Formula of low-sensitivity cereal-free soft food for removing tear stains of dogs and preparation method of low-sensitivity cereal-free soft food

Abstract

The invention relates to the technical field of tear stain removal of dogs, in particular to a low-sensitivity cereal-free soft food formula for removing tear stains of dogs and a preparation method of the low-sensitivity cereal-free soft food formula, and solves the problems that in the prior art, a tear stain removal mode generally has unstable effects, takes effect slowly and the like, the phenomenon of periorbital pigmentation is difficult to effectively improve, and the effect is poor. And some products can inhibit the problem of tear stain color deepening in a short time. The invention discloses a low-sensitivity cereal-free soft food formula for removing tear stains of dogs and a preparation method of the low-sensitivity cereal-free soft food formula. Comprising 40% of frozen duck breast, 20% of frozen chicken breast, 5% of frozen duck liver, 5% of frozen duck heart, 3% of chicken oil, 2.3% of potato powder, 2% of pea fiber, 2% of deep sea fish oil, 1% of safflower oil, 1% of linseed oil, pea protein isolate, 1% of pear powder, carrot granules, green vegetable stems, cabbage granules, spinach powder, broccoli powder and mulberry leaf powder. The traditional Chinese medicine composition has remarkable effects in the aspects of improving tear properties, reducing pigmentation, improving intestinal and immune health and the like.

Description

Technical Field

[0001] The present invention relates to the technical field of removing tear marks for dogs, and in particular to a formula of a low-allergy grain-free soft food for removing tear marks for dogs and a preparation method thereof. Background Art

[0002] Canine tear stains are brown-red stains formed around the dog's eyes due to abnormal tear secretion or poor excretion, which accumulates in the corners of the eyes for a long time. The main components are porphyrins in tears, which gradually change color under the action of air oxidation and adhere to the surface of hair and skin. Not only does it affect the dog's appearance, it may also cause local bacterial growth, irritant dermatitis and other health problems. To address this phenomenon, canine tear stains are usually improved through dietary adjustments, local cleaning and care, and drug intervention. Among them, the scientificity and safety of the diet formula have a decisive influence on the formation of tear stains. Especially in daily nutritional intake, a reasonable low-allergy and grain-free formula can effectively reduce the dog's allergic reactions and reduce the burden on the tear glands, thereby fundamentally alleviating the tear stain problem.

[0003] However, in actual use, existing tear stain removal products and technologies for dogs have gradually exposed a series of significant limitations and technical problems. Specifically, existing tear stain removal methods generally suffer from unstable and slow results, making it difficult to effectively improve periocular pigmentation. While some products can inhibit the darkening of tear stains in the short term, they cannot reduce the porphyrin content and viscosity of tears at the source, causing tears to remain easily retained and oxidatively discolored. Furthermore, many dog foods on the market still contain common allergens such as gluten and corn. Long-term consumption can easily induce digestive discomfort, itchy skin, and even chronic inflammation in dogs, further exacerbating increased eye secretions and worsening tear stain formation. More seriously, these problems not only significantly increase the cost of dog ownership and the care burden on owners, but also pose potential risks to the dog's overall health. For example, frequent eye irritation may cause corneal damage and affect vision development, which is particularly harmful to puppies and long-haired breeds. Therefore, in response to the many shortcomings of existing technologies, there is an urgent need for an innovative, hypoallergenic, grain-free soft food formula for dog tear stain removal and its preparation method to address these challenges. Summary of the Invention

[0004] The purpose of the present invention is to provide a low-allergy grain-free soft food formula for removing tear marks for dogs and a preparation method thereof, which solves the problems of unstable effect and slow effect in the existing tear mark removal methods, and is difficult to effectively improve the pigmentation phenomenon around the eyes. Although some products can inhibit the darkening of tear marks in the short term, they cannot reduce the porphyrin content and viscosity in tears from the source, resulting in tears still being easily retained and oxidized and discolored; in addition, it is easy to induce digestive system discomfort, skin itching and even chronic inflammation in dogs, further aggravating the increase in eye secretions and the problem of tear mark formation.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] A low-allergy, grain-free, soft food formula for dogs that removes tear marks and a preparation method thereof includes feed raw materials and feed additives. The feed raw material formula is characterized in that the feed raw material formula is composed of 18 ingredients, and the proportions of the components are as follows:

[0007] The formula components of the feed raw materials are: 40% frozen duck breast, 20% frozen chicken breast, 5% frozen duck liver, 5% frozen duck heart, 3% chicken fat, 2.3% potato flour, 2% pea fiber, 2% deep-sea fish oil, 1% safflower oil, 1% linseed oil, pea protein isolate, 1% pear powder, carrot grains, green vegetable stems, cabbage grains, spinach powder, broccoli powder, and mulberry leaf powder.

[0008] Preferably, the formula of the feed additive consists of thirty-one materials, which are as follows:

[0009] The formula components of the feed additive are: kelp extract 100mg / 100g, rosemary extract 50000μg / 100g, cassia seed extract 30000μg / 100g, yucca extract 20000μg / 100g, chrysanthemum extract 20000μg / 100g, tea polyphenols 10000μg / 100g, sorbitol, glycerol, calcium hydrogen phosphate, vitamin E, complex phosphate, sodium chloride, citric acid, vitamin A acetate, vitamin D3, vitamin B1, vitamin B2, vitamin B6, vitamin B12, niacin, calcium pantothenate, D-biotin, folic acid, choline chloride, ferrous sulfate, copper sulfate, manganese sulfate, zinc sulfate, calcium iodate, sodium selenite, and 2×10^9 / 100g of fire-extinguishing Bacillus coagulans.

