Repair synergistic composition applicable to non-traumatic medical beauty and application thereof

Abstract

The invention relates to a repairing synergistic composition applicable to non-traumatic medical beauty and application of the repairing synergistic composition. The composition comprises the following components: oat beta-glucan, an oat kernel extract, a holy basil leaf extract, a hop extract and a Brazil fruit seed extract, the weight ratio of the oat beta glucan to the oat kernel extract to the holy basil leaf extract to the humulus lupulus extract to the Brazil fruit seed extract is (0.11-3): (0.1-2): (1-5): (0.1-1): (0.2-2). According to the composition disclosed by the invention, through a multi-dimensional synergistic effect mechanism, the skin barrier is strengthened before an operation, the tolerance is increased, the effect is enhanced through postoperative repair, the color sinking and acne formation are prevented, and the complete-cycle closed-loop management of preoperative defense-postoperative repair-effect maintenance of non-traumatic medical beauty is realized.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a repairing and enhancing composition suitable for non-invasive medical aesthetics and an application thereof. Background Art

[0002] Medical cosmetology (medical aesthetics) is a method of improving appearance and body shape through medical technology, which is divided into two categories: traumatic and non-traumatic. Non-traumatic medical aesthetics (light medical aesthetics) include photorejuvenation, radiofrequency lifting, injection of water light needles, Thermage, etc., which mainly act on the surface or dermis of the skin through light, electricity, heat or injection, without surgical incision, and short recovery period. Traumatic medical aesthetics such as fractional laser, microneedle, thread carving, etc., cause skin damage through minimally invasive or surgical methods to promote collagen regeneration. The recovery period is long and requires special care. General skin care products cannot be used. Although non-traumatic projects have no open wounds, the photothermal effect may damage the skin barrier function, causing dryness, redness or photosensitive pigmentation. For example, photorejuvenation will reduce the skin's ability to retain moisture, and the barrier needs to be repaired through postoperative hydration; radiofrequency treatment may cause a transient inflammatory response and require anti-inflammatory care. Pre-medical aesthetic care can ensure that skin tolerance is improved and reduce the risk of intraoperative irritation. Medical aesthetics stimulates skin regeneration through the “break first, then build” mechanism. Postoperative repair can prolong the therapeutic effect and prevent complications such as acne breakouts caused by flora disorders, post-inflammatory pigmentation, and sensitive skin irritation.

[0003] The current development of post-operative repair products for medical aesthetics focuses on passive repair in a single postoperative stage, such as using a combination of hyaluronic acid, collagen or repairing plant extracts to relieve postoperative redness, dryness and other problems through moisturizing or anti-inflammatory effects. However, such products generally have the following defects: First, the existing technology lacks active reinforcement of the preoperative barrier function, which makes the skin prone to acute inflammatory reactions due to thermal effects or mechanical stimulation during medical aesthetic operations, affecting the efficiency of postoperative recovery; second, most products only moisturize the epidermis or anti-inflammatory the superficial layer, and the ingredients are not synergistic enough, and they fail to simultaneously regulate the collagen metabolism, microecological balance and cell connection structure of the dermis, resulting in secondary problems such as repeated pigmentation and acne outbreaks after surgery; third, traditional formulas do not provide care for pre-operative barrier strengthening of medical aesthetics, and do not take into account that establishing skin tolerance in advance is also an important task to increase medical aesthetic effects and reduce medical aesthetic stimulation. Summary of the invention

[0004] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a non-invasive repair and synergistic composition suitable for medical aesthetics and its application.

[0005] To achieve the above object, the technical solution adopted by the present invention is:

[0006] In a first aspect, the present invention provides a repair and synergistic composition applicable to non-invasive medical aesthetics, comprising the following components: oat β-glucan, oat kernel extract, holy basil leaf extract, hops extract and brazil nut seed extract, and the weight ratio of the oat β-glucan, oat kernel extract, holy basil leaf extract, hops extract and brazil nut seed extract is (0.11-3):(0.1-2):(1-5):(0.1-1):(0.2-2).

[0007] The composition of the present invention achieves full-cycle care for non-invasive medical aesthetics through multi-dimensional synergistic effects, specifically as follows:

[0008] Oat β-glucan forms a dynamic protective film in the epidermis layer with its unique glycosidic bond structure, promotes stratum corneum regeneration and dermal collagen synthesis by activating cell signaling pathways, enhances the skin's ability to resist external stimuli, and simultaneously constructs a biomimetic lipid network synergistically with oat kernel extract to optimize the mechanical strength and water-locking function of the barrier.

