Granular bio-organic fertilizer and production process thereof

Abstract

The invention relates to the technical field of fertilizers, in particular to a production process of a granular bio-organic fertilizer. Comprising the following raw materials in parts by weight: 30-50 parts of animal dung, 10-20 parts of a plant protein source, 5-10 parts of humic acid, 0.5-2 parts of a microbial agent, 5-10 parts of an aluminum-zinc chelated amino acid compound, 3-8 parts of a polypeptide compound nutrient, 5-10 parts of diatomite, 2-5 parts of a flame retardant and 0.5-1 part of sodium lignin sulfonate. According to the invention, the particles have excellent mechanical pressure resistance, so that the organic fertilizer is not easy to break in the storage process, the problem of nutrient loss caused by loose structure of the traditional organic fertilizer is avoided, the fertilizer is ensured to keep complete physical form in the long-term storage and transportation process, and a basic guarantee is provided for large-scale application; the microbial activity improvement, pollution emission reduction and soil nutrient balance are considered, and the ecological safety and sustainability of fertilizer application are realized.

Description

Technical Field

[0001] The present invention relates to the technical field of fertilizers, and specifically to a granular biological organic fertilizer and its production process. Background Art

[0002] Granular biological organic fertilizer is a highly efficient ecological fertilizer that combines organic matter and active microorganisms. It is refined through processes such as high-temperature fermentation and granulation using animal and plant residues, humic acid, amino acids, and composite functional microbial communities as the main raw materials. Its granular form facilitates mechanized application and has advantages such as slow-release nutrients, soil improvement, and enhanced crop stress resistance.

[0003] The internal structure of the existing granular biomass organic fertilizer is loose, with low mechanical strength and easy to crack under pressure, resulting in problems such as easy pulverization into powder during daily stacking storage and transportation. The pulverized granular biomass organic fertilizer not only causes dust pollution but also leads to nutrient loss, making it impossible to be widely promoted and used on a large scale.

[0004] Based on this, the present invention provides a granular biological organic fertilizer and its production process. Summary of the Invention

[0005] The purpose of the present invention is to provide a granular biological organic fertilizer and its production process to solve the problems raised in the above background art.

[0006] To achieve the above purpose, the present invention provides the following technical solution: A production process of granular biological organic fertilizer, including the following raw materials in parts by weight: 30 - 50 parts of livestock and poultry manure, 10 - 20 parts of plant protein source, 5 - 10 parts of humic acid, 0.5 - 2 parts of microbial inoculant, 5 - 10 parts of aluminum-zinc chelated amino acid complex, 3 - 8 parts of polypeptide complex nutrient, 5 - 10 parts of diatomite, 2 - 5 parts of flame retardant, and 0.5 - 1 part of sodium lignosulfonate.

[0007] Preferably, the livestock and poultry manure can be selected from one or more of chicken manure, pig manure, and cow manure, and the water content is 20 - 30% after fermentation and composting.

[0008] Preferably, the plant protein source can be selected from at least one or more of soybean meal, rapeseed meal, and cottonseed meal, and the protein content is 40 - 50%.

[0009] Preferably, the microbial inoculant is composed of Bacillus subtilis, Bacillus amyloliquefaciens, and Paenibacillus mucilaginosus. The viable count ratio of Bacillus subtilis, Bacillus amyloliquefaciens, and Paenibacillus mucilaginosus in the microbial inoculant is 1:(0.8 - 1):(0.6 - 0.8). The specific surface area of the diatomite is 15 - 20 m² / g, the average pore diameter is 10 - 20 μm, and the bulk density is 0.3 - 0.5 g / cm 3, after being calcined and pretreated at 600 - 700 °C, the porosity is ≥ 70%.

[0010] Preferably, the preparation method of the aluminum - zinc chelated amino acid complex comprises the following steps:

[0011] Step 1: Select the polarizing film of waste electronic screens, retain the PVA film with a metal coating after removing the PET substrate as the raw material, crush it to a particle size of 0.5 - 1 mm, add a hydrochloric acid solution with a mass concentration of 10 - 15%, mix according to a solid - liquid ratio of 1:(5 - 8), then stir and leach for 2 - 3 h at 60 - 80 °C and 200 - 300 r / min to extract aluminum and zinc metal ions, and then filter to remove impurities to obtain the first filtrate;

[0012] Step 2: Add a sodium hydroxide solution to the first filtrate to adjust the pH to 7 - 7.5 to obtain a mixed solution containing aluminum and zinc ions;

[0013] Step 3: Add glycine with an industrial - grade purity of ≥ 90% to the mixed solution according to a molar ratio of metal ions to added glycine of 1:(1.2 - 1.5), carry out a chelation reaction at 50 - 60 °C for 1 - 2 h, and then obtain the aluminum - zinc chelated amino acid complex after concentration and drying.

[0014] Preferably, the hydrochloric acid solution in Step 1 contains sodium dodecylbenzenesulfonate with a total mass of 0.5 - 1%.