[0010] Preferably, the feed raw material preparation method comprises the following steps:

[0011] Step 1: Raw material preparation: Prepare the required feed ingredients, including frozen duck breast, frozen chicken breast, frozen duck liver, frozen duck heart, chicken fat, potato flour, pea fiber, deep-sea fish oil, safflower oil, linseed oil, pea protein isolate, pear powder, carrot granules, green vegetable stems, cabbage granules, spinach powder, broccoli powder, and mulberry leaf powder. All raw materials are quality-checked to ensure they are free of deterioration, odor, and impurities, and meet food safety standards.

[0012] Step 2: Raw material pretreatment, meat processing: remove the frozen duck breast, frozen chicken breast, frozen duck liver, and frozen duck heart from the frozen state, place them in a thawing room or thaw them under running water until the surface is slightly soft but the center still maintains a certain hardness to facilitate subsequent cutting. Cut the thawed meat into small pieces of uniform size for subsequent processing and mixing; vegetable processing: rinse the carrot pieces, green vegetable stems, and cabbage pieces with clean water to remove surface dirt and impurities. For larger vegetable pieces, cut them into smaller pieces as appropriate to ensure that their size matches that of other raw materials so that they can be evenly distributed in the final product;

[0013] Step 3: Mix the raw materials. Put the processed meat into a large mixer and turn on the mixer to mix it thoroughly. This step is to ensure that all kinds of meat can be evenly distributed in the subsequent processing process and provide balanced nutrition for dogs. Add potato flour, pea fiber, pea protein isolate, pear powder, carrot grains, green vegetable stems, cabbage grains, spinach powder, broccoli powder, mulberry leaf powder and other plant raw materials to the mixer in turn. When adding each raw material, the mixing time should be appropriately extended to ensure that the newly added raw materials can be fully integrated with the previous mixture. Finally, slowly add chicken oil, deep-sea fish oil, safflower oil and flaxseed oil to the mixer, stirring while adding so that the oil can be evenly wrapped on the surface of other raw materials to increase the palatability and nutritional value of the product. During the mixing process, pay attention to the state of the mixture to ensure that all raw materials are fully mixed and there is no lumps.

[0014] Step 4: Forming and maturation: The mixed raw materials are formed into a soft grain forming machine. According to the product design requirements, the parameters of the forming machine are adjusted to form the soft grain into a suitable shape and size, such as granules or small pieces. The formed soft grain is placed in the cooking equipment for maturation.

[0015] Step 5: Cooling and packaging. Take the cooked soft grains out of the cooking equipment and place them on a well-ventilated cooling rack to cool naturally to room temperature. During the cooling process, be careful to avoid contamination of the soft grains. After the soft grains are completely cooled, package them. Use packaging materials with good sealing properties, such as aluminum foil bags or plastic composite bags, and divide the soft grains into specified weights and seal them to prevent them from getting damp, deteriorating, and oxidizing.

[0016] Preferably, the method for preparing the feed additive comprises the following steps:

[0017] Step 1: Prepare the required 31 feed additive raw materials, including kelp extract, rosemary extract, cassia seed extract, yucca extract, chrysanthemum extract, tea polyphenols, sorbitol, glycerol, calcium hydrogen phosphate, vitamin E, complex phosphate, sodium chloride, citric acid, vitamin A acetate, vitamin D3, vitamin B1, vitamin B2, vitamin B6, vitamin B12, niacin, calcium pantothenate, D-biotin, folic acid, choline chloride, ferrous sulfate, copper sulfate, manganese sulfate, zinc sulfate, calcium iodate, sodium selenite, and Bacillus coagulans. Conduct quality inspections on all additive raw materials to ensure that their purity, activity, and quality meet the formula requirements and are within the validity period.

[0018] Step 2: Weigh the raw materials. Use a precise electronic balance to accurately weigh each raw material according to the amount of each additive specified in the formula. For kelp extract, weigh the weight corresponding to 100mg / 100g of the formula, for rosemary extract, weigh the weight corresponding to 50,000μg / 100g of the formula, and so on. During the weighing process, be careful to avoid cross-contamination between different raw materials. Clean the balance and work surface promptly after weighing each raw material.

[0019] Step 3: Mixing: Place the weighed various additive raw materials into a small mixing container in turn. For some raw materials that are easily affected by moisture or deteriorate, ensure that their packaging is intact before adding them to the mixing container to avoid the introduction of moisture or impurities. Use a stirring rod or small mixing equipment to fully stir and mix the additive raw materials. During the mixing process, check the state of the mixture regularly to ensure that there is no lumps or unevenness;

[0020] Step 4: Storage: Seal the mixed feed additives and store them in a dry, cool, well-ventilated special storage container. Avoid direct sunlight and high temperature environment to prevent the additive from losing activity or deteriorating. Attach a label to the storage container with the name of the additive, production date, shelf life, formula number and other information for easy management and use.

