Dendrobium officinale-containing composition for promoting synthesis of type I collagen

Abstract

The invention discloses a composition containing dendrobium officinale and capable of promoting synthesis of type I collagen, and belongs to the field of dressing preparations. The composition comprises the following raw materials: a dendrobium officinale extract, an emblic leafflower fruit extract, a mirabilis jalapa extract and ascorbic acid polypeptide, and the raw materials are synergistically compounded, so that the composition provided by the invention has a remarkable effect of promoting synthesis of type I collagen.

Description

Technical Field

[0001] The present invention belongs to the field of toilet preparations, and particularly relates to a composition containing Dendrobium officinale and promoting the synthesis of type I collagen. Background Art

[0002] With the changes in modern lifestyle and the aggravation of environmental pollution, the skin is facing more and more challenges. Among them, skin aging is a common concern. Skin aging is mainly manifested as decreased skin elasticity, increased wrinkles, dull color, etc., and these problems are closely related to the loss of collagen in the skin. Among the collagens in the skin, type I collagen is the main component, which provides elasticity and structural support for the skin, making the skin plump and full. With the influence of environmental factors such as aging, ultraviolet radiation, and free radical attack, type I collagen in the skin will gradually decrease and denature, resulting in the above-mentioned aging signs of the skin. In addition, the fast-paced life, high pressure, and unbalanced diet of modern people will also affect the metabolism of the skin and the synthesis of collagen. Therefore, providing a composition that can effectively promote the synthesis of type I collagen is of great significance for maintaining the healthy state of the skin and delaying skin aging.

[0003] Type I collagen is divided into recombinant type I collagen and type I collagen. The former obtains collagen with a sequence similar to or identical to that of type I collagen through biotechnology, and the latter is the type I collagen autocrine by its own fibroblasts. In the prior art, most methods for synthesizing recombinant type I collagen are to construct engineering bacteria, and then stimulate the engineering bacteria to secrete recombinant type I collagen directionally, in order to obtain recombinant type I collagen with high purity and high yield. The obtained recombinant type I collagen is formulated into cosmetics and sold on the market. Users apply cosmetics containing the recombinant collagen externally to achieve relevant effects. However, such methods have certain defects. For example, the effect of externally applied collagen is relatively short-lived and needs to be used frequently to maintain the effect; externally applied collagen is easily blocked by the stratum corneum on the skin surface and is difficult to penetrate deep into the dermis layer. Moreover, the skin absorption ability and reaction of different people are different, and the effects vary from person to person. Therefore, it is necessary to provide a composition that can stimulate the synthesis of type I collagen by human own fibroblast skin cells to make up for the above disadvantages. Summary of the Invention

[0004] The present invention provides a composition containing Dendrobium officinale and promoting the synthesis of type I collagen. The composition plays a role in promoting the synthesis of type I collagen through the synergistic compounding of Dendrobium officinale extract, Phyllanthus emblica fruit extract, Mirabilis jalapa extract, and ascorbic acid polypeptide.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] In a first aspect, the present invention provides a composition for promoting the synthesis of type I collagen, the composition comprising the following raw materials: Dendrobium officinale extract, Phyllanthus emblica fruit extract, Mirabilis jalapa extract, ascorbic acid polypeptide; the mass ratio of the Dendrobium officinale extract, Phyllanthus emblica fruit extract, Mirabilis jalapa extract, and ascorbic acid polypeptide is 1:(0.5 - 1.5):(0.25 - 0.75):(0.1 - 0.3).

[0007] Preferably, the mass ratio of the Dendrobium officinale extract, Phyllanthus emblica fruit extract, Mirabilis jalapa extract, and ascorbic acid polypeptide in the composition is 1:(0.5 - 1):(0.55 - 0.75):(0.2 - 0.3).

[0008] Preferably, the mass ratio of the Dendrobium officinale extract, Phyllanthus emblica fruit extract, Mirabilis jalapa extract, and ascorbic acid polypeptide in the composition is 1:1:0.55:0.2.

[0009] In a second aspect, the present invention provides the use of the composition according to the first aspect in the preparation of a skin care product having the efficacy of promoting the synthesis of type I collagen.

