Goat milk extract with anti-wrinkle and repairing effects and enzymolysis biological preparation method

Abstract

The invention discloses a goat milk extract with anti-wrinkle and repairing effects and an enzymolysis biological preparation method, and particularly relates to the technical field of cosmetics, the goat milk extract is prepared by performing low-temperature vacuum de-odoring treatment and treating easily-sensitized protein by adopting plant protease and microbial protease, and the goat milk extract is free of goaty flavor and sensitization, and has no side effects. Nutritional ingredients and effects of goat milk are reserved; the preparation method comprises the steps of goat milk pretreatment, low-temperature vacuum de-odoring, enzymolysis, centrifugal separation and the like. The extract is applied to cosmetic compositions, contains various components such as goat milk extract, other functional skin care product additives, nicotinamide and the like, and can synergistically promote collagen synthesis, enhance epidermis barrier repair capability and realize anti-wrinkle and repair effects.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics. More specifically, the present invention relates to a goat milk extract with anti-wrinkle and repair effects and an enzymatic biological preparation method therefor. Background Art

[0002] With the continuous improvement of consumers' skin care needs, products with anti-wrinkle and repair effects are highly favored. Goat milk is rich in various nutrients such as proteins, vitamins, minerals, etc., and has potential nourishing and repairing effects on the skin. In theory, it is an excellent raw material for cosmetics. However, goat milk has some inherent defects that limit its wide application in cosmetics.

[0003] On the one hand, goat milk has an obvious muttony smell, which will affect consumers' usage experience and reduce the acceptance of products. Traditional deodorization methods often fail to completely remove the muttony smell, and may damage the nutrients in goat milk during the deodorization process, resulting in a significant reduction in its skin care effects. On the other hand, goat milk contains some easily allergenic proteins, such as β-lactoglobulin. Some consumers may have allergic reactions after using cosmetics containing such components, which will not only damage consumers' skin health but also increase the safety risks of products.

[0004] At present, although there are a wide variety of anti-wrinkle and repair cosmetics on the market, most products have limitations in terms of ingredients and effects. Although some products using chemically synthesized ingredients can achieve certain effects in the short term, long-term use may have side effects on the skin. And some products mainly based on natural ingredients have deficiencies in raw material treatment and efficacy synergy, and cannot fully exert the advantages of natural ingredients. For example, some products fail to effectively solve the problems of odor and allergenicity of raw materials, causing many troubles for consumers in the pursuit of beauty and health. Therefore, it is of great market value and practical significance to develop a goat milk extract and related cosmetics that can make full use of the nutrients and skin care effects of goat milk while overcoming its muttony smell and allergenicity problems. Summary of the Invention

[0005] In order to overcome the above-mentioned defects of the prior art, the present invention provides a goat milk extract with anti-wrinkle and repair effects and an enzymatic biological preparation method therefor to solve the problems raised in the above background art.

[0006] To achieve the above object, the present invention provides the following technical solution: An enzymatic biological preparation method of a goat milk extract with anti-wrinkle and repair effects, wherein the goat milk extract is prepared by subjecting goat milk to low-temperature vacuum deodorization treatment and treating the easily allergenic proteins with plant protease and microbial protease. The goat milk extract retains the nutrients and effects of goat milk, and has no muttony smell or allergenicity of goat milk;

[0007] It includes the following steps:

[0008] Pre-treat fresh goat milk and filter to remove impurities;

[0009] Cool the pre-treated goat milk to 0 - 10°C and conduct low-temperature vacuum deodorization treatment under the condition of a vacuum degree of 0.075 - 0.090 MPa for 12 - 24 hours;

[0010] Add plant protease and microbial protease to the deodorized goat milk. The addition amount of plant protease is 0.1% - 0.3% of the weight of goat milk, and the addition amount of microbial protease is 0.2% - 0.5% of the weight of goat milk. Enzymatically hydrolyze at a temperature of 45 - 55°C for 2 - 4 hours;

[0011] After the enzymatic hydrolysis ends, centrifuge the enzymatic hydrolysate and take the supernatant to obtain the goat milk extract.

[0012] Avoid loss of heat-sensitive components through low temperature, accelerate the volatilization of odor substances in a vacuum environment, and retain nutritional components.

[0013] The combined action of plant protease and microbial protease targets and decomposes allergenic proteins, such as β-lactoglobulin contained in goat milk, thereby reducing allergenicity. Among them, plant protease is preferably papain, and microbial protease is preferably subtilisin, achieving thorough deodorization, almost negligible allergenicity, and being able to retain active ingredients.

[0014] Preferably, the plant protease is ficin or bromelain, the microbial protease is thermolysin or neutral protease, and 0.05% - 0.1% of antioxidant is added after enzymatic hydrolysis. The antioxidant includes but is not limited to vitamin E, tea polyphenols and their mixtures.

