Pressure-relieving compound essential oil as well as preparation method and application thereof

Abstract

The invention discloses pressure-relieving compound essential oil as well as a preparation method and application thereof. The pressure-relieving compound essential oil is prepared from the following components in parts by volume: 60 to 100 parts of lavender essential oil, 0.01 to 20 parts of Roman chamomile essential oil, 0.01 to 10 parts of peppermint essential oil, 0.01 to 10 parts of balm essential oil and 0.01 to 15 parts of verbenone rosemary essential oil. The pressure-relieving compound essential oil disclosed by the invention is scientific and rigorous in proportion, not only can achieve a good pressure-relieving effect, but also is fresh and comfortable in fragrance and good in user experience; through combination of sensory evaluation and network pharmacological research, the compound essential oil is scientific and rigorous in proportion and fresh and comfortable in fragrance, and through brain wave and saliva cortisol detection, the prepared compound essential oil has the effects of relieving pressure and relaxing nervous mood and is good in application prospect.

Description

Technical Field

[0001] The present invention relates to the technical field of fragrance and essence, and relates to a stress-relieving compound essential oil and its preparation method and application. Background Art

[0002] The dual pressures from life and work have put modern people in a long-term stress state. If not relieved and adjusted in a timely and effective manner, it is likely to trigger a series of physical and mental health problems, such as insomnia, anxiety, mania, depression, etc., which have an adverse impact on work and life. Western medicine intervention is the most commonly used treatment method for depression in clinical practice. Targeted drugs can quickly play a certain regulatory role in neurological diseases. However, it cannot be ignored that they will also have negative effects such as drug resistance and an increase in the dosage required by patients in the later stage, and the long-term treatment is difficult. Aromatherapy is to inhale essential oils, so that the aroma molecules pass through the olfactory system and the blood-brain barrier, affecting the central nervous system, and playing roles such as relieving stress, reducing pain, and improving sleep. In recent years, it has been gradually popularized and used in clinical practice as an adjuvant treatment method. It has high safety, low side effects, and has many advantages such as simple operation and low cost. However, some of the products on the market lack scientific research on efficacy. Currently, natural essential oils are generally selected as fragrances. Natural essential oils are extracted from aromatic plants, with a strong aroma intensity, and consumers have limited acceptance of some unpleasant odors, which results in the current aromatherapy products being unable to meet the market demand.

[0003] Therefore, it is of great practical significance to develop an essential oil with good user experience and good stress-relieving effect. Summary of the Invention

[0004] Due to the above-mentioned defects in the prior art, the present invention provides an essential oil with good user experience and good stress-relieving effect, specifically a stress-relieving compound essential oil and its preparation method and application, which has good safety and helps to relieve stress.

[0005] In order to achieve the above object, the present invention provides the following technical solutions:

[0006] A stress-relieving compound essential oil, comprising the following components: lavender essential oil, Roman chamomile essential oil, peppermint essential oil, lemon balm essential oil, and verbenone rosemary essential oil.

[0007] The stress-relieving compound essential oil of the present invention is prepared by using lavender essential oil, Roman chamomile essential oil, peppermint essential oil, lemon balm essential oil, and verbenone rosemary essential oil as formulation materials. The ratio is scientific and rigorous. It can not only achieve a good stress-relieving effect, but also has a fresh and comfortable aroma, good user experience, and good application prospects.

[0008] As a preferred technical solution:

[0009] A stress-relieving compound essential oil as described above, wherein the volume ratio of lavender essential oil, Roman chamomile essential oil, peppermint essential oil, lemon balm essential oil and verbenone rosemary essential oil is 60-100:0.01-20:0.01-10:0.01-10:0.01-15.

[0010] A stress-relieving compound essential oil as described above, wherein the volume ratio of lavender essential oil, Roman chamomile essential oil, peppermint essential oil, lemon balm essential oil and verbenone rosemary essential oil is 60:15:10:10:5.