[0010] Preferably, the dosage form of the skin care product is any one of a cream, a lotion, and a serum.

[0011] In a third aspect, the present invention provides a serum for promoting the synthesis of type I collagen, the serum comprising the following components by mass percentage:

[0012] The composition according to the first aspect: 1 - 3 wt%;

[0013] Sodium hyaluronate: 0.05 - 0.1 wt%;

[0014] Glycerol: 10 - 15 wt%;

[0015] Disodium EDTA: 0.01 - 0.05 wt%;

[0016] Phenoxyethanol: 0.3 - 0.5 wt%;

[0017] The balance is deionized water.

[0018] In a fourth aspect, the present invention provides a preparation method of the serum according to the third aspect, the specific steps being as follows:

[0019] S1: Mix deionized water with the raw materials in the composition and stir evenly to obtain a mixed solution;

[0020] S2: Add glycerol, sodium hyaluronate, disodium EDTA, and phenoxyethanol to the mixed solution in sequence, and stir and mix evenly to obtain the serum.

[0021] In the present invention:

[0022] Extract of Dendrobium officinale: Dendrobium officinale Kimura et Migo, a plant of the genus Dendrobium in the Orchidaceae family. Its extract is rich in polysaccharides, dendrobine, amino acids, vitamins, etc., and has various effects such as nourishing yin and moistening dryness, enhancing immunity, and antioxidant. At the same time, it can promote the synthesis of type I collagen, help improve the elasticity and firmness of the skin, and delay skin aging.

[0023] Extract of Mirabilis jalapa: Mirabilis jalapa L., a plant of the genus Mirabilis in the Nyctaginaceae family. Its extract is rich in flavonoids, alkaloids, etc., and has effects such as antioxidant and anti-inflammatory. It can regulate the synthesis of collagen in the dermis and also has a certain effect on promoting the synthesis of type I collagen.

[0024] Extract of Phyllanthus emblica fruit: Phyllanthus emblica Linn., a plant of the genus Phyllanthus in the Euphorbiaceae family. Its fruit extract is rich in ellagic acid, ascorbic acid, flavonoids, phenolic compounds, etc., and has effects such as antioxidant, anti-inflammatory, and antibacterial. It can indirectly promote the synthesis of type I collagen by increasing the expression of type XVII collagen (COL17A1) in hair follicles and restricting its degradation.

[0025] Ascorbic acid polypeptide: Ascorbic acid polypeptide is a derivative of vitamin C, formed by combining vitamin C with polypeptides. It has effects such as antioxidant, promoting collagen production, whitening, and improving skin microcirculation. It can effectively promote the synthesis of type I collagen and improve the elasticity and firmness of the skin.

[0026] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0027] The present invention combines and uses the extracts of Dendrobium officinale, Mirabilis jalapa, Phyllanthus emblica fruit, and ascorbic acid polypeptide. Among them, ascorbic acid polypeptide has good permeability, which helps to efficiently transfer the active ingredients in each plant extract to the deep layer of the skin during use, reducing the oxidation and inactivation of the active substances on the skin surface of skin care products, thereby improving the skin care effect; the combination of the extract of Dendrobium officinale with the extracts of Mirabilis jalapa and Phyllanthus emblica fruit can enhance each other's effects in the skin. There is a significant synergistic effect among the raw materials in the composition provided by the present invention. Especially in promoting the synthesis of type I collagen, it can more effectively promote the synthesis of collagen, enhance the elasticity and firmness of the skin, and delay skin aging. Detailed implementation mode

[0028] To better understand the present invention, the present invention will be further described below in conjunction with specific embodiments. The terms used in the embodiments are for describing specific implementation manners and do not constitute a limitation on the protection scope of the present invention.

[0029] For the experimental methods without specific conditions indicated in the following embodiments, they are generally carried out under conventional conditions or according to the conditions recommended by the manufacturers. Unless otherwise stated, percentages and parts are calculated by weight.

