Multifunctional pipetting module based on cell culture

Abstract

The invention relates to the technical field of pipetting, in particular to a multifunctional pipetting module based on cell culture, which comprises a pipetting module, a combined frame and a culture base, a plurality of mounting grooves are formed in the upper surface of the culture base, movable bases are rotatably connected in the mounting grooves, and a plurality of pipetting heads are arranged at the bottom of the pipetting module. One end of the rotating shaft on the movable base extends out of the culture base, and a first overturning assembly is fixedly arranged at the end, extending out of the culture base, of the rotating shaft of the movable base. According to the invention, the culture dish is mounted on the movable base, and the turnover assembly on the base is driven by the first driving rod on the pipetting module, so that the turnover assembly can drive the movable base to turn over and incline when the pipetting head moves vertically downwards towards the culture dish, and the culture dish is inclined; furthermore, the culture solution in the culture dish can be concentrated at the corner position of the left side of the culture dish, so that the depth of the culture solution is increased, and a transfer pipette can conveniently suck a culture solution sample.

Description

Technical Field

[0001] The present invention relates to the technical field of pipetting, and particularly to a multifunctional pipetting module based on cell culture. Background Art

[0002] A pipetting module is an automated device used for precisely transferring liquid samples. Through a precise robotic arm and pipette tip system, it can achieve accurate aspiration and release of liquids, and is widely used in multiple fields such as chemistry, biology, and medicine to meet different experimental requirements.

[0003] When the existing pipetting module performs a pipetting operation, it will first control the pipette head to move through the robotic arm, align the bottom end of the pipette head with several pipette tubes and lower them, and then sleeve the pipette tubes on the pipette head; then control the pipette tubes to move above the reagent tube or reagent cassette through the robotic arm, and lower them so that the bottom end of the pipette tube is immersed in the liquid reagent; subsequently, suck the liquid reagent into the pipette tube through the pressure control mechanism in the pipetting module, and finally control the pipette tube to move to the position where the liquid needs to be transferred, and inject the liquid reagent into the reagent tube to complete the transfer of the liquid sample.

[0004] However, the above process is only applicable to the transfer of liquids in reagent tubes or reagent cassettes because the depth of the liquid in the reagent tube can immerse the bottom end of the pipette tube. In biological experiments for cell culture, petri dishes are used as containers for carrying liquid reagents, and the depth of the liquid for cell culture in the petri dish is not sufficient to immerse the bottom end of the pipette tube. When it is necessary to aspirate some samples from the petri dish, if the petri dish is always in a horizontal state, it may cause the pipette tube to aspirate insufficient liquid samples, which is not conducive to the staff's accurate observation of cells and affects experimental data. Therefore, we have proposed a multifunctional pipetting module based on cell culture to well solve the above drawbacks. Summary of the Invention

[0005] The purpose of the present invention is to provide a multifunctional pipetting module based on cell culture to solve the problems raised in the above background art.

[0006] The present invention is achieved through the following technical solutions: A multifunctional pipetting module based on cell culture, including a pipetting module, the pipetting module is arranged inside a pipetting workstation, and several pipette heads are provided at the bottom of the pipetting module. It further includes:

[0007] A combined frame, the combined frame includes an outer rectangular frame fixedly arranged at the bottom of the pipetting module, an inner rectangular frame is rotatably arranged on the inner side surface of the outer rectangular frame, and the tops of several pipette heads are all arranged in the inner rectangular frame;

[0008] The culture medium base is arranged on the inner bottom surface of the pipetting workstation. A plurality of installation grooves are formed on the upper surface of the culture medium base. A movable base is rotatably connected in each of the plurality of installation grooves, and the movable base is slidably matched with the installation groove in the horizontal direction. A plurality of culture dishes are placed on the movable base. One end of the rotating shaft on the movable base extends to the outside of the culture medium base and is provided with a first flipping assembly, and the first flipping assembly is used to drive the movable base to rotate;

[0009] The pushing and pulling assembly, the number of the pushing and pulling assemblies is equal to and corresponds to the number of the installation grooves one by one. The pushing and pulling assembly is arranged on the culture medium base, and the pushing and pulling assembly is used to drive the movable base to horizontally slide in the installation groove;

[0010] When the pipetting head points to the left side of the movable base and moves downwards, the first flipping assembly can drive the movable base to tilt and rotate to the left;

[0011] When the pipetting head points to the right side of the movable base and moves downwards, correspondingly the pushing and pulling assembly can drive the movable base to translate to the right.

