Substance and method for improving salt tolerance of cotton in seedling stage and application

Abstract

The invention discloses a substance and a method for improving salt tolerance of cotton in a seedling stage and application of the substance and the method, solves the technical problem of improving the salt tolerance of the cotton in the seedling stage, and particularly discloses application of glutathione or salt of the glutathione or solvates of the glutathione or the salt of the glutathione in improving the salt tolerance of plants. The salt tolerance of the cotton in the seedling stage can be improved.

Description

Technical Field

[0001] The present invention specifically relates to a substance, method and application for improving the salt tolerance of cotton seedlings. Background Art

[0002] According to the statistics of the Food and Agriculture Organization, the global area of land affected by salt exceeds 1 billion hectares, equivalent to 24% of the world's arable land area. Effectively utilizing these saline-alkali lands will be a huge resource for agricultural development. NaCl and Na2SO4 are the main soluble salts in the soil. When the soil salt concentration reaches a certain level, it will cause osmotic stress and nutrient imbalance. Generally, when the soil salt content exceeds 0.2% to 0.5%, it is difficult for plants to absorb water. When the salt content exceeds 0.4%, plant cells lose water osmotically, and the growth rate decreases significantly, even leading to plant death. In addition, when the Na + value is too high, the absorption of K + will be reduced, and at the same time, the absorption of PO4 3- and Ga 2+ will be inhibited. The damage of salts will disrupt the normal metabolism of plants, reduce their photosynthesis, inhibit respiration and protein synthesis, along with the toxicity of reactive oxygen species to cells, ultimately leading to DNA damage and reactive oxygen species-mediated cell death, resulting in poor leaf growth, inhibited root growth, and tissue blackening and necrosis. How to enhance the productivity potential of saline-alkali lands? This problem not only represents a frontier scientific challenge in international research but also an important issue for sustainable agricultural development.

[0003] Cotton is one of the most important economic crops globally and also a pioneer crop for improving and restoring saline-alkali lands. Therefore, increasing the yield of cotton in saline-alkali lands is an urgent problem to be solved in this field. Summary of the Invention

[0004] The technical problem solved by the present invention is to improve the salt tolerance of Gossypium plants. Using glutathione for Gossypium plants can improve the salt tolerance of Gossypium plants, especially the germination rate and radicle length under salt stress conditions.

[0005] In a first aspect, the present invention provides a plant growth regulating composition, which comprises glutathione or its salt, or their solvates.

[0006] In another aspect, the present invention provides a method for improving the salt tolerance of Gossypium plants, which comprises the step of applying glutathione, its salt, or the above-mentioned plant growth regulating composition to Gossypium plants during the cultivation of Gossypium plants.

[0007] In another aspect, the present invention provides a cultivation method for Gossypium plants, which comprises the step of applying glutathione, its salt, or the above-mentioned plant growth regulating composition to Gossypium plants.

[0008] In a specific embodiment, application is selected from root drenching, foliar spraying, atomizing, composting, seed soaking, coating, field flooding irrigation, soilless cultivation, drip irrigation of plants or plant organs, smearing of plants or plant organs, dripping of plants or plant organs, or any combination thereof.

[0009] In a specific embodiment, the soilless cultivation is hydroponics. In a specific embodiment, the application concentration of glutathione is 1 mM - 20 mM;

[0010] and / or, the application time of glutathione is 1 - 24 hours.

[0011] In certain embodiments, the salts thereof include hydrochloride, sulfate, phosphate, nitrate, acetate, citrate, maleate, tartrate, mesylate, benzenesulfonate, p-toluenesulfonate, fumarate, lactate, gluconate, or any combination thereof.

[0012] In certain embodiments, the application time of glutathione is 1 hour, 2 hours, 3 hours, 4 hours, 5 hours, 6 hours, 7 hours, 8 hours, 9 hours, 10 hours, 11 hours, 12 hours, 13 hours, 14 hours, 15 hours, 16 hours, 17 hours, 18 hours, 19 hours, 20 hours, 21 hours, 22 hours, 23 hours, 24 hours.

[0013] In certain embodiments, the application concentration of glutathione is 1 mM, 2 mM, 3 mM, 4 mM, 5 mM, 6 mM, 7 mM, 8 mM, 9 mM, 10 mM, 11 mM, 12 mM, 13 mM, 14 mM, 15 mM, 16 mM, 17 mM, 18 mM, 19 mM, 20 mM.

[0014] In certain embodiments, the cotton plants to which the growth regulating composition is applied have higher salt tolerance compared to cotton plants to which the growth regulating composition is not applied.

[0015] In certain embodiments, the salt tolerance is seed salt tolerance or seedling stage salt tolerance.

[0016] In certain embodiments, the salt tolerance includes germination rate and / or radicle length.

[0017] In certain embodiments, the salt tolerance is the tolerance ability of cotton plants to salt injury or salt stress.

[0018] In certain embodiments, the salt stress is NaCl stress.

[0019] In a specific embodiment, the NaCl concentration in the NaCl stress is 200 mM.

[0020] In some embodiments, the tolerance is the germination rate and / or the radicle length tolerance. In a specific embodiment, the seedling stage is cultured for 1, 2, and / or 3 days.

[0021] On the other hand, the present invention provides the use of glutathione or its salt, or their solvate, in improving the salt tolerance of Gossypium plants.

[0022] In a specific embodiment, the use includes improving the germination rate and / or the radicle length.

[0023] On the other hand, the present invention provides a plant growth regulating composition, which comprises glutathione or its salt, or their solvate.

[0024] In some embodiments, the composition is formulated into a solid preparation or a liquid preparation.

[0025] In some embodiments, the Gossypium plants treated with the liquid preparation, the solid preparation, and the growth regulator have higher salt tolerance than the Gossypium plants not treated with the composition, the liquid preparation, the solid preparation, and the growth regulator.

[0026] In some embodiments, the Gossypium plants are selected from Gossypium herbaceum, Gossypium arboreum, Gossypium anomalum, Gossypium triphyllum, Gossypium davidsonii, Gossypium stocksii, Gossypium australe, Gossypium bickii, Gossypium armourianum, Gossypium thurberi, Gossypium laxum, Gossypium sturtianum, Gossypium somalense, Gossypium incanum, Gossypium longicalyx, Gossypium bickii, Gossypium barbadense, Gossypium hirsutum, Gossypium tomentosum, Gossypium mustelinum, Gossypium darwinii, Gossypium lanceolatum, or any combination thereof.

[0027] In some embodiments, the Gossypium plants are selected from Zhong 9807, Zhong S9612, Jimian 27, CCRI 23, Han 109, GK50, Lumianyan 21, Xinluzao 66, or any combination thereof.