[0030] The sources of some raw materials are as follows:

[0031] Dendrobium officinale extract: purchased from Guangzhou Pinyan Biotechnology Co., Ltd.;

[0032] Mirabilis jalapa extract: purchased from Guangzhou Xincheng Meike Biotechnology Co., Ltd.;

[0033] Phyllanthus emblica fruit extract: purchased from Yunnan Yingge Biotechnology Co., Ltd.;

[0034] Ascorbic acid polypeptide: purchased from Hemei Biotechnology Co., Ltd.;

[0035] Other raw materials and reagents are all commercially available.

[0036] A composition for promoting the synthesis of type I collagen, the specific composition and mass ratio of its raw materials are as follows:

[0037] Composition 1: composed of Dendrobium officinale extract, Phyllanthus emblica fruit extract, Mirabilis jalapa extract, and ascorbic acid polypeptide, and the mass ratio is 1:1:0.55:0.2.

[0038] Composition 2: composed of Dendrobium officinale extract, Phyllanthus emblica fruit extract, Mirabilis jalapa extract, and ascorbic acid polypeptide, and the mass ratio is 1:0.5:0.75:0.3.

[0039] Composition 3: composed of Dendrobium officinale extract, Phyllanthus emblica fruit extract, Mirabilis jalapa extract, and ascorbic acid polypeptide, and the mass ratio is 1:1.5:0.25:0.1.

[0040] Composition ①: Different from Composition 1, it lacks Dendrobium officinale extract, and the lacking mass is supplemented with Phyllanthus emblica fruit extract, Mirabilis jalapa extract, and ascorbic acid polypeptide with a mass ratio of 1:0.55:0.2.

[0041] Composition ②: Different from Composition 1, it lacks Phyllanthus emblica fruit extract, and the lacking mass is supplemented with Dendrobium officinale extract, Mirabilis jalapa extract, and ascorbic acid polypeptide with a mass ratio of 1:0.55:0.2.

[0042] Composition ③: Different from Composition 1, it lacks Mirabilis jalapa extract, and the lack of mass is supplemented with Dendrobium officinale extract, Phyllanthus emblica fruit extract, and ascorbic acid polypeptide in a mass ratio of 1:1:0.2.

[0043] Composition ④: Different from Composition 1, it lacks ascorbic acid polypeptide, and the lack of mass is supplemented with Dendrobium officinale extract, Phyllanthus emblica fruit extract, and Mirabilis jalapa extract in a mass ratio of 1:1:0.55.

[0044] Composition ⑤: Different from Composition 1, the mass ratio of Dendrobium officinale extract, Phyllanthus emblica fruit extract, Mirabilis jalapa extract, and ascorbic acid polypeptide is 1:0.4:1:0.4.

[0045] Test for promoting type I collagen synthesis:

[0046] Administration group: Divided into sample group and positive control group. The sample group was prepared by using sterile deionized water to prepare mixed solutions of Compositions 1-3 and Compositions ①-⑤ with a concentration of 0.5 mg / L; the positive control group was prepared by using sterile deionized water to prepare a solution of TGF-β1 with a concentration of 100 ng / mL.

[0047] Test method: Refer to T / SHRH 031-2020 "Cosmetics Firming and Anti-Wrinkle Efficacy Test - Determination of Type I Collagen Content in In Vitro Fibroblasts" to test the effect of the administration group on promoting the synthesis of type I collagen. Three parallel experiments were set for each sample solution in each group, and the experimental results were expressed as mean ± variance. See Table 1 for details.

[0048] Calculation formula for type I collagen upregulation rate:

[0049] Type I collagen upregulation rate / % = [(T / C) - 1] × 100%

[0050] Where T is the average value of type I collagen content in the administration group; C is the average value of type I collagen content in the solvent group (replacing the sample group solution with an equal amount of sterile deionized water).

[0051] Table 1 Type I collagen upregulation rate

[0052] Sample Name Upregulation Rate of Type I Collagen (%) Composition 1 <![CDATA[38.45±0.34 # > Composition 2 <![CDATA[36.54±0.29 # > Composition 3 <![CDATA[37.33±0.29 # > Composition ① <![CDATA[24.56±0.18 a# > Composition ② <![CDATA[26.37±0.28 a# > Composition ③ <![CDATA[18.54±0.06 a# > Composition ④ <![CDATA[20.39±0.11 a# > Composition ⑤ <![CDATA[32.59±0.46 a# > Positive Control Group 46.56±0.14

[0053] Note: "a" indicates p < 0.05 compared with Composition 1; "#" indicates p < 0.05 compared with the positive control group.