[0012] Optionally, the first flipping assembly includes a first runner and a first tooth. The first runner is fixedly sleeved on the rotating shaft of the movable base, and the first tooth is fixedly connected to the side surface of the first runner. A second driving rod is fixedly arranged at the bottom of the outer rectangular frame, and the second driving rod is arranged in the vertical direction; when the pipetting head moves to the left half part of the movable base, the second driving rod is directly above the first tooth.

[0013] Optionally, one end of the rotating shaft of the inner rectangular frame connected to the outer rectangular frame extends to the outside of the outer rectangular frame, and a second flipping assembly is fixedly arranged at the end of the rotating shaft of the inner rectangular frame extending to the outside of the outer rectangular frame; the second flipping assembly is used to drive the inner rectangular frame to rotate.

[0014] Optionally, the second flipping assembly and the first flipping assembly have the same shape. The second flipping assembly includes a second runner and a second tooth. The second runner is fixedly sleeved on the rotating shaft of the inner rectangular frame, and the second tooth is fixedly connected to the side surface of the second runner. A plurality of first driving rods are fixedly arranged at the position of the inner side wall of the pipetting workstation close to the culture medium base. The plurality of first driving rods and the plurality of movable bases are arranged in one-to-one correspondence, and the first driving rods are arranged in the height direction of the pipetting workstation; when the pipetting head moves to the right half part of the movable base, the second tooth is directly above the first driving rod.

[0015] Optionally, the push-pull assembly includes two lifting ropes fixedly connected to the side surface of the movable base. The two lifting ropes are symmetrically arranged front and back. A wire passing hole for the lifting rope to pass through is formed in the side wall of the installation groove. One end of each of the two lifting ropes far from the movable base extends to the front and back side surfaces of the culture medium base respectively. A lifting block is fixedly connected to the end of the lifting rope extending outside the culture medium base. The lifting block is slidably arranged on the outer side surface of the culture medium base along the height direction of the culture medium base.

[0016] Optionally, two third driving rods symmetrically arranged front and back are provided at the bottom of the outer rectangular frame. The third driving rods are arranged vertically. When the pipette head moves to the right half of the movable base, the third driving rods are located directly above the lifting blocks.

[0017] Optionally, a return spring is sleeved outside a part of the lifting rope located in the installation groove. The left and right ends of the return spring are respectively abutted against the side surface of the movable base and the inner side wall of the installation groove. In the natural state, the return spring is in a compressed state.

[0018] Optionally, a sliding groove is formed in the side wall of the culture medium base along the width direction of the culture medium base. A sliding sleeve is slidably arranged in the sliding groove along the width direction of the culture medium base. The rotating shaft on the movable base penetrates through the sliding sleeve, and the rotating shaft on the movable base is rotatably connected to the sliding sleeve.

[0019] Optionally, one end of the rotating shaft on the movable base extending outside the culture medium base is rotatably connected to a sliding block. The sliding block is slidably connected to the inner side wall of the pipetting workstation along the length direction of the culture medium base. A torsion spring is sleeved outside the rotating shaft located outside the culture medium base. The two ends of the torsion spring are respectively fixedly connected to the side wall of the sliding block and the side wall of the first runner. When the torsion spring is not subjected to external force, the upper surface of the movable base always remains horizontal.

[0020] Optionally, several pipette heads are respectively slidably connected to the inside of the inner rectangular frame through several moving blocks, and the distance between adjacent two moving blocks is always equal.

[0021] Compared with the prior art, the present invention provides a multifunctional pipetting module based on cell culture, having the following beneficial effects:

[0022] 1. In the present invention, by installing the culture dish on the movable base and using the first driving rod on the pipetting module to drive the flipping component on the base, the flipping component can drive the movable base to flip and tilt when the pipetting head moves vertically downward towards the culture dish, so that the culture dish tilts, and then the culture solution in the culture dish can be concentrated at the corner position on the left side of the culture dish, increasing the depth of the culture solution, facilitating the pipette to aspirate the culture solution sample, and avoiding the influence on experimental data caused by the inability to aspirate the sample.

