethanol aqueous solution with a volume fraction of 70%; the dosage ratio of the solvent to the plant powder is 15 L:1 kg.

[0038] Comparative Example 3 Preparation of Plant Composition Plant weight part raw material composition: 8 parts of chrysanthemum; 8 parts of buddleja officinalis; 15 parts of elderberry fruit; 15 parts of angelica sinensis; 8 parts of forsythia suspense; 8 parts of tribulus terrestris; 8 parts of chamomilla recutita; 15 parts of ganoderma lucidum; 15 parts of ginseng.

[0039] Preparation method: (1) Take the dried chrysanthemum, buddleja officinalis, elderberry fruit, angelica sinensis, forsythia suspense, tribulus terrestris, chamomilla recutita, ganoderma lucidum and ginseng, mix and pulverize to obtain plant powder; then add a solvent and carry out heating reflux extraction at 75 °C for 1.5 h, and after the extraction is completed, take the extract, concentrate and dry it to obtain a plant solvent extract; wherein, the solvent is an ethanol aqueous solution with a volume fraction of 70%; the dosage ratio of the solvent to the plant powder is 15 L: 1 kg; (2) Take the plant solvent extract and further load it onto an HP-20 type macroporous resin column, first elute with 6 times the column volume of an ethanol aqueous solution with a volume fraction of 10% to remove impurities; then elute with 6 times the column volume of an ethanol aqueous solution with a volume fraction of 30%, collect the eluate eluted by the ethanol aqueous solution with a volume fraction of 30%, concentrate and dry it to obtain a plant macroporous resin eluate; take the plant macroporous resin eluate to obtain the plant composition.

[0040] Comparative Example 4 Preparation of Plant Composition Plant weight part raw material composition: 8 parts of chrysanthemum; 8 parts of buddleja officinalis; 15 parts of elderberry fruit; 15 parts of angelica sinensis; 8 parts of forsythia suspense; 8 parts of tribulus terrestris; 8 parts of chamomilla recutita; 15 parts of ganoderma lucidum; 15 parts of ginseng.

[0041] Preparation method: (1) Take the dried chrysanthemum, buddleja officinalis, elderberry fruit, angelica sinensis, forsythia suspense, tribulus terrestris, chamomilla recutita, ganoderma lucidum and ginseng, mix and pulverize to obtain plant powder; then add a solvent and carry out heating reflux extraction at 75 °C for 1.5 h, and after the extraction is completed, take the extract, concentrate and dry it to obtain a plant solvent extract; wherein, the solvent is an ethanol aqueous solution with a volume fraction of 70%; the dosage ratio of the solvent to the plant powder is 15 L: 1 kg; (2) Take the plant solvent extract and further load it onto an HP-20 type macroporous resin column, first elute with 6 times the column volume of an ethanol aqueous solution with a volume fraction of 50% to remove impurities; then elute with 6 times the column volume of an ethanol aqueous solution with a volume fraction of 70%, collect the eluate eluted by the ethanol aqueous solution with a volume fraction of 70%, concentrate and dry it to obtain a plant macroporous resin eluate; take the plant macroporous resin eluate to obtain the plant composition.

[0042] Example 4 Eye Protection Experiment Ninety zebrafish embryos at 24 hpf were randomly divided into 9 groups, with 10 embryos in each group, namely experimental groups 1 - 7, a model group, and a blank control group. Among them, mycophenolate mofetil was added to the culture water of experimental groups 1 - 7 and the model group to make its concentration in the culture water 120 μg / ml. At the same time, the test plant composition was added to experimental groups 1 - 7 to make its concentration in the culture water 60 μg / ml. After culturing each group of zebrafish to 96 hpf, specific staining was performed on the apoptotic cells in the eyes of each group of zebrafish. Fluorescence images were observed with a fluorescence microscope, and the fluorescence intensity of apoptotic cells in the eyes of each group of zebrafish was statistically analyzed. The fluorescence intensity of apoptotic cells in the eyes of each group of zebrafish is shown in Table 1.

[0043] Among them, experimental groups 1 - 7 respectively tested the plant compositions prepared in Examples 1 - 3 and Comparative Examples 1 - 4.

[0044] Table 1. Experimental results of the plant composition for eye protection

[0045] It can be seen from the experimental data in Table 1 that the fluorescence intensity of apoptotic cells in the eyes of the model group is much higher than that of the blank control group. This indicates that the zebrafish eye cell apoptosis model of the present invention is successfully constructed.

[0046] It can also be seen from the experimental data in Table 1 that the fluorescence intensity of apoptotic cells in the eyes of experimental group 1 is much lower than that of the model group. This shows that the plant composition prepared from raw materials such as chrysanthemum, buddleja flower, elderberry fruit, angelica, forsythia, tribulus terrestris, and chamomile in the present invention has a good eye protection effect and can promote the repair of eye health.