[0021] Preferably, the method for mixing feed raw materials and feed additives includes the following steps:

[0022] Step 1: Proportion calculation: According to the overall formula requirements of the product, accurately calculate the amount of feed additives to be added, and ensure that the ratio of additives to soft food is in line with the formula design to achieve the expected effects of removing tear marks, hypoallergenic and gluten-free;

[0023] Step 2: Mixing: Place the prepared soft grain into a large mixing device, turn on the mixing device, and slowly stir the soft grain. Slowly and evenly sprinkle the weighed feed additives into the soft grain, continuing to stir while adding to ensure that the additives are evenly distributed in the soft grain and that each soft grain contains the appropriate amount of additive ingredients.

[0024] Step 3: Finished product inspection: Randomly sample a certain number of samples from the mixed product for sensory testing to observe whether the product's appearance, color, and smell meet quality standards, ensuring that there are no abnormalities such as odor, mildew, and impurities. Nutrient testing is also conducted to test whether the content of nutrients such as protein, fat, vitamins, and minerals in the product meets the formula requirements to ensure that the product can provide comprehensive and balanced nutrition for dogs. Microbiological testing is also conducted to test whether microbial indicators such as total bacteria count, coliform group, and salmonella in the product meet food safety standards to ensure product hygiene and safety.

[0025] Step 4: Finished product packaging and storage. The qualified finished products shall be packaged according to the specified packaging specifications. The packaging materials shall have good sealing and moisture-proof properties to maintain the quality of the product. The packaged finished products shall be stored in a dry, cool, well-ventilated warehouse, avoiding direct sunlight and high temperature environment. They shall be managed according to the first-in-first-out principle to ensure the stable quality of the product during the shelf life.

[0026] Preferably, kelp extract is rich in active ingredients such as alginate, polysaccharides, and flavonoids; rosemary extract has rosmanol, carnosol, and carnosic acid as core components; cassia seed extract contains anthraquinone components and flavonoid compounds; yucca extract contains saponins, polysaccharides, and polyphenols; and chrysanthemum extract has chrysanthemum flavonoids as core components.

[0027] The present invention has at least the following beneficial effects:

[0028] The present invention achieves effective intervention in the problem of tear marks in dogs by combining a variety of high-quality animal proteins and plant fibers in scientific proportions, combined with a variety of functional additives, on the basis of comprehensive nutrition. First, the formula uses high-digestibility animal proteins such as frozen duck breast, frozen chicken breast, frozen duck liver, and frozen duck heart as the main raw materials to provide a high-quality protein source, avoiding the use of common allergens such as gluten and corn, and fundamentally reducing the increase in eye secretions and skin inflammation caused by food allergies in dogs, thereby effectively alleviating the problem of aggravated tear marks caused by allergies; secondly, natural plant ingredients such as pea fiber, pear powder, spinach powder, broccoli powder, and mulberry leaf powder are added, which are rich in dietary fiber and antioxidants, while promoting intestinal health, enhancing the body's metabolic capacity, and helping to regulate toxin levels in the body. , reduce the deposition of porphyrins in the eyes, and improve the phenomenon of periocular pigmentation from the endogenous level; secondly, the formula specially adds a variety of plant active ingredients such as kelp extract, rosemary extract, cassia seed extract, yucca extract, chrysanthemum extract, etc., which are rich in alginic acid, polysaccharides, flavonoids, anthraquinones, saponins and other functional substances, which have good antioxidant, anti-inflammatory and choleretic effects, can effectively reduce tear viscosity, promote normal tear excretion, reduce the retention time of tears in the corners of the eyes, thereby inhibiting its oxidative discoloration process; in addition, deep-sea fish oil, safflower oil, linseed oil, etc. are rich in ω -3, ω-6 unsaturated fatty acids can improve the skin barrier function of dogs, reduce abnormal sebum secretion around the eyes, and further reduce the risk of tear stain formation; at the same time, the formula also contains probiotics such as Bacillus coagulans, which help regulate the balance of intestinal microecology, improve nutrient absorption efficiency, and indirectly affect eye health through the "gut-eye" axis mechanism, thereby strengthening the overall immune system function; in terms of preparation method, through the meticulous pretreatment of raw materials, staged mixing, precise weighing and uniform addition of feed additives, the consistency and stability of each batch of products are ensured, and the effective ingredients are fully retained and evenly distributed; the final product undergoes rigorous sensory, nutritional and microbiological testing to ensure that its safety, palatability and functionality meet the requirements of high-standard pet food; in summary, the present invention not only effectively solves the problems of unstable effect, slow effect and easy allergy of existing canine tear stain removal products, but also achieves substantial breakthroughs in improving tear properties, reducing pigmentation, and promoting intestinal and immune health. It can better meet the comprehensive needs of modern pet breeding for functionality, safety and nutritional balance, and provide a scientific, safe and efficient tear stain removal solution for dogs, especially long-haired dogs. DETAILED DESCRIPTION

[0029] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0030] Example 1

[0031] The invention comprises feed raw materials and feed additives, wherein the formula of the feed raw materials is composed of eighteen materials, and the proportions of the components are as follows:

[0032] The formula components of the feed raw materials are: 40% frozen duck breast, 20% frozen chicken breast, 5% frozen duck liver, 5% frozen duck heart, 3% chicken fat, 2.3% potato flour, 2% pea fiber, 2% deep-sea fish oil, 1% safflower oil, 1% linseed oil, pea protein isolate, 1% pear powder, carrot grains, green vegetable stems, cabbage grains, spinach powder, broccoli powder, and mulberry leaf powder.