[0023] 2. When the pipetting head moves to the right side of the culture dish and descends, the inner rectangular frame can drive the pipetting head to flip and tilt. And during the descending process, the culture dish moves to the right side driven by the movable base. Finally, the pipetting orifice of the pipette will be inclined and abutted against the inner wall on the left side of the culture dish, so that when the reagent is injected into the culture dish by the pipette, the cells in the culture dish will not be damaged due to the too fast dripping speed of the reagent liquid, improving the accuracy of experimental data. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 is a schematic side structure diagram of the present invention;

[0025] Figure 2 is a side sectional view of the present invention;

[0026] Figure 3 is a schematic front structure diagram of the present invention;

[0027] Figure 4 is a front sectional view of the culture medium base of the present invention;

[0028] Figure 5 is a side sectional view of the culture medium base of the present invention;

[0029] Figure 6 is a top sectional view of the culture medium base of the present invention;

[0030] Figure 7 is a schematic structure diagram of the pipette in the liquid aspiration state of the present invention;

[0031] Figure 8 is a schematic structure diagram of the pipette in the liquid injection state of the present invention;

[0032] Figure 9 is a schematic diagram of the state where the second flipping component abuts against the first driving rod of the present invention;

[0033] Figure 10 is Figure 2 an enlarged schematic structure diagram at A in

[0034] In the figure: 1. Pipetting module; 101. Pipetting head; 2. Pipetting workstation; 201. First driving rod;; 3. Combined frame; 301. Outer rectangular frame; 302. Inner rectangular frame; 303. Second driving rod; 304. Third driving rod; 4. Culture medium base; 401. Installation groove; 402. Movable base; 403. Slide groove; 404. Slide sleeve; 405. Slide block; 5. First flipping assembly; 501. First runner; 502. First tooth; 6. Pushing and pulling assembly; 601. Lifting rope; 602. Lifting block; 603. Return spring; 7. Second flipping assembly; 701. Second runner; 702. Second tooth; 8. Moving block. Detailed implementation mode

[0035] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0036] Please refer to Figure 1 - Figure 10 , a multifunctional pipetting module based on cell culture, including a pipetting module 1, the pipetting module 1 is arranged inside the pipetting workstation 2, and a plurality of pipetting heads 101 are arranged at the bottom of the pipetting module 1. It also includes a combined frame 3. The combined frame 3 includes an outer rectangular frame 301 fixedly arranged at the bottom of the pipetting module 1. An inner rectangular frame 302 is rotatably arranged on the inner side surface of the outer rectangular frame 301. The tops of a plurality of pipetting heads 101 are all arranged in the inner rectangular frame 302, so that when the inner rectangular frame 302 is flipped inside the outer rectangular frame 301, it can drive the pipetting heads 101 to flip synchronously, thereby changing the inclination angle of the pipetting heads 101, and further changing the inclination angle of the bottom pipetting tube.

[0037] A culture medium base 4 is arranged on the right side of the inner bottom surface of the pipetting workstation 2. A plurality of installation grooves 401 are opened on the upper surface of the culture medium base 4. A movable base 402 is rotatably connected in each of the plurality of installation grooves 401. A plurality of culture dishes are placed on the movable base 402, so that the rotation of the movable base 402 can drive the culture dishes to rotate together, thereby changing the inclination angle of the culture dishes. The movable base 402 and the installation groove 401 are slidably matched in the horizontal direction, so that the movable base 402 can drive the culture dishes to move horizontally together. One end of the rotating shaft on the movable base 402 extends outside the culture medium base 4 and is provided with a first flipping assembly 5, and the first flipping assembly 5 is used to drive the movable base 402 to rotate.

