Embryo transfer device and method

Abstract

The embryo transfer device comprises a clamp, a carrying rod and a sleeve, the clamp is provided with a structure used for installing the carrying rod and the sleeve, the carrying rod and the sleeve are matched with the structure in an embedded mode, the carrying rod and / or the sleeve and components on the structure generate acceleration through relative acting force, and the clamping device is used for clamping the carrying rod and / or the sleeve and the components on the structure. And the embryo on the carrying rod falls into the sleeve. The embryo transfer can be completed without a microscope and a mouth suction tube, and the embryo transfer device is convenient to use in pastures and remote environments. According to the embryo transfer device, embryo transfer can be completed without a microscope and a mouth suction tube, the transferred embryo is almost free of damage, the situation that the embryo is lost in the transfer process is avoided to the maximum extent, and the embryo transfer device further has the advantages of being simple in structure, easy to implement, easy to operate, low in cost and the like.

Description

Technical Field

[0001] The present invention relates to the field of bioengineering technology, and in particular to an embryo transfer device and method. Background Art

[0002] The use of freeze-thaw embryo technology has significant implications for the livestock industry. First, it provides an effective tool for genetic improvement in livestock. Through frozen embryo technology, the genetic characteristics of high-quality livestock can be preserved and passed down, eliminating the need for continuous live breeding. This means producers can select the best parents to enhance the quality of their offspring, thereby improving livestock production efficiency and profitability. Second, frozen embryos facilitate international trade and the dissemination of genetic resources in the livestock industry. Frozen embryos can be safely transferred between countries, promoting international livestock cooperation and the sharing of genetic resources. This helps improve breeding standards in different regions, enhances breed diversity, and reduces the risk of genetic deficiencies. Furthermore, frozen embryos help reduce the risk of animal disease transmission. Traditional live breeding methods can lead to the spread of disease, but the use of frozen embryos can reduce this risk, contributing to a healthier and more sustainable livestock industry.

[0003] Despite the remarkable success of cryopreservation technology, particularly vitrification, in human medicine and its widespread application in reproductive medicine, its application in the livestock industry has been significantly limited. This limitation is primarily due to factors such as cost, expertise, and pasture environment. First, vitrification requires expensive equipment and expertise, which may be impractical in the livestock industry. Specifically, thawed embryos need to be transferred to recipient cows as quickly as possible. Embryologists use a mouth pipette under a microscope to transfer embryos from the carrier straw to straws, a process that requires meticulous training and a specialized laboratory environment. In pasture environments, there is often a lack of personnel with these specialized skills, making the application of this technology difficult. Furthermore, pastures are often located in remote areas and lack the necessary infrastructure and equipment, such as microscopes and freezing equipment, making the application of the technology even more difficult.

[0004] Therefore, designing a device and technology for transferring embryos from straws to straws without the need for microscopes and complex equipment is of great significance for the large-scale application of freeze-thaw technology in animal husbandry. Summary of the Invention

[0005] In summary, the present invention aims to address at least one of the defects (shortcomings) of the above-mentioned prior art and to provide an embryo transfer device and method.

[0006] An embryo transfer device of the present invention comprises: a clamp, a carrier rod and a sleeve. The clamp is provided with a structure for mounting the carrier rod and the sleeve. The carrier rod and the sleeve are respectively embedded and matched with the structure. The carrier rod and / or the sleeve and components on the structure generate acceleration through relative force, causing the embryo on the carrier rod to fall into the sleeve.

[0007] The component is fixedly connected to the structure, or the component is movably connected to the structure.

[0008] The clamp includes a clamp body having a first plane and a second plane. The second plane is located between the two adjacent first planes, and the height of the second plane is lower than that of the first plane.

[0009] The structure comprises a rod mounting portion for mounting a rod and a sleeve mounting portion for mounting a sleeve.

[0010] The rod mounting portion and the sleeve mounting portion are respectively arranged on the first plane of the clamp.

[0011] The rod mounting portion is provided with one or more rod mounting grooves for mounting rods, and the sleeve mounting portion is provided with one or more sleeve mounting grooves for mounting sleeves. The rods and sleeves are respectively embedded in the rod mounting grooves and the sleeve mounting grooves.