[0011] A stress-relieving compound essential oil as described above, wherein the lavender essential oil is true lavender essential oil of space blue.

[0012] A stress-relieving compound essential oil as described above, wherein the lavender essential oil, Roman chamomile essential oil, peppermint essential oil, lemon balm essential oil and verbenone rosemary essential oil are all obtained by steam distillation.

[0013] The present invention also provides a method for preparing a stress-relieving compound essential oil as described above. The raw materials are mixed evenly according to the ratio, and then sealed, refrigerated and aged to obtain the stress-relieving compound essential oil.

[0014] In addition, the present invention also provides the application of a stress-relieving compound essential oil as described above in stress-relieving products and antidepressant products.

[0015] The present invention also analyzes the components, targets and action pathways of the compound essential oil provided by the present invention to exert antidepressant effects based on network pharmacology.

[0016] The above technical solutions are only a feasible technical solution of the present invention, and the protection scope of the present invention is not limited thereto. Those skilled in the art can reasonably adjust the specific design according to actual needs.

[0017] The above invention has the following advantages or beneficial effects:

[0018] (1) The stress-relieving compound essential oil of the present invention is prepared by using lavender essential oil, Roman chamomile essential oil, peppermint essential oil, lemon balm essential oil and verbenone rosemary essential oil as formula materials. The ratio is scientific and rigorous. It can not only achieve good stress-relieving effects, but also has a fresh and comfortable aroma, and good user experience;

[0019] (2) The present invention uses electroencephalogram experiments to test the stress-relieving effect of the compound essential oil on people with depressive tendencies, and collects saliva samples at different experimental stages to detect the change level of salivary cortisol;

[0020] (3) The stress-relieving compound essential oil of the present invention has been shown by human electroencephalogram experiments to be able to reduce the β / α value of human brain waves, achieving the effect of relaxing the mood and relieving stress;

[0021] (4)The stress-relieving compound essential oil of the present invention, as shown by the detection results of human saliva cortisol, can significantly reduce the concentration of human saliva cortisol, has the effects of relieving stress and boosting mood, and has good application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] By reading the following detailed description of the non-limiting embodiments with reference to the accompanying drawings, the present invention and its features, shapes and advantages will become more obvious. The same reference numerals indicate the same parts in all the drawings. The drawings are not drawn to scale, and the emphasis is on showing the gist of the present invention.

[0023] Figure 1 For Experiment 1, the scores and popularity statistics of each dimension of the sensory evaluation experiment on Examples 1-11 and the comparative example are shown (in the figure, 1-11 correspond to Examples 1-11 respectively, and 12 corresponds to the comparative example);

[0024] Figure 2 For Experiment 2, it is a Venn diagram of the intersection of the target points of each active ingredient and the disease target points;

[0025] Figure 3 For Experiment 2, it is a KEGG enrichment analysis diagram of the intersection target points;

[0026] Figure 4 For Experiment 2, it is a GO enrichment analysis diagram of the intersection target points;

[0027] Figure 5 For Experiment 3, it is a topographic map of the brain waves of the participants during the smelling process;

[0028] Figure 6 For Experiment 3, it is a detection data diagram of salivary amylase in the saliva collected from the participants with electroencephalogram. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0029] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments, but it is not limited to the present invention.

[0030] Example 1

[0031] A preparation method of a stress-relieving compound essential oil is as follows: take 60 parts by volume of lavender essential oil, 20 parts by volume of Roman chamomile essential oil, 5 parts by volume of peppermint essential oil, 5 parts by volume of lemon balm essential oil, and 10 parts by volume of verbenone rosemary essential oil. After mixing the above essential oils evenly and standing for aging in a sealed state at 20 °C, the formula one compound essential oil is obtained.