[0038] The following is a detailed description of the first flipping assembly 5 to make the rotation process of the movable base 402 clearer:

[0039] The first flipping component 5 includes a first runner 501 and a first tooth 502. The first runner 501 is fixedly sleeved on the rotating shaft of the movable base 402, and the first tooth 502 is fixedly connected to the side surface of the first runner 501. A second driving rod 303 is fixedly provided at the bottom of the outer rectangular frame 301, and the second driving rod 303 is arranged in the vertical direction. When the pipetting head 101 moves to the left half of the movable base 402, the second driving rod 303 is directly above the first tooth 502, so that when the pipetting head 101 moves above the left half of the movable base 402, the second driving rod 303 can be directly opposite to the first tooth 502. When the pipetting module 1 controls the overall downward movement of the combined frame 3, the outer rectangular frame 301 will drive the second driving rod 303 to move towards the first tooth 502. Until the bottom end of the second driving rod 303 contacts the upper surface of the first tooth 502, the second driving rod 303 will drive the first tooth 502 to rotate around the central axis of the first runner 501, so that the first tooth 502 drives the first runner 501 to rotate. The first runner 501 is fixedly sleeved on the rotating shaft of the movable base 402. Therefore, the movable base 402 will also flip synchronously, so that the left side of the movable base 402 tilts downward. Finally, the bottom end of the pipette will be immersed in the culture medium on the left side of the movable base 402, which is convenient for sucking the culture medium sample and is conducive to the staff's observation and analysis of the culture medium.

[0040] A plurality of pushing and pulling components 6 are further provided on the culture medium base 4. The number of the pushing and pulling components 6 is equal to and corresponds to the number of the installation grooves 401 one by one. The pushing and pulling components 6 are arranged on the culture medium base 4, and the pushing and pulling components 6 are used to drive the movable base 402 to horizontally slide in the installation groove 401, so that the side wall of the culture dish can abut against the bottom end of the pipette.

[0041] Specifically, when the pipetting head 101 points to the left side of the movable base 402 and moves downward, the first flipping component 5 can drive the movable base 402 to tilt and rotate to the left;

[0042] When the pipetting head 101 points to the right side of the movable base 402 and moves downward, the corresponding pushing and pulling component 6 can drive the movable base 402 to translate to the right.

[0043] Further, one end of the rotating shaft of the inner rectangular frame 302 connected to the outer rectangular frame 301 extends out of the outer rectangular frame 301, and a second flipping component 7 is fixedly provided at the end of the rotating shaft of the inner rectangular frame 302 extending out of the outer rectangular frame 301. The second flipping component 7 is used to drive the inner rectangular frame 302 to rotate. The following is a detailed description of the second flipping component 7 to make the rotation process of the inner rectangular frame 302 clearer:

[0044] As Figure 9As shown in the figure, the second flipping component 7 and the first flipping component 5 have the same shape. The second flipping component 7 includes a second runner 701 and second teeth 702. The second runner 701 is fixedly sleeved on the rotating shaft of the inner rectangular frame 302, and the second teeth 702 are fixedly connected to the side surface of the second runner 701. A plurality of first driving rods 201 are fixedly provided at a position on the inner side wall of the pipetting workstation 2 close to the culture medium base 4. The plurality of first driving rods 201 and the plurality of movable bases 402 are arranged in one-to-one correspondence, and the first driving rods 201 are arranged along the height direction of the pipetting workstation 2.

[0045] When the pipetting head 101 moves to the right half of the movable base 402, the second teeth 702 are located directly above the first driving rod 201, so that when the pipetting head 101 moves to the right half of the culture dish on the movable base 402, the second flipping component 7 can follow the outer rectangular frame 301 and descend together until the lower surface of the second teeth 702 contacts the upper surface of the first driving rod 201. Then, as the outer rectangular frame 301 continues to descend, the first driving rod 201 will drive the second teeth 702 to rotate around the central axis of the second runner 701, thereby driving the second runner 701 to flip synchronously. The second runner 701 will drive the inner rectangular frame 302 to rotate through the rotating shaft on the outside of the inner rectangular frame 302, and further change the inclination angle of the pipetting head 101 on the inner rectangular frame 302, and finally tilt the pipetting tube on the pipetting head 101, avoiding the liquid dripping too fast when the pipetting tube injects the reagent into the culture dish, resulting in damage to the cells in the culture dish and affecting the accuracy of the experimental data.