[0033] Example 2

[0034] The formula of feed additive consists of 31 materials, which are as follows:

[0035] The formula components of the feed additive are: kelp extract 100mg / 100g, rosemary extract 50000μg / 100g, cassia seed extract 30000μg / 100g, yucca extract 20000μg / 100g, chrysanthemum extract 20000μg / 100g, tea polyphenols 10000μg / 100g, sorbitol, glycerol, calcium hydrogen phosphate, vitamin E, complex phosphate, sodium chloride, citric acid, vitamin A acetate, vitamin D3, vitamin B1, vitamin B2, vitamin B6, vitamin B12, niacin, calcium pantothenate, D-biotin, folic acid, choline chloride, ferrous sulfate, copper sulfate, manganese sulfate, zinc sulfate, calcium iodate, sodium selenite, and 2×10^9 / 100g of fire-extinguishing Bacillus coagulans.

[0036] Example 3

[0037] The feed raw material preparation method includes the following steps:

[0038] Step 1: Raw material preparation: Prepare the required feed ingredients, including frozen duck breast, frozen chicken breast, frozen duck liver, frozen duck heart, chicken fat, potato flour, pea fiber, deep-sea fish oil, safflower oil, linseed oil, pea protein isolate, pear powder, carrot granules, green vegetable stems, cabbage granules, spinach powder, broccoli powder, and mulberry leaf powder. All raw materials are quality-checked to ensure they are free of deterioration, odor, and impurities, and meet food safety standards.

[0039] Step 2: Raw material pretreatment, meat processing: remove the frozen duck breast, frozen chicken breast, frozen duck liver, and frozen duck heart from the frozen state, place them in a thawing room or thaw them under running water until the surface is slightly soft but the center still maintains a certain hardness to facilitate subsequent cutting. Cut the thawed meat into small pieces of uniform size for subsequent processing and mixing; vegetable processing: rinse the carrot pieces, green vegetable stems, and cabbage pieces with clean water to remove surface dirt and impurities. For larger vegetable pieces, cut them into smaller pieces as appropriate to ensure that their size matches that of other raw materials so that they can be evenly distributed in the final product;

[0040] Step 3: Mix the raw materials. Put the processed meat into a large mixer and turn on the mixer to mix it thoroughly. This step is to ensure that all kinds of meat can be evenly distributed in the subsequent processing process and provide balanced nutrition for dogs. Add potato flour, pea fiber, pea protein isolate, pear powder, carrot grains, green vegetable stems, cabbage grains, spinach powder, broccoli powder, mulberry leaf powder and other plant raw materials to the mixer in turn. When adding each raw material, the mixing time should be appropriately extended to ensure that the newly added raw materials can be fully integrated with the previous mixture. Finally, slowly add chicken oil, deep-sea fish oil, safflower oil and flaxseed oil to the mixer, stirring while adding so that the oil can be evenly wrapped on the surface of other raw materials to increase the palatability and nutritional value of the product. During the mixing process, pay attention to the state of the mixture to ensure that all raw materials are fully mixed and there is no lumps.

[0041] Step 4: Forming and maturation: The mixed raw materials are formed into a soft grain forming machine. According to the product design requirements, the parameters of the forming machine are adjusted to form the soft grain into a suitable shape and size, such as granules or small pieces. The formed soft grain is placed in the cooking equipment for maturation.

[0042] Step 5: Cooling and packaging. Take the cooked soft grains out of the cooking equipment and place them on a well-ventilated cooling rack to cool naturally to room temperature. During the cooling process, be careful to avoid contamination of the soft grains. After the soft grains are completely cooled, package them. Use packaging materials with good sealing properties, such as aluminum foil bags or plastic composite bags, and divide the soft grains into specified weights and seal them to prevent them from getting damp, deteriorating, and oxidizing.

[0043] Example 4

[0044] The preparation method of feed additives includes the following steps:

[0045] Step 1: Prepare the required 31 feed additive raw materials, including kelp extract, rosemary extract, cassia seed extract, yucca extract, chrysanthemum extract, tea polyphenols, sorbitol, glycerol, calcium hydrogen phosphate, vitamin E, complex phosphate, sodium chloride, citric acid, vitamin A acetate, vitamin D3, vitamin B1, vitamin B2, vitamin B6, vitamin B12, niacin, calcium pantothenate, D-biotin, folic acid, choline chloride, ferrous sulfate, copper sulfate, manganese sulfate, zinc sulfate, calcium iodate, sodium selenite, and Bacillus coagulans. Conduct quality inspections on all additive raw materials to ensure that their purity, activity, and quality meet the formula requirements and are within the validity period.

[0046] Step 2: Weigh the raw materials. Use a precise electronic balance to accurately weigh each raw material according to the amount of each additive specified in the formula. For kelp extract, weigh the weight corresponding to 100mg / 100g of the formula, for rosemary extract, weigh the weight corresponding to 50,000μg / 100g of the formula, and so on. During the weighing process, be careful to avoid cross-contamination between different raw materials. Clean the balance and work surface promptly after weighing each raw material.