[0012] A pair of lugs are respectively provided on the two side walls of each of the carrying rod installation groove and the sleeve installation groove to be opposite to each other and to be engaged with the carrying rod and the sleeve.

[0013] There is a gap between each of the lugs and the inner wall of the carrier rod mounting portion or the sleeve mounting portion.

[0014] The distance between the pair of lugs is less than or equal to the distance between the rod mounting slots or the sleeve mounting slots.

[0015] The carrier rod is inserted into the carrier rod installation groove, the lugs are deformed and move toward the gap, and the carrier rod and the components generate acceleration through friction, causing the embryos on the carrier rod to fall into the sleeve.

[0016] The component is one of a rack, a raised plate, and an embossed plate.

[0017] A pair of the components are respectively and oppositely arranged on one side inner wall or both sides inner walls of each of the carrying rod installation grooves.

[0018] The component is integrally formed with the carrier rod mounting portion, or the component is movably connected to the carrier rod mounting portion.

[0019] The one-piece molding processing method includes one of bonding, CNC machine tool processing, injection molding, laser engraving, etching, and 3D printing, and the movable connection includes one of elastic connection and riveting.

[0020] The carrier rod comprises a handle, a carrier rod body and a front end sheet. The front end sheet is provided with a first groove, and the first groove contains embryos and / or culture fluid.

[0021] The utility model further comprises a base for supporting the clamp, wherein the base is provided with a second groove corresponding to the shape of the clamp, and the clamp is arranged in the second groove.

[0022] It also includes an alignment device for positioning the carrier rod so that it can be easily plugged into the sleeve. The alignment device is integrally formed or plugged into the second plane.

[0023] The alignment device includes an inserting portion and a positioning portion, the inserting portion is fixedly connected to the positioning portion, and at least one carrier rod positioning hole and at least one sleeve positioning hole are respectively opened at both ends of the positioning portion, and the carrier rod positioning hole is connected to the sleeve positioning hole.

[0024] The carrier rod positioning hole and the sleeve positioning hole are trumpet-shaped and gradually expand from the inside to the outside. An opening is provided above the carrier rod positioning hole for facilitating the user to observe the insertion status.

[0025] The embryo falls off from the first groove of the thin plate at the front end of the carrier rod into the liquid or on the solid surface.

[0026] The carrier rod is placed in the sleeve, or the carrier rod is plugged into the sleeve.

[0027] There is a gap between the carrier rod and the sleeve for allowing liquid and / or embryos to pass through.

[0028] The sleeve is one of a wheat straw, a centrifuge tube and a test tube.

[0029] The relative acting force is friction force and / or elastic force generated by deformation.

[0030] The acceleration is used to produce movement of the structure housing the embryo.

[0031] The mode of generating the motion is reciprocating motion.

[0032] The acceleration is multiple accelerations.

[0033] The number of accelerations is 1-500 times.

[0034] The invention also comprises a negative pressure device for sucking the liquid in the test tube into the sleeve.

[0035] The negative pressure device is a manual negative pressure device or an automatic negative pressure device.

[0036] The manual negative pressure device is one of a syringe, a rubber-tipped straw or an oral straw, and the automatic negative pressure device is one of a syringe pump, a peristaltic pump, a plunger pump, a diaphragm pump, a vacuum pump, a centrifugal pump, an impeller pump, and a piston pump.

[0037] The negative pressure device absorbs the culture fluid in the test tube and injects it into the sleeve, so that at least one section of liquid is formed in the sleeve.

[0038] The liquid is one of a culture fluid, a transplantation fluid or an operating fluid.

[0039] The automatic negative pressure device includes a shell, a circuit board and a motor. The circuit board and the motor are installed in the shell. A control button and a display screen are provided on the surface of the shell. The motor, the control button and the display screen are electrically connected to the circuit board respectively.

[0040] The automatic negative pressure device has a liquid inlet and a liquid outlet. The liquid inlet is connected to the external test tube through a first connecting tube, and the liquid outlet is connected to the sleeve through a second connecting tube. The automatic negative pressure device absorbs the liquid in the test tube through negative pressure and injects the liquid into the sleeve.

