Fermentation decoction for improving growth and immune functions of broiler chickens and preparation method thereof

Abstract

The invention belongs to the technical field of animal health care, and particularly relates to a fermented decoction for improving growth and immune functions of broiler chickens and a preparation method thereof. The fermentation decoction for improving the growth and immune functions of the broiler chickens is prepared by fermenting the following raw materials in parts by weight: 8-12 parts of codonopsis pilosula, 3-7 parts of angelica sinensis, 8-12 parts of dogwood, 3-7 parts of cinnamon and 3-7 parts of portulaca oleracea. The fermentation decoction can significantly increase the weight of broiler chickens, significantly increase thymus indexes and spleen indexes, increase the content of IgG and IgM in serum, and improve the immune function of broiler chickens while promoting the growth of broiler chickens. The preparation method of the fermentation decoction is simple, and is suitable for industrial production and market popularization and application.

Description

Technical Field

[0001] The invention belongs to the technical field of animal health care, and particularly relates to a fermentation decoction for improving the growth and immune function of broiler chickens and a preparation method thereof. Background Art

[0002] Traditional Chinese medicine fermentation technology utilizes enzymes and microorganisms, under suitable culture conditions, to complete the fermentation process by catalyzing the decomposition of processed drugs under the action of fungi and enzymes. The fermentation process can alter the medicinal properties of traditional Chinese medicines, enhancing or developing new efficacy, expanding their scope of use and meeting clinical needs. As a traditional processing method for traditional Chinese medicines, fermentation yields unique chemical compositions and pharmacological effects.

[0003] In the practice of meat duck breeding, the application of fermented Chinese medicine not only effectively promotes the healthy growth of meat ducks, but also plays an important role in improving their immune function and enhancing meat quality.

[0004] Fermentation strains are the foundation of traditional Chinese medicine fermentation and the key to its success. Different microorganisms metabolize and utilize different substances. Selecting the appropriate fermentation strain for different traditional Chinese medicines to maximize their utilization requires extensive experimental research.

[0005] Therefore, how to provide a fermented product that is simple to prepare and easy to promote and apply to improve the growth and immune function of broilers is a technical problem that needs to be solved urgently by those skilled in the art. Summary of the Invention

[0006] The present invention aims to address the deficiencies of the prior art by providing a fermented decoction and a method for preparing the same for improving the growth and immune function of broiler chickens. The fermented decoction significantly increases broiler chicken weight, thymus index, and spleen index, and increases serum IgG and IgM levels, thereby promoting growth and improving immune function in broiler chickens.

[0007] In order to achieve the above object, the present invention provides the following technical solutions:

[0008] The first object of the present invention is to provide a fermentation decoction for improving the growth and immune function of broiler chickens.

[0009] The second object of the present invention is to provide a method for preparing the fermentation decoction for improving the growth and immune function of broiler chickens.

[0010] The third object of the present invention is to provide the use of the fermented decoction for improving the growth and immune function of broiler chickens.

[0011] To achieve the first purpose, the technical solution adopted by the present invention is:

[0012] The invention provides a fermented decoction for improving the growth and immune function of broiler chickens. The fermented decoction is prepared by fermenting the following Chinese medicinal raw materials in parts by weight: 8-12 parts of codonopsis pilosula, 3-7 parts of angelica sinensis, 8-12 parts of cornus officinalis, 3-7 parts of cinnamon bark and 3-7 parts of purslane.

[0013] Furthermore, the fermented decoction is prepared by fermenting the following Chinese medicinal raw materials in parts by weight: 10 parts of Codonopsis pilosula, 5 parts of Angelica sinensis, 10 parts of Cornus officinalis, 5 parts of cinnamon bark and 5 parts of Portulaca oleracea.

[0014] Furthermore, the fermented decoction is prepared by fermenting the following Chinese medicinal raw materials in parts by weight: 8 parts of Codonopsis pilosula, 7 parts of Angelica sinensis, 8 parts of Cornus officinalis, 7 parts of cinnamon bark and 3 parts of Portulaca oleracea.

