Pore cleaning composition containing medicinal phellinus igniarius as well as preparation method and application of pore cleaning composition

Abstract

The invention discloses a pore cleaning composition containing medicinal phellinus igniarius as well as a preparation method and application of the pore cleaning composition, and belongs to the technical field of cosmetics. The pore cleaning composition provided by the invention is prepared from the following components in parts by mass: 0.1 to 10 parts of a two-way fermentation product, 0.1 to 5 parts of a guaiacol extract, 0.01 to 1 part of madecassoside, 0.01 to 3 parts of a coffee seed extract and 0.01 to 2 parts of sophocarpidine, the bidirectional fermentation product is a medicinal phellinus igniarius-lactobacillus plantarum bidirectional fermentation product. According to the invention, by selecting the components in proper parts by mass, the components interact with one another and cooperate with one another, so that multi-channel synergism of sebum oxidation inhibition, cutin accumulation prevention, anti-inflammation, sterilization and the like can be realized, and the effects of rapidly reducing blackheads and white heads and shrinking pores in a long-acting manner are realized; meanwhile, the pore cleaning composition provided by the invention is mild and non-irritant, and is especially suitable for people with sensitive skin.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and particularly relates to a pore-cleansing composition containing Phellinus officinalis, and a preparation method and application thereof. Background Art

[0002] The formation of blackheads and whiteheads is closely related to sebum hypersecretion, abnormal follicular keratinization, and microbial proliferation. The sebum over-secreted by sebaceous glands accumulates in hair follicles and combines with the excessive proliferation of keratinocytes to form comedones. The main difference between blackheads (open comedones) and whiteheads (closed comedones) lies in the exposure of comedones. Blackheads are caused by the oxidation of sebum exposed to air, which turns black, while whiteheads are caused by the blockage of hair follicle outlets, resulting in the accumulation of sebum. The overgrowth of Propionibacterium acnes also promotes the formation of blackheads and causes skin inflammation. The long-term accumulation of sebum will stretch the hair follicles, resulting in enlarged pores. In addition, repeated inflammatory reactions will further damage the tissue structure around hair follicles, leading to pore dilation.

[0003] Existing methods for removing blackheads and whiteheads are diverse, but each has its limitations. Chemical exfoliation (such as salicylic acid, fruit acids, etc.) can effectively dredge pores, but it is highly irritating to the skin, and long-term use may cause damage to the skin barrier. Physical cleansing methods, such as adsorption mud masks and comedone extractors, can clean pores in the short term, but the effect is not lasting and it is easy to cause skin damage and even aggravate inflammation. Keratin metabolism-regulating components such as retinol and phytoretinols can improve the normal metabolism of the stratum corneum, and long-term use helps to reduce the formation of comedones, but their effects appear slowly and they are somewhat irritating, and may cause discomfort on sensitive skin. Therefore, there are still certain deficiencies in the comprehensiveness, long-term effectiveness, rapidity, and safety of the existing technologies. Summary of the Invention

[0004] The purpose of the present invention is to overcome the deficiencies of the above-mentioned existing technologies, and provide a pore-cleansing composition that can inhibit sebum oxidation, prevent keratin accumulation, and have multi-path synergy such as anti-inflammatory and bactericidal effects, thereby being able to effectively, rapidly, gently, and long-lastingly reduce blackheads and whiteheads and shrink pores, as well as a preparation method and application thereof.

[0005] To achieve the above purpose, in the first aspect of the present invention, the present invention provides a pore-cleansing composition containing Phellinus officinalis, and the pore-cleansing composition includes the following components in parts by mass:

[0006] Bidirectional fermentation product 0.1 - 10 parts, guaiac extract 0.1 - 5 parts, asiaticoside 0.01 - 1 part, small fruit coffee seed extract 0.01 - 3 parts, matrine 0.01 - 2 parts.

[0007] By selecting appropriate components in terms of parts by mass and allowing the components to interact with and cooperate with each other, the present invention can achieve synergy through multiple pathways such as inhibiting sebum oxidation, preventing keratin accumulation, anti-inflammation, and sterilization, thereby rapidly and long-lastingly reducing blackheads and whiteheads and achieving the effect of pore shrinking. At the same time, the pore cleansing composition provided by the present invention is mild and non-irritating, and is particularly suitable for people with sensitive skin.

[0008] Specifically, the inventors speculate that each component has the following effects: The two-way fermentation product Phellinus linteus is rich in triterpenoids and polysaccharides, which can effectively regulate sebum secretion, inhibit the activity of 5α-reductase, reduce excessive oil, and at the same time prevent sebum oxidation through antioxidant effects and reduce the formation of blackheads. In addition, by selecting Phellinus linteus and Lactobacillus plantarum for two-way fermentation, Phellinus linteus can produce active substances that were not present before fermentation under the action of Lactobacillus plantarum, thus better achieving the effects of the present invention. The guaiac extract contains guaiaconic acid and has a significant anti-inflammatory and soothing effect, which can quickly relieve inflammation around the hair follicles and effectively inhibit the growth of Propionibacterium acnes, reducing horn plug infections caused by bacteria. The asiaticoside activates the PPAR-α receptor to promote ceramide synthesis, thereby repairing the damaged skin barrier, and reduces skin inflammatory responses by inhibiting the NF-κB pathway, preventing inflammatory pore problems. The Coffea arabica seed extract is rich in caffeine and chlorogenic acid, which can scavenge free radicals, promote microcirculation, help excrete metabolic wastes, and at the same time promote keratin renewal, assist in softening follicular horn plugs, and reduce the formation of whiteheads. Matrine regulates the TLR4 / MyD88 signaling pathway, inhibits the inflammatory response caused by Propionibacterium acnes, and balances the skin immune response, effectively reducing sensitive pore problems caused by overactive immunity. Based on the above-mentioned effects of the components, there are still unpredictable synergistic or antagonistic effects between the components. However, based on the component combination and parts by mass of the components provided by the present invention, the effects of the present invention can be achieved.