[0041] The present invention also includes an embryo transfer method comprising the following steps:

[0042] a. Aspirate the liquid in the test tube to form at least one section of liquid in the cannula;

[0043] b. Insert the front end sheet of the carrier rod into the sleeve, so that the carrier rod and the sleeve are respectively engaged with the clamp;

[0044] c. Remove the sleeve from the fixture;

[0045] d. Aspirate the liquid in the test tube again to form at least one section of liquid in the sleeve.

[0046] The step a is preceded by the following step: inserting the cannula into the rubber stopper, and the test tube contains liquid.

[0047] The method of aspirating the liquid in the test tube in step a can be manual or automatic.

[0048] The step a is followed by the following steps: placing the syringe and the cannula on a fixture and adjusting the relative position of the liquid in the cannula.

[0049] In step b, the carrier rod and / or sleeve and the clamp generate acceleration through relative force, so that the embryo on the carrier rod falls into the sleeve.

[0050] After step d, the following step is further included: removing the sleeve from the rubber plug and placing it into a transplant gun for embryo transplantation.

[0051] The relative acting force is friction force and / or elastic force generated by deformation.

[0052] The liquid is one of a culture fluid, a transplantation fluid or an operating fluid.

[0053] By adopting the above technical solution, compared with the prior art, the beneficial effect produced by the present invention is that: the present invention provides an elastic lug on the clamp, and a rack arranged on the lug, and generates acceleration through the friction between the rack and the carrier rod, so that the embryo on the carrier rod is transferred to the sleeve. The embryo transfer can be completed without the need for a microscope and an oral pipette, which is convenient for use in pastures and remote environments.

[0054] The embryos transferred by the present invention are almost undamaged and the loss of embryos during the transfer process is avoided to the greatest extent. The present invention also has the characteristics of simple structure, easy implementation, simple operation (only simple learning is required to use it, and no embryologist is required to be present), and low cost. BRIEF DESCRIPTION OF THE DRAWINGS

[0055] Figure 1 It is a schematic diagram of the overall structure of Example 1 of the present invention.

[0056] Figure 2 It is a schematic structural diagram of the clamp of the present invention.

[0057] Figure 3 It is a top view of the clamp of the present invention.

[0058] Figure 4 This invention Figure 3 A partially enlarged schematic diagram of part I.

[0059] Figure 5 This invention Figure 3 A partial enlarged schematic diagram of part II.

[0060] Figure 6 It is a structural schematic diagram of the base of the present invention.

[0061] Figure 7 It is a structural schematic diagram of the alignment device of the present invention.

[0062] Figure 8 It is another structural schematic diagram of the alignment device of the present invention.

[0063] Figure 9 It is a structural schematic diagram of the carrier rod of the present invention.

[0064] Figure 10 It is a schematic diagram of the overall structure of Example 2 of the present invention.

[0065] Figure 11 It is a schematic diagram of the overall structure of Example 2 of the present invention.

[0066] Figure 12 It is a schematic structural diagram of the clamp of Example 2 of the present invention.

[0067] Figure 13 Schematic diagram of the connection between the automatic negative pressure device and the sleeve in Example 1 of the present invention.

[0068] Figure 14 2 is a schematic diagram of an exploded view of the automatic negative pressure device of Example 1 of the present invention.

[0069] Figure 15 It is a three-dimensional schematic diagram of the automatic negative pressure device of Example 1 of the present invention. DETAILED DESCRIPTION

[0070] The following examples are provided to further explain and supplement the present invention and do not constitute any limitation to the present invention.

[0071] The technical solution of the present invention is further described below with reference to the accompanying drawings and embodiments.

[0072] Example 1:

[0073] like Figures 1 to 12 As shown, an embryo transfer device of the present invention includes: a clamp 10, a carrier rod 20 and a sleeve 30. The clamp 10 is provided with a structure 40 for mounting the carrier rod and the sleeve. The carrier rod 20 and the sleeve 30 are respectively embedded in the structure. The carrier rod 20 and / or the sleeve 30 and the component 43 on the structure 40 generate acceleration through relative force, causing the embryo on the carrier rod 20 to fall into the sleeve 30.

[0074] Furthermore, the component 43 is fixedly connected to the structure 40 , or the component 43 is movably connected to the structure 40 .

