Anti-dandruff and anti-alopecia plant composition as well as preparation method and application thereof

Abstract

The invention relates to the technical field of skin care product raw materials, and particularly discloses an anti-dandruff and anti-alopecia plant composition as well as a preparation method and application thereof. The preparation method comprises the following steps: 1) crushing the cacumen biotae, the vitex trifolia fruit, the rhizoma acori graminei, the rhizoma drynariae splendens, the fructus cnidii, the cardiospermum halicacabum, the fructus kochiae, the cockscomb and the herba schizonepetae, and uniformly mixing to obtain a plant mixture; 2) adding water and a compound enzyme into the plant mixture, uniformly mixing, performing enzymolysis at 45-55 DEG C for 6-8 hours, and inactivating to obtain an enzymolysis mixture; (3) adding compound zymophyte into the enzymolysis mixture, fermenting at 27-29 DEG C for 12-24 hours, sterilizing, centrifuging, taking supernate, and concentrating the supernate to obtain plant fermentation liquor; 4, ethyl alcohol is added into the plant fermentation liquid for extraction, filtration and alcohol removal, then glycerol is added for extraction and filtration, a preservative is added, and the anti-dandruff and anti-hair-loss plant composition is obtained.The anti-dandruff and anti-hair-loss plant composition has the advantage that the anti-hair-loss effect of a hair washing product is further improved.

Description

Technical Field

[0001] The present invention relates to the field of skin care product raw materials, and particularly relates to an anti-dandruff and anti-hair loss plant composition, a preparation method thereof and an application thereof. Background Art

[0002] With the acceleration of the pace of life and the continuous increase in work intensity, the number of people troubled by hair loss is increasing, and it shows a trend of getting younger. Hair loss brings huge mental pressure and psychological burden to patients, and thus seriously affects the quality of life.

[0003] In order to alleviate the problem of hair loss, plant extracts with anti-hair loss effects are added to existing shampoo products. However, due to the limitation of their extraction processes, the effects of existing plant extracts are relatively ordinary, resulting in poor anti-hair loss effects of shampoo products and being difficult to meet the needs of consumers. Therefore, there is still room for improvement. Summary of the Invention

[0004] In order to further improve the anti-hair loss effect of shampoo products, the present application provides an anti-dandruff and anti-hair loss plant composition, a preparation method thereof and an application thereof.

[0005] In the first aspect, the present application provides a preparation method of an anti-dandruff and anti-hair loss plant composition, adopting the following technical scheme: A preparation method of an anti-dandruff and anti-hair loss plant composition includes the following steps: Step 1), pulverize and mix together Platycladus orientalis leaves, Vitex trifolia L. var. simplicifolia Cham. fruits, Acorus tatarinowii Schott, Drynaria baronii (Christ) Diels, Cnidium monnieri (L.) Cuss., Cardiospermum halicacabum L., Kochia scoparia (L.) Schrad., Celosia cristata L., and Schizonepeta tenuifolia Briq. to obtain a plant mixture; Step 2), add water and a complex enzyme to the plant mixture, mix evenly, and carry out enzymatic hydrolysis at 45 - 55 °C for 6 - 8 h, and obtain an enzymatically hydrolyzed mixture after inactivation; Step 3), add a complex fermentation bacterium to the enzymatically hydrolyzed mixture, ferment at 27 - 29 °C for 12 - 24 h, carry out sterilization and then centrifugation, take the supernatant, and concentrate the supernatant to obtain a plant fermentation broth; Step 4), add ethanol to the plant fermentation broth for extraction, filter and remove the alcohol, then add glycerol for extraction, filter, and add a preservative to obtain an anti-dandruff and anti-hair loss plant composition.

[0006] By adopting the above technical scheme, through the compounding of Platycladus orientalis leaves, Vitex trifolia L. var. simplicifolia Cham. fruits, Acorus tatarinowii Schott, Drynaria baronii (Christ) Diels, Cnidium monnieri (L.) Cuss., Cardiospermum halicacabum L., Kochia scoparia (L.) Schrad., Celosia cristata L., and Schizonepeta tenuifolia Briq., and then through the extraction by enzymatic hydrolysis and fermentation processes, the content of active ingredients in the obtained anti-dandruff and anti-hair loss plant composition is relatively high. With the mutual cooperation of various active ingredients, it can play a significant role in inhibiting dandruff and preventing hair loss, and can well meet the needs of consumers.

[0007] Preferably, in the step 1), the mass ratio of Platycladus orientalis leaves, Vitex trifolia fruits, Acorus tatarinowii, Davallia mariesii, Cnidium monnieri, Cardiospermum halicacabum, Kochia scoparia, Celosia cristata, and Schizonepeta tenuifolia is 5.5 - 6.5: 1.5 - 2.5: 1.5 - 2.5: 1.5 - 2.5: 1.5 - 2.5: 1.5 - 2.5: 1 - 2: 1 - 2: 0.5 - 1.5.

[0008] By adopting the above technical solution, by specifically selecting the mass ratio of Platycladus orientalis leaves, Vitex trifolia fruits, Acorus tatarinowii, Davallia mariesii, Cnidium monnieri, Cardiospermum halicacabum, Kochia scoparia, Celosia cristata, and Schizonepeta tenuifolia, the active ingredients can be better combined in a specific ratio, so as to exert a better synergistic effect, and further make the anti-dandruff and anti-hair loss effects better.

[0009] Preferably, in the step 2), the complex enzyme is a compound of papain, pectinase, and cellulase.

[0010] By adopting the above technical solution, by specifically selecting the complex enzyme as a compound of papain, pectinase, and cellulase, the enzymatic hydrolysis effect is better, the extraction efficiency is higher, the content of active ingredients in the anti-dandruff and anti-hair loss plant composition is higher, and the anti-dandruff and anti-hair loss effects are better.

[0011] Preferably, in the step 2), the mass ratio of the plant mixture, papain, pectinase, and cellulase is 100: 0.15 - 0.25: 0.25 - 0.35: 0.15 - 0.25.