[0009] As a preferred embodiment of the pore cleansing composition of the present invention, the pore cleansing composition comprises the following components in parts by mass: 0.5-5 parts of two-way fermentation product, 0.5-3 parts of guaiac extract, 0.05-0.5 parts of asiaticoside, 0.1-1 part of Coffea arabica seed extract, and 0.05-0.5 parts of matrine.

[0010] As a preferred embodiment of the pore cleansing composition of the present invention, the pore cleansing composition comprises the following components in parts by mass: 1-2 parts of two-way fermentation product, 1-2 parts of guaiac extract, 0.1-0.2 parts of asiaticoside, 0.3-0.5 part of Coffea arabica seed extract, and 0.2-0.3 parts of matrine.

[0011] The present invention has found through research that when the mass parts of the components in the pore cleansing composition are further selected within the above ranges, the comprehensive performance of the obtained product is better.

[0012] As a preferred embodiment of the pore cleansing composition of the present invention, based on the total mass of the pore cleansing composition, the sum of the mass percentages of asiaticoside and matrine is 1-34%.

[0013] Exemplarily, based on the total mass of the pore cleansing composition, the sum of the mass percentages of asiaticoside and matrine can be any point value or any two-point range value between 1-34%, such as 1%, 3%, 5%, 8%, 10%, 12%, 15%, 18%, 20%, 22%, 25%, 28%, 30%, 34%, etc.

[0014] Preferably, based on the total mass of the pore cleansing composition, the sum of the mass percentages of asiaticoside and matrine is 7-14%. For example, it can be 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, etc.

[0015] The present invention has found through research that the sum of the mass percentages of asiaticoside and matrine in the pore cleansing composition will, to a certain extent, affect the anti-inflammatory effect of the pore cleansing composition and the effect of preventing keratin accumulation; thus, it will affect the effect of removing blackheads and whiteheads when applied to the lotion, and will also affect the pore shrinking effect; when the sum of the mass percentages of asiaticoside and matrine is further selected within the above ranges, the comprehensive effect of the obtained pore cleansing composition is better.

[0016] As a preferred embodiment of the pore cleansing composition of the present invention, based on the total mass of the pore cleansing composition, the mass percentage of the small fruit coffee seed extract ≥ 7%.

[0017] Exemplarily, based on the total mass of the pore cleansing composition, the mass percentage of the small fruit coffee seed extract can be any point value or any two-point range value ≥ 7%, such as 7-14%, 7-19%, 7-34%, 7-38%, etc.; or it can be 7%, 9%, 10%, 12%, 14%, 16%, 18%, 20%, 22%, 24%, 26%, 28%, 30%, 32%, 34%, 36%, 38%, etc.

[0018] Preferably, based on the total mass of the pore cleansing composition, the mass percentage of the small fruit coffee seed extract is 7-14%.

[0019] The present invention has found through research that when the mass percentage of the small fruit coffee seed extract is further selected within the above ranges, the comprehensive effect of the obtained plant extract is better.

[0020] As a preferred embodiment of the pore cleansing composition of the present invention, the preparation method of the bidirectional fermentation product of Phellinus officinalis-Lactobacillus plantarum comprises the following steps:

[0021] (1) Mix Phellinus officinalis and Lactobacillus plantarum bacterial liquid and perform anaerobic fermentation to obtain a fermentation product;

[0022] (2) Cool the fermentation product to 2-6 °C and then perform cyclic treatment in a microfluidic homogenizer, and finally break and centrifuge, collect the supernatant and dry it to obtain the bidirectional fermentation product of Phellinus officinalis-Lactobacillus plantarum.

[0023] The present invention has found through research that when Phellinus officinalis and Lactobacillus plantarum are subjected to bidirectional fermentation in the above manner, under the action of Lactobacillus plantarum, Phellinus officinalis not only has a higher extraction efficiency, but also the obtained bidirectional fermentation product may produce additional active ingredients that were not present before fermentation, and it can better interact with other components, thereby improving the comprehensive effect of the pore cleansing composition.

[0024] As a preferred embodiment of the pore cleansing composition of the present invention, in the step (1), the mass ratio of Phellinus officinalis to Lactobacillus plantarum bacterial liquid is 1:(4-6).

[0025] Exemplarily, the mass ratio of Phellinus officinalis to Lactobacillus plantarum bacterial liquid can be any point value or any two-point range value between 1:(4-6), such as 1:4, 1:5, 1:6, etc.

[0026] Preferably, in the step (1), the mass ratio of Phellinus officinalis to Lactobacillus plantarum bacterial liquid is 1:5.

[0027] The present invention speculates that during the preparation process of the bidirectional fermentation product, the mass ratio of Phellinus officinalis to Lactobacillus plantarum bacterial liquid will, to a certain extent, affect the types and contents of active ingredients in the fermentation product. When the mass ratio of the two is further selected within the above range, when the obtained bidirectional fermentation product is applied to the subsequent pore cleansing composition, the comprehensive effect of the obtained pore cleansing composition is better.

