Wood frog collagen capable of increasing skin elasticity and resisting aging and preparation method of wood frog collagen

Abstract

The invention discloses wood frog collagen capable of increasing skin elasticity and resisting aging and a preparation method of the wood frog collagen. The antioxidant wood frog skin collagen product comprises wood frog skin collagen peptide powder, astragalus polysaccharide, centella asiatica extract, sodium hyaluronate, lycium barbarum polysaccharide and green tea extract catechin. The collagen product is rich in various nutritional ingredients, has a relatively excellent antioxidant function, can be used for improving the barrier function of skin to external harmful substances, and can continuously improve the moisture and elasticity of the skin.

Description

Technical Field

[0001] The present invention relates to the technical field of collagen products, and particularly relates to a Rana chensinensis collagen for increasing skin elasticity and anti-aging and a preparation method thereof. Background Art

[0002] As a by-product after processing Rana chensinensis oil, Rana chensinensis skin has been mostly discarded in the past. At present, through extraction technology, the efficient utilization of resources can be realized, and resource waste can be reduced; Rana chensinensis skin can be used as a natural resource with rich bioactive components and applied to fields such as biotechnology, medicine, and cosmetics. Rana chensinensis skin contains a variety of bioactive components, including: antibacterial peptides, which have a broad-spectrum antibacterial effect and have a strong inhibitory ability against a variety of pathogenic microorganisms (such as Staphylococcus aureus, Escherichia coli, etc.); hyaluronic acid (HA), which has good moisturizing properties and is widely used in the fields of medicine, food, and cosmetics; collagen, which can be used to prepare medical wound dressings and tissue-engineered skin and has good biocompatibility and mechanical properties; polypeptide substances, which have a variety of biological activities such as antibacterial, antiviral, and antioxidant.

[0003] Antioxidant collagen products have significant antioxidant and anti-aging effects and can be used in skin care products and cosmetics; however, existing products still have problems such as poor antioxidant effects, and the product stability and persistence cannot meet the requirements.

[0004] Therefore, it is necessary to develop an antioxidant collagen product containing Rana chensinensis skin and rich in various nutrients. Summary of the Invention

[0005] To solve the above technical problems, the purpose of the present invention is to provide a Rana chensinensis collagen for increasing skin elasticity and anti-aging and a preparation method thereof. This collagen product is rich in various nutrients, has excellent antioxidant functions, can be used to improve the barrier function of the skin against external harmful substances, and can continuously improve skin moisture and elasticity.

[0006] To achieve the above technical purposes and reach the above technical effects, the present invention is realized through the following technical solutions:

[0007] On the one hand, the present invention provides a Rana chensinensis collagen for increasing skin elasticity and anti-aging, which includes Rana chensinensis skin collagen peptide powder, astragalus polysaccharide, asiatic pennywort extract, sodium hyaluronate, wolfberry polysaccharide, and green tea extract catechin.

[0008] Furthermore, this collagen product includes the following components in parts by mass: 20-35 parts of Rana chensinensis skin collagen peptide powder, 3-8 parts of astragalus polysaccharide, 2-5 parts of asiatic pennywort extract, 1-3 parts of sodium hyaluronate, 3-8 parts of wolfberry polysaccharide, and 1-3 parts of green tea extract catechin.

[0009] More preferably, the collagen product comprises the following components in parts by mass: 25 parts of Rana temporaria skin collagen peptide powder, 5 parts of astragalus polysaccharide, 2 parts of centella asiatica extract, 1 part of sodium hyaluronate, 5 parts of wolfberry polysaccharide, and 2 parts of catechins from green tea extract.

[0010] Furthermore, the collagen product is in the form of powder, ointment, pill or capsule.