[0015] To achieve the second purpose, the technical solution adopted by the present invention is:

[0016] A method for preparing a fermented decoction for improving the growth and immune function of broiler chickens comprises the following steps:

[0017] (1) Wash and dry Codonopsis pilosula, Chinese angelica root, Cornus officinalis, Cinnamon bark, and Portulaca oleracea, grind through an 80-mesh sieve, sterilize at 121°C for 30 minutes, let cool, and set aside;

[0018] (2) The composite bacterial agent was inoculated into MRS solid medium for activation culture, and then inoculated into MRS liquid medium to make a colony count of 10 9 CFU / ml bacterial suspension;

[0019] (3) adding the bacterial suspension of step (2) to the mixture of step (1) at a weight ratio of bacterial suspension to mixture of 0.05-0.07:10-15, mixing, sealing, and static fermentation at 25-35° C. for 72 h to 96 h;

[0020] (4) After fermentation is complete, the fermentation liquid is centrifuged and filtered with activated carbon to obtain the supernatant.

[0021] Furthermore, the composite bacterial agent consists of Trichoderma, Bacillus subtilis, Lactobacillus plantarum and Pediococcus acidilactici, wherein the weight ratio of Trichoderma, Bacillus subtilis, Lactobacillus plantarum and Pediococcus acidilactici is 1-3:1-3:2-4:2-4.

[0022] Furthermore, the weight ratio of Trichoderma, Bacillus subtilis, Lactobacillus plantarum and Pediococcus acidilactici in the composite bacterial agent is 1:2:3:3.

[0023] Furthermore, in step (3), the weight ratio of the bacterial suspension to the mixture is 0.06:10.

[0024] Furthermore, in step (3), the mixture and the bacterial suspension are fermented at 30° C. for 92 hours.

[0025] To achieve the third purpose, the technical solution adopted by the present invention is:

[0026] The present invention provides use of the fermented decoction for improving the growth and immune function of broiler chickens in preparing a medicine for preventing or improving the growth and immune function of broiler chickens.

[0027] The effects of the Chinese medicinal raw materials of the present invention are as follows:

[0028] Codonopsis: sweet in taste, neutral in nature, enters the lung and spleen meridians, tonifies the middle and replenishes qi, quenches thirst, strengthens the spleen and benefits the lungs, nourishes blood and produces body fluids; used for spleen and lung qi deficiency, poor appetite and fatigue, cough and shortness of breath, qi and blood deficiency, sallow complexion, palpitations and shortness of breath, thirst due to loss of body fluids, internal heat and thirst.

[0029] Angelica: spicy and bitter in taste, warm in nature, enters the heart, liver, and spleen meridians, replenishes blood and promotes blood circulation, regulates menstruation and relieves pain, moisturizes and lubricates the intestines; used for irregular menstruation, amenorrhea, dysmenorrhea, accumulation of masses, metrorrhagia, abdominal pain due to deficiency and cold, paralysis, numbness of the skin, dry intestines and constipation, heaviness after dysentery, carbuncles, ulcers, and injuries from falls.

[0030] Cornus officinalis: sour and astringent in taste, slightly warm in nature, enters the liver and kidney meridians, nourishes the liver and kidneys, astringes the essence and strengthens the body; used for dizziness and tinnitus, soreness of the waist and knees, impotence and spermatorrhea, enuresis and frequent urination, metrorrhagia and leukorrhea, profuse sweating and collapse.

[0031] Cinnamon: spicy and sweet in taste, very hot in nature, enters the kidney, spleen, and heart meridians, replenishes fire and yang, dispels cold and relieves pain, and promotes blood circulation and menstruation.

[0032] Ma Chi Mi: sour in taste, cold in nature, clears away heat and detoxifies, cools blood and stops dysentery, removes dampness and relieves stranguria, clears away heat and detoxifies, removes dampness and stops leukorrhea; used for damp-heat in the lower part of the body, leucorrhea with yellow color, sticky and smelly taste, short and yellow urine, thirst and bitter taste in the mouth, red tongue with yellow coating, slippery pulse, and can also be used for diarrhea and dysentery caused by damp-heat.