[0028] It should be noted that before mixing the Phellinus officinalis with the Lactobacillus plantarum fermentation broth, it also includes a pretreatment step; the pretreatment of the Phellinus officinalis includes: crushing the dried fruiting body of Phellinus officinalis to 40-60 mesh and then sterilizing.

[0029] As a preferred embodiment of the pore cleansing composition of the present invention, in the step (1), the temperature of anaerobic fermentation is 37±2 °C, and the time of anaerobic fermentation is 48-72 h.

[0030] Exemplarily, the time of the anaerobic fermentation can be any point value or any two-point range value between 48 and 72 h, such as 48 h, 60 h, 72 h, etc.

[0031] As a preferred embodiment of the pore cleansing composition of the present invention, in the step (1), based on the total mass of the medicinal Phellinus linteus and the Lactobacillus plantarum bacterial liquid, 1-3% g / mL of glucose and 0.03-0.07% g / mL of magnesium sulfate are further added for anaerobic fermentation.

[0032] As a preferred embodiment of the pore cleansing composition of the present invention, in the step (2), the pressure of the cyclic treatment is 1000-1500 bar, the temperature of the cyclic treatment is <40 °C, and the number of cyclic treatments is 3-5 times.

[0033] Preferably, in the step (2), the pressure of the cyclic treatment is 1200-1400 bar, and the temperature of the cyclic treatment is 20-30 °C.

[0034] Preferably, in the step (2), during the cyclic treatment by the microfluidic homogenizer, the stirring speed is 500-800 rpm.

[0035] The present invention has found through research that during the low-temperature microfluidic extraction process in step (2), after cooling the fermentation product and then extracting within the above parameter range, while improving the extraction efficiency, active components that can synergize with other components can be effectively extracted, thereby enhancing the comprehensive effect of the pore cleansing composition.

[0036] Preferably, the rotation speed of the crushing and centrifugation is 10000-14000 g, and the time is 10-30 min.

[0037] As a preferred embodiment of the pore cleansing composition of the present invention, the preparation method of the Lactobacillus plantarum bacterial liquid includes the following steps: inoculating Lactobacillus plantarum into MRS liquid medium and anaerobically fermenting until the OD600 value is 1.2-1.5 to obtain the Lactobacillus plantarum bacterial liquid.

[0038] Preferably, the inoculation amount of the Lactobacillus plantarum is 1-5% (V / V).

[0039] Preferably, the temperature of the anaerobic fermentation is 37±2 °C, and the time of the anaerobic fermentation is 18-24 h.

[0040] Preferably, the Lactobacillus plantarum is any one of ATCC 14917 and ATCC 8014.

[0041] In the second aspect of the present invention, the present invention provides the application of the pore cleansing composition in the preparation of skin care products.

[0042] The pore cleansing composition provided by the present invention can inhibit sebum oxidation, prevent keratin accumulation, and have anti-inflammatory and bactericidal effects, thereby being able to effectively, rapidly, gently and long-lastingly reduce blackheads and whiteheads and shrink pores; therefore, it can be widely applied to the preparation of skin care products.

[0043] As a preferred embodiment of the application described in the present invention, the skin care product includes any one of lotion, emulsion, cream, mask, essence, and spray.

[0044] In the third aspect of the present invention, the present invention provides a lotion, which comprises the following components in mass percentages: 1-10% of the pore cleansing composition described in the present invention, 0.1-1% of a thickener, 0.5-5% of a humectant, 0.01-0.3% of a pH regulator, 0.5-3% of a preservative, and the balance of deionized water.

[0045] As a preferred embodiment of the lotion described in the present invention, the lotion comprises the following components in mass percentages: 4-6% of the pore cleansing composition described in the present invention, 0.2-0.5% of a thickener, 2.5-4% of a humectant, 0.03-0.08% of a pH regulator, 0.5-1.5% of a preservative, and the balance of deionized water.

[0046] As a preferred embodiment of the lotion described in the present invention, the thickener includes at least one of polyacrylate cross-linked polymer-6, carbomer, carrageenan, gellan gum, xanthan gum, microcrystalline cellulose, cellulose gum, ethyl cellulose, Caesalpinia spinosa gum, guar gum, acryloyldimethyltaurine / VP copolymer, and acrylate copolymer.

[0047] As a preferred embodiment of the lotion described in the present invention, the humectant includes at least one of glycerin, D-panthenol, vitamin B5, 1,3-butanediol, 1,2-hexanediol, 1,3-propanediol, sodium hyaluronate, tremella polysaccharide, trehalose, betaine, allantoin, and low molecular weight sodium hyaluronate.

[0048] As a preferred embodiment of the lotion described in the present invention, the pH regulator includes at least one of arginine, disodium ethylenediaminetetraacetate, tromethamine, and EDTA disodium.

[0049] As a preferred embodiment of the lotion described in the present invention, the preservative includes at least one of hydroxyacetophenone and polyhydric alcohol.