[0032] Example 2

[0033] A preparation method of a pressure-relieving compound essential oil, specifically: take 60 parts by volume of lavender essential oil, 20 parts by volume of Roman chamomile essential oil, 5 parts by volume of peppermint essential oil, 10 parts by volume of lemon balm essential oil, and 5 parts by volume of verbenone rosemary essential oil. After mixing the above essential oils evenly and sealing and standing for aging at 20°C, the formula two compound essential oil is obtained.

[0034] Example 3

[0035] A preparation method of a pressure-relieving compound essential oil, specifically: take 60 parts by volume of lavender essential oil, 15 parts by volume of Roman chamomile essential oil, 5 parts by volume of peppermint essential oil, 10 parts by volume of lemon balm essential oil, and 10 parts by volume of verbenone rosemary essential oil. After mixing the above essential oils evenly and sealing and standing for aging at 20°C, the formula three compound essential oil is obtained.

[0036] Example 4

[0037] A preparation method of a pressure-relieving compound essential oil, specifically: take 60 parts by volume of lavender essential oil, 15 parts by volume of Roman chamomile essential oil, 10 parts by volume of peppermint essential oil, 10 parts by volume of lemon balm essential oil, and 5 parts by volume of verbenone rosemary essential oil. After mixing the above essential oils evenly and sealing and standing for aging at 20°C, the formula four compound essential oil is obtained.

[0038] Example 5

[0039] A preparation method of a pressure-relieving compound essential oil, specifically: take 60 parts by volume of lavender essential oil, 10 parts by volume of Roman chamomile essential oil, 10 parts by volume of peppermint essential oil, 5 parts by volume of lemon balm essential oil, and 10 parts by volume of verbenone rosemary essential oil. After mixing the above essential oils evenly and sealing and standing for aging at 20°C, the formula five compound essential oil is obtained.

[0040] Example 6

[0041] A preparation method of a pressure-relieving compound essential oil, specifically: take 60 parts by volume of lavender essential oil, 10 parts by volume of Roman chamomile essential oil, 5 parts by volume of peppermint essential oil, 10 parts by volume of lemon balm essential oil, and 15 parts by volume of verbenone rosemary essential oil. After mixing the above essential oils evenly and sealing and standing for aging at 20°C, the formula six compound essential oil is obtained.

[0042] Example 7

[0043] A preparation method of a pressure-relieving compound essential oil, specifically: take 60 parts by volume of lavender essential oil, 10 parts by volume of Roman chamomile essential oil, 10 parts by volume of peppermint essential oil, 10 parts by volume of lemon balm essential oil, and 10 parts by volume of verbenone rosemary essential oil. After mixing the above essential oils evenly and sealing and standing for aging at 20°C, the formula seven compound essential oil is obtained.

[0044] Example 8

[0045] A preparation method of a pressure-relieving compound essential oil, specifically: take 70 parts by volume of lavender essential oil, 10 parts by volume of Roman chamomile essential oil, 5 parts by volume of peppermint essential oil, 5 parts by volume of lemon balm essential oil, and 10 parts by volume of verbenone rosemary essential oil. After mixing the above essential oils evenly and sealing and standing for aging at 20°C, the formula eight compound essential oil is obtained.

[0046] Example 9

[0047] A preparation method of a pressure-relieving compound essential oil, specifically: take 70 parts by volume of lavender essential oil, 10 parts by volume of Roman chamomile essential oil, 5 parts by volume of peppermint essential oil, 10 parts by volume of lemon balm essential oil, and 5 parts by volume of verbenone rosemary essential oil. After mixing the above essential oils evenly and sealing and standing for aging at 20°C, the formula nine compound essential oil is obtained.

[0048] Example 10

[0049] A preparation method of a pressure-relieving compound essential oil, specifically: take 70 parts by volume of lavender essential oil, 10 parts by volume of Roman chamomile essential oil, 10 parts by volume of peppermint essential oil, 5 parts by volume of lemon balm essential oil, and 5 parts by volume of verbenone rosemary essential oil. After mixing the above essential oils evenly and sealing and standing for aging at 20°C, the formula ten compound essential oil is obtained.