[0015] Preferably, the preparation method of the polypeptide complex nutrient comprises the following steps:

[0016] Step 1: Collect waste human hair from barbershops as the raw material, wash and crush it to 2 - 5 mm, add deionized water according to a solid - liquid ratio of 1:(8 - 10) to adjust the pH to 9 - 10, then add alkaline protease accounting for 2 - 3% of the weight of the human hair, and carry out hydrolysis under nitrogen protection at 50 - 55 °C for 4 - 6 h to obtain a hydrolyzed polypeptide solution;

[0017] Step 2: Adjust the pH value of the hydrolyzed polypeptide solution to 5 - 5.5, then add chitosan powder accounting for 0.5 - 1% of the total mass of the hydrolyzed polypeptide solution, stir for 30 min, then put it into a centrifuge, set the rotation speed at 4000 - 5000 r / min and separate for 10 - 30 min, take the second filtrate, add a zinc sulfate solution and an iron sulfate solution with a concentration of 0.1 - 0.2 mol / L to the second filtrate for mixing, where the mass ratio of the zinc sulfate solution, the iron sulfate solution and the second filtrate is 1:1:8, and then carry out a complexation reaction at 30 - 40 °C for 1 - 2 h to obtain a complexation solution;

[0018] Step 3: Concentrate the complexation solution until the water content is ≤ 20%, and obtain the polypeptide complex nutrient after drying.

[0019] Preferably, the enzyme activity of the alkaline protease in the step 1 is ≥ 200,000 U / g.

[0020] Preferably, a production process of the granular biological organic fertilizer comprises the following steps:

[0021] S1: Pre-fermentation. Mix livestock and poultry manure, plant protein source, humic acid, diatomite, and sodium lignosulfonate, and pre-ferment at 50 - 60 °C for 3 - 5 days, controlling the water content at 50 - 60%, with the turning frequency being 1 - 2 times per day during this period, and adjusting the carbon-nitrogen ratio to (20 - 25):1 to obtain the pre-fermented material.

[0022] S2: Secondary fermentation. Add aluminum-zinc chelated amino acid complex, polypeptide complex nutrient, microbial inoculum, and flame retardant to the pre-fermented material, mix evenly, and conduct secondary fermentation. Control the temperature at 45 - 55 °C for 7 - 10 days. When the material temperature exceeds 55 °C during this period, turn and toss in time to obtain the fermented material.

[0023] S3: Granulation. Crush the fermented material to a particle size of ≤ 2 mm, then granulate it through a disk granulator, and then roll and press it through a drum screen to obtain the granular material.

[0024] S4: Low-temperature dry the granular material at 40 - 60 °C until the water content is ≤ 28%, which is the granular biological organic fertilizer.

[0025] Preferably, the preparation method of the flame retardant comprises the following steps: Weigh aluminum hydroxide powder with a particle size of ≤ 50 μm and boric acid with a purity of ≥ 99% as required, mix the aluminum hydroxide powder and boric acid according to the mass ratio of (3 - 4):1, then add deionized water according to the solid-liquid ratio of 1:(3 - 5), stir and react at 80 - 90 °C for 1 - 2 h, and obtain the granular flame retardant after spray drying.

[0026] Compared with the prior art, the beneficial effects of the present invention are:

[0027] 1. In the present invention, after high-temperature calcination, the diatomite forms a porous silica skeleton, and its three-dimensional pore network provides a physical support basis. While sodium lignosulfonate, as a natural polymer binder, forms hydrogen bonds through hydroxyl groups and silanol groups, tightly wraps the diatomite particles, and enhances the interfacial binding force. The metal ions in the aluminum-zinc chelated amino acid complex coordinate and crosslink with the carboxylic acid groups of the lignosulfonate to form a covalent bond cascade structure, further strengthening the chemical binding force inside the particles. This "rigid skeleton - flexible bonding - ionic crosslinking" composite structure enables the particles to have excellent mechanical pressure resistance, making the organic fertilizer not easy to break during storage, avoiding the nutrient loss problem caused by the loose structure of traditional organic fertilizers, ensuring the physical form of the fertilizer remains intact during long-term storage and transportation, and providing a basic guarantee for large-scale application.

[0028] 2. In the present invention, hydrolyzed human hair polypeptides serve as high-quality nutrient substrates for microorganisms. The various amino acids they contain provide essential components for the growth of the microbial community. Aluminum and zinc ions, as coenzyme factors for microbial metabolism, activate the activity of key enzymes, jointly promoting the proliferation of functional bacteria such as Bacillus subtilis, constructing a dominant microbial community, and improving the soil microecology. The porous structure of diatomite has an adsorption effect on the ammonia gas generated during the fermentation process, reducing the emission of malodorous gases and enhancing the friendliness of the production and application environment. The flame retardant adopts a compound system of aluminum hydroxide and boric acid. Its decomposition products are aluminum oxide and boric acid. The former can enhance the aggregation of soil particles, and the latter, as an essential trace element for plants, is slowly released in a safe form, overall taking into account the improvement of microbial activity, pollution reduction, and soil nutrient balance, achieving the ecological safety and sustainability of fertilizer application.