[0050] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0051] By selecting components with appropriate parts by mass, the components interact with each other and cooperate together, and can synergistically act through multiple channels such as inhibiting sebum oxidation, preventing keratin accumulation, anti-inflammation, and sterilization, so as to achieve rapid and long-lasting reduction of blackheads and whiteheads, and the effect of shrinking pores; at the same time, the pore cleansing composition provided by the present invention is mild and non-irritating, and is particularly suitable for people with sensitive skin. BRIEF DESCRIPTION OF THE DRAWINGS

[0052] Figure 1 It is a comparison diagram of the improvement of blackheads and whiteheads in the nose area of the volunteers in the blank application example and application example 1 of the present invention before and after use. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0053] To better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0054] The reagents, methods and equipment used in the present invention are all conventional reagents, methods and equipment in the art without special instructions; and without special instructions, the raw materials used in parallel experiments are the same batch of raw materials.

[0055] Guaiac extract: Purchased from Zhenghe (Guangzhou) Biotechnology Co., Ltd.

[0056] Coffea arabica seed extract: Purchased from Xi'an Qingshuo Import and Export Trading Co., Ltd.

[0057] Madecassoside: Purchased from Shaanxi Wanhe Pharmaceutical Co., Ltd., with a purity of 98%.

[0058] Matrine: Purchased from Shanxi Xinyu Biotechnology Co., Ltd., with a purity of 98%.

[0059] Two-way fermentation product 1: Self-made, and the preparation method includes the following steps:

[0060] (1) Inoculate Lactobacillus plantarum (ATCC 8014) into MRS liquid medium (purchased from Thermo Fisher) at an inoculation amount of 3% (V / V), and anaerobically ferment at 37°C for 20 h until the OD600 value is 1.4 to obtain Lactobacillus plantarum bacterial liquid;

[0061] (2) Crush the dried fruiting bodies of Phellinus linteus (purchased from Anhui Boman Pharmaceutical Co., Ltd.) to 40 mesh and sterilize at 121°C for 15 min to obtain Phellinus linteus powder;

[0062] (3) Mix the Phellinus linteus powder and the Lactobacillus plantarum bacterial liquid at a mass ratio of 1:5, and add 2% g / mL mass-volume concentration of glucose and 0.05% g / mL mass-volume concentration of magnesium sulfate based on the total volume of the Phellinus linteus powder and the Lactobacillus plantarum bacterial liquid, and then anaerobically ferment at 37°C for 60 h to obtain a fermentation product;

[0063] (4) Pre-cool the fermentation product to 4°C, then use a microfluidic homogenizer to perform cyclic treatment 4 times at a pressure of 1200 bar, a stirring speed of 600 rpm, and a temperature of 25°C. After the cyclic treatment is completed, break it, then centrifuge at a speed of 12000 g for 20 min, collect the supernatant and vacuum dry it at 25°C to obtain the two-way fermentation product 1.

[0064] Two-way fermentation product 2: Self-made, and the preparation method includes the following steps:

[0065] (1) Inoculate Lactobacillus plantarum (ATCC 14917) into MRS liquid medium at an inoculation amount of 3% (V / V), and anaerobically ferment at 37°C for 18 h until the OD600 value reaches 1.2 to obtain Lactobacillus plantarum bacterial liquid;

[0066] (2) Crush the dried fruiting bodies of Phellinus linteus (purchased from Anhui Boman Pharmaceutical Co., Ltd.) to 40 meshes, then sterilize at 121°C for 15 min to obtain Phellinus linteus powder;

[0067] (3) Mix the Phellinus linteus powder and Lactobacillus plantarum bacterial liquid in a mass ratio of 1:6, and add glucose with a mass-volume concentration of 2% g / mL and magnesium sulfate with a mass-volume concentration of 0.05% g / mL based on the total volume of the Phellinus linteus powder and Lactobacillus plantarum bacterial liquid. Then anaerobically ferment at 37°C for 72 h to obtain the fermentation product;

[0068] (4) Pre-cool the fermentation product to 4°C, then use a microfluidic homogenizer to perform cyclic treatment 5 times at a pressure of 1400 bar, a stirring speed of 600 rpm, and a temperature of 20°C. After the cyclic treatment is completed, break it, then centrifuge at a speed of 12000 g for 20 min, collect the supernatant and vacuum dry it at 25°C to obtain the two-way fermentation product 2.

[0069] Two-way fermentation product 3: Self-made, and the difference in the preparation method from the two-way fermentation product 1 is that the mass ratio of Phellinus linteus powder to Lactobacillus plantarum bacterial liquid is 1:3.

[0070] Two-way fermentation product 4: Self-made, and the difference in the preparation method from the two-way fermentation product 1 is that the mass ratio of Phellinus linteus powder to Lactobacillus plantarum bacterial liquid is 1:8.

[0071] Two-way fermentation product 5: Self-made, and the difference in the preparation method from the two-way fermentation product 1 is that it is cyclically treated 4 times at a pressure of 1200 bar and a temperature of 45°C using a microfluidic homogenizer.

[0072] Two-way fermentation product 6: Self-made, and the preparation method includes the following steps:

[0073] (1) Inoculate Lactobacillus plantarum (ATCC 8014) into MRS liquid medium at an inoculum size of 3% (V / V), and anaerobically ferment at 37°C for 20 h until the OD600 value reaches 1.4 to obtain a Lactobacillus plantarum bacterial solution;

[0074] (2) Crush the dried fruiting bodies of Phellinus linteus (purchased from Anhui Boman Pharmaceutical Co., Ltd.) to 40 mesh, and sterilize at 121°C for 15 min to obtain Phellinus linteus powder;

[0075] (3) Mix the Phellinus linteus powder and the Lactobacillus plantarum bacterial solution at a mass ratio of 1:5, and add glucose with a mass - volume concentration of 2% g / mL and magnesium sulfate with a mass - volume concentration of 0.05% g / mL based on the total volume of the Phellinus linteus powder and the Lactobacillus plantarum bacterial solution. Then, anaerobically ferment at 37°C for 60 h to obtain a fermentation product;

[0076] (4) Centrifuge the fermentation product at a speed of 12000 g for 20 min, collect the supernatant and vacuum - dry it at 25°C to obtain the double - fermentation product 6.