[0050] Example 11

[0051] A preparation method of a pressure-relieving compound essential oil, specifically: take 80 parts by volume of lavender essential oil, 5 parts by volume of Roman chamomile essential oil, 5 parts by volume of peppermint essential oil, 5 parts by volume of lemon balm essential oil, and 5 parts by volume of verbenone rosemary essential oil. After mixing the above essential oils evenly and sealing and standing for aging at 20°C, the formula eleven compound essential oil is obtained.

[0052] Control group

[0053] A preparation method of a single essential oil, specifically: take 100 parts by volume of lavender essential oil. After mixing the above essential oil evenly and sealing and standing for aging at 20°C, the single essential oil is obtained.

[0054] To explore the comparison situation of each example and the control group, the following experiments were carried out.

[0055] Experiment 1. Aroma sensory evaluation experiment of compound essential oil

[0056] Sensory tests were conducted on the compound essential oils of the above Examples 1-11 and the single essential oil of the comparative example. Two rounds of olfactory sensory evaluations were carried out by 115 sensory evaluators without professional training from Shanghai Institute of Technology. The examples were numbered using random three-digit codes. 200 μL of the essential oil sample was injected into a 10 mL glass bottle, and scores (1-10) were given from three dimensions: aroma harmony, likability, and cool feeling. Votes were also cast for the favorite essential oils (up to three options could be selected). The sensory evaluation data were analyzed, and the average value of each score was taken, and the number of votes for the favorite essential oils was counted.

[0057] The experimental results are as Figure 1 shown. According to the scores of each formulated essential oil in the two rounds of sensory evaluations, it was calculated that Example 4, that is, the formulated essential oil of Formula 4, was the overall optimal example. Specifically, its average score for harmony was 7.635 points; the average score for pleasantness was 6.904 points; and the average score for miscellaneous odors was 3.731 points. This shows that compared with the compound essential oils in other examples and the single essential oil of the comparative example, the compound essential oil determined by the present invention has a harmonious aroma, enhances pleasantness, and the multiple essential oil blending methods weaken the miscellaneous odors of the single lavender essential oil, making the overall aroma softer.

[0058] Experiment 1 determined the most preferred compound essential oil ratio in each example. To explore the antidepressant action mode of the compound essential oil, the following experiments (Experiment 2 and Experiment 3) were carried out.

[0059] Experiment 2. Research on the antidepressant mechanism of compound essential oil based on network pharmacology

[0060] The compound essential oil studied in this experiment was the compound essential oil of Formula 4 obtained in Example 4.

[0061] In order to explore the pathways and targets of the compound essential oil in exerting antidepressant effects, the present invention explored and evaluated the targets of the compound essential oil in antidepressant treatment based on network pharmacology and speculated on the aroma components that play a major role, providing a theoretical reference for studying the antidepressant effect of aromatherapy.

[0062] To solve the above problems, the present invention provides the following technical solutions:

[0063] First, detect the aroma components in the compound essential oil and calculate their percentage contents. Use an Agilent 7890B gas chromatography system and an Agilent 7000C series triple quadrupole gas chromatography system (Agilent Technologies, Palo Alto, CA, USA) to separate and detect the main aroma components of the compound essential oil: Use a polar quartz capillary column INNOWAX (polyethylene glycol, 60 mm × 0.25 mm, film thickness 0.25 μm) and a flame ionization detector (FID) to analyze the sample. Adopt the automatic direct injection method to aspirate 0.2 μL of the mixed solution and inject it into the GC for detection. Set the temperature of the column oven to rise from the initial temperature of 50 °C to 120 °C at a rate of 3 °C / min and hold at 120 °C for 2 min, then rise to 230 °C at a rate of 5 °C / min and hold at 230 °C for 5 min. The injector temperature is 230 °C. The flow rate of the carrier gas nitrogen is 3 mL / min, and the split ratio is 1:30. Ionization is obtained by electron bombardment at 70 eV. Identify the compounds based on mass spectrometry library search (MS data recorded in NIST 20.0 and Wiley 7n.1), and calculate the percentage contents of each aroma component. The sample is subjected to 3 repeated experiments.