[0033] After the traditional Chinese medicine of the present invention is fermented by probiotics, certain traditional Chinese medicine components are decomposed and converted into new drug components. In particular, the various enzymes produced by the probiotics can promote the full absorption and utilization of the effective components of the traditional Chinese medicine by the animal body, and the traditional Chinese medicine contains rich nutrients that can promote the large-scale proliferation of probiotics. The two complement each other and better exert the pharmacological effects of the traditional Chinese medicine. The traditional Chinese medicine fermented by the composite probiotics has both the effects of traditional Chinese medicine and the probiotic auxiliary functions of microorganisms.

[0034] Compared with the prior art, the present invention has the following beneficial effects:

[0035] The fermented decoction of the present invention fully exerts the efficacy of probiotics, traditional Chinese medicine and fermentation products thereof, can significantly promote the growth of broiler chickens, and improve the immune function of broiler chickens.

[0036] The fermentation decoction of the present invention significantly increases the body weight of broiler chickens, significantly improves the thymus index and spleen index, and increases the IgG and IgM contents in serum. While promoting the growth of broiler chickens, the immune function of the broiler chickens is improved. The preparation method of the fermentation decoction of the present invention is simple and is suitable for industrial production and market promotion and application. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] Figure 1 Comparison of body weight among groups

[0038] Compared with the control group, * P<0.05, ** P<0.01; compared with the Example 1 group, # P<0.05, ## P<0.01.

[0039] Figure 2 Comparison of average daily weight gain among groups

[0040] Compared with the control group, * P<0.05, ** P<0.01; compared with the Example 1 group, # P<0.05, ## P<0.01.

[0041] Figure 3 Comparison of spleen index among groups

[0042] Compared with the control group, * P<0.05, ** P<0.01; compared with the Example 1 group, # P<0.05, ## P<0.01.

[0043] Figure 4 Comparison of bursal index among groups.

[0044] Figure 5 Comparison of thymus index among groups

[0045] Compared with the control group, * P<0.05, ** P<0.01; compared with the Example 1 group, # P<0.05.

[0046] Figure 6 Comparison of IgG content in each group

[0047] Compared with the control group, * P<0.05, ** P<0.01; compared with the Example 1 group, # P<0.05, ## P<0.01.

[0048] Figure 7 Comparison of IgM content in each group

[0049] Compared with the control group, * P<0.05, ** P<0.01; compared with the Example 1 group, # P<0.05.

[0050] Figure 8 Comparison of IgA levels in each group. DETAILED DESCRIPTION

[0051] The embodiments of the present invention will be described in detail below with reference to examples. However, those skilled in the art will understand that the following examples are only used to illustrate the present invention and should not be construed as limiting the scope of the present invention.

[0052] Example 1

[0053] A fermented decoction for preventing or improving the growth and immune function of broiler chickens is prepared by fermenting the following ingredients: 10g of Codonopsis pilosula, 5g of Chinese angelica, 10g of Cornus officinalis, 5g of cinnamon bark, and 5g of Portulaca oleracea.

[0054] The preparation method is:

[0055] (1) Wash and dry Codonopsis pilosula, Chinese angelica root, Cornus officinalis, Cinnamon bark, and Portulaca oleracea, grind through an 80-mesh sieve, sterilize at 121°C for 30 minutes, let cool, and set aside;

[0056] (2) The composite bacterial agent was inoculated into MRS solid medium for activation culture, and then inoculated into MRS liquid medium to make a colony count of 10 9 CFU / ml bacterial suspension;

[0057] (3) adding the bacterial suspension of step (2) to the mixture of step (1) at a weight ratio of bacterial suspension to mixture of 0.06:10, mixing, sealing, and fermenting at 30°C for 92 hours;

[0058] (4) After fermentation is complete, the fermentation liquid is centrifuged and filtered with activated carbon to obtain the supernatant.

[0059] The weight ratio of Trichoderma, Bacillus subtilis, Lactobacillus plantarum and Pediococcus acidilactici in the composite bacterial agent is 1:2:3:3.

