Anti-wrinkle composition, application and skin care product

Abstract

The invention belongs to the technical field of skin care, and discloses an anti-wrinkle composition, an application and a skincare product, the anti-wrinkle composition comprises an abelmoschus esculentus extract and an evening primrose root extract, and the abelmoschus esculentus extract and the evening primrose root extract are obtained by compounding and extracting abelmoschus esculentus and evening primrose root according to the weight ratio of (3-7): (3-9); the anti-wrinkle composition disclosed by the invention is obtained by compounding and extracting abelmoschus esculentus, evening primrose root and safflower in a weight ratio of (2-8): (3-9): (5-20). According to the anti-wrinkle extract provided by the invention, the abelmoschus esculentus extract and the evening primrose root extract are compounded, so that the skin anti-wrinkle effect of the composition can be synergistically improved.

Description

Technical Field

[0001] The present invention relates to the technical field of skin care, and particularly relates to an anti-wrinkle composition, its use, and skin care products. Background Art

[0002] Skin anti-wrinkle care not only concerns the rejuvenation of the external image but also the scientific maintenance of skin health. With the growth of age and continuous damage from the external environment (such as ultraviolet rays and free radicals), the skin's collagen gradually loses and its elasticity decreases, leading to the formation of wrinkles; anti-wrinkle care can reduce existing wrinkles and prevent new lines by stimulating collagen regeneration, delaying oxidative aging, and repairing the barrier function, thus maintaining the skin in a firm and plump state; in addition, the smooth and delicate state of the skin is often closely related to personal confidence and quality of life. Therefore, anti-wrinkle is not only a physiological need but also an extension of psychological and social image management.

[0003] Based on this, many skin anti-wrinkle products are active in the market. For example, in the prior art 1: Chinese Patent Application No. 202211469185.1 discloses an anti-wrinkle composition: hydrolyzed collagen, arginine / lysine polypeptide, and ethylbisiminomethylguaiacol manganese chloride. The hydrolyzed collagen can increase the production of collagen and hyaluronic acid, reduce skin laxity, and improve skin elasticity. When used in combination with arginine / lysine polypeptide, it promotes collagen regeneration, and its strong muscle relaxation effect has an immediate anti-wrinkle effect. Ethylbisiminomethylguaiacol manganese chloride has good antioxidant properties, reducing the reduction of skin moisture, elasticity, and firmness caused by skin damage, thereby achieving a firming effect on the skin.

[0004] Also, in the prior art 2: Chinese Patent Application No. 201810802205.X discloses a cosmetic composition with the functions of increasing skin elasticity and anti-wrinkle and its application. The cosmetic composition contains: 0.01% - 1.0% ceramide; 0.001% - 0.1% palmitoyl oligopeptide; 0.05% - 1.0% royal jelly; 0.25% - 5% lecithin. In the present invention, the weight ratio of the royal jelly (calculated on a dry weight basis) to lecithin is 1:5, in the form of an aqueous solution of bilayer micro-liposomes with a particle size of 30nm - 130nm. The cosmetic containing ceramide, palmitoyl oligopeptide, royal jelly, and lecithin in the present invention can achieve various effects such as improving skin wrinkles, increasing skin elasticity, and restoring skin function.

[0005] From the prior art 1 and the prior art 2, it can be seen that compounding multiple components can obtain a cosmetic composition with better anti-wrinkle effects, which is a good research direction; however, due to the increasingly fierce competition in the cosmetics market and the growing diverse demands of consumers for skin care products, in order to enhance the market competitiveness of products, more anti-wrinkle compositions need to be developed to meet the diverse needs of consumers. Summary of the Invention

[0006] One of the objectives of the present invention is to provide an anti-wrinkle composition, which is obtained by compounding Abelmoschus moschatus extract and evening primrose root extract, has excellent skin anti-wrinkle effect, and can meet the needs of consumers.

[0007] Another objective of the present invention is to provide a use of an anti-wrinkle composition, which is used to prepare skin care products and can endow the skin care products with excellent skin anti-wrinkle efficacy.

[0008] Meanwhile, the present invention also provides a skin care product, which provides more skin care options for the market and meets the diverse needs of consumers.