[0075] In this embodiment, the component 43 and the structure 40 are fixedly connected.

[0076] like Figure 2 As shown, further, the clamp includes a clamp body 13, and the clamp body 13 has a first plane 14 and a second plane 15, the second plane 15 is located between the two adjacent first planes 14, and the height of the second plane 15 is lower than the height of the first plane 14.

[0077] Furthermore, the structure 40 includes a rod mounting portion 41 for mounting a rod and a sleeve mounting portion 42 for mounting a sleeve.

[0078] Furthermore, the rod mounting portion 41 and the sleeve mounting portion 42 are respectively arranged on the first plane 14 of the clamp.

[0079] like Figure 4 , Figure 5 As shown, the rod mounting portion 41 is further provided with one or more rod mounting grooves 411 for mounting rods, and the rods 20 are embedded in the rod mounting grooves 411. The sleeve mounting portion 42 is provided with one or more sleeve mounting grooves 421 for mounting sleeves, and the sleeves 30 are embedded in the sleeve mounting grooves 421.

[0080] like Figure 4 , Figure 5 As shown, further, a pair of lugs 412 and 422 are respectively provided on the two side walls of each of the carrier rod installation groove 411 and the sleeve installation groove 421 to engage with the carrier rod 20 and the sleeve 30. In this embodiment, the lugs are elastic. When the carrier rod or the sleeve is placed, the lugs are squeezed and deformed. When the carrier rod or the sleeve is taken out, the lugs return to their original shape.

[0081] like Figure 4 , Figure 5 As shown, further, a gap 413 , 423 is respectively defined between each of the lugs 412 , 422 and the inner wall of the rod mounting portion 41 or the sleeve mounting portion 42 .

[0082] Furthermore, the distance between the pair of lugs 412 and 422 is less than or equal to the distance between the rod mounting groove 411 or the sleeve mounting groove 421 .

[0083] In this embodiment, the distance between the pair of lugs 412 and 422 is smaller than the distance between the rod mounting groove 411 or the sleeve mounting groove 421 .

[0084] Furthermore, the carrier rod 20 is inserted into the carrier rod installation groove 411 , the lugs 412 and 422 are deformed and move toward each other toward the gap, and the carrier rod 20 and the components generate acceleration through friction, causing the embryo on the carrier rod 20 to fall into the sleeve 30 .

[0085] In this embodiment, the component 43 is one of a rack, a raised plate, and an embossed plate.

[0086] Preferably, the component is a rack.

[0087] like Figure 4 As shown, further, a pair of the components 43 are respectively and oppositely arranged on one side inner wall or both sides inner walls of each of the carrying rod installation grooves 411 .

[0088] Furthermore, the component 43 is integrally formed with the rod mounting portion 41 , or the component 43 is movably disposed on the rod mounting portion 41 .

[0089] Furthermore, the one-piece molding processing method includes one of bonding, CNC machine tool processing, injection molding, laser engraving, etching, and 3D printing, and the movable connection includes one of elastic connection and riveting.

[0090] like Figure 9 As shown, specifically, the carrier rod 20 includes a handle 21, a carrier rod body 22 and a front thin sheet 23. The front thin sheet has a first groove 231. The first groove 231 contains embryos and / or culture medium, and of course also includes but is not limited to biological materials such as eggs and cells. This application does not limit this.

[0091] like Figure 6 As shown, the present invention also includes a base 50 for supporting the clamp, and the base 50 is provided with a second groove 51 corresponding to the shape of the clamp 10. The clamp 10 is set in the second groove 51. The weight of the base is greater than the weight of the clamp, so when the clamp is placed in the base, the clamp can be made more stable and less likely to shake.

[0092] like Figure 2 , Figure 3 As shown, further, a third groove 151 extending in the width direction is provided on the second plane 15 .

[0093] like Figure 7 , Figure 8 As shown, the present invention further includes an alignment device 60 for positioning the carrier rod so that it can be easily plugged into the sleeve. The alignment device 60 is integrally formed with or plugged into the second plane 15.

[0094] Furthermore, the alignment device 60 is plugged into the third groove 151 .

