Growth regulator for improving drought resistance of wheat in booting stage, preparation method and application

Abstract

The invention relates to a growth regulator for improving the drought resistance of wheat in the booting stage, a preparation method and application, and the growth regulator takes a composition containing spermidine, 2, 4-epibrassinolide and monopotassium phosphate as an active ingredient. According to the growth regulator for improving the drought resistance of wheat in the booting stage, the drought resistance of wheat in the booting stage can be improved, and the drought resistance of wheat in the later growth stage can be greatly improved; moreover, spermidine, 2, 4-epibrassinolide and monopotassium phosphate are specifically combined and proportioned and are applied to improve the drought resistance of wheat at the booting stage for the first time, the three active ingredients have a synergistic effect, the comprehensive drought resistance of wheat can be remarkably improved, so that the grain yield is stabilized, and meanwhile, the composition has the characteristics of safety, high efficiency and environmental protection, and is worthy of popularization and application. The preparation method is simple and easy to implement, and is convenient to popularize and apply.

Description

Technical Field

[0001] This application relates to the technical field of wheat cultivation, and in particular, to a composition, a growth regulator, a preparation method and an application for improving the drought resistance of wheat at the booting stage. Background Art

[0002] Wheat is the most widely planted crop in the world, feeding more than one-third of the global population. At the same time, wheat is also one of the main food crops in China. In 2024, the planting area of wheat in China was 2.36×10 7 hm 2 , and the yield reached 1.40×10 11 kg, accounting for 19.8% of the total national grain output. In recent years, with the frequent occurrence of extreme weather, drought events will occur more frequently and severely. 45% of the agricultural land globally has been affected by continuous drought stress. It is predicted that by the end of this century, due to climate change, 60% of the current wheat planting areas will face serious water shortage problems. At the same time, the water resources in China are highly unevenly distributed in terms of time and space, with a small per capita water resource possession, and are more vulnerable to the dual constraints of climate drought and water resource shortage. According to statistics, in 2021, the affected area of drought disasters in China was 3.426 million hm 2 , and the drought-affected area of wheat in 8 provinces such as Henan, Shandong and Hebei accounted for about 40% of their winter wheat planting area. Therefore, there is an urgent need for drought-resistant cultivation or breeding measures to stabilize food production under climate change.

[0003] Drought stress occurs throughout the whole growth period of wheat, but the frequency of drought is the highest at the booting stage. During the booting stage, the tetrad stage and microspore formation stage of florets are the most sensitive to the external environment. Encountering drought stress will lead to a higher degradation rate and abortion rate of wheat florets, significantly reducing the number of grains per ear.

[0004] In the existing technology, wheat drought resistance is usually treated by seed treatment, drought-resistant variety breeding and spraying drought-resistant and strong growth regulators. Among them, seed treatment mainly improves the drought resistance of wheat by soaking seeds with calcium chloride, chlormequat chloride and microbial agents, or by using polyethylene glycol and other progressive drought stress treatments to eliminate the sensitivity of wheat seeds to water and stimulate their adaptability to drought stress; however, seed treatment technology seed treatment is mainly aimed at the germination period and seedling stage, and has limited effect on improving the drought resistance in the later period, and some chemical agents may cause pollution to the soil and environment, and are not suitable for long-term use. Variety breeding mainly cultivates drought-resistant varieties with well-developed root systems, weak transpiration, drought resistance and water conservation, and can significantly increase yields through hybrid breeding or genetic engineering; however, the cycle required for breeding new varieties is too long, the success rate is low, and genetic engineering and hybrid breeding technologies require a lot of money. The main method of spraying drought-resistant and strong growth regulators is to spray paclobutrazol and seaweed extracts to promote root development, salicylic acid and betaine to enhance antioxidant capacity to maintain water balance and protect enzymes and membrane structures, thereby accelerating the growth of crop roots and leaves, improving the drought resistance of crops in arid environments, enhancing the metabolism and photosynthetic capacity of crops, and improving the yield and quality of crops. However, the drought-resistant and strong growth regulators currently sprayed mainly rely on a few types such as abscisic acid AB A and paclobutrazol, with a single active ingredient and some containing chemically synthesized ingredients. Long-term use may cause soil and water pollution, affecting the ecological environment and the safety of agricultural products.

[0005] Therefore, it is necessary to improve one or more problems existing in the above-mentioned related technical solutions.

[0006] It should be noted that the information disclosed in the above background technology section is only used to enhance the understanding of the background of the present disclosure, and therefore may include information that does not constitute prior art known to ordinary technicians in the field. Summary of the Invention

[0007] The purpose of the embodiments of the present disclosure is to provide a growth regulator, preparation method and application for improving the drought resistance of wheat during the heading period, thereby overcoming one or more problems caused by the limitations and defects of related technologies to at least a certain extent.

[0008] In a first aspect, the present application provides a composition for improving the drought resistance of wheat during the heading period, wherein the composition comprises, by weight, 1-5 parts of spermidine, 0.0001-0.004 parts of 2,4-epibrassinolide, and 11-15 parts of potassium dihydrogen phosphate.

[0009] In a possible embodiment, the components are composed of 2-4 parts of spermidine, 0.0002-0.002 parts of 2,4-epibrassinolide, and 12-14 parts of potassium dihydrogen phosphate in parts by mass.

[0010] Second aspect, the present application provides a growth regulator for improving the drought resistance of wheat at the booting stage, and its active ingredient is the composition described in the first aspect.

[0011] In a possible implementation manner, it further contains a pharmaceutically acceptable surfactant and an adhesive.

[0012] In a possible implementation manner, the surfactant and the adhesive are Tween-20.

[0013] In a possible implementation manner, the ratio of spermidine to the surfactant and the adhesive is 50 - 250 mg: 0.1 ml.

[0014] In a possible implementation manner, the ratio of spermidine to the surfactant and the adhesive is 100 - 200 mg: 0.1 ml.

[0015] Third aspect, the present application provides a preparation method of a growth regulator for improving the drought resistance of wheat at the booting stage, which is used to prepare the growth regulator for improving the drought resistance of wheat at the booting stage described in the second aspect, and includes: dissolving and mixing spermidine, 2,4-epibrassinolide and potassium dihydrogen phosphate with clear water containing Tween-20 to obtain the product.

[0016] Fourth aspect, the application of the composition described in the first aspect of the present application, or the growth regulator described in the second aspect, in improving the drought resistance of wheat at the booting stage.