[0060] Example 2

[0061] A fermented decoction for preventing or improving the growth and immune function of broiler chickens is prepared by fermenting the following ingredients: 8g of Codonopsis pilosula, 7g of Chinese angelica, 8g of Cornus officinalis, 7g of cinnamon bark, and 3g of Portulaca oleracea.

[0062] The preparation method is:

[0063] (1) Wash and dry Codonopsis pilosula, Chinese angelica root, Cornus officinalis, Cinnamon bark, and Portulaca oleracea, grind through an 80-mesh sieve, sterilize at 121°C for 30 minutes, let cool, and set aside;

[0064] (2) The composite bacterial agent was inoculated into MRS solid medium for activation culture, and then inoculated into MRS liquid medium to make a colony count of 10 9 CFU / ml bacterial suspension;

[0065] (3) adding the bacterial suspension of step (2) to the mixture of step (1) at a weight ratio of bacterial suspension to mixture of 0.07:10, mixing, sealing, and fermenting at 25° C. for 96 h;

[0066] (4) After fermentation is complete, the fermentation liquid is centrifuged and filtered with activated carbon to obtain the supernatant.

[0067] The weight ratio of Trichoderma, Bacillus subtilis, Lactobacillus plantarum and Pediococcus acidilactici in the composite bacterial agent is 1:1:4:4.

[0068] Example 3

[0069] A fermented decoction for preventing or improving the growth and immune function of broiler chickens is prepared by fermenting the following ingredients: 12g of Codonopsis pilosula, 3g of Chinese angelica, 12g of Cornus officinalis, 3g of cinnamon bark, and 7g of Portulaca oleracea.

[0070] The preparation method is:

[0071] (1) Wash and dry Codonopsis pilosula, Chinese angelica root, Cornus officinalis, Cinnamon bark, and Portulaca oleracea, grind through an 80-mesh sieve, sterilize at 121°C for 30 minutes, let cool, and set aside;

[0072] (2) The composite bacterial agent was inoculated into MRS solid medium for activation culture, and then inoculated into MRS liquid medium to make a colony count of 10 9 CFU / ml bacterial suspension;

[0073] (3) adding the bacterial suspension of step (2) to the mixture of step (1) at a weight ratio of bacterial suspension to mixture of 0.05:15, mixing, sealing, and static fermentation at 35°C for 72 hours;

[0074] (4) After fermentation is complete, the fermentation liquid is centrifuged and filtered with activated carbon to obtain the supernatant.

[0075] The weight ratio of Trichoderma, Bacillus subtilis, Lactobacillus plantarum and Pediococcus acidilactici in the composite bacterial agent is 3:1:2:4.

[0076] Example 4

[0077] A fermented decoction for preventing or improving the growth and immune function of broiler chickens is prepared by fermenting the following ingredients: 10g of Codonopsis pilosula, 7g of Chinese angelica, 8g of Cornus officinalis, 5g of cinnamon bark, and 7g of Portulaca oleracea.

[0078] The preparation method is:

[0079] (1) Wash and dry Codonopsis pilosula, Chinese angelica root, Cornus officinalis, Cinnamon bark, and Portulaca oleracea, grind through an 80-mesh sieve, sterilize at 121°C for 30 minutes, let cool, and set aside;

[0080] (2) The composite bacterial agent was inoculated into MRS solid medium for activation culture, and then inoculated into MRS liquid medium to make a colony count of 10 9 CFU / ml bacterial suspension;

[0081] (3) adding the bacterial suspension of step (2) to the mixture of step (1) at a weight ratio of bacterial suspension to mixture of 0.07:15, mixing, sealing, and fermenting at 30° C. for 72 h;

[0082] (4) After fermentation is complete, the fermentation liquid is centrifuged and filtered with activated carbon to obtain the supernatant.

[0083] The weight ratio of Trichoderma, Bacillus subtilis, Lactobacillus plantarum and Pediococcus acidilactici in the composite bacterial agent is 1:3:2:2.

[0084] Example 5

[0085] A fermented decoction for preventing or improving the growth and immune function of broiler chickens is prepared by fermenting the following ingredients: 12g of Codonopsis pilosula, 5g of Chinese angelica, 10g of Cornus officinalis, 7g of cinnamon bark, and 3g of Portulaca oleracea.