[0095] like Figure 7 , Figure 8 As shown, specifically, the positioning device 60 includes a plug-in portion 61 and a positioning portion 62, the plug-in portion 61 is fixedly connected to the positioning portion 62, and at least one carrier rod positioning hole 621 and at least one sleeve positioning hole 622 are respectively opened at both ends of the positioning portion, and the carrier rod positioning hole 621 is connected to the sleeve positioning hole 622.

[0096] like Figure 7 Furthermore, the rod positioning hole 621 and the sleeve positioning hole 622 are trumpet-shaped and gradually expand from the inside to the outside. An opening 6211 is provided above the rod positioning hole 621 for the user to conveniently observe the insertion status.

[0097] Furthermore, the embryo falls off from the first groove 231 of the front end sheet of the carrier rod into the liquid or solid surface.

[0098] Furthermore, the carrying rod 20 is placed in the sleeve 30, or the carrying rod 20 is plugged into the sleeve 30. In this embodiment, the carrying rod 20 is plugged into the sleeve 30.

[0099] Furthermore, there is a gap between the carrier rod 20 and the sleeve 30 for liquid and / or embryos to pass through.

[0100] Furthermore, the sleeve 30 is one of a wheat straw, a centrifuge tube, and a test tube.

[0101] In this embodiment, preferably, the sleeve is a wheat straw.

[0102] Furthermore, the relative force is friction force and / or elastic force generated by deformation.

[0103] Furthermore, the acceleration is used to move a structure containing the embryo.

[0104] Furthermore, the motion is generated in a reciprocating manner.

[0105] Furthermore, the acceleration is multiple accelerations.

[0106] Furthermore, the number of accelerations is 1-500 times.

[0107] like Figures 13-15 As shown, the present invention further comprises a negative pressure device for sucking the liquid in the test tube into the sleeve.

[0108] Furthermore, the negative pressure device is a manual negative pressure device (not shown in the figure) or an automatic negative pressure device 50.

[0109] The manual negative pressure device is one of a syringe, a rubber-tipped straw or an oral straw, and the automatic negative pressure device is one of a syringe pump, a peristaltic pump, a plunger pump, a diaphragm pump, a vacuum pump, a centrifugal pump, an impeller pump, and a piston pump.

[0110] Furthermore, the negative pressure device absorbs the liquid in the test tube and injects it into the sleeve, so that at least one section of liquid is formed in the sleeve.

[0111] Specifically, the liquid is one of a culture fluid, a transplantation fluid or an operating fluid.

[0112] like Figure 13 , as shown in FIG14 , specifically, the automatic negative pressure device 50 includes a housing 51, within which a circuit board 52 and a motor 53 are mounted. A control button 54 and a display screen 55 are provided on the surface of the housing 51. The motor 53, control button 54, and display screen 55 are electrically connected to the circuit board, respectively. In this embodiment, the control button 54 is used to turn the automatic negative pressure device on and off, and the display screen 55 is used to display specific values for aspirating and injecting liquid into the cannula.

[0113] like Figure 13 Furthermore, the automatic negative pressure device 50 has a liquid inlet and a liquid outlet (not shown in the figure), the liquid inlet is connected to an external test tube (not shown in the figure) through a connecting tube (not shown in the figure), and the liquid outlet is connected to the sleeve 30 through a connecting tube 56. The automatic negative pressure device absorbs the liquid in the test tube through negative pressure and injects the liquid into the sleeve.

[0114] The present invention also includes an embryo transfer method, which uses a syringe to draw liquid from a test tube and inject the liquid into a cannula. The method specifically includes the following steps:

[0115] a. First, remove the syringe with the rubber stopper, cannula and test tube containing the culture medium;

[0116] b. Insert the cannula into the rubber stopper (it is better to cover the first section of cotton wool), and then use a syringe to draw culture medium from the test tube to form three sections of liquid in the cannula;

[0117] c. Place the syringe and cannula in the fixture and adjust the position of the third liquid;

[0118] d. Remove the automatically thawed embryos, substrate, straw support rack, and straws from the external machine, and remove the straws from the straw support rack.