[0009] The oat kernel extract reduces the release of key inflammatory mediators by inhibiting the inflammatory signal transduction pathway. Its natural lipid components and oat β-glucan molecules form a three-dimensional barrier structure through hydrophobic interaction, further consolidating the integrity of the stratum corneum, and inhibiting the proliferation of pathogenic bacteria at the microecological level, reducing the risk of postoperative adverse reactions.

[0010] The holy basil leaf extract inhibits the activity of tyrosinase and the MITF pathway through active ingredients (ursolic acid, eugenol, etc.), directly reducing melanin production; simultaneously blocking the inflammatory pathway and scavenging free radicals to avoid the activation of melanocytes by inflammation and oxidative stress. In addition, its property of promoting the expression of barrier proteins can synergistically strengthen the physical defense of the skin with oat β-glucan, reducing the risk of pigmentation induced by external stimuli.

[0011] The hops extract reduces the excessive release of inflammatory factors by targeting and regulating the balance of skin surface flora and the activity of nerve receptors, and simultaneously strengthens the expression of intercellular junction proteins in keratinocytes, forming a dual protection of microbial barrier and physical barrier, significantly reducing the occurrence probability of secondary problems such as postoperative acne.

[0012] The brazil nut seed extract promotes the secretion of epidermal protective lipids by activating the lipid synthesis-related pathway, accelerates the differentiation and maturation process of keratinocytes, and simultaneously enhances the stability of cytoskeletal proteins and connection structures, improving the mechanical strength of tight junctions in the epidermis layer, forming a closed-loop repair system from cell regeneration to barrier strengthening.

[0013] In the composition of the present invention, each component acts through a phased mechanism, constructing a defensive barrier before surgery to increase skin tolerance, preventing strong irritation reactions during surgery, promoting synergistic regeneration after surgery, enhancing the aesthetic medical effect, preventing inflammatory pigmentation and acne breakouts, and forming a systematic solution covering the entire cycle of aesthetic medicine.

[0014] Preferably, the weight ratio of the oat β-glucan, oat kernel extract, holy basil leaf extract, hops extract, and brazil nut seed extract is (0.5 - 2):(1 - 2):(1 - 3):(0.1 - 1.2):(0.5 - 1.2).

[0015] Preferably, the weight ratio of the oat β-glucan, oat kernel extract, holy basil leaf extract, hops extract, and brazil nut seed extract is (1 - 1.2):(1.5 - 2):(1 - 2):(0.1 - 0.8):(0.8 - 1).

[0016] In a second aspect, the present invention provides the use of the repair and synergistic enhancement composition applicable to non-invasive aesthetic medicine described in the first aspect in the preparation of cosmetics.

[0017] Preferably, the cosmetics are lotion, emulsion, cream, mask, essence, or spray, and the addition amount of the composition is 1% - 5% of the total weight of the cosmetics.

[0018] In a third aspect, the present invention provides an essence, comprising raw materials in the following weight percentages: 1% - 5% of the repair and synergistic enhancement composition applicable to non-invasive aesthetic medicine described in the first aspect, 0.05% - 0.5% thickener, 3% - 10% humectant, 0.5% - 3% preservative, and 0.01% - 0.3% pH regulator, with the balance being deionized water.

[0019] Preferably, the thickener includes at least one of xanthan gum, carbomer, acryloyldimethyltaurate / VP copolymer, ammonium acryloyldimethyltaurate / VP copolymer, and sclerotium gum.

[0020] Preferably, the humectant includes at least one of allantoin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, D-panthenol, sodium hyaluronate, 1,2-butanediol, glycerol, pullulan, and ceramide.

[0021] Preferably, the pH regulator includes at least one of arginine, tromethamine, and disodium EDTA.

[0022] Preferably, the preservative includes at least one of 1,3-propanediol, 1,2-hexanediol, and p-hydroxyacetophenone.

[0023] Fourthly, the present invention provides a method for preparing the essence in the third aspect, comprising the following steps:

[0024] S1. Mix a humectant, a thickener and part of deionized water, homogenize at 75 - 85 °C, then add a preservative and stir evenly to obtain a mixture;

[0025] S2. Wait until the temperature of the mixture in S1 drops to 35 - 45 °C, add each component of the repair and enhancement composition applicable to non-invasive medical aesthetics and the remaining deionized water, stir evenly, and finally add a pH regulator to adjust the pH to obtain the essence.