[0012] By adopting the above technical solution, by specifically selecting the mass ratio of the plant mixture, papain, pectinase, and cellulase, the enzymatic hydrolysis effect can be further improved, and the anti-dandruff and anti-hair loss effects of the composition are better.

[0013] Preferably, in the step 3), the complex fermentation bacteria are a compound of Cryptococcus laurentii yeast liquid, Bacillus subtilis liquid, and Pichia pastoris powder liquid, and the OD values of the Cryptococcus laurentii yeast liquid, Bacillus subtilis liquid, and Pichia pastoris powder liquid are all ≥1.

[0014] By adopting the above technical solution, by specifically selecting the Cryptococcus laurentii yeast liquid, Bacillus subtilis liquid, and Pichia pastoris powder liquid, the fermentation effect is better, and the fermentation product can cooperate with the active ingredients, so as to exert a better anti-dandruff and anti-hair loss effect.

[0015] Preferably, in the step 3), the mass ratio of the enzymatic hydrolysis mixture, Cryptococcus laurentii yeast liquid, Bacillus subtilis liquid, and Pichia pastoris powder liquid is 100: 0.04 - 0.06: 0.04 - 0.06: 0.04 - 0.06.

[0016] By adopting the above technical solution, by specifically selecting the mass ratios of the enzymatic hydrolysis mixture, Cryptococcus laurentii liquid, Bacillus subtilis liquid, and Pichia farinosa powder liquid, the fermentation product can be better combined with the active ingredient in a specific ratio, thereby further improving the anti-dandruff and anti-hair loss effects.

[0017] Preferably, the mass ratio of the plant fermentation broth to the enzymatic hydrolysis mixture in step 3) is 0.19 - 0.21:1.

[0018] By adopting the above technical solution, the content of the active ingredient in the concentrated plant fermentation broth is just right, the process difficulty of adding it to hair care products is relatively low, it can be better combined with the formula of hair care products, and can better enhance the anti-dandruff and anti-hair loss effects of hair care products.

[0019] Preferably, in step 4), the mass ratio of the plant fermentation broth, glycerol, and preservative is 57.9 - 60.1:39 - 41:0.9 - 1.1.

[0020] By adopting the above technical solution, the anti-dandruff and anti-hair loss plant composition has high stability, is not easy to deteriorate, and has good quality.

[0021] In a second aspect, the present application provides an anti-dandruff and anti-hair loss plant composition, adopting the following technical solution: An anti-dandruff and anti-hair loss plant composition, which is prepared by the preparation method of the above-mentioned anti-dandruff and anti-hair loss plant composition.

[0022] By adopting the above technical solution, the anti-dandruff and anti-hair loss plant composition has good effects of inhibiting dandruff and preventing hair loss, has a relatively fast effect, and can well meet the needs of consumers.

[0023] In a third aspect, the present application provides an application of an anti-dandruff and anti-hair loss plant composition, adopting the following technical solution: An application of the above-mentioned anti-dandruff and anti-hair loss plant composition, which is added to hair care products to prepare hair care products with anti-dandruff and anti-hair loss effects.

[0024] By adopting the above technical solution, the hair care products with anti-dandruff and anti-hair loss effects can achieve good effects of inhibiting dandruff and preventing hair loss, and can well meet the needs of consumers.

[0025] In summary, the present application has the following beneficial effects: 1. Since this application selects Platycladus orientalis leaves, Vitex trifolia fruits, Acorus tatarinowii, Davallia mariesii, Cnidium monnieri, Cardiospermum halicacabum, Kochia scoparia, Celosia cristata, and Schizonepeta tenuifolia for compounding, and then extracts them through enzymatic hydrolysis and fermentation processes, the content of active ingredients in the obtained anti-dandruff and anti-hair loss plant composition is relatively high. With the mutual cooperation of various active ingredients, it can significantly inhibit dandruff and prevent hair loss, and can well meet the needs of consumers.

[0026] 2. In this application, it is preferably to specifically select the mass ratios of Platycladus orientalis leaves, Vitex trifolia fruits, Acorus tatarinowii, Davallia mariesii, Cnidium monnieri, Cardiospermum halicacabum, Kochia scoparia, Celosia cristata, and Schizonepeta tenuifolia, so that various active ingredients can be better combined in a specific ratio, thereby playing a better synergistic effect, and further making the anti-dandruff and anti-hair loss effects better.

[0027] 3. In this application, it is preferably to specifically select Cryptococcus laurentii liquid, Bacillus subtilis liquid, and Pichia pastoris powder liquid, so that the fermentation effect is better, and the fermentation products can cooperate with the active ingredients, thereby playing a better anti-dandruff and anti-hair loss effect. Detailed implementation mode

[0028] The following further elaborates on this application in conjunction with the embodiments.

[0029] Example 1 A preparation method of an anti-dandruff and anti-hair loss plant composition includes the following steps: Step 1), pulverize 5.5 kg of Platycladus orientalis leaves, 1.5 kg of Vitex trifolia fruits, 1.5 kg of Acorus tatarinowii, 1.5 kg of Davallia mariesii, 1.5 kg of Cnidium monnieri, 1.5 kg of Cardiospermum halicacabum, 1 kg of Kochia scoparia, 1 kg of Celosia cristata, and 0.5 kg of Schizonepeta tenuifolia through a 40-mesh sieve, and then put them into a stirring kettle, with a rotation speed of 200 r / min, stir for 10 min, and mix evenly to obtain a plant mixture.

[0030] Step 2), take 10 kg of the plant mixture and put it into a reaction kettle, then add 50 kg of water, 0.015 kg of papain, 0.025 kg of pectinase, and 0.015 kg of cellulase, with a rotation speed of 100 r / min, stir for 3 min, and mix evenly. Then heat and keep the temperature at 45 °C, with a rotation speed of 60 r / min, continuously stir for enzymatic hydrolysis for 6 h, and then heat to 85 °C to inactivate for 1 h to obtain an enzymatically hydrolyzed mixture.