[0064] Speculate on the targets and action pathways of the compound essential oil in exerting antidepressant effects, as well as the aroma components that play a major role, specifically including the following steps:

[0065] S1. Screening of potential therapeutic targets of the aroma components of the compound essential oil: According to the aroma components identified in the GC-MS analysis, use the Pubchem database to obtain the SMILES numbers of the volatile aroma components through the CAS numbers, and enter this data into the SwissTargetPrediction database for collection and further screening of potential targets, and finally obtain 505 potential action targets of the compound essential oil.

[0066] S2. Collection of depression disease targets: Use the Genecards, OMIM, and CTD databases to search for disease targets related to depression. The keywords for retrieval are "depression". After unifying the formats of the targets in each database and summarizing them, screen Genecards (relevance score > 1) and remove duplicates, and finally obtain 4805 depression-related disease targets.

[0067] S3. Determination of the intersection targets of the compound essential oil in exerting antidepressant effects through biological activity

[0068] The targets were intersected using the online website Venn (https: / / bioinfogp.cnb.csic.es / tools / venny / index.html) to obtain 325 intersecting targets, which are the potential targets for the antidepressant effect of the compound essential oil. For example, Figure 2 as shown

[0069] S4. KEGG and GO enrichment of intersecting targets

[0070] To further explore the action pathway of the compound essential oil in relieving stress and depression and its effect on cells, the DAVID database (https: / / davidbioinformatics.nih.gov / ) was used to continue the KEGG and GO enrichment analysis of the intersecting targets. Figure 4 and Figure 5 are the top 10 results in the KEGG enrichment analysis and GO enrichment analysis respectively, which are the action pathways for the compound essential oil to relieve stress and depression.

[0071] S5. Screening of key aroma components

[0072] Considering that the differences in the chemical composition content in the essential oil will also affect the antidepressant effect of different components on diseases, based on the research of JiaLi et al., an evaluation coefficient for evaluating the antidepressant effect of the compound essential oil was constructed from three aspects: the content of essential oil components, potential targets, and the enriched action pathways, as follows:

[0073] (1)

[0074] In formula (1):

[0075] Ei = the effect of essential oil component i on depression

[0076] Wi = the relative content of essential oil component i in the total components of the essential oil

[0077] C ij = the number of targets of essential oil component i acting on pathway j

[0078] C j = the total number of all targets on pathway j

[0079] P j = the P value of the intersecting target enrichment pathway

[0080] By calculating the evaluation coefficients of each aroma component for its antidepressant effect, the aroma components with stronger comprehensive effects were screened as the key chemical components for the antidepressant effect of the compound essential oil. The following table shows the top ten aroma components in terms of the evaluation index of component content - target - action pathway. The components in Table 1 were identified as the key aroma components for the antidepressant effect of the compound essential oil.

[0081] Table 1

[0082]

[0083] Experiment 3: Study on the Effects of Compound Essential Oil on Electroencephalogram and Salivary Cortisol in People with Depression Tendency

[0084] Experimental Instruments and Materials: Eego mylab 56-channel amplifier (32-channel EEG, 24-channel EMG), Waveguard 32-channel shielded electrode cap CA-208 (medium size), 25-pin Trigger adapter, Eego signal acquisition software (32-channel EEG recording data acquisition module, 24-channel EMG recording module), blunt-tip syringe, conductive paste, diffuser wood, etc.