[0086] The preparation method is:

[0087] (1) Wash and dry Codonopsis pilosula, Chinese angelica root, Cornus officinalis, Cinnamon bark, and Portulaca oleracea, grind through an 80-mesh sieve, sterilize at 121°C for 30 minutes, let cool, and set aside;

[0088] (2) The composite bacterial agent was inoculated into MRS solid medium for activation culture, and then inoculated into MRS liquid medium to make a colony count of 10 9 CFU / ml bacterial suspension;

[0089] (3) adding the bacterial suspension of step (2) to the mixture of step (1) at a weight ratio of bacterial suspension to mixture of 0.05:10, mixing, sealing, and fermenting at 35° C. for 72 h;

[0090] (4) After fermentation is complete, the fermentation liquid is centrifuged and filtered with activated carbon to obtain the supernatant.

[0091] The weight ratio of Trichoderma, Bacillus subtilis, Lactobacillus plantarum and Pediococcus acidilactici in the composite bacterial agent is 2:3:3:2.

[0092] Comparative Example 1

[0093] A fermented decoction for preventing or improving the growth and immune function of broiler chickens is prepared by fermenting the following ingredients: 10g of angelica sinensis, 15g of cornus officinalis, 5g of cinnamon bark and 5g of purslane.

[0094] Compared with Example 1, the difference is that Codonopsis pilosula is not contained, and the amount of Codonopsis pilosula is increased to the amount of Angelica sinensis and Cornus officinalis. The preparation method is similar to Example 1.

[0095] Comparative Example 2

[0096] A fermented decoction for preventing or improving the growth and immune function of broiler chickens is prepared by fermenting the following ingredients: 12g of Codonopsis pilosula, 13g of Cornus officinalis, 5g of Cinnamon bark and 5g of Purslane.

[0097] Compared with Example 1, the difference is that the Chinese angelica is not contained, and the amount of Chinese angelica is increased to the amount of Codonopsis pilosula and Cornus officinalis. The preparation method is similar to that of Example 1.

[0098] Comparative Example 3

[0099] A fermented decoction for preventing or improving the growth and immune function of broiler chickens is prepared by fermenting the following raw materials: 10g of Codonopsis pilosula, 10g of Chinese angelica, 10g of cinnamon bark and 5g of Purslane.

[0100] Compared with Example 1, the difference is that Cornus officinalis is not contained, and the amount of Cornus officinalis is increased to the amount of Angelica sinensis and Cinnamon bark.

[0101] Comparative Example 4

[0102] A fermented decoction for preventing or improving the growth and immune function of broiler chickens is prepared by fermenting the following ingredients: 10g of Codonopsis pilosula, 5g of Chinese angelica, 10g of Cornus officinalis, 5g of cinnamon bark, and 5g of Portulaca oleracea.

[0103] Compared with Example 1, the difference lies in that the fermentation agent is different. The composite bacterial agent consists of Lactobacillus plantarum and Pediococcus acidilactici in a weight ratio of 3:3, and the weight ratio of the bacterial suspension to the mixture is the same.

[0104] Comparative Example 5

[0105] A fermented decoction for preventing or improving the growth and immune function of broiler chickens is prepared by fermenting the following ingredients: 10g of Codonopsis pilosula, 5g of Chinese angelica, 10g of Cornus officinalis, 5g of cinnamon bark, and 5g of Portulaca oleracea.

[0106] Compared with Example 1, the difference lies in that the fermentation agent is different. The composite bacterial agent consists of Bacillus subtilis, Lactobacillus plantarum and Pediococcus acidilactici in a weight ratio of 3:3:3, and the weight ratio of the bacterial suspension to the mixture is the same.

[0107] Comparative Example 6

[0108] A fermented decoction for preventing or improving the growth and immune function of broiler chickens is prepared by fermenting the following ingredients: 10g of Codonopsis pilosula, 5g of Chinese angelica, 10g of Cornus officinalis, 5g of cinnamon bark, and 5g of Portulaca oleracea.