[0031] Step 3): Put 10 kg of the enzymatic hydrolysis mixture into a fermentation tank, then add 0.004 kg of Cryptococcus laurentii yeast solution, 0.004 kg of Bacillus subtilis bacterial solution, and 0.004 kg of powdered Pichia yeast solution. Keep the temperature at 27°C and continuously introduce sterile air. Ferment for 12 h, then transfer it to an autoclave, sterilize at a constant temperature of 121°C for 20 min. After cooling to room temperature, transfer it to a centrifuge, centrifuge at 4000 r / min for 30 min, take the supernatant, heat the supernatant to boiling, and concentrate to obtain 1.9 kg of plant fermentation broth; Step 4): Add 579 g of ethanol to 579 g of the plant fermentation broth, keep the temperature at 60°C, stir at a speed of 60 r / min for 6 h, cool to room temperature, filter through a filter cloth, take the filtrate, then heat the filtrate to 80°C to remove ethanol, then add 390 g of glycerol, keep the temperature at 82°C, stir at a speed of 100 r / min for 1 h, cool to room temperature, filter through a 0.1 μm ceramic membrane, take the filtrate, then add 9 g of preservative, stir at a speed of 60 r / min for 5 min, and mix evenly to obtain an anti-dandruff and anti-hair loss plant composition.

[0032] Platycladus orientalis leaves, Vitex trifolia var. simplicifolia fruits, Acorus tatarinowii, Davallia mariesii, Cnidium monnieri, Cardiospermum halicacabum, Kochia scoparia, Celosia cristata, and Schizonepeta tenuifolia are all sun-dried Chinese medicinal materials, and they are all purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd.

[0033] Papain is purchased from Guangdong Mingtong Biotechnology Co., Ltd.

[0034] Pectinase is purchased from Guangzhou Huayu Biotechnology Co., Ltd.

[0035] Cellulase is purchased from Guangdong Mingtong Biotechnology Co., Ltd.

[0036] Cryptococcus laurentii yeast solution is cultured from Cryptococcus laurentii in a liquid medium.

[0037] Cryptococcus laurentii is purchased from Shanghai Collection of Biotechnology Center, and the strain number is SHBCC D21253 The preparation method of Cryptococcus laurentii yeast solution is as follows: Mix 1 g of glucose, 0.5 g of malt extract, 0.0135 g of citric acid, 0.126 g of sodium citrate, and 98.3605 g of water evenly, then inoculate 1 mg of Cryptococcus laurentii strain, and culture at a constant temperature of 28°C until the OD value of the bacterial solution = 1.

[0038] Glucose is purchased from Zibo Ruixue Sugar Industry Co., Ltd.

[0039] Malt extract is purchased from Shaanxi Chenming Biotechnology Co., Ltd.

[0040] Citric acid is purchased from Henan Juteng Chemical Products Co., Ltd.

[0041] Sodium citrate was purchased from Jiangsu Yuanyuan Biotechnology Co., Ltd.

[0042] The Bacillus subtilis bacterial liquid was cultured from Bacillus subtilis in a liquid medium.

[0043] Bacillus subtilis was purchased from Shanghai Biological Collection Center, and the strain number is SHBCC D50150.

[0044] The preparation method of the Bacillus subtilis bacterial liquid is as follows: Mix 1 g of peptone, 0.5 g of yeast extract, 1 g of sodium chloride, and 97.5 g of water evenly, then inoculate 1 mg of Bacillus subtilis and culture at a constant temperature of 30 °C until the OD value of the bacterial liquid = 1.

[0045] Peptone was purchased from Hubei Haijia Biotechnology Co., Ltd.

[0046] Yeast extract was purchased from Guangzhou Huayu Biotechnology Co., Ltd.

[0047] Sodium chloride was purchased from Jinan Ruilin Chemical Co., Ltd.

[0048] The Pichia pastoris powder bacterial liquid was cultured from Pichia pastoris powder in a liquid medium.

[0049] Pichia pastoris powder was purchased from Shanghai Biological Collection Center, and the strain number is SHBCC D21661.

[0050] The preparation method of the Pichia pastoris powder bacterial liquid is as follows: Mix 1 g of glucose, 0.5 g of peptone, 0.3 g of yeast extract, 0.3 g of malt extract, 0.0135 g of citric acid, 0.0126 g of sodium citrate, and 97.8739 g of water evenly, then inoculate 1 mg of Pichia pastoris powder and culture at a constant temperature of 28 °C until the OD value of the bacterial liquid = 1.

[0051] Glucose was purchased from Zibo Ruixue Sugar Industry Co., Ltd.

[0052] Peptone was purchased from Hubei Haijia Biotechnology Co., Ltd.

[0053] Yeast extract was purchased from Guangzhou Huayu Biotechnology Co., Ltd.

[0054] Malt extract was purchased from Shaanxi Chenming Biotechnology Co., Ltd.

[0055] Citric acid was purchased from Henan Juteng Chemical Products Co., Ltd.

[0056] Sodium citrate was purchased from Jiangsu Yuanyuan Biotechnology Co., Ltd.

[0057] The preservative is a compound of 1,2 - hexanediol and p - hydroxyacetophenone, and the mass ratio of 1,2 - hexanediol to p - hydroxyacetophenone is 1:1.

[0058] 1,2 - hexanediol was purchased from Hubei Xinghengye Technology Co., Ltd.

[0059] p - hydroxyacetophenone was purchased from Wuhan Huaxiang Kejie Biotechnology Co., Ltd.