[0017] The technical solution provided by the present application may include the following beneficial effects:

[0018] The composition and the growth regulator for improving the drought resistance of wheat at the booting stage provided by the present application can enhance the drought resistance of wheat at the booting stage and significantly improve the drought resistance of wheat in the later growth stage; moreover, by carrying out a specific combined ratio of spermidine, 2,4-epibrassinolide and potassium dihydrogen phosphate and applying it to improving the drought resistance of wheat for the first time, the three active ingredients act synergistically, can significantly improve the comprehensive drought resistance of wheat, thereby stabilizing the grain yield; at the same time, it has the characteristics of safety, high efficiency and environmental protection, can be used for a long time, and the preparation method is simple and easy to implement, which is convenient for popularization and application.

[0019] It should be understood that the above general description and the following detailed description are only exemplary and explanatory, and cannot limit the present disclosure. Description of the Drawings

[0020] The accompanying drawings here are incorporated into the specification and form a part of this specification, showing embodiments consistent with the present disclosure, and are used together with the specification to explain the principles of the present disclosure. Obviously, the accompanying drawings in the following description are only some embodiments of the present disclosure. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on these drawings.

[0021] Figure 1 It is a result graph of the growth regulator for improving the drought resistance of wheat at the booting stage in Example 1 of this application and its individual active ingredients in improving the 1000-grain weight, number of grains per spike, number of spikes, and yield.

[0022] Figure 2 It is a result graph of the growth regulator for improving the drought resistance of wheat at the booting stage in Example 1 of this application and its individual active ingredients in improving the chlorophyll content and net photosynthetic rate.

[0023] Figure 3 It is a result graph of the growth regulator for improving the drought resistance of wheat at the booting stage in Example 2 of this application and its individual active ingredients in improving the 1000-grain weight, number of grains per spike, number of spikes, and yield.

[0024] Figure 4 It is a result graph of the growth regulator for improving the drought resistance of wheat at the booting stage in Example 2 of this application and its individual active ingredients in improving the chlorophyll content and net photosynthetic rate.

[0025] Figure 5 It is a result graph of the growth regulator for improving the drought resistance of wheat at the booting stage in Example 3 of this application and its individual active ingredients in improving the 1000-grain weight, number of grains per spike, number of spikes, and yield.

[0026] Figure 6 It is a result graph of the growth regulator for improving the drought resistance of wheat at the booting stage in Example 3 of this application and its individual active ingredients in improving the chlorophyll content and net photosynthetic rate.

[0027] Figure 7 It is a result graph of the growth regulator for improving the drought resistance of wheat at the booting stage in Example 4 of this application and its individual active ingredients in improving the 1000-grain weight, number of grains per spike, number of spikes, and yield.

[0028] Figure 8 It is a result graph of the growth regulator for improving the drought resistance of wheat at the booting stage in Example 4 of this application and its individual active ingredients in improving the chlorophyll content and net photosynthetic rate.

[0029] Figure 9 It is a result graph of the growth regulator for improving the drought resistance of wheat at the booting stage in Example 5 of this application and its individual active ingredients in improving the 1000-grain weight, number of grains per spike, number of spikes, and yield.

[0030] Figure 10 This is the result graph of the growth regulator for improving the drought resistance of wheat at the booting stage in Example 5 of this application and the ability of its individual active ingredients to increase chlorophyll content and net photosynthetic rate;

[0031] Figure 11 This is the result graph of the growth regulator for improving the drought resistance of wheat at the booting stage in Example 6 of this application and the ability of its individual active ingredients to increase chlorophyll content and net photosynthetic rate;

[0032] Figure 12 This is the result graph of the growth regulator for improving the drought resistance of wheat at the booting stage in Example 6 of this application and the ability of its individual active ingredients to increase chlorophyll content and net photosynthetic rate;

[0033] Figure 13 This is the result graph of the growth regulator for improving the drought resistance of wheat at the booting stage in Example 7 of this application and the ability of its individual active ingredients to increase chlorophyll content and net photosynthetic rate;

[0034] Figure 14 This is the result graph of the growth regulator for improving the drought resistance of wheat at the booting stage in Example 7 of this application and the ability of its individual active ingredients to increase chlorophyll content and net photosynthetic rate;

[0035] Figure 15 This is the result graph of the growth regulator for improving the drought resistance of wheat at the booting stage in Example 8 of this application and the ability of its pairwise compounded active ingredients to increase chlorophyll content and net photosynthetic rate;

[0036] Figure 16 This is the result graph of the growth regulator for improving the drought resistance of wheat at the booting stage in Example 8 of this application and the ability of its pairwise compounded active ingredients to increase chlorophyll content and net photosynthetic rate;

[0037] Figure 17 This is the result graph of the growth regulator for improving the drought resistance of wheat at the booting stage in Example 9 of this application and the ability of its pairwise compounded active ingredients to increase chlorophyll content and net photosynthetic rate;

[0038] Figure 18 This is the result graph of the growth regulator for improving the drought resistance of wheat at the booting stage in Example 9 of this application and the ability of its pairwise compounded active ingredients to increase chlorophyll content and net photosynthetic rate;

[0039] Figure 19 This is the result graph of the growth regulator for improving the drought resistance of wheat at the booting stage in Example 10 of this application and the ability of its pairwise compounded active ingredients to increase chlorophyll content and net photosynthetic rate;

[0040] Figure 20This is a result graph showing the ability of the growth regulator for improving the drought resistance of wheat at the booting stage in Example 10 of this application and its binary compound active ingredients to increase chlorophyll content and net photosynthetic rate. Detailed implementation mode

[0041] Example embodiments will now be described more fully with reference to the accompanying drawings. However, the example embodiments can be implemented in various forms and should not be construed as limited to the examples set forth herein; rather, these embodiments are provided so that this disclosure will be more complete and comprehensive, and will fully convey the concept of the example embodiments to those skilled in the art. The described features, structures, or characteristics can be combined in any suitable manner in one or more embodiments. For those not specified in the examples regarding specific techniques or conditions, they shall be carried out according to the techniques or conditions described in the literature in this field or according to the product specifications. For the reagents or instruments not indicating the manufacturer, they are all conventional products that can be obtained through market purchase.

[0042] This application provides a composition for improving the drought resistance of wheat at the booting stage. Calculated by mass parts, its components are: spermidine 1 - 5 parts, 2,4-epibrassinolide 0.0001 - 0.004 parts, potassium dihydrogen phosphate 11 - 15 parts.