[0119] e. Insert the front end sheet of the carrier rod into the sleeve, and clamp the carrier rod and sleeve together into the clamp lug;

[0120] f. Remove the carrier rod from the fixture from the lug;

[0121] g. Remove the syringe and cannula from the fixture, and use the syringe to draw culture fluid from the test tube to form five sections of liquid in the cannula, with the first section of liquid in contact with the second section of cotton wool;

[0122] Remove the cannula from the rubber stopper and place it in the transplant gun for embryo transplantation.

[0123] The present invention also includes an embryo transfer method, which uses an automatic negative pressure device to suck liquid from a test tube through negative pressure and inject the liquid into a cannula. The method includes the following steps:

[0124] a. Remove the automatic negative pressure device with 1-10 rubber stoppers, the cannula, and the test tube containing the culture medium;

[0125] Insert the cannula into the rubber stopper (it is best to cover the first section of cotton wool), turn on the automatic negative pressure device to absorb the culture medium and air in the test tube to form three sections of liquid in the cannula;

[0126] b. Place the sleeve on the fixture;

[0127] c. Remove the automatically thawed embryos, substrate, straw support rack, and straws from the external machine, and remove the straws from the straw support rack;

[0128] d. Insert the front end sheet of the carrier rod into the sleeve, and clamp the carrier rod and sleeve together into the clamp lug;

[0129] Remove the carrier rod from the clamp from the lug;

[0130] e. Remove the cannula from the fixture and use the automatic negative pressure device to draw culture fluid into the test tube to form five sections of liquid in the cannula, with the first section of liquid in contact with the second section of cotton wool;

[0131] f. Remove the cannula from the rubber stopper and place it into the transplant gun for embryo transplantation.

[0132] Example 2

[0133] Figures 10-12 This is a structural diagram of another embodiment of the present invention. The basic structure and operation steps of this embodiment are the same as those of embodiment 1. Figures 10-12 The labels in Figure 1 The same components have the same functions. For the sake of brevity, the description of these same components is omitted. The differences are as follows:

[0134] In this embodiment, if Figure 11 , a plurality of components 43 are arranged on the second plane 15 of the clamp, and each component 43 is perpendicular to the second plane 15.

[0135] Furthermore, every two components form a group and are arranged opposite to each other, and multiple groups of components are horizontally arranged along the length direction of the second plane.

[0136] Furthermore, the teeth on each group of relatively arranged components can be arranged with both sides facing inward, or both sides facing outward, or the teeth on one group of components can be arranged with both sides facing inward, and the teeth on the adjacent group of components can be arranged with both sides facing outward. The purpose of such arrangement is to ensure that the carrier rod placed in the carrier rod mounting groove is in full contact with each component to generate friction.

[0137] like Figure 12 In this embodiment, a plurality of lugs 412 and 422 are respectively provided on both side walls of the first plane 14 and the second plane 15 , and the plurality of lugs 412 and 422 are arranged opposite to each of the components.

[0138] like Figure 12In this embodiment, the alignment device 60 is disposed on the first plane 14 and located between the rod mounting portion and the sleeve mounting portion. The alignment device 60 includes a base 61 and a guide groove 62. The front end sheet 23 of the rod is disposed in the guide groove 62, and the front end sheet portion of the rod is positioned in the guide groove 62.

[0139] Although the present invention is disclosed through the above embodiments, the scope of the present invention is not limited thereto. Without departing from the concept of the present invention, the above components can be replaced with similar or equivalent elements known to those skilled in the art.

Claims

1. An embryo transfer device, characterized in that: include: A clamp (10), a carrier rod (20) and a sleeve (30); the clamp (10) is provided with a structure (40) for mounting the carrier rod and the sleeve; the carrier rod (20) and the sleeve (30) are respectively embedded and matched with the structure; the carrier rod (20) and / or the sleeve (30) and a component (43) on the structure (40) generate acceleration through relative force, so that the embryo on the carrier rod (20) falls off into the sleeve (30).

2. The embryo transfer device according to claim 1, characterized in that The component (43) is fixedly connected to the structure (40), or the component (43) is movably connected to the structure (40).

3. The embryo transfer device according to claim 1, characterized in that The clamp comprises a clamp body (13), wherein the clamp body (13) has a first plane (14) and a second plane (15), wherein the second plane (15) is located between two adjacent first planes (14), and the height of the second plane (15) is lower than the height of the first plane (14).