[0009] To achieve the above objectives, the present invention provides an anti-wrinkle composition, which includes Abelmoschus moschatus extract and evening primrose root extract, and the Abelmoschus moschatus extract and evening primrose root extract are obtained by compound extraction of Abelmoschus moschatus and evening primrose root according to a weight ratio of 3-7:3-9.

[0010] The oil-soluble components in Abelmoschus moschatus have strong antioxidant activity, can scavenge free radicals, block ultraviolet-induced oxidative stress, and reduce the oxidative degradation of collagen and elastic fibers; and the unsaturated fatty acids contained therein can supplement the intercellular lipids of epidermal cells, repair the skin barrier, and enhance the water-locking ability.

[0011] The active ingredients contained in evening primrose can inhibit pro-inflammatory enzymes, reduce the release of PGE2, and block the activation of MMPs induced by inflammation; meanwhile, the phytosterols and polyphenolic substances contained in evening primrose root extract can inhibit the formation of advanced glycation end products, prevent the cross-linking and hardening of collagen fibers, and maintain the elasticity and softness of the skin.

[0012] Further, the compound extraction method of the Abelmoschus moschatus extract and evening primrose root extract is as follows: Step 1: Add Abelmoschus moschatus and evening primrose root into an ethanol solution with an ethanol concentration of 95% at 7-12BV, stir and extract at a temperature of 40±5°C for 3-4h, and centrifuge to obtain an extract; Step 2: Concentrate the extract into an extract, add a fat-soluble solvent to the extract and stir and extract for 1-1.5h, and centrifuge to obtain a supernatant.

[0013] In the technical solution of the present invention, in Step 2, the extract can be concentrated into an extract by using conventional techniques in the art; preferably, the specific conditions for concentration in Step 2 are: rotary evaporation under the conditions of a temperature of 60°C, a pressure of -0.08 to -0.1MPa, and a rotation speed of 60r / min.

[0014] Preferably, the fat-soluble solvent includes at least one of triglyceride (ethylhexanoate), caprylic / capric triglyceride, octyldodecanol, coco-caprylate / caprate, ethylhexyl triazone, cetyl ethylhexanoate, and limnanthes alba seed oil.

[0015] It should be noted that the main function of the fat-soluble solvent adopted in the technical solution of this application is to exist as a solvent, and it has no obvious influence on the skin anti-wrinkle effect of the composition of this application.

[0016] Preferably, the anti-wrinkle composition of the present invention further includes safflower flower extract.

[0017] The safflower flower extract contains rich unsaturated fatty acids, such as linoleic acid, which is an important component of the skin barrier. It can supplement the intercellular lipids of epidermal cells, repair the stratum corneum, enhance the water-shrinking ability, and reduce fine lines caused by dryness. At the same time, it can also promote the differentiation of keratinocytes, accelerate epidermal renewal, and improve skin roughness and texture. In addition, the metabolite of linoleic acid is prostaglandin E1 (PGE1), which inhibits the production of pro-inflammatory PGE2 and blocks the damage of inflammatory factors (such as IL-1β, IL-6) to fibroblasts. It down-regulates the NF-κB signaling pathway, reduces the activation of MMPs (collagen-degrading enzymes), and protects the dermal matrix; at the same time, it contains a small amount of fat-soluble safflower yellow pigment derivatives, which can dilate capillaries, enhance the blood oxygen and nutrient supply in the dermal layer, promote the excretion of metabolic wastes, and improve skin relaxation and wrinkles.

[0018] More preferably, the anti-wrinkle composition of the present invention is obtained by compound extraction of abelmoschus moschatus, evening primrose root, and safflower in a weight ratio of 2-8:3-9:5-20.

[0019] Furthermore, the compound extraction method of the abelmoschus moschatus extract, evening primrose root extract, and safflower flower extract is as follows: Step 1: Add abelmoschus moschatus, evening primrose root, and safflower to an ethanol solution with an ethanol concentration of 95% at 7-12 BV, stir and extract at a temperature of 40±5°C for 3-4 hours, and centrifuge to obtain an extract. Step 2: Concentrate the extract into an extract, add a fat-soluble solvent to the extract, stir and extract for 1-1.5 hours, and centrifuge to obtain a supernatant.