[0109] Compared with Example 1, the difference lies in that the fermentation agent is different. The composite bacterial agent consists of Trichoderma, Lactobacillus plantarum and Pediococcus acidilactici in a weight ratio of 3:3:3, and the weight ratio of the bacterial suspension to the mixture is the same.

[0110] Experimental Example Effects of the Fermented Decoction of the Present Invention on Growth Performance and Immune Function of Broilers

[0111] 1. Experimental Methods

[0112] 900 one-day-old white-feathered broiler chickens were randomly divided into nine groups of 100 chickens each. The control group, Example 1 group, Example 2 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, Comparative Example 4 group, Comparative Example 5 group, and Comparative Example 6 group received 2.5% fermented decoction in their drinking water. The control group received no medication in its drinking water. For a 42-day experimental period, the basal diet for the white-feathered broiler chickens was formulated based on the nutritional levels for broilers specified in the NRC (2012) and was divided into two stages: 1-3 weeks of age and 4-6 weeks of age. The composition and nutritional levels of the basal diet are shown in Table 1.

[0113] Table 1 Basic feed composition and nutritional level (dry matter basis)

[0114]

[0115] The henhouses were thoroughly flushed and fumigated before the trial. The experimental chickens were housed in three cages: upper, middle, and lower. The house temperature was raised to 32-35°C 24 hours before chick placement, then lowered by 2-3°C weekly until it remained between 21-24°C. Continuous lighting and natural ventilation were used. Throughout the trial, the birds had free access to food and water, and were vaccinated according to standardized henhouse management procedures.

[0116] 2. Sample collection and indicator testing

[0117] 2.1 Growth performance

[0118] On day 42 of the experiment, the broiler chickens were fasted for 12 hours and weighed. Average daily gain (ADAG) was calculated as follows: ADAG = total weight gain / (number of experimental days × number of experimental days).

[0119] 2.2 Immune indicators

[0120] On day 42 of the experiment, 10 mL of blood was collected from the subwing vein of five broiler chickens in each group. The blood was allowed to rest for 4 hours and then centrifuged at 4000 rpm for 6 minutes to collect the supernatant serum. The broiler chickens were slaughtered, and the spleen, bursa of Fabricius, and thymus were weighed. Serum immunoglobulin A (IgA), immunoglobulin M (IgM), and immunoglobulin G (IgG) were measured. Organ index = organ weight / pre-slaughter live weight × 100%.

[0121] 3. Statistical methods

[0122] Graphpad Prism 9.0 software was used for data processing, and the measured data were expressed as mean ± standard deviation ( ) indicated that multiple groups were compared using one-way analysis of variance, and group comparisons were performed using SNK-q. P < 0.05 was considered statistically significant.

[0123] 4. Experimental Results

[0124] 4.1 Effects on broiler growth performance

[0125] Compared with the control group, Example 1 and Example 2 groups significantly increased the 42-day-old body weight of broiler chickens. The effect of increasing body weight in Example 1 group was the best, and was significantly better than that in Comparative Example 1, Comparative Example 2, Comparative Example 3, Comparative Example 4, Comparative Example 5, and Comparative Example 6 groups. Figure 1 .

[0126] Compared with the control group, Example 1 and Example 2 groups significantly increased the average daily weight gain of broiler chickens. The effect of Example 1 group on increasing the average daily weight gain was the best, and was significantly better than Comparative Example 1, Comparative Example 2, Comparative Example 3, Comparative Example 4, Comparative Example 5, and Comparative Example 6. Figure 2 .

[0127] 4.2 Effects on Immune Function

[0128] Compared with the control group, Example 1 and Example 2 groups significantly increased the thymus index and spleen index of broiler chickens. The effect of Example 1 group on increasing the thymus index and spleen index was the best, and was significantly better than Comparative Example 1, Comparative Example 2, Comparative Example 3, Comparative Example 4, Comparative Example 5, and Comparative Example 6. Figure 3 、 4 、5.