[0026] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0027] The components of the repair and enhancement composition applicable to non-invasive medical aesthetics of the present invention are oat β-glucan, oat kernel extract, holy basil leaf extract, hops extract and brazil nut seed extract, which can synergistically promote collagen production, enhance dermal toughness, improve barrier defense and tolerance before surgery to resist damage, repair skin damage after surgery, reduce irritation sensitivity and promote wound healing, thereby enhancing the medical aesthetic effect. At the same time, it can also inhibit neutrophil aggregation, inhibit hyperpigmentation and acne caused by postoperative inflammation. Description of the Drawings

[0028] Figure 1 It shows the improvement of facial flushing before and after medical aesthetics for Application Example 1 and Comparative Application Example 6. Detailed Embodiments

[0029] To better illustrate the purpose, technical solution and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0030] The sources of the raw materials used in the following examples and comparative examples are as follows:

[0031] Oat β-glucan: The manufacturer is Beijing Oriental Miaosen Biotechnology Co., Ltd., and the trade name is Miaokexiu;

[0032] Oat kernel extract: The manufacturer is Symrise Flavors & Fragrances (Nantong) Co., Ltd., and the trade name is Avena;

[0033] Holy basil leaf extract: The manufacturer is Shaanxi Snow Biotech Co., Ltd.;

[0034] Hops extract: The manufacturer is BASF SE, and the trade name is Senseryn TM ;

[0035] Brazil nut seed extract: The manufacturer is Clariant Chemicals (China) Limited, and the trade name is EthienceProtect.

[0036] For other materials, reagents, etc. used in the examples, unless otherwise specified, they can all be obtained commercially.

[0037] Example 1

[0038] A repair and efficiency-enhancing composition applicable to non-invasive medical aesthetics, comprising oat β-glucan, oat kernel extract, holy basil leaf extract, hops extract, and brazil nut seed extract in a weight ratio of 1.1:1.8:1.3:0.5:0.9, and the total mass fraction of the oat β-glucan, oat kernel extract, holy basil leaf extract, hops extract, and brazil nut seed extract is 100 parts.

[0039] Example 2

[0040] A repair and efficiency-enhancing composition applicable to non-invasive medical aesthetics, comprising oat β-glucan, oat kernel extract, holy basil leaf extract, hops extract, and brazil nut seed extract in a weight ratio of 1:2:2:0.8:0.8, and the total mass fraction of the oat β-glucan, oat kernel extract, holy basil leaf extract, hops extract, and brazil nut seed extract is 100 parts.

[0041] Example 3

[0042] A repair and efficiency-enhancing composition applicable to non-invasive medical aesthetics, comprising oat β-glucan, oat kernel extract, holy basil leaf extract, hops extract, and brazil nut seed extract in a weight ratio of 1.2:1.5:1:0.1:1, and the total mass fraction of the oat β-glucan, oat kernel extract, holy basil leaf extract, hops extract, and brazil nut seed extract is 100 parts.

[0043] Example 4

[0044] A repair and efficiency-enhancing composition applicable to non-invasive medical aesthetics, comprising oat β-glucan, oat kernel extract, holy basil leaf extract, hops extract, and brazil nut seed extract in a weight ratio of 0.5:1:3:0.1:0.5, and the total mass fraction of the oat β-glucan, oat kernel extract, holy basil leaf extract, hops extract, and brazil nut seed extract is 100 parts.

[0045] Example 5

[0046] A repair and efficiency-enhancing composition applicable to non-invasive medical aesthetics, comprising oat β-glucan, oat kernel extract, holy basil leaf extract, hops extract, and brazil nut seed extract in a weight ratio of 2:2:1:1.2:1.2, and the total mass fraction of the oat β-glucan, oat kernel extract, holy basil leaf extract, hops extract, and brazil nut seed extract is 100 parts.

[0047] Example 6

[0048] A repair and efficiency enhancement composition applicable to non-invasive medical aesthetics, comprising oat β-glucan, oat kernel extract, holy basil leaf extract, hops extract and brazil nut seed extract in a weight ratio of 0.11:0.1:5:1:2, and the total mass fraction of the oat β-glucan, oat kernel extract, holy basil leaf extract, hops extract and brazil nut seed extract is 100 parts.