[0060] Example 2 A preparation method of an anti - dandruff and anti - hair loss plant composition, comprising the following steps: Step 1), 6 kg of Platycladus orientalis leaves, 2 kg of Vitex trifolia var. simplicifolia fruits, 2 kg of Acorus tatarinowii, 2 kg of Davallia mariesii, 2 kg of Cnidium monnieri, 2 kg of Cardiospermum halicacabum, 1.5 kg of Kochia scoparia, 1.5 kg of Celosia cristata, and 1 kg of Schizonepeta tenuifolia were crushed through a 40 - mesh sieve, then put into a stirring kettle, with a rotation speed of 200 r / min, stirred for 10 min, and mixed evenly to obtain a plant mixture.

[0061] Step 2), 10 kg of the plant mixture was put into a reaction kettle, then 50 kg of water, 0.02 kg of papain, 0.03 kg of pectinase, and 0.02 kg of cellulase were added, with a rotation speed of 100 r / min, stirred for 3 min, and mixed evenly. Then it was heated and kept at a constant temperature of 50 °C, with a rotation speed of 60 r / min, continuously stirred for enzymatic hydrolysis for 6 - 8 h, and then heated to 86 °C for 1 h to inactivate the enzymes, obtaining an enzymolysis mixture.

[0062] Step 3), 10 kg of the enzymolysis mixture was put into a fermentation tank, then 0.005 kg of Cryptococcus laurentii yeast liquid, 0.005 kg of Bacillus subtilis bacterial liquid, and 0.005 kg of Pichia pastoris powder yeast liquid were added, kept at a constant temperature of 28 °C, continuously aerated with sterile air, fermented for 18 h, then transferred to an autoclave, sterilized at a constant temperature of 121 °C for 20 min, cooled to room temperature, transferred to a centrifuge, centrifuged at 4000 r / min for 30 min, the supernatant was taken, the supernatant was heated to boiling, concentrated to obtain 2 kg of plant fermentation broth; Step 4), 590 g of the plant fermentation broth was added with 590 g of ethanol, kept at a constant temperature of 60 °C, with a rotation speed of 60 r / min, stirred and extracted for 7 h, cooled to room temperature, filtered through a filter cloth, the filtrate was taken, then the filtrate was heated to 80 °C to remove ethanol, then 400 g of glycerol was added, kept at a constant temperature of 84 °C, with a rotation speed of 100 r / min, stirred and extracted for 1.5 h, cooled to room temperature, filtered through a 0.1 - μm ceramic membrane, the filtrate was taken, then 10 g of preservative was added, with a rotation speed of 60 r / min, stirred for 5 min, and mixed evenly to obtain the anti - dandruff and anti - hair loss plant composition.

[0063] Platycladus orientalis leaves, Vitex trifolia var. simplicifolia fruits, Acorus tatarinowii, Davallia mariesii, Cnidium monnieri, Cardiospermum halicacabum, Kochia scoparia, Celosia cristata, and Schizonepeta tenuifolia are all sun - dried Chinese medicinal materials, and all were purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd.

[0064] Papain was purchased from Guangdong Mingtong Biotechnology Co., Ltd.

[0065] Pectinase was purchased from Guangzhou Huayu Biotechnology Co., Ltd.

[0066] Cellulase was purchased from Guangdong Mingtong Biotechnology Co., Ltd.

[0067] Cryptococcus laurentii yeast solution was cultured from Cryptococcus laurentii in a liquid medium.

[0068] Cryptococcus laurentii was purchased from Shanghai Collection Center of Biotechnology, and the strain number is SHBCC D21253. The preparation method of Cryptococcus laurentii yeast solution is as follows: Mix 1 g of glucose, 0.5 g of malt extract, 0.0135 g of citric acid, 0.126 g of sodium citrate, and 98.3605 g of water evenly, then inoculate 1 mg of Cryptococcus laurentii strain, and culture at a constant temperature of 28 °C until the OD value of the bacterial solution = 1.

[0069] Glucose was purchased from Zibo Ruixue Sugar Industry Co., Ltd.

[0070] Malt extract was purchased from Shaanxi Chenming Biotechnology Co., Ltd.

[0071] Citric acid was purchased from Henan Juteng Chemical Products Co., Ltd.

[0072] Sodium citrate was purchased from Jiangsu Yuanyuan Biotechnology Co., Ltd.

[0073] Bacillus subtilis yeast solution was cultured from Bacillus subtilis in a liquid medium.

[0074] Bacillus subtilis was purchased from Shanghai Collection Center of Biotechnology, and the strain number is SHBCC D50150.

[0075] The preparation method of Bacillus subtilis yeast solution is as follows: Mix 1 g of peptone, 0.5 g of yeast extract, 1 g of sodium chloride, and 97.5 g of water evenly, then inoculate 1 mg of Bacillus subtilis, and culture at a constant temperature of 30 °C until the OD value of the bacterial solution = 1.

[0076] Peptone was purchased from Hubei Haijia Biotechnology Co., Ltd.

[0077] Yeast extract was purchased from Guangzhou Huayu Biotechnology Co., Ltd.

[0078] Sodium chloride was purchased from Jinan Ruilin Chemical Co., Ltd.

[0079] Pichia pastoris powder yeast solution was cultured from Pichia pastoris powder in a liquid medium.

[0080] Pichia farinosa was purchased from Shanghai Biological Collection Center, and the strain number is SHBCC D21661.

[0081] The preparation method of the Pichia farinosa yeast solution is as follows: Mix 1 g of glucose, 0.5 g of peptone, 0.3 g of yeast extract, 0.3 g of malt extract, 0.0135 g of citric acid, 0.0126 g of sodium citrate, and 97.8739 g of water evenly, then inoculate 1 mg of Pichia farinosa, and cultivate at a constant temperature of 28 °C until the OD value of the yeast solution = 1.

[0082] Glucose was purchased from Zibo Ruixue Sugar Industry Co., Ltd.

[0083] Peptone was purchased from Hubei Haijia Biotechnology Co., Ltd.

[0084] Yeast extract was purchased from Guangzhou Huayu Biotechnology Co., Ltd.

[0085] Malt extract was purchased from Shaanxi Chenming Biotechnology Co., Ltd.