[0011] On the other hand, the present invention provides a method for preparing Rana temporaria collagen for enhancing skin elasticity and anti-aging, comprising the following steps:

[0012] (1) Drying, defatting and pulverizing fresh Rana temporaria skin to obtain defatted Rana temporaria skin powder;

[0013] (2) Mixing the defatted Rana temporaria skin powder with water, adjusting the pH, adding alkaline protease, and carrying out an enzymatic hydrolysis reaction. After the reaction is completed, enzyme inactivation, filtration, decolorization and re-filtration are carried out to obtain a clarified filtrate rich in collagen peptides, which is obtained as Rana temporaria skin collagen peptide powder after spray drying or freeze drying;

[0014] (3) Mixing the Rana temporaria skin collagen peptide powder, astragalus polysaccharide, centella asiatica extract, sodium hyaluronate, wolfberry polysaccharide, and catechins from green tea extract in proportion by mass, obtaining a composition, packaging the composition as a powder product, or packaging it as an ointment, pill or capsule product after making it into such forms.

[0015] Furthermore, in step (2), the mixture of defatted Rana temporaria skin powder and water is treated by ultrasonic waves.

[0016] Furthermore, in step (2), the pH is adjusted to 8 - 10.

[0017] Furthermore, in step (2), the dosage of alkaline protease is 0.5 - 1.5% of the weight of the defatted Rana temporaria skin powder.

[0018] The beneficial effects of the present invention are:

[0019] (1) The collagen peptide from the skin of Rana temporaria contains various amino acid residues. Some amino acids such as histidine, tyrosine, and tryptophan have the ability to provide protons or electrons and can react with reactive oxygen species (ROS) such as superoxide anion radicals and hydroxyl radicals, reducing them and thus scavenging free radicals. Free radicals can attack biomacromolecules within cells, such as lipids, proteins, and DNA, leading to cell damage and aging. By scavenging free radicals, the collagen peptide from the skin of Rana temporaria can reduce the damage caused by oxidative stress to cells and protect the normal functions of cells. The collagen peptide from the skin of Rana temporaria can activate antioxidant enzymes within cells, such as superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT). These enzymes can work together to convert the excessive ROS produced in the body into harmless substances and maintain the redox balance within cells. For example, SOD can convert superoxide anion radicals into hydrogen peroxide, while CAT and GSH-Px further decompose hydrogen peroxide into water and oxygen, thereby reducing the damage of oxidative stress to cells.

[0020] (2) Astragalus polysaccharide contains rich active groups. These groups can react with reactive oxygen species (ROS) such as superoxide anion radicals, hydroxyl radicals, and hydrogen peroxide, and reduce free radicals by providing electrons or protons, thereby achieving the purpose of scavenging free radicals. For example, the hydroxyl groups on some polysaccharide chains in Astragalus polysaccharide can directly combine with free radicals, making them lose their activity and reducing the oxidative damage of free radicals to biomacromolecules within cells (such as lipids, proteins, and DNA), and maintaining the normal physiological functions of cells. Astragalus polysaccharide can provide nutrients for skin cells and promote the proliferation and differentiation of skin cells (such as keratinocytes, fibroblasts, etc.). For damaged skin cells, Astragalus polysaccharide can accelerate their repair process. By regulating the expression of cell cycle-related proteins, it can prompt cells to enter the proliferation phase from the stationary phase, accelerating the renewal and repair of skin tissues. For example, during the process of skin wound healing, Astragalus polysaccharide can promote fibroblasts to synthesize and secrete collagen and elastic fibers, enhancing the toughness and elasticity of the skin and promoting wound healing. Astragalus polysaccharide has certain moisturizing properties. The polysaccharide chains in its molecular structure contain a large number of hydrophilic groups (such as hydroxyl groups, carboxyl groups, etc.). These groups can combine with water molecules to form a hydration layer, increasing the water content of the skin.

[0021] (3) Components such as asiaticoside and madecassoside in centella asiatica extract can provide hydrogen atoms or electrons, react with reactive oxygen species (ROS) such as superoxide anion radicals, hydroxyl radicals, and hydrogen peroxide, and reduce them to stable molecules, thereby reducing the damage of free radicals to cells. These free radicals will attack lipids, proteins, and DNA within cells, leading to cell dysfunction and aging. By scavenging free radicals, centella asiatica extract can protect the normal physiological functions of cells. Centella asiatica extract has a positive effect on fibroblasts in the skin and can promote the synthesis and secretion of collagen by fibroblasts. It can up-regulate the expression of collagen-related genes and increase the synthesis of type I and type III collagen. Collagen is the main structural protein of the skin and is crucial for maintaining the elasticity and firmness of the skin. With age or under the influence of external environmental factors, collagen in the skin gradually loses, resulting in problems such as skin laxity and wrinkles. By promoting collagen synthesis, centella asiatica extract helps improve the elasticity and texture of the skin and reduces the formation of wrinkles. Centella asiatica extract can also promote the synthesis of lipid components such as ceramides in the skin. These lipids are important components of the skin barrier, help maintain the normal barrier function of the skin, prevent the invasion of external harmful substances, and protect the skin from irritation and damage.