[0020] Preferably, the fat-soluble solvent includes at least one of triglyceride (ethylhexanoate), caprylic / capric triglyceride, octyldodecanol, coco-caprylate / caprate, ethylhexyl triazone, cetyl ethylhexanoate, and limnanthes alba seed oil.

[0021] In the technical solution of the present invention, in step 2, the extract can be concentrated into an extract by using conventional techniques in the art; preferably, the specific conditions for concentration in step 2 are: rotary evaporation at a temperature of 60°C, a pressure of -0.08 to -0.1 MPa, and a rotation speed of 60 r / min.

[0022] In the present invention, BV refers to bed volume, which is abbreviated as BV. Resins are commonly used in chemical production. According to the amount of resin filled in the resin column, a solution is passed through the column. When 1 L of resin is used to pass 1 L of solution, it is called 1 BV. This concept has been extended in the plant extraction industry and is usually used to represent the weight of the extraction solvent added according to the weight of the raw material, which can also be called the multiple. For example, when 1 kg of raw material is extracted with 10 kg of water, it is recorded as 10 BV. In this way, the relationship between the amount of raw medicinal materials input and the amount of solvent can be clarified during the product development and production process.

[0023] The present invention also discloses the use of the above anti-wrinkle composition in preparing skin care products.

[0024] Meanwhile, the present invention discloses a skin care product containing 0.01 - 50 wt% of the above anti-wrinkle composition.

[0025] Beneficial effects Compared with the prior art, the present invention provides an anti-wrinkle extract. By compounding the abelmoschus moschatus extract and the evening primrose root extract, the skin anti-wrinkle effect of the composition can be synergistically improved. Brief description of the drawings

[0026] The present invention will be further described below in conjunction with the drawings and embodiments; Figure 1 It is the β-galactosidase staining map of human fibroblasts in the negative control group of the present invention; Figure 2 It is the β-galactosidase staining map of human fibroblasts in Example 4 (0.1%) of the present invention; Figure 3 It is the chicken embryo map before the use of Example 4 of the present invention; Figure 4 It is the chicken embryo map after the use of Example 4 of the present invention. Detailed description of the invention

[0027] The present invention will be further described below in conjunction with the embodiments, but it does not constitute any limitation to the present invention. Any limited modifications made within the scope of the claims of the present invention are still within the scope of the claims of the present invention.

[0028] In order to elaborate on the technical content of the present invention in detail, the following further explanations are made in conjunction with the embodiments.

[0029] In the following examples and comparative examples, the abelmoschus moschatus was purchased from Bozhou Pocket Doctor Biotechnology Co., Ltd., and the evening primrose root and safflower were both purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd.

[0030] Unless otherwise specified, in the following examples and comparative examples, the parts and % are parts by weight and weight percentage, respectively.

[0031] Example 1 An anti-wrinkle composition is obtained by the following steps: Step 1: Add 5 parts of abelmoschus moschatus and 5 parts of evening primrose root to 10 BV of ethanol solution with an ethanol concentration of 95%, stir and extract at a temperature of 40 ± 5 °C for 4 h, and centrifuge to obtain an extract; Step 2: Rotavapor and concentrate the extract into an extract paste under the conditions of a temperature of 60 °C, a pressure of -0.09 MPa, and a rotation speed of 60 r / min. Add 30 times the weight of the extract paste of triglyceride (ethylhexanoate) to the extract paste, stir and extract for 1 h, and centrifuge to obtain a supernatant.

[0032] Example 2 It is generally the same as Example 1, except that the dosages of the abelmoschus moschatus and evening primrose root are 7 parts and 3 parts, respectively.

[0033] Example 3 It is generally the same as Example 1, except that the dosages of the abelmoschus moschatus and evening primrose root are 3 parts and 9 parts, respectively.