[0043] It should be noted that preferably, the composition for improving the drought resistance of wheat at the booting stage, calculated by mass parts, its components are: spermidine 2 - 4 parts, 2,4-epibrassinolide 0.0002 - 0.002 parts, potassium dihydrogen phosphate 12 - 14 parts.

[0044] This application also provides a growth regulator with the above-mentioned composition for improving the drought resistance of wheat at the booting stage as the active ingredient.

[0045] In a possible implementation mode, the growth regulator contains a surfactant and an adhesive acceptable in pesticide science.

[0046] It can be understood that the surfactant and adhesive acceptable in pesticide science are used to improve the wetting and penetration ability of pesticides, enabling pesticides to penetrate quickly, reduce the surface tension to make pesticides spread evenly, and increase the adhesion to extend the retention time of pesticides. Commonly used surfactants and adhesives include: anionic surfactants, non-ionic surfactants, cationic surfactants, and amphoteric surfactants.

[0047] Furthermore, the surfactant and adhesive acceptable in pesticide science in this application are Tween-20.

[0048] It is understandable that Tween-20 is a non-ionic surfactant and an atypical adhesive. While it can enhance the emulsification, dispersion, wetting, and solubilization effects of pesticides, the molecular structure of Tween-20 contains hydrophilic polyoxyethylene chains and hydrophobic fatty acid groups, which enables it to reduce the surface tension of the liquid, allowing the pesticide droplets to spread better on the target surface, thereby indirectly enhancing the adhesion of pesticides to the target.

[0049] In a possible embodiment, the ratio of spermidine to the surfactant and the adhesive is 50 - 250 mg: 0.1 ml.

[0050] Furthermore, the ratio of spermidine to the surfactant and the adhesive is 100 - 200 mg: 0.1 ml.

[0051] It is understandable that in this application, when the range of spermidine is 50 - 250 mg and 100 - 200 mg, the addition amount of Tween-20 is 0.1 ml in both cases.

[0052] Furthermore, this application also provides a preparation method of a growth regulator for enhancing the drought resistance of wheat at the booting stage. Dissolve spermidine, brassinolide, and potassium dihydrogen phosphate in clear water containing Tween-20 and mix them evenly to obtain the product.

[0053] It is understandable that spermidine, brassinolide, and potassium dihydrogen phosphate in the preparation method are the above-mentioned composition for enhancing the drought resistance of wheat at the booting stage, and the addition amount of Tween-20 is also added in the proportion in the above-mentioned growth regulator.

[0054] The composition for enhancing the drought resistance of wheat at the booting stage and the growth regulator for enhancing the drought resistance of wheat at the booting stage in this application are used for spraying before the booting stage of wheat, so as to enhance the comprehensive drought resistance of wheat and thus stabilize the grain yield.

[0055] Example 1

[0056] Comparison of the drought resistance growth regulator of this application and its individual active ingredients in enhancing the drought resistance of wheat and stabilizing the grain yield.

[0057] The active ingredients of the growth regulator in this example are counted by mass parts as follows: 2 parts of spermidine, 0.0002 parts of 2,4-epibrassinolide, and 12 parts of potassium dihydrogen phosphate.

[0058] Take an appropriate amount of spermidine (purchased from Shanghai Merck Chemical Technology Co., Ltd., China) and 0.1 ml of Tween-20, and prepare a solution with a final concentration of spermidine of 100 mg / L (Spd2).

[0059] Take an appropriate amount of 2,4-epibrassinolide and 0.1 ml of Tween-20 to prepare a solution with a final concentration of 0.01 mg / L (EBR2) of 2,4-epibrassinolide.

[0060] Take an appropriate amount of potassium dihydrogen phosphate and 0.1 ml of Tween-20 to prepare a solution with a final concentration of 600 mg / L (K2) of potassium dihydrogen phosphate.

[0061] Take an appropriate amount of spermidine, 2,4-epibrassinolide, potassium dihydrogen phosphate and 0.1 ml of Tween-20 to prepare a solution with final concentrations of 100 mg / L, 0.01 mg / L, 600 mg / L (Spd2+EBR2+K2).

[0062] Select the winter wheat variety Weilong 169 as the material at Caoxinzhuang Farm, Yangling Demonstration Zone, Shaanxi Province (34°18′N, 108°06′E). Under the condition of drought stress (DS) (soil relative humidity 50-55%) during the booting stage of wheat growth, spray spermidine (Spd), 2,4-epibrassinolide (EBR), and potassium dihydrogen phosphate (K), with spraying clear water as the control, that is:

[0063] Drought stress + spraying clear water (DS);

[0064] Drought stress + spraying 100 mg / L spermidine (Spd2);

[0065] Drought stress + spraying 0.01 mg / L 2,4-epibrassinolide (EBR2);

[0066] Drought stress + spraying 600 mg / L potassium dihydrogen phosphate (K2);

[0067] Drought stress + spraying a solution of 100 mg / L spermidine + 0.01 mg / L 2,4-epibrassinolide + 600 mg / L potassium dihydrogen phosphate (Spd2+EBR2+K2).

[0068] The experiment adopted a split-plot experimental design, and the area of each plot was 6 m 2 (2 m × 3 m). Starting 5 days before the booting stage, continuously carry out the above spraying for 2 days at 16:00-18:00 in the afternoon on sunny days, so that the leaves are completely wetted but the liquid does not flow down, that is, the spraying amount is 75 ml / m 2 .

[0069] The experimental results show that the drought-resistant growth regulator and its active ingredients in this example can improve the drought resistance of wheat and stabilize the grain yield. The results of improving the drought resistance of wheat are as Figure 1-2As shown in the results, drought-resistant growth regulators significantly increased wheat 1000-grain weight, number of grains per spike, yield, chlorophyll content, and net photosynthetic rate compared to single application of spermidine, 2,4-epibrassinolide, and potassium dihydrogen phosphate under drought conditions. The highest yield of wheat sprayed with drought-resistant growth regulators reached 7.64t / hm2. 2 Compared with drought, the application of spermidine, 2,4-epibrassinolide and potassium dihydrogen phosphate significantly increased the

[0070] Example 2

[0071] Comparison of the drought-resistant growth regulator of the present application and its single active ingredient in improving the drought resistance of wheat and stabilizing grain yield.