[0049] Example 7

[0050] A repair and efficiency enhancement composition applicable to non-invasive medical aesthetics, comprising oat β-glucan, oat kernel extract, holy basil leaf extract, hops extract and brazil nut seed extract in a weight ratio of 3:2:1:0.1:0.2, and the total mass fraction of the oat β-glucan, oat kernel extract, holy basil leaf extract, hops extract and brazil nut seed extract is 100 parts.

[0051] Comparative Example 1

[0052] The difference between Comparative Example 1 and Example 1 is that: the composition does not add oat β-glucan, and the missing amount is supplemented with oat kernel extract, holy basil leaf extract, hops extract and brazil nut seed extract in a weight ratio of 1.8:1.3:0.5:0.9.

[0053] Comparative Example 2

[0054] The difference between Comparative Example 2 and Example 1 is that: the composition does not add oat kernel extract, and the missing amount is supplemented with oat β-glucan, holy basil leaf extract, hops extract and brazil nut seed extract in a weight ratio of 1.1:1.3:0.5:0.9.

[0055] Comparative Example 3

[0056] The difference between Comparative Example 3 and Example 1 is that: the composition does not add holy basil leaf extract, and the missing amount is supplemented with oat β-glucan, oat kernel extract, hops extract and brazil nut seed extract in a weight ratio of 1.1:1.8:0.5:0.9.

[0057] Comparative Example 4

[0058] The difference between Comparative Example 4 and Example 1 is that: the composition does not add hops extract, and the missing amount is supplemented with oat β-glucan, oat kernel extract, holy basil leaf extract and brazil nut seed extract in a weight ratio of 1.1:1.8:1.3:0.9.

[0059] Comparative Example 5

[0060] The difference between Comparative Example 5 and Example 1 is that: the composition does not add brazil nut seed extract, and the missing amount is supplemented with oat β-glucan, oat kernel extract, holy basil leaf extract and hops extract in a weight ratio of 1.1:1.8:1.3:0.5.

[0061] Test Example 1: Promotion of collagen regeneration and barrier function test

[0062] The compositions of Examples 1-7 and Comparative Examples 1-5 were set as 12 groups of test sample groups, and the experiments were carried out according to the following steps. The specific steps are as follows:

[0063] 1. Cell line and culture conditions

[0064] Cell line: Human immortalized keratinocytes (HaCaT, catalog number YN2020, Shanghai Yaji Biotechnology Co., Ltd.), human dermal fibroblasts (HSF, catalog number C1101P, Guangzhou Geneo Biotechnology Co., Ltd.). All cells were identified by STR and were free of mycoplasma contamination.

[0065] Culture conditions: A constant temperature incubator at 37 ± 0.5 °C, saturated humidity (95% ± 2%), and 5% CO2 environment.

[0066] 2. Sample treatment

[0067] Preparation of sample treatment solution: Dissolve the compositions of Examples 1-7 and Comparative Examples 1-5 in dimethyl sulfoxide (DMSO, final concentration ≤ 0.1%), and adjust to a final concentration of 0.1% (v / v) by gradient dilution method (DMEM high-glucose medium containing 10% fetal bovine serum), and filter sterilize with a 0.22 μm filter membrane for standby to obtain the sample treatment solutions of each group.

[0068] 3. Cell resuscitation and culture

[0069] Take HaCaT cells (Shanghai Yaji Bio) and HSF cells (Guangzhou Geneo Bio) in the logarithmic growth phase, and inoculate them into 96-well culture plates at a density of 2000 cells / well. Add 100 μL of DMEM complete medium (containing 10% FBS + 1% double antibody) to each well, and place it in a 37 °C incubator for static culture for 24 h to form a monolayer of adherent cells;

[0070] 4. Sample treatment

[0071] Sampling operation: Discard the supernatant of the resuscitated and cultured cells, then group them into a blank group and an experimental group: In the blank group, continue to inject 100 μL of DMEM complete medium (containing 10% FBS + 1% double antibody, v / v) into each well of the culture plate. In the experimental group, continue to inject 100 μL of DMEM complete medium containing 0.1% corresponding sample solution into each well of the culture plate. After continuous culture for 24 h, collect the cells of each group, aspirate and discard the medium, and wash the cells 2 times with PBS. Add 1 mL of TRIzol lysate to each well, scrape the cells and transfer them to an EP tube, and store them at -80 °C for later use.