[0028] On the other hand, the present invention provides a plant growth regulating composition, which comprises glutathione or its salt, or their solvate, or any combination thereof;

[0029] In a specific embodiment, the composition is used for plant seeds or seedling stage plants;

[0030] In a specific embodiment, the seedling stage plants are 3 - 3.5 day plants;

[0031] In a specific embodiment, the composition is formulated into a solid preparation and a liquid preparation;

[0032] In a specific embodiment, it further comprises excipients and / or additives;

[0033] In a specific embodiment, the adjuvants include agriculturally or horticulturally acceptable diluents, fillers, solvents, self-promoters, carriers, emulsifiers, dispersants, thickeners, binders, or any combination thereof;

[0034] In a specific embodiment, the additives include agriculturally or horticulturally acceptable preservatives, adjuvants, synergists, microbial additives, antifreeze agents, or any combination thereof.

[0035] More preferably, the dispersant and / or emulsifier includes all non-ionic, anionic or cationic dispersants conventionally used in formulations of active agrochemical ingredients;

[0036] More preferably, the preservatives include, but are not limited to, dichlorophen and benzyl alcohol semi-formal;

[0037] More preferably, the binders include, but are not limited to, starch, polyvinyl alcohol, and gelatin;

[0038] More preferably, the dispersants include, but are not limited to, sodium dodecylbenzenesulfonate and sodium butylnaphthalenesulfonate;

[0039] More preferably, the stabilizers include, but are not limited to, silica, talc powder, and dicyandiamide;

[0040] More preferably, the synergists include, but are not limited to, sodium nitrophenolate and vitamin C;

[0041] More preferably, the fillers include, but are not limited to, volcanic stone powder and ammonium bicarbonate;

[0042] More preferably, the osmotic regulators include, but are not limited to, betaine, proline, and soluble sugars;

[0043] More preferably, examples of agriculturally or horticulturally acceptable diluents include monosaccharides, polysaccharides, molasses, gums, lignosulfonates, aqueous solutions of glycerol, sorbitol, and propylene glycol, water, vegetable oils, and mineral oils;

[0044] More preferably, the carriers include, but are not limited to, alginate beads, durum wheat flour granules, silica, clay, clay minerals, gelatin, cellulose, cellulose derivatives, calcium chlorite, talc powder, diatomaceous earth, activated carbon, animal bone charcoal, peat, vermiculite, lignite, wood chips, and / or corn cobs.

[0045] In a specific embodiment, the composition further includes one or more chemical drugs;

[0046] In a specific embodiment, it is selected from plant hormones, osmotic regulators, mineral elements and inorganic salts, organic modifiers, microbial agents, nanomaterials, antioxidants, agricultural modifiers, or any combination thereof;

[0047] In a specific embodiment, the phytohormone is selected from abscisic acid, brassinolide, salicylic acid, jasmonic acid, ethylene inhibitor, gibberellin, or any combination thereof;

[0048] In a specific embodiment, the osmotic regulator is selected from betaine, proline, soluble sugar, sorbitol, glycine, or any combination thereof;

[0049] In a specific embodiment, the mineral elements and inorganic salts are selected from calcium salts (such as CaCl2, Ca(NO3)2), silicon fertilizers (such as sodium silicate, potassium silicate), potassium fertilizers (such as KNO3, K2SO4), magnesium (such as MgSO4), sulfur (such as S element or sulfur-containing compounds), zinc, molybdenum, or any combination thereof;

[0050] In a specific embodiment, the organic modifier is selected from humic acid, seaweed extract, chitosan, polyamines (such as spermidine, putrescine, spermine), biochar, amino acid mixture (such as glutamic acid, proline), or any combination thereof;

[0051] In a specific embodiment, the microbial preparation is selected from salt-tolerant rhizosphere bacteria (such as Bacillus, Pseudomonas), arbuscular mycorrhizal fungi, cyanobacteria (such as Nostoc), yeasts, or any combination thereof;

[0052] In a specific embodiment, the nanomaterial is selected from nano-silicon (such as SiO2 NPs), nano-zinc oxide (such as ZnO NPs), nano-iron (Fe3O4 NPs), carbon nanotubes, or any combination thereof;

[0053] In a specific embodiment, the antioxidant is selected from ascorbic acid (such as vitamin C), α-tocopherol (such as vitamin E), or any combination thereof;

[0054] In a specific embodiment, the agricultural modifier is selected from gypsum (such as CaSO4·2H2O), organic fertilizers (such as compost, green manure), hydrogels (such as polyacrylamide), or any combination thereof.

[0055] In a specific embodiment, the composition is a liquid preparation;

[0056] Preferably, the liquid preparation includes emulsifiable concentrate, soluble solution, oil agent, dispersible liquid agent, electrostatic spraying liquid agent, suspending agent, microcapsule suspending agent, oil suspending agent, suspension emulsion, water emulsion, microemulsion, nanoemulsion, or any combination thereof;

[0057] In a specific embodiment, in the liquid preparation, the glutathione is in an agriculturally or fertilizer-chemically acceptable carrier;

[0058] In a specific embodiment, in the liquid preparation, the concentration of glutathione is 1 mM - 20 mM;

[0059] In a specific embodiment, the concentration of glutathione is 10 mM;

[0060] In a specific embodiment, the liquid formulation is used for root soaking, foliar spraying, spraying, composting, seed soaking, coating, field flooding, drip irrigation of plants or plant organs, painting plants or plant organs, dripping plants or plant organs, or any combination thereof.

[0061] In a specific embodiment, the composition is a solid preparation;

[0062] In a specific embodiment, in the solid preparation, the glutathione is in an agriculturally or fertilizer-acceptable carrier;

[0063] In a specific embodiment, the solid formulation is used for root soaking, foliar spraying, spraying, composting, seed soaking, coating, field flooding, drip irrigation of plants or plant organs, smearing plants or plant organs, dripping plants or plant organs, or any combination thereof;

[0064] In a specific embodiment, the powdered fertilizer is used to prepare the above-mentioned liquid preparation.

[0065] In a specific embodiment, the solid preparation or liquid preparation is selected from a foliar treatment agent, a soil treatment agent, a seed treatment agent, a stem treatment agent, a root treatment agent, or any combination thereof;

[0066] In a specific embodiment, the plant is a plant seed or a seedling plant;

[0067] In a specific embodiment, the seedling plants are 3-3.5 day old plants.