[0047] Step 3: Mixing: Place the weighed various additive raw materials into a small mixing container in turn. For some raw materials that are easily affected by moisture or deteriorate, ensure that their packaging is intact before adding them to the mixing container to avoid the introduction of moisture or impurities. Use a stirring rod or small mixing equipment to fully stir and mix the additive raw materials. During the mixing process, check the state of the mixture regularly to ensure that there is no lumps or unevenness;

[0048] Step 4: Storage: Seal the mixed feed additives and store them in a dry, cool, well-ventilated special storage container. Avoid direct sunlight and high temperature environment to prevent the additive from losing activity or deteriorating. Attach a label to the storage container with the name of the additive, production date, shelf life, formula number and other information for easy management and use.

[0049] Example 5

[0050] The mixing method of feed raw materials and feed additives includes the following steps:

[0051] Step 1: Proportion calculation: According to the overall formula requirements of the product, accurately calculate the amount of feed additives to be added, and ensure that the ratio of additives to soft food is in line with the formula design to achieve the expected effects of removing tear marks, hypoallergenic and gluten-free;

[0052] Step 2: Mixing: Place the prepared soft grain into a large mixing device, turn on the mixing device, and slowly stir the soft grain. Slowly and evenly sprinkle the weighed feed additives into the soft grain, continuing to stir while adding to ensure that the additives are evenly distributed in the soft grain and that each soft grain contains the appropriate amount of additive ingredients.

[0053] Step 3: Finished product inspection: Randomly sample a certain number of samples from the mixed product for sensory testing to observe whether the product's appearance, color, and smell meet quality standards, ensuring that there are no abnormalities such as odor, mildew, and impurities. Nutrient testing is also conducted to test whether the content of nutrients such as protein, fat, vitamins, and minerals in the product meets the formula requirements to ensure that the product can provide comprehensive and balanced nutrition for dogs. Microbiological testing is also conducted to test whether microbial indicators such as total bacteria count, coliform group, and salmonella in the product meet food safety standards to ensure product hygiene and safety.

[0054] Step 4: Finished product packaging and storage. The qualified finished products shall be packaged according to the specified packaging specifications. The packaging materials shall have good sealing and moisture-proof properties to maintain the quality of the product. The packaged finished products shall be stored in a dry, cool, well-ventilated warehouse, avoiding direct sunlight and high temperature environment. They shall be managed according to the first-in-first-out principle to ensure the stable quality of the product during the shelf life.

[0055] Example 6

[0056] Kelp extract is rich in active ingredients such as alginate, polysaccharides, and flavonoids. Rosemary extract has rosmanol, carnosol, and carnosic acid as its core ingredients. Cassia seed extract contains anthraquinones and flavonoids. Yucca extract contains saponins, polysaccharides, and polyphenols. Chrysanthemum extract has chrysanthemum flavonoids as its core ingredient.

[0057] Experimental Design:

[0058] The experimental period was 30 days. During the experimental period, 24 adult pet dogs weighing 4.44±0.62kg were selected, with random sex, and divided into 3 groups with 8 replicates in each group. One group (experimental group) was fed with a simple grain-free, complete, baked soft food formula for adult dogs with tear stains, one group (competitor group) was fed with a commercially available ordinary tear stain removal food, and the other group (control group) was fed with ordinary food. Each dog was raised in a single pen in a pet dog cage (1.6m×0.8m×0.8m). Necessary immunization and deworming treatment were carried out before the experiment. The dog litter was changed every day, the pens were cleaned, and the pet pens were kept clean. During the experimental period, feeding started at 8:30 every morning, and each dog was fed 200g of basic food and 500ml of water every day. During the experimental period, the eating conditions and mental state of all pets were observed every day to check whether there were any adverse reactions such as aversion, diarrhea, and vomiting.

[0059] For feeding performance measurement, fasting dogs were weighed before the experiment and on day 30. The food intake of the test dogs was recorded before each morning feeding, and the average daily food intake of all test dogs was calculated after the experiment.

[0060] Intestinal health assessment, feces scoring Before feeding each day, score the appearance of each pet dog's feces according to Table 1.

[0061] Table 1 Stool scoring criteria

[0062]

[0063]

[0064] Blood index measurement:

[0065] After the experimental period, blood was collected from the limb veins of each animal by the animal hospital and injected into a special blood collection tube. The white blood cell count (WBC), lymphocyte count, monocyte count (MON#), neutrophil count (GRAN#), lymphocyte percentage (LYMPH%), monocyte percentage (MON%), neutrophil percentage (GRAN%), red blood cell count (RBC), hemoglobin (HGB), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin content (MCH), mean corpuscular hemoglobin concentration (MCHC), red blood cell distribution width variation coefficient (RDW) were measured. Blood routine indicators such as platelet count (PLT), mean platelet volume (MPV), platelet distribution width (PDW), platelet hematocrit (PCT), and eosinophil percentage (EOS%) were measured, as well as blood glucose (GLU), creatinine (CREA), urea (BUN), urea-to-creatinine ratio (BUN / CREA), total protein (TP), albumin (ALB), globulin (GLOB), albumin-to-globulin ratio (ALB / GLOB), alanine aminotransferase (ALT), and alkaline phosphatase (ALKP). Blood antioxidant indicators such as superoxide dismutase (SOD), total antioxidant capacity (T-AOC), glutathione peroxidase (GSH-Px), catalase (CAT), and malondialdehyde (MDA) were also measured. Blood immunoglobulins IgA, IgM, IgD, and IgG, as well as inflammatory factors such as interleukins IL-2, IL-6, IL-8, tumor necrosis factor TNF-a, and iron porphyrin were also measured.