[0029] 3. In the present invention, after the fertilizer is applied to the soil, it takes effect quickly through the synergistic action of the polypeptide complex nutrient and the aluminum-zinc chelated amino acid complex. The polypeptides produced by the hydrolysis of human hair contain abundant amino and carboxyl groups, serving as high-efficiency carbon and nitrogen sources for microorganisms, which can be directly recognized and utilized by soil functional bacteria, inducing them to secrete degradation enzymes such as protease and cellulase, accelerating the decomposition of macromolecular organic matter in livestock and poultry manure and plant protein sources. The metal ions dissociated from the aluminum-zinc chelated amino acid complex in the soil promote the colonization and proliferation of functional microbial communities such as phosphate-solubilizing bacteria and nitrogen-fixing bacteria by regulating the pH value of the microenvironment and participating in microbial metabolism. The coordination between the polar groups of the polypeptides and the metal ions jointly provides a suitable growth environment for microorganisms, forming a benign metabolic cycle, enabling the organic matter and nutrients in the organic fertilizer to be quickly converted into a form that can be absorbed by crops, shortening the nutrient release period, meeting the nutritional needs of crops during the critical growth period, and achieving the efficient exertion of fertilizer efficacy. Specific Embodiments

[0030] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0031] Example 1. This example provides a production process for granular biological organic fertilizer, including the following raw materials in parts by weight: 30 parts of livestock and poultry manure, 10 parts of plant protein source, 5 parts of humic acid, 0.5 part of microbial inoculant, 5 parts of aluminum-zinc chelated amino acid complex, 3 parts of polypeptide complex nutrient, 5 parts of diatomite, 2 parts of flame retardant, and 0.5 part of lignosulfonate.

[0032] Among them, the livestock and poultry manure is selected as chicken manure, and the moisture content is 20% after fermentation and composting.

[0033] Among them, the plant protein source is soybean meal with a protein content of 40%.

[0034] Among them, the microbial inoculant is composed of Bacillus subtilis, Bacillus amyloliquefaciens, and Paenibacillus mucilaginosus. The viable count ratio of Bacillus subtilis, Bacillus amyloliquefaciens, and Paenibacillus mucilaginosus in the microbial inoculant is 1:0.8:0.6. The specific surface area of diatomite is 15 m² / g, the average pore diameter is 10 μm, and the bulk density is 0.3 g / cm 3 , after being pretreated by calcination at 600 °C, the porosity is ≥70%.

[0035] Among them, the preparation method of the aluminum-zinc chelated amino acid complex includes the following steps:

[0036] Step 1: Select the polarizing film of the waste electronic screen. After removing the PET substrate, retain the PVA film with a metal coating as the raw material, crush it to a particle size of 0.5 mm, add a hydrochloric acid solution with a mass concentration of 10%, mix according to a solid-liquid ratio of 1:5, and then stir and acid-leach for 2 h at 60 °C and 200 r / min to extract aluminum and zinc metal ions. Then filter to remove impurities to obtain the first filtrate;

[0037] Step 2: Add a sodium hydroxide solution to the first filtrate to adjust the pH to 7 to obtain a mixed solution containing aluminum and zinc ions;

[0038] Step 3: According to the molar ratio of metal ions to glycine added of 1:1.2, add glycine with an industrial purity of ≥90% to the mixed solution, carry out a chelation reaction at 50 °C for 1 h, and then obtain the aluminum-zinc chelated amino acid complex after concentration and drying.

[0039] Among them, the hydrochloric acid solution in Step 1 contains sodium dodecylbenzenesulfonate with a total mass of 0.5%.

[0040] Among them, the preparation method of the polypeptide complex nutrient includes the following steps:

[0041] Step 1: Collect waste human hair from a barbershop as the raw material. After cleaning, crush it to 2 mm, adjust the pH to 9 by adding deionized water according to a solid-liquid ratio of 1:8, and then add alkaline protease accounting for 2% of the weight of the human hair. Carry out hydrolysis for 4 h under nitrogen protection at 50 °C to obtain a hydrolyzed polypeptide solution;

[0042] Step 2: Adjust the pH value of the hydrolyzed polypeptide solution to 5, then add chitosan powder accounting for 0.5% of the total mass of the hydrolyzed polypeptide solution, stir for 30 min, and then put it into a centrifuge. Set the rotation speed to 4000 r / min and separate for 10 min to obtain the second filtrate. Add a zinc sulfate solution with a concentration of 0.1 mol / L and an iron sulfate solution to the second filtrate for mixing. The mass ratio of the zinc sulfate solution, the iron sulfate solution, and the second filtrate is 1:1:8, and then carry out a complexation reaction at 30 °C for 1 h to obtain a complex solution;

[0043] Step 3: Concentrate the complex solution until the water content is ≤ 20%, and then dry it to obtain the polypeptide complex nutrient.