4. The embryo transfer device according to claim 1, characterized in that The structure (40) comprises a rod mounting portion (41) for mounting a rod and a sleeve mounting portion (42) for mounting a sleeve.

5. The embryo transfer device according to claim 4, characterized in that: The rod mounting portion (41) and the sleeve mounting portion (42) are respectively arranged on the first plane (14) of the clamp.

6. The embryo transfer device according to claim 5, characterized in that: The rod mounting portion (41) is provided with one or more rod mounting grooves (411) for mounting rods, and the sleeve mounting portion (42) is provided with one or more sleeve mounting grooves (421) for mounting sleeves. The rod (20) and sleeve (30) are respectively embedded in the rod mounting grooves (411) and the sleeve mounting grooves (421).

7. The embryo transfer device according to claim 6, characterized in that: A pair of lugs (412, 422) for engaging with the carrying rod (20) and the sleeve (30) are respectively provided on the two side walls of each of the carrying rod installation groove (411) and the sleeve installation groove (421).

8. The embryo transfer device according to claim 7, characterized in that: There is a gap (413, 423) between each lug (412, 422) and the inner wall of the rod mounting portion (41) or the sleeve mounting portion (42).

9. The embryo transfer device according to claim 8, characterized in that: The spacing between the pair of lugs (412, 422) is less than or equal to the spacing between the rod mounting groove (411) or the sleeve mounting groove (421).

10. The embryo transfer device according to claim 9, characterized in that: The carrier rod (20) is inserted into the carrier rod installation groove (411), the lugs (412, 422) are deformed and move toward each other toward the gap, and the carrier rod (20) and the component (43) generate acceleration through friction, causing the embryo on the carrier rod (20) to fall off into the sleeve (30).

11. The embryo transfer device according to claim 10, characterized in that: The component (43) is one of a rack, a raised plate, and an embossed plate.

12. The embryo transfer device according to claim 11, characterized in that: A pair of components (43) are respectively and relatively arranged on one side inner wall or both sides inner walls of each of the carrying rod installation grooves (411).

13. The embryo transfer device according to claim 12, characterized in that: The component (43) is integrally formed with the rod-carrying mounting portion (41), or the component (43) is movably arranged on the rod-carrying mounting portion (41).

14. The embryo transfer device according to claim 13, characterized in that: The one-piece molding processing method includes one of bonding, CNC machine tool processing, injection molding, laser engraving, etching, and 3D printing, and the movable connection includes one of elastic connection and riveting.

15. The embryo transfer device according to claim 10, characterized in that The carrier rod (20) comprises a handle (21), a carrier rod body (22) and a front end sheet (23). The front end sheet is provided with a first groove (231), and the first groove (231) contains embryos and / or culture fluid.

16. The embryo transfer device according to claim 1, characterized in that It also includes a base (50) for supporting the clamp, wherein the base (50) is provided with a second groove (51) corresponding to the shape of the clamp, and the clamp (10) is arranged in the second groove (51).

17. The embryo transfer device according to claim 3, characterized in that: It also includes a positioning device (60) for positioning the carrier rod so that it can be easily plugged into the sleeve. The positioning device (60) is integrally formed or plugged into the second plane (15).

18. The embryo transfer device according to claim 17, characterized in that: The alignment device (60) comprises an inserting portion (61) and a positioning portion (62), wherein the inserting portion (61) and the positioning portion (62) are fixedly connected, and at least one carrier rod positioning hole (621) and at least one sleeve positioning hole (622) are respectively provided at both ends of the positioning portion, and the carrier rod positioning hole (621) and the sleeve positioning hole (622) are communicated with each other.

19. The embryo transfer device according to claim 18, characterized in that The rod positioning hole (621) and the sleeve positioning hole (622) are trumpet-shaped and gradually expand from the inside to the outside. An opening (6211) is provided above the rod positioning hole (621) for the user to conveniently observe the insertion status.

20. The embryo transfer device according to claim 15, wherein: The embryo falls off from the first groove (231) of the thin sheet at the front end of the carrier rod into the liquid or on a solid surface.