[0068] On the other hand, the present invention provides a method for improving salt tolerance of cotton plants, the method comprising applying glutathione, a salt thereof, or the above-mentioned composition to the cotton plants during cultivation of the cotton plants;

[0069] In a specific embodiment, the applying comprises root dipping, foliar spraying, spraying, composting, seed soaking, coating, field flooding, drip irrigation of plants or plant organs, painting plants or plant organs, dripping plants or plant organs;

[0070] In a specific embodiment, the glutathione concentration of the seed soaking is 1mM-20mM;

[0071] In a specific embodiment, the glutathione concentration is 10 mM;

[0072] In a specific embodiment, the soaking time is 1-24 hours;

[0073] In a specific embodiment, the soaking time is 12 hours;

[0074] In a specific embodiment, the cotton plants to which the glutathione, its salts, or the composition is applied have higher salt tolerance compared to cotton plants to which the glutathione, its salts, or the composition is not applied;

[0075] In a specific embodiment, the higher salt tolerance means having a higher germination rate and / or radicle length under salt stress conditions;

[0076] In a specific embodiment, the cotton plants are cotton plant seeds and / or cotton plants at the seedling stage;

[0077] In a specific embodiment, the salt tolerance is seed salt tolerance or seedling salt tolerance;

[0078] In a specific embodiment, the seedling stage is 3 - 3.5 days;

[0079] In a specific embodiment, the salt stress is NaCl stress;

[0080] In a specific embodiment, the concentration of NaCl is 200 mM;

[0081] In a specific embodiment, the salts thereof are selected from sodium salts, potassium salts, calcium salts, magnesium salts, zinc salts, lithium salts, hydrochloride salts, sulfate salts, arginine salts, nanoparticle-loaded salts, or any combination thereof.

[0082] On the other hand, the present invention provides a method for cultivating cotton plants, the method comprising the step of applying the above composition to cotton plants to obtain cotton plants having roots, stems, leaves, flowers, fruits, seeds, and cotton bolls;

[0083] In a specific embodiment, the application includes root soaking, foliar spraying, atomization, composting, seed soaking, coating, field flooding irrigation, drip irrigation of plants or plant organs, smearing of plants or plant organs, and dropping of plants or plant organs;

[0084] In a specific embodiment, the concentration of glutathione for seed soaking is 1 mM - 20 mM;

[0085] In a specific embodiment, the concentration of glutathione is 10 mM;

[0086] In a specific embodiment, the time for seed soaking is 1 - 24 h;

[0087] In a specific embodiment, the time for seed soaking is 12 h;

[0088] In a specific embodiment, the cotton plants to which the glutathione, its salts, or the composition is applied have higher salt tolerance compared to cotton plants to which the glutathione, its salts, or the composition is not applied;

[0089] In a specific embodiment, the higher salt tolerance means having a higher germination rate and / or radicle length under salt stress environment;

[0090] In a specific embodiment, the Gossypium plant is Gossypium plant seeds and / or Gossypium plants at the seedling stage;

[0091] In a specific embodiment, the salt tolerance is seed salt tolerance or seedling stage salt tolerance;

[0092] In a specific embodiment, the seedling stage is 3 - 3.5 days;

[0093] In a specific embodiment, the salt stress is Na + stress, Cl - stress and / or SO4 2- stress;

[0094] In a specific embodiment, the salt stress is NaCl stress.

[0095] In a specific embodiment, the NaCl concentration in the NaCl stress is 200 mM.

[0096] In a specific embodiment, the salt thereof is selected from sodium salts, potassium salts, calcium salts, magnesium salts, zinc salts, lithium salts, hydrochloride salts, sulfate salts, arginine salts, nanoparticle - loaded salts, or any combination thereof.

[0097] In a specific embodiment, the Gossypium plant is selected from Gossypium herbaceum, Gossypium arboreum, Gossypium anomalum, Gossypium triphyllum, Gossypium davidsonii, Gossypium stocksii, Gossypium australe, Gossypium bickii, Gossypium armourianum, Gossypium hirsutum, Gossypium barbadense, Gossypium hawaiiense, Gossypium mustelinum, Gossypium darwinii, Gossypium lanceolatum, or any combination thereof.

[0098] In a specific embodiment, the Gossypium plant is selected from Zhong 9807, Zhong S9612, Jimian 27, CCRI 23, Han 109, GK50, Lumianyan 21, Xinlu 66, or any combination thereof

[0099] On the other hand, the present invention provides the use of glutathione or its salt, or their solvates, or the above - mentioned compositions in improving the salt tolerance of plants;

[0100] In a specific embodiment, the Gossypium plant is selected from Gossypium herbaceum, Gossypium arboreum, Gossypium anomalum, Gossypium triphyllum, Gossypium davidsonii, Gossypium stocksii, Gossypium australe, Gossypium bickii, Gossypium armourianum, Gossypium hirsutum, Gossypium barbadense, Gossypium hawaiiense, Gossypium mustelinum, Gossypium darwinii, Gossypium lanceolatum, or any combination thereof;

[0101] In a specific embodiment, the cotton plant is selected from Zhong 9807, Zhong S9612, Jimian 27, CCRI 23, Han 109, GK50, Lumianyan 21, Xinluzao 66, or any combination thereof;

[0102] In a specific embodiment, the salt tolerance is seed salt tolerance or seedling salt tolerance;

[0103] In a specific embodiment, the seedling stage is 3 - 3.5 days;

[0104] In a specific embodiment, the salt stress is Na + stress, Cl - stress, and / or SO4 2- stress;

[0105] In a specific embodiment, the salt stress is NaCl stress;

[0106] In a specific embodiment, the NaCl concentration of the NaCl stress is 200 mM;

[0107] In a specific embodiment, the salt thereof is selected from sodium salts, potassium salts, calcium salts, magnesium salts, zinc salts, lithium salts, hydrochloride salts, sulfate salts, arginine salts, nanoparticle - loaded salts, or any combination thereof.

[0108] In a specific embodiment, the foliar treatment agent is selected from soluble agents, emulsifiable concentrates, suspensions, wettable powders, emulsions in water, ultra - low volume liquids, or any combination thereof;

[0109] In a specific embodiment, the soil treatment agent is selected from granulates, slow - release granulates, soil fumigants, soluble powders, or any combination thereof;

[0110] In a specific embodiment, the seed treatment agent is selected from seed treatment suspensions, seed treatment dry powders, seed treatment dispersible powders, or any combination thereof;

[0111] In a specific embodiment, the stem treatment agent is selected from coating agents, injection agents, or any combination thereof;

[0112] In a specific embodiment, the root treatment agent form is selected from irrigation granulates, water - dispersible granules, or any combination thereof.