[0034] Example 4 An anti-wrinkle composition is obtained by the following steps: Step 1: Add 5 parts of abelmoschus moschatus, 5 parts of evening primrose root and 10 parts of safflower to 10 BV of ethanol solution with an ethanol concentration of 95%, stir and extract at a temperature of 40 ± 5 °C for 4 h, and centrifuge to obtain an extract; Step 2: Rotavapor and concentrate the extract into an extract paste under the conditions of a temperature of 60 °C, a pressure of -0.09 MPa, and a rotation speed of 60 r / min. Add 30 times the weight of the extract paste of triglyceride (ethylhexanoate) to the extract paste, stir and extract for 1 h, and centrifuge to obtain a supernatant.

[0035] Example 5 It is generally the same as Example 4, except that the dosages of the abelmoschus moschatus, evening primrose root and safflower are 2 parts, 4 parts and 18 parts, respectively.

[0036] Comparative Example 1 An abelmoschus moschatus extract is obtained by the following steps: Step 1: Add 10 parts of abelmoschus moschatus to 10 BV of ethanol solution with an ethanol concentration of 95%, stir and extract at a temperature of 40 ± 5 °C for 4 h, and centrifuge to obtain an extract; Step 2: The extract was rotary evaporated and concentrated into an extract paste at a temperature of 60°C, a pressure of -0.09 MPa, and a rotation speed of 60 r / min. Then, triglyceride (ethylhexanoate) with a weight 30 times that of the extract paste was added thereto, and the mixture was stirred and extracted for 1 h, followed by centrifugation to obtain the supernatant.

[0037] Comparative Example 2 An evening primrose root extract was obtained by the following steps: Step 1: 10 parts of evening primrose roots were added to 10 BV of an ethanol solution with an ethanol concentration of 95%, and the mixture was stirred and extracted at a temperature of 40 ± 5°C for 4 h, followed by centrifugation to obtain the extract; Step 2: The extract was rotary evaporated and concentrated into an extract paste at a temperature of 60°C, a pressure of -0.09 MPa, and a rotation speed of 60 r / min. Then, triglyceride (ethylhexanoate) with a weight 30 times that of the extract paste was added thereto, and the mixture was stirred and extracted for 1 h, followed by centrifugation to obtain the supernatant.

[0038] Comparative Example 3 A safflower flower extract was obtained by the following steps: Step 1: 10 parts of safflower were added to 10 BV of an ethanol solution with an ethanol concentration of 95%, and the mixture was stirred and extracted at a temperature of 40 ± 5°C for 4 h, followed by centrifugation to obtain the extract; Step 2: The extract was rotary evaporated and concentrated into an extract paste at a temperature of 60°C, a pressure of -0.09 MPa, and a rotation speed of 60 r / min. Then, triglyceride (ethylhexanoate) with a weight 30 times that of the extract paste was added thereto, and the mixture was stirred and extracted for 1 h, followed by centrifugation to obtain the supernatant.

[0039] Comparative Example 4 An anti-wrinkle composition was obtained by the following steps: Step 1: 10 parts of Abelmoschus moschatus and 10 parts of safflower were added to 10 BV of an ethanol solution with an ethanol concentration of 95%, and the mixture was stirred and extracted at a temperature of 40 ± 5°C for 4 h, followed by centrifugation to obtain the extract; Step 2: The extract was rotary evaporated and concentrated into an extract paste at a temperature of 60°C, a pressure of -0.09 MPa, and a rotation speed of 60 r / min. Then, triglyceride (ethylhexanoate) with a weight 30 times that of the extract paste was added thereto, and the mixture was stirred and extracted for 1 h, followed by centrifugation to obtain the supernatant.

[0040] Comparative Example 5 An anti-wrinkle composition was obtained by the following steps: Step 1: 10 parts of evening primrose roots and 10 parts of safflower were added to 10 BV of an ethanol solution with an ethanol concentration of 95%, and the mixture was stirred and extracted at a temperature of 40 ± 5°C for 4 h, followed by centrifugation to obtain the extract; Step 2: The extract was rotary evaporated and concentrated into an extract under the conditions of a temperature of 60°C, a pressure of -0.09 MPa, and a rotation speed of 60 r / min. Three times the weight of the extract of triglyceride (ethylhexanoate) was added to the extract and stirred for 1 h, and then centrifuged to obtain the supernatant.