[0129] Compared with the control group, Example 1 and Example 2 groups significantly increased the IgG and IgM contents in the serum of broiler chickens. The effect of Example 1 on increasing the IgG and IgM contents in the serum of broiler chickens was the best, and was significantly better than Comparative Example 1, Comparative Example 2, Comparative Example 3, Comparative Example 4, Comparative Example 5, and Comparative Example 6. Figure 6 、 7 、8.

[0130] The fermented decoction of the present invention significantly increases the body weight of broiler chickens, significantly improves the thymus index and spleen index, and increases the IgG and IgM contents in serum, thereby improving the immune function of broiler chickens while promoting the growth of broiler chickens.

[0131] While the present invention has been described in detail above using general descriptions and specific embodiments, it will be apparent to those skilled in the art that modifications and improvements may be made to the present invention. Therefore, such modifications and improvements, without departing from the spirit of the present invention, are intended to be within the scope of the present invention.

Claims

1. A fermented decoction for improving the growth and immune function of broiler chickens, characterized in that: The fermented decoction is prepared by fermenting the following Chinese medicinal raw materials in parts by weight: 8-12 parts of Codonopsis pilosula, 3-7 parts of Angelica sinensis, 8-12 parts of Cornus officinalis, 3-7 parts of cinnamon bark and 3-7 parts of Portulaca oleracea.

2. The fermented decoction for improving broiler growth and immune function according to claim 1, characterized in that: The fermented decoction is prepared by fermenting the following Chinese medicinal raw materials in parts by weight: 10 parts of Codonopsis pilosula, 5 parts of Angelica sinensis, 10 parts of Cornus officinalis, 5 parts of cinnamon bark and 5 parts of Portulaca oleracea.

3. The fermented decoction for improving broiler growth and immune function according to claim 1, characterized in that: The fermented decoction is prepared by fermenting the following Chinese medicinal raw materials in parts by weight: 8 parts of Codonopsis pilosula, 7 parts of Angelica sinensis, 8 parts of Cornus officinalis, 7 parts of Cinnamon bark and 3 parts of Portulaca oleracea.

4. The fermented decoction for improving broiler growth and immune function according to any one of claims 1 to 3, characterized in that: The preparation method of the fermentation decoction for improving broiler growth and immune function is as follows: (1) Wash and dry Codonopsis pilosula, Chinese angelica root, Cornus officinalis, Cinnamon bark, and Portulaca oleracea, grind through an 80-mesh sieve, sterilize at 121°C for 30 minutes, let cool, and set aside; (2) The composite bacterial agent was inoculated into MRS solid medium for activation culture, and then inoculated into MRS liquid medium to make a colony count of 10 9 CFU / ml bacterial suspension; (3) adding the bacterial suspension of step (2) to the mixture of step (1) at a weight ratio of bacterial suspension to mixture of 0.05-0.07:10-15, mixing, sealing, and static fermentation at 25-35° C. for 72 h to 96 h; (4) After fermentation is complete, the fermentation liquid is centrifuged and filtered with activated carbon to obtain the supernatant.

5. The fermented decoction for improving the growth and immune function of broiler chickens according to claim 4, characterized in that: The composite bacterial agent consists of Trichoderma, Bacillus subtilis, Lactobacillus plantarum and Pediococcus acidilactici, wherein the weight ratio of Trichoderma, Bacillus subtilis, Lactobacillus plantarum and Pediococcus acidilactici is 1-3:1-3:2-4:2-4.

6. The fermented decoction for improving broiler growth and immune function according to claim 5, characterized in that: The weight ratio of Trichoderma, Bacillus subtilis, Lactobacillus plantarum and Pediococcus acidilactici in the composite bacterial agent is 1:2:3:

3.

7. The fermented decoction for improving the growth and immune function of broiler chickens according to claim 4, characterized in that: In the step (3), the weight ratio of the bacterial suspension to the mixture is 0.06:

10.

8. The fermented decoction for improving the growth and immune function of broiler chickens according to claim 7, characterized in that: In the step (3), the mixture and the bacterial suspension are fermented at 30° C. for 92 hours.

9. Use of the fermented decoction for improving broiler growth and immune function according to any one of claims 1 to 3 in the preparation of a drug for preventing or improving broiler growth and immune function.

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