[0022] (4) Catechins have strong antioxidant activity and can scavenge various free radicals such as hydroxyl radicals and superoxide anion radicals. It can interact with small peptides to form stable complexes and enhance the antioxidant properties of small peptides. At the same time, catechins can also regulate the expression of antioxidant-related genes in cells and further improve the antioxidant ability.

[0023] (5) Lycium barbarum polysaccharide can provide nutritional support for skin cells (such as keratinocytes, fibroblasts, etc.) and promote cell proliferation and differentiation. When the skin is damaged or in an aging state, the proliferation and repair ability of cells decreases, and Lycium barbarum polysaccharide can accelerate the renewal and repair process of skin cells. For example, it can promote fibroblasts to synthesize and secrete collagen and elastic fibers, enhance the toughness and elasticity of the skin, reduce the formation of wrinkles, and at the same time accelerate the healing of damaged skin tissues, keeping the skin smooth and firm. Lycium barbarum polysaccharide has certain moisturizing properties. The polysaccharide chains in its molecular structure contain a large number of hydrophilic groups (such as hydroxyl groups, carboxyl groups, etc.). These groups can combine with water molecules to form a hydration layer, increasing the water content of the skin. In addition, it can also regulate the synthesis and metabolism of natural moisturizing factors (such as hyaluronic acid, ceramide, etc.) in the skin cutin layer, further enhancing the moisturizing ability of the skin, keeping the skin moist, improving the skin barrier function, and reducing the invasion of external harmful substances to the skin.

[0024] (6) Sodium hyaluronate is an efficient moisturizer with a powerful water-absorbing ability. A single molecule of sodium hyaluronate can bind hundreds or even thousands of times its own weight in water molecules. When taken orally, sodium hyaluronate can exert its effects in the body, increasing the water content of the skin's stratum corneum and improving the skin's hydration state. Sodium hyaluronate helps maintain and repair the skin's barrier function. It can promote the synthesis and arrangement of lipids in the skin's stratum corneum, enhance the tight junctions between skin cells, reduce skin water loss, and prevent the invasion of external harmful substances (such as ultraviolet rays, pollutants, bacteria, etc.). A healthy skin barrier can reduce the irritation of external factors on the skin and lower the probability of oxidative stress. At the same time, a good skin barrier function is also conducive to the normal operation of the skin's own antioxidant mechanism, enabling the skin to better resist free radical damage.

[0025] This collagen product is rich in various nutrients and has excellent antioxidant functions. It can be used to improve the skin's barrier function against external harmful substances and continuously improve skin moisture and elasticity. Detailed implementation mode

[0026] The technical solutions in the present invention will be clearly and completely described below in conjunction with specific embodiments. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0027] The present invention provides a Rana chensinensis collagen for increasing skin elasticity and anti-aging, which includes Rana chensinensis skin collagen peptide powder, astragalus polysaccharide, centella asiatica extract, sodium hyaluronate, wolfberry polysaccharide, and green tea extract catechin.

[0028] This collagen product includes the following components by mass fraction: 20 - 35 parts of Rana chensinensis skin collagen peptide powder, 3 - 8 parts of astragalus polysaccharide, 2 - 5 parts of centella asiatica extract, 1 - 3 parts of sodium hyaluronate, 3 - 8 parts of wolfberry polysaccharide, and 1 - 3 parts of green tea extract catechin.

[0029] Preferably, this collagen product includes the following components by mass fraction: 25 parts of Rana chensinensis skin collagen peptide powder, 5 parts of astragalus polysaccharide, 2 parts of centella asiatica extract, 1 part of sodium hyaluronate, 5 parts of wolfberry polysaccharide, and 2 parts of green tea extract catechin.

[0030] This collagen product is in the form of powder, ointment, pill, or capsule.