[0113] Beneficial Effects

[0114] Cotton emergence is a critical period affecting cotton growth and development. It is necessary to provide a method for alleviating salt stress tolerance during the cotton seedling stage. The object of the present invention is to provide a method for enhancing the salt tolerance of cotton seedlings by pretreating cotton seeds with reduced glutathione (GSH), providing a reference for promoting cotton growth in saline - alkali soil and increasing the cotton yield in saline - alkali land.

[0115] The present invention discloses a substance, method and application for improving the salt tolerance of cotton seedlings at the seedling stage. The technical problem to be solved is to improve the salt tolerance of cotton seedlings at the seedling stage. Specifically, the use of glutathione or its salts, or their solvates in improving the salt tolerance of plants is disclosed. By soaking the seeds with glutathione, the salt tolerance of cotton seedlings at the seedling stage can be improved, and it can be used in agricultural production. Description of the Drawings

[0116] Figure 1 It is a phenotypic diagram of the identification results after the germination of cotton seeds under different concentrations of GSH;

[0117] Figure 2 It is a phenotypic diagram of the identification results after the germination of cotton seeds under different treatment conditions. Detailed Embodiments

[0118] The present invention will be further described in detail below in conjunction with the specific embodiments. The provided embodiments are only for clarifying the present invention, rather than limiting the scope of the present invention. The following provided embodiments can be used as a guide for those of ordinary skill in the art to make further improvements, and do not limit the present invention in any way.

[0119] In the following embodiments, unless otherwise specified, the experimental methods are all conventional methods, and are carried out according to the techniques or conditions described in the literature in this field or according to the product specifications. The materials, reagents, etc. used in the following embodiments can be obtained from commercial channels unless otherwise specified.

[0120] In the following embodiments, the SPSS 11.5 statistical software is used to process the data. The experimental results are expressed as the mean ± standard deviation, and the One-way ANOVA test is used. P < 0.05 (*) indicates a significant difference, P < 0.01 (**) indicates a highly significant difference, and P < 0.001 (***) indicates a highly significant difference.

[0121] In the present invention, unless otherwise specified, the scientific and technical terms used herein have the meanings commonly understood by those skilled in the art. And the terms related to protein and nucleic acid chemistry, molecular biology, cell and tissue culture, microbiology, immunology and the laboratory operation steps used herein are all widely used terms and conventional steps in the corresponding fields. At the same time, in order to better understand the present invention, the definitions and explanations of related terms are provided below.

[0122] As used in the present disclosure, "comprising", "including", "having", "containing", etc. are all open-ended terms, that is, they are intended to include but not limited to.

[0123] As used in this disclosure, "first" and "second" are for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, features defined as "first" and "second" may explicitly or implicitly include one or more of such features. Further, in the description of the present invention, unless otherwise specified, "a plurality of" means two or more.

[0124] As used in this disclosure, "optionally", "optional" or "option" generally means that the subsequent event or condition may but does not necessarily occur, and this description includes the case where the event or condition occurs and the case where the event or condition does not occur.

[0125] As used in this disclosure, "salt tolerance" refers to the ability of a plant to maintain normal growth, development and reproduction under a salt stress environment. In specific embodiments, it includes, but is not limited to, seed germination rate (seed germination rate, germination speed (germination index), radicle / coleoptile length, seedling survival rate after germination), seedling / adult plant growth performance (plant height, stem diameter, root length, root volume, biomass (shoot / root fresh weight, dry weight), number of leaves, leaf area, specific leaf weight (dry weight of leaves per unit area), leaf phenotype changes (wilting, chlorosis, necrosis, salt spots)), root characteristics (root-shoot ratio (ratio of root to shoot biomass), root activity (dehydrogenase activity measured by the TTC method), lateral root density, root hair development), photosynthesis and gas exchange (chlorophyll content (SPAD value or measured by spectrophotometry), photosynthetic rate (Pn), transpiration rate (Tr), stomatal conductance (Gs), intercellular CO2 concentration (Ci), photosystem II efficiency (Fv / Fm, chlorophyll fluorescence parameter), osmoregulatory substances (proline (Pro) content, soluble sugar (glucose, fructose, sucrose) content, betaine, glycine betaine content, inorganic ions (Na + , K + , Ca 2+ , Cl - ) concentration and Na + / K + ratio), antioxidant system (superoxide dismutase (SOD) activity, peroxidase (POD), catalase (CAT) activity, ascorbate peroxidase (APX) activity, malondialdehyde (MDA) content (degree of membrane lipid peroxidation), reactive oxygen species (ROS) accumulation (such as H2O2, O2 -Content), hormone response (changes in abscisic acid (ABA), ethylene (ETH) content, cytokinin (CTK), gibberellin (GA) dynamics), cell membrane stability (electrolyte permeability (conductivity method, reflecting cell membrane damage), cytoplasmic membrane permeability (Evans Blue staining method)), gene expression levels (expression levels of salt tolerance-related genes (such as SOS1, NHX1, HKT1, P5CS, BADH, etc.), transcription factor activities (such as MYB, NAC, WRKY families), signal pathway genes (such as MAPK, CDPK, CBL-CIPK pathways)), epigenetic regulation (changes in DNA methylation levels, histone modifications (such as acetylation, methylation)), proteomics (differentially expressed proteins (such as osmoregulatory proteins, antioxidant enzymes), post-translational modifications of proteins (phosphorylation, ubiquitination)), metabolomics (primary metabolites (amino acids, organic acids, sugars), secondary metabolites (flavonoids, phenols, alkaloids)), cell ultrastructure (chloroplast structure (integrity of thylakoid membranes), mitochondrial morphology (whether the cristae structure is damaged), vacuole size (ion compartmentalization ability)), cell wall properties (lignin, cellulose content, cell wall elasticity and mechanical strength), salt gland / salt bladder structure (salt gland density (such as the salt-excreting structure of halophytes), secretion ability of salt bladders), root salt excretion ability (salt concentration in root exudates, salt dynamics in rhizosphere soil), reproductive ability (flowering time, seed setting rate, seed vigor), salt tolerance index, principal component analysis, cluster analysis.

[0126] As used in this disclosure, the "seedling stage" of cotton refers to the growth stage from seed germination to the initial stage of the seedling.