[0066] Measurement of tear stain length: After the test, for dogs with obvious tear stains, take facial photos and measure the tear stain length. The same operator uses a high-definition digital camera (fixed focal length, natural light source) to take photos of the dog's face. The shooting angles are front and 45° on the left and right sides. The tear stain length is measured using image analysis software, starting from the inner canthus of the eye and ending at the farthest end of the tear stain, accurate to 0.1 cm.

[0067] Test results: Regarding the influence of the special formula for tear stain care of Jianli full-price grain-free adult dog baked soft food on the food intake of pet dogs, as can be seen from Table 2, when there is no significant difference in the initial weight (P>0.05), there is no significant difference in the weight and average daily food intake of the test dogs during the test period (P>0.05), and there is a tendency for a significant increase in the average daily food intake of the control group (0.05<P<0.1).

[0068] Table 2 Influence of the special formula for tear stain care of Jianli full-price grain-free adult dog baked soft food on the feeding performance of pet dogs

[0069]

[0070] Regarding the influence of the special formula for tear stain care of Jianli full-price grain-free adult dog baked soft food on the fecal score of pet dogs, as can be seen from Table 3, there is no significant difference in the fecal scores of each group of pet dogs (P>0.05).

[0071] Table 3 Influence of the special formula for tear stain care of Jianli full-price grain-free adult dog baked soft food on the fecal score of pet dogs

[0072]

[0073] Regarding the influence of the special formula for tear stain care of Jianli full-price grain-free adult dog baked soft food on the blood indexes of pet dogs

[0074] As can be seen from Table 4 and Table 5, the percentage content of basophils in the blood of the test group and the control group is significantly lower than that of the competing product group (P<0.05), and there is a tendency for a significant increase in the standard deviation of red blood cell distribution width in the blood of the test group (0. The indexes of albumin, urea nitrogen, and blood glucose in the blood of the test group are significantly lower than those of the competing product group and the control group (P<0.05), and there is a tendency for a significant decrease in the creatinine urea nitrogen in the blood of the test group (0.05<P<0.1). There is no significant difference in other blood routine and blood biochemical indexes of pet dogs (P>0.05).

[0075] As can be seen from Table 6 and Table 7, the IgG content in the blood of the test group is significantly lower than that of the control group (P<0.05), and the IL-2 content in the blood of the test group is significantly lower than that of the competing product group and the control group (P<0.05); the SOD index in the blood of the test group is significantly lower than that of the competing product group and the control group (P<0.05); the T-AOC index in the blood of the test group is significantly higher than that of the competing product group and the control group (P<0.05); there is no significant difference in other blood immunity and antioxidant indexes of pet dogs (P>0.05).

[0076] As shown in Table 8, there was no significant difference in the blood iron porphyrin content among the pet dogs in each group (P>0.05).

[0077] Table 4 Effects of Simple Grain Whole Grain Free Adult Dog Baked Soft Food Tear Stain Care Formula on Routine Blood Parameters in Pet Dogs

[0078]

[0079]

[0080] Table 5 Effects of Simple Grain Whole Grain Free Adult Dog Baked Soft Food Tear Stain Care Formula on Blood Immune Parameters in Pet Dogs

[0081]

[0082]

[0083] Table 5 Effects of Simple Grain Whole Grain Free Adult Dog Baked Soft Food Tear Stain Care Formula on Blood Immune Parameters in Pet Dogs

[0084]

[0085] Table 7 Effects of Simple Grain Whole Grain Free Adult Dog Baked Soft Food Tear Stain Care Formula on Blood Antioxidant Indexes in Pet Dogs

[0086]

[0087] Table 8 Effects of Simple Grain Whole Grain Free Adult Dog Baked Soft Food Tear Stain Care Formula on Hemoglobin Active Center Indicators in Pet Dogs

[0088]

[0089] The effect of Jianli whole-price grain-free baked soft food for adult dogs on tear stain removal in dogs is shown in Table 9. The tear stain length in the experimental group is the shortest, while the tear stain length in the competitor group is the longest.

[0090] Table 9 Effect of Simple Grain Whole Grain Free Adult Dog Baked Soft Food Tear Stain Care Formula on the Length of Pet Dogs

[0091]

[0092] This project studies the effects of the Simply Grain complete grain-free adult dog baked soft food tear stain care formula on the health benefits of tear stains in pet dogs. The following conclusions were drawn from the experiment of feeding Simply Grain complete grain-free adult dog baked soft food tear stain care formula to pet dogs:

[0093] 1) Feeding the Simple Grain Whole Grain Free Adult Dog Baked Soft Food Tear Stain Care Formula will not adversely affect the dog's weight, food intake, and water intake.