[0054] Test results: By comparing the results of Composition 1 with Compositions ①-⑤, it can be seen that there is a significant synergistic effect among Dendrobium officinale extract, Phyllanthus emblica fruit extract, Mirabilis jalapa extract, and ascorbic acid polypeptide in enhancing the type I collagen upregulation rate in the present invention.

[0055] Specifically, by comparing the results of Composition 1 with Compositions ①-④, it can be seen that there is a certain synergistic effect among Dendrobium officinale extract, Phyllanthus emblica fruit extract, Mirabilis jalapa extract, and ascorbic acid polypeptide. The possible mechanism is as follows: Dendrobium officinale extract promotes the proliferation of fibroblasts and the synthesis of collagen by activating signal pathways such as PI3K / Akt and MAPK / ERK; Phyllanthus emblica fruit extract inhibits the activation of the NF-κB signal pathway through antioxidant and anti-inflammatory effects, reducing the negative impact of inflammatory factors on collagen production; Mirabilis jalapa extract may promote the expression of collagen-related genes and the synthesis of proteins by regulating intracellular signal pathways. The three promote the production of type I collagen from different pathways, enhancing the elasticity and firmness of the skin; while ascorbic acid polypeptide has good permeability, which helps to promote the effective components in each plant extract to be better absorbed by the skin during use, reducing the oxidation and inactivation of effective substances on the skin surface. By comparing the results of Composition 1 with Composition ⑤, it can be seen that when the dosage ratio of each raw material in the composition for promoting the synthesis of type I collagen is within the range defined in the present invention, the composition has a good effect on promoting the synthesis of type I collagen.

[0056] An essence for promoting the synthesis of type I collagen, the components and their mass percentages are shown in Table 2 below:

[0057] Table 2 Components and mass percentages of the essence

[0058]

[0059] The specific preparation steps of the essence for promoting the production of type I collagen are as follows:

[0060] S1: Mix deionized water with each raw material in the composition and stir evenly to obtain a mixed solution;

[0061] S2: Add glycerol, sodium hyaluronate, disodium EDTA, and phenoxyethanol to the mixed solution in sequence and stir evenly to obtain the essence.

[0062] Safety test

[0063] Taking the "Technical Specifications for Cosmetic Safety" in 2015 as the reference standard, the cosmetic irritation evaluation of Essences 1-5 and the blank essence was carried out. The test method was the skin patch test, and 60 people randomly distributed aged 16-65 were tested, with 10 people in each group.

[0064] Test method: Place the test substance in a patch tester, with a dosage of 0.020 - 0.025 g. Cover the patch tester with the test substance on the back or the flexor side of the forearm of the subject with a non-irritating cloth tape. Gently press with the palm to make it evenly adhere to the skin surface for 24 hours. 30 minutes after removing the test patch tester, observe the skin reaction after the indentation disappears. If the result is negative, observe again at 24 hours and 48 hours after the patch test respectively.

[0065] Evaluation criteria:

[0066] Grade 0: Negative reaction;

[0067] Grade 1: Suspected reaction, only with faint erythema;

[0068] Grade 2: Weak positive reaction, with erythema, infiltration, edema, and may have papules;

[0069] Grade 3: Strong positive reaction, with erythema, infiltration, edema, may have papules, and the reaction may extend beyond the test area;

[0070] Grade 4: Extremely strong positive reaction, with obvious erythema, severe infiltration, edema, confluent herpes, and the reaction extends beyond the test area.

[0071] Test results: The skin reactions of all subjects were negative.

[0072] The above test results indicate that the essence provided by the present invention is mild and non-irritating to the skin and is safe to use.

[0073] Human efficacy test

[0074] Test samples: Essence 1 - 5, blank essence.

[0075] Volunteer screening: Select 180 healthy female subjects (35 - 55 years old) with dry lines, fine lines, skin relaxation, and reduced skin elasticity on the face. Randomly divide the subjects into 6 groups, with 30 people in each group, and control the average age of each group to be as close as possible.