[0127] As used in this disclosure, "its salts" and "pharmaceutically acceptable salts" refer to derivatives of the disclosed compounds, wherein the parent compound is modified by preparing its acid addition salts or base addition salts. Examples of its salts and pharmaceutically acceptable salts include, but are not limited to, inorganic or organic salts of basic groups such as amines; and basic salts or organic salts of acidic groups such as carboxylic acids. Pharmaceutically acceptable salts include conventional non-toxic salts or quaternary ammonium salts of the parent compound, which are prepared, for example, from non-toxic inorganic acids or non-toxic organic acids. For example, such conventional non-toxic salts include those derived from inorganic acids such as hydrochloric acid, hydrobromic acid, sulfuric acid, sulfamic acid, phosphoric acid, and nitric acid; and salts prepared from organic acids such as acetic acid, propionic acid, succinic acid, glycolic acid, stearic acid, lactic acid, malic acid, tartaric acid, citric acid, ascorbic acid, pamoic acid, maleic acid, hydroxymaleic acid, phenylacetic acid, glutamic acid, benzoic acid, salicylic acid, p-aminobenzenesulfonic acid, 2-acetoxybenzoic acid, fumaric acid, toluenesulfonic acid, methanesulfonic acid, ethanedisulfonic acid, oxalic acid, and isethionic acid.

[0128] As used in this disclosure, a "liquid preparation" refers to a homogeneous or heterogeneous system in which one or more active ingredients (such as drugs, nutrients, pesticides, bioactive substances, etc.) are dissolved, emulsified or suspended in a liquid dispersion medium, and auxiliary ingredients such as stabilizers, solubilizers, preservatives, etc. may be added. Liquid preparations include but are not limited to true solution types (where the solute is uniformly dispersed in the form of molecules or ions (particle size < 1 nm), clear and stable), colloidal solution types (where the solute is dispersed as colloidal particles (1 - 100 nm), having a certain stability and possibly showing the Tyndall effect), emulsion types (where two immiscible liquids (such as oil and water) form a stable emulsion through an emulsifier (particle size 0.1 - 100 μm)), and suspension types (where insoluble solid particles (0.5 - 10 μm) are dispersed in a liquid and a suspending agent is needed to prevent sedimentation).

[0129] As used in this disclosure, a "solid preparation" refers to a preparation form existing in a solid state. Solid preparations include but are not limited to tablets, capsules, granules, powders, pills, and films.

[0130] As used in this disclosure, a "foliar treatment agent" refers to a preparation designed for application on the surface of plant leaves. Application methods include but are not limited to spraying, smearing, and soaking.

[0131] As used in this disclosure, a "seed treatment agent" refers to a preparation designed for application on the surface of plant seeds. Application methods include but are not limited to spraying, smearing, and soaking.

[0132] As used in this disclosure, a "stem treatment agent" refers to a preparation designed for application on the surface of plant stems. Application methods include but are not limited to spraying, smearing, and soaking.

[0133] As used in this disclosure, a "root treatment agent" refers to a preparation designed for application on the surface of plant roots. Application methods include but are not limited to spraying, smearing, and soaking.

[0134] Example 1 Effects of Different Concentrations of Reduced Glutathione on the Germination Rate and Radicle Length of Cotton (Jimian 27) after Germination

[0135] (1) Material Preparation

[0136] GSH is reduced glutathione, with a CAS number of 70 - 18 - 8.

[0137] Prepare the GSH stock solution: 1.53665 g of GSH, made up to 50 ml with ddH2O.

[0138] 1 mM GSH (also known as 1 mM GSH solution): 1 ml of the stock solution, made up to 100 ml with ddH2O to make the final concentration of GSH 1 mM.

[0139] 5 mM GSH (also known as 5 mM GSH solution): 5 ml of stock solution, made up to 100 ml with ddH2O to give a final concentration of 5 mM GSH.

[0140] 10 mM GSH (also known as 10 mM GSH solution): 10 ml of stock solution, made up to 100 ml with ddH2O to give a final concentration of 10 mM GSH.

[0141] 15 mM GSH (also known as 15 mM GSH solution): 15 ml of stock solution, made up to 100 ml with ddH2O to give a final concentration of 15 mM GSH.

[0142] 20 mM GSH (also known as 20 mM GSH solution): 20 ml of stock solution, made up to 100 ml with ddH2O to give a final concentration of 20 mM GSH.

[0143] 200 mM NaCl solution: 11.688 g of NaCl, made up to 1000 ml with ddH2O to give a final concentration of 200 mM NaCl.

[0144] Randomly select 8 upland cotton materials, and the seeds are provided and preserved by the Cotton Research Institute of the Chinese Academy of Agricultural Sciences and the Cotton Research Institute of Hebei Academy of Agriculture and Forestry Sciences. The cotton seeds are delinted with concentrated sulfuric acid and air-dried, and seeds with plump grains and uniform sizes are selected.

[0145] (2) Screening of reduced glutathione (GSH) concentration

[0146] Select the cotton variety Jimian 27 to screen the concentration of GSH. The experiment is divided into 7 groups, namely 1 mM GSH group, 5 mM GSH group, 10 mM GSH group, 15 mM GSH group, 20 mM GSH group, control group and standard group. Select 30 seeds with consistent size and maturity for each group.

[0147] After soaking the 1 mM GSH group, 5 mM GSH group, 10 mM GSH group, 15 mM GSH group, 20 mM GSH group, control group, and standard group in 1 mM GSH, 5 mM GSH, 10 mM GSH, 15 mM GSH, 20 mM GSH, ddH2O, and ddH2O for 12 h in sequence. The 1 mM GSH group, 5 mM GSH group, 10 mM GSH group, 15 mM GSH group, 20 mM GSH group, and control group used the vertical double-layer filter paper method (cut the filter paper to the size of A4 paper, lay it flat on the plastic tray, place the seeds on the filter paper, arrange them in a "one" shape along the long side of the filter paper, the seeds are 2 cm away from the edge of the filter paper, and the growth points of the seeds are facing down, with a 2 cm interval between the seeds left and right. Roll up the filter paper horizontally along the position of the seeds, and the seeds should be rolled in the middle of the filter paper so that the filter paper forms a straight cylinder. Place the straight cylinder-shaped filter paper vertically in the beaker) to place the seeds in 200 mM NaCl solution for germination respectively. The standard group used the vertical double-layer filter paper method to place the seeds in ddH2O for germination respectively. Cultivate them in the dark at 28 °C for 3 d, and measure the germination rate of cotton seeds and the growth of radicles under different treatments (take pictures and record and calculate the radicle length). For the seeds treated with 10 mM GSH, when under 200 mM NaCl stress, the germination rate was significantly increased, and the radicle growth was significantly promoted, as shown in Figure ( Figure 1 in which, NaCl is the control group, GSH1, GSH5, GSH10, GSH15, GSH20 are the 1 mM GSH group, 5 mM GSH group, 10 mM GSH group, 15 mM GSH group, 20 mM GSH group respectively, and ddH2O is the standard group) and Table 1. Therefore, 10 mM GSH soaking the seeds for 12 h was selected as the treatment condition.