[0094] 2) Fecal scores of dogs fed the Simple Grain Whole Grain-Free Adult Dog Baked Soft Food Tear Stain Care Formula were within normal range;

[0095] 3) Feeding the Simple Grain Whole Price Grain-Free Adult Dog Baked Soft Food Tear Stain Care Formula will have an impact on the blood indicators of pet dogs. The percentage of blood basophils in the experimental group decreased, and the food can reduce the body's allergic or chronic inflammatory response; the standard deviation of the red blood cell distribution width in the experimental group showed a significant upward trend; the blood albumin, urea nitrogen and blood sugar indicators in the experimental group decreased.

[0096] The moderate protein content of the food can reduce the excretion pressure on the pet dog's kidneys; the blood creatinine and urea nitrogen in the experimental group showed a significant downward trend;

[0097] 4) Feeding the Simple Grain Whole Grain-Free Adult Dog Baked Soft Food Tear Stain Care Formula affects pet dogs' blood immune indicators. The experimental group showed a significant increase in immunoglobulin G (IgG) and a decrease in interleukin-2 (IL-2), which can reduce pet dogs' sensitivity to food allergies or chronic antigen stimulation.

[0098] 5) Feeding the Simple Grain Whole-Price Grain-Free Adult Dog Baked Soft Food Tear Stain Care Formula affected the antioxidant indicators of pet dogs. The superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) in the experimental group were significantly increased, which can reduce the oxidative reaction of pet dogs.

[0099] 6) Dogs fed with the Simple Grain Whole Price Grain-Free Adult Dog Baked Soft Food Tear Stain Care Formula had shorter tear stains, improved periorbital pigmentation, lowered tear viscosity, and had cleaner hair around their eyes.

[0100] The above shows and describes the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions merely illustrate the principles of the present invention. Various changes and modifications may be made to the present invention without departing from the spirit and scope of the present invention. Such changes and modifications are intended to fall within the scope of the present invention. The scope of protection claimed by the present invention is defined by the appended claims and their equivalents.

Claims

1. A low-allergy, grain-free soft food formula for dogs to remove tear marks, comprising feed raw materials and feed additives, characterized in that: The formula of the feed raw material consists of eighteen kinds of materials, and the proportions of the components are as follows: The feed raw materials are formulated as follows: 40% frozen duck breast, 20% frozen chicken breast, 5% frozen duck liver, 5% frozen duck heart, 3% chicken fat, 2.3% potato flour, 2% pea fiber, 2% deep-sea fish oil, 1% safflower oil, 1% linseed oil, pea protein isolate, 1% pear powder, carrot pieces, green vegetable stems, cabbage pieces, spinach powder, broccoli powder, and mulberry leaf powder.

2. A low-allergy, grain-free soft food formula for removing tear marks for dogs according to claim 1, characterized in that: The formula of the feed additive consists of thirty-one materials, which are as follows: The feed additive comprises the following ingredients: 100 mg / 100 g of kelp extract, 50,000 μg / 100 g of rosemary extract, 30,000 μg / 100 g of cassia seed extract, 20,000 μg / 100 g of yucca extract, 20,000 μg / 100 g of chrysanthemum extract, 10,000 μg / 100 g of tea polyphenols, sorbitol, glycerol, calcium hydrogen phosphate, vitamin E, complex phosphate, sodium chloride, citric acid, vitamin A acetate, vitamin D3, vitamin B1, vitamin B2, vitamin B6, vitamin B12, niacin, calcium pantothenate, D-biotin, folic acid, choline chloride, ferrous sulfate, copper sulfate, manganese sulfate, zinc sulfate, calcium iodate, sodium selenite, and 2×10^9 fire-extinguishing Bacillus coagulans per 100 g.

3. A method for preparing a low-allergy, grain-free soft food formula for dogs to remove tear marks, characterized in that: The feed raw material preparation method comprises the following steps Step 1: Raw material preparation: Prepare the required feed ingredients, including frozen duck breast, frozen chicken breast, frozen duck liver, frozen duck heart, chicken fat, potato flour, pea fiber, deep-sea fish oil, safflower oil, linseed oil, pea protein isolate, pear powder, carrot granules, green vegetable stems, cabbage granules, spinach powder, broccoli powder, and mulberry leaf powder. All raw materials are quality-checked to ensure they are free of deterioration, odor, and impurities, and meet food safety standards. Step 2: Raw material pretreatment, meat processing: remove the frozen duck breast, frozen chicken breast, frozen duck liver, and frozen duck heart from the frozen state, place them in a thawing room or thaw them under running water until the surface is slightly soft but the center still maintains a certain hardness to facilitate subsequent cutting. Cut the thawed meat into small pieces of uniform size for subsequent processing and mixing; vegetable processing: rinse the carrot pieces, green vegetable stems, and cabbage pieces with clean water to remove surface dirt and impurities. For larger vegetable pieces, cut them into smaller pieces as appropriate to ensure that their size matches that of other raw materials so that they can be evenly distributed in the final product; Step 3: Mix the raw materials. Put the processed meat into a large mixer and turn on the mixer to mix it thoroughly. This step is to ensure that all kinds of meat can be evenly distributed in the subsequent processing process and provide balanced nutrition for dogs. Add potato flour, pea fiber, pea protein isolate, pear powder, carrot grains, green vegetable stems, cabbage grains, spinach powder, broccoli powder, mulberry leaf powder and other plant raw materials to the mixer in turn. When adding each raw material, the mixing time should be appropriately extended to ensure that the newly added raw materials can be fully integrated with the previous mixture. Finally, slowly add chicken oil, deep-sea fish oil, safflower oil and flaxseed oil to the mixer, stirring while adding so that the oil can be evenly wrapped on the surface of other raw materials to increase the palatability and nutritional value of the product. During the mixing process, pay attention to the state of the mixture to ensure that all raw materials are fully mixed and there is no lumps. Step 4: Forming and maturation: The mixed raw materials are formed into a soft grain forming machine. According to the product design requirements, the parameters of the forming machine are adjusted to form the soft grain into a suitable shape and size, such as granules or small pieces. The formed soft grain is placed in the cooking equipment for maturation. Step 5: Cooling and packaging. Take the cooked soft grains out of the cooking equipment and place them on a well-ventilated cooling rack to cool naturally to room temperature. During the cooling process, be careful to avoid contamination of the soft grains. After the soft grains are completely cooled, package them. Use packaging materials with good sealing properties, such as aluminum foil bags or plastic composite bags, and divide the soft grains into specified weights and seal them to prevent them from getting damp, deteriorating, and oxidizing.