[0015] Ficin has a high affinity for collagen and can enhance the anti-wrinkle effect; thermolysin is heat-resistant and improves stability, while the addition of antioxidant can inhibit the oxidation of active ingredients during enzymatic hydrolysis and extend the shelf life.

[0016] Application of goat milk extract with anti-wrinkle and repair effects in a cosmetic composition. The cosmetic composition contains the following raw materials in weight percentages: 3% - 10% of goat milk extract; 3% - 5% of other functional skin care additives; 1% - 5% of niacinamide; 0.8% - 1.2% of trehalose; 3% - 12% of glycerol; 1% - 2.5% of glyceryl stearate; 1% - 5% of plant stem cell extract; the balance is water;

[0017] The plant stem cell extract is selected from centella asiatica stem cells, grape stem cells and their mixtures;

[0018] The other functional skin care product additives described above are made from raw materials with the following weight ratios: Aloe Barbadensis Leaf Juice 2.5% - 7.5%; Glycyrrhiza Glabra Root Extract 3% - 7%; Piper Methysticum Leaf / Root / Stem Extract 3.5% - 7.5%; Prunus Armeniaca Kernel Oil 5% - 8%.

[0019] The goat milk extract and niacinamide are used synergistically to promote collagen synthesis and reduce the depth of wrinkles. In combination with the plant stem cell extract, it enhances the epidermal barrier repair ability by reducing the transdermal water loss rate.

[0020] A method for preparing a cosmetic composition containing a goat milk extract with anti-wrinkle and repair effects, comprising the following steps:

[0021] Add glycerol, glyceryl stearate, and plant stem cells to water, heat to 75°C to dissolve, mix evenly, and homogenize for 3 - 5 minutes;

[0022] Cool to 45°C, and add the goat milk extract, other functional skin care product additives, niacinamide, and trehalose under stirring, and stir evenly;

[0023] Cool to 30°C, discharge, stand, fill, and package.

[0024] The present invention has the following beneficial effects:

[0025] In the present invention, the goat milk extract is subjected to low-temperature vacuum deodorization treatment to effectively remove the odor of goat milk, making it more acceptable to consumers; plant protease and microbial protease are used to treat the allergenic proteins, significantly reducing the allergenicity of goat milk and broadening its application scope in the cosmetic field. At the same time, ficin has a high affinity for collagen, which can enhance the anti-wrinkle effect; thermophilic protease is heat-resistant, improving the stability of the extract. An antioxidant is added after enzymatic hydrolysis to inhibit the oxidation of active ingredients and extend the shelf life of the product; the preparation method is simple and feasible, with low cost, easy for industrial production, and has good market application prospects.

[0026] The skin care composition of the present invention contains a goat milk extract, which is rich in various nutrients, can provide comprehensive care for the skin, and has the effects of continuous moisturizing, whitening and freckle removal, and antioxidant;

[0027] Through the synergistic effect of the goat milk extract and niacinamide, it can promote collagen synthesis, effectively reduce the depth of wrinkles, and in combination with the plant stem cell extract, it can reduce the transdermal water loss rate and enhance the epidermal barrier repair ability;

[0028] By adding other functional skin care product additives such as Aloe Barbadensis Leaf Juice and Glycyrrhiza Glabra Root Extract, the whitening, anti-wrinkle and repair effects of the composition are further enhanced;

[0029] The composition of the present invention has low allergenicity and is suitable for people with various skin types. No feedback on allergic reactions has been received after long-term use. Detailed implementation manners

[0030] Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0031] Embodiment 1

[0032] Embodiment 1 provides an enzymatic biological preparation method of goat milk extract with anti-wrinkle and repair effects, including the following steps:

[0033] Take 1000 g of fresh goat milk and filter to remove impurities;

[0034] Cool the goat milk to 0 - 10 °C and perform low-temperature vacuum deodorization treatment under the condition of a vacuum degree of 0.080 MPa for 16 hours;

[0035] Add 1 g of papain and 2 g of subtilisin to the deodorized goat milk and enzymolyze at a temperature of 50 °C for 3 hours;

[0036] After the enzymatic hydrolysis is completed, centrifuge the enzymatic hydrolysate and take the supernatant to obtain goat milk extract.

[0037] Embodiment 2

[0038] Embodiment 2 provides an enzymatic biological preparation method of goat milk extract with anti-wrinkle and repair effects, including the following steps:

[0039] Take 2000 g of fresh goat milk and filter to remove impurities;

[0040] Cool the goat milk to 0 - 10 °C and perform low-temperature vacuum deodorization treatment under the condition of a vacuum degree of 0.085 MPa for 2 hours;

[0041] Add 2 g of ficin and 3 g of thermolysin to the deodorized goat milk and enzymolyze at a temperature of 52 °C for 4 hours;

[0042] After the enzymatic hydrolysis is completed, centrifuge the enzymatic hydrolysate and take the supernatant to obtain goat milk extract.