[0031] The preparation method of this Rana chensinensis collagen includes the following steps:

[0032] (1) Dry, degrease and pulverize fresh Rana temporaria chensinensis skin to obtain defatted Rana temporaria chensinensis skin powder; through degreasing treatment, effectively remove excess oil and impurities; after pulverization, the Rana temporaria chensinensis skin can also be soaked in clean water for a long time to make it fully absorb water and soften; then carry out multiple rounds of meticulous cleaning until the washing liquid is clear and transparent to ensure the absolute cleanliness of the raw materials;

[0033] (2) Mix the defatted Rana temporaria chensinensis skin powder with water at a mixing ratio of 1:(2 - 3), then treat it with 110 kHz ultrasonic wave, adjust the pH to 8 - 10, add alkaline protease, and the dosage of alkaline protease is 0.5 - 1.5% of the weight (wet weight) of the defatted Rana temporaria chensinensis skin powder, carry out enzymatic hydrolysis reaction for 4 - 6 hours, after the reaction ends, inactivate the enzyme by heating at high temperature for 10 minutes to terminate the reaction, then carry out fine filtration to remove all solid residues to obtain a clarified filtrate rich in collagen peptides; add an appropriate amount of activated carbon (about 0.3 - 0.6% of the liquid weight) to the filtrate, stir for 45 minutes under suitable conditions (50 °C, pH 6.0) to effectively remove pigment and fishy smell components; after filtering to remove the activated carbon, obtain a purer solution, and then obtain Rana temporaria chensinensis skin collagen peptide powder after spray drying or freeze drying;

[0034] (3) Mix the Rana temporaria chensinensis skin collagen peptide powder, astragalus polysaccharide, centella asiatica extract, sodium hyaluronate, wolfberry polysaccharide, and catechin of green tea extract evenly according to the mass parts ratio to obtain a composition, package the composition as a powder finished product, or make it into an ointment, pill or capsule and then package it as a finished product.

[0035] Example 1

[0036] The present invention provides a Rana temporaria chensinensis collagen for increasing skin elasticity and anti - aging, which includes the following components according to mass parts: 20 parts of Rana temporaria chensinensis skin collagen peptide powder, 3 parts of astragalus polysaccharide, 2 parts of centella asiatica extract, 2 parts of sodium hyaluronate, 3 parts of wolfberry polysaccharide, and 2 parts of catechin of green tea extract.

[0037] The collagen product of this Example 1 is a powder.

[0038] The preparation method of this collagen product includes the following steps:

[0039] (1) Dry, degrease and pulverize fresh Rana temporaria chensinensis skin to obtain defatted Rana temporaria chensinensis skin powder; through degreasing treatment, effectively remove excess oil and impurities; after pulverization, soak the Rana temporaria chensinensis skin in clean water for 24 h to make it fully absorb water and soften; then carry out multiple rounds of meticulous cleaning until the washing liquid is clear and transparent to ensure the absolute cleanliness of the raw materials;

[0040] (2) Mix the defatted Rana temporaria skin powder with water at a mixing ratio of 1:3, then treat it with ultrasonic waves at 110 kHz. After adjusting the pH to 9, add alkaline protease. The dosage of alkaline protease is 1% of the weight (wet weight) of the defatted Rana temporaria skin powder, and carry out an enzymatic hydrolysis reaction for 4 hours. After the reaction is completed, inactivate the enzyme by heating at a high temperature (100 °C) for 10 minutes to terminate the reaction. Subsequently, perform fine filtration to remove all solid residues and obtain a clarified filtrate rich in collagen peptides; add an appropriate amount of activated carbon (about 0.5% of the liquid weight) to the filtrate, stir at appropriate conditions (50 °C, pH 6.0) for 45 minutes to effectively remove pigment and fishy smell components; after filtering to remove the activated carbon, obtain a purer solution, then put the Rana temporaria skin collagen peptide filtrate into a suitable container, place it in the pre-freezing chamber (-40 °C to -60 °C) of a freeze dryer for 6 hours, and then perform vacuum drying for 24 hours to obtain Rana temporaria skin collagen peptide powder;

[0041] (3) Mix the Rana temporaria skin collagen peptide powder, astragalus polysaccharide, centella asiatica extract, sodium hyaluronate, wolfberry polysaccharide, and catechin of green tea extract evenly according to the mass parts ratio to obtain a composition, and package the composition as a powder finished product.

