Compound skin care micro formula based on exocarpium citri grandis and application thereof

Abstract

The invention provides a compound skin care micro-formula based on exocarpium citri grandis and application thereof, and belongs to the field of skin care products. The composition comprises a pomelo fruit extract, a notopterygium root extract, an African chandelia fruit extract, a crambe abyssinica leaf extract and a delivery substance. According to the invention, four bioactive pathways are integrated to form a cascade repair network; and a traditional plant compounding framework is broken through, and two types of novel functional components are introduced. Meanwhile, aiming at the transdermal problem of fat-soluble components such as naringin, a micro-emulsion delivery system is developed, and the particle size is controlled: the micro-emulsion system is not layered for 6 months in a 40 DEG C / RH 75% acceleration test. The composition has both independent use and compounding compatibility, and can meet diversified market requirements.

Description

Technical Field

[0001] The present invention belongs to the field of skin care products, and particularly relates to a compound skin care micro-formulation based on tangerine peel and its application. Background Art

[0002] In recent years, the sensitive skin care market has shown a rapid growth trend, and consumers increasingly favor skin care ingredients with both natural mild characteristics and high anti-inflammatory activity. The current mainstream products on the market mostly focus on a single anti-inflammatory or barrier repair ingredient, suffering from the defects of single action target and insufficient multi-pathway synergistic mechanism.

[0003] The current patents on soothing and repairing raw materials related to cosmetics have the following significant defects: 1) The ingredient source is not natural. For example, CN115089521A, "A Soothing and Anti-inflammatory Composition, Soothing and Anti-inflammatory Cosmetics and Their Preparation Methods", uses chemically modified ingredients such as sodium carboxymethyl β-glucan, with a risk of synthetic residues; 2) The action pathway is single. For example, CN114469772A, "An Anti-inflammatory and Soothing Composition Based on Targeted Redness Repair and Its Preparation Method", relies on a single ingredient of asiaticoside, making it difficult to cover multiple mechanisms such as inhibition of inflammatory factors (TNFα / IL6), regulation of oxidative stress (ROS scavenging), and repair of barrier function (ceramide synthesis) simultaneously; 3) The synergistic effect is insufficient, and the prior art lacks in-depth exploration of the combined effects of natural ingredients.

[0004] Therefore, a natural and multi-pathway soothing and repairing composition is very necessary, and consumers in the market are more inclined to use such products.

[0005] The micro-formulation can better apply it to various products with different dosage forms and functions, having good compatibility and stability, excellent functionality, and also meeting the conditions for independent use. Summary of the Invention

[0006] The object of the present invention is to address the above industry pain points. This patent first proposes a "pomelo fruit - notopterygium root - kigelia africana fruit - crambe abyssinica leaf" four-dimensional synergistic system. This system innovatively combines: 1) Pomelo fruit flavonoids (inhibiting the TNFα / IL6 pathway); 2) Notopterygium ketones (analgesic and anti-allergic); 3) Kigelia africana fruit polyphenols (targeting ROS scavenging); 4) Crambe abyssinica leaf sphingolipids (promoting ceramide synthesis), forming a full-link action mechanism covering "inflammation inhibition - oxidative defense - barrier repair". Moreover, the micro-formulation of this patent has wide applicability, can be used alone as a product, or can be added to the formula of other functional products for synergistic effect.

[0007] To achieve the above object, the present invention provides a skin care micro-formulation, which includes the following components in parts by mass:

[0008]

[0009] Preferably, the delivery system comprises at least one of the following components:

[0010] Lecithin, soy lecithin, hydrogenated lecithin.

[0011] Preferably, the skin care micro-formulation is prepared by the following steps:

[0012] S1: Melting and mixing the pomelo fruit extract, notopterygium root extract, kigelia africana fruit extract, and crambe abyssinica leaf extract with the delivery system at 45 - 50 °C, stirring under a nitrogen atmosphere until a homogeneous and transparent oil phase is formed;

[0013] S2: Adding polyol to water, controlling the temperature at 25 ± 2 °C, and stirring until completely dissolved to obtain an aqueous solution;

[0014] S3: Injecting the oil phase into the aqueous phase and shearing for emulsification to obtain an emulsion;

[0015] S4: Introducing the primary emulsion into a high-pressure microfluidization device to obtain a microemulsion.