[0148] Germination rate (sprouting rate) = number of germinated seeds ÷ total number of seeds × 100%.

[0149] Radicle length: After the seeds germinated, the white part was regarded as the radicle length.

[0150] Table 1 Effects of different treatments on germination rate and radicle growth

[0151] Different treatments Germination rate (%) Radicle length (cm) <![CDATA[ddH2O (standard group)]]> 82.8±1.45a 10.30±0.41a NaCl (control group) 22.80±1.66b 4.51±0.17b 1 mM GSH (1 mM GSH group) 23.63±1.07b 4.90±0.13b 5 mM GSH (5 mM GSH group) 54.20±1.95c 6.85±0.10c 10 mM GSH (10 mM GSH group) 78.40±1.68d 7.60±0.25d 15 mM GSH (15 mM GSH group) 76.27±0.95d 5.62±0.14e 20 mM GSH (20 mM GSH group) 54.77±1.50c 4.76±0.19b

[0152] Example 2 Effects of 10 mM GSH reduced glutathione on germination rate and radicle length after germination of different cotton varieties

[0153] I. Material preparation

[0154] Eight upland cotton materials were randomly selected as shown in Table 2. The seeds were provided and preserved by the Cotton Research Institute of the Chinese Academy of Agricultural Sciences and the Cotton Research Institute of Hebei Academy of Agriculture and Forestry. The germplasm materials were mainly the popularized varieties in the Yellow River Basin cotton area and salt-tolerant control varieties. The cotton seeds were delinted with concentrated sulfuric acid and dried. Seeds with plump grains and uniform sizes were selected, sterilized with 75% ethanol solution for 5 min, and then repeatedly washed 3 times with ddH2O for disinfection treatment before being used in the following experiments.

[0155] Prepare the GSH stock solution: 1.53665 g of GSH, made up to 50 ml with ddH2O

[0156] 10 mM GSH (also known as 10 mM GSH solution): 10 ml of the stock solution, made up to 100 ml with ddH2O to make the final concentration of GSH 10 mM.

[0157] 200 mM NaCl solution: 11.688 g of NaCl, made up to 1000 ml with ddH2O to make the final concentration of NaCl 200 mM.

[0158] Table 2 Upland cotton materials

[0159]

[0160] Zhong 9807 is recorded in the following literature: "[1] Liu Yi. Identification and evaluation of salt tolerance at the seedling stage of 12 upland cotton varieties (lines) [J]. Crop Research, 2017".

[0161] Zhong S9612 is recorded in the following literature: "[1] Wang Junjuan, Ye Wuwei, Wang Delong, et al. Study on the germination characteristics of 41 upland cotton germplasm resources under PEG stress and their comprehensive evaluation of drought resistance [J]. Journal of Plant Genetic Resources, 2011, 12(6): 840 - 846."

[0162] GK50 is recorded in the following literature: "[1] Zhao Xiaojie. Identification and expression analysis of the VP gene family in cotton [D]. Henan University [2025 - 03 - 07]. DOI: CNKI: CDMD: 2.1017.833844."

[0163] Except for the approved varieties, the above varieties (lines) are all self-bred lines of the Stress Resistance Identification Team of the Cotton Research Institute of the Chinese Academy of Agricultural Sciences.

[0164] II. Treatment of cotton materials

[0165] The experiment was divided into 8 groups, namely the Zhong 9807 group, Zhong S9612 group, Jimian 27 group, CRI 23 group, Han 109 group, GK50 group, Lumianyan 21 group, and Xinluzao 66 group. 30 cotton seeds of the corresponding variety were selected for each group and evenly divided into 3 parts, and the following treatments were carried out in sequence:

[0166] 1) ddH2O treatment: Using the vertical double-layer filter paper method (cut the filter paper to the size of A4 paper, lay it flat on a plastic tray, moisten the filter paper with ddH2O, place the seeds on the filter paper, arrange them in a "one" shape along the long side of the filter paper, with the seeds 2 cm away from the edge of the filter paper and the growth points of the seeds facing downwards, with a 2 cm gap between the seeds left and right, roll up the filter paper horizontally along the position of the seeds, and the seeds should be rolled in the middle of the filter paper so that the filter paper forms a straight tube shape, and place the straight tube-shaped filter paper vertically in a beaker), soak the seeds in ddH2O for 12 h respectively, and then continue the treatment for 3 d.

[0167] 2) NaCl treatment (200 mM NaCl treatment): Using the vertical double-layer filter paper method (cut the filter paper to the size of A4 paper, lay it flat on a plastic tray, moisten the filter paper with 200 mM NaCl, place the seeds on the filter paper, arrange them in a "one" shape along the long side of the filter paper, with the seeds 2 cm away from the edge of the filter paper and the growth points of the seeds facing downwards, with a 2 cm gap between the seeds left and right, roll up the filter paper horizontally along the position of the seeds, and the seeds should be rolled in the middle of the filter paper so that the filter paper forms a straight tube shape, and place the straight tube-shaped filter paper vertically in a beaker), soak the seeds in 200 mM NaCl solution for 12 h respectively, and then continue the treatment for 3 d.

[0168] 3) GSH-NaCl treatment: Using the vertical double-layer filter paper method (cut the filter paper to the size of A4 paper, lay it flat on a plastic tray, moisten the filter paper with GSH solutions of different concentrations, place the seeds on the filter paper, arrange them in a "one" shape along the long side of the filter paper, with the seeds 2 cm away from the edge of the filter paper and the growth points of the seeds facing downwards, with a 2 cm gap between the seeds left and right, roll up the filter paper horizontally along the position of the seeds, and the seeds should be rolled in the middle of the filter paper so that the filter paper forms a straight tube shape, and place the straight tube-shaped filter paper vertically in a beaker), soak the seeds in 10 mM GSH for 12 h respectively, and transfer them to 200 mM NaCl solution for treatment for 3 d.

[0169] After that, measure the germination rate of cotton seeds and the growth of radicles under different treatments (take pictures and record and calculate the radicle length).

[0170] Germination rate = (Number of germinated seeds ÷ Total number of seeds) × 100%.

[0171] Radicle length: After seed germination, the white part (including the hypocotyl and radicle parts) is regarded as the radicle length here.

[0172] III. Result Evaluation

[0173] The results of statistical and differential analysis of the germination rate of seeds and the length of radicles of 8 cotton materials in three groups of treatments are shown in Table 3 and Table 4. The phenotypic identification results are as Figure 2 ( Figure 2Among them, 9807, Zhong S9612, Jimian 27, CCRI 23, Han 109, GK50, Lumianyan 21, and Xinluzao 66 are the 9807 group, Zhong S9612 group, Jimian 27 group, CCRI 23 group, Han 109 group, GK50 group, Lumianyan 21 group, and Xinluzao 66 group in sequence; ddH2O, NaCl, and GSH-NaCl are the ddH2O treatment, NaCl treatment, and GSH-NaCl treatment in sequence) as shown.