[0072] The active ingredients of the growth regulator of this embodiment are calculated by weight as follows: 3 parts of spermidine, 0.001 parts of 2,4-epibrassinolide, and 13 parts of potassium dihydrogen phosphate.

[0073] Take an appropriate amount of spermidine and 0.1 ml of Tween-20 to prepare a solution with a final spermidine concentration of 150 mg / L (Spd3).

[0074] Take an appropriate amount of 2,4-epibrassinolide and 0.1 ml of Tween-20 to prepare a solution with a final concentration of 2,4-epibrassinolide of 0.05 mg / L (EBR3).

[0075] Take an appropriate amount of potassium dihydrogen phosphate and 0.1 ml of Tween-20 to prepare a potassium dihydrogen phosphate solution with a final concentration of 650 mg / L (K3).

[0076] Take appropriate amounts of spermidine, 2,4-epibrassinolide, potassium dihydrogen phosphate and Tween-20 to prepare solutions with final concentrations of 150 mg / L, 0.05 mg / L and 650 mg / L (Spd3+EBR3+K3).

[0077] Winter wheat variety Weilong 169 was used as the material. At Caoxinzhuang Farm in Yangling Demonstration Zone, Shaanxi Province (34°18′N, 108°06′E), drought stress (DS) (relative soil moisture 50-55%) was applied at the heading stage of wheat growth. Spermidine (Spd), 2,4-epibrassinolide (EBR), and potassium dihydrogen phosphate (K) were sprayed. Water was sprayed as a control.

[0078] drought stress + spraying with water (DS);

[0079] Drought stress + spraying 150 mg / L spermidine (Spd3);

[0080] Drought stress + spraying 0.05 mg / L 2,4-epibrassinolide (EBR3);

[0081] Drought stress + spraying 650 mg / L potassium dihydrogen phosphate (K3); solution of drought stress + spraying 150 mg / L spermidine + 0.05 mg / L 2,4-epibrassinolide + 650 mg / L potassium dihydrogen phosphate (Spd3 + EBR 3 + K3).

[0082] The experiment adopted a split-plot experimental design, and the area of each plot was 6 m 2 (2 m × 3 m). Starting from 5 days before the booting stage, the above spraying was continuously carried out for 2 days at 16:00 - 18:00 in the afternoon on sunny days, so that the leaves were completely wetted but the liquid did not flow down, that is, the spraying amount was 75 ml / m 2 .

[0083] The experimental results show that both the drought-resistant growth regulator and its active ingredients in this example can improve the drought resistance of wheat and stabilize the grain yield. The results of improving the drought resistance of wheat are as Figure 3-4 shown. Compared with the single application of spermidine, 2,4-epibrassinolide, and potassium dihydrogen phosphate under drought, the drought-resistant growth regulator significantly increased the 1000-grain weight, grain number per spike, yield, chlorophyll content, and net photosynthetic rate of wheat. The highest yield under drought after spraying the drought-resistant growth regulator reached 8.00 t / hm 2 , which was significantly increased by 8.48% - 13.21% compared with the single application of spermidine, 2,4-epibrassinolide, and potassium dihydrogen phosphate under drought.

[0084] Example 3

[0085] Comparison of the drought-resistant growth regulator of this application and its individual active ingredients in improving the drought resistance of wheat and stabilizing the grain yield.

[0086] The active ingredients of the growth regulator in this example are calculated by mass fraction as follows: 4 parts of spermidine, 0.002 parts of 2,4-epibrassinolide, and 14 parts of potassium dihydrogen phosphate.

[0087] Take an appropriate amount of spermidine and 0.1 ml of Tween-20 to prepare a solution with a final concentration of 200 mg / L of spermidine (Spd4).

[0088] Take an appropriate amount of 2,4-epibrassinolide and 0.1 ml of Tween-20 to prepare a solution with a final concentration of 0.1 mg / L of 2,4-epibrassinolide (EBR4).

[0089] Take an appropriate amount of potassium dihydrogen phosphate and 0.1 ml of Tween-20 to prepare a solution with a final concentration of 700 mg / L of potassium dihydrogen phosphate (K4).

[0090] Take an appropriate amount of spermidine, 2,4-epibrassinolide, potassium dihydrogen phosphate, and Tween-20 to prepare a solution with final concentrations of 200 mg / L, 0.1 mg / L, and 700 mg / L (Spd4 + EBR4 + K4).

[0091] The winter wheat variety Weilong 169 was selected as the material at Caoxinzhuang Farm, Yangling Demonstration Area, Shaanxi Province (34°18′N, 108°06′E). During the booting stage of wheat growth, when soil moisture was under drought stress (DS) (soil relative humidity 50 - 55%), spermidine (Spd), 2,4-epibrassinolide (EBR), and potassium dihydrogen phosphate (K) were sprayed, with spraying of clear water as the control, namely:

[0092] Drought stress + spraying of clear water (DS);

[0093] Drought stress + spraying of 200 mg / L spermidine (Spd4);

[0094] Drought stress + spraying of 0.1 mg / L 2,4-epibrassinolide (EBR4);

[0095] Drought stress + spraying of 700 mg / L potassium dihydrogen phosphate (K4);

[0096] Drought stress + spraying of a solution containing 200 mg / L spermidine + 0.1 mg / L 2,4-epibrassinolide + 700 mg / L potassium dihydrogen phosphate (Spd4 + EBR4 + K4).

[0097] The split-plot experiment design was adopted, and the area of each plot was 6 m 2 (2 m × 3 m). Starting 5 days before the booting stage, the above spraying was continuously carried out for 2 days at 16:00 - 18:00 in the afternoon on sunny days, making the leaves completely wet but the liquid not flowing down, that is, the spraying amount was 75 ml / m 2 .

[0098] The experimental results showed that both the drought-resistant growth regulator and its active ingredients in this example could improve the drought resistance of wheat and stabilize the grain yield. The results of improving the drought resistance of wheat were as Figure 5-6 shown. Compared with single application of spermidine, 2,4-epibrassinolide, and potassium dihydrogen phosphate under drought, the drought-resistant growth regulator significantly increased the 1000-grain weight, number of grains per spike, yield, chlorophyll content, and net photosynthetic rate of wheat. The highest yield under drought after spraying the drought-resistant growth regulator reached 7.63 t / hm2, which was significantly increased by 9.39% - 15.22% compared with single application of spermidine, 2,4-epibrassinolide, and potassium dihydrogen phosphate under drought.