[0077] Double - fermentation product 7: Self - made. The preparation method is different from that of double - fermentation product 1 in that yeast (ATCC204508) is used to replace Lactobacillus plantarum.

[0078] Double - fermentation product 8: Self - made. The preparation method is different from that of double - fermentation product 1 in that Phellinus linteus (purchased from Shaanxi Haosen Biotechnology Co., Ltd.) is used to replace Phellinus linteus.

[0079] Examples 1 - 8 and Comparative Examples 1 - 8

[0080] The examples and comparative examples of the present invention provide a pore - cleansing composition. The components (parts by mass) of the pore - cleansing composition are shown in Table 1; wherein W1 represents the sum of the mass percentages of asiaticoside and matrine based on the total mass parts of the pore - cleansing composition, and W2 represents the mass percentage of small fruit coffee seed extract based on the total mass parts of the pore - cleansing composition.

[0081] Table 1

[0082]

[0083]

[0084] The preparation method of the pore - cleansing composition provided in Example 1 is: Mix the components to obtain the pore - cleansing composition.

[0085] The preparation methods of the pore - cleansing compositions provided in Examples 2 - 8 and Comparative Examples 1 - 8 are the same as that of Example 1; if there are no relevant components, do not add them.

[0086] Example 9

[0087] An embodiment of the present invention provides a pore cleansing composition. The only difference between this pore cleansing composition and that of Example 1 is that the two-way fermentation product 2 is used to replace the two-way fermentation product 1.

[0088] Example 10

[0089] An embodiment of the present invention provides a pore cleansing composition. The only difference between this pore cleansing composition and that of Example 1 is that the two-way fermentation product 3 is used to replace the two-way fermentation product 1.

[0090] Example 11

[0091] An embodiment of the present invention provides a pore cleansing composition. The only difference between this pore cleansing composition and that of Example 1 is that the two-way fermentation product 4 is used to replace the two-way fermentation product 1.

[0092] Example 12

[0093] An embodiment of the present invention provides a pore cleansing composition. The only difference between this pore cleansing composition and that of Example 1 is that the two-way fermentation product 5 is used to replace the two-way fermentation product 1.

[0094] Example 13

[0095] An embodiment of the present invention provides a pore cleansing composition. The only difference between this pore cleansing composition and that of Example 1 is that the two-way fermentation product 6 is used to replace the two-way fermentation product 1.

[0096] Comparative Example 9

[0097] A comparative example of the present invention provides a pore cleansing composition. The only difference between this pore cleansing composition and that of Example 1 is that the two-way fermentation product 7 is used to replace the two-way fermentation product 1.

[0098] Comparative Example 10

[0099] A comparative example of the present invention provides a pore cleansing composition. The only difference between this pore cleansing composition and that of Example 1 is that the two-way fermentation product 8 is used to replace the two-way fermentation product 1.

[0100] Comparative Example 11

[0101] A comparative example of the present invention provides a pore cleansing composition. The only difference between this pore cleansing composition and that of Example 1 is that the Brazilian pau d'arco extract (purchased from Shaanxi Lvshengyuan Biological Products Manufacturing Co., Ltd.) is used to replace the guaiac wood extract.

[0102] Comparative Example 12

[0103] The comparative example of the present invention provides a pore cleansing composition. The only difference between the pore cleansing composition and Example 1 is that asiatic acid (purchased from Shaanxi Wanhe Pharmaceutical Co., Ltd.) is used to replace madecassoside.

[0104] Comparative Example 13

[0105] The comparative example of the present invention provides a pore cleansing composition. The only difference between the pore cleansing composition and Example 1 is that cocoa seed extract (purchased from Xi'an Shouhe Biotechnology Co., Ltd.) is used to replace coffea arabica seed extract.

[0106] Comparative Example 14

[0107] The comparative example of the present invention provides a pore cleansing composition. The only difference between the pore cleansing composition and Example 1 is that betaine (purchased from Shandong Jinyou Biotechnology Co., Ltd.) is used to replace matrine. Application Examples 1-15, Comparative Application Examples 1-14 and Blank Application Example

[0108] The application examples, comparative application examples and blank application example of the present invention provide a lotion. The components (mass percentages) of the lotion are shown in Table 2; among them, the pore cleansing compositions used in Application Examples 1-13 are the pore cleansing compositions prepared in Examples 1-13 respectively. For example, the pore cleansing composition used in Application Example 1 is the pore cleansing composition in Example 1, the pore cleansing composition used in Application Example 2 is the pore cleansing composition in Example 2, and so on; the pore cleansing compositions used in Comparative Application Examples 1-14 are the pore cleansing compositions prepared in Comparative Examples 1-14 respectively; the pore cleansing compositions used in Application Examples 14-15 are the pore cleansing compositions prepared in Example 1.