[0016] Preferably, the skin care micro-formulation further comprises polyol; the addition amount of the polyol is at least 30 parts; the polyol comprises at least one of the following components:

[0017] Glycerol, propylene glycol, butylene glycol.

[0018] Preferably, the pomelo fruit extract is extracted by supercritical CO2;

[0019] The specific process includes:

[0020] S1 Pretreatment: Hot air drying and pulverizing the pomelo fruit peel, and sieving;

[0021] S2 Supercritical CO2 extraction: The entrainer is ethanol water:

[0022] Extraction kettle: Temperature 45 °C, pressure 35 MPa, CO2 flow rate 25 kg / h;

[0023] Separation kettle I: Temperature 55 °C, pressure 8 Mpa;

[0024] Separation kettle II: Temperature 32 °C, pressure 5 Mpa;

[0025] S3 Post-treatment: Combining the products of separation kettle II, rotary evaporating to recover ethanol, and then freeze-drying.

[0026] Preferably, the kigelia africana fruit extract is the extract after enzymatic hydrolysis.

[0027] Preferably, the crambe abyssinica leaf extract is the subcritical water extract.

[0028] The present invention also provides the use of the aforementioned skin care micro-formula in the preparation of a skin external preparation; the skin care micro-formula has the effect of soothing and repairing the skin.

[0029] The pomelo fruit extract in the present invention is Huajuhong (also known as Huazhou pomelo, which comes from Huazhou City, Guangdong Province. It is a local specialty plant and has been certified as a Chinese national geographical indication product with extremely high edible and pharmacological value); it is called Huajuhong in the Pharmacopoeia of the People's Republic of China. It is said to have been planted in the Han Dynasty at the latest. It was prescribed by doctors at the latest in the Ming Dynasty. It was listed as a court tribute and imperial medicine in the Ming and Qing Dynasties. It is one of the "Top Ten Guangdong Medicines" and China's precious Chinese medicinal materials, and is known as "Southern Ginseng". Huajuhong is a tree of the genus Citrus in the Rutaceae family. Its immature dried fruit or the dried skin of the outer layer of the fruit has the effects of widening the middle and lower qi, dispersing cold and drying dampness, strengthening the stomach and promoting qi, eliminating phlegm and relieving cough, and relieving smoking and sobering up. The phlegm-removing and cough-relieving effect is particularly obvious. Huajuhong has such a miraculous effect, one is the active ingredients of the medicine it contains, and the other is that the soil in some areas of Huazhou is rich in real estate. The old prefecture records say: "There is real estate in Baoshan and the government offices in Huazhou City." "Real estate can dissolve phlegm, and tangerine peel has the energy of real estate, so it is more effective in dissolving diseases."

[0030] Modern pharmacological studies have confirmed that its pomelo fruit extract can inhibit the expression of pro-inflammatory factors such as TNF-α, IL-6, IL-1β, downregulate the phosphorylation level of MAPK and NF-κB signaling pathways, and promote the secretion of anti-inflammatory factors such as IL-10, forming a multi-target anti-inflammatory effect. It is worth noting that naringenin, as a representative component of polymethoxyflavonoids, has been proven to have unique anti-inflammatory mechanisms such as inhibiting mast cell degranulation and blocking the binding of LPS to immune cells. However, existing patented technologies are mostly limited to the development of the cough and phlegm-relieving effects of pomelo (such as CN03126908.7), and there has been no multi-dimensional efficacy system design based on modern skin pharmacology in the field of cosmetics.

[0031] The present invention integrates four major biologically active pathways to form a cascade repair network:

[0032] Pomelo fruit extract: By inhibiting NFκB nuclear translocation and activating the Nrf2 pathway, it simultaneously blocks the release of inflammatory factors and oxidative stress damage;

[0033] Notopterygium wilfordii extract: specifically inhibits COX-2 enzyme activity, speeding up the disappearance of erythema;

[0034] African chandelier tree fruit extract: Through high content of polyphenols, it can achieve ROS scavenging and Staphylococcus aureus inhibition;

[0035] Sea kale leaf extract: Promotes the expression of Claudin-1 protein and reduces TEWL value, accelerating the reconstruction of the physical barrier.