[0072] 5. Protein expression detection (ELISA)

[0073] Claudin-1 detection: Operate according to the instructions of the human Claudin-1 ELISA kit (CSB-EL005490HU, Shanghai Huamei Biological Engineering Co., Ltd.) to determine the content of Claudin-1 in the HaCaT cell lysate.

[0074] Type I collagen detection: Operate according to the instructions of the human Col I ELISA kit (ml057630, Shanghai Enzyme-linked Biotechnology Co., Ltd.) to determine the content of Col I in the HSF cell lysate.

[0075] 6. Calculation of protein expression promotion rate

[0076]

[0077] The results of the final protein expression promotion rate are shown in Table 1.

[0078] Table 1 Promotion rates of type I collagen and tight junction proteins in samples of each group

[0079]

[0080]

[0081] This test directly verifies the promoting effect of the composition on type I collagen and tight junction proteins by detecting the promotion rates of type I collagen and tight junction proteins. This test evaluates the role of the composition in increasing skin tolerance before medical aesthetics and promoting skin repair after surgery by verifying the effect of the composition on tight junction proteins in epidermal keratinocytes and collagen in the dermis.

[0082] From the data of Example 1 and Comparative Examples 1-5 in Table 1, it can be seen that the composition of Example 1 with the five components of oat β-glucan, oat kernel extract, holy basil leaf extract, hop extract and brazil nut seed extract compounded has a significant improvement in the promotion rate of type I collagen and the promotion rate of tight junction protein compared with the compositions of Comparative Examples 1-5. This shows that the five components of oat β-glucan, oat kernel extract, holy basil leaf extract, hop extract and brazil nut seed extract have a synergistic effect. By up-regulating the tight junction protein Claudin-1, it can strengthen the barrier integrity; by promoting the synthesis of type I collagen (Collagen I), it can enhance the toughness of the dermis, so as to improve the barrier defense ability to resist damage before surgery, accelerate the barrier reconstruction after surgery, reduce the sensitivity to stimulation and promote the wound healing.

[0083] Combined with the data of Examples 1-7, it can be seen that when the weight ratio of oat β-glucan, oat kernel extract, holy basil leaf extract, hop extract and brazil nut seed extract is (1-1.2):(1.5-2):(1-2):(0.1-0.8):(0.8-1), the composition has a better effect in promoting the generation of type I collagen and tight junction protein.

[0084] Test Example 2: Inhibition test of Staphylococcus aureus and Propionibacterium acnes

[0085] 1. Preparation of experimental materials

[0086] Biological samples: Healthy zebrafish embryos 6-8 hours after fertilization (screening criteria: normal morphology and developed to blastula stage).

[0087] Reagents:

[0088] Zebrafish embryo culture medium (containing methylene blue bacteriostatic agent), mixed bacterial solution of Staphylococcus aureus and Propionibacterium acnes (final concentration 1×10 7 –5×10 7 CFU / mL, mixed at a volume ratio of 1:1), test samples of the compositions of Examples 1-7 and Comparative Examples 1-5 (mass concentration of 10%, dissolved in embryo culture medium)

[0089] Zebrafish embryo culture medium: Weigh 2940 mg of anhydrous calcium chloride, 1233 mg of magnesium sulfate heptahydrate, 630 mg of sodium bicarbonate, 55 mg of potassium chloride, and 1 g of methylene blue and dissolve them in 10 L of water. The pH value is 6.5-8.5, and all chemicals are of analytical pure grade.

[0090] 2. Experimental grouping

[0091] The washed and healthy zebrafish embryos 6-8 hours after fertilization were grouped and operated as follows:

[0092] Blank group: Zebrafish developed normally. During the culture process, 200 μL of zebrafish embryo culture medium was added to each well.

[0093] Control group: 10 μL of mixed bacterial solution was injected into the tail fin of zebrafish before culture. During the culture process, 200 μL of embryo culture medium was added to each well.

[0094] Sample group: 10 μL of mixed bacterial solution was injected into the tail fin of zebrafish before culture. During the culture process, 200 μL of embryo culture medium containing 10 wt% composition was added to each well.

[0095] 3. Culture conditions

[0096] The blank group, control group and sample group were respectively placed in 24-well plates for culture, with at least 10 embryos in each group. After treating healthy zebrafish embryos according to the above grouping conditions, they were placed in a constant temperature incubator and cultured at 28 °C for 3 h.