[0109] Table 2

[0110]

[0111]

[0112] The preparation method of the lotion provided by Application Example 1 includes the following steps:

[0113] (1) Mix the humectant and thickener with water and stir. After heating to 85 °C, homogenize at a speed of 1300 rpm for 4 min to obtain prefabricated component A;

[0114] (2) Mix the preservatives and heat to 60 °C to melt to obtain prefabricated component B;

[0115] (3) After cooling the prefabricated Component A to 60°C, add the prefabricated Component B and stir to mix at a rotation speed of 300 rpm. Then cool it to below 45°C, add the pore cleansing composition and continue stirring for 8 minutes. Finally, add a pH regulator to adjust the pH to 5.5 - 6.0, then stop stirring, discharge the material, and obtain the lotion.

[0116] The preparation methods of the emulsions provided in Application Examples 2 - 15, Comparative Application Examples 1 - 14, and Blank Application Example are the same as those in Application Example 1. Just do not add the relevant components if there are no such components.

[0117] Effect Example 1

[0118] The effect examples of the present invention explore the effects of the emotional beauty compositions prepared in Examples 1 - 13 and Comparative Examples 1 - 14, including the following aspects:

[0119] 1. Ability to inhibit sebum oxidation in vitro

[0120] This test uses the thiobarbituric acid reactive substances (TBARs) method to evaluate the ability of the sample to inhibit sebum oxidation and the ability to remove blackheads; the end product of sebum oxidation is mainly malondialdehyde, and these peroxidation products react with thiobarbituric acid to form a colored compound, which has an absorption peak at 530 nm. Therefore, the sebum oxidation level can be evaluated by detecting the absorbance at 530 nm.

[0121] The test method is as follows:

[0122] (1) Prepare artificial sebum: Mix squalene, triglyceride, and wax ester evenly at a ratio of 4:3:3 to obtain artificial sebum;

[0123] (2) Prepare a thiobarbituric acid solution: Mix a 0.1 mol / L hydrochloric acid aqueous solution and a 1% thiobarbituric acid aqueous solution at a volume ratio of 2:1, heat and boil until the thiobarbituric acid is completely dissolved, keep warm for standby, and obtain the thiobarbituric acid solution;

[0124] (3) Add the pore cleansing compositions prepared in the examples and comparative examples (the blank control group does not add the composition) to the artificial sebum (calculated based on the mass of the artificial sebum, the mass percentage of the pore cleansing composition is 1%), mix evenly, and incubate at 75°C, 60% RH, and in a light environment for 24 hours;

[0125] (4) Mix the incubated reaction solution and the thiobarbituric acid solution evenly at a volume ratio of 7:3, water bath at 65°C for 1 hour, centrifuge, take the supernatant, and measure the absorbance OD value at 530 nm.

[0126] Sebum is prone to oxidation and blackening when exposed to air, eventually forming blackheads. Therefore, the lower the oxidation level of sebum, the stronger the ability of the sample to inhibit sebum oxidation and the stronger the ability to remove blackheads. The ability of the composition to remove blackheads is indicated by the reduction in the sebum oxidation level, and the calculation formula is as follows: Reduction rate of sebum oxidation level (%) = (1 - OD value of the sample group / OD value of the blank control group) * 100%;

[0127] The results obtained are shown in Table 3.

[0128] 2. Ability to prevent keratinocyte accumulation in vitro

[0129] Experimental materials: The cell line used was human keratinocyte HaCaT (Guangzhou Geneo Biotechnology Co., Ltd.). The test conditions were: incubator temperature 37 ± 1 °C, humidity 90 ± 5%, carbon dioxide 5 ± 1%; Cell culture and treatment were carried out according to the grouping, and then the test was carried out. The specific test was the detection of keratin K10 expression.

[0130] The specific test method is as follows:

[0131] (1) Inoculate the cell suspension into a 96-well cell culture plate at a density of 2000 cells / well, add 100 μL of culture medium to each well, and culture for 24 h;

[0132] (2) Discard the supernatant. The blank control group was added with 100 μL of DMEM medium (2105341, Gibco), and the sample groups were respectively added with 100 μL of medium containing 1% g / mL of the corresponding sample (the pore-cleansing composition prepared in the examples and comparative examples), and the cells were collected after continuing to culture for 48 h;

[0133] (3) Detection of keratin K10 content: The cells were lysed with RIPA cell lysate containing protease inhibitor. The lysed sample was centrifuged at 12000 g for 5 minutes, and the supernatant was taken. The keratin K10 content in the cell supernatant of each group was detected using a human keratin 10 (KRT10) ELISA detection kit (product number JL32475-96T, Jianglai Biotech).

[0134] Excessive accumulation of keratinocytes can lead to the formation of plugs, clog pores, and cause sebum to accumulate inside the pores, forming whiteheads. Keratin K10 is mainly expressed during the differentiation stage of keratinocytes, participates in the formation of a stable intermediate filament network, provides mechanical strength to the stratum corneum, and ensures the orderly shedding of keratinocytes. Therefore, the higher the expression of keratin K10, the more successful the differentiation and shedding of keratinocytes, and the stronger the ability of the sample to remove whiteheads; while the lower the expression of keratin K10, the more blocked the differentiation and the more keratinocytes accumulate, and the weaker the ability of the sample to remove whiteheads. The ability of the composition to remove whiteheads is represented by the increase in keratin K10, and the calculation formula is as follows: Keratin K10 increase rate = (sample group - blank control group) / blank control group × 100%;

[0135] The results obtained are shown in Table 3 below.