[0042] Example 2

[0043] The present invention provides a Rana temporaria collagen for increasing skin elasticity and anti-aging, which includes the following components according to the mass parts: 25 parts of Rana temporaria skin collagen peptide powder, 5 parts of astragalus polysaccharide, 2 parts of centella asiatica extract, 1 part of sodium hyaluronate, 5 parts of wolfberry polysaccharide, and 2 parts of catechin of green tea extract.

[0044] The collagen product of this Example 2 is a powder.

[0045] The preparation method of this collagen product includes the following steps:

[0046] (1) Dry and defat fresh Rana temporaria skin and then crush it to obtain defatted Rana temporaria skin powder; through the defatting treatment, effectively remove excess oil and impurities; after crushing, the Rana temporaria skin can also be soaked in clean water for 24 h to make it fully absorb water and soften; then carry out multiple rounds of careful washing until the washing liquid is clear and transparent to ensure the absolute cleanliness of the raw materials;

[0047] (2) Mix the defatted Rana temporaria skin powder with water at a mixing ratio of 1:2.5, then treat it with ultrasonic waves at 110 kHz. After adjusting the pH to 8, add alkaline protease, and the dosage of alkaline protease is 1% of the weight (wet weight) of the defatted Rana temporaria skin powder. Conduct an enzymatic hydrolysis reaction for 4 hours. After the reaction ends, inactivate the enzyme by heating at a high temperature (100 °C) for 10 minutes to terminate the reaction. Subsequently, conduct fine filtration to remove all solid residues to obtain a clarified filtrate rich in collagen peptides; add an appropriate amount of activated carbon (about 0.5% of the liquid weight) to the filtrate, stir at appropriate conditions (50 °C, pH 6.0) for 45 minutes to effectively remove pigment and fishy smell components; after filtering to remove the activated carbon, obtain a purer solution, then fill the Rana temporaria skin collagen peptide filtrate into a suitable container, place it in the pre-freezing chamber (-40 °C to -60 °C) of a freeze dryer for 6 hours, and then conduct vacuum drying for 24 hours to obtain Rana temporaria skin collagen peptide powder;

[0048] (3) Mix the Rana temporaria skin collagen peptide powder, astragalus polysaccharide, asiatic pennywort extract, sodium hyaluronate, wolfberry polysaccharide, and catechin of green tea extract evenly according to the mass parts ratio to obtain a composition, and package the composition as a powder finished product.

[0049] Example 3

[0050] The present invention provides a Rana temporaria collagen for increasing skin elasticity and anti-aging, which includes the following components according to mass parts: 30 parts of Rana temporaria skin collagen peptide powder, 5 parts of astragalus polysaccharide, 5 parts of asiatic pennywort extract, 2 parts of sodium hyaluronate, 5 parts of wolfberry polysaccharide, and 2 parts of catechin of green tea extract.

[0051] The collagen product of this Example 3 is a powder.

[0052] The preparation method of this collagen product includes the following steps:

[0053] (1) Dry and defat the fresh Rana temporaria skin and then crush it to obtain defatted Rana temporaria skin powder; through the defatting treatment, effectively remove excess oil and impurities; after crushing, the Rana temporaria skin can also be soaked in clear water for 24 h to make it fully absorb water and soften; subsequently, conduct multiple rounds of meticulous cleaning until the washing liquid is clear and transparent to ensure the absolute cleanliness of the raw materials;

[0054] (2) Mix the defatted Rana temporaria skin powder with water at a mixing ratio of 1:3, then treat it with ultrasonic waves at 110 kHz. After adjusting the pH to 9, add alkaline protease, and the dosage of alkaline protease is 1% of the weight (wet weight) of the defatted Rana temporaria skin powder. Conduct an enzymatic hydrolysis reaction for 4 hours. After the reaction ends, inactivate the enzyme by heating at a high temperature (100 °C) for 10 minutes to terminate the reaction. Subsequently, perform fine filtration to remove all solid residues and obtain a clear filtrate rich in collagen peptides; add an appropriate amount of activated carbon (about 0.5% of the liquid weight) to the filtrate, stir for 45 minutes under suitable conditions (50 °C, pH 6.0) to effectively remove pigment and fishy smell components; after filtering to remove the activated carbon, obtain a purer solution, and then prepare the Rana temporaria skin collagen peptide powder by spray drying the Rana temporaria skin collagen peptide filtrate through a spray dryer using the spray drying process;