[0099] Example 4

[0100] Comparison of the drought-resistant growth regulator of this application and its individual active ingredients in improving the drought resistance of wheat and stabilizing the grain yield.

[0101] The active ingredients of the growth regulator in this example were as follows by mass fraction: 2 parts of spermidine, 0.001 part of 2,4-epibrassinolide, and 14 parts of potassium dihydrogen phosphate.

[0102] Take an appropriate amount of spermidine and 0.1 ml of Tween-20 to prepare a solution with a final concentration of 100 mg / L (Sp d2) of spermidine.

[0103] Take an appropriate amount of 2,4-epibrassinolide and 0.1 ml of Tween-20 to prepare a solution with a final concentration of 0.05 mg / L (EBR3) of 2,4-epibrassinolide.

[0104] Take an appropriate amount of potassium dihydrogen phosphate and 0.1 ml of Tween-20 to prepare a solution with a final concentration of 700 mg / L (K4) of potassium dihydrogen phosphate.

[0105] Take an appropriate amount of spermidine, 2,4-epibrassinolide, potassium dihydrogen phosphate and Tween-20 to prepare a solution with final concentrations of 100 mg / L, 0.05 mg / L, 700 mg / L (Spd2+EBR3+K4).

[0106] Select the winter wheat variety Weilong 169 as the material at Caoxinzhuang Farm, Yangling Demonstration Area, Shaanxi Province (34°18′N, 108°06′E). Under the condition of drought stress (DS) (soil relative humidity 50-55%) during the booting stage of wheat growth, spray spermidine (Spd), 2,4-epibrassinolide (EBR), potassium dihydrogen phosphate (K), with spraying clear water as the control, namely:

[0107] Drought stress + spraying clear water (DS);

[0108] Drought stress + spraying 100 mg / L spermidine (Spd2);

[0109] Drought stress + spraying 0.05 mg / L 2,4-epibrassinolide (EBR3);

[0110] Drought stress + spraying 700 mg / L potassium dihydrogen phosphate (K4);

[0111] Drought stress + spraying a solution of 100 mg / L spermidine + 0.05 mg / L 2,4-epibrassinolide + 700 mg / L potassium dihydrogen phosphate (Spd2+EBR3+K4).

[0112] The experiment adopts a split-plot experimental design, and the area of each plot is 6 m 2 (2 m × 3 m). Starting 5 days before the booting stage, continuously carry out the above spraying for 2 days at 16:00-18:00 in the afternoon on sunny days, so that the leaves are completely wet, but the liquid does not flow down, that is, the spraying amount is 75 ml / m 2 .

[0113] The experimental results show that the drought-resistant growth regulator and its active ingredients in this example can improve the drought resistance of wheat and stabilize the grain yield. The results of improving the drought resistance of wheat are asFigure 7-8 As shown, compared with spermidine, 2,4-epibrassinolide, and potassium dihydrogen phosphate applied alone under drought conditions, the drought-resistant growth regulator increased the thousand-grain weight, number of grains per spike, yield, chlorophyll content, and net photosynthetic rate of wheat. The highest yield under drought conditions reached 7.66 t / hm 2 , which was significantly increased by 6.00% - 15.63% compared with spermidine, 2,4-epibrassinolide, and potassium dihydrogen phosphate applied alone under drought conditions.

[0114] Example 5

[0115] Comparison of the drought-resistant growth regulator of this application with its individual active ingredients in improving the drought resistance of wheat and stabilizing grain yield.

[0116] The active ingredients of the growth regulator in this example were as follows by mass fraction: 4 parts of spermidine, 0.001 part of 2,4-epibrassinolide, and 12 parts of potassium dihydrogen phosphate.

[0117] Take an appropriate amount of spermidine and 0.1 ml of Tween-20 to prepare a solution with a final concentration of 200 mg / L (Spd4) of spermidine.

[0118] Take an appropriate amount of 2,4-epibrassinolide and 0.1 ml of Tween-20 to prepare a solution with a final concentration of 0.05 mg / L (EBR3) of 2,4-epibrassinolide.

[0119] Take an appropriate amount of potassium dihydrogen phosphate and 0.1 ml of Tween-20 to prepare a solution with a final concentration of 600 mg / L (K2) of potassium dihydrogen phosphate.

[0120] Take an appropriate amount of spermidine, 2,4-epibrassinolide, potassium dihydrogen phosphate, and Tween-20 to prepare a solution with final concentrations of 200 mg / L, 0.05 mg / L, and 600 mg / L (Spd4 + EBR3 + K2).

[0121] Select the winter wheat variety Weilong 169 as the material at Caoxinzhuang Farm, Yangling Demonstration Area, Shaanxi Province (34°18′N, 108°06′E). Under drought stress (DS) (soil relative humidity 50 - 55%) during the booting stage of wheat growth, spermidine (Spd), 2,4-epibrassinolide (EBR), and potassium dihydrogen phosphate (K) were sprayed, with spraying of clear water as the control, that is:

[0122] Drought stress + spraying of clear water (DS);

[0123] Drought stress + spraying of 200 mg / L spermidine (Spd4);

[0124] Drought stress + spraying of 0.05 mg / L 2,4-epibrassinolide (EBR3);

[0125] Drought stress + spraying 600 mg / L potassium dihydrogen phosphate (K2);

[0126] Drought stress + spraying a solution of 200 mg / L spermidine + 0.05 mg / L 2,4-epibrassinolide + 600 mg / L potassium dihydrogen phosphate (Spd4+EBR3+K2).

[0127] The experiment adopted a split-plot design, and the area of each plot was 6 m 2 (2 m × 3 m). Starting 5 days before the booting stage, the above spraying was continuously carried out for 2 days at 16:00 - 18:00 in the afternoon on sunny days, making the leaves completely wet but the liquid not flowing down, that is, the spraying amount was 75 ml / m 2 .

[0128] Example 6

[0129] Comparison of the drought-resistant growth regulator of this application and its individual active ingredients in improving the drought resistance of wheat and stabilizing grain yield.

[0130] The active ingredients of the growth regulator in this example were counted by mass fraction: 1 part of spermidine, 0.0001 part of 2,4-epibrassinolide, and 11 parts of potassium dihydrogen phosphate.

[0131] Take an appropriate amount of spermidine and 0.1 ml of Tween-20 to prepare a solution with a final concentration of 50 mg / L of spermidine (Spd1).