[0036] This invention breaks through the traditional plant compound framework and introduces two new types of effective ingredients:

[0037] African chandelier tree fruit: Ellagic acid was enriched by microwave-assisted extraction technology, which combined with metalloproteinase MMP-1 to significantly inhibit UV-induced collagen degradation;

[0038] Sea kale leaves: The active glucosinolate product is released by targeted enzymatic hydrolysis, and its efficacy in promoting the expression of keratin KRT1 is significantly higher than that of traditional Centella asiatica extract.

[0039] The present invention also develops an environmentally friendly active ingredient co-production technology:

[0040] Synchronous extraction process: The combined enrichment of pomelo flavonoids and chandelier tree fruit polyphenols was achieved through ethanol gradient extraction (50% → 70% → 90%), and the solvent consumption was reduced by 35%;

[0041] Membrane separation and purification: Using 100kDa ceramic membrane coupled with nanofiltration technology, the COD value of waste liquid is reduced from 8500mg / L to 120mg / L, meeting industrial emission standards;

[0042] Low-temperature freeze-drying process: -45℃ quick freezing is used in the pre-freezing stage, so that the degradation rate of heat-sensitive ingredients (such as notopterygium wilfordii alcohol) is less than 2%, which saves 41% energy compared with traditional spray drying.

[0043] The present invention has the following advantages:

[0044] It integrates four major bioactive pathways to form a cascade repair network; and breaks through the traditional plant compound framework and introduces two new types of effective ingredients.

[0045] At the same time, the present invention aims at the problem of transdermal delivery of fat-soluble ingredients such as naringin and develops a microemulsion delivery system: Particle size control:

[0046] A uniform particle size of about 82.6 nm was obtained through a high-pressure homogenization process (1000 bar×3 cycles), and the cumulative permeation amount was higher than that of ordinary emulsion.

[0047] Enhanced stability: The microemulsion system remained free of stratification for 6 months in an accelerated test at 40°C / RH75%, and the active ingredients were largely retained.

[0048] The present invention has both independent use and compound compatibility, and can meet diverse market demands:

[0049] The present invention can be used alone as a highly effective repair essence and directly applied to sensitive or damaged skin, achieving the triple effects of "anti-inflammatory - anti-oxidation - barrier repair" through a quaternary active system.

[0050] The active ingredient concentration (8.5-18wt%) can be adjusted to meet different efficacy intensity requirements (such as daily care or post-operative repair).

[0051] The microemulsion system of the present invention has good compatibility with common cosmetic matrices (such as gels, creams, sprays), and an addition amount of 5-15% can enhance the repair function of the formulation. For example, when this microemulsion is added to a sunscreen at a ratio of 10%, the UV damage repair ability can be improved synchronously.

[0052] The pH value (6.5-7.0) of the micro-formulation of the present invention matches that of mainstream cosmetic formulations, and there is no need to adjust the production process additionally. Description of the Drawings

[0053] Figure 1 It is a diagram of the experimental results of the repair effect on zebrafish.

[0054] Figure 2 It is a bar chart of the fluorescence intensity of the yolk sac for the antioxidant effect test on zebrafish. Detailed Description of the Invention

[0055] The technical solutions in the embodiments of the present invention will be described clearly and completely below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative efforts shall fall within the protection scope of the present invention.

[0056] In the description of the present invention, the terms "first" and "second" are only used for descriptive purposes, and cannot be construed as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include one or more of the described features. In the description of the present invention, "a plurality" means two or more, unless otherwise specifically defined.