[0097] 4. Data collection and analysis

[0098] Fluorescence imaging: Randomly select 10 zebrafish from each group, place them under a fluorescence microscope to take fluorescence images of the dorsal region of zebrafish, analyze and collect data using the advanced image processing software Image J. Use the rectangular tool to accurately demarcate the target area, and use the gray intensity method to measure the average fluorescence intensity of the selected area, which is the number of neutrophils in zebrafish.

[0099] Neutrophil quantification: Analyze and collect data using the advanced image processing software Image J. Use the rectangular tool to accurately demarcate the target area, and use the gray intensity method to measure the average fluorescence intensity of the selected area, which is the number of neutrophils in zebrafish.

[0100] 5. Calculation of the inhibition rate of zebrafish neutrophils

[0101] Specific data are shown in Table 2.

[0102] Table 2 Inhibition rate data of zebrafish neutrophils in each group of samples

[0103]

[0104]

[0105] The above test established an inflammatory model of zebrafish induced by Staphylococcus aureus and Propionibacterium acnes. The test sample was administered in aqueous solution, causing an immune response in the neutrophils in the zebrafish, migrating and aggregating towards the skin epidermis. Then, by detecting the inhibition rate of zebrafish neutrophils, the aggregation of zebrafish skin neutrophils could be visually obtained, thereby determining whether the sample group could inhibit the neutrophil aggregation induced by Propionibacterium acnes in zebrafish. The higher the inhibition rate of zebrafish neutrophils, the more the composition could inhibit the inflammation induced by Staphylococcus aureus and Propionibacterium acnes, that is, it could prove its effect of inhibiting inflammation and preventing acne.

[0106] As can be seen from Table 2, combining the data of Example 1 and Comparative Examples 1-5, it can be known that the composition of Example 1 formulated with five components of oat β-glucan, oat kernel extract, holy basil leaf extract, hops extract and brazil nut seed extract has a significant improvement in the inhibition rate of zebrafish neutrophils compared with the compositions of Comparative Examples 1-5, indicating that the five components of oat β-glucan, oat kernel extract, holy basil leaf extract, hops extract and brazil nut seed extract can synergistically inhibit the aggregation of zebrafish neutrophils, thereby inhibiting the production of inflammatory factors, and can play the role of inhibiting inflammation and preventing acne.

[0107] Combining the data of Examples 1-7, it can be known that when the weight ratio of oat β-glucan, oat kernel extract, holy basil leaf extract, hops extract and brazil nut seed extract is (1-1.2):(1.5-2):(1-2):(0.1-0.8):(0.8-1), the composition has a better effect in inhibiting inflammation.

[0108] Application Examples 1-7 and Comparative Application Examples 1-5

[0109] The compositions of Examples 1-7 and Comparative Examples 1-5 were added to the essence at a concentration of 3 wt% respectively to obtain the essence of Application Examples 1-7 and Comparative Application Examples 1-5, and the formula is shown in Table 3.

[0110] The preparation method of the essence of Application Examples 1-7 and Comparative Application Examples 1-5 includes the following steps:

[0111] S1. Mix the humectant, thickener and 1 / 2 amount of deionized water, and homogenize at 80 °C to obtain a mixed solution;

[0112] S2. Wait for the temperature of the mixed solution in S1 to drop to 60 °C, add the preservative, and when the system temperature drops to 40 °C, add each component in the composition and the remaining deionized water, stir evenly, and finally add the pH regulator to adjust the pH to 6 to obtain the essence.

[0113] Table 3 Formulation of the essence of Application Examples 1-7 and Comparative Application Examples 1-5

[0114]

[0115] Comparative Application Example 6

[0116] The essence of comparative application example 6 does not add the repairing and synergistic composition suitable for non-invasive medical aesthetics, and an equal amount of deionized water is used to replace the composition. The preparation method is the same as that of application example 1.

[0117] Test Example 3: Efficacy test of serum

[0118] The experiment content is: According to the "Cosmetic Safety Technical Specifications" (2015), subjects aged 20-50 were selected to conduct post-photorejuvenation repair and soothing experiments. 13 groups were randomly selected, with 4 people in each group. All subjects clearly stated the experimental requirements and agreed to undergo photorejuvenation on their faces and complete this test. When choosing the photorejuvenation M22 device, the hospital operating technician will adjust the intensity of use according to the specific facial conditions and different areas of the subjects.