[0086] Citric acid was purchased from Henan Juteng Chemical Products Co., Ltd.

[0087] Sodium citrate was purchased from Jiangsu Yuanyuan Biotechnology Co., Ltd.

[0088] The preservative is a compound of 1,2 - hexanediol and p - hydroxyacetophenone, and the mass ratio of 1,2 - hexanediol to p - hydroxyacetophenone is 1:1.

[0089] 1,2 - Hexanediol was purchased from Hubei Xinghengye Technology Co., Ltd.

[0090] p - Hydroxyacetophenone was purchased from Wuhan Huaxiang Kejie Biotechnology Co., Ltd.

[0091] Example 3 A preparation method of an anti - dandruff and anti - hair loss plant composition, comprising the following steps: Step 1), pulverize 6.5 kg of Platycladus orientalis leaves, 2.5 kg of Vitex trifolia fruits, 2.5 kg of Acorus tatarinowii, 2.5 kg of Davallia mariesii, 2.5 kg of Cnidium monnieri, 2.5 kg of Cardiospermum halicacabum, 2 kg of Kochia scoparia, 2 kg of Celosia cristata, and 1.5 kg of Schizonepeta tenuifolia through a 40 - mesh sieve, then put them into a stirring kettle, stir at a speed of 200 r / min for 10 min to mix evenly to obtain a plant mixture.

[0092] Step 2): Put 10 kg of the plant mixture into the reaction kettle, then add 50 kg of water, 0.025 kg of papain, 0.035 kg of pectinase, and 0.025 kg of cellulase. Stir at a speed of 100 r / min for 3 min to mix evenly. Then heat and keep the temperature at 55 °C, stir at a speed of 60 r / min, and continuously stir for enzymatic hydrolysis for 8 h. Then heat to 88 °C to inactivate for 1 h to obtain the enzymatically hydrolyzed mixture.

[0093] Step 3): Put 10 kg of the enzymatically hydrolyzed mixture into the fermentation tank, then add 0.006 kg of Cryptococcus laurentii yeast liquid, 0.006 kg of Bacillus subtilis bacterial liquid, and 0.006 kg of Pichia pastoris powder yeast liquid. Keep the temperature at 29 °C, continuously introduce sterile air, and ferment for 24 h. Then transfer it to the autoclave, sterilize at a constant temperature of 121 °C for 20 min. After cooling to room temperature, transfer it to a centrifuge, centrifuge at 4000 r / min for 30 min, take the supernatant, heat the supernatant to boiling, and concentrate to obtain 2.1 kg of plant fermentation broth; Step 4): Add 601 g of ethanol to 601 g of the plant fermentation broth, keep the temperature at 60 °C, stir at a speed of 60 r / min, and extract for 8 h. Cool to room temperature, filter through a filter cloth, take the filtrate, then heat the filtrate to 80 °C to remove ethanol, then add 410 g of glycerol, keep the temperature at 86 °C, stir at a speed of 100 r / min, and extract for 2 h. After cooling to room temperature, filter through a 0.1 μm ceramic membrane, take the filtrate, then add 11 g of preservative, stir at a speed of 60 r / min for 5 min to mix evenly to obtain the anti-dandruff and anti-hair loss plant composition.

[0094] Platycladus orientalis leaves, Vitex trifolia L. var. simplicifolia Cham. fruits, Acorus tatarinowii Schott, Drynaria baronii (Christ) Diels, Cnidium monnieri (L.) Cuss., Cardiospermum halicacabum L., Kochia scoparia (L.) Schrad., Celosia cristata L., and Schizonepeta tenuifolia Briq. are all sun-dried Chinese medicinal materials, and they are all purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd.

[0095] Papain is purchased from Guangdong Mingtong Biotechnology Co., Ltd.

[0096] Pectinase is purchased from Guangzhou Huayu Biotechnology Co., Ltd.

[0097] Cellulase is purchased from Guangdong Mingtong Biotechnology Co., Ltd.

[0098] Cryptococcus laurentii yeast liquid is prepared by culturing Cryptococcus laurentii in a liquid medium.

[0099] Cryptococcus laurentii is purchased from Shanghai Biological Collection Center, and the strain number is SHBCC D21253 The preparation method of Cryptococcus laurentii yeast liquid is as follows: Mix 1 g of glucose, 0.5 g of malt extract, 0.0135 g of citric acid, 0.126 g of sodium citrate, and 98.3605 g of water evenly, then inoculate 1 mg of Cryptococcus laurentii strain, and culture at a constant temperature of 28 °C until the OD value of the bacterial liquid = 1.

[0100] The glucose was purchased from Zibo Ruixue Sugar Industry Co., Ltd.

[0101] The malt extract was purchased from Shaanxi Chenming Biotechnology Co., Ltd.

[0102] The citric acid was purchased from Henan Juteng Chemical Products Co., Ltd.

[0103] The sodium citrate was purchased from Jiangsu Yuanyuan Biotechnology Co., Ltd.

[0104] The Bacillus subtilis bacterial liquid was prepared by culturing Bacillus subtilis in a liquid medium.

[0105] The Bacillus subtilis was purchased from Shanghai Biological Collection Center, and the strain number is SHBCC D50150.

[0106] The preparation method of the Bacillus subtilis bacterial liquid is as follows: Mix 1 g of peptone, 0.5 g of yeast extract, 1 g of sodium chloride, and 97.5 g of water evenly, then inoculate 1 mg of Bacillus subtilis, and culture at a constant temperature of 30 °C until the OD value of the bacterial liquid = 1.

[0107] The peptone was purchased from Hubei Haijia Biotechnology Co., Ltd.

[0108] The yeast extract was purchased from Guangzhou Huayu Biotechnology Co., Ltd.

[0109] The sodium chloride was purchased from Jinan Ruilin Chemical Co., Ltd.

[0110] The Pichia pastoris powder bacterial liquid was prepared by culturing Pichia pastoris powder in a liquid medium.