[0057] In the description of the present invention, the term "for example" is used to mean "serving as an example, illustration, or explanation". Any embodiment described as "for example" in the present invention is not necessarily construed as being more preferred or having more advantages than other embodiments. In order to enable any person skilled in the art to implement and use the present invention, the following description is given. In the following description, details are set forth for purposes of explanation. It should be understood that those skilled in the art can recognize that the present invention can be implemented without using these specific details. In other instances, well-known structures and processes will not be described in detail to avoid obscuring the description of the present invention with unnecessary details. Therefore, the present invention is not intended to be limited to the embodiments shown, but is consistent with the broadest scope that conforms to the principles and features disclosed in the present invention.

[0058] Unless otherwise specified, the experimental methods used in the detailed description of the invention are all conventional methods, and the materials, reagents, etc. used, unless otherwise specified, can all be obtained from commercial channels.

[0059] In the present invention, unless otherwise specified, "%" represents mass percentage; the raw materials, reagents, etc. used are all conventional commercially available products.

[0060] In the present invention, the water used is preferably pure water (RO water).

[0061] In the present invention, the pomelo fruit extract is the extract of Exocarpium Citri Grandis; the INCI name is Extract of Citrus Grandis.

[0062] In the present invention, the extract of Notopterygium incisum has the INCI name of Extract of Notopterygium incisum.

[0063] In the present invention, the extract of Kigelia africana fruit has the INCI name of Extract of Kigelia africana Fruit.

[0064] In the present invention, the extract of Crambe maritima leaves has the INCI name of Extract of Crambe maritima Leaves.

[0065] In the present invention, the extract of Citrus paradisi has the INCI name of Extract of Citrus paradisi.

[0066] In the present invention, the extract of Ligusticum chuanxiong has the INCI name of Extract of Ligusticum chuanxiong.

[0067] In the present invention, the extract of Adansonia digitata fruit has the INCI name of Extract of Adansonia digitata Fruit.

[0068] In the present invention, the extract of Brassica oleracea acephala leaves has the INCI name of Extract of Brassica oleracea acephala Leaves.

[0069] The preparation method of the pomelo fruit extract in the present invention is as follows:

[0070] Raw material pretreatment: The pomelo fruit peel (from Rutaceae, produced in Huazhou, Guangdong) is dried at 60 °C by hot air until the water content ≤ 8%, and then pulverized and sieved through a 40-mesh sieve.

[0071] Process parameters:

[0072] Extraction kettle: temperature 45 °C, pressure 35 MPa, CO2 flow rate 25 kg / h

[0073] Separation kettle I: temperature 55 °C, pressure 8 MPa (collecting waxy components)

[0074] Separation kettle II: temperature 32 °C, pressure 5 MPa (collecting the target flavonoid components)

[0075] Entrainer: ethanol-water (8:2, v / v), dosage is 15% of the raw material amount

[0076] Post-treatment: Combine the products of Separation Kettle II, recover ethanol by rotary evaporation at 40°C, and obtain off-white powder by freeze-drying.

[0077] The preparation method of the Notopterygium incisum extract in the present invention is as follows:

[0078] Extraction system: The raw material (rhizome of Notopterygium incisum of Umbelliferae) and 70% ethanol are fed at a ratio of 1:12 (w / v)

[0079] Ultrasonic parameters: frequency 40 kHz, power 800 W, temperature 50°C, time 45 min

[0080] Purification process:

[0081] Macroporous resin AB-8 column chromatography (diameter-height ratio 1:8)

[0082] Elution procedure: 3 BV of water → 5 BV of 30% ethanol (for impurity removal) → 6 BV of 75% ethanol (collect the target component)

[0083] Concentration and drying: Concentrate under reduced pressure until the alcohol smell disappears, and obtain light yellow powder by spray drying (inlet air 180°C / outlet air 85°C).

[0084] The preparation method of the extract of Kigelia africana fruit in the present invention is as follows:

[0085] Enzymolysis system:

[0086] Raw material pretreatment: The fruit slices are quickly frozen in liquid nitrogen and then ultra-finely pulverized (particle size ≤ 50 μm)

[0087] Compound enzyme ratio: cellulase (20 U / mg): pectinase (15 U / mg): β-glucosidase (10 U / mg) = 3:2:1.

[0088] Enzymolysis conditions: pH 4.8, temperature 45°C, time 6 h, solid-liquid ratio 1:15.