[0055] (3) Mix the Rana temporaria skin collagen peptide powder, astragalus polysaccharide, asiatic pennywort extract, sodium hyaluronate, wolfberry polysaccharide, and catechin of green tea extract evenly according to the mass fraction ratio to obtain a composition, and package the composition as a powder finished product.

[0056] For those skilled in the art, it is obvious that the present invention is not limited to the details of the above exemplary embodiments, and the present invention can be implemented in other specific forms without departing from the spirit or basic characteristics of the present invention. Therefore, from any point of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the present invention is defined by the appended claims rather than the above description. Therefore, all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be included in the present invention.

[0057] In addition, it should be understood that although this specification is described according to embodiments, not every embodiment only contains an independent technical solution. This narrative way of the specification is only for clarity. Those skilled in the art should regard the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A Rana temporaria collagen that increases skin elasticity and resists aging, characterized in that, It includes Rana chensinensis skin collagen peptide powder, astragalus polysaccharide, asiatic pennywort extract, sodium hyaluronate, wolfberry polysaccharide and green tea extract catechin.

2. The Rana temporaria collagen for increasing skin elasticity and anti-aging according to claim 1, characterized in that The collagen product comprises the following components by mass parts: 20-35 parts of Rana chensinensis skin collagen peptide powder, 3-8 parts of astragalus polysaccharide, 2-5 parts of asiatic pennywort extract, 1-3 parts of sodium hyaluronate, 3-8 parts of wolfberry polysaccharide, and 1-3 parts of green tea extract catechin.

3. The Rana chensinensis collagen for increasing skin elasticity and anti-aging according to claim 2, characterized in that The collagen product comprises the following components by mass parts: 25 parts of Rana chensinensis skin collagen peptide powder, 5 parts of astragalus polysaccharide, 2 parts of asiatic pennywort extract, 1 part of sodium hyaluronate, 5 parts of wolfberry polysaccharide, and 2 parts of green tea extract catechin.

4. A Rana temporaria collagen for increasing skin elasticity and anti-aging according to claim 1, characterized in that, The collagen product is in the form of powder, paste, pill or capsule.

5. A preparation method of Rana temporaria collagen for increasing skin elasticity and anti-aging as described in any one of claims 1-4, characterized in that, It includes the following steps: (1) Dry and degrease fresh Rana chensinensis skin and then crush it to obtain defatted Rana chensinensis skin powder; (2) Mix the defatted Rana chensinensis skin powder with water, adjust the pH, add alkaline protease, and carry out an enzymatic hydrolysis reaction. After the reaction ends, inactivate the enzyme, filter, decolorize, and filter again to obtain a clarified filtrate rich in collagen peptides, and obtain Rana chensinensis skin collagen peptide powder after spray drying or freeze drying; (3) Mix the Rana chensinensis skin collagen peptide powder, astragalus polysaccharide, asiatic pennywort extract, sodium hyaluronate, wolfberry polysaccharide, and green tea extract catechin evenly according to the mass parts ratio to obtain a composition, package the composition as a powder finished product, or make it into a paste, pill or capsule and then package it as a finished product.

6. The preparation method of Rana temporaria collagen for increasing skin elasticity and anti-aging according to claim 5, characterized in that In step (2), the defatted Rana chensinensis skin powder and water are mixed and then treated by ultrasonic wave.

7. The preparation method of Rana temporaria collagen for increasing skin elasticity and anti-aging according to claim 5, characterized in that, In step (2), the pH is adjusted to 8-10.

8. The preparation method of Rana temporaria collagen for increasing skin elasticity and anti-aging according to claim 5, characterized in that, In step (2), the dosage of alkaline protease is 0.5-1.5% of the weight of the defatted Rana chensinensis skin powder.