[0132] Take an appropriate amount of 2,4-epibrassinolide and 0.1 ml of Tween-20 to prepare a solution with a final concentration of 0.005 mg / L of 2,4-epibrassinolide (EBR1).

[0133] Take an appropriate amount of potassium dihydrogen phosphate and 0.1 ml of Tween-20 to prepare a solution with a final concentration of 550 mg / L of potassium dihydrogen phosphate (K1).

[0134] Take an appropriate amount of spermidine, 2,4-epibrassinolide, potassium dihydrogen phosphate and Tween-20 to prepare a solution with final concentrations of 50 mg / L, 0.005 mg / L, 550 mg / L (Spd1+EBR1+K1).

[0135] Select the winter wheat variety Weilong 169 as the material, at Caoxinzhuang Farm, Yangling Demonstration Area, Shaanxi Province (34°18′N, 108°06′E). Under the condition of drought stress (DS) (soil relative humidity 50 - 55%) during the booting stage of wheat growth, spermidine (Spd), 2,4-epibrassinolide (EBR), and potassium dihydrogen phosphate (K) were sprayed, with spraying clear water as the control, that is:

[0136] Drought stress + spraying clear water (DS);

[0137] Drought stress + spraying 50 mg / L spermidine (Spd4);

[0138] Drought stress + spraying 0.005 mg / L 2,4-epibrassinolide (EBR3);

[0139] Drought stress + spraying 550 mg / L potassium dihydrogen phosphate (K2);

[0140] Drought stress + spraying a solution of 50 mg / L spermidine + 0.005 mg / L 2,4-epibrassinolide + 550 mg / L potassium dihydrogen phosphate (Spd1 + EBR1 + K1).

[0141] The experiment adopted a split-plot experimental design, and the area of each plot was 6 m 2 (2 m × 3 m). Starting 5 days before the booting stage, the above spraying was continuously carried out for 2 days at 16:00 - 18:00 in the afternoon on sunny days, so that the leaves were completely wetted but the liquid did not flow down, that is, the spraying amount was 75 ml / m 2 .

[0142] The experimental results show that the drought-resistant growth regulator and its active ingredients in this example can improve the drought resistance of wheat and stabilize the grain yield. The results of improving the drought resistance of wheat are as Figure 11-12 shown. The drought-resistant growth regulator increased the 1000-grain weight, grain number per spike, yield, chlorophyll content and net photosynthetic rate of wheat compared with the single application of spermidine, 2,4-epibrassinolide, and potassium dihydrogen phosphate under drought. The highest yield under drought after spraying the drought-resistant growth regulator reached 7.44 t / hm 2 , which was significantly increased by 13.49% - 14.00% compared with the single application of spermidine, 2,4-epibrassinolide, and potassium dihydrogen phosphate under drought.

[0143] Example 7

[0144] Comparison of the drought-resistant growth regulator of this application and its individual active ingredients in improving the drought resistance of wheat and stabilizing the grain yield.

[0145] The active ingredients of the growth regulator in this example are calculated by mass fraction as follows: 5 parts of spermidine, 0.004 parts of 2,4-epibrassinolide, and 15 parts of potassium dihydrogen phosphate.

[0146] Take an appropriate amount of spermidine and 0.1 ml of Tween-20 to prepare a solution with a final concentration of 250 mg / L of spermidine (Spd5).

[0147] Take an appropriate amount of 2,4-epibrassinolide and 0.1 ml of Tween-20 to prepare a solution with a final concentration of 0.2 mg / L of 2,4-epibrassinolide (EBR5).

[0148] Take an appropriate amount of potassium dihydrogen phosphate and 0.1 ml of Tween-20, and prepare a solution with a final concentration of 750 mg / L (K5) of potassium dihydrogen phosphate.

[0149] Take an appropriate amount of spermidine, 2,4-epibrassinolide, potassium dihydrogen phosphate and Tween-20, and prepare a solution with final concentrations of 250 mg / L, 0.2 mg / L, and 750 mg / L (Spd5+EBR5+K5).

[0150] Select the winter wheat variety Weilong 169 as the material at Caoxinzhuang Farm, Yangling Demonstration Area, Shaanxi Province (34°18′N, 108°06′E). During the booting stage of wheat growth, under drought stress (DS) (soil relative humidity 50-55%), spray spermidine (Spd), 2,4-epibrassinolide (EBR), and potassium dihydrogen phosphate (K), with spraying water as the control, namely:

[0151] Drought stress + spraying water (DS);

[0152] Drought stress + spraying 250 mg / L spermidine (Spd5);

[0153] Drought stress + spraying 0.2 mg / L 2,4-epibrassinolide (EBR5);

[0154] Drought stress + spraying 750 mg / L potassium dihydrogen phosphate (K5);

[0155] Drought stress + spraying a solution of 250 mg / L spermidine + 0.2 mg / L 2,4-epibrassinolide + 750 mg / L potassium dihydrogen phosphate (Spd5+EBR5+K5).

[0156] The experiment adopted a split-plot experimental design, and the area of each plot was 6 m 2 (2 m×3 m). Starting 5 days before the booting stage, continuously carry out the above spraying for 2 days at 16:00-18:00 on sunny afternoons, so that the leaves are completely wet but the liquid does not flow down, that is, the spraying amount is 75 ml / m 2 . <x

[0157] The experimental results show that both the drought-resistant growth regulator and its active ingredients in this example can improve the drought resistance of wheat and stabilize the grain yield. The results of improving the drought resistance of wheat are as Figure 13-14 shown. The drought-resistant growth regulator increased the 1000-grain weight, grain number per spike, yield, chlorophyll content, and net photosynthetic rate of wheat compared with the single application of spermidine, 2,4-epibrassinolide, and potassium dihydrogen phosphate under drought. The highest yield under drought after spraying the drought-resistant growth regulator reached 6.97 t / hm2, which was significantly increased by 7.78%-14.23% compared with the single application of spermidine, 2,4-epibrassinolide, and potassium dihydrogen phosphate under drought.

[0158] Example 8

[0159] Comparison of the drought-resistant growth regulator of this application and its individual active ingredients in improving the drought resistance of wheat and stabilizing grain yield.

[0160] The active ingredients of the growth regulator in this example are as follows by mass fraction: spermidine 4 parts, 2,4-epibrassinolide 0.001 part, potassium dihydrogen phosphate 12 parts.

[0161] Take an appropriate amount of spermidine and 0.1 ml of Tween-20 to prepare a solution with a final concentration of 100 mg / L (Spd2) of spermidine.