[0044] Among them, in Step 1, the enzyme activity of the alkaline protease is ≥ 200,000 U / g.

[0045] Among them, a production process of a granular biological organic fertilizer includes the following steps:

[0046] S1: Pre-fermentation. Mix livestock and poultry manure, plant protein source, humic acid, diatomite, and sodium lignosulfonate, and pre-ferment at 50 °C for 3 days, controlling the water content at 50%. During this period, the turning frequency is once a day, and adjust the carbon-nitrogen ratio to 20:1 to obtain the pre-fermented material.

[0047] S2: Secondary fermentation. Add aluminum-zinc chelated amino acid complex, polypeptide complex nutrient, microbial inoculum, and flame retardant to the pre-fermented material, mix evenly, and conduct secondary fermentation. Control the temperature at 45 °C for 7 days. When the material temperature exceeds 55 °C during this period, turn it over in time to obtain the fermented material.

[0048] S3: Granulation. Crush the fermented material to a particle size of ≤ 2 mm, then granulate it through a disk granulator, and then roll it through a drum screen to obtain the granular material.

[0049] S4: Low-temperature dry the granular material at 40 °C until the moisture content is ≤ 28%, which is the granular biological organic fertilizer.

[0050] Among them, the preparation method of the flame retardant includes the following steps: Weigh aluminum hydroxide powder with a particle size of ≤ 50 μm and boric acid with a purity of ≥ 99% as required. Mix the aluminum hydroxide powder and boric acid in a mass ratio of 3:1, then add deionized water according to a solid-liquid ratio of 1:3, stir and react at 80 °C for 1 h, and obtain the granular flame retardant after spray drying.

[0051] Example 2. A production process of a granular biological organic fertilizer includes the following raw materials in parts by weight: 50 parts of livestock and poultry manure, 20 parts of plant protein source, 10 parts of humic acid, 2 parts of microbial inoculum, 10 parts of aluminum-zinc chelated amino acid complex, 8 parts of polypeptide complex nutrient, 10 parts of diatomite, 5 parts of flame retardant, and 1 part of sodium lignosulfonate.

[0052] Among them, the livestock and poultry manure is selected as pig manure, and the moisture content after fermentation and decomposition is 30%.

[0053] Among them, the plant protein source is selected as cottonseed meal, and the protein content is 50%.

[0054] Among them, the microbial inoculant is composed of Bacillus subtilis, Bacillus amyloliquefaciens, and Paenibacillus mucilaginosus. The viable count ratio of Bacillus subtilis, Bacillus amyloliquefaciens, and Paenibacillus mucilaginosus in the microbial inoculant is 1:1:0.8. The specific surface area of diatomite is 20 ㎡ / g, the average pore diameter is 20 μm, and the bulk density is 0.5 g / cm 3 , after being pretreated by calcination at 700 °C, the porosity is ≥70%.

[0055] Among them, the preparation method of the aluminum-zinc chelated amino acid complex includes the following steps:

[0056] Step 1: Select the polarizing film of the waste electronic screen, remove the PET substrate and retain the PVA film with a metal coating as the raw material. Crush it to a particle size of 1 mm, add a hydrochloric acid solution with a mass concentration of 15%, mix according to a solid-liquid ratio of 1:8, and then stir and acid-leach for 3 h at 80 °C and 300 r / min to extract aluminum and zinc metal ions. Then filter to remove impurities to obtain the first filtrate;

[0057] Step 2: Add a sodium hydroxide solution to the first filtrate to adjust the pH to 7.5 to obtain a mixed solution containing aluminum and zinc ions;

[0058] Step 3: Add glycine with an industrial purity of ≥90% to the mixed solution according to a molar ratio of metal ions to glycine of 1:1.5, and carry out a chelation reaction at 60 °C for 2 h. Then, after concentration and drying, an aluminum-zinc chelated amino acid complex is prepared.

[0059] Among them, the hydrochloric acid solution in Step 1 contains 1% by total mass of sodium dodecylbenzenesulfonate.

[0060] Among them, the preparation method of the polypeptide complex nutrient includes the following steps:

[0061] Step 1: Collect waste human hair from a barbershop as the raw material. After washing, crush it to 5 mm, add deionized water according to a solid-liquid ratio of 1:10 to adjust the pH to 10, and then add alkaline protease at 3% of the weight of the human hair. Carry out hydrolysis under nitrogen protection at 55 °C for 6 h to obtain a hydrolyzed polypeptide solution;

[0062] Step 2: Adjust the pH value of the hydrolyzed polypeptide solution to 5.5, then add chitosan powder at 1% of the total mass of the hydrolyzed polypeptide solution, stir for 30 min, and then put it into a centrifuge. Set the rotation speed at 5000 r / min and separate for 30 min to obtain the second filtrate. Add a zinc sulfate solution and an iron sulfate solution with a concentration of 0.2 mol / L to the second filtrate and mix them. The mass ratio of the zinc sulfate solution, the iron sulfate solution, and the second filtrate is 1:1:8. Then carry out a complexation reaction at 40 °C for 2 h to obtain a complex solution;

[0063] Step 3: Concentrate the complex solution until the water content is ≤20%, and dry it to obtain the polypeptide complex nutrient.