[0119] This test is divided into two stages. The first stage is before photorejuvenation:

[0120] The subjects measured the basic values of facial physiological data 20 days before the operation. After that, the subjects used samples (the samples were the essences of application examples 1-7 and comparative application examples 1-6) on the whole face once in the morning and evening every day until the day of photorejuvenation, and then visited to collect facial physiological index data. Before the operation, the subjects needed to collect the transepidermal water loss value TEWL and the percentage of facial red zone pixels before and after use;

[0121] The second stage is after photorejuvenation: After photorejuvenation, the subjects measured the physiological index data of their faces, and then used the samples once a day in the morning and evening on the whole face. After 20 days, they visited again to collect the facial physiological index data. The facial physiological data collected before the operation were: transepidermal water loss value TEWL and facial red area pixel ratio;

[0122] The method of collecting facial physiological index data is as follows:

[0123] Before and after the photorejuvenation, the subjects sat in an air-conditioned room with a temperature of 21±1℃ and a humidity of 50±10% for 30 minutes. CM 825 tests the transepidermal water loss value TEWL of the zygomatic bone. Photorejuvenation is a non-invasive medical aesthetic project, so redness cannot be observed with the naked eye after the operation. Therefore, the VISA-CR instrument is used to photograph the red area of the subject's face, analyze the proportion of facial red area pixels in the total effective facial area pixels, and quantify the deep redness of the skin. After the operation, the skin color test probe Colorimeter CL400 is used to quantify the glossiness L* value of the subject's facial zygomatic bone.

[0124] The calculation formula is as follows:

[0125] Preoperative improvement rate of TEWL value (%) = |(Preoperative TEWL value 使用后 - Preoperative TEWL value 使用前 )| / Preoperative TEWL value 使用前 × 100%;

[0126] Postoperative improvement rate of TEWL value (%) = |(Postoperative TEWL value 使用后 - Postoperative TEWL value 使用前 )| / Postoperative TEWL value 使用前 × 100%;

[0127] Preoperative improvement rate of red area pixel ratio (%) = |(Preoperative red area pixel ratio improvement rate 使用后 - Preoperative red area pixel ratio improvement rate 使用前 )| / Preoperative red area pixel ratio improvement rate 使用前 × 100%;

[0128] Postoperative improvement rate of red area pixel ratio (%) = |(Postoperative red area pixel ratio improvement rate 使用后 - Postoperative red area pixel ratio improvement rate 使用前 )| / Postoperative red area pixel ratio improvement rate 使用前 × 100%;

[0129] Postoperative improvement of zygomatic bone gloss L* value (%) = |(Postoperative zygomatic bone gloss L* value 使用后 - Postoperative zygomatic bone gloss L* value 使用前 )| / Postoperative zygomatic bone gloss L* value 使用前 × 100%; The calculation results are shown in Table 4.

[0130] Table 4 Human experiment results of each essence

[0131]

[0132] The ability of the composition to strengthen the barrier before aesthetic medicine is represented by the preoperative improvement rate of TEWL value and the preoperative improvement rate of red area pixel ratio. The repair ability after aesthetic medicine is represented by the postoperative improvement of TEWL value and the postoperative improvement rate of red area pixel ratio. The postoperative synergistic effect and prevention of reverse darkening are represented by the postoperative improvement of zygomatic bone gloss L* value. The better the above improvement rate, the better the corresponding ability.

[0133] It can be seen from Figure 1 that the facial flushing situation of the essence in Application Example 1 after 20 days of preoperative use and 20 days of postoperative use has been significantly improved compared with the essence in Comparative Application Example 6, which shows that the composition of the present invention can achieve the effects of barrier repair, redness reduction, and prevention of reverse darkening before and after aesthetic medicine.