[0046] The following is a detailed description of the push-pull component 6, so that during the process of the pipetting tube moving obliquely downward, the movable base 402 can move towards the direction of the pipetting tube:

[0047] The push-pull component 6 includes two lifting ropes 601 fixedly connected to the side surface of the movable base 402. The two lifting ropes 601 are symmetrically arranged front and back. A wire passing hole for the lifting ropes 601 to pass through is opened on the side wall of the installation groove 401, and the ends of the two lifting ropes 601 far from the movable base 402 respectively extend to the front and rear side surfaces of the culture medium base 4. One end of the lifting rope 601 extending to the outside of the culture medium base 4 is fixedly connected with a lifting block 602. The lifting block 602 is slidably arranged along the height direction of the culture medium base 4 on the outer side surface of the culture medium base 4, so that when the lifting block 602 moves up and down on the outer side surface of the culture medium base 4, it can pull the other end of the movable base 402 to move in the installation groove 401 through the lifting rope 601, thereby changing the position of the movable base 402.

[0048] At the bottom of the outer rectangular frame 301, there are two third driving rods 304 that are symmetrically arranged front and back. The third driving rods 304 are arranged in the vertical direction, and the third driving rods 304 and the second driving rods 303 are staggeredly distributed; so that the driving objects of the third driving rods 304 and the second driving rods 303 will not obstruct each other. When the pipetting head 101 moves to the right half of the movable base 402, the third driving rod 304 is directly above the lifting block 602, that is, when the second tooth 702 is directly above the first driving rod 201, the third driving rod 304 will be directly above the lifting block 602. Thus, during the downward movement of the combined frame 3, the second tooth 702 will drive the pipette to tilt and change the angle of the injected reagent. At the same time, the bottom end of the third driving rod 304 will contact the upper surface of the lifting block 602, so that the third driving rod 304 can push the lifting block 602 downward to slide on the outer side wall of the culture medium base 4. The lifting block 602 will pull the movable base 402 through the lifting rope 601, causing the movable base 402 to move in the installation groove 401, and then driving the culture dish on the movable base 402 to move. Finally, when the bottom end of the pipette enters a certain depth into the culture dish, the left side wall of the culture dish on the movable base 402 will abut against the bottom end of the pipette, so that the liquid can slowly flow down along the side wall of the movable base 402 when the pipette injects the reagent, avoiding damage to the cells.

[0049] In order to enable the culture dish to return to the initial position after the reagent injection is completed, in this application, a return spring 603 is sleeved on the outer side of the part of the lifting rope 601 located in the installation groove 401. The left and right ends of the return spring 603 are respectively in contact with the side surface of the movable base 402 and the inner side wall of the installation groove 401; in the natural state, the return spring 603 is in a compressed state, so that the movable base 402 can return to the initial position after the reagent injection is completed, facilitating subsequent liquid extraction or re-injection operations; and, the return spring 603 is in a contact state with the side surface of the movable base 402 and the inner side wall of the installation groove 401, so that the return spring 603 will not cause obstruction when the movable base 402 is flipped.

[0050] Furthermore, a sliding groove 403 is formed on the side wall of the culture medium base 4 along the width direction of the culture medium base 4. A sliding sleeve 404 is slidably arranged in the sliding groove 403 along the width direction of the culture medium base 4. The rotating shaft on the movable base 402 passes through the sliding sleeve 404, and the rotating shaft on the movable base 402 is rotatably connected to the sliding sleeve 404, so that the movable base 402 can not only be flipped and tilted in the installation groove 401, but also will not affect the left and right movement of the movable base 402 in the installation groove 401.

[0051] It should be noted that one end of the rotating shaft on the movable base 402 extending outside the culture medium base 4 is rotatably connected to a slider 405. The slider 405 is slidably connected to the inner side wall of the pipetting workstation 2 along the length direction of the culture medium base 4. A torsion spring is sleeved outside the rotating shaft located outside the culture medium base 4. Two ends of the torsion spring are respectively fixedly connected to the side wall of the slider 405 and the side wall of the first runner 501. When the torsion spring is not affected by external forces, the upper surface of the movable base 402 always remains horizontal, so that the liquid in the petri dish will not sway left and right, avoiding affecting the state of the cells in the petri dish.

[0052] In this embodiment, several pipette tips 101 are respectively slidably connected inside the inner rectangular frame 302 through several moving blocks 8, and the distance between adjacent two moving blocks 8 is always equal, so that the moving blocks 8 can keep the distance between adjacent two equal while moving, so that the moving blocks 8 can finally correspond to the petri dishes one by one, and further the pipette tips 101 correspond to the petri dishes one by one.