[0076] Test method: After cleaning the skin of each group of volunteers with warm water, a skin elasticity tester was used to measure the skin elasticity parameter R1 of the subjects' cheek area, and the average value was taken after measuring three times. After the measurement was completed, 3 mL of essence was evenly applied to the face and gently massaged with the fingertips until completely absorbed, once in the morning and once in the evening. During the continuous 8-week test period, no other products of the same type could be used. The test samples were essence 1-5 and blank essence. The volunteers in the same group used the same product. After 8 weeks, the skin elasticity parameter R2 of the same cheek area of the subjects was measured again using a skin elasticity tester, and the average value was taken after measuring three times. R1 and R2 represent the ratio of the skin's rebound amount without negative pressure to the maximum stretching amount of the skin with negative pressure, indicating the degree to which the skin can recover within one cycle after being deformed by negative pressure. The closer the test value is to 1, the better the skin elasticity. See Table 3 for details.

[0077] Skin elasticity improvement rate = (R2 - R1) / R1 × 100%

[0078] Table 3 Skin elasticity test results

[0079] Sample Group Skin Elasticity Enhancement Rate / % Essence 1 32.517 Essence 2 30.624 Essence 3 31.556 Essence 4 24.985 Essence 5 33.924 Blank Essence 0.962

[0080] As can be seen from the results in Table 3, after 8 weeks of use, compared with the blank essence, the skin elasticity improvement rates of essence 1-5 were significantly higher than those of the blank essence, indicating that the composition and essence provided by the present invention have a significant effect on improving skin elasticity, further proving that each plant extract in the product of the present invention can effectively promote the production of collagen, maintain and enhance the elasticity of the skin, and ultimately achieve the result of synergistic anti-aging.

[0081] The embodiments described above are some, but not all, of the embodiments of the present application. The detailed description of the embodiments of the present application is not intended to limit the scope of the present application claimed, but merely represents the selected embodiments of the present application. All other embodiments obtained by those skilled in the art based on the embodiments in the present application without creative efforts fall within the scope of protection of the present application.

Claims

1. A composition for promoting the synthesis of type I collagen, characterized in that, It contains the following raw materials: Dendrobium officinale extract, Phyllanthus emblica fruit extract, Mirabilis jalapa extract, and ascorbic acid polypeptide; the mass ratio of the Dendrobium officinale extract, Phyllanthus emblica fruit extract, Mirabilis jalapa extract, and ascorbic acid polypeptide is 1:(0.5 - 1.5):(0.25 - 0.75):(0.1 - 0.3).

2. The composition according to claim 1, wherein, The mass ratio of the Dendrobium officinale extract, Phyllanthus emblica fruit extract, Mirabilis jalapa extract, and ascorbic acid polypeptide is 1:(0.5 - 1):(0.55 - 0.75):(0.2 - 0.3).

3. The composition according to claim 1, wherein The mass ratio of the Dendrobium officinale extract, Phyllanthus emblica fruit extract, Mirabilis jalapa extract, and ascorbic acid polypeptide is 1:1:0.55:0.

2.

4. Use of a composition according to any one of claims 1 - 3 in the preparation of a skin care product having the effect of promoting type I collagen synthesis.

5. The application according to claim 4, characterized in that, The dosage form of the skin care product is any one of cream, lotion, and essence.

6. An essence for promoting the synthesis of type I collagen, characterized in that, It includes the following components by mass percentage: The composition according to any one of claims 1 - 3: 1 - 3 wt%; Sodium hyaluronate: 0.05 - 0.1 wt%; Glycerol: 10 - 15 wt%; Disodium EDTA: 0.01 - 0.05 wt%; Phenoxyethanol: 0.3 - 0.5 wt%; The balance is deionized water.

7. A method for preparing the essence according to claim 6, characterized in that, It includes the following steps: S1: Mix deionized water with each raw material in the composition and stir evenly to obtain a mixed solution; S2: Add glycerol, sodium hyaluronate, disodium EDTA, and phenoxyethanol to the mixed solution in sequence, stir and mix evenly to obtain the essence.

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