[0047] It can further be seen from the experimental data in Table 1 that the fluorescence intensity of apoptotic cells in the eyes of experimental group 2 is further significantly lower than that of experimental group 1. This shows that further adding ganoderma lucidum and ginseng simultaneously to raw materials such as chrysanthemum, buddleja flower, elderberry fruit, angelica, forsythia, tribulus terrestris, and chamomile can further significantly improve the eye protection effect of the prepared plant composition. In addition, it can be seen from the experimental data in Table 1 that although the fluorescence intensity of apoptotic cells in the eyes of experimental groups 4 and 5 is lower than that of experimental group 1, the decrease is not significant. This shows that in order to further significantly improve the eye protection effect of the prepared plant composition, ganoderma lucidum and ginseng must be further added simultaneously to raw materials such as chrysanthemum, buddleja flower, elderberry fruit, angelica, forsythia, tribulus terrestris, and chamomile. However, adding only a single ganoderma lucidum or only a single ginseng to raw materials such as chrysanthemum, buddleja flower, elderberry fruit, angelica, forsythia, tribulus terrestris, and chamomile cannot further significantly improve the eye protection effect of the prepared plant composition.

[0048] It can also be seen from the experimental data in Table 1 that the fluorescence intensity of apoptotic cells in the eyes of Experimental Group 3 is further significantly lower than that of Experimental Group 2 and is also significantly lower than that of the blank control group. This indicates that: in the preparation process of the plant composition, the plant composition prepared by further eluting the plant solvent extract through the macroporous resin column of the present invention has a significantly higher eye protection effect than the plant solvent extract; it has an excellent eye protection effect and a promoting effect on eye health repair.

[0049] It can also be seen from the experimental data in Table 1 that, compared with Experimental Group 2, although the fluorescence intensity of apoptotic cells in the eyes of Experimental Groups 6 and 7 has decreased, the decrease amplitude is not large; the decrease amplitude is much lower than that of Experimental Group 3. This indicates that: the elution conditions of the macroporous resin column of the present invention are very crucial. Only when the plant solvent extract is further eluted under the elution conditions of the above-mentioned macroporous resin column of the present invention can the plant composition obtained have a significantly higher eye protection effect than the plant solvent extract and can be further significantly improved; however, for the plant composition eluted under the elution conditions of other macroporous resin columns, its eye protection effect cannot be significantly higher than that of the plant solvent extract and cannot be further significantly improved.

Claims

1. A plant composition, characterized in that: The invention comprises the following raw materials: chrysanthemum, buddleja, elderberry, angelica, forsythia, tribulus terrestris and golden chamomile.

2. The plant composition according to claim 1, characterized in that The invention comprises the following raw material components in parts by weight: 5-10 parts of chrysanthemum, 5-10 parts of buddlejac, 10-20 parts of elderberry, 10-20 parts of angelica, 5-10 parts of forsythia, 5-10 parts of tribulus terrestris and 5-10 parts of golden chamomile.

3. The plant composition according to claim 1, characterized in that The invention comprises the following raw material components in parts by weight: 8 parts of chrysanthemum, 8 parts of buddlejac, 15 parts of elderberry, 15 parts of angelica, 8 parts of forsythia, 8 parts of tribulus terrestris and 8 parts of golden chamomile.

4. The plant composition according to claim 1, characterized in that Also contains Ganoderma lucidum and Ginseng.

5. The plant composition according to claim 4, characterized in that It also contains the following raw material components in parts by weight: 10-20 parts of Ganoderma lucidum; 10-20 parts of ginseng; Most preferably, the plant composition further comprises the following raw material components in parts by weight: 15 parts of Ganoderma lucidum; 15 parts of ginseng.

6. The method for preparing the plant composition according to any one of claims 1 to 5, characterized in that: The following steps are included: Take dried chrysanthemum, buddleja, elderberry, angelica, forsythia, tribulus and golden chamomile, or take dried chrysanthemum, buddleja, elderberry, angelica, forsythia, tribulus, golden chamomile, Ganoderma lucidum and ginseng, mix and grind to obtain plant powder; then add solvent for extraction, after the extraction is completed, take the extract and concentrate and dry to obtain a plant solvent extract, and take the plant solvent extract to obtain the plant composition.

7. The preparation method according to claim 6, characterized in that: The solvent is an ethanol aqueous solution with a volume fraction of 50-95%; Most preferably, the solvent is an ethanol aqueous solution with a volume fraction of 70%.

8. The preparation method according to claim 6, characterized in that: The ratio of solvent to plant powder is 10~20L:1Kg; Most preferably, the ratio of solvent to plant powder is 15L:1Kg.

9. Use of the plant composition according to any one of claims 1 to 5 in preparing a product having an eye-protecting effect.

10. Use of the plant composition according to any one of claims 1 to 5 in the preparation of a product having the function of promoting eye health and repairing.

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