[0162] Take an appropriate amount of 2,4-epibrassinolide and 0.1 ml of Tween-20 to prepare a solution with a final concentration of 0.01 mg / L (EBR2) of 2,4-epibrassinolide.

[0163] Take an appropriate amount of potassium dihydrogen phosphate and 0.1 ml of Tween-20 to prepare a solution with a final concentration of 600 mg / L (K2) of potassium dihydrogen phosphate.

[0164] Take an appropriate amount of spermidine, 2,4-epibrassinolide, potassium dihydrogen phosphate and Tween-20 to prepare solutions with final concentrations of 100 mg / L, 0.01 mg / L (Spd2+EBR2), 100 mg / L, 600 mg / L (Spd2+K2), 0.01 mg / L, 600 mg / L (EBR2+K2), 100 mg / L, 0.01 mg / L, 600 mg / L (Spd2+EBR2+K2).

[0165] Select the winter wheat variety Weilong 169 as the material at Caoxinzhuang Farm, Yangling Demonstration Area, Shaanxi Province (34°18′N, 108°06′E). Under the condition of drought stress (DS) (soil relative humidity 50-55%) during the booting stage of wheat growth, spray spermidine (Spd), 2,4-epibrassinolide (EBR), potassium dihydrogen phosphate (K) in pairwise combination or the drought-resistant growth regulator of this application, with spraying clear water as the control, that is:

[0166] Drought stress + spraying clear water (DS);

[0167] Drought stress + spraying 100 mg / L spermidine + 0.01 mg / L 2,4-epibrassinolide (Spd2+EBR2);

[0168] Drought stress + spraying 100 mg / L spermidine + 600 mg / L potassium dihydrogen phosphate (Spd2+K2);

[0169] Drought stress + spraying 0.01 mg / L 2,4-epibrassinolide + 600 mg / L potassium dihydrogen phosphate (EBR2 + K2);

[0170] Drought stress + spraying 100 mg / L spermidine + 0.01 mg / L 2,4-epibrassinolide + 600 mg / L potassium dihydrogen phosphate (Spd2 + EBR2 + K2) solution.

[0171] The experiment adopted a split-plot experimental design, and the area of each plot was 6 m 2 (2 m × 3 m). Starting 5 days before the booting stage, the above spraying was continuously carried out for 2 days at 16:00 - 18:00 in the afternoon on sunny days, so that the leaves were completely wet, but the liquid did not flow down, that is, the spraying amount was 75 ml / m 2 .

[0172] The experimental results showed that the drought-resistant growth regulator of this example, and the binary compounding of spermidine, 2,4-epibrassinolide, and potassium dihydrogen phosphate improved the drought resistance of wheat. The results were as Figure 15-16 shown. Compared with the binary compounding of spermidine, 2,4-epibrassinolide, and potassium dihydrogen phosphate, the drought-resistant growth regulator more significantly increased the 1000-grain weight, number of grains per spike, yield, chlorophyll content, and net photosynthetic rate of wheat. Under drought conditions, the highest yield of spraying the drought-resistant growth regulator reached 7.55 t / hm 2 , which was significantly increased by 24.81% - 27.19% compared with spraying the binary compounding agent.

[0173] Example 9

[0174] Comparison of the drought-resistant growth regulator of this application and its individual active ingredients in improving the drought resistance of wheat and stabilizing grain yield.

[0175] The active ingredients of the growth regulator in this example were counted by mass fraction as: 3 parts of spermidine, 0.001 part of 2,4-epibrassinolide, and 13 parts of potassium dihydrogen phosphate.

[0176] Take an appropriate amount of spermidine and 0.1 ml of Tween-20 to prepare a solution with a final concentration of spermidine of 150 mg / L (Spd3).

[0177] Take an appropriate amount of 2,4-epibrassinolide and 0.1 ml of Tween-20 to prepare a solution with a final concentration of 2,4-epibrassinolide of 0.05 mg / L (EBR3).

[0178] Take an appropriate amount of potassium dihydrogen phosphate and 0.1 ml of Tween-20 to prepare a solution with a final concentration of potassium dihydrogen phosphate of 650 mg / L (K3).

[0179] Take appropriate amounts of spermidine, 2,4-epibrassinolide, potassium dihydrogen phosphate, and Tween-20, and prepare solutions with final concentrations of 150 mg / L, 0.05 mg / L (Spd3 + EBR3), 150 mg / L, 650 mg / L (Spd3 + K3), 0.05 mg / L, 650 mg / L (EBR3 + K3), and 150 mg / L, 0.05 mg / L, 650 mg / L (Spd3 + EBR3 + K3).

[0180] Select the winter wheat variety Weilong 169 as the material at Caoxinzhuang Farm, Yangling Demonstration Area, Shaanxi Province (34°18′N, 108°06′E). Under drought stress (DS) (soil relative humidity 50 - 55%) during the booting stage of wheat growth, spray spermidine (Spd), 2,4-epibrassinolide (EBR), potassium dihydrogen phosphate (K) in pairwise combinations or the drought-resistant growth regulator of the present application, with spraying of clear water as the control, namely:

[0181] Drought stress + spraying of clear water (DS);

[0182] Drought stress + spraying of 150 mg / L spermidine + 0.05 mg / L 2,4-epibrassinolide (Spd3 + EBR3);

[0183] Drought stress + spraying of 150 mg / L spermidine + 650 mg / L potassium dihydrogen phosphate (Spd3 + K3);

[0184] Drought stress + spraying of 0.05 mg / L 2,4-epibrassinolide + 650 mg / L potassium dihydrogen phosphate (EBR3 + K3);

[0185] Drought stress + spraying of a solution of 150 mg / L spermidine + 0.05 mg / L 2,4-epibrassinolide + 650 mg / L potassium dihydrogen phosphate (Spd3 + EBR3 + K3).

[0186] The experiment adopted a split-plot experimental design, and the area of each plot was 6 m 2 (2 m × 3 m). Starting 5 days before the booting stage, continuously conduct the above spraying for 2 days at 16:00 - 18:00 in the afternoon on sunny days, so that the leaves are completely wet but the liquid does not flow down, that is, the spraying amount is 75 ml / m 2 .