[0041] Efficacy test I. Elastase inhibition rate 1 Test purpose and principle The anti-wrinkle efficacy is mainly characterized by evaluating the inhibition rate of the test sample on elastase. Elastase is mainly synthesized and secreted by fibroblasts, and can degrade elastin in the skin, leading to skin aging. The experimental principle of inhibiting elastase is that porcine pancreatic elastase undergoes a catalytic reaction with the enzyme substrate, and the absorbance changes after adding the active substance. The inhibition rate of the elastase inhibitor is reflected by the magnitude of the absorbance change.

[0042] 2 Test indicators Elastase inhibition rate judgment criterion: If the elastase inhibition rate of the sample is higher than that of the negative control, it can be considered that the test sample has a certain anti-wrinkle effect.

[0043] 3 Test materials and methods 3.1 Instrument and equipment: BSA224S analytical balance, RT-6100 microplate reader.

[0044] 3.2 Reagents: Elastase (porcine pancreas), BR; N-succinyl-L-alanyl-L-alanyl-L-alanine, 98%; Epigallocatechin gallate (EGCG), 98%.

[0045] 3.3 Test method (1) Treatment of control and test samples Sample group: Examples 1-5 and Comparative Examples 1-5 diluted to a concentration of 5% with absolute ethanol; Negative control: pure water.

[0046] (2) Test operation steps Set up a sample group, a sample background group, a solvent group, and a solvent background group, with 3 parallels for each group. Add different reagent solutions to the 96-well plate, shake gently, incubate at 25°C for 15 min, then place it in the microplate reader and measure the absorbance at 410 nm.

[0047] (3) Calculation formula ; Where: A—the absorbance of the reaction solution without the sample and containing the enzyme; B—the absorbance of the reaction solution without the sample and without the enzyme; C—the absorbance of the reaction solution containing the sample and the enzyme; D—the absorbance of the reaction solution containing the sample and without the enzyme.

[0048] The test was carried out according to the above method, and the results are shown in Table 1; Table 1 Results of elastase inhibition rate of Examples 1 - 5 and Comparative Examples 1 - 4 Group Elastase inhibition rate % Example 1 40.22 Example 2 35.48 Example 3 38.67 Example 4 49.32 Example 5 45.77 Comparative Example 1 11.13 Comparative Example 2 29.92 Comparative Example 3 15.79 Comparative Example 4 14.95 Comparative Example 5 19.75 Negative control 1.92

[0049] It can be seen from the results in Table 1 that: It can be known from the data of Examples 1 - 3 that the compound of the okra extract and the evening primrose root extract obtained by the compound extraction of okra and evening primrose root in the present invention can have a relatively high elastase inhibition rate; and according to the data comparison among Examples 1 - 3, both okra and evening primrose root are important components, rather than one of them being the main component and the other being the auxiliary role; the dosage ratio of okra and evening primrose root will also have a certain impact on the elastase inhibition rate of the composition.

[0050] It can be known from the data comparison between Example 4 and Example 1 that the anti-wrinkle composition obtained by adding safflower for compound extraction on the basis of the compound of okra and evening primrose root has a more significant elastase inhibition rate.

[0051] At the same time, according to the data comparison among Example 1, Example 4 and Comparative Examples 1 - 3, it can be seen that the present application has significantly improved the elastase inhibition rate through the compound of okra extract and evening primrose root extract, indicating that the okra extract and evening primrose root extract have a synergistic effect on improving the elastase inhibition rate of the composition; At the same time, Example 4 of the present application has a more obvious improvement in the elastase inhibition rate by adding safflower extract on the basis of okra extract and evening primrose root extract. It is speculated that one of the following reasons may be: (1) There is an obvious compound synergistic effect between okra extract and safflower extract; (2) There is an obvious compound synergistic effect between evening primrose root extract and safflower extract; (3) As a synergistic component, safflower extract can further enhance the synergistic effect of okra extract and evening primrose root extract.