[0111] The Pichia pastoris powder was purchased from Shanghai Biological Collection Center, and the strain number is SHBCC D21661.

[0112] The preparation method of the Pichia pastoris powder bacterial liquid is as follows: Mix 1 g of glucose, 0.5 g of peptone, 0.3 g of yeast extract, 0.3 g of malt extract, 0.0135 g of citric acid, 0.0126 g of sodium citrate, and 97.8739 g of water evenly, then inoculate 1 mg of Pichia pastoris powder, and culture at a constant temperature of 28 °C until the OD value of the bacterial liquid = 1.

[0113] The glucose was purchased from Zibo Ruixue Sugar Industry Co., Ltd.

[0114] The peptone was purchased from Hubei Haijia Biotechnology Co., Ltd.

[0115] The yeast extract was purchased from Guangzhou Huayu Biotechnology Co., Ltd.

[0116] The malt extract was purchased from Shaanxi Chenming Biotechnology Co., Ltd.

[0117] The citric acid was purchased from Henan Juteng Chemical Products Co., Ltd.

[0118] The sodium citrate was purchased from Jiangsu Yuanyuan Biotechnology Co., Ltd.

[0119] The preservative is a compound of 1,2 - hexanediol and p - hydroxyacetophenone, and the mass ratio of 1,2 - hexanediol to p - hydroxyacetophenone is 1:1.

[0120] The 1,2 - hexanediol was purchased from Hubei Xinghengye Technology Co., Ltd.

[0121] The p - hydroxyacetophenone was purchased from Wuhan Huaxiang Kejie Biotechnology Co., Ltd.

[0122] Comparative Example 1 A preparation method of an anti - dandruff and anti - hair - loss plant composition, compared with Example 2, the difference is only that: Polygonum multiflorum was used to replace Platycladus orientalis leaves in equal amount, Astragalus membranaceus was used to replace Vitex trifolia fruits in equal amount, Ligustrum lucidum was used to replace Acorus tatarinowii in equal amount, Cuscuta chinensis was used to replace Drynaria baronii in equal amount, Zingiber officinale root was used to replace Cnidium monnieri in equal amount, Panax ginseng was used to replace Cardiospermum halicacabum in equal amount, Angelica sinensis was used to replace Kochia scoparia in equal amount, Anemarrhena asphodeloides was used to replace Celosia cristata in equal amount, and Swertia bimaculata was used to replace Schizonepeta tenuifolia in equal amount.

[0123] The Polygonum multiflorum was purchased from Bozhou Yirentang Pharmaceutical Co., Ltd.

[0124] The Astragalus membranaceus was purchased from Bozhou Yirentang Pharmaceutical Co., Ltd.

[0125] The Ligustrum lucidum was purchased from Bozhou Ruanantang Pharmaceutical Co., Ltd.

[0126] The Cuscuta chinensis was purchased from Bozhou Ruanantang Pharmaceutical Co., Ltd.

[0127] The Zingiber officinale root was purchased from Bozhou Huozhengtang Pharmaceutical Co., Ltd.

[0128] The Panax ginseng was purchased from Bozhou Fuyongtang Pharmaceutical Co., Ltd.

[0129] The Angelica sinensis was purchased from Bozhou Yongzheng Pharmaceutical Co., Ltd.

[0130] The Anemarrhena asphodeloides was purchased from Bozhou Yongzheng Pharmaceutical Co., Ltd.

[0131] The Swertia bimaculata was purchased from Bozhou Baicaokang Health Industry Co., Ltd.

[0132] Comparative Example 2 A preparation method of an anti-dandruff and anti-hair loss plant composition, compared with Example 2, the difference is only that: Candida gelida is used to replace Cryptococcus laurentii in equal amount.

[0133] Candida gelida was purchased from Shanghai Collection of Biotechnology, and the strain number is SHBCC D20804.

[0134] Comparative Example 3 A preparation method of an anti-dandruff and anti-hair loss plant composition, compared with Example 2, the difference is only that: Bacillus megaterium is used to replace Bacillus subtilis in equal amount.

[0135] Bacillus megaterium was purchased from Shanghai Collection of Biotechnology, and the strain number is SHBCC D24587.

[0136] Comparative Example 4 A preparation method of an anti-dandruff and anti-hair loss plant composition, compared with Example 2, the difference is only that: Schizosaccharomyces pombe is used to replace Pichia farinosa in equal amount.

[0137] Schizosaccharomyces pombe was purchased from Shanghai Collection of Biotechnology, and the strain number is SHBCC D54083.

[0138] Comparative Example 5 A preparation method of an anti-dandruff and anti-hair loss plant composition, compared with Example 2, the difference is only that: Candida gelida is used to replace Cryptococcus laurentii in equal amount, Bacillus megaterium is used to replace Bacillus subtilis in equal amount, and Schizosaccharomyces pombe is used to replace Pichia farinosa in equal amount.

[0139] Candida gelida was purchased from Shanghai Collection of Biotechnology, and the strain number is SHBCC D20804.

[0140] Bacillus megaterium was purchased from Shanghai Collection of Biotechnology, and the strain number is SHBCC D24587.

[0141] Schizosaccharomyces pombe was purchased from Shanghai Collection of Biotechnology, and the strain number is SHBCC D54083.

[0142] Application Example 1 A shampoo, the preparation method of the shampoo is as follows: Step 01): Add 71.33 kg of water, 15 kg of sodium lauryl ether sulfate, 0.3 kg of sodium lauryl sulfate, 0.1 kg of disodium EDTA, 0.1 kg of allantoin, 1 kg of DEA palm kernel amide, and 0.05 kg of panthenol into a stirring kettle, heat up to 85 °C, stir at 400 r / min for 20 min to obtain a premix.