[0053] Specifically, several driving mechanisms corresponding to several moving blocks 8 are arranged inside the inner rectangular frame 302. The driving mechanisms are intelligently controlled by a control system. The several driving mechanisms are respectively used to drive the corresponding moving blocks 8 to move inside the inner rectangular frame 302. For example, the way of using a slide table to control the movement of a slide seat can be used to control the moving blocks 8, so that the several moving blocks 8 approach or move away from each other, and further the pipette tips 101 and the pipette can approach or move away from each other. Finally, the pipette can be used for the pipetting operation of the reagent tube and also for the pipetting operation of the petri dish with a width greater than that of the reagent tube.

[0054] The working principle and usage process of the present invention: First, when performing a liquid suction operation on the petri dish, control the pipette tip 101 to move above the left half of the corresponding petri dish; at this time, the second driving rod 303 will move directly above the first tooth 502. As the pipette tip 101 drives the pipette to descend, the pipette will move into the petri dish. During this process, the bottom end of the second driving rod 303 will contact the upper surface of the first tooth 502, so that the first tooth 502 is driven by the second driving rod 303 to rotate around the central axis of the first runner 501. The first tooth 502 drives the first runner 501 to rotate, the first runner 501 drives the movable base 402 to rotate, and the movable base 402 drives the petri dish to tilt; finally, the culture solution in the petri dish is concentrated on the left side of the petri dish, increasing the liquid depth, and the bottom end of the pipette will also enter the petri dish and be submerged by the culture solution. At this time, the liquid suction operation can be performed.

[0055] When performing the liquid injection operation on the culture dish, control the pipetting head 101 to move above the right half of the corresponding culture dish; at this time, the second tooth 702 will move directly above the first drive rod 201, and the third drive rod 304 will move directly above the lifting block 602. As the pipetting head 101 drives the pipette to descend, the lower surface of the second tooth 702 will contact the upper surface of the first drive rod 201. Subsequently, the second tooth 702 is almost driven by the first drive rod 201 to rotate around the central axis of the second runner 701. The second tooth 702 drives the second runner 701 to rotate, the second runner 701 drives the inner rectangular frame 302 to rotate, the inner rectangular frame 302 drives the pipetting head 101 to rotate, and the pipetting head 101 drives the pipette to change the tilt angle; at this time, the pipette continues to descend and always remains tilted. Subsequently, the third drive rod 304 will contact the lifting block 602, thereby pushing the lifting block 602 downward. The lifting block 602 pulls the movable base 402 to move in the installation groove 401 through the lifting rope 601, and the movable base 402 drives the culture dish to move synchronously to the right; finally, the pipette remains tilted and enters the culture dish. After the left inner wall of the culture dish moves to a state where it abuts against the bottom end of the pipette, the liquid injection operation can be started, and the reagent solution slowly flows down along the inner wall of the culture dish.

[0056] Although the embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that various changes, modifications, substitutions and variations can be made in these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A multifunctional pipetting module based on cell culture, comprising a pipetting module, the pipetting module is arranged inside a pipetting workstation, and a plurality of pipette tips are arranged at the bottom of the pipetting module, characterized in that, Further comprising: A combined frame, the combined frame includes an outer rectangular frame fixedly arranged at the bottom of the pipetting module, an inner rectangular frame is rotatably arranged on the inner side surface of the outer rectangular frame, and the tops of a plurality of the pipetting heads are arranged in the inner rectangular frame; A culture medium base, arranged on the inner bottom surface of the pipetting workstation, a plurality of mounting grooves are formed on the upper surface of the culture medium base, a movable base is rotatably connected in each of the plurality of mounting grooves, and the movable base is slidably matched with the mounting groove in the horizontal direction. A plurality of culture dishes are placed on the movable base. One end of the rotating shaft of the movable base extends outside the culture medium base and is provided with a first flipping assembly, and the first flipping assembly is used to drive the movable base to rotate; A pushing and pulling assembly, the number of the pushing and pulling assemblies is equal to and corresponds to the number of the mounting grooves one by one, the pushing and pulling assembly is arranged on the culture medium base, and the pushing and pulling assembly is used to drive the movable base to horizontally slide in the mounting groove; When the pipetting head points to the left side of the movable base and moves downward, the first flipping assembly can drive the movable base to tilt and rotate to the left; When the pipetting head points to the right side of the movable base and moves downward, correspondingly, the pushing and pulling assembly can drive the movable base to translate to the right.