[0043] Embodiment 3

[0044] Embodiment 3 provides a preparation method of a cosmetic composition of goat milk extract with anti-wrinkle and repair effects, including the following steps:

[0045] Take 100 g of glycerol, 20 g of glyceryl stearate, 15 g of centella asiatica stem cells, grape stem cells, add them to water, heat to 75 °C for dissolution, mix evenly, and homogenize for 3 minutes;

[0046] Cool to 45 °C, add 80 g of goat milk extract, 25 g of aloe barbadensis leaf juice, 30 g of glycyrrhiza glabra root extract, 35 g of piper methysticum leaf / root / stem extract, 50 g of argania spinosa kernel oil, 35 g of niacinamide and 8 g of trehalose under stirring, and stir evenly;

[0047] Cool to 30 °C, discharge, stand, fill, and package.

[0048] Example 4

[0049] This Example 4 provides a preparation method of a cosmetic composition with anti-wrinkle and repair effects containing goat milk extract, including the following steps:

[0050] Take 120 g of glycerol, 25 g of glyceryl stearate, 30 g of centella asiatica stem cells, grape stem cells, add them to water, heat to 75 °C for dissolution, mix evenly, and homogenize for 5 minutes;

[0051] Cool to 45 °C, add 100 g of goat milk extract, 30 g of aloe barbadensis leaf juice, 40 g of glycyrrhiza glabra root extract, 50 g of piper methysticum leaf / root / stem extract, 70 g of argania spinosa kernel oil, 40 g of niacinamide and 10 g of trehalose under stirring, and stir evenly;

[0052] Cool to 30 °C, discharge, stand, fill, and package.

[0053] The above are only the preferred embodiments of the present invention and are not used to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. Enzymatic biological preparation method of goat milk extract with anti-wrinkle and repair effects, characterized in that, The goat milk extract is prepared by subjecting goat milk to low-temperature vacuum deodorization treatment and treating the allergenic proteins with plant protease and microbial protease. The goat milk extract retains the nutritional components and efficacy of goat milk, and has no goat milk odor and allergenicity; It includes the following steps: Pre-treat the fresh goat milk and filter to remove impurities; Cool the pre-treated goat milk to 0-10°C and perform low-temperature vacuum deodorization treatment under the condition of a vacuum degree of 0.075-0.090 MPa, and the deodorization time is 12-24 hours; Add plant protease and microbial protease to the deodorized goat milk. The addition amount of plant protease is 0.1%-0.3% of the weight of goat milk, and the addition amount of microbial protease is 0.2%-0.5% of the weight of goat milk, and enzymatically hydrolyze at a temperature of 45-55°C for 2-4 hours; After the enzymatic hydrolysis is completed, centrifuge the enzymatic hydrolysate and take the supernatant to obtain the goat milk extract.

2. The enzymatic biological preparation method of goat milk extract with anti-wrinkle and repair effects according to claim 1, characterized in that, The plant protease is ficin or bromelain; the microbial protease is thermophilic protease or neutral protease; and 0.05%-0.1% of antioxidant is added after enzymatic hydrolysis, and the antioxidant includes but is not limited to vitamin E, tea polyphenols and their mixtures.

3. Use of the goat milk extract having anti-wrinkle and repair effects according to any one of claims 1 or 2 in a cosmetic composition, characterized in that, The cosmetic composition contains the following raw materials in weight percentages: 3%-10% of goat milk extract; 3%-5% of other functional skin care product additives; 1%-5% of niacinamide; 0.8%-1.2% of trehalose; 3%-12% of glycerol; 1%-2.5% of glyceryl stearate; 1%-5% of plant stem cell extract; the balance is water; Among them, the other functional skin care product additives are made of raw materials with the following weight ratios: 2.5%-7.5% of aloe barbadensis leaf juice; 3%-7% of glabra root extract; 3.5%-7.5% of piper methysticum leaf / root / stem extract; 5%-8% of argan kernel oil.

4. Use of the goat milk extract having anti-wrinkle and repair effects according to claim 3 in a cosmetic composition, characterized in that, The plant stem cell extract is selected from centella asiatica stem cells, grape stem cells and their mixtures.

5. A method for preparing a cosmetic composition of goat milk extract with anti-wrinkle and repair effects according to any one of claims 3 or 4, characterized in that, It includes the following steps: Add glycerol, glyceryl stearate and plant stem cells to water, heat to 75°C to dissolve, mix evenly, and homogenize for 3-5 minutes; Cool to 45°C, add goat milk extract, other functional skin care product additives, niacinamide and trehalose under stirring, and stir evenly; Cool to 30°C, discharge, stand, fill and package.