[0064] Among them, the enzyme activity of the alkaline protease in Step 1 is ≥ 200,000 U / g.

[0065] Among them, a production process of granular biological organic fertilizer includes the following steps:

[0066] S1: Pre-fermentation. Mix livestock and poultry manure, plant protein source, humic acid, diatomite, and sodium lignosulfonate, and pre-ferment at 60 °C for 5 days, controlling the water content at 60%, with the turning frequency being 2 times a day during this period, and adjusting the carbon-nitrogen ratio to 25:1 to obtain the pre-fermented material.

[0067] S2: Secondary fermentation. Add aluminum-zinc chelated amino acid complex, polypeptide complex nutrient, microbial inoculum, and flame retardant to the pre-fermented material, mix evenly, and carry out secondary fermentation at a temperature controlled at 55 °C for 10 days. When the material temperature exceeds 55 °C during this period, turn it over in time to obtain the fermented material.

[0068] S3: Granulation. Crush the fermented material to a particle size of ≤ 2 mm, then granulate it through a disk granulator, and then roll it through a drum screen to obtain granular material.

[0069] S4: Low-temperature dry the granular material at 60 °C until the moisture content is ≤ 28%, which is the granular biological organic fertilizer.

[0070] Among them, the preparation method of the flame retardant includes the following steps: Weigh aluminum hydroxide powder with a particle size of ≤ 50 μm and boric acid with a purity of ≥ 99% as required. Mix the aluminum hydroxide powder and boric acid in a mass ratio of 4:1, then add deionized water according to a solid-liquid ratio of 1:5, stir and react at 90 °C for 2 h, and obtain granular flame retardant after spray drying.

[0071] Example 3. A production process of granular biological organic fertilizer includes the following raw materials in parts by weight: 40 parts of livestock and poultry manure, 15 parts of plant protein source, 8 parts of humic acid, 1 part of microbial inoculum, 8 parts of aluminum-zinc chelated amino acid complex, 5 parts of polypeptide complex nutrient, 8 parts of diatomite, 4 parts of flame retardant, and 0.8 part of sodium lignosulfonate.

[0072] Among them, the livestock and poultry manure is selected as pig manure, and the moisture content is 25% after fermentation and decomposition.

[0073] Among them, the plant protein source is selected as rapeseed meal, and the protein content is 45%.

[0074] Among them, the microbial inoculum is composed of Bacillus subtilis, Bacillus amyloliquefaciens, and Paenibacillus mucilaginosus. The viable bacteria number ratio of Bacillus subtilis, Bacillus amyloliquefaciens, and Paenibacillus mucilaginosus in the microbial inoculum is 1:0.9:0.7. The specific surface area of diatomite is 18 ㎡ / g, the average pore diameter is 15 μm, and the bulk density is 0.4 g / cm 3, after being calcined and pretreated at 650 °C, the porosity is ≥ 70%.

[0075] Among them, the preparation method of the aluminum-zinc chelated amino acid complex includes the following steps:

[0076] Step 1: Select the polarizing film of the waste electronic screen, retain the PVA film with a metal coating after removing the PET substrate as the raw material, crush it to a particle size of 0.8 mm, add a hydrochloric acid solution with a mass concentration of 13%, mix according to a solid-liquid ratio of 1:6, and then stir and acid-leach at 70 °C and 250 r / min for 2.5 h to extract aluminum and zinc metal ions, and then filter to remove impurities to obtain the first filtrate;

[0077] Step 2: Add a sodium hydroxide solution to the first filtrate to adjust the pH to 7.3 to obtain a mixed solution containing aluminum and zinc ions;

[0078] Step 3: Add glycine with an industrial purity of ≥ 90% to the mixed solution according to a molar ratio of metal ions to added glycine of 1:1.4, carry out a chelation reaction at 55 °C for 1.5 h, and then obtain the aluminum-zinc chelated amino acid complex after concentration and drying.

[0079] Among them, the hydrochloric acid solution in Step 1 contains sodium dodecylbenzenesulfonate with a total mass of 0.8%.