[0174] As shown in Table 3 (ddH2O is the ddH2O treatment, NaCl is the NaCl treatment, and 10 mM GSH + NaCl is the GSH-NaCl treatment), Table 4 (ddH2O is the ddH2O treatment, NaCl is the NaCl treatment, and 10 mM GSH + NaCl is the GSH-NaCl treatment), and Figure 2 It shows that 8 cotton materials were respectively subjected to 3 different treatments, including: ddH2O, 200 mM NaCl, and 10 mM GSH + NaCl. Compared with the ddH2O treatment, the 200 mM NaCl treatment all led to a decrease in seed germination rate, and the elongation of radicles at the seedling emergence stage was inhibited, showing significant differences; compared with the 200 mM NaCl treatment, after pretreating cotton seeds with 10 mM GSH, the seed germination rate was significantly increased, and the growth length of radicles was significantly increased, indicating that treating seeds with 10 mM GSH can significantly alleviate the inhibitory effect of NaCl stress on radicle growth and enhance the salt tolerance of cotton seedlings.

[0175] The present invention is a method for enhancing the salt tolerance of cotton seedlings by pretreating cotton seeds with reduced glutathione (GSH), providing a reference for promoting the growth of cotton in saline-alkali soil and increasing the yield of cotton in saline-alkali land.

[0176] Table 3 Germination rates of 8 cotton materials under different treatments

[0177]

[0178]

[0179] Table 4 Radicle lengths of 8 cotton materials under different treatments

[0180]

[0181] Example 3 Preliminary screening of feasible concentrations of GSH

[0182] Experiment 1:

[0183] By referring to relevant experiments and literature, the GSH concentrations were respectively set as: 0 mg / L (CK), 15 mg / L, 25 mg / L, 35 mg / L, 50 mg / L.

[0184] Reagent preparation:

[0185] GSH is reduced glutathione, with the CAS number 70-18-8.

[0186] Prepare the GSH stock solution: Weigh 1.53665 g of GSH and make up the volume to 50 ml with ddH2O to obtain the GSH stock solution.

[0187] Experimental treatment: Under the stress of 200 mM NaCl (200 mM NaCl solution), add GSH (GSH stock solution) respectively to make the final concentrations of GSH be 0 mg / L (CK), 15 mg / L, 25 mg / L, 35 mg / L, and 50 mg / L.

[0188] Operation method: Adopt the vertical double-layer filter paper method (cut the filter paper to the size of A4 paper, lay it flat on a plastic tray, moisten the filter paper with ddH2O, place the seeds on the filter paper, arrange them in a "one" shape along the long side of the filter paper, with the seeds 2 cm away from the edge of the filter paper and the growth points of the seeds facing downwards, with a 2 cm interval between the seeds horizontally, roll up the filter paper along the position of the seeds, and the seeds should be rolled in the middle of the filter paper to make the filter paper into a straight tube shape, and place the straight tube-shaped filter paper vertically in a beaker), and place the seeds in beakers containing 200 mM NaCl solutions with different GSH concentrations (0 mg / L, 15 mg / L, 25 mg / L, 35 mg / L, 50 mg / L) respectively. Incubate in an incubator at 28 °C in the dark and let stand for 3 days, and then measure the germination rate of cotton seeds and the growth of radicles under different treatments.

[0189] Germination rate = (Number of germinated seeds ÷ Total number of seeds) × 100%.

[0190] Radicle length: After the seeds germinate, the white part is regarded as the radicle length.

[0191] Experimental results: Only 15 mM GSH has a mitigating effect on the stress of 200 mM NaCl. When the GSH concentration ≥ 25 mg / L, it will inhibit the germination of cotton seeds.

[0192] Experiment 2:

[0193] In order to screen the appropriate GSH concentration, adjust it in combination with the results of Experiment 1. Set the GSH concentrations to: 0 mg / L (CK), 1 mg / L, 5 mg / L, 10 mg / L, 20 mg / L, 30 mg / L.

[0194] Reagent preparation:

[0195] GSH is reduced glutathione, with the CAS number 70-18-8.

[0196] Preparation of GSH stock solution: Weigh 1.53665 g of GSH and make up the volume to 50 ml with ddH₂O to obtain the GSH stock solution.

[0197] 200 mM NaCl solution: Weigh 11.688 g of NaCl and make up the volume to 1000 ml with ddH₂O to make the final concentration of NaCl 200 mM.

[0198] Experimental treatment: Under the stress of 200 mM NaCl (200 mM NaCl solution), add GSH (GSH stock solution) respectively to make the final concentrations of GSH 0 mg / L (CK), 1 mg / L, 5 mg / L, 10 mg / L, 20 mg / L, and 30 mg / L.

[0199] Operation method: Adopt the vertical double-layer filter paper method (cut the filter paper to the size of A4 paper, lay it flat on a plastic tray, moisten the filter paper with ddH₂O, place the seeds on the filter paper, arrange them in a "one" shape along the long side of the filter paper, with the seeds 2 cm away from the edge of the filter paper and the growth points of the seeds facing downwards, with a 2-cm interval between the seeds left and right, roll up the filter paper horizontally along the position of the seeds, and the seeds should be rolled in the middle of the filter paper so that the filter paper forms a straight tube shape, and place the straight-tube-shaped filter paper vertically in a beaker), and place the seeds in beakers containing 200 mM NaCl solution with different GSH concentrations (0 mg / L, 1 mg / L, 5 mg / L, 10 mg / L, 20 mg / L, 30 mg / L) respectively. Incubate in an incubator at 28 °C in the dark and let stand for 3 days, and then measure the germination rate of cotton seeds and the growth of radicles under different treatments (take pictures for record and calculate the radicle length).

[0200] Germination rate = (Number of germinated seeds ÷ Total number of seeds) × 100%.

[0201] Radicle length: After the seeds germinate, the white part is regarded as the radicle length.

[0202] Experimental treatment: Under the stress of 200 mM NaCl (200 mM NaCl solution), add GSH (GSH stock solution) respectively to make the final concentrations of GSH 0 mg / L (CK), 1 mg / L, 5 mg / L, 10 mg / L, 20 mg / L, 30 mg / L. Experimental results: GSH at 1 mg / L, 5 mg / L, 10 mg / L, and 20 mg / L has a relieving effect on the stress of 200 mM NaCl, but the relieving effect is not obvious and it is easy to cause seed mildew. When the GSH concentration = 30 mg / L, it will inhibit the germination of cotton seeds.