[0143] Step 02): Add 0.25 kg of guar gum hydroxypropyltrimethyl ammonium chloride and 0.02 kg of citric acid to the premix, stir at 100 r / min for 5 min, then cool down to 50 °C, add 3 kg of cocoamidopropyl betaine, 0.8 kg of polyquaternium-7, 2 kg of sodium chloride, 0.5 kg of phenoxyethanol, 0.02 kg of ethylhexylglycerin, 0.35 kg of sodium benzoate, and 5 kg of anti-dandruff and anti-hair loss plant composition, stir at 200 r / min for 15 min until evenly mixed to obtain a shampoo.

[0144] The anti-dandruff and anti-hair loss plant composition is the anti-dandruff and anti-hair loss plant composition prepared in Example 1.

[0145] Experiment 1 Dilute the anti-dandruff and anti-hair loss plant compositions of each example and comparative example with deionized water to prepare test samples.

[0146] Preparation of test samples: Mix 5 g of anti-dandruff and anti-hair loss plant composition and 95 g of deionized water evenly to obtain test samples.

[0147] Detect the 5-α reductase inhibition rate (%) and Malassezia inhibition rate (%) of the test samples.

[0148] Detection method for 5-α reductase inhibition rate: I. Test purpose and principle From the perspective of structure-activity relationship, 5α-reductase is a membrane protein located on the microsomes of target cells, relying on reduced coenzyme II (NADPH) as a hydrogen donor. This enzyme is widely present in the prostate and other tissues and organs, and is a catalyst for the conversion of testosterone to dihydrotestosterone. It irreversibly catalyzes testosterone (T) into dihydrotestosterone (DHT) with stronger physiological activity. First, establish an enzymatic reaction system, taking advantage of the spontaneous fluorescence property of reduced coenzyme II NADPH (NADPH itself has strong fluorescence, and when NADPH reacts with the reducing agent and substrate, NADPH generates NADP+ and the fluorescence quenches accordingly).

[0149] It is mainly characterized by evaluating the inhibition rate of the test sample on 5α-reductase. 5α-reductase is an important androgen-metabolizing enzyme in the skin, which can irreversibly convert testosterone (T) into dihydrotestosterone (DHT). DHT is the most active androgen and is a key factor leading to androgenetic alopecia. By inhibiting the metabolism of 5α-reductase and controlling the formation of dihydrotestosterone, the anti-hair loss effect can be achieved.

[0150] II. Test indicators The 5α-reductase inhibition rate of the sample is higher than that of the blank group and shows a significant difference (P < 0.05).

[0151] III. Test methods (1) Add 0.1 mL of the sample solution with the same concentration to each of the sample tube and the sample background. Add 0.5 mL of the enzyme preparation to the sample tube, the sample blank, the enzyme reaction tube, and the enzyme blank respectively.

[0152] (2) Add 0.1 mL of the testosterone solution to the sample tube and the enzyme reaction tube. Add 0.1 mL of the NADPH solution and 2.0 mL of the pH 6.0 phosphate buffer to the sample tube, the sample blank, the enzyme reaction tube, and the enzyme blank respectively; incubate in a constant temperature water bath at 37°C for 1 h.

[0153] (3) Add the pH 6.0 phosphate buffer to the sample tube, the sample blank, the enzyme reaction tube, and the enzyme blank respectively, and make up the volume to 5 mL; centrifuge at 12000 r / min for 5 min. After taking the supernatant, filter it with a 0.45 μm filter membrane. (4) Transfer each reaction solution into a 1 cm cuvette and measure the absorbance value at 340 nm.

[0154] Method for detecting the antibacterial rate of Malassezia: I. Test principle Seborrheic dermatitis is a chronic inflammation occurring on the basis of seborrhea. Seborrheic dermatitis occurring on the head is mainly manifested by dandruff and itching, and is a common skin disorder. Malassezia is a conditional pathogenic lipophilic fungus on the surface of the human skin, which is closely related to the onset of seborrheic dermatitis and is an important factor leading to seborrheic dermatitis. In this test, the inhibition rate of the test sample on Malassezia furfur is measured. When the antibacterial rate > 50% - 90%, it indicates that the test sample has an inhibitory effect on Malassezia furfur. When the antibacterial rate > 90%, it indicates that the test sample has a strong inhibitory effect on Malassezia furfur, indicating that the sample has a certain anti-dandruff effect under this condition.

[0155] II. Test methods 1. Preparation of bacterial suspension: Take the culture of the 5th generation of bacteria. Use a pipette to suck 5 mL of PBS diluent into a petri dish, and wash the bacterial lawn with a spreading rod. Subsequently, use a pipette to transfer the washing liquid to another sterile test tube, shake well to make a bacterial suspension. Use a micropipette to drop 10 μL of the bacterial suspension onto a sterilized degreased white muslin cloth piece (10 mm x 10 mm), and dry it at 36°C ± 1°C or air-dry it at room temperature for standby. (The required concentration is between 1.0X104 CFU / piece and 9.0X104 CFU / piece).

[0156] 2. Weigh the sample at a dose of 5 g / piece into a sterile petri dish, and place it in a water bath at 20°C ± 1°C for 5 min.

[0157] 3. Use sterile forceps to pick up the bacteria-infected carrier, immerse the carrier completely in the sample, and start timing immediately. Wait for the bacteria-infected carrier and the sample to interact for a certain period of time. Take the bacteria-infected carrier and add it to a 5.0 mL PBS test tube to mix, place it on a vortex oscillator and shake for 1 min to wash down the test bacteria. Pipette 1.0 mL of the test bacteria and sample mixture and inoculate 2 petri dishes, and pour the culture medium. When the number of bacteria cannot be counted, make 10-fold serial dilutions with PBS, select appropriate dilution degrees, pipette 1.0 mL respectively and inoculate 2 petri dishes for viable bacteria culture and counting.

[0158] 4. At the same time, use PBS instead of the sample to conduct a parallel test as a positive control. The number of recovered colonies in the positive control is between 1.0X104 CFU / piece and 9.0X104 CFU / piece. Take the same batch of PBS and culture medium as negative controls. All test samples and control samples are cultured at (36 ± 1)°C for 3 d to observe the final results. The test is repeated 3 times to calculate the antibacterial rate.