[0080] Among them, the preparation method of the polypeptide complex nutrient includes the following steps:

[0081] Step 1: Collect waste human hair from barbershops as the raw material, wash and crush it to 4 mm, add deionized water according to a solid-liquid ratio of 1:9 to adjust the pH to 9.5, and then add alkaline protease accounting for 2.5% of the weight of the human hair, and carry out hydrolysis under nitrogen protection at 53 °C for 5 h to obtain a hydrolyzed polypeptide solution;

[0082] Step 2: Adjust the pH value of the hydrolyzed polypeptide solution to 5.3, add chitosan powder accounting for 0.8% of the total mass of the hydrolyzed polypeptide solution, stir for 30 min, then put it into a centrifuge, set the rotation speed at 4500 r / min and separate for 20 min to obtain the second filtrate. Add a zinc sulfate solution with a concentration of 0.15 mol / L and an iron sulfate solution to the second filtrate for mixing, where the mass ratio of the zinc sulfate solution, the iron sulfate solution and the second filtrate is 1:1:8, and then carry out a complexation reaction at 35 °C for 1.5 h to obtain a complex solution;

[0083] Step 3: Concentrate the complex solution until the water content is ≤ 20%, and dry it to obtain the polypeptide complex nutrient.

[0084] Among them, the enzyme activity of the alkaline protease in Step 1 is ≥ 200,000 U / g.

[0085] Among them, a production process of a granular biological organic fertilizer includes the following steps:

[0086] S1: Pre-fermentation. Mix livestock and poultry manure, plant protein source, humic acid, diatomite, and sodium lignosulfonate, and perform pre-fermentation at 55°C for 4 days, controlling the water content at 55%. During this period, the turning frequency is 1.5 times per day, and the carbon-nitrogen ratio is adjusted to 23:1 to obtain the pre-fermented material.

[0087] S2: Secondary fermentation. Add aluminum-zinc chelated amino acid complex, polypeptide complex nutrient, microbial inoculant, and flame retardant to the pre-fermented material, mix evenly, and perform secondary fermentation at a temperature controlled at 50°C for 8 days. When the material temperature exceeds 55°C during this period, turn and toss in time to obtain the fermented material.

[0088] S3: Granulation. Crush the fermented material to a particle size of ≤2 mm, then granulate it through a disc granulator, and finally roll and press it through a drum sieve to obtain the granular material.

[0089] S4: Low-temperature dry the granular material at 50°C until the moisture content is ≤28%, which is the granular bio-organic fertilizer.

[0090] Among them, the preparation method of the flame retardant includes the following steps: Weigh aluminum hydroxide powder with a particle size of ≤50 μm and boric acid with a purity of ≥99% as required, mix the aluminum hydroxide powder and boric acid according to a mass ratio of 3.5:1, then add deionized water according to a solid-liquid ratio of 1:4, stir and react at 85°C for 1.5 h, and obtain the granular flame retardant after spray drying.

[0091] Comparative Example 1. The difference between this comparative example and Examples 1-3 is that: in the production process of the granular bio-organic fertilizer in this comparative example, the aluminum-zinc chelated amino acid complex is not added.

[0092] Comparative Example 2. The difference between this comparative example and Examples 1-3 is that: in the production process of the granular bio-organic fertilizer in this comparative example, the polypeptide complex nutrient is not added.

[0093] Comparative Example 3. The difference between this comparative example and Examples 1-3 is that: in the production process of the granular bio-organic fertilizer in this comparative example, neither the aluminum-zinc chelated amino acid complex nor the polypeptide complex nutrient is added.

[0094] Performance test:

[0095] Storage compressive capacity test: Randomly select 10 dry granules, place each granule under the probe of the granule strength tester one by one, apply pressure evenly until the granule breaks, record the peak pressure, and take the average value.

[0096] Soil decomposition rate test: Weigh 5 g of the particles and place them in a nylon mesh bag with a pore size of 0.25 mm. Bury the bag in loam soil with a water content of 60% (pH 7.0, organic matter content 2%) and incubate at a constant temperature of 50 °C for 7 days. Take out the mesh bag, rinse the residual soil with deionized water, and dry it to a constant weight at 60 °C. Calculate the weight loss rate (decomposition rate = (initial weight - residual weight) / initial weight × 100%);

[0097] Determination of viable microbial count: Using the dilution coating plate method, dilute the fertilizer sample by 10 5 times, coat it on the beef extract peptone medium, and count the number of colonies after culturing at 37 °C for 48 hours, and convert the viable count (CFU / g).