[0085] Test Subjects: The psychological status of college students was investigated throughout the school by distributing the SDS self-rating scale for emotions. According to the scale calculation model, 75 participants were selected to meet the scoring criteria for depression. Since no formal clinical diagnosis was conducted, these participants were defined as having depression tendency. Subsequently, the purpose and method of the experiment were explained to these participants in detail, and they were invited to participate in this study. Finally, 30 people participated in the EEG experiment and signed the informed consent form before the experiment. Two levels of difficulty of math problems were used to simulate different work pressure environments.

[0086] Experimental Site: A relatively quiet, clean and fixed place was selected away from external interference. The experimental light was natural and there was no strange smell, and the environmental temperature was maintained at 25°C.

[0087] Experimental Operation: The electrodes were placed on the scalp surface of the volunteers, conductive paste was injected, and all electrode impedances were controlled below 5 kΩ before the formal experiment could begin. Participants could experience emotions such as tension, uneasiness or fear when performing math tasks or learning math. Based on this theory, an experimental model that could exert pressure was established. The specific design is as follows:

[0088] Under the pressure condition, 9 addition, subtraction, multiplication and division mixed operations within three digits will be displayed on the computer screen. The participants need to calculate these results and tell the largest answer within 1 minute. Throughout the process, the participants were required to sit still on the stool and avoid any limb movements.

[0089] A blank group, a control group, experimental group 1 and experimental group 2 were set up.

[0090] Blank Group: After the subjects were ready, the diffuser wood without any treatment was placed about 10 cm below the nasal cavity of the subjects, with no pressure and no odor intervention.

[0091] Control group: After the subjects were ready, the diffuser wood without any treatment was placed about 10 cm below the nasal cavity of the subjects, and there was no odor intervention under pressure intervention.

[0092] Experimental group 1: After the subjects were ready, 3 drops of the single essential oil prepared according to the comparative example were accurately dropped on the diffuser wood, and it was placed about 10 cm below the nasal cavity of the subjects, with the influencing factor of pressure intervened.

[0093] Experimental group 2: After the subjects were ready, 3 drops of the compound essential oil prepared according to Example 4 were accurately dropped on the diffuser wood, and it was placed about 10 cm below the nasal cavity of the subjects, with the influencing factor of pressure intervened.

[0094] The participants were required to perform four groups of experiments in sequence, and the changes in the electroencephalogram of the participants during the experiments were collected simultaneously. The α wave (8 - 13 Hz) is the basic rhythm of the electroencephalogram of normal adults when awake. The higher the α wave, the more relaxed the state of the participant. The β wave (13 - 30 Hz) is most obvious in the frontal lobe and central region, and is mainly related to attention, learning, and cognitive processing, and is seen during tension, anxiety, and stress responses. In short, the increase in the β wave indicates that the participant is in a more tense state and has a higher degree of concentration. When pressure is intervened, due to the different degrees of decrease in the α wave and the different degrees of increase in the β wave, the arousal index β / α is used to comprehensively compare the changes in the electroencephalogram of the participants. The increase in its value indicates a higher degree of perceived pressure and difficulty in relaxation.

[0095] The electroencephalogram topographic map is as Figure 5 shown. Compared with the blank group, when pressure was intervened, the β / α value in the control group increased, especially in the temporal lobe region. Although the values of Experimental group 1 and Experimental group 2 also increased to varying degrees, the degree of increase was less than that of the control group. The experimental results show that both the single essential oil and the compound essential oil can relieve the tension of the participants in a stressful state and play a role in relaxation.

[0096] In order to explore the influence of aroma components on the pressure perception of electroencephalogram participants, saliva was collected, respectively set before the start of the blank group, after the end of the control group, after the end of Experimental group 1, and after the end of Experimental group 2. The collected samples were immediately placed in a -20°C refrigerator for freezing until detection.