[0159] The specific experimental data of Experiment 1 are shown in Table 1.

[0160] Table 1 5-α Reductase Inhibition Rate (%) Malassezia Bacteriostatic Rate (%) Example 1 53.661 63.16 Example 2 54.153 63.72 Example 3 54.467 64.08 Comparative Example 1 42.347 39.56 Comparative Example 2 47.653 55.33 Comparative Example 3 45.341 51.19 Comparative Example 4 46.668 53.37 Comparative Example 5 44.125 49.88 According to the data comparison of each example and comparative example in Table 1, the 5-α reductase inhibition rate and Malassezia antibacterial rate of each example are significantly higher than those of each comparative example. It can be seen that the anti-hair loss and anti-dandruff effects of each example are significantly better than those of each comparative example. Each comparative example uses other plants with anti-hair loss effects as raw materials for extraction and uses other fermentation bacteria for fermentation, which all lead to a decrease in the anti-hair loss and anti-dandruff effects.

[0161] It can be seen that the special compounding of Platycladus orientalis leaves, Vitex trifolia fruits, Acorus tatarinowii, Drynaria baronii, Cnidium monnieri, Cardiospermum halicacabum, Kochia scoparia, Celosia cristata, and Schizonepeta tenuifolia in each example, combined with fermentation by Cryptococcus laurentii, Bacillus subtilis, and Pichia farinosa, can make the obtained extract play a more significant anti-hair loss and anti-dandruff effect and better meet the needs of consumers.

[0162] This specific embodiment is only an interpretation of the present application and does not limit the present application. After reading this specification, those skilled in the art can make modifications to this embodiment without creative contributions as needed, but as long as it is within the scope of the claims of the present application, it is protected by the patent law.

Claims

1. A preparation method of an anti-dandruff and anti-hair loss plant composition, characterized in that: It includes the following steps: Step 1), pulverize and mix Platycladus orientalis leaves, Vitex trifolia L. var. simplicifolia Cham., Acorus tatarinowii Schott, Davallia mariesii Moore ex Bak., Fructus cnidii, Cardiospermum halicacabum L., Kochia scoparia (L.) Schrad., Celosia cristata L., and Schizonepeta tenuifolia Briq. evenly to obtain a plant mixture; Step 2), add water and a complex enzyme to the plant mixture, mix evenly, and carry out enzymatic hydrolysis at 45 - 55 °C for 6 - 8 h. After inactivation, an enzymolysis mixture is obtained; Step 3), add a complex fermentation bacterium to the enzymolysis mixture, ferment at 27 - 29 °C for 12 - 24 h, sterilize, centrifuge, take the supernatant, and concentrate the supernatant to obtain a plant fermentation broth; Step 4), add ethanol to the plant fermentation broth for extraction, filter and remove alcohol, then add glycerol for extraction, filter, and add a preservative to obtain an anti-dandruff and anti-hair loss plant composition.

2. The preparation method of an anti-dandruff and anti-hair loss plant composition according to claim 1, wherein: In the said Step 1), the mass ratio of Platycladus orientalis leaves, Vitex trifolia L. var. simplicifolia Cham., Acorus tatarinowii Schott, Davallia mariesii Moore ex Bak., Fructus cnidii, Cardiospermum halicacabum L., Kochia scoparia (L.) Schrad., Celosia cristata L., and Schizonepeta tenuifolia Briq. is 5.5 - 6.5:1.5 - 2.5:1.5 - 2.5:1.5 - 2.5:1.5 - 2.5:1.5 - 2.5:1 - 2:1 - 2:0.5 - 1.

5.

3. The preparation method of an anti-dandruff and anti-hair loss plant composition according to claim 1, characterized in that: In the said Step 2), the complex enzyme is a compound of papain, pectinase, and cellulase.

4. The preparation method of an anti-dandruff and anti-hair loss plant composition according to claim 3, characterized in that: In the said Step 2), the mass ratio of the plant mixture, papain, pectinase, and cellulase is 100:0.15 - 0.25:0.25 - 0.35:0.15 - 0.

25.

5. The preparation method of an anti-dandruff and anti-hair loss plant composition according to claim 1, characterized in that: In the said Step 3), the complex fermentation bacterium is a compound of Cryptococcus laurentii yeast liquid, Bacillus subtilis liquid, and Pichia pastoris powder liquid, and the OD value of the Cryptococcus laurentii yeast liquid, Bacillus subtilis liquid, and Pichia pastoris powder liquid is ≥1.

6. The preparation method of an anti-dandruff and anti-hair loss plant composition according to claim 5, characterized in that: In the said Step 3), the mass ratio of the enzymolysis mixture, Cryptococcus laurentii yeast liquid, Bacillus subtilis liquid, and Pichia pastoris powder liquid is 100:0.04 - 0.06:0.04 - 0.06:0.04 - 0.

06.

7. The preparation method of an anti-dandruff and anti-hair loss plant composition according to claim 1, characterized in that: The mass ratio of the plant fermentation broth to the enzymolysis mixture in the said Step 3) is 0.19 - 0.21:

1.

8. The preparation method of an anti-dandruff and anti-hair loss plant composition according to claim 7, characterized in that: In the said Step 4), the mass ratio of the plant fermentation broth, glycerol, and preservative is 57.9 - 60.1:39 - 41:0.9 - 1.

1.

9. A dandruff-removing and hair-loss-preventing plant composition, characterized in that: The said anti-dandruff and anti-hair loss plant composition is prepared by the preparation method of the anti-dandruff and anti-hair loss plant composition according to any one of claims 1 - 8.

10. Use of the anti-dandruff and anti-hair loss plant composition according to claim 9, characterized in that: The said anti-dandruff and anti-hair loss plant composition is added to hair care products to prepare hair care products with anti-dandruff and anti-hair loss effects.