[0203] Experiment 3:

[0204] To screen for the appropriate GSH concentration, adjustments were made in combination with the results of Experiments 1 and 2. To prevent seed mildew from affecting seed germination, the GSH concentration was reduced, and the GSH concentrations were set to: 0 mg / L (CK), 1 mg / L, 3 mg / L, 5 mg / L, 7 mg / L, and 9 mg / L.

[0205] Reagent preparation:

[0206] GSH is reduced glutathione, with a CAS number of 70 - 18 - 8.

[0207] Prepare the GSH stock solution: 1.53665 g of GSH was made up to 50 ml with ddH2O to obtain the GSH stock solution.

[0208] 200 mM NaCl solution: 11.688 g of NaCl was made up to 1000 ml with ddH2O to make the final concentration of NaCl 200 mM.

[0209] Experimental treatment: Under the stress of 200 mM NaCl (200 mM NaCl solution), GSH (GSH stock solution) was added respectively to make the final GSH concentrations 0 mg / L (CK), 1 mg / L, 3 mg / L, 5 mg / L, 7 mg / L, and 9 mg / L.

[0210] Operating method: The vertical double - layer filter paper method was used (the filter paper was cut to the size of A4 paper, laid flat on a plastic tray, moistened with ddH2O, the seeds were placed on the filter paper, arranged in a "one" shape along the long side of the filter paper, the seeds were 2 cm away from the edge of the filter paper, and the growth points of the seeds faced downwards, with a 2 - cm interval between seeds left and right. The filter paper was rolled up horizontally along the position of the seeds, and the seeds should be rolled in the middle of the filter paper so that the filter paper became a straight tube shape, and the straight - tube - shaped filter paper was placed vertically in a beaker). The seeds were placed in beakers containing 200 mM NaCl solutions with different GSH concentrations (0 mg / L, 1 mg / L, 5 mg / L, 10 mg / L, 20 mg / L, 30 mg / L) respectively. They were incubated in an incubator at 28 °C in the dark and left static for 3 days. Then, the germination rate of cotton seeds and the growth of radicles under different treatments were measured (photographed and the radicle length was calculated).

[0211] Germination rate = (number of germinated seeds ÷ total number of seeds) × 100%.

[0212] Radicle length: After seed germination, the white part was regarded as the radicle length.

[0213] Experimental treatment: Under 200 mM NaCl stress, GSH was added respectively to make its final concentrations 0 mg / L (CK), 1 mg / L, 3 mg / L, 5 mg / L, 7 mg / L, and 9 mg / L. Experimental results: GSH at concentrations of 1 mg / L, 3 mg / L, 5 mg / L, 7 mg / L, and 9 mg / L had a mitigating effect on the stress of 200 mM NaCl. Although the mitigating effect was not significant, the mitigating trend was that as the GSH concentration increased, the mitigating effect enhanced.

[0214] Experiment 4:

[0215] To screen for the appropriate GSH concentration, adjustments were made in combination with the results of Experiments 1, 2, and 3. Treating the seeds with GSH and 200 mM NaCl simultaneously was changed to: first pretreating the seeds with different concentrations of GSH, that is, soaking the seeds with different concentrations of GSH first, and then subjecting the pretreated seeds to 200 mM NaCl stress. After multiple experiments, it was found that the pretreated seeds were more powerful in coping with salt stress and could better promote germination and radicle growth.

[0216] After multiple repeated experiments, the GSH concentrations were set as follows: 1 mM GSH group, 5 mM GSH group, 10 mM GSH group, 15 mM GSH group, 20 mM GSH group for screening. Finally, 10 mM GSH was determined.

[0217] The above has detailed the present invention. For those skilled in the art, without departing from the purpose and scope of the present invention and without the need for unnecessary experiments, the present invention can be implemented within a relatively wide range under equivalent parameters, concentrations, and conditions. Although specific embodiments of the present invention are given, it should be understood that the present invention can be further improved. In short, according to the principle of the present invention, this application intends to include any changes, uses, or improvements to the present invention, including those that depart from the scope disclosed in this application and are made using conventional techniques known in the art. Some basic features can be applied according to the scope of the appended claims below.

Claims

1. A plant growth regulating composition, characterized in that, The composition comprises glutathione or its salt, or their solvates.

2. A method for improving the salt tolerance of cotton plants, characterized in that, The method comprises the step of applying glutathione or its salt, or their solvates or the plant growth regulating composition according to claim 1 to a Gossypium plant during the cultivation of the Gossypium plant.

3. A cultivation method of a cotton plant, characterized in that, The method comprises the step of applying glutathione or its salt, or their solvates or the plant growth regulating composition according to claim 1 to a Gossypium plant.

4. The method according to claim 2 or 3, characterized in that, The application concentration of the glutathione is 1 mM - 20 mM; and / or, the application time of the glutathione is 1 - 24 hours.

5. The method according to any one of claims 2-4, characterized in that, The Gossypium plant to which the glutathione or its salt, or their solvates or the plant growth regulating composition is applied has higher salt tolerance than the Gossypium plant to which the glutathione or its salt, or their solvates or the plant growth regulating composition is not applied.

6. The method according to any one of claims 2-5, characterized in that, The salt tolerance is seed salt tolerance or seedling stage salt tolerance.

7. Use of glutathione or its salt, or their solvates or the plant growth regulating composition according to claim 1 in improving the salt tolerance of a Gossypium plant.

8. The use according to claim 7, characterized in that, The use comprises improving the germination rate and / or the radicle length.

9. The method according to any one of claims 2-6 or the use according to claim 7 or 8, characterized in that The Gossypium plant is selected from Gossypium herbaceum, Gossypium arboreum, Gossypium anomalum, Gossypium triphyllum, Gossypium davidsonii, Gossypium sturtianum, Gossypium australe, Gossypium bickii, Gossypium populifolium, Gossypium thurberi, Gossypium laxum, Gossypium stocksii, Gossypium somalense, Gossypium incanum, Gossypium longicalyx, Gossypium bickii, Gossypium barbadense, Gossypium hirsutum, Gossypium hawaiiense, Gossypium mustelinum, Gossypium darwinii, Gossypium lanceolatum, or any combination thereof.

10. The method and use according to claim 9, characterized in that, The Gossypium plant is selected from Zhong 9807, Zhong S9612, Jimian 27, CCRI 23, Han 109, GK50, Lumianyan 21, Xinluzao 66, or any combination thereof.

Images