[0134] As can be seen from the data of Application Example 1 and Comparative Application Examples 1-5 in Table 4, the essence of Application Example 1, which selects a composition containing oat β-glucan, oat kernel extract, holy basil leaf extract, hops extract and brazil nut seed extract, has significantly improved effects in barrier repair, redness reduction, prevention of reverse darkening and postoperative synergistic enhancement compared with Comparative Application Examples 1-5 that lack one of the above components. This shows that the above five components can synergistically strengthen the barrier, inhibit inflammation, prevent pigmentation, reduce the risk of acne after surgery and promote skin regeneration. This may be because oat β-glucan can form a protective film on the skin surface and promote the expression of related barrier factors to jointly reduce the impact of external stimuli on the skin; the oat kernel extract blocks the NF-κB signaling pathway and inhibits the release of pro-inflammatory factors such as IL-6 and TNF-α; the holy basil leaf extract blocks the melanin synthesis pathway by inhibiting the activity of tyrosinase and reducing dopaquinone intermediates, and at the same time scavenges ROS to protect dermal fibroblasts and reduce the incidence of postoperative pigmentation; the brazil nut seed extract activates the PPAR-γ pathway to promote the secretion of lamellar bodies, accelerate epidermal lipid metabolism, avoid compensatory pigmentation caused by barrier defects, and at the same time, the selenoprotein in the brazil nut seed extract promotes the differentiation and maturation of keratinocytes by enhancing the stability of desmoglein and accelerates the repair of damaged cells; the hops extract remodels the balance of the skin surface flora, reduces the proliferation of pathogenic bacteria such as Staphylococcus aureus, prevents acne caused by skin flora disorders, and reduces neurogenic inflammatory responses by regulating transient receptor potential (TRPV1) and reduces postoperative erythema. In summary, the composition of the present invention achieves a full-cycle closed-loop management of "preoperative defense - postoperative repair - effect maintenance" for non-invasive medical aesthetics through a multi-dimensional synergistic mechanism, strengthening the skin barrier before surgery to increase tolerance, repairing and enhancing the effect after surgery to prevent pigmentation and acne.

[0135] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit the protection scope of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A repair and efficiency-enhancing composition applicable to non-invasive medical aesthetics, characterized in that It comprises the following components: oat β-glucan, oat kernel extract, holy basil leaf extract, hops extract and brazil nut seed extract, and the weight ratio of the oat β-glucan, oat kernel extract, holy basil leaf extract, hops extract and brazil nut seed extract is (0.11-3):(0.1-2):(1-5):(0.1-1):(0.2-2).

2. The repair and efficiency enhancing composition applicable to non-invasive medical aesthetics as described in claim 1, wherein The weight ratio of the oat β-glucan, oat kernel extract, holy basil leaf extract, hops extract and brazil nut seed extract is (0.5-2):(1-2):(1-3):(0.1-1.2):(0.5-1.2).

3. The repair and enhancement composition applicable to non-invasive medical aesthetics according to claim 1, characterized in that, The weight ratio of the oat β-glucan, oat kernel extract, holy basil leaf extract, hops extract and brazil nut seed extract is (1-1.2):(1.5-2):(1-2):(0.1-0.8):(0.8-1).

4. Use of the repair and synergistic composition applicable to non-invasive medical aesthetics according to any one of claims 1-3 in the preparation of cosmetics.

5. Use of the repair and enhancement composition applicable to non-invasive medical aesthetics according to claim 4 in the preparation of cosmetics, characterized in that, The cosmetics are lotion, emulsion, cream, facial mask, essence or spray, and the addition amount of the composition is 1%-5% of the total weight of the cosmetics.

6. An essence, characterized in that, It comprises raw materials in the following weight percentages: 1%-5% of the repair and synergistic composition applicable to non-invasive medical aesthetics according to any one of claims 1-3, 0.05%-0.5% of thickener, 3%-10% of humectant, 0.5%-3% of preservative and 0.01%-0.3% of pH regulator, and the balance is deionized water.

7. The essence according to claim 6, characterized in that, The raw materials are selected from at least one of (a)-(d): (a) The thickener includes at least one of xanthan gum, carbomer, acryloyldimethyltaurate / VP copolymer, ammonium acryloyldimethyltaurate / VP copolymer and sclerotium gum; (b) The humectant includes at least one of allantoin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, D-panthenol, sodium hyaluronate, 1,2-butanediol, glycerol, pullulan and ceramide; (c) The pH regulator includes at least one of arginine, tromethamine and disodium EDTA; (d) The preservative includes at least one of 1,3-propanediol, 1,2-hexanediol and p-hydroxyacetophenone.

8. The preparation method of the essence according to claim 7, characterized in that, It comprises the following steps: S1. Mix the humectant, thickener and part of the deionized water, homogenize at 75-85°C, then add the preservative and stir evenly to obtain a mixture; S2. Wait until the temperature of the mixture in S1 drops to 35-45°C, add the components in the repair and synergistic composition applicable to non-invasive medical aesthetics and the remaining deionized water, stir evenly, and finally add the pH regulator to adjust the pH to obtain the essence.

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