[0089] Microwave-assisted extraction: power 600 W, pulse mode (30 s on / 15 s off), total time 8 min

[0090] Separation and purification: Centrifugation (8000 rpm, 15 min) → The supernatant is dynamically adsorbed by XDA-1 resin → Elution with 60% acetone

[0091] The preparation method of the extract of Crambe abyssinica leaves in the present invention is as follows:

[0092] Water subcritical extraction is adopted, and the subcritical parameters are:

[0093] The temperature of the reactor is 120°C and the pressure is 0.8 Mpa.

[0094] The water-to-material ratio is 20:1 (v / w), and dynamic cyclic extraction is performed 3 times (15 minutes each time).

[0095] Lipid separation:

[0096] Centrifugal separation (10,000 rpm, 20 minutes), and the upper lipid phase is taken.

[0097] Silica gel column chromatography (eluent: chloroform - methanol - water = 65:25:4);

[0098] Low-temperature crystallization (-20°C, standing for 12 hours) to obtain a milky white solid.

[0099] For the preparation method of the microemulsion, weigh each raw material according to the parts by mass in Table 1 below;

[0100] ① Preparation of the oil phase (operated under inert gas protection)

[0101] Melt and mix the active ingredient system and the delivery system (hydrogenated lecithin) at 45 - 50°C, maintain a nitrogen atmosphere (oxygen content ≤ 50 ppm), and the stirring rate is 200 rpm until a homogeneous and transparent oil phase is formed (turbidity ≤ 10 NTU).

[0102] ② Preparation of the water phase (low-temperature dispersion process)

[0103] Add the moisturizing system (polyol) and the natural preservative (1,2 - hexanediol) to deionized water with a conductivity ≤ 5 μS / cm, control the temperature at 25 ± 2°C, and stir at 500 rpm until completely dissolved to obtain an aqueous solution (viscosity 10 - 15 cP).

[0104] ③ Primary emulsification (high-shear dynamic emulsification)

[0105] Inject the oil phase into the water phase at a constant rate of 1 mL / s, and simultaneously start the high-speed shear emulsifier: to obtain an emulsion;

[0106] First stage: Pre-emulsify at 4000 rpm for 2 minutes (D90 particle size ≈ 500 nm)

[0107] Second stage: Main emulsify at 8000 rpm for 5 minutes (D50 particle size drops to 200 ± 20 nm, PDI ≤ 0.35)

[0108] Cooling cycle: Maintain the system temperature ≤ 30°C (jacket water cooling system ΔT = 5°C)

[0109] ④ Nanometerization treatment (precision control of high-pressure microfluidics)

[0110] Introduce the primary emulsion into the high-pressure microfluidics equipment, control the parameters: to obtain a microemulsion.

[0111] Homogeneous pressure: 150 MPa ± 5 Mpa

[0112] Number of cycles: 3 times (cooled to 25 °C at intervals each time)

[0113] Discharge index: D50 = 80 ± 5 nm

[0114] Table 1 Preparation ratio of microemulsion

[0115]

[0116]

[0117] Table 1 (continued) Preparation ratio of microemulsion

[0118] Component Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 Comparative Example 8 Exocarpium Citri Grandis Extract 1.575 1.4 0 1.225 1.225 1.225 Notopterygium Root Extract 1.05 1.05 0.875 0 0.875 0.875 Kigelia Fruit Extract 0 0.875 0.7 0.7 0 0.7 Crambe Leaf Extract 0.7 0 0.525 0.525 0.525 0 Grapefruit Fruit Extract 0 0 1.225 0 0 0 Chuanxiong Rhizome Extract 0 0 0 0.875 0 0 Baobab Fruit Extract 0 0 0 0 0.7 0 Kale Leaf Extract 0 0 0 0 0 0.525 Hydrogenated Lecithin 1 1 1 1 1 1 Polyol 3 3 3 3 3 3 Hexylene Glycol 0.5 0.5 0.5 0.5 0.5 0.5

[0119] The above-prepared composition was experimentally tested as follows:

[0120] Zebrafish repair efficacy test:

[0121] System and sample size:

[0122] Test system: Wild-type AB strain zebrafish

[0123] Zebrafish age: 3 days post-fertilization (3 dpf)

[0124] Sample size per group of experiments: 20 tails (N = 10)

[0125] Adult fish rearing and breeding method: According to the laboratory standard rearing and breeding method of our company

[0126] Principle method:

[0127] Zebrafish caudal fin regeneration is divided into three processes: wound healing, blastema formation, and regeneration outcome, with blastema formation being the most core. The cells for fin regeneration have multiple sources, and various cell types including epidermal cells, fibroblasts, and osteoblasts all promote caudal fin regeneration, and these cells have a high degree of lineage restriction. The regeneration of zebrafish caudal fin is similar to the repair mechanisms of human skin, bone, blood vessels, etc. Due to the characteristics of simple structure, easy surgical operation, no impact on survival after surgery, and convenient observation, the zebrafish caudal fin has become an important model for studying the tissue regeneration process. Therefore, by cutting the caudal fin with a scalpel perpendicular to the trunk, the quantitative result of the regenerated caudal fin area of zebrafish can evaluate whether the sample has repair efficacy

[0128] Experimental steps:

[0129] 1. A zebrafish tissue regeneration model was established by using a scalpel to cut the caudal fin. Zebrafish with the same degree of caudal fin injury were randomly grouped in a 6-well plate, with 20 tails in each well.

[0130] 2. The sample was administered in aqueous solution. At the same time, a normal control group and a model control group were set up, and the volume of each well was 3 mL.

[0131] 3. Incubate in the dark at 28.5 °C for 48 h.

[0132] 4. Randomly select 10 zebrafish from each experimental group and place them under a stereomicroscope for photographing. Use advanced image processing software to analyze and collect data. Analyze the caudal fin area (A) of zebrafish, calculate the repair efficacy of the sample according to the formula, and judge whether it has a repair efficacy.

[0133]

[0134] Judgment rule: In statistical analysis, p < 0.05 is judged to have a significant difference.

[0135] The results of Example 1 are as Figure 1 shown in Table 2. After testing, the caudal fin area of zebrafish in the sample group with a dosage of 0.1% was significantly increased compared with the model control group, and the caudal fin regeneration area was significantly increased compared with the model group (p < 0.05), revealing that the sample has a repair efficacy.

[0136] Table 2 Zebrafish repair efficacy test (dosage 0.1%)

[0137]

[0138] Zebrafish antioxidant efficacy test:

[0139] System and sample size:

[0140] Test system: Melanin allele mutant translucent Albino strain zebrafish.

[0141] Zebrafish age: 2 days post-fertilization (2 dpf).

[0142] Sample size for each group of experiments: 15 tails (N = 10)

[0143] Adult fish rearing and breeding method: According to the laboratory standard rearing and breeding method of our company.

[0144] Principle method:

[0145] Reactive oxygen species (ROS) are oxygen-containing chemically reactive substances, including peroxides, superoxides, hydroxyl radicals, etc. A sharp increase in ROS levels may cause serious damage to cell structure, which is called oxidative stress. Menadione can generate reactive oxygen free radicals, and when the amount of free radicals generated is greater than the body's scavenging ability, an oxidative stress response will occur. In this experiment, the antioxidant efficacy of the sample was evaluated by the fluorescence intensity of zebrafish yolk sac.

[0146] Experimental procedure:

[0147] Randomly select zebrafish and place them in a 6-well plate, with 15 tails in each well.

[0148] Add menadione to establish a zebrafish oxidative stress model.

[0149] Administer the sample by dissolving it in water. At the same time, set up a normal control group and a model control group, with a volume of 3 mL in each well.

[0150] Incubate in the dark at 28.5 °C for 20 h.

[0151] Stain the zebrafish with a specific fluorescent reagent staining solution.

[0152] Randomly select 10 zebrafish from each experimental group and place them under a fluorescence microscope for photographing. Use advanced image processing software to analyze and collect data. Analyze the fluorescence intensity (S) of the zebrafish yolk sac, calculate the antioxidant efficacy value of the sample according to the formula, and judge whether it has antioxidant efficacy.