[0098] Performance tests were carried out on the granular bio-organic fertilizers produced in Examples 1-3 and Comparative Examples 1-3, and the test data obtained were recorded in the following table:

[0099]

[0100] From the comparison of the data in the table, it can be seen that the compressive strength test results in Examples 1-3 are significantly better than those in Comparative Examples 1 and 3. This is because during the production process of the granular bio-organic fertilizer, the diatomite forms a porous silica skeleton after high-temperature calcination, and its three-dimensional pore network provides a physical support basis. While sodium lignosulfonate, as a natural polymer binder, forms hydrogen bonds through hydroxyl groups and silanol groups to tightly wrap the diatomite particles, enhancing the interfacial binding force. The metal ions in the aluminum-zinc chelated amino acid complex coordinate and crosslink with the carboxylic acid groups of the lignosulfonate to form a covalent bond cascade structure, further strengthening the chemical binding force inside the particles. This "rigid skeleton - flexible bonding - ionic crosslinking" composite structure endows the particles with excellent anti-mechanical pressure performance, making the organic fertilizer not easily broken during storage, avoiding the nutrient loss problem caused by the loose structure of traditional organic fertilizers, ensuring the physical integrity of the fertilizer during long-term storage and transportation, and providing a basic guarantee for large-scale application;

[0101] It can be seen from the comparison of the data in the table that the test results of the 7-day decomposition rate of the soil in Examples 1-3 are significantly better than those in Comparative Example 2 and Comparative Example 3. This is because human hair hydrolyzed polypeptide, as a high-quality nutrient matrix for microorganisms, contains various amino acids that provide essential components for the growth of the microbial community. And aluminum and zinc ions, as coenzyme factors for microbial metabolism, activate the activity of key enzymes, jointly promote the proliferation of functional bacteria such as Bacillus subtilis, construct a dominant microbial community, improve the soil microecology. The porous structure of diatomite has an adsorption effect on the ammonia gas generated during the fermentation process, reduces the emission of malodorous gases, and improves the friendliness of the production and application environment. The flame retardant adopts a compound system of aluminum hydroxide and boric acid, and its decomposition products are aluminum oxide and boric acid. The former can enhance the aggregation of soil particles, and the latter, as an essential trace element for plants, is slowly released in a safe form, overall taking into account the improvement of microbial activity, pollution reduction and soil nutrient balance, and realizing the ecological safety and sustainability of fertilizer application;

[0102] It can be seen from the comparison of the data in the table that the test results of the viable bacteria count in Examples 1-3 are significantly better than those in Comparative Example 3. This is because after the fertilizer is applied to the soil, it takes effect quickly through the synergistic effect of the polypeptide complex nutrient and the aluminum-zinc chelated amino acid complex. The polypeptide produced by the hydrolysis of human hair contains abundant amino and carboxyl groups, as a high-efficiency carbon and nitrogen source for microorganisms, which can be directly recognized and utilized by soil functional bacteria, inducing them to secrete degradation enzymes such as protease and cellulase, accelerating the decomposition of macromolecular organic matter in livestock manure and plant protein sources. The Al 3+ 、Zn 2+ ions dissociated from the aluminum-zinc chelated amino acid complex in the soil promote the colonization and proliferation of functional bacterial communities such as phosphate-solubilizing bacteria and nitrogen-fixing bacteria by regulating the pH value of the microenvironment and participating in microbial metabolism. The coordination effect between the polar groups of the polypeptide and the metal ions jointly provides a suitable growth environment for microorganisms, forming a benign metabolic cycle, enabling the organic matter and nutrients in the organic fertilizer to be quickly converted into a form that can be absorbed by crops, shortening the nutrient release period, meeting the nutrient requirements of crops during the critical growth period, and realizing the efficient exertion of fertilizer efficiency.

[0103] In the description of this specification, the descriptions referring to terms such as "one embodiment", "example", "specific example", etc. mean that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in a suitable manner in any one or more embodiments or examples.

[0104] The preferred embodiments of the present invention disclosed above are only used to help illustrate the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to the specific embodiments described. Obviously, many modifications and variations can be made according to the content of this specification. These embodiments are selected and specifically described in this specification to better explain the principles and practical applications of the present invention, so that those skilled in the art can understand and utilize the present invention well. The present invention is only limited by the claims and their full scope and equivalents.

Claims

1. A production process of granular biological organic fertilizer, characterized in that, It includes the following raw materials in parts by weight: 30-50 parts of livestock and poultry manure, 10-20 parts of plant protein source, 5-10 parts of humic acid, 0.5-2 parts of microbial inoculum, 5-10 parts of aluminum-zinc chelated amino acid complex, 3-8 parts of polypeptide complex nutrient, 5-10 parts of diatomite, 2-5 parts of flame retardant, and 0.5-1 part of sodium lignosulfonate.

2. The production process of the granular biological organic fertilizer according to claim 1, characterized in that The livestock and poultry manure can be selected from one or more of chicken manure, pig manure, and cow manure, and after fermentation and composting, the moisture content is 20-30%.

3. The production process of the granular biological organic fertilizer according to claim 1, characterized in that, The plant protein source can be selected from at least one or a mixture of soybean meal, rapeseed meal, and cottonseed meal, and the protein content is 40-50%.