2. The multifunctional pipetting module based on cell culture according to claim 1, wherein: The first flipping assembly includes a first runner and a first tooth, the first runner is fixedly sleeved on the rotating shaft of the movable base, the first tooth is fixedly connected to the side surface of the first runner, and a second driving rod is fixedly arranged at the bottom of the outer rectangular frame, and the second driving rod is arranged in the vertical direction; when the pipetting head moves to the left half of the movable base, the second driving rod is directly above the first tooth.

3. The multifunctional pipetting module based on cell culture according to claim 2, characterized in that: One end of the rotating shaft of the inner rectangular frame connected to the outer rectangular frame extends outside the outer rectangular frame, and a second flipping assembly is fixedly arranged at the end of the rotating shaft of the inner rectangular frame extending outside the outer rectangular frame; the second flipping assembly is used to drive the inner rectangular frame to rotate.

4. A multifunctional pipetting module based on cell culture according to claim 3, characterized in that: The second flipping assembly and the first flipping assembly have the same shape. The second flipping assembly includes a second runner and a second tooth, the second runner is fixedly sleeved on the rotating shaft of the inner rectangular frame, the second tooth is fixedly connected to the side surface of the second runner, and a plurality of first driving rods are fixedly arranged on the inner side wall of the pipetting workstation near the culture medium base, and the plurality of first driving rods and the plurality of movable bases are arranged in one-to-one correspondence, and the first driving rods are arranged in the height direction of the pipetting workstation; when the pipetting head moves to the right half of the movable base, the second tooth is directly above the first driving rod.

5. A multifunctional pipetting module based on cell culture according to claim 1, characterized in that: The pushing and pulling assembly includes two lifting ropes fixedly connected to the side surface of the movable base, the two lifting ropes are symmetrically arranged front and back, a wire passing hole for the lifting rope to pass through is formed on the side wall of the mounting groove, and the ends of the two lifting ropes far away from the movable base respectively extend to the front and rear side surfaces of the culture medium base, and a lifting block is fixedly connected to the end of the lifting rope extending outside the culture medium base, and the lifting block is slidably arranged on the outer side surface of the culture medium base in the height direction of the culture medium base.

6. The multifunctional pipetting module based on cell culture according to claim 5, wherein: There are two third driving rods symmetrically arranged front and back at the bottom of the outer rectangular frame, and the third driving rods are arranged in the vertical direction; when the pipetting head moves to the right half of the movable base, the third driving rods are located directly above the lifting block.

7. A multifunctional pipetting module based on cell culture according to claim 6, characterized in that: A return spring is sleeved on the outer side of the part of the lifting rope located in the installation groove, and the left and right ends of the return spring are respectively abutted against the side wall of the movable base and the inner side wall of the installation groove; in the natural state, the return spring is in a compressed state.

8. A multifunctional pipetting module based on cell culture according to claim 1, characterized in that: A chute is provided on the side wall of the culture medium base along the width direction of the culture medium base, and a sliding sleeve is slidably arranged in the chute along the width direction of the culture medium base. The rotating shaft on the movable base penetrates through the sliding sleeve, and the rotating shaft on the movable base is rotatably connected to the sliding sleeve.

9. The multifunctional pipetting module based on cell culture according to claim 2, characterized in that: One end of the rotating shaft on the movable base extending out of the culture medium base is rotatably connected with a sliding block. The sliding block is slidably connected to the inner side wall of the pipetting workstation along the length direction of the culture medium base. A torsion spring is sleeved on the outer side of the rotating shaft located outside the culture medium base. The two ends of the torsion spring are respectively fixedly connected to the side wall of the sliding block and the side wall of the first runner; when the torsion spring is not affected by external forces, the upper surface of the movable base always remains horizontal.

10. A multifunctional pipetting module based on cell culture according to claim 1, characterized in that: A plurality of the pipetting heads are respectively slidably connected to the inside of the inner rectangular frame through a plurality of moving blocks, and the distance between adjacent two moving blocks is always equal.

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