[0136] Table 3

[0137]

[0138] As can be seen from Table 3, when the technical solution provided by the present invention is adopted, the obtained pore cleansing composition has good ability to reduce sebum oxidation and prevent keratinocyte accumulation; specifically, the obtained product has a reduction rate of sebum oxidation level of more than 39.5%, and an increase rate of keratin K10 of more than 19.8%;

[0139] As can be seen from Examples 1-8, the mass parts of the components, the mass ratio between the components, or the mass percentage of one or two components in the composition will also affect the performance of the product. When the mass parts of the components are further selected within the preferred range given by the present invention, the comprehensive performance of the obtained product is better; specifically, the obtained product has a reduction rate of sebum oxidation level of more than 77.9%, and an increase rate of keratin K10 of more than 40.9%; As can be seen from Examples 1 and Examples 9-13, the preparation method of the two-way fermentation product will also affect the comprehensive performance of the product to a certain extent;

[0140] As can be seen from Example 1 and Comparative Examples 1-8, when one or two components are not added, or when other component parts are supplemented while not adding, the effects of the present invention cannot be achieved; as can be seen from Example 1 and Comparative Examples 9-10, when the raw materials of the two-way fermentation product are not the substances provided by the present invention, the effects of the present invention cannot be achieved; as can be seen from Example 1 and Comparative Examples 11-14, when other similar components are used for substitution, the corresponding effects of the present invention cannot be obtained either.

[0141] Effect Example 2

[0142] This invention's efficacy example explores the safety of the lotion prepared from Application Examples 1 - 15, Comparative Application Examples 1 - 14, and Blank Application Example. Specifically, it is tested by the human skin patch test:

[0143] Recruit 30 volunteers, 15 males and 15 females, aged 20 - 50 years old. Using the closed patch test method, place an equal amount (0.020 mL - 0.025 mL) of the test sample (the lotion prepared from the application example) in a specific patch tester, apply it to the volunteers' arms with a low - sensitization tape, gently press to make it evenly adhere to the skin, and keep it for 24 h; the blank control group is distilled water. After 24 h, remove the patch tester, and observe the skin reaction at 0.5 h, 24 h, and 48 h, and record the results. The adverse reaction grades of the skin are shown in Table 4 below;

[0144] Table 4

[0145]

[0146] The results show that after the human patch test, the lotions prepared from Application Examples 1 - 15, Comparative Application Examples 1 - 14, and Blank Application Example are all negative reactions, and they are not irritating to the human skin and are safe.

[0147] Efficacy Example 3

[0148] This invention's efficacy example explores the application effects of the lotions prepared from Application Examples 1 - 15, Comparative Application Examples 1 - 14, and Blank Application Example on humans, including the following steps:

[0149] Select 180 Asian adult trial participants aged 18 - 60 years old with obvious blackheads, whiteheads, and accompanied by enlarged pores and sensitive skin according to the "Technical Specifications for Cosmetics Safety" (2015). Randomly divide them into 30 groups, with 6 people in each group. The volunteers use the samples (the samples are the lotions prepared from the application examples, comparative application examples, and blank application example) on the whole face twice a day (morning and evening) after regular facial cleansing. Data is collected on the 0th day, 7th day, 28th day, and 5 days after discontinuation (5 days after discontinuation means not using the corresponding lotion after regular facial cleansing). After the volunteers arrive, wash their faces with facial cleanser and sit quietly in an air - conditioned room at a temperature of 21 ± 1 °C and a humidity of 50 ± 10% for 30 minutes. The researcher uses VISIA - CR to take pictures and analyze the volunteers' faces, and calculates the number of blackheads and whiteheads according to the images; uses the skin image analyzer digimiC800 to measure the skin pore diameter on the outside of the nasolabial groove.

[0150] The blackhead - removing ability of the composition is represented by the reduction of the number of blackheads, the whitehead - removing ability is represented by the reduction of the number of whiteheads, and the pore - shrinking ability is represented by the reduction of the pore diameter. The formula is as follows:

[0151] Improvement rate=(T0 样品组-Tx 样品组 ) / T0 样品组 *100%]-[(T0 空白应用例 -Tx 空白应用例 ) / T0 空白应用例 *100%;

[0152] The results obtained are shown in Table 5;

[0153] Table 5

[0154]

[0155]

[0156] It can be seen from Table 5 that when the technical solution provided by the present invention is adopted, the obtained lotion has a good effect of reducing blackheads and whiteheads and shrinking pores, and the obtained product has a rapid and lasting effect on reducing blackheads and whiteheads and shrinking pores; specifically, the improvement rates of the obtained lotion on blackheads and whiteheads at T7 are above 12.9% and 14.9% respectively, and the improvement rate on pores is above 11.5%; at T28, the improvement rates on blackheads and whiteheads are above 29.7% and 34.9% respectively, and the improvement rate on pores is above 20.6%; when stopped using at T5, the improvement rates on blackheads and whiteheads are above 11.4% and 14.1% respectively, and the improvement rate on pores is above 11.1%;