21. The embryo transfer device according to claim 20, characterized in that: The carrier rod (20) is placed in the sleeve (30), or the carrier rod (20) is plugged into the sleeve (30).

22. The embryo transfer device according to claim 21, characterized in that There is a gap between the carrier rod (20) and the sleeve (30) for allowing liquid and / or embryos to pass through.

23. The embryo transfer device according to claim 22, characterized in that The sleeve (30) is one of a wheat straw, a centrifuge tube, and a test tube.

24. The embryo transfer device according to claim 1, wherein: The relative acting force is friction force and / or elastic force generated by deformation.

25. The embryo transfer device according to claim 10, wherein: The acceleration is used to produce movement of the structure housing the embryo.

26. The embryo transfer device according to claim 25, characterized in that The mode of generating the motion is reciprocating motion.

27. The embryo transfer device according to claim 25, characterized in that The acceleration is multiple accelerations.

28. The embryo transfer device according to claim 27, characterized in that The number of accelerations is 1-500 times.

29. The embryo transfer device according to claim 1, wherein: The invention also comprises a negative pressure device for sucking the liquid in the test tube into the sleeve.

30. The embryo transfer device according to claim 29, wherein: The negative pressure device is a manual negative pressure device or an automatic negative pressure device (50).

31. The embryo transfer device according to claim 30, wherein: The manual negative pressure device is one of a syringe, a rubber-tipped straw or an oral straw, and the automatic negative pressure device is one of a syringe pump, a peristaltic pump, a plunger pump, a diaphragm pump, a vacuum pump, a centrifugal pump, an impeller pump, and a piston pump.

32. The embryo transfer device according to claim 30, wherein: The negative pressure device absorbs the liquid in the test tube and injects it into the sleeve, so that at least one section of liquid is formed in the sleeve.

33. The embryo transfer device according to claim 32, wherein: The liquid is one of a culture fluid, a transplantation fluid or an operating fluid.

34. The embryo transfer device according to claim 31, wherein: The automatic negative pressure device (50) comprises a housing (51), a circuit board (52) and a motor (53), wherein the circuit board (52) and the motor (53) are installed in the housing (51), a control button (54) and a display screen (55) are provided on the surface of the housing (51), and the motor (53), the control button (54) and the display screen (55) are electrically connected to the circuit board (52) respectively.

35. The embryo transfer device according to claim 31, wherein: The automatic negative pressure device (50) has a liquid inlet and a liquid outlet. The liquid inlet is connected to an external test tube through a first connecting tube, and the liquid outlet is connected to the sleeve (30) through a second connecting tube (56). The automatic negative pressure device absorbs liquid in the test tube through negative pressure and injects the liquid into the sleeve.

36. A method for embryo transfer, characterized in that: The method comprises the following steps: a. Aspirate the liquid in the test tube to form at least one section of liquid in the cannula; b. Insert the front end sheet of the carrier rod into the sleeve, so that the carrier rod and the sleeve are respectively engaged with the clamp; c. Remove the sleeve from the fixture; d. Aspirate the liquid in the test tube again to form at least one section of liquid in the sleeve.

37. The embryo transfer method according to claim 36, wherein: Before step a, the method further comprises the following steps: inserting the cannula into the rubber stopper, and the test tube contains liquid.

38. The embryo transfer method according to claim 36, wherein: The method of aspirating the liquid in the test tube in step a can be manual or automatic.

39. The embryo transfer method according to claim 36, wherein: After step a, the method further comprises the following steps: placing the syringe and the cannula on a fixture and adjusting the relative position of the liquid in the cannula.

40. The embryo transfer method according to claim 36, wherein: In step b, the carrier rod and / or sleeve and the clamp generate acceleration through relative force, so that the embryo on the carrier rod falls into the sleeve.

41. The embryo transfer method according to claim 36, wherein: The following steps are also included after step d: taking the sleeve out of the rubber plug and putting it into the transplant gun for embryo transplantation.

42. The embryo transfer method according to claim 40, wherein: The relative acting force is friction force and / or elastic force generated by deformation.

43. The embryo transfer method according to claim 36, wherein: The liquid is one of a culture fluid, a transplantation fluid or an operating fluid.

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