[0187] The experimental results show that the drought-resistant growth regulator of this example and the pairwise combinations of spermidine, 2,4-epibrassinolide, and potassium dihydrogen phosphate improve the drought resistance of wheat as follows Figure 17-18As shown, compared with the binary combinations of spermidine, 2,4-epibrassinolide, and potassium dihydrogen phosphate, the drought-resistant growth regulator more significantly increased the 1000-grain weight, number of grains per spike, yield, chlorophyll content, and net photosynthetic rate of wheat. Under drought stress, the highest yield obtained by spraying the drought-resistant growth regulator reached 7.91 t / hm 2 , which was significantly increased by 24.06%-30.50% compared with the binary combination agents under dry spraying.

[0188] Example 10

[0189] Comparison of the drought-resistant growth regulator of this application with its individual active ingredients in improving the drought resistance of wheat and stabilizing grain yield.

[0190] The active ingredients of the growth regulator in this example were as follows by mass fraction: 4 parts of spermidine, 0.002 parts of 2,4-epibrassinolide, and 14 parts of potassium dihydrogen phosphate.

[0191] Take an appropriate amount of spermidine and 0.1 ml of Tween-20 to prepare a solution with a final concentration of 200 mg / L (Spd4) of spermidine.

[0192] Take an appropriate amount of 2,4-epibrassinolide and 0.1 ml of Tween-20 to prepare a solution with a final concentration of 0.1 mg / L (EBR4) of 2,4-epibrassinolide.

[0193] Take an appropriate amount of potassium dihydrogen phosphate and 0.1 ml of Tween-20 to prepare a solution with a final concentration of 700 mg / L (K4) of potassium dihydrogen phosphate.

[0194] Take appropriate amounts of spermidine, 2,4-epibrassinolide, potassium dihydrogen phosphate, and Tween-20 to prepare solutions with final concentrations of 200 mg / L, 0.10 mg / L (Spd4+EBR4), 200 mg / L, 700 mg / L (Spd4+K4), 0.10 mg / L, 700 mg / L (EBR4+K4), and 200 mg / L, 0.10 mg / L, 700 mg / L (Spd4+EBR4+K4).

[0195] Select the winter wheat variety Weilong 169 as the material at Caoxinzhuang Farm, Yangling Demonstration Area, Shaanxi Province (34°18′N, 108°06′E). Under the condition of drought stress (DS) (soil relative humidity 50-55%) during the booting stage of wheat growth, spray spermidine (Spd), 2,4-epibrassinolide (EBR), potassium dihydrogen phosphate (K) in binary combinations or the drought-resistant growth regulator of this application, with spraying water as the control, that is:

[0196] Drought stress + spraying 250 mg / L spermidine + 0.10 mg / L 2,4-epibrassinolide (Spd4+EBR4);

[0197] Drought stress + spraying 200 mg / L spermidine + 700 mg / L potassium dihydrogen phosphate (Spd4+K4);

[0198] Drought stress + spraying 0.10 mg / L 2,4-epibrassinolide + 700 mg / L potassium dihydrogen phosphate (EBR4+K4);

[0199] A solution of drought stress + spraying 200 mg / L spermidine + 0.10 mg / L 2,4-epibrassinolide + 700 mg / L potassium dihydrogen phosphate (Spd4+EBR4+K4).

[0200] The experiment adopted a split-plot experimental design, and the area of each plot was 6 m 2 (2 m×3 m). Starting 5 days before the booting stage, the above spraying was continuously carried out for 2 days at 16:00-18:00 in the afternoon on sunny days, so that the leaves were completely wet, but the liquid did not flow down, that is, the spraying amount was 75 ml / m 2 .

[0201] The experimental results show that the drought-resistant growth regulator of this embodiment and the binary compounding of spermidine, 2,4-epibrassinolide, and potassium dihydrogen phosphate improve the drought resistance of wheat as Figure 19-20 shown. Compared with the binary compounding of spermidine, 2,4-epibrassinolide, and potassium dihydrogen phosphate, the drought-resistant growth regulator more significantly improves the 1000-grain weight, spike grain number, yield, chlorophyll content, and net photosynthetic rate of wheat. The highest yield of spraying the drought-resistant growth regulator under drought reached 7.63 t / hm 2 , which was significantly increased by 26.71%-36.40% compared with spraying the binary compounding agent.

[0202] After considering the specification and the invention disclosed herein in practice, those skilled in the art will readily think of other embodiments of the present disclosure. This application is intended to cover any variations, uses, or adaptations of the present disclosure, which follow the general principles of the present disclosure and include the common general knowledge or conventional technical means in the technical field not disclosed in the present disclosure. The specification and examples are only regarded as exemplary, and the true scope and spirit of the present disclosure are pointed out by the appended claims.

Claims

1. A composition for improving the drought resistance of wheat at the booting stage, characterized in that, By mass parts, its composition components are: 1-5 parts of spermidine, 0.0001-0.004 parts of 2,4-epibrassinolide, and 11-15 parts of potassium dihydrogen phosphate.

2. The composition for improving the drought resistance of wheat at the booting stage according to claim 1, wherein By mass parts, its composition components are: 2-4 parts of spermidine, 0.0002-0.002 parts of 2,4-epibrassinolide, and 12-14 parts of potassium dihydrogen phosphate.

3. A growth regulator for improving the drought resistance of wheat at the booting stage, characterized in that, Its active ingredient is the composition described in any one of claims 1-2.

4. The growth regulator for improving the drought resistance of wheat at the booting stage according to claim 3, wherein It further contains a pesticidally acceptable surfactant and an adhesive.

5. The growth regulator for improving the drought resistance of wheat at the booting stage according to claim 4, wherein The surfactant and the adhesive are Tween-20.

6. The growth regulator for improving the drought resistance of wheat at the booting stage according to claim 4, wherein, The ratio of spermidine to the surfactant and the adhesive is 50-250 mg:0.1 ml.

7. The growth regulator for improving the drought resistance of wheat at the booting stage according to claim 4, wherein, The ratio of spermidine to the surfactant and the adhesive is 100-200 mg:0.1 ml.

8. A preparation method of a growth regulator for improving the drought resistance of wheat at the booting stage, characterized in that, For preparing the growth regulator for improving the drought resistance of wheat at the booting stage described in any one of claims 3-7, it includes: dissolving and mixing spermidine, brassinolide, and potassium dihydrogen phosphate with clear water containing Tween-20 to obtain the product.

9. Use of the composition described in any one of claims 1-2, or the growth regulator described in any one of claims 3-7, in improving the drought resistance of wheat at the booting stage.

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