[0153]

[0154] Judgment rule: In statistical analysis, p < 0.05 is judged to be a significant difference.

[0155] The results of Example 1 are as Figure 2 shown in Table 3 and Table 4. Zebrafish antioxidant efficacy evaluation: The fluorescence intensity of the yolk sac of zebrafish in the sample group with a concentration of 0.0625% was significantly reduced compared with the model control group, revealing that the sample has antioxidant efficacy.

[0156] Table 3 Zebrafish antioxidant test (Example 1)

[0157]

[0158] Expand the tests for other examples and comparative examples, and the test methods are the same; the results are shown in Table 4 below.

[0159] Table 4 Zebrafish antioxidant test (expanded test, dosage 0.0625%)

[0160]

[0161] Comparative Examples 1, 2, 3, and 4 are default tests compared with Example 1; Comparative Examples 5, 6, 7, and 8 are component replacement tests compared with Example 2; According to the above experimental results, it can be seen that on the premise of the same content of active ingredients, the lack or replacement of any component in the present invention will cause a certain decline in its repair or antioxidant ability; This shows that the components in the composition of the present invention play a good synergistic effect, promoting and coordinating with each other and none of them can be missing. In addition, compared with some compositions with improper ratios, the composition provided by the present invention also has better skin brightening and antioxidant effects, indicating that the ratio of the composition has a significant impact on the effect, and the ratio of the composition provided by the present invention is a verified and preferred ratio.

[0162] The above detailed description is a specific description of one of the feasible embodiments of the present invention, and this embodiment is not intended to limit the patent scope of the present invention. Any equivalent implementation or change that does not depart from the present invention should be included within the scope of the technical solution of the present invention.

Claims

1. A skin care micro-formulation, characterized in that, Comprising the following components by mass parts:

2. The skin care micro-formulation according to claim 1, wherein The delivery system comprises at least one of the following components: Lecithin, soy lecithin, hydrogenated lecithin.

3. The skin care micro-formulation according to claim 1, wherein The skin care micro-formulation is made by the following steps: S1: Melting and mixing the pomelo fruit extract, Notopterygium incisum extract, Kigelia africana fruit extract, and Crambe abyssinica leaf extract with the delivery system at 45 - 50 °C, stirring under a nitrogen atmosphere; until a homogeneous and transparent oil phase is formed; S2: Adding polyol to water, controlling the temperature at 25 ± 2 °C, and stirring until completely dissolved to obtain an aqueous solution; S3: Injecting the oil phase into the aqueous phase and shearing for emulsification; obtaining an emulsion; S4: Introducing the primary emulsion into a high-pressure microfluidization device; obtaining a microemulsion.

4. The skin care micro-formulation according to claim 1, characterized in that, It also includes polyol; the addition amount of the polyol is at least 30 parts.

5. The skin care micro-formulation according to claim 3, characterized in that, The polyol comprises at least one of the following components: Glycerol, propylene glycol, butylene glycol.

6. The skin care micro-formulation according to claim 1, wherein The pomelo fruit extract is extracted by supercritical CO2; The specific process includes: S1 Pretreatment: Hot air drying and pulverizing the pomelo fruit peel, screening; S2 Supercritical CO2 extraction: The entrainer is ethanol: Extraction kettle: Temperature 45 °C, pressure 35 MPa, CO2 flow rate 25 kg / h; Separation kettle I: Temperature 55 °C, pressure 8 Mpa; Separation kettle II: Temperature 32 °C, pressure 5 Mpa; S3 Post-treatment: Combining the products of separation kettle II, rotary evaporation to recover ethanol, and then freeze-drying.

7. The skin care micro-formulation according to claim 1, characterized in that, The Kigelia africana fruit extract is an extract after enzymatic hydrolysis.

8. The skin care micro-formulation according to claim 1, wherein The Crambe abyssinica leaf extract is a subcritical water extract.

9. Use of the skin care micro-formulation according to any one of claims 1 - 8 in the preparation of a topical skin agent.

10. The use according to claim 9 in the preparation of a topical skin agent, characterized in that The described skin care micro-formulation has the effects of soothing and repairing the skin.

Images