4. The method for preparing a low-allergy, grain-free soft food formula for removing tear marks for dogs according to claim 3, characterized in that: The feed additive preparation method comprises the following steps: Step 1: Prepare the required 31 feed additive raw materials, including kelp extract, rosemary extract, cassia seed extract, yucca extract, chrysanthemum extract, tea polyphenols, sorbitol, glycerol, calcium hydrogen phosphate, vitamin E, complex phosphate, sodium chloride, citric acid, vitamin A acetate, vitamin D3, vitamin B1, vitamin B2, vitamin B6, vitamin B12, niacin, calcium pantothenate, D-biotin, folic acid, choline chloride, ferrous sulfate, copper sulfate, manganese sulfate, zinc sulfate, calcium iodate, sodium selenite, and Bacillus coagulans. Conduct quality inspections on all additive raw materials to ensure that their purity, activity, and quality meet the formula requirements and are within the validity period. Step 2: Weigh the raw materials. Use a precise electronic balance to accurately weigh each raw material according to the amount of each additive specified in the formula. For kelp extract, weigh the weight corresponding to 100mg / 100g of the formula, for rosemary extract, weigh the weight corresponding to 50,000μg / 100g of the formula, and so on. During the weighing process, be careful to avoid cross-contamination between different raw materials. Clean the balance and work surface promptly after weighing each raw material. Step 3: Mixing: Place the weighed various additive raw materials into a small mixing container in turn. For some raw materials that are easily affected by moisture or deteriorate, ensure that their packaging is intact before adding them to the mixing container to avoid the introduction of moisture or impurities. Use a stirring rod or small mixing equipment to fully stir and mix the additive raw materials. During the mixing process, check the state of the mixture regularly to ensure that there is no lumps or unevenness; Step 4: Storage: Seal the mixed feed additives and store them in a dry, cool, well-ventilated special storage container. Avoid direct sunlight and high temperature environment to prevent the additive from losing activity or deteriorating. Attach a label to the storage container with the name of the additive, production date, shelf life, formula number and other information for easy management and use.

5. The method for preparing a low-allergy, grain-free soft food formula for removing tear marks for dogs according to claim 4, characterized in that: The mixing method of the feed raw materials and feed additives comprises the following steps: Step 1: Proportion calculation: According to the overall formula requirements of the product, accurately calculate the amount of feed additives to be added, and ensure that the ratio of additives to soft food is in line with the formula design to achieve the expected effects of removing tear marks, hypoallergenic and gluten-free; Step 2: Mixing: Place the prepared soft grain into a large mixing device, turn on the mixing device, and slowly stir the soft grain. Slowly and evenly sprinkle the weighed feed additives into the soft grain, continuing to stir while adding to ensure that the additives are evenly distributed in the soft grain and that each soft grain contains the appropriate amount of additive ingredients. Step 3: Finished product inspection: Randomly sample a certain number of samples from the mixed product for sensory testing to observe whether the product's appearance, color, and smell meet quality standards, ensuring that there are no abnormalities such as odor, mildew, and impurities. Nutrient testing is also conducted to test whether the content of nutrients such as protein, fat, vitamins, and minerals in the product meets the formula requirements to ensure that the product can provide comprehensive and balanced nutrition for dogs. Microbiological testing is also conducted to test whether microbial indicators such as total bacteria count, coliform group, and salmonella in the product meet food safety standards to ensure product hygiene and safety. Step 4: Finished product packaging and storage. The qualified finished products shall be packaged according to the specified packaging specifications. The packaging materials shall have good sealing and moisture-proof properties to maintain the quality of the product. The packaged finished products shall be stored in a dry, cool, well-ventilated warehouse, avoiding direct sunlight and high temperature environment. They shall be managed according to the first-in-first-out principle to ensure the stable quality of the product during the shelf life.

6. The low-allergy, grain-free soft food formula for removing tear marks for dogs according to claim 1, characterized in that: The kelp extract is rich in active ingredients such as alginate, polysaccharides, and flavonoids; the rosemary extract has rosmanol, carnosol, and carnosic acid as core components; the cassia seed extract contains anthraquinone components and flavonoid compounds; the yucca extract contains saponins, polysaccharides, and polyphenols; and the chrysanthemum extract has chrysanthemum flavonoids as core components.

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