[0097] Cortisol is a hormone related to the hypothalamic - pituitary - adrenal axis (HPA). The adaptive mechanism of the HPA axis is activated through the secretion sequence of different hormones as a response to stress. Cortisol has been widely used as a biomarker for psychological stress and mental diseases. It is widely distributed and can be detected in body parts such as human nails, hair, and saliva. The change of cortisol in saliva is in minutes, and the feedback cycle of the change level is short. The collection process is non - invasive and painless. Therefore, the measurement of salivary cortisol has become a new alternative method for stress assessment.

[0098] The cryopreserved saliva samples were thawed, centrifuged, and purified. The double antibody sandwich method was used to measure the level of human salivary cortisol in the specimens. The microplate was coated with purified human salivary cortisol antibody to prepare a solid-phase antibody. Salivary cortisol was added sequentially to the microplate coated with the monoclonal antibody, and then combined with the HRP-labeled salivary cortisol antibody to form an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, the substrate TMB was added for color development. TMB was converted into blue under the catalysis of HRP enzyme and into the final yellow under the action of acid. The intensity of the color was positively correlated with the salivary cortisol in the sample. The absorbance (OD value) was measured at a wavelength of 450 nm using an enzyme-labeled instrument, and the concentration of human salivary cortisol in the sample was calculated through the standard curve.

[0099] The experimental results are as Figure 6 shown. Compared with the blank group, when the stress factor intervened, the concentration of salivary cortisol increased significantly, demonstrating the participants' perception of stress. Compared with the control group, the concentration of salivary cortisol in the participants under the conditions of experimental group 1 and experimental group 2 decreased significantly, and the decrease amplitude in experimental group 2 was greater, and it also reached a significant level compared with experimental group 1. The data indicate that the single essential oil and compound essential oil used in this experiment can both soothe the mood and relieve stress in the participants, but the compound essential oil has a better effect.

[0100] Those skilled in the art should understand that those skilled in the art can achieve variations in combination with the prior art and the above embodiments, which will not be elaborated here. Such variations do not affect the essence of the present invention and will not be elaborated here.

[0101] The preferred embodiments of the present invention have been described above. It should be understood that the present invention is not limited to the above specific embodiments. The equipment and structures not described in detail should be understood to be implemented in a common manner in the art; any person skilled in the art, without departing from the scope of the technical solution of the present invention, can make many possible changes and modifications to the technical solution of the present invention by using the methods and technical contents disclosed above, or modify it into an equivalent embodiment with equivalent changes, which does not affect the essence of the present invention. Therefore, any simple modification, equivalent change, and modification made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention still fall within the scope of the protection of the technical solution of the present invention.

Claims

1. A pressure-relieving compound essential oil, characterized in that: It comprises the following components: lavender essential oil, Roman chamomile essential oil, peppermint essential oil, lemon balm essential oil and verbenone rosemary essential oil.

2. The anti-stress compound essential oil according to claim 1, wherein The volume ratio of the lavender essential oil, Roman chamomile essential oil, peppermint essential oil, lemon balm essential oil and verbenone rosemary essential oil is 60-100:0.01-20:0.01-10:0.01-10:0.01-15.

3. The anti-stress compound essential oil according to claim 2, characterized in that, The volume ratio of the lavender essential oil, Roman chamomile essential oil, peppermint essential oil, lemon balm essential oil and verbenone rosemary essential oil is 60:15:10:10:

5.

4. The anti-stress compound essential oil according to claim 1, wherein, The lavender essential oil is the true lavender essential oil of Space Blue.

5. The anti - stress compound essential oil according to claim 1, characterized in that, The lavender essential oil, Roman chamomile essential oil, peppermint essential oil, lemon balm essential oil and verbenone rosemary essential oil are all obtained by steam distillation.

6. A method for preparing a pressure-relieving compound essential oil according to any one of claims 1 to 5, characterized in that, The pressure-relieving compound essential oil is obtained by uniformly mixing the raw materials according to the ratio and then sealing, refrigerating and aging.

7. Application of a pressure-relieving compound essential oil according to any one of claims 1-5 in pressure-relieving products and antidepressant products.

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