4. The production process of the granular biological organic fertilizer according to claim 1, wherein, The microbial inoculant is composed of Bacillus subtilis, Bacillus amyloliquefaciens, and Paenibacillus mucilaginosus. The viable count ratio of Bacillus subtilis, Bacillus amyloliquefaciens, and Paenibacillus mucilaginosus in the microbial inoculant is 1:(0.8 - 1):(0.6 - 0.8). The specific surface area of the diatomite is 15 - 20 m² / g, the average pore diameter is 10 - 20 μm, and the bulk density is 0.3 - 0.5 g / cm 3 , after being calcined and pretreated at 600 - 700 °C, the porosity is ≥70%.

5. The production process of the granular biological organic fertilizer according to claim 1, characterized in that, The preparation method of the aluminum-zinc chelated amino acid complex includes the following steps: Step 1: Select the polarizing film of the waste electronic screen, remove the PET substrate and retain the PVA film with a metal coating as the raw material, crush it to a particle size of 0.5-1 mm, add a hydrochloric acid solution with a mass concentration of 10-15%, mix according to a solid-liquid ratio of 1:(5-8), and then stir and acid-leach at 60-80 °C and 200-300 r / min for 2-3 h to extract aluminum and zinc metal ions, and then filter to remove impurities to obtain the first filtrate; Step 2: Add a sodium hydroxide solution to the first filtrate to adjust the pH to 7-7.5 to obtain a mixed solution containing aluminum and zinc ions; Step 3: According to the molar ratio of metal ions to glycine added of 1:(1.2-1.5), add glycine with an industrial purity of ≥90% to the mixed solution, and carry out a chelation reaction at 50-60 °C for 1-2 h, and then obtain the aluminum-zinc chelated amino acid complex after concentration and drying.

6. The production process of the granular biological organic fertilizer according to claim 5, characterized in that, In the hydrochloric acid solution in Step 1, sodium dodecylbenzenesulfonate with a total mass of 0.5-1% is contained.

7. The production process of the granular biological organic fertilizer according to claim 1, characterized in that, The preparation method of the polypeptide complex nutrient includes the following steps: Step 1: Collect waste human hair from a barbershop as the raw material, wash it and crush it to 2-5 mm, add deionized water according to a solid-liquid ratio of 1:(8-10) to adjust the pH to 9-10, and then add alkaline protease accounting for 2-3% of the weight of the human hair, and carry out hydrolysis under nitrogen protection at 50-55 °C for 4-6 h to obtain a hydrolyzed polypeptide solution; Step 2: Adjust the pH value of the hydrolyzed polypeptide solution to 5-5.5, then add chitosan powder accounting for 0.5-1% of the total mass of the hydrolyzed polypeptide solution, stir for 30 min, then put it into a centrifuge, set the rotation speed at 4000-5000 r / min and separate for 10-30 min to obtain the second filtrate. Add a zinc sulfate solution and a ferrous sulfate solution with a concentration of 0.1-0.2 mol / L to the second filtrate and mix them. The mass ratio of the zinc sulfate solution, the ferrous sulfate solution and the second filtrate is 1:1:8, and then carry out a complexation reaction at 30-40 °C for 1-2 h to obtain a complex solution; Step 3: Concentrate the complex solution until the water content ≤20%, and dry it to obtain the polypeptide complex nutrient.

8. The production process of the granular biological organic fertilizer according to claim 7, characterized in that In Step 1, the enzyme activity of the alkaline protease ≥200,000 U / g.

9. The production process of the granular biological organic fertilizer according to any one of claims 1 to 8, characterized in that, It includes the following steps: S1: Pre-fermentation. Mix livestock and poultry manure, plant protein source, humic acid, diatomite, and sodium lignosulfonate, and conduct pre-fermentation at 50 - 60 °C for 3 - 5 days, controlling the water content at 50 - 60%, with the turning frequency being 1 - 2 times per day during this period, and adjusting the carbon-nitrogen ratio to (20 - 25):1 to obtain the pre-fermented material. S2: Secondary fermentation. Add aluminum-zinc chelated amino acid complex, polypeptide complex nutrient, microbial inoculant, and flame retardant to the pre-fermented material, mix evenly, and conduct secondary fermentation. Control the temperature at 45 - 55 °C for 7 - 10 days. When the material temperature exceeds 55 °C during this period, turn and toss in time to obtain the fermented material. S3: Granulation. Crush the fermented material to a particle size of ≤2 mm, then granulate it through a disk granulator, and then roll and press it through a drum sieve to obtain granular material. S4: Low-temperature dry the granular material at 40 - 60 °C until the moisture content is ≤28%, which is the granular biological organic fertilizer.

10. The production process of the granular biological organic fertilizer according to claim 1, characterized in that, The preparation method of the flame retardant includes the following steps: Weigh aluminum hydroxide powder with a particle size of ≤50 μm and boric acid with a purity of ≥99% as required. Mix the aluminum hydroxide powder and boric acid in a mass ratio of (3 - 4):1, then add deionized water according to a solid-liquid ratio of 1:(3 - 5), stir and react at 80 - 90 °C for 1 - 2 h, and obtain granular flame retardant after spray drying.

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