[0052] In order to verify the above reasons, the present application verified whether there is a synergistic effect between okra extract and safflower extract, and between evening primrose root extract and safflower extract respectively through Comparative Example 4 and Comparative Example 5; It can be known from the data comparison of Example 4 and Comparative Examples 1 - 5 in Table 1 that: (1) There is only a simple additive effect between the abelmoschus moschatus extract and the carthamus tinctorius flower extract, or the synergistic effect between the two is not obvious; (2) There is only a simple additive effect between the evening primrose root extract and the carthamus tinctorius flower extract, or the synergistic effect between the two is not obvious; Thus, it can be seen that Example 4 of this application has produced a further synergistic effect by adding the carthamus tinctorius flower extract on the basis of the abelmoschus moschatus extract and the evening primrose root extract. The carthamus tinctorius flower extract, as a significant synergistic component, further enhances the synergistic effect of the abelmoschus moschatus extract and the evening primrose root extract.

[0053] II. β-galactosidase staining experiment of human fibroblasts 1 Test purpose and principle Cellular senescence is a persistent state of cell cycle arrest that accumulates in aging organisms, and wrinkles are an external manifestation of cellular senescence. Human fibroblasts are induced with hydrogen peroxide (H2O2), and with X-Gal as a substrate, senescent cells will produce a dark blue product under the catalysis of β-galactosidase.

[0054] Human fibroblasts can be used as a cell model to study the anti-wrinkle efficacy of cosmetics. By comparing the β-galactosidase staining degree of cells in the negative control group with that in the sample group, the efficacy intensity of the sample can be evaluated.

[0055] 2 Test indicators Judgment criterion for the β-galactosidase staining degree of human fibroblasts: The lower the β-galactosidase staining degree of human fibroblasts, that is, the higher the efficacy intensity of the sample and the better the anti-wrinkle efficacy.

[0056] 3 Test materials and methods 3.1 Instrument and equipment: MF52-N inverted fluorescence microscope.

[0057] 3.2 Cells used in the experiment: Human fibroblasts.

[0058] 3.3 Test method (1) Treatment of test samples (β-galactosidase staining test of human fibroblasts)

[0059] Sample group: Basic culture medium containing H2O2 and 0.1% sample.

[0060] Negative control: Basic culture medium containing H2O2.

[0061] (2) Test operation steps Human fibroblast β-galactosidase staining assay: After culturing human fibroblasts in a 6-well plate for 24 h, add β-galactosidase fixing solution and fix at room temperature for 15 min. Then add β-galactosidase staining working solution to each well and incubate overnight at 37 °C. Observe the blue-stained cells under an ordinary optical microscope.

[0062] (3)Data analysis Use analysis software to perform quantitative analysis on the stained pictures.

[0063] Perform the test on Example 4 according to the above test method, and the results are shown in Table 2 and Figure 1-2 as follows, where the improvement rate % = (negative control staining degree - sample staining degree) / negative control staining degree * 100%; Figure 1 is the β-galactosidase staining picture of human fibroblasts in the negative control group, Figure 2 is the β-galactosidase staining picture of human fibroblasts of 0.1% Example 4.

[0064] Table 2 Results of the β-galactosidase staining experiment of human fibroblasts in Example 4 Group Degree of staining Improvement rate % Example 4 6.87 66.22 Negative control 20.33 /

[0065] It can be seen from the results in Table 2 that: Example 4 of this application has a significant skin anti-wrinkle effect.

[0066] III. Irritation experiment Test method: Chicken embryo chorioallantoic membrane test 1. Test purpose and principle The chicken embryo chorioallantoic membrane test is an in vitro evaluation method for eye irritation that has been used earlier. The chorioallantoic membrane (CAM) is a respiratory membrane that surrounds the chicken embryo. This test takes advantage of the characteristics of the intact, clear, and transparent vascular system of the chorioallantoic membrane in the middle stage of the hatched chicken embryo. A certain amount of the test substance is directly contacted with the chorioallantoic membrane of the chicken embryo, and after acting for a period of time, the changes in the toxicity effect indicators of the chorioallantoic membrane (such as bleeding, coagulation, and vascular lysis) are observed. These indicators reflect the changes in the morphological structure, color, and permeability of blood vessels and vascular networks, as well as the phenomena of protein denaturation in the chorioallantoic membrane and its damaged degree. Then a score is combined to evaluate the eye irritation of the test substance.