[0157] It can be seen from Application Examples 1-8 that the mass parts of the components in the pore-cleansing composition added to the lotion, the mass ratio between the components, or the mass percentage of one or two components in the composition will also affect the performance of the lotion. When the mass parts of the components in the pore-cleansing composition are further selected within the preferred range given in the present invention, the comprehensive performance of the obtained lotion is better; specifically, the improvement rates of the obtained lotion on blackheads and whiteheads at T7 are above 53.9% and 60.6% respectively, and the improvement rate on pores is above 32.3%; at T28, the improvement rates on blackheads and whiteheads are above 60.6% and 69.2% respectively, and the improvement rate on pores is above 34.4%; when stopped using at T5, the improvement rates on blackheads and whiteheads are above 49.9% and 56.1% respectively, and the improvement rate on pores is above 31.1%;

[0158] It can be seen from Application Example 1 and Application Examples 9-13 that the preparation method of the two-way fermentation product will also affect the comprehensive performance of the lotion to a certain extent;

[0159] As can be seen from Application Example 1 and Comparative Application Examples 1-8, when one or two of the components are not added to the pore cleansing composition, or when the other components are used to make up for the non-addition, the effects of the present invention cannot be achieved; as can be seen from Application Example 1 and Comparative Application Examples 9-14, when other similar components are used to replace the raw materials in the two-way fermentation product or other similar components are used to replace other components, the corresponding effects of the present invention cannot be obtained either.

[0160] Meanwhile, a volunteer was randomly selected from Application Example 1 and the blank application example to give test pictures before and after use, as Figure 1 shown, where the black arrow indicates the position of the blackhead and the red arrow indicates the position of the whitehead; it can also be clearly seen from Figure 1 that when the lotion prepared by the present invention is used for 28 days, it can effectively reduce blackheads and whiteheads.

[0161] Finally, it should be noted that the above embodiments are used to illustrate the technical solutions of the present invention rather than to limit the protection scope of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A pore cleansing composition containing Phellinus linteus, characterized in that, The pore-cleansing composition comprises the following components in parts by mass: Two-way fermentation product 0.1 - 10 parts, guaiac extract 0.1 - 5 parts, asiaticoside 0.01 - 1 part, coffee arabica seed extract 0.01 - 3 parts, matrine 0.01 - 2 parts; The two-way fermentation product is a medicinal phellinus linteus - lactobacillus plantarum two-way fermentation product.

2. The pore cleansing composition according to claim 1, wherein The pore-cleansing composition comprises the following components in parts by mass: Two-way fermentation product 0.5 - 5 parts, guaiac extract 0.5 - 3 parts, asiaticoside 0.05 - 0.5 part, coffee arabica seed extract 0.1 - 1 part, matrine 0.05 - 0.5 part.

3. The pore cleansing composition according to claim 1, characterized in that, The pore-cleansing composition comprises the following components in parts by mass: Two-way fermentation product 1 - 2 parts, guaiac extract 1 - 2 parts, asiaticoside 0.1 - 0.2 part, coffee arabica seed extract 0.3 - 0.5 part, matrine 0.2 - 0.3 part.

4. The pore cleansing composition according to claim 1, characterized in that, Based on the total mass of the pore-cleansing composition, the sum of the mass percentages of asiaticoside and matrine is 1 - 34%; And / or, based on the total mass of the pore-cleansing composition, the mass percentage of coffee arabica seed extract ≥ 7%.

5. The pore cleansing composition according to claim 1, characterized in that, The preparation method of the medicinal phellinus linteus - lactobacillus plantarum two-way fermentation product comprises the following steps: (1) Mix the medicinal phellinus linteus and the lactobacillus plantarum bacterial liquid and perform anaerobic fermentation to obtain a fermentation product; (2) Cool the fermentation product to 2 - 6 °C and then perform cyclic treatment in a microfluidic homogenizer, finally crush and centrifuge, collect the supernatant and dry it to obtain the medicinal phellinus linteus - lactobacillus plantarum two-way fermentation product.

6. The pore cleansing composition according to claim 5, characterized in that, In the step (1), the mass ratio of the medicinal phellinus linteus to the lactobacillus plantarum bacterial liquid is 1:(4 - 6); And / or, in the step (1), the temperature of the anaerobic fermentation is 37 ± 2 °C, and the time of the anaerobic fermentation is 48 - 72 h; And / or, in the step (1), based on the total volume of the medicinal phellinus linteus and the lactobacillus plantarum bacterial liquid, 1 - 3% g / mL of glucose with a mass-volume concentration and 0.03 - 0.07% g / mL of magnesium sulfate with a mass-volume concentration are further added for anaerobic fermentation.

7. The pore cleansing composition according to claim 5, wherein In the step (2), the pressure of the cyclic treatment is 1000 - 1500 bar, the temperature of the cyclic treatment is < 40 °C, and the number of times of the cyclic treatment is 3 - 5 times.

8. The pore cleansing composition according to claim 5, wherein The preparation method of the lactobacillus plantarum bacterial liquid comprises the following steps: Inoculate lactobacillus plantarum into an MRS liquid medium and perform anaerobic fermentation until the OD600 value is 1.2 - 1.5 to obtain the lactobacillus plantarum bacterial liquid.

9. Use of the pore-cleansing composition according to any one of claims 1 - 8 in the preparation of skin care products.

10. A lotion, characterized in that, The lotion comprises the following components in mass percentages: 1 - 10% of the pore-cleansing composition according to any one of claims 1 - 8, 0.1 - 1% of a thickening agent, 0.5 - 5% of a moisturizing agent, 0.01 - 0.3% of a pH regulator, 0.5 - 3% of a preservative, and the balance deionized water.

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