[0067] The purpose of this test is to test the ability of the test substance to cause toxicity changes in the chicken embryo chorioallantoic membrane and evaluate the elements and processes of the potential eye irritation of the substance to be evaluated.

[0068] 2. Treatment of test groups and reagents Sample group: Dilute Example 4 into a 2% oil solution.

[0069] Negative control: 0.9% normal saline.

[0070] Positive control: 1% sodium dodecyl sulfate (SDS) solution.

[0071] 3. Test procedure For each group in this test, 6 chicken embryos were selected. The situation of the chorioallantoic membrane was recorded with a photographing device. A polytetrafluoroethylene resin ring was placed on the chorioallantoic membrane of the chicken embryo and photographed. The test sample was added into the polytetrafluoroethylene resin ring, the time of adding the sample was recorded, and the air chamber was covered with a moistened plastic wrap. The chicken embryo was transferred to a constant temperature and humidity incubator for cultivation, and the degree of change in each toxic effect was observed.

[0072] 4. Result calculation The endpoint evaluation method was used for the test, and the endpoint evaluation (ES) was calculated, with the result reserved to two decimal places; the score of each chicken embryo was the sum of the degrees of bleeding, blood coagulation, and blood vessel lysis observed in each chicken embryo; ES was the average value of the mathematical sum obtained from 6 chicken embryos.

[0073] The average score of ES was calculated according to the following formula: 。

[0074] 5. Result judgment criteria ES ≤ 4, non-irritating; 4 < ES ≤ 12, slightly irritating; 12 < ES < 16, moderately irritating; ES ≥ 16, strongly irritating / corrosive.

[0075] Test results According to the above test method, Example 4 was sent for testing, ES = 2.00, and the result was judged as non-irritating; The results of the chicken embryos before and after using Example 4 are as Figure 3 、 Figure 4 shown, proving that the technical solution of the present invention is mild and non-irritating.

[0076] Application example An anti-wrinkle product, the formula is shown in Table 3: Table 3 Formula table of the anti-wrinkle product

[0077] An anti-wrinkle product, the formula refers to Table 3, and the specific preparation method is as follows: Step 1: Phase A was preheated to 80 - 85 °C for dissolution, stirred and mixed evenly, and reserved; Step 2: The raw materials in Phase B were added into the main pot in sequence and stirred evenly; Step 3: Add Phase A and Phase C, stir evenly, and that's it.

[0078] The embodiments presented herein are only the implementations selected according to the combinations of all possible embodiments. The appended claims should not be limited by the embodiments that illustrate the invention. Some numerical ranges used in the claims include sub-ranges within them, and the variations in these ranges should also be covered by the appended claims.

Claims

1. An anti-wrinkle composition, characterized in that, It includes abelmoschus moschatus extract and evening primrose root extract, and the abelmoschus moschatus extract and evening primrose root extract are obtained by compound extraction of abelmoschus moschatus and evening primrose root in a weight ratio of 3-7:3-9.

2. The anti-wrinkle composition according to claim 1, characterized in that, It also includes safflower flower extract.

3. The anti-wrinkle composition according to claim 1, characterized in that, The compound extraction method of the abelmoschus moschatus extract and evening primrose root extract is as follows: Step 1: Add abelmoschus moschatus and evening primrose root into an ethanol solution with an ethanol concentration of 95% at 7-12 BV, stir and extract at a temperature of 40±5°C for 3-4 h, and centrifuge to obtain an extract; Step 2: Concentrate the extract into an extract, add a fat-soluble solvent to the extract and stir and extract for 1-1.5 h, and centrifuge to obtain a supernatant; the weight ratio of the extract to the fat-soluble solvent is 7-12:150-400.

4. The anti-wrinkle composition according to claim 3, characterized in that, The fat-soluble solvent includes at least one of triglyceride (ethylhexanoate), caprylic / capric triglyceride, octyldodecanol, coco-caprylate / caprate, ethylhexyl triazone, cetyl ethylhexanoate, and limnanthes alba seed oil.

5. Use of the anti-wrinkle composition as described in any one of claims 1-4 for preparing skin care products.

6. A skin care product, characterized in that, It contains 0.01-50 wt% of the anti-wrinkle composition as described in any one of claims 1-4.

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